Bionic gastrointestinal digestive system in lunar environment

Abstract

The invention relates to a bionic gastrointestinal digestive system in a lunar environment, and belongs to the technical field of bionic digestive systems. The bionic stomach digestion system, the bionic small intestine digestion system and the bionic colon fermentation system which are suspended in the water tank are arranged and are circularly connected to form the bionic gastrointestinal digestion system in the simulated moon environment; according to the astronaut gastrointestinal tract digestion simulation device, the stomach metabolism condition, the small intestine metabolism condition and the intestinal flora distribution and metabolism condition in the colon of an astronaut in a weightless environment can be simulated, the gastrointestinal tract digestion problem of the astronaut can be deeply studied, spaceflight food is developed, automation is achieved, and according to a preset program, the astronaut gastrointestinal tract digestion problem can be effectively solved. The PLC controls the addition of a sample, digestive juice and a buffer solution, and simulates the wriggling and emptying of a gastric digestive system, so that the operation is more convenient.

Description

Technical Field

[0001] The present invention relates to a bionic gastrointestinal digestive system in a lunar environment, belonging to the technical field of bionic digestive systems. Background Art

[0002] The moon is the first island in the cosmic ocean after Earth, the continental landmass. The distance between Earth and the moon is only 380,000 kilometers, one-hundredth to one-thousandth of the distance between Earth and Mars. With current human science and technology, utilizing Mars remains a distant prospect. The moon, however, is a present and tangible reality. It is not only an ideal base for scientific research, but also a treasure trove of resource development and utilization, and a springboard and outpost for reaching deeper space.

[0003] With the announcement of my country's lunar landing plan, public attention has begun to focus on manned lunar landing technology and the challenges that need to be overcome in planning and constructing a lunar research station or base. To ensure that astronauts can live normally and maintain healthy vital signs in a lunar research station, it is necessary to study the in vitro digestion process in the lunar environment.

[0004] Currently, existing in vitro digestion technologies typically use static digestion or dynamic simulated digestion devices. Static model reactors have the advantages of simple structure, easy operation, and low cost, and are currently widely used. A common static model reactor is to place a beaker on a constant temperature magnetic stirrer; or place a conical flask in a constant temperature shaking water bath, simulating gastrointestinal motility through stirring or shaking. However, static model reactors cannot simulate the dynamic digestion process in the human gastrointestinal tract and cannot control a strict anaerobic environment.

[0005] Dynamic model reactors can simulate physical processes such as shear and mixing in the gastrointestinal tract. They also have functions such as pH control, material addition, digestive fluid secretion, and metabolite absorption. Currently, there are three main types of dynamic model reactors. The TIM is the in vitro dynamic model reactor that most closely resembles the human gastrointestinal digestive system. It consists of the TNO gastro-intestinal model (TIM-1) and the TNO in vitro model of the colon (TIM-2). The TIM-2 consists of four interconnected glass containers forming a circulation loop. The interior of the TIM-2 is an elastic wall, with circulating water (at a temperature of 37°C when simulating the human body) flowing between the glass and elastic walls. Periodic pressure is applied to the circulating water, causing the elastic walls to contract and generate peristaltic waves, which mix the contents and allow them to flow within the reactor.

[0006] The domestic biomimetic gastric reactor (BGR) consists of three consecutive glass reaction vessels arranged according to the gastric fundus, gastric body, and gastric antrum. The compartment reactor is equipped with a silicone stomach. The intermediate components include an alkali addition port, a feed port, a vacuum port, a sample addition port, a water suction device, a dialysis reflux port, and a pH electrode port. The space between the silicone stomach and the compartment wall is filled with 37°C constant-temperature ultrapure water to simulate the actual human body temperature. In addition, a circulating water tank and a peristaltic pump periodically squeeze the water to simulate gastric peristalsis.

[0007] The domestic bionic small intestinal reactor (BSIR) consists of three consecutive glass reaction vessels arranged according to the duodenum, jejunum, and ileum, with a silicone small intestine installed in the compartment reactor. The intermediate components include an alkali addition port, a feed port, a vacuum port, a sample addition port, a water suction device, a dialysis reflux port, and a pH electrode port. The space between the silicone small intestine and the compartment wall is filled with 37°C constant-temperature ultrapure water to simulate the actual human body temperature. Furthermore, a circulating water tank and a peristaltic pump periodically squeeze the water to simulate small intestinal peristalsis.

[0008] The domestic bionic large intestinal reactor (BLIR) consists of three continuous glass reaction vessels arranged according to the ascending colon, transverse colon, and descending colon, with a compartment reactor equipped with a silicone colon. The intermediate components include an alkali addition port, a feed port, a vacuum port, a nitrogen filling port, a sample addition port, a water suction device, a dialysis reflux port, and a pH electrode port. The space between the silicone colon and the compartment wall is filled with 37°C constant-temperature ultrapure water to simulate the actual temperature of the human body. In addition, a circulating water tank and a peristaltic pump periodically squeeze the water to simulate colonic peristalsis.

[0009] However, the above-mentioned in vitro stomach simulation digestion devices, small intestine simulation digestion devices, and colon simulation digestion devices are not only unable to accurately simulate the peristalsis of the stomach, small intestine, and colon and the internal digestion process in a weightless environment, but also cannot simulate the peristalsis of the stomach, small intestine, and colon and the internal digestion process in a lunar environment. They cannot provide useful and reliable data for studying the gastrointestinal health of astronauts who will land on the moon in the future. Moreover, the liquid extrusion peristaltic digestion and the movement in the human stomach are quite different from the actual situation, and cannot simulate the real stomach, small intestine, and colon well.

[0010] At the same time, the existing technology models have the technical defect of being unable to simulate the real irregular food flow in the stomach and intestines during the peristaltic simulation process. For example, the published patent CN108318625A has only one water outlet and one water inlet. When simulating peristalsis, the bionic silicone membrane is only subjected to vertical force, resulting in poor simulation of the irregular food flow process, which limits the future research on in vitro digestion in the lunar environment. Summary of the Invention

[0011] In order to solve the above problems, the present invention provides a bionic gastrointestinal digestive system for lunar environment, comprising interconnected:

[0012] A bionic gastric digestion system, comprising a bionic gastric digestion model suspended in a first water tank and a first sampling system connected to the bionic gastric digestion model, wherein the bionic gastric digestion model comprises a spherical reactor, wherein the spherical reactor is connected to a symmetrically arranged first cylindrical reactor and a second cylindrical reactor via a flange and an intermediate body, wherein the spherical reactor, the first cylindrical reactor, and the second cylindrical reactor all have an air inlet and an air outlet, and the first cylindrical reactor and the second cylindrical reactor each have at least three air inlets and at least three air outlets; and

[0013] A bionic small intestinal digestion system, comprising a bionic small intestinal digestion model suspended in a second water tank and a second sampling system connected to the bionic small intestinal digestion model, wherein the bionic small intestinal digestion model comprises a duodenum module, a jejunum module, and an ileum module having identical structures and connected in parallel, wherein the duodenum module comprises a straight reactor, and a third and fourth tubular reactors respectively connected to both ends of the straight reactor via flanges, wherein the third and fourth tubular reactors each have at least three air inlets and at least three air outlets, and the air outlets and air inlets can be arranged upward and downward, respectively; and

[0014] A bionic colon fermentation system, comprising a bionic colon fermentation model suspended in a third water tank and a third sampling system connected to the bionic colon fermentation model. The bionic colon fermentation model comprises an ascending colon module, a transverse colon module, and a descending colon module having identical structures and connected in parallel. The ascending colon module comprises an arc reactor, and a fifth and sixth cylindrical reactors respectively connected to both ends of the arc reactor via flanges. The fifth and sixth cylindrical reactors each have at least three air inlets and at least three air outlets.

[0015] Among them, the first sampling system, the second sampling system, and the third sampling system are all connected to the circulating gas box to form a circulation; the first water tank, the second water tank, and the third water tank all have buoyant transparent or translucent liquid, and the density of the liquid is 2-3 mol / L; the bionic small intestine digestion system and the bionic colon fermentation system are both provided with a liquid level sensor for measuring the liquid level in the reaction liquid. The purpose is: first, the reaction gas will compress the liquid surface, and the gas production can be monitored in real time by the amount of liquid level drop; second, the drop in liquid level will cause the electrode to be exposed outside the liquid surface and cannot receive the signal well. The liquid level sensor can monitor the drop in liquid level in real time to prevent the electrode from losing the signal.

[0016] The bionic gastrointestinal digestive system is controlled by a PLC; the bionic stomach digestive system is connected to the second sampling system by a first inter-system connecting pump, and the bionic small intestine digestive system is connected to the third sampling system by a second inter-system connecting pump, and the third sampling system is also connected to the bionic stomach digestive system.

[0017] Furthermore, the first sampling system includes a first peristaltic pump, a first sample bottle connected to the first peristaltic pump, and a first acid and base bottle, wherein the first peristaltic pump is connected to two intermediates;

[0018] The second sampling system includes a second peristaltic pump, a second sample bottle connected to the second peristaltic pump, and a second acid and base bottle, and the second peristaltic pump is connected to the straight reactor;

[0019] The third sampling system includes a third peristaltic pump, a third sample bottle and a third acid and alkali bottle, and a gas bottle connected to the third peristaltic pump, and the third peristaltic pump is connected to the arc reactor;

[0020] The first peristaltic pump, the second peristaltic pump, and the third peristaltic pump are connected to each other and are all connected to a circulating air box.

[0021] Furthermore, the intermediate has a first injection port and a first electrode port, the first injection port is connected to the first peristaltic pump, and the first electrode port is inserted with a pH electrode;

[0022] The straight reactor has a second injection port, a second electrode port, a second microscope port, and a second tail gas analysis port, the second injection port and the second acid and alkali addition port are connected to the second peristaltic pump, the second electrode port is inserted with a pH electrode, and the second microscope port is inserted with a microscope probe;

[0023] The arc-shaped reactor has a third sampling port, a third electrode port, a third microscope port, and a third exhaust gas analysis port. The third sampling port is connected to the third peristaltic pump, the third electrode port is inserted with a pH electrode, the third microscope port is inserted with a microscope probe, and the exhaust gas analysis port is inserted with an exhaust gas analysis probe. The exhaust gas analysis probe can detect the component concentration of the mixed gas generated by the reaction in the arc-shaped reactor.

[0024] It should be understood that the pH electrode is used to measure the pH conditions in each reactor or intermediate body to coordinate with the feeding system for adjustment; the microscope probe is used to detect the degree of digestion of the digestate at a certain time to better study the digestion conditions and observe the growth of bacteria in the colon reactor; the exhaust gas analysis probe can detect the component concentrations of the mixed gas produced by the reaction in the arc reactor, including but not limited to H2 concentration, CO2 concentration, NO concentration, CH4 concentration, H2S concentration, VOC concentration, NH3 concentration and other gases.

