C 12 H 12 Ca3O 15 Application of 3H2O in the photo-excited catalytic synthesis of hydrophobic polysaccharides with high O-acetyl content

Abstract

This invention provides C 12 H 12 Ca3O 15 The application of ·3H2O in the photo-excited catalytic synthesis of hydrophobic polysaccharides with high O-acetyl content also provides the use of C 12 H 12 Ca3O 15 A method for preparing hydrophobic polysaccharides with high O-acetyl content using 3H2O involves adding an alcohol solution and C to aloe vera pulp. 12 H 12 Ca3O 15 ·3H2O was reacted under alternating red and blue light irradiation, filtered, and the filtrate was concentrated to obtain a membrane. This membrane was then freeze-dried to obtain the extract. This invention utilizes C... 12 H 12 Ca3O 15 The application of 3H2O in the preparation of polysaccharides not only increases the polysaccharide content and the content of O-acetyl groups, but more importantly, the resulting polysaccharides have good hydrophobicity, can inhibit inflammation and activate immunity, and have potential value as anti-inflammatory drugs for the treatment of inflammatory diseases. Moreover, the preparation method is simple, green and environmentally friendly, and low in cost.

Description

Technical Field

[0001] This invention belongs to the field of plant extraction technology, specifically involving C 12 H 12 Ca3O 15 Application of 3H2O in the photo-excited catalytic synthesis of hydrophobic polysaccharides with high O-acetyl content. Background Technology

[0002] Polysaccharides, as widely distributed macromolecular polymers in nature, have become a continuous research hotspot in the biomedical field due to their excellent immunomodulatory, antitumor, anti-inflammatory, metabolic regulatory, antibacterial, and antiviral bioactivities, as well as their outstanding stability, biocompatibility, degradability, and low toxicity. To further explore the application potential of polysaccharides, especially to optimize their bioactivity, improve their physicochemical properties, expand their application range, and elucidate their structure-activity relationships, structural modification (such as sulfation, phosphorylation, acetylation, carboxymethylation, and selenization) is an important research strategy.

[0003] Among various modification strategies, acetylation has attracted much attention due to its ability to significantly enhance the biological activity of polysaccharides. The O-acetyl group plays a crucial role in the formation and maintenance of active sites in polysaccharides. Successful acetylation not only effectively enhances the specific biological effects of polysaccharides but also has potential benefits such as improving polysaccharide solubility and acting as a prebiotic to regulate gut microbiota health. Furthermore, the introduction of the acetyl group is one of the core means of regulating the hydrophilic-hydrophobic balance of polysaccharide molecules. This appropriate hydrophobicity is an indispensable physicochemical property for maintaining the basic structure and function of living organisms: it drives and stabilizes cell membrane and protein structures, dominates the selectivity of transmembrane transport, mediates key molecular recognition processes (such as signal transduction and enzyme catalysis), and provides the necessary environmental basis for energy conversion (such as membrane proton gradients and rotating machinery). It can be said that hydrophobic interactions driven by hydrophobicity are the physicochemical cornerstone of cell compartmentalization, the formation of functional conformations of biomolecules, and the efficient energy conversion of life activities. Summary of the Invention

[0004] To address the above problems, the present invention provides C 12 H 12 Ca3O 15 The application of 3H2O in the photo-excited catalytic synthesis of hydrophobic polysaccharides with high O-acetyl content, using C 12 H 12 Ca3O 15 The photo-excitation catalysis of 3H2O can not only significantly increase the content of polysaccharides and O-acetyl groups in the extract, but more importantly, the obtained polysaccharides are hydrophobic, and the method is simple, green and environmentally friendly, and low in cost.

[0005] The C 12 H 12 Ca3O15 ·3H₂O is a calcium-based MOF crystalline material. Its crystal structure belongs to the orthorhombic crystal system, with space group P212121 and cell parameters of _____. a = β = γ = 90°, and the unit cell volume is 1973.65 Å. 3 The chemical structural formula is shown in Formula I:

[0006]

[0007] The C 12 H 12 Ca3O 15 ·3H2O can be prepared by the following method:

[0008] (1) Grind aloe vera leaves into a homogenate in NaCl solution, take the filtrate, and use differential centrifugation to preliminarily separate chloroplasts;

[0009] (2) Pre-cool the chloroplasts described in step (1), and use a combination of precipitation freeze-thaw and high temperature and high pressure to liquefy the chloroplasts to obtain the chloroplast contents;

[0010] (3) Evaporate the chloroplast contents obtained in step (2) at a constant temperature, collect the surface membrane and dissolve it in water, dialyze it to obtain purified chloroplast contents;

[0011] (4) The purified chloroplast contents described in step (3) are freeze-dried, rotary evaporated, and crystallized at low temperature to obtain C. 12 H 12 Ca3O 15 ·3H2O.

