Full-automatic blood sample pretreatment experimental device

Abstract

The invention relates to a full-automatic blood sample pretreatment experiment device, and relates to the technical field of pretreatment experiment devices, and the full-automatic blood sample pretreatment experiment device comprises a tube rack module which comprises a blood collection tube rack, a centrifugal tube rack, a suction head plate and a reagent bottle rack; the mechanical arm module comprises a carrying mechanical arm and a pipetting mechanical arm which can move in the X, Y and Z directions; the reverse cover pulling module comprises a reverse assembly and a cover pulling assembly; the reversing assembly is used for driving the blood collection tube to rotate in the vertical direction; the cover pulling assembly is used for clamping a blood collection tube; the cap screwing module comprises a cap screwing air bag and a driving assembly, the cap screwing air bag is used for clamping the centrifugal tube, and the driving assembly is used for driving the cap screwing air bag to rotate in the horizontal direction; the vortex module is used for carrying out vortex uniform mixing operation; the centrifugal module is used for conducting centrifugal operation. By arranging the experimental device capable of automatically pretreating the blood sample, the problem that errors are easy to introduce in the prior art is effectively solved, the treatment efficiency is improved, and the experimental requirements of a large number of samples are met.

Description

Technical Field

[0001] The present application relates to the field of pre-processing experimental devices, and in particular to a fully automatic blood sample pre-processing experimental device. Background Art

[0002] As the core carrier of medical testing, blood samples have become the most commonly used test sample in clinical diagnosis due to their easy accessibility and high efficiency in monitoring physiological status. With the development of immunoassay technology, fully automatic chemiluminescence immunoassay analyzers, with their advantages of high sensitivity and high specificity, are widely used in the detection of key items such as tumor markers, hormones, and infectious disease markers.

[0003] However, existing technologies still face significant bottlenecks: First, sample pretreatment relies on and has limitations. While current chemiluminescence immunoassay analyzers have automated testing, blood samples still require manual pretreatment steps such as centrifugation, aliquoting, and dilution before loading. Manual operations can easily introduce errors, directly affecting test accuracy, leading to false results or duplicate testing, and increasing medical costs. Second, there are gaps in automated processes. While modern fully automated analyzers (such as chemiluminescence analyzers and biochemical analyzers) are designed with blood testing in mind, pretreatment steps have not yet been integrated into automated processes. Manually transferring samples to micro-volume cuvettes is not only inefficient (especially in high-volume testing scenarios), but can also cause equipment failure due to clogging of the instrument's sampling needle by the separating gel in the sample tube. Finally, there are challenges with quality control. Existing technologies lack standardized control over pretreatment processes. For example, serum from patients receiving isomeric drugs may interfere with antigen-antibody reactions. Such issues require retrospective analysis of abnormal results and cannot be identified during the pretreatment phase. Furthermore, as clinical laboratories face the pressure of surging sample volumes, manual pretreatment becomes a bottleneck in testing efficiency. Summary of the Invention

[0004] In view of the above problems, the present application is proposed to provide a fully automatic blood sample pre-processing experimental device that overcomes the above problems or at least partially solves the above problems.

[0005] The fully automatic blood sample pretreatment experimental device provided in this application adopts the following technical solutions: A fully automatic blood sample pre-processing experimental device comprises a workbench and a tube rack module, a mechanical arm module, an inverted capping module, a capping module, a vortex module and a centrifugal module arranged on the workbench; The tube rack module includes at least one blood collection tube rack for storing blood collection tubes, at least one centrifuge tube rack for storing centrifuge tubes, at least one tip plate for storing tips, and at least one reagent bottle rack for placing reagent bottles; The robotic arm module includes a transport robotic arm and a pipetting robotic arm that can move in the X, Y, and Z directions; the transport robotic arm is used to transport the tube from one module to another; the pipetting robotic arm is used to transfer the sample liquid or reagent from one tube to another; The inversion and capping module includes an inversion component and a capping component; the inversion component is used to drive the blood collection tube to rotate in the vertical direction to perform a mixing operation; the capping component is used to clamp the blood collection tube and cooperate with the transport robot arm to complete the capping operation; The capping module includes an inflatable and deflable capping airbag and a driving assembly, wherein the capping airbag is used to clamp the centrifuge tube, and the driving assembly is used to drive the capping airbag to rotate horizontally; The vortex module is used to perform a vortex mixing operation, and includes a supporting plate for supporting the tube body and an eccentric component for driving the supporting plate to rotate eccentrically; The centrifugal module is used for performing centrifugal operation.