[0025] In one embodiment of the present invention, the gastric digestion model is provided with a bionic silicone gastric tract that branches from the inner cavity of the spherical reactor and extends to the inner cavity of the first cylindrical reactor and the inner cavity of the second cylindrical reactor. The bionic silicone gastric tract extends from the inner cavity of the first cylindrical reactor and the inner cavity of the second cylindrical reactor and is fixed to the outlet of the first cylindrical reactor and the outlet of the second cylindrical reactor. The outlet of the first cylindrical reactor and the outlet of the second cylindrical reactor are located at the end of the first cylindrical reactor and the second cylindrical reactor away from the intermediate body; the outlet of the first cylindrical reactor and the outlet of the second cylindrical reactor are both connected to an adapter and an output tube, and the output tube is connected to a Luer connector; the third cylindrical reactor, the fourth cylindrical reactor, the fifth cylindrical reactor, and the sixth cylindrical reactor are all provided with a bionic silicone intestine.

[0026] It should be understood that the bionic silicone gastric tract and bionic silicone intestine are elastic bag structures made of silicone material, which can be turned outward and fixed on the outside of the flange; the purpose of providing the Luer connector is to facilitate sampling.

[0027] In one embodiment of the present invention, the first cylindrical reactor and the second cylindrical reactor each have four gas outlets and four gas inlets and at least one gas outlet and at least one gas inlet are connected to a ball valve, and each of the ball valves is connected to a first temperature control system and a PLC control system; the spherical reactor has at least three gas inlets and is connected to a ball valve; the third cylindrical reactor and the fourth cylindrical reactor each have four gas outlets and four gas inlets and are connected to a ball valve, and each of the ball valves is connected to a second temperature control system and a PLC control system; the fifth cylindrical reactor and the sixth cylindrical reactor each have three gas outlets and three gas inlets and are connected to a ball valve, and each of the ball valves is connected to a third temperature control system and a PLC control system.

[0028] It should be understood that the purpose of setting ball valves on the air inlet and outlet is to facilitate the control of peristalsis; the temperature control system is set to control the temperature of the reactor to maintain it in the range of 37.5-38, in order to simulate the real human gastrointestinal temperature.

[0029] Furthermore, the first water tank, the second water tank and the third water tank are respectively floated with a first buoy, a second buoy and a third buoy, and the first buoy, the second buoy and the third buoy are respectively suspended with a bionic stomach digestive system, a bionic small intestine digestive system and a bionic colon fermentation system.

[0030] In one embodiment of the present invention, before feeding, the bionic stomach digestion system regulates the pH of the intermediate between the first cylindrical reactor and the spherical reactor to be within the range of 1.0-1.5, and the pH of the intermediate between the second cylindrical reactor and the spherical reactor to be within the range of 1.5-2.0 through the first feeding system; after feeding, the bionic stomach digestion system regulates the pH of the intermediate between the first cylindrical reactor and the spherical reactor to be within the range of 2.0-3.0, and the pH of the intermediate between the second cylindrical reactor and the spherical reactor to be within the range of 3.0-4.0 through the first feeding system;

[0031] After feeding, the bionic small intestine digestion system regulates the pH of the duodenum module, the jejunum module, and the ileum module to be within the range of 5.2-5.8, 5.8-6.5, and 6.5-7.8 respectively through the second feeding system;

[0032] After feeding, the bionic colon fermentation system regulates the pH of the transverse colon module, the descending colon module, and the ascending colon module to be within the range of 6.0-6.5, 6.5-7.0, and 7.0-7.5 respectively through the second feeding system.

[0033] Furthermore, the peristaltic compression frequency of the first and second cylindrical reactors is 3-5 times / minute, the air filling volume is 350-750 mL, the flow rate is 2-4 L / min, and the compression amplitude is 1.5-3.5 cm; the peristaltic compression frequency of the spherical reactor is 3-4 times / minute, the flow rate is 2-4 L / min, the air filling volume is 500 mL-1 L, and the compression amplitude is 2.5-4.5 cm;

[0034] The flow rate between the duodenum module and the jejunum module is 15-25 mL / min, the flow rate between the jejunum module and the ileum module is 35-65 mL / min, the peristaltic compression frequency of the third cylindrical reactor and the fourth cylindrical reactor is 3-5 times / minute, the air inflation volume is 350-750 L, the flow rate is 2-4 L / min, and the compression amplitude is 1.5-3.5 cm;

[0035] The flow rate between the ascending colon module and the transverse colon module is 8-18 mL / min, the flow rate between the transverse colon module and the descending colon module is 35-65 mL / min, the peristaltic compression frequency of the fifth cylindrical reactor and the sixth cylindrical reactor is 3-5 times / minute, the air inflation volume is 350-750 L, the flow rate is 2-4 L / min, and the compression amplitude is 1.5-3.5 cm;

[0036] The flow rate between the bionic stomach digestion system and the bionic small intestine digestion system is 15-25 mL / min; the flow rate between the bionic small intestine digestion system and the bionic colon fermentation system is 15-25 mL / min.

[0037] It should be understood that the present invention uses the buoyancy of the entire model through the liquid in the water tank to simulate the gravity exerted on the entire model in a weightless environment, and the liquid density parameters provided by the present invention are only for simulating the weightless environment of the lunar surface in a vacuum (approximately 1 / 6 of the Earth's gravity), and do not simulate the gravity of non-celestial areas, space stations, space capsules, etc. in a vacuum.

[0038] Due to the influence of the moon's gravity, gastrointestinal peristalsis needs to overcome more force than in non-celestial areas, space stations, space capsules and other environments in a vacuum. Therefore, in order to more realistically simulate actual peristalsis, the amplitude of gastric and intestinal peristalsis should not be too large; at the same time, for peristaltic structures such as the stomach and small intestine that flow downward, the flow rate should not be too small; but there is an ascending colon between the small intestine and the colon, and its flow rate will be slightly lower than that of the stomach and small intestine.

[0039] Beneficial effects of the present invention:

[0040] The present invention sets up a bionic stomach digestive system, a bionic small intestine digestive system, and a bionic colon fermentation system suspended in a water tank and establishes a loop connection to form a bionic gastrointestinal digestive system that simulates the lunar weightlessness environment. It can not only simulate the astronauts' metabolic conditions in the stomach, small intestine, and intestinal flora distribution and metabolism in the colon in the lunar weightlessness environment, and can conduct in-depth research on the astronauts' gastrointestinal digestion problems and develop space food, but also realizes automation. According to a pre-set program, the PLC can control the addition of samples, digestive fluid and buffer, and the peristalsis and emptying of the bionic stomach digestive system, making the operation more convenient. Compared with traditional simulated digestion devices, the present invention adopts a glass reactor with a flexible and elastic silicone bionic stomach inside, which can keep the sample warm. At the same time, the present invention sets multiple air inlets and outlets to form a circulating gas pressure between the glass bottle and the silicone bionic gastric tract and silicone bionic intestine, which squeezes the silicone bionic gastric tract and silicone bionic intestine to achieve a better reciprocating motion, thereby more realistically simulating the digestion of food under the peristalsis of the real intestine. In addition, the intermediate, straight reactor and arc reactor of the present invention are provided with multiple sockets, which realize real-time observation of the sample change process in the model and can directly obtain process data. The collection of the process data can provide a reference for optimizing the operating parameters of the model. Moreover, each part is relatively independent and convenient for disassembly and assembly. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic structural diagram of a bionic gastrointestinal digestive system in one embodiment of the present invention.

[0042] Figure 2 This is a schematic structural diagram of a bionic stomach digestive system in one embodiment of the present invention.

[0043] Figure 3 This is a top view of the structure of a bionic stomach digestive system in one embodiment of the present invention.

[0044] Figure 4 This is a schematic structural diagram of a bionic small intestine digestive system in one embodiment of the present invention.

[0045] Figure 5 This is a schematic diagram of the parallel connection of the duodenum module, the jejunum module, and the ileum module in one embodiment of the present invention.

[0046] Figure 6 This is a schematic structural diagram of a bionic colon fermentation system in one embodiment of the present invention.

[0047] Figure 7 This is a schematic diagram of the parallel connection of the ascending colon module, the transverse colon module, and the descending colon module in one embodiment of the present invention.

[0048] Figure 8 This is a graph showing the digestibility of reducing sugars in a bionic stomach digestive system according to one embodiment of the present invention.

[0049] Figure 9 This is a protein digestibility diagram of the bionic stomach digestive system in one embodiment of the present invention.

[0050] Figure 10 This is a diagram of fat digestibility in a bionic stomach digestive system according to one embodiment of the present invention.

[0051] Figure 11 Graph showing the crushing capacity of the bionic stomach digestive system in one embodiment of the present invention.

[0052] Figure 12 This is a graph showing protein absorption rate of a bionic small intestinal digestive system in one embodiment of the present invention.

[0053] Figure 13 This is a graph showing the reducing sugar absorption rate of a bionic small intestinal digestive system in one embodiment of the present invention.

[0054] Figure 14 This is a diagram of the fat absorption rate of the bionic small intestine digestive system in one embodiment of the present invention.

[0055] Figure 15 This is a graph showing the digestibility of reducing sugars in a bionic colonic fermentation system according to one embodiment of the present invention.

[0056] Figure 16 、 17 This is a gas production diagram of a bionic colon fermentation model in one embodiment of the present invention.

[0057] Figure 18 This is a diagram showing the proportion of beneficial bacteria in a bionic colon fermentation system in one embodiment of the present invention.

[0058] Figure 19 This is a diagram showing the metabolic yield of short-chain fatty acids in a bionic colon fermentation system in one embodiment of the present invention.

[0059] Figure 20 This is a physical model diagram of a bionic gastrointestinal digestive system separated from a water tank in one embodiment of the present invention.