[0012] On the other hand, the present invention provides a method for preparing hydrophobic polysaccharides with high O-acetyl content, specifically including the following steps: adding an alcohol solution and C to aloe vera pulp. 12 H 12 Ca3O 15 The mixture was reacted at low temperature under alternating red and blue light irradiation with 3H2O, filtered, and the filtrate was concentrated to obtain a membrane. The membrane was then freeze-dried to obtain the extract.

[0013] Preferably, the aloe vera pulp is obtained by soaking, peeling, and grinding aloe vera leaves.

[0014] Preferably, the grinding is carried out in an ethanol solution with a volume ratio of 70-95%.

[0015] Preferably, the viscosity of the aloe vera pulp is 50-70 mPa·s; more preferably, the viscosity of the aloe vera pulp is 60 mPa·s.

[0016] Preferably, the alcohol solution added to the aloe vera pulp is an ethanol solution.

[0017] Preferably, the concentration of the alcohol solution is 70-95% by volume.

[0018] Preferably, the aloe vera in the aloe vera pulp is related to the C... 12 H 12 Ca3O 15 The mass ratio of aloe vera pulp to C is 600-1200:1; more preferably, the aloe vera pulp and C... 12 H 12 Ca3O 15 The mass ratio of ·3H2O is 800:1.

[0019] Preferably, the duration of alternating red and blue light irradiation is 24-96 hours.

[0020] Preferably, the wavelength of the red light is 620-760nm.

[0021] Preferably, the wavelength of the blue light is 400-500nm.

[0022] Preferably, the light intensity on the liquid surface is >200 lux.

[0023] Preferably, the low-temperature reaction is promoted by mixing at a temperature of 8–10°C.

[0024] Preferably, the filtration is performed by sequentially using filters with a specification of 1000μm and 1μm.

[0025] Preferably, the filtrate is concentrated by constant-temperature evaporation at 60-80°C.

[0026] In another aspect, the present invention provides a hydrophobic polysaccharide with high O-acetyl content prepared by the above preparation method.

[0027] In another aspect, the present invention provides the application of the above-mentioned hydrophobic polysaccharide with high O-acetyl content in the preparation of therapeutic anti-inflammatory drugs.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. This invention will use C 12 H 12 Ca3O 15 The application of 3H2O in the preparation of polysaccharides not only increases the polysaccharide content and the content of O-acetyl groups, but more importantly, the resulting polysaccharides have good hydrophobicity.

[0030] 2. The preparation method of the hydrophobic polysaccharide with high O-acetyl content of the present invention is simple, convenient, green and environmentally friendly, low in cost, does not use organic solvents that are difficult to remove, does not cause degradation of polysaccharides, and the obtained extract is a hydrophobic polysaccharide with high polysaccharide content and high O-acetyl content.

[0031] 3. The hydrophobic polysaccharide with high O-acetyl content of the present invention can inhibit inflammation and activate immunity, and has potential value as an anti-inflammatory drug for treating inflammatory diseases. Attached Figure Description

[0032] Figure 1 C prepared in Example 1 12 H 12 Ca3O 15 • A photograph of 3H2O calcium-based MOF crystal material under an optical microscope;

[0033] Figure 2 C prepared in Example 1 12 H 12 Ca3O 15 • A model diagram of the structure of the 3H2O calcium-based MOF crystal material identified by X-ray diffraction;

[0034] Figure 3 This is a chemical structural diagram of a calcium-based MOF crystal material;

[0035] Figure 4 The figure shows the experimental results of the inhibitory effects of the high aloe polysaccharide and O-acetyl hydrophobic polysaccharide prepared in Example 1 on the inflammatory factors TNF-α, IL-6, and MCP-1; where A represents TNF-α; B represents IL-6; and C represents MCP-1.

[0036] Figure 5 The images show the Raman spectra of the products from Comparative Example 3 and Example 1. Detailed Implementation

[0037] The present invention will be further described in detail below with reference to specific embodiments. The following embodiments are not intended to limit the present invention, but only to illustrate the present invention. Unless otherwise specified, the experimental methods used in the following embodiments are generally performed under conventional conditions. Unless otherwise specified, the materials and reagents used in the following embodiments are commercially available.

