A cultivation method of high-yield and high-quality phellinus

Abstract

The present application belongs to the technical field of fungus cultivation, and particularly relates to a cultivation method of high-yield and high-quality Phellinus baumii. The cultivation method comprises the following steps: (1) inoculation: preparing a culture medium, filling the culture medium into a plastic bag, sterilizing and cooling, and inoculating with Phellinus baumii spores; (2) fungus management: avoiding light cultivation of the inoculated plastic bag; when the fungus management is performed for 30-35 days, the fungus bag is longitudinally squeezed and shortened, and the cultivation is continued to enter the fruiting management; (3) fruiting management: transferring the matured Phellinus baumii fungus bag into a fruiting greenhouse, drawing a rectangular fruiting opening in the middle of the fungus bag, and performing the Phellinus baumii fruiting management; (4) harvesting: obtaining the Phellinus baumii fruiting body after 100-120 days after the opening is drawn. The present application provides a cultivation method of high-yield and high-quality Phellinus baumii, which significantly improves the biological conversion rate and effective component content of Phellinus baumii by optimizing the culture medium components and ratio, and solves the technical problems of low yield and low effective component content in traditional cultivation.

Description

Technical Field

[0001] This invention belongs to the field of fungal cultivation technology, specifically relating to a cultivation method for high-yield and high-quality Phellinus linteus. Background Technology

[0002] Sanghuang is a precious medicinal fungus. Its medicinal record comes from "Sang'er" in the Chinese herbal classic "Shennong Bencao Jing". Sanghuang contains a variety of active ingredients such as polysaccharides, polyphenols, terpenes, and steroids. It has a variety of biological activities such as anti-tumor, antioxidant, anti-inflammatory, and immunomodulatory effects, and is widely used in traditional Chinese medicine.

[0003] The market demand for Phellinus linteus remains high. Due to the scarcity of its wild resources, artificial cultivation has become the main way to meet market supply. However, because the annual yield of wild Phellinus linteus is less than 5 tons, and large, high-quality fruiting bodies require more than 10 years to grow, coupled with the surge in market demand for high-grade, large-sized Phellinus linteus with high content of effective components (such as triterpenoids), its price remains high.

[0004] Chinese Patent Publication No. CN112931059A discloses a mulberry *Phellinus linteus* strain and its cultivation method. The specific strain is *Phellinus linteus* QJF-2, with accession number CGMCC No. 19653. The *Phellinus linteus* QJF-2 strain provided by this invention has high medicinal value, is authentic mulberry *Phellinus linteus*, and its efficacy is significantly higher than that of common poplar *Phellinus linteus*.

[0005] Chinese Patent No. CN104381009B discloses an artificial bag cultivation process for Phellinus linteus, which includes the following steps: (1) After purification, the strain isolated from the fruiting body of wild Phellinus linteus is inoculated into the mother culture medium to obtain Phellinus linteus mother culture, and the Phellinus linteus mother culture is inoculated into the original culture medium to obtain Phellinus linteus original culture; the Phellinus linteus original culture is inoculated into the cultivation culture medium to obtain Phellinus linteus spawn; (2) Phellinus linteus spawn is inoculated into the spawn stick for spawn cultivation; (3) spawn management.

[0006] Currently, although Japan and South Korea have achieved large-scale cultivation of Phellinus linteus, they mainly rely on poplar log cultivation technology, which has limitations such as high substrate costs and long growth cycles (4-5 years), making it difficult to respond quickly to market changes. High-grade Phellinus linteus with high content of effective components produced using substrate-based cultivation methods has low yields, making it difficult to meet medicinal standards and the demands of the high-end market. Summary of the Invention

[0007] The purpose of this invention is to provide a cultivation method for high-yield and high-quality Phellinus linteus.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A cultivation method for high-yield and high-quality Phellinus linteus includes the following steps:

[0010] (1) Inoculation: Prepare the culture medium, which includes the following components: sugarcane bagasse, rice bran, rapeseed cake powder, plant extract residue, gypsum, and the remainder is mulberry sawdust; put the culture medium into a plastic bag, sterilize and cool it, and then inoculate it with Phellinus linteus;

[0011] (2) Mycelium growth management: After inoculation, the plastic bags are kept in the dark for cultivation; after 30-35 days of mycelium growth management, the bags are compressed longitudinally to shorten them, and cultivation continues for 50-60 days. When the mycelium color changes from yellow to brown, the fruiting management begins.