[0060] In the figure, 1: spherical reactor, 2: first cylindrical reactor, 3: second cylindrical reactor, 4: intermediate, 5: intermediate, 6: biomimetic silicone gastric tract, 7: biomimetic silicone gastric tract, 8: adapter, 9: ball valve, 10: first temperature control system peristaltic pump, 11: first injection port, 12: first electrode port, 13: first injection port, 14: first electrode port, 15: first peristaltic pump, 16: air inlet, 17: air outlet, 18: first sample bottle, 19: first acid Alkali bottle, 20: First float, 21: First temperature control system, 22: Second sample inlet, 23: Straight reactor, 24: Third cylindrical reactor, 25: Fourth cylindrical reactor, 26: Second microscope port, 27: Second exhaust gas analysis port, 28: Second electrode port, 29: Bionic silicone intestine, 30: Second liquid level sensor, 31: Air inlet, 32: Air outlet, 33: Adapter, 34: Ball valve, 35: Second sample bottle, 36: Second acid and alkali bottle, 37: Second temperature control system peristaltic pump, 38: Second peristaltic pump, 39: Second buoy, 40: PLC control system, 41: Second temperature control system, 42: Arc reactor, 43: Fifth cylindrical reactor, 44: Sixth cylindrical reactor, 45: Third exhaust gas analysis port, 46: Third microscope port, 47: Third injection port, 48: Third electrode port, 49: Air inlet, 50: Air outlet, 51: Ball valve, 52: Adapter, 53: Third peristaltic pump, 54: Third Sample bottle, 55: Gas bottle, 56: Peristaltic pump of the third temperature control system, 57: Bionic silicone intestine, 58: PLC control system, 59: Third buoy, 60: Third acid and alkali bottle, 61: Solenoid valve, 63: Third liquid level sensor, 64: Third temperature control system, 65: Second intestinal connecting pump, 66: First intestinal connecting pump, 67: First inter-system connecting pump, 68: Second inter-system connecting pump, 71: First water sink, 72: Second water sink, 73: Third water sink. DETAILED DESCRIPTION

[0061] Example

[0062] The present invention provides a bionic gastrointestinal digestive system for a lunar environment, comprising interconnected:

[0063] like Figure 2 、 Figure 3 As shown, the bionic gastric digestion system includes a bionic gastric digestion model suspended in a first water tank 71, and a first sampling system connected to the bionic gastric digestion model, the bionic gastric digestion model includes a spherical reactor 1, the spherical reactor 1 is connected to a symmetrically arranged first cylindrical reactor 2 and a second cylindrical reactor 3 through a flange and an intermediate body 4, and a flange and an intermediate body 5, respectively, the spherical reactor 1, the first cylindrical reactor 2, and the second cylindrical reactor 3 all have an air inlet 16 and an air outlet 17, the first cylindrical reactor 2 and the second cylindrical reactor 3 all have four air outlets 17 and four air inlets 16, and at least one air outlet 17 and at least one air inlet 16 are connected to a ball valve 9, each of the ball valves 9 is connected to a first temperature control system 21 and a PLC control system, and the spherical reactor has three air inlets 16 and one air outlet 17, and all are connected to the ball valve 9;

[0064] like Figure 4 、 Figure 5 As shown, the bionic small intestine digestion system includes a bionic small intestine digestion model suspended in a second water tank 72, and a second sampling system connected to the bionic small intestine digestion model. The bionic small intestine digestion model includes a duodenum module, a jejunum module, and an ileum module with the same structure and connected in parallel. The duodenum module includes a straight reactor 23a, a third cylindrical reactor 24a and a fourth cylindrical reactor 25a respectively connected to the two ends of the straight reactor 23a through flanges, and the jejunum module includes a straight reactor 23b, a third cylindrical reactor 24a and a fourth cylindrical reactor 25a respectively connected to the two ends of the straight reactor 23b through flanges. The ileum module includes a straight reactor 23c, a third cylindrical reactor 24c and a fourth cylindrical reactor 25c respectively connected to both ends of the straight reactor 23c by flanges, the third cylindrical reactor 23 and the fourth cylindrical reactor 24 each have four gas outlets 32 and four gas inlets 31 and are connected to a ball valve, each of the ball valves 34 is connected to the second temperature control system 41 and the PLC control system 40, and the gas outlet 32 ​​and the gas inlet 31 are respectively arranged upward and downward; and

[0065] like Figure 6 、 Figure 7As shown, the bionic colon fermentation system includes a bionic colon fermentation model suspended in a third water tank 73 and a third sampling system connected to the bionic colon fermentation model. The bionic colon fermentation model includes an ascending colon module, a transverse colon module, and a descending colon module with the same structure and connected in parallel. The ascending colon module includes an arc reactor 42a, a fifth cylindrical reactor 43a and a sixth cylindrical reactor 44a respectively connected to the two ends of the arc reactor 42a by flanges, the transverse colon module includes an arc reactor 42b, a fifth cylindrical reactor 43b and a sixth cylindrical reactor 44b respectively connected to the two ends of the arc reactor 42b by flanges, the descending colon module includes an arc reactor 42c, a fifth cylindrical reactor 43c and a sixth cylindrical reactor 44c respectively connected to the two ends of the arc reactor 42c by flanges, the fifth cylindrical reactor 43c and the sixth cylindrical reactor 44c each having three air outlets 50 and three air inlets 49 and are both connected to a ball valve 51, and each of the ball valves 51 is connected to a third temperature control system 64 and a PLC control system 58;

[0066] The first, second, and third water tanks 71, 72, and 73 all contain buoyant, transparent or translucent liquids with a density of 2.3-5.1 mol / L. The bionic small intestinal digestive system and the bionic colon fermentation system are respectively equipped with a second liquid level sensor 30 and a third liquid level sensor 63 for measuring the liquid level in the reaction liquid. The purpose is, firstly, that gas production during the reaction compresses the liquid surface, and the amount of gas production can be monitored in real time by the amount of liquid level drop. Secondly, a drop in the liquid level can expose the electrodes, preventing them from receiving signals effectively. The liquid level sensors can monitor the drop in the liquid level in real time to prevent the electrodes from losing their signals. The bionic gastrointestinal digestive system is controlled by a PLC.

[0067] The bionic stomach digestion system is connected to the second sample injection system via a first inter-system connecting pump 67 , and the bionic small intestine digestion system is connected to the third sample injection system via a second inter-system connecting pump 68 , and the third sample injection system is also connected to the bionic stomach digestion system.

[0068] Furthermore, the first sampling system includes a first peristaltic pump 15, a first sample bottle 18 and a first acid-base bottle 19 connected to the first peristaltic pump 15, and the first peristaltic pump 15 is connected to the intermediate 4 and the intermediate 5;

[0069] The second sampling system includes a second peristaltic pump 38, a second sample bottle 35 and a second acid and base bottle 36 connected to the second peristaltic pump 38, and the second peristaltic pump 38 is connected to the straight reactor 23;

[0070] The third sampling system includes a third peristaltic pump 53, a third sample bottle 54 connected to the third peristaltic pump, a third acid and alkali bottle 60, and a gas bottle 55; a solenoid valve 61 can also be added to the third sampling system to control the amount of gas introduced. The third peristaltic pump 53 is connected to the arc reactor 42;

[0071] like Figure 1 As shown, the first peristaltic pump 15 , the second peristaltic pump 38 , and the third peristaltic pump 53 are connected to each other and are all connected to the circulating air box.

[0072] Furthermore, the intermediate body 4 has a first sample inlet 11 and a first electrode port 12, and the intermediate body 5 has a first sample inlet 13 and a first electrode port 14. The first sample inlet 11 and the first sample inlet 13 are both connected to the first peristaltic pump 15, and the first electrode port 12 and the first electrode port 14 are both inserted with pH electrodes;

[0073] The straight reactor 23 has a second sample inlet 22, a second electrode port 28, a second microscope port 26, and a second tail gas analysis port 27. The second sample inlet 22 is connected to the second peristaltic pump 38. The second electrode port 28 is inserted with a pH electrode, and the second microscope port 26 is inserted with a microscope probe.

[0074] The arc reactor 42 has a third sampling port 47, a third electrode port 48, a third microscope port 46, and a third exhaust gas analysis port 45. The third sampling port 47 is connected to the third peristaltic pump 53. The third electrode port 48 is inserted with a pH electrode, the third microscope port 46 is inserted with a microscope probe, and the exhaust gas analysis port 45 is inserted with an exhaust gas analysis probe. The exhaust gas analysis probe can detect the component concentration of the mixed gas produced by the reaction in the arc reactor 42.

[0075] The pH electrode is used to measure the pH conditions in each reactor or intermediate body to coordinate with the feeding system for adjustment; the microscope probe is used to detect the digestion degree of the digestate at a certain time to better study the digestion conditions and observe the growth of bacteria in the colon reactor; the exhaust gas analysis probe can detect the component concentrations of the mixed gas produced by the reaction in the arc reactor, including but not limited to H2 concentration, CO2 concentration, NO concentration, CH4 concentration, H2S concentration, VOC concentration, NH3 concentration and other gases.

[0076] The gastric digestion model is equipped with a biomimetic silicone gastric tract 6 that bifurcates from the inner cavity of the spherical reactor 1 and extends into the inner cavities of the first and second cylindrical reactors 2 and 3. The biomimetic silicone gastric tract 6 extends from the inner cavities of the first and second cylindrical reactors 2 and 3 and is fixed to the outlets of the first and second cylindrical reactors 2 and 3, respectively. The outlets of the first and second cylindrical reactors 2 and 3 are located at the ends of the first and second cylindrical reactors 2 and 3 that are away from the intermediate body. The outlets of the first and second cylindrical reactors 2 and 3 are both connected to an adapter 8 and an output tube, each of which is connected to a Luer connector. The third and fourth cylindrical reactors 24 and 25 are equipped with a biomimetic silicone intestine 29, and the fifth and sixth cylindrical reactors 43 and 44 are equipped with a biomimetic silicone intestine 57. The biomimetic silicone gastric tract and biomimetic silicone intestine are elastic bag structures made of silicone material and can be turned outward and fixed to the outside of the flange. The Luer connector is provided to facilitate sampling.

[0077] Furthermore, the first water tank 71, the second water tank 72, and the third water tank 73 are respectively floating with a first buoy 20, a second buoy 39, and a third buoy 59, and the first buoy 20, the second buoy 39, and the third buoy 59 are respectively suspended with a bionic stomach digestive system, a bionic small intestine digestive system, and a bionic colon fermentation system.

[0078] The detection methods involved in the following embodiments are as follows:

[0079] Reducing sugar detection method:

[0080] DNS method determination;

[0081] Starch detection method:

[0082] Refer to the second method of acid hydrolysis method in GB / T5009.9-2008 "Determination of starch in foods";

[0083] Determination of metabolite short-chain fatty acids:

[0084] To 1 mL of fermentation broth, add 10 μl of 2-methylbutyric acid (1 mol L⁻¹) as an internal standard. Slowly add 250 μl of concentrated hydrochloric acid, mix thoroughly, add 1 mL of ether, vortex for 1 min, and allow the organic and aqueous phases to separate. Carefully remove the supernatant (organic phase), add anhydrous sodium sulfate, vortex, and filter through a 0.22 μm organic filter. 5 μl of the supernatant is then transferred to an Agilent 7890 gas chromatograph equipped with an electron capture detector (FID) (Agilent, USA) for determination of short-chain fatty acids as metabolites.