[0038] Preparation of C 12 H 12 Ca3O 15 ·3H2O

[0039] (1) Using fresh leaves of Aloe vera as raw material, first remove the head and tail of the fresh leaves, cut off 15-20cm from the front end, remove the tail, rinse with clean water, and soak in purified water for 24 hours.

[0040] (2) Wash the aloe vera leaves soaked in purified water, dry them, remove the epidermis, weigh 50 kg, put them into 150 L of 0.35 mol / L NaCl solution, and grind them into a homogenate using a grinding jar.

[0041] (3) Filter the above homogenate through 6 layers of gauze, take the filtrate, centrifuge at 1000r / min for 5min, discard the precipitate, and take the upper filtrate.

[0042] (4) Centrifuge the above upper filtrate at 3000 r / min for 5 min and collect the precipitate;

[0043] (5) Resuspend the above precipitate with a NaCl solution with a concentration of 0.35 mol / L, centrifuge at 3000 r / min for 20 min, discard the supernatant, and take the chloroplast precipitate;

[0044] (6) Precipitate and freeze chloroplasts: First, pre-cool at 4°C for 30 minutes, then freeze the chloroplasts at a lower temperature (-40°C) for 6 hours.

[0045] (7) Transfer the frozen chloroplast precipitate to room temperature and thaw it until the chloroplasts return to room temperature to accelerate the liquefaction of chloroplasts;

[0046] (8) The chloroplast precipitate that has been restored to room temperature was subjected to high temperature and high pressure treatment at 103.4 kPa and 121℃ for 30 min to further accelerate the liquefaction of chloroplasts.

[0047] (9) Repeat steps (6)-(8) of freezing, thawing at room temperature, and high temperature and high pressure operation three times to obtain chloroplast contents;

[0048] (10) Place the completely liquefied chloroplasts in a constant temperature evaporator and evaporate them at 80°C. A film is formed on the surface. Collect the film every 12 hours for a total of 4 times.

[0049] (11) Combine the membranes collected from the four collections, add 10 times the volume of water, and dialyze continuously for 48 hours through a 10kDa dialysis bag;

[0050] (12) The dialyzed liquid was vacuum freeze-dried for 24 hours, then 2 times the mass of water was added to dissolve it, and the liquid was transferred to a rotary evaporator. The pressure was controlled at 2.5 kPa, the temperature at 45°C, the rotation speed at 160 rpm, and the condenser temperature at 5°C. The liquid was rotary evaporated until the water was completely evaporated. At this time, a slightly yellow, flowable gel appeared.

[0051] (13) Transfer the above gel to a cold storage at 4°C and let it stand for 3 days. Crystals begin to precipitate from the bottom of the gel. Collect the crystals on the 10th day of standing and dry them to constant weight to obtain C. 12 H 12 Ca3O 15 ·3H2O.

[0052] The obtained C was analyzed using a Rigaku SuperNova X-ray single crystal diffractometer.12 H 12 Ca3O 15 X-ray diffraction was performed on 3H₂O, with the following detection conditions: Cu target, Ka rays, wavelength... Voltage 50kV, current 0.8mA The morphology was observed using an optical microscope to obtain the structural morphology and X-ray diffraction pattern of the crystal material. 12 H 12 Ca3O 15 The structural form and formula of 3H2O are as follows: Figure 1 , Figure 2 , Figure 3 As shown in Figure C. 12 H 12 Ca3O 15 • 3H₂O is colorless and transparent, with a rough surface, and has a blocky structure approximately 0.055 mm long, 0.033 mm wide, and 0.021 mm high. Figure 1 ), with the general chemical formula C 12 H 12 Ca3O 15 ·3H2O( Figure 2 and Figure 3 It belongs to the orthorhombic crystal system, with space group P212121 and cell parameters of . a = β = γ = 90°, and the unit cell volume is 1973.65 Å. 3 .

[0053] The crystal material uses L-malic acid (2-hydroxysuccinic acid) as an organic ligand, with all hydroxyl and carboxyl groups in the ligand participating in coordination. Its smallest asymmetric unit consists of three calcium ions, three L-malic acids, and three types of water molecules in different environments (free, bridged, and coordinated). The calcium ions form a three-dimensional network structure with an MBR topology through coordination with L-malic acids, constructing a three-dimensional framework composed of irregular channels.