[0012] (3) Fruiting management: Transfer the mature Sanghuang mushroom bags to the fruiting greenhouse, make a rectangular fruiting opening in the middle of the mushroom bag, and carry out Sanghuang fruiting management;

[0013] (4) Harvesting: 100-120 days after the incision is made, the fruiting bodies of Phellinus linteus are obtained.

[0014] Preferably, the culture medium formulation comprises the following components by mass percentage: 15-20% fermented sugarcane bagasse, 5-10% rice bran, 3-5% rapeseed cake powder, 10-15% plant extract residue, 1-1.5% gypsum, and the remainder being mulberry sawdust.

[0015] Preferably, the method for preparing fermented sugarcane bagasse includes the following steps:

[0016] S1: Dry the sugarcane bagasse, crush it, and sieve it to obtain sugarcane bagasse powder;

[0017] S2: Add water to sugarcane bagasse powder to obtain a mixture, add cellulase to the mixture for enzymatic hydrolysis; after enzymatic hydrolysis, inactivate the enzyme to obtain the hydrolyzed material;

[0018] S3: Add compound bacteria to the enzymatic hydrolysate and ferment it. After fermentation, sterilize it to obtain fermented material. Concentrate the fermented material under reduced pressure and dry it to obtain fermented sugarcane bagasse.

[0019] Preferably, the enzymatic hydrolysis conditions are: 40-50℃, pH adjusted to 5.0-6.0, and enzymatic hydrolysis for 8-10 hours.

[0020] Preferably, the compound bacteria include Bacillus subtilis and Lactobacillus remus var. mansoni.

[0021] Preferably, the viable count of the compound bacteria in the enzymatic hydrolysate is 10. 6 -10 7 CFU / mL, the viable ratio of Bacillus subtilis and Lactobacillus reinhardtii in the compound bacteria was 1:(1.2-1.4).

[0022] Preferably, the plant extract residue includes at least one of the following: Salvia miltiorrhiza extract residue, Ginkgo biloba leaf extract residue, and Drynaria fortunei extract residue.

[0023] Preferably, the plant extract residue includes Salvia miltiorrhiza extract residue, Ginkgo biloba extract residue, and Drynaria fortunei extract residue in a mass ratio of 1:(1.3-1.5):(0.4-0.7).

[0024] The preferred conditions for cultivation in the dark are: temperature controlled at 25-28℃, humidity at 50-55%, and ventilation for 30-50 minutes every morning.

[0025] Preferably, the temperature for mushroom cultivation management of Sanghuang is 25-28℃, and the humidity is controlled at 80-90%.

[0026] The extract residues of Salvia miltiorrhiza, Ginkgo biloba, and Drynaria fortunei in this invention are all extract residues of traditional Chinese medicine. The preparation method is as follows: after pulverizing the raw materials of traditional Chinese medicine, add 10-15 times the amount of water, use a 30%-50% ethanol aqueous solution, reflux at 60-80℃ for 1-2 hours, and repeat the extraction 2-3 times to obtain the residue.

[0027] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0028] 1. This invention provides a high-yield, high-quality cultivation method for Phellinus linteus. By optimizing the composition and ratio of the culture medium, the bioconversion rate and content of effective components (polysaccharides and triterpenes) of Phellinus linteus are significantly improved, solving the technical problems of low yield and low content of effective components in traditional cultivation. The cultivation method of this invention enables a stable supply of high-quality Phellinus linteus, which can meet the growing market demand for pharmaceuticals, health products, and functional foods.

[0029] 2. This invention utilizes sugarcane bagasse from sugar factories and residues from traditional Chinese medicine extracts as raw materials for the culture medium. This not only effectively utilizes agricultural and pharmaceutical waste and reduces production costs, but also significantly reduces environmental pollution from waste, aligning with the concept of "green manufacturing." This invention is not only innovative and practical at the technical level, but also has significant implications for promoting sustainable, green, and high-quality development.

[0030] 3. The cultivation method of this invention significantly increases the yield of premium and first-grade Sanghuang mushrooms, possessing high economic value and contributing to the transformation of the Sanghuang industry from traditional extensive to modern high-efficiency production. High-yield, high-quality Sanghuang products can better meet the demand for high-quality raw materials in multiple fields such as medicine, health care, and food, further enhancing market competitiveness. Simultaneously, the subsequent promotion and application of this technology will drive local economic development, increase farmers' income, and demonstrate significant social benefits and promising industrial development prospects. Attached Figure Description

[0031] Figure 1 A schematic diagram illustrating the inoculation process for the cultivation of Phellinus linteus.