[0085] 16S rRNA content measurement method:

[0086] NanoDrop micro-volume spectrophotometer measurement method.

[0087] Buoyancy detection method:

[0088] F=ρg(V+SH)=GN

[0089] Where ρ is the liquid density, V is the reactor volume, S is the bottom area of ​​the cubic float 18, H is the depth of the cubic float 18 immersed in water, N is the gas impact force, with upward being the positive direction, and G is the total weight of the cubic float 18, the string, and the reaction device.

[0090] Given G = 1.5 kg, g = 9.81 N / kg, V = 1 m 3 , N=1.578N, the side length of the three-dimensional buoy is 0.328m.

[0091] When the density of salt water is 2 mol / L = 1.078 g / cm 3 When H=0.328, the buoy just completely enters the water surface, the machine sinks to the bottom and the gravity it is subjected to is 1 / 6 of its own weight.

[0092] When the density of salt water is 3 mol / L = 1.116 g / cm 3 When H=0, the machine sinks to the bottom and is subject to a gravity force of 1 / 6 of its own weight.

[0093] The present invention uses the above method to calculate the buoyancy given to the entire model by the liquid in the water tank to simulate the gravity exerted on the entire model in a weightless environment. The liquid density parameters provided by the present invention are only for simulating the weightless environment of the lunar surface in a vacuum (approximately 1 / 6 of the Earth's gravity), and do not simulate the gravity of non-celestial areas, space stations, space capsules, etc. in a vacuum.

[0094] Crushing capacity test method:

[0095] The stomach's crushing capacity was tested using three-color agar beads with varying compressive strengths. White beads (0.59N), black beads (0.72N), and red beads (0.93N) were placed into the stomach cavity. After peristalsis, the beads were removed and their crushing was observed. The crushing capacity of the stomach cavity was assessed based on the compressive strength and crushing behavior of the corresponding agar beads.

[0096] A. Bionic stomach digestive system:

[0097] Reference Example A: Gastric Weightlessness Rabbit Example

[0098] Step 1: Prepare food suspension

[0099] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0100] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 1g egg yolk, 1g table salt.

[0101] Step 2: Rabbit simulates weightlessness

[0102] Eighteen healthy rabbits were paired according to their weight and randomly divided into six groups for a 19-hour experimental period: three control groups were set up, which moved freely in the cage during the experiment; and nine experimental groups lived in a special head-down device to simulate the effect of weightlessness for 19 hours. After the experiment, valid data were collected from a total of 12 rabbits, including five in the control group and seven in the experimental group.

[0103] Animal experiment control: Rabbits were kept in a small hanging cage with their heads exposed, clamped tightly, and tilted at -20°. Their basic condition was observed and basic physiological data were collected.

[0104] Experimental Procedure Animal Diet: Feed the food suspension prepared in step 1 three times every 8 hours for a total of 16 hours.

[0105] Step 3: Dissection

[0106] After the last feeding, wait for the rabbit to digest for three hours and then dissect the rabbit immediately. All the digestive matter in the stomach is taken out and the reducing sugar concentration, fat concentration, protein concentration and degree of sample digestion are measured.

[0107] Example A1

[0108] Step 1: Prepare food suspension

[0109] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0110] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 1g egg yolk, 1g table salt.

[0111] Step 2: Preprocessing

[0112] Prepare a single reaction system of 250 mL of culture medium. Place the biomimetic stomach digestive system in a high-pressure steam autoclave and sterilize at 121°C for 20 minutes. After installing the sterilized biomimetic stomach digestive system, seal the reactor. Use a syringe to remove the air from the reactor. Use a valve to introduce an appropriate amount of nitrogen into the biomimetic stomach digestive system to maintain an anaerobic environment. Before adding the food sample, inject 250 mL of simulated gastric fluid to simulate the digestive environment within the stomach. Prepare saline solution with a concentration of 3 mol / L and pour it into the first water tank. Place the biomimetic stomach digestive system in the liquid and allow it to float, simulating weightlessness.

[0113] Step 3: Working process

[0114] (1) After the sterilized bionic gastric digestive system is installed, in order to more realistically simulate the process of ingesting corn flour, the food sample is added to the spherical reactor 1 from the simulated esophagus in batches. The spherical reactor 1 is used to simulate the gastric body module, and the first cylindrical reactor 2 and the second cylindrical reactor 3 are used to simulate the gastric fundus and gastric antrum modules respectively. The food sample is ingested from the first sampling port 11 and the first sampling port 13, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated gastric fluid is accurately controlled by the first peristaltic pump 15. Before the sampling, the pH between the gastric fundus and the gastric body is 1.0, and the pH between the gastric body and the gastric antrum is 1.5. After the sampling is completed, the first feed port 11 is closed to simulate the digestion process of food during emptying. After the sampling is completed, the first peristaltic pump 15 for controlling the acid and base is adjusted so that the pH between the gastric fundus and the gastric body is 2.0, and the pH between the gastric body and the gastric antrum is 3.0. The gastric device is peristaltically compressed by the peristaltic pump 10 of the first temperature control system. The compression method is as follows: the air inlet 16 is randomly air-intaken by a program and the air outlet 17 is randomly air-outletted to simulate the irregular peristalsis of the stomach. The peristaltic compression frequency of the gastric fundus and gastric antrum is 4 times / minute, the flow rate is 3L / min, the air filling volume is 750mL, and the compression amplitude is 3cm. The peristaltic compression frequency of the gastric body is 4 times / minute, the flow rate is 3L / min, the air filling volume is 1L, and the compression amplitude is 4cm.

[0115] (2) Regulate the peristaltic pump 10 and ball valve 9 of the first temperature control system to pump N2 to empty the third cylindrical reactor 3, thereby simulating the flattening and emptying of the gastric antrum module. The sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valves 9a, 9c, and 9d, and close ball valve 9b, so that the sample contained in the silicone bionic gastric tract 7 in the spherical reactor 1 is squeezed into the bionic stomach in the first cylindrical reactor 2.

[0116] (3) The gastric antrum remains flattened, the ball valves 9a, 9b, and 9c are opened, and the ball valve 9d is closed, so that the sample contained in the first cylindrical reactor 2 is squeezed into the bionic stomach in the spherical reactor 1.

[0117] (4) Repeat steps (2) and (3) to allow the gastric fundus and gastric body modules to peristalsize and empty during peristalsis.

[0118] (5) Open ball valve 9f and pump out N2 to relax the antrum module. Open ball valve 9c and close ball valve 9d to flatten and empty the fundus module. The sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valve 9a and close ball valve 9b. Under the circulating nitrogen pressure, the bionic stomach in the spherical reactor 1 is squeezed and the sample contained therein is squeezed into the bionic stomach in the second cylindrical reactor 3.

[0119] (6) The fundus of the stomach remains flattened, the ball valves 9a, 9b, and 9e are opened, and the ball valve 9f is closed, so that the sample contained in the second cylindrical reactor 3 is squeezed into the bionic stomach in the spherical reactor 1.

[0120] (7) Repeat steps (5) and (6) to allow the gastric body and gastric antrum modules to peristalsis and emptying to be achieved during peristalsis.

[0121] (8) After the food samples were digested for 3 hours, all the digested food in the stomach was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of digestion of the sample were measured. Figure 8-11 shown.

[0122] Example A2:

[0123] Step 1: Prepare food suspension

[0124] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0125] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 1g egg yolk, 1g table salt.

[0126] Step 2: Preprocessing

[0127] Prepare a single reaction system of 250 mL of culture medium. Place the biomimetic stomach digestive system in a high-pressure steam autoclave and sterilize at 121°C for 20 minutes. After installing the sterilized biomimetic stomach digestive system, seal the reactor. Use a syringe to remove the air from the reactor and introduce an appropriate amount of nitrogen into the system through a valve to maintain an oxygen-free environment. Before adding the food sample, inject 250 mL of simulated gastric fluid to simulate the digestive environment in the stomach. Prepare saline with a concentration of 3 mol / L and pour it into the first water tank. Place the biomimetic stomach digestive system in the liquid and allow it to float to simulate weightlessness.

[0128] Step 3: Working process

[0129] (1) After the sterilized bionic gastric digestive system is installed, in order to more realistically simulate the process of ingesting corn flour, food samples are added to the fundus module from the simulated esophagus in batches. The food samples are ingested from the first feed port 11, and the injection time lasts for 10 minutes. During the injection period, the input rate of the simulated gastric fluid is accurately controlled by the first peristaltic pump 15. Before the injection, the pH between the fundus and the body is 1.5, and the pH between the body and the antrum is 2.0. After the injection is completed, the first feed port 11 is closed to simulate the digestion process of food during emptying. After the addition of the sample, the first peristaltic pump 15 for controlling the acid and alkali is adjusted so that the pH between the fundus and the body is 3.0, and the pH between the body and the antrum is 4.0. The gastric device is peristaltically compressed by the peristaltic pump 10 of the first temperature control system. The compression method is as follows: the air inlet 16 is randomly air-intaken by a program and the air outlet 17 is randomly air-outletted to simulate the irregular peristalsis of the stomach. The peristaltic compression frequency of the gastric fundus and gastric antrum is 3 times / minute, the flow rate is 3L / min, the air filling volume is 750mL, and the compression amplitude is 3cm. The peristaltic compression frequency of the gastric body is 3 times / minute, the flow rate is 3L / min, the air filling volume is 1L, and the compression amplitude is 4cm.

[0130] (2) Open the peristaltic pump 10 and ball valve 9e of the first constant temperature control system, close ball valve 9f, and pump in N2 to flatten and empty the gastric antrum module, so that the sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valves 9a, 9c, and 9d, and close ball valve 9b, so that the sample contained in the silicone bionic gastric tract 7 in the spherical reactor 1 is squeezed into the bionic stomach in the first cylindrical reactor 2.

[0131] (3) The gastric antrum remains flattened, the ball valves 9a, 9b, and 9c are opened, and the ball valve 9d is closed, so that the sample contained in the first cylindrical reactor 2 is squeezed into the bionic stomach in the spherical reactor 1.

[0132] (4) Repeat steps (2) and (3) to allow the gastric fundus and gastric body modules to peristalsize and empty during peristalsis.

[0133] (5) Open ball valve 9f and pump out N2 to relax the antrum module. Open ball valve 9c and close ball valve 9d to flatten and empty the fundus module. The sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valve 9a and close ball valve 9b. Under the circulating nitrogen pressure, the bionic stomach in the spherical reactor 1 is squeezed and the sample contained therein is squeezed into the bionic stomach in the second cylindrical reactor 3.