[0006] Optionally, the transport robot arm includes a carrier frame, a swing motor and a transport clamp; The swing motor is arranged on the carrier and is used to drive the transport clamp to deflect a certain angle in the vertical direction, and the transport clamp is equipped with a retractable clamp; The pipetting robot arm includes at least one pipetting needle and at least one pipetting pump, and the pipetting pump is correspondingly connected to the pipetting needle.

[0007] Optionally, the transport robot arm and the pipetting robot arm share an X-direction moving component.

[0008] Optionally, the inversion assembly includes a rotating platform that can move in the horizontal and vertical directions and a cap-pulling clamping claw arranged on the rotating platform, and the rotating platform is used to drive the cap-pulling clamping claw to rotate in the vertical direction; The capping assembly comprises an inflatable and deflable capping airbag, which is used for clamping the tube body.

[0009] Optionally, the workbench is provided with blood collection tube bases having a number corresponding to the blood collection tube racks, and the blood collection tube racks and the blood collection tube bases are movably connected.

[0010] Optionally, the blood collection tube rack is provided with a clamping position for use with the transport robot arm.

[0011] Optionally, a barcode scanner is further provided on the workbench, and the barcode scanner is arranged relative to the blood collection tube rack; The blood collection tube stand is provided with a scanning hole corresponding to and communicating with the blood collection tube hole position, and a spring piece for resisting the blood collection tube is also provided in the blood collection tube hole position.

[0012] Optionally, the centrifuge tube rack includes a centrifuge base, a first loading platform and a plurality of second loading platforms for placing centrifuge tubes, which are arranged sequentially from bottom to top; The first carrying platform is movably connected to the centrifugal base, and a plurality of the second carrying platforms are arranged side by side on the first carrying platform and are all movably connected to the first carrying platform.

[0013] Optionally, the fully automatic blood sample pre-processing experimental device further includes a tip recovery bin and at least one tip removal slot, and the tip removal slot is arranged above the tip recovery bin.

[0014] Optionally, it further comprises an auxiliary base provided on one side of the cap removal assembly, the auxiliary base being used for movably connecting the blood collection tube stand; Furthermore, the transporting mechanical arm (21) can transport the blood collection tube rack (11) between the blood collection tube base (7) and the auxiliary base (9).

[0015] In summary, the present application includes the following beneficial technical effects: By setting up an experimental device that can automatically perform pre-processing of blood samples, the present application effectively solves the problem of easy introduction of errors in the existing technology, improves processing efficiency, and adapts to the experimental needs of a large number of samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application.

[0017] Figure 2 Schematic diagram of the structure of the centrifuge tube rack in the embodiment of the present application.

[0018] Figure 3 It is a structural diagram of the upside-down cover removal module in an embodiment of the present application.