[0032] Figure 2 This is a schematic diagram of the cultivation and mycelium growth of Phellinus linteus.

[0033] Figure 3 A schematic diagram illustrating the cultivation and fruiting of Sanghuang mushroom.

[0034] Figure 4 This is a schematic diagram of the finished product of Sanghuang cultivation. Detailed Implementation

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] All raw materials used in the following embodiments of the present invention are commercially available products:

[0037] Sugarcane bagasse comes from sugar mills.

[0038] Cellulase, Shanghai Yuanye Biotechnology Co., Ltd., Product No. S10041.

[0039] Lactobacillus reinhardtii, product number HZB358615, Zhengzhou Fangjue Biotechnology Co., Ltd.

[0040] Bacillus subtilis, product number HZB134852, Zhengzhou Fangjue Biotechnology Co., Ltd.

[0041] Bacillus amyloliquefaciens, product number HZB122164, Zhengzhou Fangjue Biotechnology Co., Ltd.

[0042] Aspergillus oryzae, product number HZB120226, Wuhan Gray Algae Biotechnology Co., Ltd.

[0043] The extract residues from Salvia miltiorrhiza, Ginkgo biloba, and Drynaria fortunei were all obtained from Sichuan Xiaoye Herbal Biotechnology Co., Ltd. Specifically, the herbal raw materials were pulverized, 10 times the amount of water was added, and a 40% (v / v) ethanol aqueous solution was used. The mixture was refluxed at 70°C for 1.5 hours, and the extraction was repeated three times to obtain the residues.

[0044] The *Inonotus sanghuang* strain used in this invention was obtained from the China General Microbiological Culture Collection Center (CGMCC), with the number CGMCC NO: 8854. The *Inonotus sanghuang* strain was inoculated onto a primary culture medium to obtain the primary sanghuang culture; the primary sanghuang culture was then inoculated onto a cultivation culture medium to obtain the cultivated sanghuang culture. Both the mother culture medium and the primary culture medium were composed of a mixture of sawdust, wheat bran, and corn cobs in equal proportions.

[0045] Example 1

[0046] This embodiment provides a cultivation method for high-yield and high-quality Sanghuang, including the following steps:

[0047] (1) Inoculation: Prepare the culture medium, which includes the following components by mass percentage: 16% fermented sugarcane bagasse, 8% rice bran, 4% rapeseed cake powder, 12% plant extract residue, 1% gypsum, and the remainder is mulberry sawdust. The plant extract residue includes Salvia miltiorrhiza extract residue, Ginkgo biloba leaf extract residue and Drynaria fortunei extract residue in a mass ratio of 1:1.4:0.6. The inoculum bag is a 14cm×27cm×0.05mm polyethylene plastic bag. The culture medium is put into the plastic bag, sterilized and cooled to 27°C. In a sterile environment, Phellinus linteus cultivar is inoculated into the culture medium at an inoculation amount of 8% of the culture medium mass.

[0048] (2) Mycelium growth management: After inoculation, the plastic bags are kept in the dark and the temperature is controlled at 26℃ and the humidity at 50%. Ventilate for 40 minutes every day starting at 9:00 am. Check the bags by turning them over once every 15 days and remove the contaminated bags from the cultivation room. After 30 days of mycelium growth management, the bags are shortened by 2 cm by longitudinal compression and cultured for another 55 days. When the mycelium color changes from yellow to brown, the fruiting management begins.

[0049] (3) Fruiting management: Transfer the mature Sanghuang mushroom bags to the fruiting greenhouse. Make a rectangular fruiting opening with a length of 10cm and a width of 4cm in the middle of the mushroom bag. The distance between mushroom bags is 15cm. The fruiting temperature of Sanghuang is 28℃ and the humidity is controlled at 85%.

[0050] (4) Harvesting: The fruiting bodies of Phellinus linteus are obtained 120 days after the incision is made.