[0054] Calcium ions coordinate in three ways: 1. The first calcium ion is connected to the second calcium ion through two carboxyl groups and a bridging water group, to the third calcium ion through two carboxyl groups, and to the second and third calcium ions through one carboxyl group; 2. The second calcium ion is connected to the first calcium ion in the aforementioned ways, and is also connected to two other second calcium ions through two carboxyl groups; 3. The third calcium ion is connected to two first calcium ions in the aforementioned two ways, and is also connected to one second calcium ion through one carboxyl group, and has a bridging water group. A free water molecule resides in the cavity formed by one first calcium ion and three third calcium ions, forming a hydrogen bond network with their bridging carboxyl groups.

[0055] L-malic acid exhibits two coordination modes: 1. Two oxygen atoms from the hydroxyl and carboxylic acid groups, respectively, coordinate with a third calcium ion to form a five-membered ring. The remaining oxygen atom of this carboxyl group coordinates with another first or second calcium ion. When the two oxygen atoms of the second carboxyl group simultaneously coordinate with a first or third calcium ion, one of the oxygen atoms also bridges another third or first calcium ion. This malic acid ligand connects a total of 4 calcium ions. 2. An oxygen atom from the carboxylic acid group, together with the oxygen atom of the hydroxyl group, coordinates with a second calcium ion to form a five-membered ring, while also bridging another second calcium ion. The remaining oxygen atom of this carboxyl group coordinates with a first calcium ion. When the two oxygen atoms of the second carboxyl group simultaneously coordinate with a second calcium ion, one of the oxygen atoms also bridges a first calcium ion. This malic acid ligand connects a total of 5 calcium ions.

[0056] Example 1

[0057] (1) Select fresh Aloe vera leaves, cut off the head and tail, that is, cut off 15-20cm from the front end and remove the tail, wash with ultrapure water, and soak in pure water for 24 hours.

[0058] (2) Remove the peel from the aloe vera leaves soaked in pure water and keep the gum. Add 30 kg of 95% (v / v) ethanol to the remaining 240 kg and grind until the viscosity of the aloe vera pulp reaches 60 mPa·s.

[0059] (3) Transfer 270 kg of aloe vera pulp to a 300 L photoreaction tank, add 30 kg of 95% (v / v) ethanol, and add 300 mg of the above-mentioned C. 12 H 12 Ca3O 15 Stir for 48 hours under constant temperature and light conditions of 8-10℃ (light program is red light with wavelength of 620nm for 1 hour, blue light with wavelength of 500nm for 1 hour, and light off for 1 hour, in a cycle). The distance between the light source and the liquid surface is 65cm, and the light intensity on the liquid surface is >200lux.

[0060] (4) After the light exposure ends, the resulting liquid is filtered sequentially through a 1000μm bag filter and a 1μm plate filter, and the filtrate is collected.

[0061] (5) The filtrate was evaporated at 80°C for 12 hours to obtain a membrane, which was then freeze-dried to obtain a powder.

[0062] Example 2

[0063] (1) Select fresh Aloe vera leaves, cut off the head and tail, that is, cut off 15-20cm from the front end and remove the tail, wash with ultrapure water, and soak in pure water for 24 hours.

[0064] (2) Remove the skin from the aloe vera leaves soaked in pure water and keep the gum. Add 30 kg of 95% (v / v) ethanol to the remaining 240 kg and grind until the viscosity of the aloe vera pulp reaches 70 mPa·s.

[0065] (3) Transfer 270 kg of aloe vera pulp to a 300 L photoreaction tank, add 30 kg of 95% (v / v) ethanol, and add 300 mg of C. 12 H 12 Ca3O 15 Stirring for 72 hours under constant temperature and light (lighting program: 1 hour of red light with wavelength of 760 nm, 1 hour of blue light with wavelength of 400 nm, and 1 hour of light off, alternating in a cycle) at 8-10℃ with the light source at a distance of 65 cm from the liquid surface and the light intensity on the liquid surface >200 lux.

[0066] (4) After the light exposure ends, the resulting liquid is filtered sequentially through a 1000μm bag filter and a 1μm plate filter, and the filtrate is collected.

[0067] (5) The filtrate was evaporated at 80°C for 12 hours to obtain a membrane, which was then freeze-dried to obtain a powder.

[0068] Comparative Example 1

[0069] The difference between this example and Example 1 is that 300mg of C is not added before stirring in step (3). 12 H 12 Ca3O 15 ·3H2O. In this example, the filtrate was evaporated at a constant temperature of 80°C for 12 hours to obtain a film-like substance, which was then freeze-dried to obtain a powder.