[0019] Explanation of the accompanying symbols: 1. Tube rack module; 11. Blood collection tube rack; 111. Clamping position; 112. Scanning hole; 113. Shrapnel; 12. Centrifuge tube rack; 121. Centrifuge base; 122. First carrying platform; 123. Second carrying platform; 13. Tip plate; 14. Reagent bottle rack; 2. Robotic arm module; 21. Transport robot arm; 211. Carrying frame; 212. Transport clamp; 213. Swing motor; 22. Pipetting robot arm; 3. Inversion and capping module; 31. Inversion assembly; 311. Rotating table; 312. Capping clamp; 32. Capping assembly; 321. Capping airbag; 4. Capping module; 41. Capping airbag; 42. Drive assembly; 5. Vortex module; 51. Carrying plate; 6. Centrifuge module; 7. Blood collection tube base; 8. Tip recovery bin; 81. Tip removal slot; 9. Auxiliary base; 10. Barcode scanner. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiment is only one embodiment of the present invention, not all embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, technical or scientific terms used in the present invention shall have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs.

[0022] This embodiment provides a fully automatic blood sample pretreatment experimental device.

[0023] Reference Figure 1 、 Figure 2 A fully automatic blood sample pretreatment experimental device includes a workbench and a tube rack module 1, a robotic arm module 2, an inverted capping module 3, a capping module 4, a vortex module 5 and a centrifugal module 6 arranged at corresponding workstations on the workbench.

[0024] The tube rack module 1 includes at least one blood collection tube rack 11 for storing blood collection tubes, at least one centrifuge tube rack 12 for storing centrifuge tubes, at least one tip plate 13 for storing tips, and at least one reagent bottle rack 14 for placing reagent bottles.

[0025] In order to facilitate the experimenters to take and put the blood sampling tube rack 11, the workbench is provided with the same number of blood sampling tube bases 7 as the blood sampling tube racks 11, and the blood sampling tube bases 7 are movably connected to the blood sampling tube racks 11. The blood sampling tube bases 7 are correspondingly provided with detection sensors for identifying whether there is a sampling tube rack at the workstation.

[0026] The centrifuge tube rack 12 comprises, arranged from bottom to top, a centrifuge base 121, a first loading platform 122, and multiple second loading platforms 123 for placing centrifuge tubes. The first loading platform 122 is movably connected to the centrifuge base 121. The multiple second loading platforms 123 are arranged side by side on the first loading platform 122 and are all movably connected to the first loading platform 122 to facilitate the partial or complete removal and placement of centrifuge tubes.

[0027] During the specific implementation process, the movable connection between the blood collection tube base 7 and the blood collection tube rack 11, the centrifugal base 121 and the first supporting platform 122, and the first supporting platform 122 and the second supporting platform 123 can all be achieved by means of card strip card slot connection, magnetic adsorption, etc.

[0028] The reagent bottle rack 14 is provided with an opening and closing cover for opening and closing the mouth of the reagent bottle. The opening and closing of the opening and closing cover can be achieved by a control mechanism such as a cylinder and a push rod.

[0029] Reference Figure 1 The robotic arm module 2 includes a transport robotic arm 21 and a pipetting robotic arm 22 .

[0030] The transport robot 21 is used to transport the corresponding tube body from one module to another module so that the corresponding module can perform corresponding processing operations. It includes a carrier 211 that can move along the X, Y, and Z directions and a transport clamp 212 arranged on the carrier 211. The transport clamp 212 is equipped with a retractable clamp for clamping the tube body (that is, the clamp can shrink or expand in the horizontal direction to realize the operation of clamping the tube body).

[0031] The pipetting robot 22 is used to transfer sample liquid or reagent from one tube body to another tube body, and includes at least one pipetting needle that can move along the X, Y, and Z directions and at least one pipetting pump; The pipetting needle can be detachably connected to the pipette tip stored on the pipette tip plate 13, and the pipetting needle is connected to the pipetting pump to achieve pipetting operation while preventing cross contamination.

[0032] In the specific implementation process, two pipetting needles are preferably provided, one pipetting needle is used to transfer reagents, and the other pipetting needle is used to transfer blood samples, so as to further avoid the occurrence of cross contamination.

[0033] In order to facilitate the disassembly and collection of used tips, the workbench is also provided with a tip recovery bin 8 and at least one tip disassembly groove 81. The tip disassembly groove 81 is arranged above the tip recovery bin 8 and is tapered. It is used to clamp the top edge position of the tip to cooperate with the Z-axis motion component to realize the disassembly of the tip.