[0134] (6) The fundus of the stomach remains flattened, the ball valves 9a, 9b, and 9e are opened, and the ball valve 9f is closed, so that the sample contained in the second cylindrical reactor 3 is squeezed into the bionic stomach in the spherical reactor 1.

[0135] (7) Repeat steps (5) and (6) to allow the gastric body and gastric antrum modules to peristalsis and emptying to be achieved during peristalsis.

[0136] (8) After the food samples were digested for 3 hours, all the digested food in the stomach was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of digestion of the sample were measured. Figure 8-11 shown.

[0137] Example A3:

[0138] Step 1: Prepare food suspension

[0139] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0140] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 1g egg yolk, 1g table salt.

[0141] Step 2: Preprocessing

[0142] Prepare a single reaction system of 250 mL of culture medium. Place the biomimetic stomach digestive system in a high-pressure steam autoclave and sterilize at 121°C for 20 minutes. After installing the sterilized biomimetic stomach digestive system, seal the reactor. Use a syringe to remove the air from the reactor. Use a valve to introduce an appropriate amount of nitrogen into the biomimetic stomach digestive system to maintain an anaerobic environment. Before adding the food sample, inject 250 mL of simulated gastric fluid to simulate the digestive environment within the stomach. Prepare saline solution with a concentration of 3 mol / L and pour it into the first water tank. Place the biomimetic stomach digestive system in the liquid and allow it to float, simulating weightlessness.

[0143] Step 3: Working process

[0144] (1) After the sterilized bionic gastric digestive system is installed, in order to more realistically simulate the process of ingesting corn flour, food samples are added to the fundus module from the simulated esophagus in batches. The food samples are ingested from the first feed port 11, and the injection time lasts for 10 minutes. During the injection period, the input rate of the simulated gastric fluid is accurately controlled by the first peristaltic pump 15. Before the injection, the pH between the fundus and the body is 1.0, and the pH between the body and the antrum is 1.5. After the injection is completed, the first feed port 11 is closed to simulate the digestion process of food during emptying. After the addition of the sample, the first peristaltic pump 15 for controlling the acid and alkali is adjusted so that the pH between the fundus and the body is 2.0, and the pH between the body and the antrum is 3.0. The stomach is peristaltically compressed by the peristaltic pump 10 of the first temperature control system. The compression method is: the air inlet 16 is randomly air-intaken and the air outlet 17 is randomly air-outletted through the program to simulate the irregular peristalsis of the stomach. The peristaltic compression frequency of the fundus and antrum is 4 times / minute, the flow rate is 3L / min, the air inflation volume is 500mL-1L, and the compression amplitude is 2cm. The peristaltic compression frequency of the gastric body is 4 times / minute, the flow rate is 3L / min, the air inflation volume is 1L, and the compression amplitude is 3cm.

[0145] (2) Open the peristaltic pump 10 and ball valve 9e of the first temperature control system, close ball valve 9f, and pump in N2 to flatten and empty the gastric antrum module, so that the sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valves 9a, 9c, and 9d, and close ball valve 9b, so that the sample contained in the silicone bionic gastric tract 7 in the spherical reactor 1 is squeezed into the bionic stomach in the first cylindrical reactor 2.

[0146] (3) The gastric antrum remains flattened, the ball valves 9a, 9b, and 9c are opened, and the ball valve 9d is closed, so that the sample contained in the first cylindrical reactor 2 is squeezed into the bionic stomach in the spherical reactor 1.

[0147] (4) Repeat steps (2) and (3) to allow the gastric fundus and gastric body modules to peristalsize and empty during peristalsis.

[0148] (5) Open ball valve 9f and pump out N2 to relax the antrum module. Open ball valve 9c and close ball valve 9d to flatten and empty the fundus module. The sample contained therein is squeezed into the bionic stomach in the spherical reactor 1. Open ball valve 9a and close ball valve 9b. Under the circulating nitrogen pressure, the bionic stomach in the spherical reactor 1 is squeezed and the sample contained therein is squeezed into the bionic stomach in the second cylindrical reactor 3.

[0149] (6) The fundus of the stomach remains flattened, the ball valves 9a, 9b, and 9e are opened, and the ball valve 9f is closed, so that the sample contained in the second cylindrical reactor 3 is squeezed into the bionic stomach in the spherical reactor 1.

[0150] (7) Repeat steps (5) and (6) to allow the gastric body and gastric antrum modules to peristalsis and emptying to be achieved during peristalsis.

[0151] (8) After the food samples were digested for 3 hours, all the digested food in the stomach was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of digestion of the sample were measured. Figure 8-10 shown.

[0152] Conclusion: Based on Examples A1-3 and the attached Figure 8-11 It can be seen that the bionic stomach digestive system of the present invention can achieve reducing sugar digestibility, protein digestibility, fat digestibility, and crushing capacity similar to those of a real stomach.

[0153] Comparative Example A1

[0154] The difference from Example A1 is that after the sample addition is completed, the first peristaltic pump 15 is adjusted and controlled to make the pH between the fundus and the body of the stomach 1.0, and the pH between the body of the stomach and the antrum of the stomach 2.0. The remaining steps and parameters are the same, resulting in a significant increase in the digestibility rate due to excessive acidity exceeding the actual value.

[0155] Comparative Example A2

[0156] The difference from Example A1 is that after the sample addition is completed, the first peristaltic pump 15 is adjusted and controlled to make the pH between the fundus and the body of the stomach 4.0, and the pH between the body of the stomach and the antrum of the stomach 5.0. The remaining steps and parameters are the same, resulting in an unsatisfactory digestion rate due to insufficient acidity.

[0157] Comparative Example A3

[0158] The difference from Example A1 is that there are no steps (5) (6) (7), and the other steps and parameters are the same. It is tested without the antrum module, and the result is that the digestibility is greatly reduced.

[0159] Comparative Example A4

[0160] The difference from Example A1 is that there are no steps (2)(3)(4)(5)(6)(7), and the other steps and parameters are the same. There is no gastric body and gastric antrum module, resulting in a greatly reduced digestibility.

[0161] Comparative Example A5

[0162] The difference from Example A2 is that the peristaltic compression frequency of the gastric fundus and antrum is 1 time / minute, and the peristaltic compression frequency of the gastric body is 1 time / minute. The other steps and parameters are the same. It is concluded that the low digestibility is caused by poor peristaltic effect.

[0163] Comparative Example A6

[0164] The difference from Example A2 is that the peristaltic compression frequency of the gastric fundus and antrum is 6 times / minute, and the peristaltic compression frequency of the gastric body is 6 times / minute. The other steps and parameters are the same. Although the digestion effect is good, the bionic digestive membrane is easily damaged and broken due to the excessive peristaltic frequency.

[0165] Comparative Example A7

[0166] The difference from Example A3 is that the air volume of the gastric fundus and gastric antrum is 250 mL, the compression amplitude is 1 cm, and the air volume of the gastric body is 500 mL, the compression amplitude is 1 cm. The other steps and parameters are the same, and the conclusion is that the peristaltic effect is poor.

[0167] Comparative Example A8

[0168] The difference from Example A3 is that the air inflation volume of the gastric fundus and gastric antrum is 1.25L, the compression amplitude is 5cm, and the air inflation volume of the gastric body is 1.5L, the compression amplitude is 5cm. The other steps and parameters are the same. However, due to the excessive peristaltic amplitude, the bionic digestive membrane is easily damaged and broken.

[0169] Comparative Example A9

[0170] The difference from Example A1 is that salt water with a concentration of 4 mol / L is prepared and poured into the simulated weightlessness water tank. The other steps and parameters are the same, and it is concluded that it is impossible to achieve the suspension of the device to simulate the weightlessness environment.

[0171] Comparative Example A10

[0172] The difference from Example A1 is that salt water with a concentration of 2 mol / L is prepared and poured into the simulated weightlessness water tank. The other steps and parameters are the same, and it is concluded that it is impossible to achieve suspension of the device to simulate a weightless environment.

[0173] Comparative Example A11

[0174] Using a traditional device structure with only one air inlet and outlet for simulation, the irregular peristalsis in the first step cannot be achieved, and it can only compress the entire bionic silicone intestine.

[0175] Comparative Example A12

[0176] When the conventional device structure without intermediate 4 is used for simulation, the pH test between the fundus and the body of the stomach in the first step cannot be realized, and the pH in the stomach cannot be simulated more realistically.

[0177] B. Bionic small intestine digestive system:

[0178] Reference Example B: Small Intestine Weightlessness Rabbit Example

[0179] Step 1: Prepare food suspension

[0180] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0181] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0182] Step 2: Rabbit simulates weightlessness

[0183] 18 healthy rabbits were paired by weight and randomly divided into 6 groups. The experimental period was 19 hours: 3 groups were set up as control groups, which moved freely in the cage during the experiment; 9 groups were set up as experimental groups, which lived in a special head-down weightlessness device for 19 hours. After the experiment, valid data were collected from 12 rabbits, including 5 in the control group and 7 in the experimental group.

[0184] Animal experiment control: The rabbits were kept in a small hanging cage with their heads exposed, clamped tightly, and their heads lowered by -20 degrees. The rabbits were kept in the animal cage to observe their basic condition and collect basic physiological data.

[0185] Experimental Procedure Animal Diet: Feed the food suspension prepared in step 1 three times every 8 hours for a total of 16 hours.

[0186] Step 3: Dissection

[0187] After the last feeding, wait for the rabbit to digest for three hours and then dissect the rabbit immediately. All the digestive matter in the intestine is taken out and the reducing sugar concentration, protein concentration and degree of sample digestion are measured.

[0188] Example B1

[0189] Step 1: Prepare food suspension

[0190] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0191] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0192] Step 2: Preprocessing

[0193] Place the biomimetic small intestinal digestive system in an autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic small intestinal digestive system, seal the reactor and remove the air with a syringe, maintaining the liquid level above or below the scale. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment within the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into a second water tank. Place the device in the liquid and allow it to float, simulating weightlessness.

[0194] Step 3: Working process

[0195] (1) After the sterilized bionic small intestine digestive system is installed, in order to more realistically simulate the process of food intake, food samples are added from the simulated esophagus to the duodenum module in batches. The food samples are taken in from the second sampling port 22, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the second peristaltic pump 38. After the sampling of the duodenum module is completed, the second sampling port 22 is closed to simulate the digestion process of food during emptying. After the addition is completed, the second peristaltic pump 38 is adjusted to control the pH in the straight reactor 23 to 5.5, simulating the acid-base environment of the duodenum in the body. The intestine is peristaltically compressed by the second temperature control system peristaltic pump 37, with a peristaltic compression frequency of 6 times / minute, a flow rate of 3L / min, an air inflation volume of 750mL, and a compression amplitude of 3cm.