[0051] The method for preparing fermented sugarcane bagasse includes the following steps:

[0052] S1: Dry the sugarcane bagasse, pulverize it, and pass it through a 20-mesh sieve to obtain sugarcane bagasse powder;

[0053] S2: Add water to bagasse powder to make a mixture with a mass concentration of 35% bagasse powder. Add cellulase to the mixture and hydrolyze for 9 hours at 45°C and pH adjusted to 6.0. The amount of cellulase used is 110 U / mL of the mixture. After hydrolysis, inactivate the enzyme to obtain the hydrolyzed material.

[0054] S3: Add a compound microbial culture to the enzymatic hydrolysate. The compound microbial culture includes Bacillus subtilis and Lactobacillus reinhardtii, and the viable count of the compound microbial culture in the enzymatic hydrolysate is 10. 6 The ratio of live bacteria of Bacillus subtilis and Lactobacillus reinhardtii in the compound culture was 1:1.3 (CFU / mL). Aerobic fermentation was carried out for 27 days with an air flow rate of 0.5 cubic meters per minute. After fermentation, the mixture was sterilized to obtain fermented material. The fermented material was then concentrated under reduced pressure and dried to obtain fermented sugarcane bagasse.

[0055] Example 2

[0056] This embodiment provides a cultivation method for high-yield and high-quality Sanghuang, including the following steps:

[0057] (1) Inoculation: Prepare the culture medium, which includes the following components by mass percentage: 15% fermented sugarcane bagasse, 10% rice bran, 3% rapeseed cake powder, 15% plant extract residue, 1% gypsum, and the remainder is mulberry sawdust. The plant extract residue includes Salvia miltiorrhiza extract residue, Ginkgo biloba extract residue and Drynaria fortunei extract residue in a mass ratio of 1:1.3:0.7. The inoculum bag is a 14cm×27cm×0.05mm polyethylene plastic bag. The culture medium is put into the plastic bag, sterilized and cooled to 25°C, and then inoculated with Phellinus linteus.

[0058] (2) Mycelium growth management: After inoculation, the plastic bags are kept in the dark and the temperature is controlled at 26℃ and the humidity at 50%. Ventilate for 40 minutes every day starting at 9:00 am. Check the bags by turning them over once every 15 days and remove the contaminated bags from the cultivation room. After 30 days of mycelium growth management, the bags are shortened by 2 cm by longitudinal compression and cultured for another 55 days. When the mycelium color changes from yellow to brown, the fruiting management begins.

[0059] (3) Fruiting management: Transfer the mature Sanghuang mushroom bags to the fruiting greenhouse. Make a rectangular fruiting opening with a length of 10cm and a width of 4cm in the middle of the mushroom bag. The distance between mushroom bags is 15cm. The fruiting temperature of Sanghuang is 28℃ and the humidity is controlled at 85%.

[0060] (4) Harvesting: The fruiting bodies of Phellinus linteus are obtained 120 days after the incision is made.

[0061] The method for preparing fermented sugarcane bagasse includes the following steps:

[0062] S1: Dry the sugarcane bagasse, pulverize it, and pass it through a 20-mesh sieve to obtain sugarcane bagasse powder;

[0063] S2: Add water to bagasse powder to make a mixture with a bagasse powder mass concentration of 35%. Add cellulase to the mixture and hydrolyze for 8 hours at 40℃ and pH adjusted to 6.0. The amount of cellulase used is 100 U / mL of the mixture. After the hydrolysis is completed, inactivate the enzyme to obtain the hydrolysate.

[0064] S3: Add a compound microbial culture to the enzymatic hydrolysate. The compound microbial culture includes Bacillus subtilis and Lactobacillus reinhardtii, and the viable count of the compound microbial culture in the enzymatic hydrolysate is 10. 6 The ratio of live bacteria of Bacillus subtilis and Lactobacillus reinhardtii in the compound bacteria was 1:1.4 (CFU / mL). Aerobic fermentation was carried out for 30 days with an air flow rate of 0.5 cubic meters per minute. After fermentation, the mixture was sterilized to obtain fermented material. The fermented material was then concentrated under reduced pressure and dried to obtain fermented sugarcane bagasse.

[0065] Comparative Example 1

[0066] The difference between this comparative example and Example 1 is that the culture medium used in the artificial bag cultivation process of Sanghuang in Chinese Patent No. CN104381009B has the following weight percentages of each raw material component: wheat bran 10%, corn flour 10%, gypsum powder 1%, and the remainder is mulberry branch sawdust.