[0034] In a specific implementation, in order to save space and cost, the transport robot arm 21 and the pipetting robot arm 22 may share an X-direction moving component.

[0035] Reference Figure 1 、 Figure 3 The inverting and uncapping module 3 includes an inverting component 31 and a uncapping component 32 .

[0036] The inversion assembly 31 is used to drive the blood collection tube to rotate in the vertical direction to achieve the inversion and mixing operation of the sampled blood; the inversion assembly 31 includes a rotating table 311 and a capping clamp 312. The rotating table 311 can move in the horizontal and vertical directions, and the rotating table 311 itself can rotate in the vertical direction. The capping clamp 312 is set on the rotating table 311 and rotates with the rotating table 311 to clamp the blood collection tube and rotate it in the vertical direction to achieve the inversion and mixing operation.

[0037] The capping assembly 32 is located at the next station of the inverting assembly 31 and is used to cooperate with the transport robot 21 to perform the capping or closing operation of the blood collection tube, so as to realize the opening and closing control of the blood collection tube.

[0038] The capping assembly 32 includes an inflatable and deflable capping airbag 321 for clamping the tube body. After the blood collection tube is inverted and mixed, it is placed in the capping airbag 321 and the capping clamping claw 312 is used to open the tube.

[0039] In order to improve the efficiency of inversion and cap removal, the workbench is also provided with an auxiliary base 9 located on one side of the cap removal assembly 32. The auxiliary base 9 is used to place the blood collection tube rack 11 and is movably connected to the blood collection tube rack 11; at the same time, the blood collection tube rack 11 is also provided with a clamping position 111 adapted to the clamping claws of the transport robot arm 21, so that the transport robot arm 21 can transport the blood collection tube rack 11 between the blood collection tube base 7 and the auxiliary base 9.

[0040] 1, the capping module 4 is used to perform capping operations on the centrifuge tubes transported by the transport robot arm 21, thereby realizing the opening and closing control of the centrifuge tube caps.

[0041] The capping module 4 includes a capping airbag 41 and a driving assembly 42. The capping airbag 41 can be inflated and deflated and is used to clamp the centrifuge tube transported by the transport robot 21; the driving assembly 42 is used to drive the capping airbag 41 to rotate horizontally.

[0042] When opening or closing the centrifuge tube, the transport robot arm 21 inserts the centrifuge tube into the capping airbag 41, the capping airbag 41 is inflated and clamps the centrifuge tube, and then the driving component 42 controls the capping airbag 41 to rotate as a whole in the corresponding direction. At this time, the transport robot arm 21 is in a clamping state on the centrifuge tube cap, so the centrifuge tube can be opened and closed during the rotation of the capping airbag 41.

[0043] Reference Figure 1 The vortex module is located at the next station of the capping module 4 and is used for performing vortex mixing operations. It includes a carrier plate 51 for carrying the centrifuge tube and an eccentric component for driving the carrier plate 51 to rotate (the eccentric component includes a motor and an eccentric rotor arranged on the motor, which is an existing mature technical structure and will not be described in detail in this application).

[0044] When performing a vortex mixing operation, the transport robot arm 21 transports the centrifuge tube to the carrying tray and makes the bottom of the centrifuge tube contact the carrying plate 51. Then the eccentric component is started, and at the same time, the clamping claws of the transport robot arm 21 extend outward a certain distance, leaving a certain movement gap at the top of the centrifuge tube, and the vortex mixing operation can be performed.

[0045] Reference Figure 1 The centrifugal module 6 is located at the next station of the vortex module 5 and is used for performing centrifugal operation.

[0046] The centrifugal module 6 includes a centrifuge.