[0196] (2) Open the ball valve 34 of the third cylindrical reactor 24a and close the ball valve 34 of the fourth cylindrical reactor 25a, allowing the biomimetic silicone intestine 29 in the third cylindrical reactor 24a of the biomimetic small intestinal digestive system to begin peristalsis and achieve emptying during peristalsis. Under the circulating pressure, the biomimetic silicone intestine in the third cylindrical reactor 24a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine in the fourth cylindrical reactor 25a. Operation is performed using any air inlet / ball valve on the third and fourth cylindrical reactors.

[0197] (3) Similarly, the ball valve 34 of the fourth cylindrical reactor 25a is opened and the ball valve 34 of the third cylindrical reactor 24a is closed; under the circulating pressure, the bionic intestine in the fourth cylindrical reactor 25a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the third cylindrical reactor 25a.

[0198] (4) The chyme in the duodenum module is pumped into the jejunum module through the first intestinal connecting pump 66 at a chyme transport rate of 15 mL / min. The second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 6.5.

[0199] (5) For the jejunum module, repeat steps (2) and (3).

[0200] (6) The chyme in the jejunum module is pumped into the ileum module through the first intestinal connecting pump 66, and the chyme transport rate is 45 mL / min. The switch of the second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 7.5.

[0201] (7) For the ileum module, repeat steps (2) and (3).

[0202] (8) After the food samples were digested for 3 hours, all the digestive matter in the intestine was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of sample digestion were measured. Figure 8-11 shown.

[0203] Example B2

[0204] Step 1: Prepare food suspension

[0205] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0206] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0207] Step 2: Preprocessing

[0208] Place the biomimetic small intestinal digestive system in an autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic small intestinal digestive system, seal the reactor and remove the air with a syringe, maintaining the liquid level above or below the scale. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment within the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into a second water tank. Place the device in the liquid and allow it to float, simulating weightlessness.

[0209] Step 3: Working process

[0210] (1) After the sterilized bionic small intestine digestive system is installed, in order to more realistically simulate the process of food intake, food samples are added from the simulated esophagus to the duodenum module in batches. The food samples are taken in from the second sampling port 22, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the second peristaltic pump 38. After the sampling of the duodenum module is completed, the second sampling port 22 is closed to simulate the digestion process of food during emptying. After the addition of samples is completed, the second peristaltic pump 38 is adjusted to control the pH in the straight reactor 23 to 5.5, simulating the acid-base environment of the duodenum in the body. The intestine is peristaltically compressed by the second temperature control system peristaltic pump 37, with a peristaltic compression frequency of 5 times / minute, a flow rate of 3L / min, an air inflation volume of 750mL, and a compression amplitude of 3cm.

[0211] (2) Open the ball valve 34 of the third cylindrical reactor 24a and close the ball valve 34 of the fourth cylindrical reactor 25a, allowing the biomimetic silicone intestine 29 in the third cylindrical reactor 24a of the biomimetic small intestinal digestive system to begin peristalsis and achieve emptying during peristalsis. Under the circulating nitrogen pressure, the biomimetic silicone intestine in the third cylindrical reactor 24a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine in the fourth cylindrical reactor 25a. Operation is performed using any air inlet / ball valve on the third and fourth cylindrical reactors.

[0212] (3) Similarly, the ball valve 34 of the fourth cylindrical reactor 25a is opened and the ball valve 34 of the third cylindrical reactor 24a is closed. Under the circulating pressure, the bionic intestine in the fourth cylindrical reactor 25a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the third cylindrical reactor 25a.

[0213] (4) The chyme in the duodenum module is pumped into the jejunum module through the first intestinal connecting pump 66 at a chyme transport rate of 15 mL / min. The second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 6.5.

[0214] (5) For the jejunum module, repeat steps (2) and (3).

[0215] (6) The chyme in the jejunum module is pumped into the ileum module through the first intestinal connecting pump 66, and the chyme transport rate is 45 mL / min. The switch of the second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 7.5.

[0216] (7) For the ileum module, repeat steps (2) and (3).

[0217] (8) After the food samples were digested for 3 hours, all the digestive matter in the intestine was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of sample digestion were measured. Figure 8-11 shown.

[0218] Example B3

[0219] Step 1: Prepare food suspension

[0220] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0221] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0222] Step 2: Preprocessing

[0223] Place the biomimetic small intestinal digestive system in an autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic small intestinal digestive system, seal the reactor and remove the air with a syringe, maintaining the liquid level above or below the scale. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment within the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into a second water tank. Place the device in the liquid and allow it to float, simulating weightlessness.

[0224] Step 3: Working process

[0225] (1) After the sterilized bionic small intestine digestive system is installed, in order to more realistically simulate the process of food intake, food samples are added from the simulated esophagus to the duodenum module in batches. The food samples are taken in from the second sampling port 22, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the second peristaltic pump 38. After the sampling of the duodenum module is completed, the second sampling port 22 is closed to simulate the digestion process of food during emptying. After the addition of samples is completed, the second peristaltic pump 38 is adjusted to control the pH in the straight reactor 23 to 5.5, simulating the acid-base environment of the duodenum in the body. The intestine is peristaltically compressed by the second temperature control system peristaltic pump 37, with a peristaltic compression frequency of 4 times / minute, a flow rate of 3L / min, an air inflation volume of 1L, and a compression amplitude of 4cm.

[0226] (2) Open the ball valve 34 of the third cylindrical reactor 24a and close the ball valve 34 of the fourth cylindrical reactor 25a, allowing the biomimetic silicone intestine 29 in the third cylindrical reactor 24a of the biomimetic small intestinal digestive system to begin peristalsis and achieve emptying during peristalsis. Under the circulating nitrogen pressure, the biomimetic silicone intestine in the third cylindrical reactor 24a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine in the fourth cylindrical reactor 25a. Operation is performed using any air inlet / ball valve on the third and fourth cylindrical reactors.

[0227] (3) Similarly, the ball valve 34 of the fourth cylindrical reactor 25a is opened and the ball valve 34 of the third cylindrical reactor 24a is closed. Under the circulating pressure, the bionic intestine in the fourth cylindrical reactor 25a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the third cylindrical reactor 25a.

[0228] (4) The chyme in the duodenum module is pumped into the jejunum module through the first intestinal connecting pump 66 at a chyme transport rate of 15 mL / min. The second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 6.5.

[0229] (5) For the jejunum module, repeat steps (2) and (3).

[0230] (6) The chyme in the jejunum module is pumped into the ileum module through the first intestinal connecting pump 66, and the chyme transport rate is 45 mL / min. The switch of the second peristaltic pump 38 is adjusted to control the pH in the straight reactor at 7.5.

[0231] (7) For the ileum module, repeat steps (2) and (3).

[0232] (8) After the food samples were digested for 3 hours, all the digestive matter in the intestine was taken out and the reducing sugar concentration, protein concentration, fat concentration and degree of sample digestion were measured. Figure 8-11 shown.

[0233] Conclusion: Based on the examples B1-3 and the attached Figure 8-11 It can be seen that the bionic small intestine digestive system of the present invention can achieve reducing sugar digestibility, protein digestibility, fat digestibility, and crushing capacity similar to those of a real stomach.

[0234] Comparative Example B1

[0235] The difference from Example B1 is that the pH in the straight reactor 23a is controlled at 3, the pH in the straight reactor 23b is controlled at 4, and the pH in the straight reactor 23c is controlled at 5. The other steps and parameters are the same, resulting in a significant increase in the peracid digestibility exceeding the actual value.

[0236] Comparative Example B2

[0237] The difference from Example B1 is that the pH in the straight reactor 23a is controlled at 6.5, the pH in the straight reactor 23b is controlled at 7, and the pH in the straight reactor 23c is controlled at 8.5. The remaining steps and parameters are the same, resulting in unsatisfactory digestibility due to insufficient acidity.

[0238] Comparative Example B3

[0239] The difference from Example B1 is that there is no step (7), and the other steps and parameters are the same. There is no ileum module, and the result is that the digestibility is greatly reduced.

[0240] Comparative Example B4

[0241] The difference from Example B1 is that there are no steps (5) (6) (7). The other steps and parameters are the same. There is no ileum module. As a result, the digestibility is greatly reduced.

[0242] Comparative Example B5

[0243] The difference from Example B2 is that the peristaltic compression frequency of each module of the small intestine is 2 times / minute, and the other steps and parameters are the same. It is concluded that the low digestibility is caused by poor peristaltic effect.

[0244] Comparative Example B6

[0245] The difference from Example B2 is that the peristaltic compression frequency of each small intestine module is 8 times / minute, and the other steps and parameters are the same. Although the digestion effect is good, the bionic digestive membrane is easily damaged and broken due to the excessive peristaltic frequency.

[0246] Comparative Example B7

[0247] The difference from Example B3 is that the air volume of each small intestine module is 300 mL and the compression amplitude is 1 cm. The other steps and parameters are the same.

[0248] The conclusion is that the peristalsis effect is not good.

[0249] Comparative Example B8

[0250] The difference from Example B3 is that the air volume of each small intestine module is 1.25L and the compression amplitude is 5cm. The other steps and parameters are the same.

[0251] However, due to the large amplitude of peristalsis, the bionic digestive membrane is easily damaged and broken.

[0252] Comparative Example B9

[0253] Using a traditional device structure with only one air inlet and outlet for simulation, the irregular peristalsis in the first step cannot be achieved, and it can only compress the entire bionic silicone intestine.

[0254] C. Bionic small intestine digestive system:

[0255] Reference Example C: Colonic Weightlessness Rabbit Example

[0256] Step 1: Prepare food suspension

[0257] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0258] Food powder formula: 100g amylose.

[0259] Step 2: Rabbit simulates weightlessness

[0260] 18 healthy rabbits were paired by weight and randomly divided into 6 groups. The experimental period was 19 hours: 3 groups were set up as control groups, which moved freely in the cage during the experiment; 9 groups were set up as experimental groups, which lived in a special head-down weightlessness device for 19 hours. After the experiment, valid data were collected from 12 rabbits, including 5 in the control group and 7 in the experimental group.

[0261] Animal experiment control: The rabbits were kept in a small hanging cage with their heads exposed, clamped tightly, and their heads lowered by -20 degrees. The rabbits were kept in the animal cage to observe their basic condition and collect basic physiological data.

[0262] Experimental Procedure Animal Diet: Feed the food suspension prepared in step 1 three times every 8 hours for a total of 16 hours.