[0067] Comparative Example 2

[0068] The difference between this comparative example and Example 1 is that no plant extract residues were added to the culture medium.

[0069] The culture medium formula includes the following components by mass percentage: 16% fermented sugarcane bagasse, 8% rice bran, 4% rapeseed cake powder, 1% gypsum, and the remainder is mulberry sawdust.

[0070] Comparative Example 3

[0071] The difference between this comparative example and Example 1 is that fermented sugarcane bagasse is not added to the culture medium.

[0072] The culture medium formula includes the following components by weight percentage: 8% rice bran, 4% rapeseed cake powder, 12% plant extract residue, 1% gypsum, and the remainder is mulberry sawdust.

[0073] Comparative Example 4

[0074] The difference between this comparative example and Example 1 is that the sugarcane bagasse is not fermented. The sugarcane bagasse is processed by drying, crushing, and passing through a 20-mesh sieve.

[0075] The culture medium formula includes the following components by weight percentage: 16% sugarcane bagasse, 8% rice bran, 4% rapeseed cake powder, 12% plant extract residue, 1% gypsum, and the remainder is mulberry sawdust.

[0076] Comparative Example 5

[0077] The difference between this comparative example and Example 1 is that the compound bacteria were replaced with Bacillus subtilis.

[0078] Comparative Example 6

[0079] The difference between this comparative example and Example 1 is that the compound bacteria include Bacillus subtilis and Bacillus amyloliquefaciens. The viable count of the compound bacteria in the enzymatic hydrolysate is 10. 6 The CFU / mL ratio of Bacillus subtilis to Bacillus amyloliquefaciens in the enzymatic hydrolysate was 1:1.3.

[0080] Comparative Example 7

[0081] The difference between this comparative example and Example 1 is that the compound bacteria include Bacillus subtilis and Aspergillus oryzae. The viable count of the compound bacteria in the enzymatic hydrolysate is 10.6 The CFU / mL ratio of Bacillus subtilis to Aspergillus oryzae in the enzymatic hydrolysate was 1:1.3.

[0082] Comparative Example 8

[0083] The difference between this comparative example and Example 1 is that the ratio of live bacteria of Bacillus subtilis and Lactobacillus reinhardtii in the compound bacteria is 1:1.

[0084] Comparative Example 9

[0085] The difference between this comparative example and Example 1 is that the ratio of viable Bacillus subtilis and Lactobacillus reinhardtii in the compound bacteria is 1:1.5.

[0086] Comparative Example 10

[0087] The difference between this comparative example and Example 1 is that the plant extract residue is the residue of Salvia miltiorrhiza extract.

[0088] Comparative Example 11

[0089] The difference between this comparative example and Example 1 is that the plant extract residue is apricot leaf extract residue.

[0090] Comparative Example 12

[0091] The difference between this comparative example and Example 1 is that the plant extract residue is the extract residue of Drynaria fortunei.

[0092] Comparative Example 13

[0093] The difference between this comparative example and Example 1 is that the plant extract residues include Salvia miltiorrhiza extract residues, Ginkgo biloba leaf extract residues, and Drynaria fortunei extract residues in a mass ratio of 1.4:0.6:1.

[0094] Comparative Example 14

[0095] The difference between this comparative example and Example 1 is that the plant extract residue includes Salvia miltiorrhiza extract residue, Ginkgo biloba extract residue and Drynaria fortunei extract residue in a mass ratio of 0.6:1:1.4.

[0096] Performance testing

[0097] (1) The cultivation methods of Examples 1-2 and Comparative Examples 1-14 were followed. Each group consisted of 18 bags, and two flushes of fruiting bodies were harvested. Yield, biological efficiency, crude polysaccharide content, and triterpenoid content were statistically analyzed. The determination methods were based on "The Influence of Different Cultivation Substrate Formulas on the Growth Characteristics of Wild Phellinus linteus Fruiting Bodies" (Du Chengzhi et al.). The results are shown in Table 1.

[0098] Table 1 Statistical results of growth characteristics of Phellinus linteus

[0099]

[0100]

[0101] As shown in Table 1, the culture media of Examples 1-2 of this invention have high bioconversion rates, high bag yields, and higher contents of polysaccharides and triterpenes than the culture media of Comparative Example 1 using the prior art. The results indicate that fermenting sugarcane bagasse and plant extracts can increase the content of polysaccharides and triterpenes in Phellinus linteus; the absence of any one of these components leads to a decrease in the yield of Phellinus linteus and a decrease in the content of polysaccharides and triterpenes.