[0047] Since the hole of the centrifugal module 6 for placing the centrifuge tube is set at an angle, in order to facilitate the insertion of the centrifuge tube into the hole, the transport robot 21 also includes a swing motor 213 arranged between the carrier 211 and the transport clamp 212, and the swing motor 213 is used to drive the transport clamp 212 to deflect a certain angle in the vertical direction.

[0048] In the specific implementation process, the centrifuge is also hinged with a protective cover, and the opening and closing of the protective cover is achieved through control mechanisms such as a cylinder and a push rod.

[0049] Reference Figure 1 、 Figure 3 A code scanner 10 is also provided on the workbench. The code scanner 10 is arranged opposite to the blood collection tube rack 11 and is used to scan the code information on the blood collection tube.

[0050] In order to facilitate the code scanner 10 to accurately scan the sampling blood vessel code, a scanning hole 112 is provided on the blood sampling blood vessel stand 11 and is connected to the corresponding blood sampling blood vessel hole position; at the same time, a spring piece 113 for resisting the blood sampling blood vessel is also provided in the blood sampling blood vessel hole position to prevent the blood sampling blood vessel placed in the hole position from rotating, affecting the alignment of the code area and the code scanner 10, so as to further ensure the accuracy of the code scanner 10 in scanning the sampling blood vessel code information.

[0051] The workflow of a fully automatic blood sample pre-processing experimental device in this embodiment of the application is as follows: Step 1: Install the blood collection tube rack 11 on the blood collection tube base 7 in sequence, and install them in sequence from far away from the code scanner 10 to close to the code scanner 10 to ensure that the code scanner 10 accurately scans the code information on the blood collection tube; Step 2: The transporting robot arm 21 transports the blood collection tube rack 11 and installs it on the auxiliary base 9; Step 3: The uncapping clamp 312 grabs the blood collection tube and removes it from the blood collection tube. Then, the rotating platform 311 drives the blood collection tube to rotate in the vertical direction to complete the inversion and mixing operation. Step 4: The capping clamp 312 places the inverted and mixed blood collection tube into the capping airbag 321. The capping airbag 321 is inflated to clamp the blood collection tube, and the capping clamp 312 moves upward to remove the tube cap. Step 5: Take the tips of two pipette needles, then use one pipette needle to draw a certain amount of reagent into the reagent bottle, and use another pipette needle to draw a certain amount of sample into the blood collection tube; Step 6: The transport robot 21 transports the centrifuge tube to the capping module 4 to open the cap, and the pipette needle adds reagents and samples into the centrifuge tube; Step 7: Close the cap of the centrifuge tube and transport the centrifuge tube to the vortex module 5 for vortex mixing; Step 8: The transport robot 21 transports the centrifuge tube after vortex mixing to the centrifuge, and the centrifuge closes the protective cover to complete the centrifugation operation; Step 9: The transport robot 21 transports the centrifuge tube back to the centrifuge tube rack 12, completing the pre-processing process.

[0052] The movement of the relevant structures or components in this application along the horizontal and vertical (or X, Y, Z) directions can be achieved through common structures such as ball screws / trapezoidal screws, synchronous belts / toothed belts, gear racks, hydraulic cylinders, and pneumatic cylinders; rotational movement can be achieved through common structures such as gears, pulleys, and motors. You can choose the appropriate structure according to the specific scenario.

