Application of bacteroides fragilis in preparation of product for preventing or treating chicken testis blood-testis barrier injury

Abstract

The invention relates to the technical field of microorganisms, in particular to application of Bacteroides fragilis in preparation of a product for preventing or treating chicken testis blood testis barrier injury. The invention discloses application of Bacteroides fragilis in preparation of products for preventing or treating chicken testis blood-testis barrier injury for the first time, and experiments prove that the Bacteroides fragilis can improve the weight of chicken testis, the diameter and area of the testis, the number of spermatogonial stem cells in the testis and the expression level of testis blood-testis barrier related protein, and can be used for preparing a product for preventing or treating chicken testis blood-testis barrier injury. The completeness and functions of chicken testis cells can be maintained, and the normal development of the testis can be promoted. The application of the Bacteroides fragilis in the field of reproductive development of chickens is beneficial for promoting the breeding industry to develop towards a more efficient and sustainable direction.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to application of Bacteroides fragilis in preparing a product for preventing or treating blood-testis barrier damage in chicken testicles. Background Art

[0002] With the continuous improvement of people's living standards, the demand for chicken consumption continues to grow, which places higher demands on the production efficiency of the broiler farming industry. As a key guarantee for the reproductive performance of breeding chickens, the normal development of the testicles is directly related to the production and secretion of high-quality sperm and sex hormones. In the physiological structure of the chicken testicle, the blood-testis barrier plays a vital role: on the one hand, it selectively isolates blood from entering the seminiferous tubules, providing an immune-privileged microenvironment for spermatogenesis; on the other hand, as a highly dynamic ultrastructure between supporting cells, it provides structural support and nutritional guarantee for the entire process of spermatogonia developing into mature sperm through meiosis. Studies have shown that once the integrity of the chicken blood-testis barrier is destroyed, harmful substances will invade the lumen of the seminiferous tubules, thereby damaging spermatogenic cells, and ultimately seriously affecting the normal development and reproductive function of the testicles.

[0003] Bacteroides fragilis is a Gram-negative, non-spore-forming anaerobic bacterium. As a common intestinal commensal bacterium, it has multiple physiological functions, mainly participating in nutrient absorption, maintaining intestinal microecological balance, and regulating immune responses. The invention patent with publication number CN114558036A discloses the use of Bacteroides fragilis in improving and treating diarrhea. However, the role of intestinal microorganisms in the reproductive development of chickens is currently unclear, especially the effect of Bacteroides fragilis on the development of chicken testicles. In broiler farming, traditional growth and reproduction methods mainly rely on the use of feed additives and drugs. However, the use of these chemical substances has problems such as drug residues and drug resistance. Therefore, finding a safer and more effective method to promote the growth and reproduction of chickens has become a research hotspot. Summary of the Invention

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide an application of Bacteroides fragilis in the preparation of a product for preventing or treating blood-testis barrier damage in chicken testicles.

[0005] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0006] On the one hand, the invention provides the use of Bacteroides fragilis in preparing a product for preventing damage to the blood-testis barrier of chicken testicles.

[0007] On the other hand, the invention provides the use of Bacteroides fragilis in preparing a product for treating damage to the blood-testis barrier of chicken testicles.

[0008] Furthermore, Bacteroides fragilis can increase the weight, diameter, area, number of spermatogenic stem cells and testicular blood-testis barrier function of chicken testicles.

[0009] The beneficial effects of the present invention are:

[0010] This invention discloses for the first time the use of Bacteroides fragilis in the preparation of a product for preventing or treating damage to the blood-testis barrier in chicken testes. This product helps maintain the integrity and function of chicken testicular cells and promotes normal testicular development. The application of Bacteroides fragilis in the field of chicken reproductive development will help promote the development of the breeding industry towards a more efficient and sustainable direction. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 Schematic diagram of the experimental results of the intestinal microbial bacterial liquid of chickens in the normal development group (CON) and the rapid development group (T2) after gavage of the testicles in Experimental Example 1; wherein, A: testicular weight of chickens in the CON and T2 groups; B: testicular weight of Wenchang chickens after gavage of bacterial liquid; C: correction result of the testicles of Wenchang chickens after gavage of bacterial liquid; D: on-site picture of the testicles after gavage of bacterial liquid; E: H&E stained section of the testicles; F: testicular diameter; G: area of ​​the seminiferous tubules of the testicles; H: area of ​​the testicles; I: number of spermatogenic stem cells in the testis; J: expression results of proteins related to the testicular blood-testis barrier function; K: testicular C-kit immunofluorescence results.

[0012] Figure 2 Schematic diagram of the metagenomic results of the intestinal contents of chickens in the normal testicular development group (CON) and the rapid testicular development group (T2) in Experimental Example 1; A: intestinal microbial flora structure at the microbial genus level; B: intestinal microbial flora structure at the microbial species level; C: differential intestinal microbial flora at the genus level; D: differential intestinal microbial flora at the species level;

[0013] Figure 3 Schematic diagram of the experimental results of the control group and the Bacteroides fragilis gavage group in Experimental Example 3; wherein, A: Wenchang chicken minced body weight; B: Wenchang chicken testis weight; C: testis correction result; D: testis picture taken on site; E: testis diameter; F: testis area; G: testicular spermatogenic stem cell number; H: testicular H&E stained section; I, J: testicular blood-testis barrier function related protein expression results. DETAILED DESCRIPTION

[0014] The specific embodiments of the present invention are described below to facilitate understanding of the present invention by those skilled in the art. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the appended claims, these changes are obvious, and all inventions and creations utilizing the concepts of the present invention are protected.