[0070] Comparative Example 2

[0071] The difference between this example and Example 1 is that the mixture was stirred at a constant temperature of 8-10°C for 48 hours under no light conditions. In this example, no film was obtained after the filtrate was evaporated at a constant temperature of 80°C for 12 hours. The concentrate was then freeze-dried to obtain a powder.

[0072] Comparative Example 3

[0073] The difference between this example and Example 1 is that 300mg of C is not added before stirring in step (3). 12 H 12 Ca3O 15 • 3H₂O was added, and no light was applied during stirring. In this example, no film was obtained after evaporating the filtrate at a constant temperature of 80°C for 12 hours. The concentrate was then freeze-dried to obtain a powder.

[0074] Example of effect

[0075] Detection of polysaccharide content and O-acetyl content

[0076] The powders prepared in Examples 1 and 2, and Comparative Examples 1-3, were subjected to determination of polysaccharide content and O-acetyl content. The determination was carried out according to the phenol-sulfuric acid method in the People's Republic of China Light Industry Standard QB / T2489-2018. The test results are shown in Table 1 below:

[0077] Table 1. Results of polysaccharide and O-acetyl group content detection

[0078]

[0079]

[0080] The results in Table 1 show that, under the same light conditions, the addition of crystals can significantly increase the content of aloe polysaccharides and the content of O-acetyl groups in the powder; with the extension of light exposure time, the content of aloe polysaccharides and the content of O-acetyl groups in the polysaccharides can be increased; light exposure is also a necessary condition for increasing the content of aloe polysaccharides and the content of O-acetyl groups in the polysaccharides, and the formation of film can be used as a basis for judging the increase of polysaccharide content and acetylation content.

[0081] Inflammation suppression effect test

[0082] The effects of the powder prepared in Example 1 on the LPS (Sigma, L2880)-induced inflammation model in RAW264.7 cells (Shanghai Cell Bank, Chinese Academy of Sciences, BFN6080073096) were verified. Four groups were set up: Control group: RAW264.7 cells were treated with DMEM medium for 24 hours; LPS group: mouse RAW264.7 cells were treated with 1 μg / mL LPS for 24 hours; 200 μg / mL Example 1 group: RAW264.7 cells were treated with a solution containing 200 μg / mL of the product from Example 1 and 1 μg / mL LPS for 24 hours; 500 μg / mL Example 1 group: RAW264.7 cells were treated with a solution containing 500 μg / mL of the product from Example 1 and 1 μg / mL LPS for 24 hours. Twenty-four hours later, the cell culture supernatant was collected, and the levels of classic inflammatory factors TNF-α, IL-6, and MCP-1 secreted by cells in the supernatant were detected using an inflammatory cytokine ELISA kit (Elabscience, E-EL-M3063, E-EL-M0044, E-EL-M3001). The results are shown below. Figure 4 As shown, the powder prepared in Example 1 has an inhibitory effect on inflammatory factors, especially IL-6; and the concentration of the extract is positively correlated with the inhibitory effect on inflammatory factors.

[0083] Raman spectroscopy detection

[0084] Weigh 5 mg of the powder obtained in Comparative Example 3 and Example 1, dissolve it thoroughly in water, and aspirate the sample solution using a capillary tube for Raman spectroscopy detection. The test was conducted at room temperature and under atmospheric conditions. The Raman spectra of the samples were acquired using a Renishaw in ViaQontor microconfocal Raman spectrometer. A laser with a wavelength of 532 nm was used for excitation, and the laser power on the sample surface was approximately 26 mW. The single-scan spectral integration time was 10 seconds, and 5 scans were accumulated to improve the signal-to-noise ratio. The scanning range was 0 cm⁻¹. -1 Up to 4000cm -1 The instrument uses a single-crystal silicon wafer (520.7 cm²). -1 Daily calibration is performed, with wavenumber accuracy better than ±1cm. -1 .