[0053] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A fully automatic blood sample pre-treatment experimental device, characterized by: It comprises a workbench and a tube rack module (1) arranged on the workbench, a robotic arm module (2), an inverted capping module (3), a capping module (4), a vortex module (5) and a centrifugal module (6); The tube rack module (1) comprises at least one blood collection tube rack (11) for storing blood collection tubes, at least one centrifuge tube rack (12) for storing centrifuge tubes, at least one pipette tip plate (13) for storing pipette tips, and at least one reagent bottle rack (14) for placing reagent bottles. The robotic arm module (2) comprises a transport robotic arm (21) and a liquid transfer robotic arm (22) that can move in the X, Y, and Z directions; the transport robotic arm (21) is used to transport a tube from one module to another; the liquid transfer robotic arm (22) is used to transfer a sample liquid or a reagent from one tube to another; The inversion and capping module (3) comprises an inversion assembly (31) and a capping assembly (32); the inversion assembly (31) is used to drive the blood collection tube to rotate in a vertical direction to perform a mixing operation; the capping assembly (32) is used to clamp the blood collection tube and cooperate with the transport robot arm (21) to complete the capping operation; The capping module (4) comprises an inflatable and deflable capping airbag (41) and a driving assembly (42), wherein the capping airbag (41) is used to clamp the centrifuge tube, and the driving assembly (42) is used to drive the capping airbag (41) to rotate in a horizontal direction; The vortex module (5) is used to perform a vortex mixing operation, and comprises a supporting plate (51) for supporting the tube body and an eccentric component for driving the supporting plate (51) to rotate eccentrically; The centrifugal module (6) is used for performing centrifugal operation.

2. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The transporting mechanical arm (21) comprises a carrier (211), a swing motor (213) and a transporting clamp (212); The swing motor (213) is arranged on the carrier (211) and is used to drive the transport clamp (212) to deflect a certain angle in the vertical direction, and the transport clamp (212) is equipped with a retractable clamp; The pipetting mechanical arm (22) comprises at least one pipetting needle and at least one pipetting pump, and the pipetting pump is correspondingly connected to the pipetting needle.

3. The fully automatic blood sample pre-treatment experimental device according to claim 1 or 2, characterized in that: The transporting robot arm (21) and the liquid transfer robot arm (22) share an X-direction moving component.

4. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The inversion assembly (31) comprises a rotating platform (311) movable in horizontal and vertical directions and a cap-pulling clamp (312) disposed on the rotating platform (311), and the rotating platform (311) is used to drive the cap-pulling clamp (312) to rotate in the vertical direction; The cap-pulling assembly (32) comprises an inflatable and deflable cap-pulling airbag (321), and the cap-pulling airbag (321) is used for clamping the tube body.

5. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The workbench is provided with a number of blood collection tube bases (7) corresponding to the number of the blood collection tube racks (11), and the blood collection tube racks (11) and the blood collection tube bases (7) are movably connected.

6. The fully automatic blood sample pre-treatment experimental device according to claim 5, characterized in that: The blood collection tube rack (11) is provided with a clamping position (111) for use in conjunction with the transporting mechanical arm (21).

7. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The workbench is also provided with a code scanner (10), and the code scanner (10) is arranged relative to the blood collection tube stand (11); The blood collection tube stand (11) is provided with a scanning hole (112) corresponding to and communicating with the blood collection tube hole position, and a spring piece (113) for resisting the blood collection tube is also provided in the blood collection tube hole position.

8. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The centrifuge tube rack (12) comprises a centrifuge base (121), a first carrying platform (122) and a plurality of second carrying platforms (123) for placing centrifuge tubes, which are arranged in sequence from bottom to top; The first carrying platform (122) and the centrifugal base (121) are movably connected, and a plurality of the second carrying platforms (123) are arranged side by side on the first carrying platform (122) and are all movably connected to the first carrying platform (122).

9. The fully automatic blood sample pre-treatment experimental device according to claim 1, characterized in that: The fully automatic blood sample pre-processing experimental device further comprises a tip recovery bin (8) and at least one tip removal slot (81), wherein the tip removal slot (81) is arranged above the tip recovery bin (8).

10. The fully automatic blood sample pre-treatment experimental device according to claim 5, characterized in that: It also includes an auxiliary base (9) arranged on one side of the cap removal assembly (32), and the auxiliary base (9) is used for movably connecting the blood collection tube stand (11); Furthermore, the transporting mechanical arm (21) can transport the blood collection tube rack (11) between the blood collection tube base (7) and the auxiliary base (9).

Images