[0015] Test Example 1

[0016] In this experiment, the testicles and intestinal contents of 100 30-day-old Wenchang chickens were collected and the testicle weight was weighed. According to the testicular weight, the Wenchang chickens were divided into a normal development group (CON) and a testicular rapid development group (T2), with 15 chickens in each group. The intestinal contents of the two groups of chickens were made into bacterial liquid, and the bacterial liquid was gavaged into 1-day-old Wenchang chickens. The experiment lasted for 30 days, with daily gavage at a volume of 500 μL per chicken. The control group was gavaged with the bacterial liquid of the chickens in the normal testicular development group (FMT-CON group), and the experimental group was gavaged with the bacterial liquid of the chickens in the rapid testicular development group (FMT-T2 group).

[0017] The experimental results are as follows Figure 1 As shown, Figure 1 The results in Figure A showed that the testicle weight of chickens in the rapid testicular development group was significantly higher than that in the normal development group, indicating that the normal and rapid testicular development models were successfully established. Figure 1 The results in Figures B and C showed that oral administration of intestinal microbial fluid to the rapidly developing testicles group could increase the testicle weight of Wenchang chickens. Figure 1 Figure D in the middle shows that oral administration of intestinal microbial fluid to the rapid testicular development group can increase the testicular size of Wenchang chickens. Figure 1 The results of EI showed that oral administration of intestinal microbial fluid to the rapid testicular development group could increase the diameter, area and number of spermatogenic stem cells in Wenchang chicken testicles. Figure 1 The results showed that oral administration of intestinal microbial fluid to the rapid testicular development group could improve the blood-testis barrier function of Wenchang chickens. Figure 1 The results of K showed that oral administration of intestinal microbial fluid to the rapid testicular development group could improve the differentiation ability of spermatogenic stem cells in Wenchang chicken testicles.

[0018] Test Example 2

[0019] Metagenomic sequencing technology was used to detect the bacterial community structure of the intestinal contents of the CON and T2 groups of chickens in Experimental Example 1, and to screen for bacterial communities that could potentially promote the testicular blood-testis barrier function.

[0020] The experimental results are as follows Figure 2 As shown, Figure 2 Figures A and B in the middle represent the genus and species structures of the intestinal flora of chickens in the CON group and T2 group, respectively. Figure 2Figures C and D show that the abundance of Bacteroides fragilis in the T2 group was significantly higher than that in the CON group.

[0021] Test Example 3

[0022] In this study, Wenchang chickens were used as the research subjects. The selected Bacteroides fragilis (purchased from Ningbo Testo Biotechnology Co., Ltd., product number ATCC23745) was gavaged into one-day-old Wenchang chickens. The experiment lasted for 30 days, with daily gavage at a volume of 500 μL per chicken. The experiment was designed into two groups: a CON control group and an experimental group gavaged with Bacteroides fragilis. The CON group was gavaged with 500 μL of sterile saline, and the experimental group was gavaged with 500 μL of Bacteroides fragilis bacterial solution (2×10 8 CFU / mL).

[0023] The experimental results are as follows Figure 3 As shown, Figure 3 The results in medium A showed that the weight of Wenchang chicken in the Bacteroides fragilis group increased significantly. Figure 3 The results in Figures B and C showed that the testicle weight of Wenchang chickens in the Bacteroides fragilis group increased significantly. Figure 3 Figure D in the middle shows that oral administration of Bacteroides fragilis can increase the testicle size of Wenchang chickens. Figure 3 The EH results showed that the diameter, area and number of spermatogenic stem cells in the testicles of Wenchang chickens in the Bacteroides fragilis group were significantly higher than those in the CON group. Figure 3 The results in the middle IJ showed that the protein level of Occludin-1 in the Bacteroides fragilis group was higher than that in the CON group, indicating that oral administration of Bacteroides fragilis can improve the blood-testis barrier function of Wenchang chicken testes.

[0024] Studies have shown that intestinal microorganisms may interact with the host's endocrine system, affecting hormone synthesis and secretion. Although it is not yet clear how Bacteroides fragilis specifically affects hormone levels, it may indirectly affect testicular development by regulating the host's endocrine environment. In the current study, Bacteroides fragilis also has antioxidant functions. To a certain extent, it can inhibit certain oxidative reactions in the intestine and reduce the production of free radicals, or enhance the host's antioxidant capacity by regulating the host's antioxidant enzyme system. Antioxidants are important for the protection and development of testicular tissue. Therefore, this potential antioxidant effect of Bacteroides fragilis may also help maintain the integrity and function of testicular cells and promote normal testicular development.

[0025] In summary, this invention discloses for the first time the use of Bacteroides fragilis in the preparation of a product for preventing or treating damage to the blood-testis barrier in chicken testes, helping to maintain the integrity and function of chicken testicular cells and promote normal testicular development. The application of Bacteroides fragilis in the field of chicken reproductive development will help promote the development of the breeding industry in a more efficient and sustainable direction.

[0026] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0027] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. Application of Bacteroides fragilis in the preparation of products for preventing damage to the blood-testis barrier in chicken testicles.

2. Application of Bacteroides fragilis in the preparation of products for treating blood-testis barrier damage in chicken testicles.

3. The use according to claim 1 or 2, characterized in that Bacteroides fragilis can increase the weight, diameter, area, number of spermatogenic stem cells and expression levels of blood-testis barrier-related proteins in chicken testicles.

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