[0085] Test results as follows Figure 5 As shown, the sample of Comparative Example 3 can detect the classic Raman spectral peaks of liquid water: 1. OH stretching vibration peak (located at ~3000 cm⁻¹). -1 Up to 3600cm -1 The very broad and asymmetric peak (HOH) is the strongest and most prominent characteristic peak in water Raman spectroscopy, mainly originating from the asymmetric and symmetric OH stretching vibrations of water molecules. However, the width and shape of this peak are not primarily determined by the vibrations of individual water molecules, but rather strongly reflect the strength, distribution, and dynamic changes of intermolecular hydrogen bonds, i.e., the hydrophilicity of the molecules; 3. The bending vibration peak of water HOH (usually reported at approximately 1640 cm⁻¹). -1 The peak of the OH stretching vibration is due to the bending vibration of the HOH bond in water molecules. However, no stretching vibration peak of OH was observed in the test results of Example 1, which means that the polysaccharide obtained in Example 1 has hydrophobic properties and does not form hydrogen bonds with water molecules.

[0086] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. C 12 H 12 Ca3O 15 The application of Ca3O(CO3)2·3H2O in photoexcitation catalytic synthesis of high O-acetyl content hydrophobic polysaccharide is characterized in that, C 12 H 12 Ca3O 15 The method for synthesizing hydrophobic polysaccharides with high O-acetyl content by photoexcitation catalysis using 3H2O includes the following steps: adding an alcohol solution and C to aloe vera pulp. 12 H 12 Ca3O 15 ·3H2O was reacted at low temperature under alternating red and blue light irradiation, filtered, and the filtrate was concentrated to obtain a membrane. This membrane was then freeze-dried to obtain the extract. said aloe vera pulp has a mass ratio of aloe vera to said C 12 H 12 Ca3O 15 ·3H2O of 600-1200:1; The light intensity of the liquid surface is > 200 lux; The duration of the red and blue light alternately irradiating is 24-96 h; The low-temperature reaction adopts a temperature condition of 8-10 ℃; The C 12 H 12 Ca3O 15 ·3H2O is a calcium-based MOF crystal material, the crystal of which belongs to an orthorhombic system, and the space group is P 212121, the cell parameters are a = 7.71360 Å, b = 8.74053 Å, c = 29.2735 Å, a = β = γ = 90°, and the cell volume is 1973.65 A 3; the chemical structural formula is shown as formula I: Formula I.

2. A process for the preparation of a high O-acetyl content hydrophobic polysaccharide, characterized in that, comprising the step of adding an alcoholic solution and C 12 H 12 Ca3O 15 ·3H2O, under the condition of low temperature reaction under the alternate irradiation of red light and blue light, filtration, concentration of the filtrate to obtain a film, freeze-drying to obtain the extract, wherein, said aloe vera pulp has a mass ratio of aloe vera to said C 12 H 12 Ca3O 15 3H2O of 600-1200:1; The light intensity of the liquid surface is > 200 lux; The duration of the red and blue light alternately irradiating is 24-96 h; The low-temperature reaction adopts a temperature condition of 8-10 ℃; The C 12 H 12 Ca3O 15 ·3H2O is a calcium-based MOF crystal material, the crystal of which belongs to an orthorhombic system, and the space group is P 212121, the cell parameters are a = 7.71360 Å, b = 8.74053 Å, c = 29.2735 Å, a = β = γ = 90°, and the cell volume is 1973.65 A 3; the chemical structural formula is shown as formula I: Formula I.

3. The process for preparing a high O-acetyl content hydrophobic polysaccharide according to claim 2, characterized in that, At least one of the following (1)-(3) is included: (1) The wavelength of the red light is 620-760 nm; (2) The wavelength of the blue light is 400-500 nm.

4. The process for preparing a high O-acetyl content hydrophobic polysaccharide according to claim 2, characterized in that, At least one of the following (1)-(2) is included: (1) The filtering is sequentially adopting filters with specifications of 1000 μm and 1 μm; (2) The filtrate is concentrated by constant-temperature evaporation at 60-80 ℃.

5. The process for preparing a high O-acetyl content hydrophobic polysaccharide according to claim 2, characterized in that, The viscosity of the aloe pulp is 50-70 mPa・s.

6. The process for preparing a high O-acetyl content hydrophobic polysaccharide according to claim 2, characterized in that, At least one of the following (1)-(3) is included: (1) The aloe pulp is obtained by soaking, peeling and grinding aloe leaves; (2) The alcohol solution added in the aloe pulp is an ethanol solution; (3) The concentration of the alcohol solution is 70-95% by volume.

7. The process for preparing a high O-acetyl content hydrophobic polysaccharide according to claim 5, characterized in that, At least one of the following (1)-(2) is included: (1) the aloe pulp and the C 12 H 12 Ca3O 15 ·3H2O is 800: 1; (2) The viscosity of the aloe pulp is 60 mPa・s.

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