[0263] Step 3: Dissection

[0264] After the last feeding, wait for the rabbit to digest for three hours and then dissect the rabbit immediately. All the digestive materials in the intestine are taken out and the reducing sugar concentration, 16s-RNA, short-chain fatty acid content, intestinal gas production and sample digestion degree are measured.

[0265] Example C1:

[0266] Step 1: Prepare food suspension

[0267] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0268] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0269] Step 2: Preprocessing

[0270] Prepare a single reaction system of 250 mL of culture medium. Place the prepared culture medium and the biomimetic colon fermentation system in a high-pressure steam autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic colon fermentation system, draw the culture medium into the reactor via a third peristaltic pump, leaving a vent when adding the culture medium. Then, inject 10 mL of the prepared intestinal microbial sample into the fifth cylindrical reactor 43 using a 10 mL medical syringe via a Luer connector. After addition, seal the fifth cylindrical reactor 43, remove the air from the reactor using a syringe, and maintain an anaerobic environment by introducing an appropriate amount of nitrogen into the reactor 43, keeping the liquid level above and below the scale mark. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment in the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into the third water tank. Place the biomimetic colon fermentation system in the saline solution and allow it to float, simulating weightlessness.

[0271] Step 3: Working process

[0272] (1) After the sterilized bionic colon fermentation system is installed, in order to more realistically simulate the process of ingesting corn flour, the food samples are added from the simulated esophagus to the ascending colon module in batches. The food samples are ingested from the sampling port 6, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the third peristaltic pump 53. After the sampling of the ascending colon module is completed, the third sampling port 47 is closed to simulate the digestion process of food during emptying. After the addition is completed, the third peristaltic pump 53 is adjusted and controlled to control the pH in the arc reactor to 6.2, simulating the acid-base environment of the colon in the body. The intestine is peristaltically compressed by the third temperature control system peristaltic pump 56, with a peristaltic compression frequency of 4 times / minute, a flow rate of 3L / min, an air inflation volume of 750mL, and a compression amplitude of 3cm.

[0273] (2) Open the ball valve 51 on the sixth cylindrical reactor 44a and close the ball valve 51 on the fifth cylindrical reactor 43a, allowing the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a to begin peristalsis and achieve emptying during peristalsis. Under the circulating nitrogen pressure, the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine 57 in the sixth cylindrical reactor 44a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0274] (3) Similarly, open the ball valve 51 on the fifth cylindrical reactor 43a and close the ball valve 51 on the sixth cylindrical reactor 44a. Under the circulating nitrogen pressure, the bionic intestine in the sixth cylindrical reactor 44a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the fifth cylindrical reactor 43a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0275] (4) The chyme in the ascending colon module is pumped into the transverse colon module through the second intestinal connecting pump 65, with a chyme transport rate of 15 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 6.8.

[0276] (5) For the transverse colon module, repeat steps (2) and (3).

[0277] (6) The chyme in the transverse colon module is pumped into the descending colon module through the second intestinal connecting pump 65, with a chyme transport rate of 45 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 7.2.

[0278] (7) For the descending colon module, repeat steps (2) and (3).

[0279] (8) After the food samples were digested for 3 h, all the digestive matter in the intestine was taken out and the reducing sugar concentration, 16s-RNA, short-chain fatty acid content, intestinal gas production and sample digestion degree were measured. Figure 8-11 shown.

[0280] Example C2:

[0281] Step 1: Prepare food suspension

[0282] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0283] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0284] Step 2: Preprocessing

[0285] Prepare a single reaction system of 250 mL of culture medium. Place the prepared culture medium and the biomimetic colon fermentation system in a high-pressure steam autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic colon fermentation system, draw the culture medium into the reactor via a third peristaltic pump, leaving a vent when adding the culture medium. Then, inject 10 mL of the prepared intestinal microbial sample into the fifth cylindrical reactor 43 using a 10 mL medical syringe via a Luer connector. After addition, seal the fifth cylindrical reactor 43, remove the air from the reactor using a syringe, and maintain an anaerobic environment by introducing an appropriate amount of nitrogen into the reactor 43, keeping the liquid level above and below the scale mark. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment in the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into the third water tank. Place the biomimetic colon fermentation system in the saline solution and allow it to float, simulating weightlessness.

[0286] Step 3: Working process

[0287] (1) After the sterilized bionic colon fermentation system is installed, in order to more realistically simulate the process of ingesting corn flour, the food samples are added from the simulated esophagus to the ascending colon module in batches. The food samples are ingested from the sampling port 6, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the third peristaltic pump 53. After the sampling of the ascending colon module is completed, the third sampling port 47 is closed to simulate the digestion process of food during emptying. After the sampling is completed, the third peristaltic pump 53 is adjusted and controlled to control the pH in the arc reactor to 6.2, simulating the acid-base environment of the colon in the body. The intestine is peristaltically compressed by the third temperature control system peristaltic pump 56, with a peristaltic compression frequency of 3 times / minute, a flow rate of 3L / min, an air inflation volume of 750mL, and a compression amplitude of 3cm.

[0288] (2) Open the ball valve 51 on the sixth cylindrical reactor 44a and close the ball valve 51 on the fifth cylindrical reactor 43a, allowing the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a to begin peristalsis and achieve emptying during peristalsis. Under the circulating nitrogen pressure, the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine 57 in the sixth cylindrical reactor 44a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0289] (3) Similarly, open the ball valve 51 on the fifth cylindrical reactor 43a and close the ball valve 51 on the sixth cylindrical reactor 44a. Under the circulating nitrogen pressure, the bionic intestine in the sixth cylindrical reactor 44a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the fifth cylindrical reactor 43a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0290] (4) The chyme in the ascending colon module is pumped into the transverse colon module through the second intestinal connecting pump 65, with a chyme transport rate of 15 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 6.8.

[0291] (5) For the transverse colon module, repeat steps (2) and (3).

[0292] (6) The chyme in the transverse colon module is pumped into the descending colon module through the second intestinal connecting pump 65, with a chyme transport rate of 45 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 7.2.

[0293] (7) For the descending colon module, repeat steps (2) and (3).

[0294] (8) After the food samples were digested for 3 h, all the digestive matter in the intestine was taken out and the reducing sugar concentration, 16s-RNA, short-chain fatty acid content, intestinal gas production and sample digestion degree were measured. Figure 8-11 shown.

[0295] Example C3:

[0296] Step 1: Prepare food suspension

[0297] Weigh an appropriate amount of food powder and dilute it with 0.2% (w / v) guar gum solution to form a suspension.

[0298] Food powder formula: 100g amylose, 40g concentrated whey protein powder, 5g egg yolk.

[0299] Step 2: Preprocessing

[0300] Prepare a single reaction system of 250 mL of culture medium. Place the prepared culture medium and the biomimetic colon fermentation system in a high-pressure steam autoclave at 121°C for 20 minutes. After installing the sterilized biomimetic colon fermentation system, draw the culture medium into the reactor via a third peristaltic pump, leaving a vent when adding the culture medium. Then, inject 10 mL of the prepared intestinal microbial sample into the fifth cylindrical reactor 43 using a 10 mL medical syringe via a Luer connector. After addition, seal the fifth cylindrical reactor 43, remove the air from the reactor using a syringe, and maintain an anaerobic environment by introducing an appropriate amount of nitrogen into the reactor 43, keeping the liquid level above and below the scale mark. Before adding the food sample, inject 250 mL of simulated intestinal fluid to simulate the digestive environment in the intestine. Prepare saline solution with a concentration of 3 mol / L and pour it into the third water tank. Place the biomimetic colon fermentation system in the saline solution and allow it to float, simulating weightlessness.

[0301] Step 3: Working process

[0302] (1) After the sterilized bionic colon fermentation system is installed, in order to more realistically simulate the process of ingesting corn flour, the food samples are added from the simulated esophagus to the ascending colon module in batches. The food samples are ingested from the sampling port 6, and the sampling time lasts for 10 minutes. During the sampling period, the input rate of the simulated intestinal fluid is accurately controlled by the third peristaltic pump 53. After the sampling of the ascending colon module is completed, the third sampling port 47 is closed to simulate the digestion process of food during emptying. After the addition of samples is completed, the third peristaltic pump 53 is adjusted and controlled to control the pH in the arc reactor to 6.2, simulating the acid-base environment of the colon in the body. The intestine is peristaltically compressed by the third temperature control system peristaltic pump 56, with a peristaltic compression frequency of 4 times / minute, a flow rate of 3L / min, an air inflation volume of 750mL, and a compression amplitude of 2cm.

[0303] (2) Open the ball valve 51 on the sixth cylindrical reactor 44a and close the ball valve 51 on the fifth cylindrical reactor 43a, allowing the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a to begin peristalsis and achieve emptying during peristalsis. Under the circulating nitrogen pressure, the biomimetic silicone intestine 57 in the fifth cylindrical reactor 43a is squeezed, and the sample contained therein is squeezed into the biomimetic silicone intestine 57 in the sixth cylindrical reactor 44a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0304] (3) Similarly, open the ball valve 51 on the fifth cylindrical reactor 43a and close the ball valve 51 on the sixth cylindrical reactor 44a. Under the circulating nitrogen pressure, the bionic intestine in the sixth cylindrical reactor 44a is squeezed, and the sample contained therein is squeezed into the bionic intestine in the fifth cylindrical reactor 43a. Operation is performed using any of the air inlets / ball valves on the fifth and sixth cylindrical reactors.

[0305] (4) The chyme in the ascending colon module is pumped into the transverse colon module through the second intestinal connecting pump 65, with a chyme transport rate of 15 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 6.8.

[0306] (5) For the transverse colon module, repeat steps (2) and (3).

[0307] (6) The chyme in the transverse colon module is pumped into the descending colon module through the second intestinal connecting pump 65, with a chyme transport rate of 45 mL / min. The third peristaltic pump 53 is adjusted to control the pH in the reactor at 7.2.

[0308] (7) For the descending colon module, repeat steps (2) and (3).

[0309] (8) After the food samples were digested for 3 h, all the digestive matter in the intestine was taken out and the reducing sugar concentration, 16s-RNA, short-chain fatty acid content, intestinal gas production and sample digestion degree were measured. Figure 8-11 shown.

[0310] Conclusion: Based on the examples C1-3 and the attached Figure 8-11 It can be seen that the bionic colon fermentation system of the present invention can achieve reducing sugar digestibility, protein digestibility, fat digestibility, and crushing capacity similar to those of a real stomach.

[0311] Comparative Example C1

[0312] The difference from Example C1 is that the pH in the arc reactor 42a is controlled at 5, the pH in the arc reactor 42b is controlled at 5.5, and the pH in the arc reactor 42c is controlled at 6. The other steps and parameters are the same, resulting in some bacteria being unable to survive due to excessive acidity.