[0102] (2) Referring to the grading standards of substitute-grade Sanghuang in Table 2 of NY / T4075-2022 "Sanghuang Grading Specifications", the cultivation methods of Examples 1-2 and Comparative Examples 1-14 were statistically analyzed, and the percentage of the weight of premium and first-grade Sanghuang in the total weight of Sanghuang fruiting bodies from the two harvests was recorded. The results are shown in Table 2.

[0103] Table 2. Specific gravity (wt%) of different grades of Sanghuang

[0104]

[0105]

[0106] The *Sanghuang* strain with CGMCC NO: 8854 is a widely cultivated strain in the market, but it suffers from low yields of high-quality *Sanghuang*. Table 2 shows that the *Sanghuang* strains from Examples 1-2 exhibited high yields of premium and first-grade strains, significantly superior to those cultivated using commonly used culture media in existing technologies. The results indicate that fermented sugarcane bagasse affects the grade of *Sanghuang*, and the type and ratio of plant extracts also influence its grade. These two components work synergistically to increase the yield of high-quality *Sanghuang*.

[0107] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A cultivation method for high-yield and high-quality Phellinus linteus, characterized in that, Includes the following steps: (1) Inoculation: Prepare the culture medium, which includes the following components: sugarcane bagasse, rice bran, rapeseed cake powder, plant extract residue, gypsum, and the remainder is mulberry sawdust; put the culture medium into a plastic bag, sterilize and cool it, and then inoculate it with Phellinus linteus. (2) Mycelium growth management: After inoculation, the plastic bags are kept in the dark for cultivation; after 30-35 days of mycelium growth management, the bags are shortened by longitudinal compression and cultivation is continued for 50-60 days. When the mycelium color changes from yellow to brown, the fruiting management begins. (3) Fruiting management: Transfer the mature Sanghuang mushroom bags to the fruiting greenhouse, make a rectangular fruiting opening in the middle of the mushroom bag, and carry out Sanghuang fruiting management; (4) Harvesting: The fruiting bodies of Phellinus linteus are obtained 100-120 days after the incision is made; The culture medium formula includes the following components by mass percentage: 15-20% fermented sugarcane bagasse, 5-10% rice bran, 3-5% rapeseed cake powder, 10-15% plant extract residue, 1-1.5% gypsum, and the balance being mulberry wood chips; the plant extract residue includes Salvia miltiorrhiza extract residue, Ginkgo biloba leaf extract residue, and Drynaria fortunei extract residue in a mass ratio of 1:(1.3-1.5):(0.4-0.7); The method for preparing fermented sugarcane bagasse includes the following steps: S1: Dry the sugarcane bagasse, crush it, and sieve it to obtain sugarcane bagasse powder; S2: Add water to sugarcane bagasse powder to obtain a mixture, add cellulase to the mixture for enzymatic hydrolysis; after enzymatic hydrolysis, inactivate the enzyme to obtain the hydrolyzed material; S3: Add compound bacteria to the enzymatic hydrolysate for fermentation. After fermentation, sterilize to obtain fermented material. Concentrate the fermented material under reduced pressure and dry it to obtain fermented sugarcane bagasse. The compound bacteria include Bacillus subtilis and Lactobacillus reinhardtii; the viable count of the compound bacteria in the enzymatic hydrolysate is 10. 6 -10 7 CFU / mL, the viable ratio of Bacillus subtilis and Lactobacillus reinhardtii in the compound bacteria was 1:(1.2-1.4).

2. The cultivation method for high-yield and high-quality Sanghuang according to claim 1, characterized in that, The conditions for enzymatic hydrolysis are: 40-50℃, pH adjusted to 5.0-6.0, and enzymatic hydrolysis for 8-10 hours.

3. The cultivation method for high-yield and high-quality Sanghuang according to claim 1, characterized in that, The conditions for cultivation in the dark are: temperature controlled at 25-28℃, humidity at 50-55%, and ventilation for 30-50 minutes every morning.

4. The cultivation method for high-yield and high-quality Sanghuang according to claim 1, characterized in that, The optimal temperature for mushroom cultivation of Sanghuang is 25-28℃, and the humidity should be controlled at 80-90%.

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