[0313] Comparative Example C2

[0314] The difference from Example C1 is that the pH in arc reactor 42a is controlled at 7, the pH in arc reactor 42b is controlled at 7.5, and the pH in arc reactor 42c is controlled at 8. The remaining steps and parameters are the same, resulting in unsatisfactory digestibility due to insufficient acidity.

[0315] Comparative Example C3

[0316] The difference from Example C1 is that there are no steps (6) and (7). The other steps and parameters are the same. There is no descending colon module. As a result, the digestibility is greatly reduced.

[0317] Comparative Example C4

[0318] The difference from Example C1 is that there are no steps (4) (5) (6) (7), and the other steps and parameters are the same. There is no transverse colon and descending colon module. As a result, the digestibility is greatly reduced.

[0319] Comparative Example C5

[0320] The difference from Example C2 is that the peristaltic compression frequency of each module of the small intestine is 2 times / minute, and the other steps and parameters are the same. It is concluded that the low digestibility is caused by poor peristaltic effect.

[0321] Comparative Example C6

[0322] The difference from Example C2 is that the peristaltic compression frequency of each small intestine module is 7 times / minute, and the other steps and parameters are the same. Although the digestion effect is good, the bionic digestive membrane is easily damaged and broken due to the excessive peristaltic frequency.

[0323] Comparative Example C7

[0324] The difference from Example C3 is that the air inflation volume of each small intestine module is 300 mL, the compression amplitude is 1 cm, and the other steps and parameters are the same, resulting in a conclusion that the peristaltic effect is not good.

[0325] Comparative Example C8

[0326] The difference from Example C3 is that the air inflation volume of each small intestine module is 1.25 L, the compression amplitude is 5 cm, and the other steps and parameters are the same. However, due to the excessive peristaltic amplitude, the bionic digestive membrane is easily damaged and broken.

[0327] Comparative Example C9

[0328] Using a traditional device structure with only one air inlet and outlet for simulation, the irregular peristalsis in the first step cannot be achieved, and it can only compress the entire bionic silicone intestine.

[0329] Although the present invention has been disclosed above in terms of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A bionic gastrointestinal digestive system in a lunar environment, characterized in that: Includes interconnected: A bionic gastric digestion system comprises a bionic gastric digestion model suspended in a first water tank and a first sampling system connected to the bionic gastric digestion model, wherein the bionic gastric digestion model comprises a spherical reactor, wherein the spherical reactor is connected to a symmetrically arranged first cylindrical reactor and a second cylindrical reactor via a flange and an intermediate body, wherein the spherical reactor, the first cylindrical reactor, and the second cylindrical reactor each have an air inlet and an air outlet, and each of the first cylindrical reactor and the second cylindrical reactor has at least three air inlets and at least three air outlets; A bionic small intestinal digestion system, comprising a bionic small intestinal digestion model suspended in a second water tank and a second sampling system connected to the bionic small intestinal digestion model, wherein the bionic small intestinal digestion model comprises a duodenum module, a jejunum module, and an ileum module of identical structure and connected in parallel, wherein the duodenum module comprises a straight reactor, and a third and fourth tubular reactors respectively connected to both ends of the straight reactor by flanges, wherein the third and fourth tubular reactors each have at least three air inlets and at least three air outlets; as well as A bionic colon fermentation system, comprising a bionic colon fermentation model suspended in a third water tank and a third sampling system connected to the bionic colon fermentation model. The bionic colon fermentation model comprises an ascending colon module, a transverse colon module, and a descending colon module having identical structures and connected in parallel. The ascending colon module comprises an arc reactor, and a fifth and sixth cylindrical reactors respectively connected to both ends of the arc reactor via flanges. The fifth and sixth cylindrical reactors each have at least three air inlets and at least three air outlets. Among them, the first sampling system, the second sampling system, and the third sampling system are all connected to the circulating air box to form a circulation; the first water tank, the second water tank, and the third water tank all have buoyant transparent or translucent liquid, and the density of the liquid is 2-3 mol / L; the bionic small intestine digestion system and the bionic colon fermentation system are both provided with liquid level sensors, and the bionic gastrointestinal digestion system is controlled by PLC; the bionic gastric digestion system is connected to the second sampling system by the first system connecting pump, and the bionic small intestine digestion system is connected to the third sampling system by the second system connecting pump, and the third sampling system is also connected to the bionic gastric digestion system.

2. The bionic gastrointestinal digestive system for lunar environment according to claim 1, characterized in that: The first sampling system includes a first peristaltic pump, a first sample bottle connected to the first peristaltic pump, and a first acid and base bottle, wherein the first peristaltic pump is connected to two intermediates; The second sampling system includes a second peristaltic pump, a second sample bottle and a second acid and base bottle connected to the second peristaltic pump, and the second peristaltic pump is connected to the straight reactor; The third sampling system includes a third peristaltic pump, a third sample bottle and a third acid and alkali bottle, and a gas bottle connected to the third peristaltic pump, and the third peristaltic pump is connected to the arc reactor; The first peristaltic pump, the second peristaltic pump, and the third peristaltic pump are connected to each other and are all connected to a circulating air box.

3. The bionic gastrointestinal digestive system for lunar environment according to claim 2, characterized in that: The intermediate has a first injection port and a first electrode port, the first injection port is connected to the first peristaltic pump, and the first electrode port is inserted with a pH electrode; The straight reactor has a second injection port, a second electrode port, a second microscope port, and a second tail gas analysis port, the second injection port and the second acid and alkali addition port are connected to the second peristaltic pump, the second electrode port is inserted with a pH electrode, and the second microscope port is inserted with a microscope probe; The arc-shaped reactor has a third sampling port, a third electrode port, a third microscope port, and a third exhaust gas analysis port. The third sampling port is connected to the third peristaltic pump, the third electrode port is inserted with a pH electrode, the third microscope port is inserted with a microscope probe, and the exhaust gas analysis port is inserted with an exhaust gas analysis probe. The exhaust gas analysis probe can detect the component concentration of the mixed gas generated by the reaction in the arc-shaped reactor.

4. The bionic gastrointestinal digestive system for lunar environment according to claim 3, characterized in that: The gastric digestion model is provided with a bionic silicone gastric tract that branches from the inner cavity of the spherical reactor and extends to the inner cavities of the first and second cylindrical reactors. The bionic silicone gastric tract extends from the inner cavities of the first and second cylindrical reactors and is fixed to the outlet of the first and second cylindrical reactors. The outlet of the first and second cylindrical reactors is located at the end of the first and second cylindrical reactors away from the intermediate body; the outlet of the first and second cylindrical reactors are both connected to an adapter and an output tube, and the output tube is connected to a Luer connector; the third, fourth, fifth and sixth cylindrical reactors are all provided with bionic silicone intestines.

5. The bionic gastrointestinal digestive system for lunar environment according to claim 3, characterized in that: The first cylindrical reactor and the second cylindrical reactor both have four air outlets and four air inlets, and at least one air outlet and at least one air inlet are connected to a ball valve, and each of the ball valves is connected to the first temperature control system and the PLC control system; the spherical reactor has at least three air inlets and each is connected to a ball valve.

6. The bionic gastrointestinal digestive system for lunar environment according to claim 3, characterized in that: The third cylindrical reactor and the fourth cylindrical reactor both have four gas outlets and four gas inlets and are both connected to ball valves, and each ball valve is connected to the second temperature control system and the PLC control system.

7. The bionic gastrointestinal digestive system for lunar environment according to claim 3, characterized in that: The fifth cylindrical reactor and the sixth cylindrical reactor both have three gas outlets and three gas inlets and are both connected to ball valves, and each ball valve is connected to the third temperature control system and the PLC control system.

8. The bionic gastrointestinal digestive system for lunar environment according to claim 1, characterized in that: The first water tank, the second water tank and the third water tank are respectively floated with a first buoy, a second buoy and a third buoy, and the first buoy, the second buoy and the third buoy are respectively suspended with a bionic stomach digestive system, a bionic small intestine digestive system and a bionic colon fermentation system.

9. The bionic gastrointestinal digestive system for lunar environment according to claim 1, characterized in that: Before feeding, the bionic stomach digestion system regulates the pH of the intermediate between the first cylindrical reactor and the spherical reactor to be within the range of 1.0-1.5 and the pH of the intermediate between the second cylindrical reactor and the spherical reactor to be within the range of 1.5-2.0 through the first feeding system; after feeding, the bionic stomach digestion system regulates the pH of the intermediate between the first cylindrical reactor and the spherical reactor to be within the range of 2.0-3.0 and the pH of the intermediate between the second cylindrical reactor and the spherical reactor to be within the range of 3.0-4.0 through the first feeding system; After feeding, the bionic small intestine digestion system regulates the pH of the duodenum module, the jejunum module, and the ileum module to be within the range of 5.2-5.8, 5.8-6.5, and 6.5-7.8 respectively through the second feeding system; After feeding, the bionic colon fermentation system regulates the pH of the transverse colon module, the descending colon module, and the ascending colon module to be within the range of 6.0-6.5, 6.5-7.0, and 7.0-7.5 respectively through the second feeding system.

10. The bionic gastrointestinal digestive system for lunar environment according to claim 1, characterized in that: The peristaltic compression frequency of the first and second cylindrical reactors is 3-5 times / min, the flow rate is 2-4 L / min, the air filling volume is 350-750 mL, and the compression amplitude is 1.5-3.5 cm; the peristaltic compression frequency of the spherical reactor is 3-4 times / min, the flow rate is 2-4 L / min, the air filling volume is 500 mL-1 L, and the compression amplitude is 2.5-4.5 cm; The flow rate between the duodenum module and the jejunum module is 15-25 mL / min, the flow rate between the jejunum module and the ileum module is 35-65 mL / min, the peristaltic compression frequency of the third cylindrical reactor and the fourth cylindrical reactor is 3-5 times / minute, the flow rate is 2-4 L / min, the air inflation volume is 350-750 mL, and the compression amplitude is 1.5-3.5 cm; The flow rate between the ascending colon module and the transverse colon module is 8-18 mL / min, the flow rate between the transverse colon module and the descending colon module is 35-65 mL / min, the peristaltic compression frequency of the fifth cylindrical reactor and the sixth cylindrical reactor is 3-5 times / minute, the flow rate is 2-4 L / min, the air inflation volume is 350-750 mL, and the compression amplitude is 1.5-3.5 cm; The flow rate between the bionic stomach digestion system and the bionic small intestine digestion system is 15-25 mL / min; the flow rate between the bionic small intestine digestion system and the bionic colon fermentation system is 15-25 mL / min.

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