Pleurotus geesteranus compost and cultivation production method

Abstract

The invention provides a pleurotus geesteranus compost and a cultivation production method, and belongs to the technical field of edible mushroom cultivation. The pleurotus geesteranus culture material comprises the following raw materials in parts by weight: 40-60 parts of litchi sawdust, 20-40 parts of cottonseed hull, 10-20 parts of wheat bran, 1-2 parts of gypsum powder, 1-2 parts of quick lime and 1-2 parts of sucrose. The cultivation production method comprises the steps of culture material preparation, inoculation, spawn running management, fruiting management, harvesting and nutrient solution supplement to cultivate downtide mushrooms. The litchi wood waste is used as the raw material of the pleurotus geesteranus culture material, high-valued cyclic utilization of agricultural resources is achieved, growth of pleurotus geesteranus hyphae can be promoted, the yield and quality of sporocarp are improved, and the method is easy and convenient to operate and suitable for large-scale popularization and has important significance in promoting green and sustainable development of the pleurotus geesteranus industry.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a Pleurotus geesteranus culture medium and a cultivation production method. Background Art

[0002] As a nutritious and delicious edible mushroom, Pleurotus geesterani Singer is highly favored by the market and consumers, and its cultivation scale has continued to expand in recent years. The formulation and quality of the culture medium are key factors influencing growth rate, yield, and quality during Pleurotus geesteranus cultivation. Traditional Pleurotus geesteranus culture mediums primarily use sawdust, cottonseed hulls, and corncobs. However, these materials suffer from unstable raw material sources and unbalanced nutritional profiles. Some regions also face a shortage of sawdust resources and significant price fluctuations, hindering the sustainable development of the Pleurotus geesteranus industry.

[0003] At the same time, my country has a vast lychee planting area, and the annual pruning produces a huge amount of lychee wood branches and discarded trunks. Most of these lychee wood resources are burned or discarded at random, which not only wastes resources but also causes environmental pollution. Lychee wood is rich in natural organic components such as cellulose, hemicellulose, and lignin. In theory, it can be used as a high-quality raw material for edible fungus culture. However, there is currently no mature technical solution for the scientific application of lychee wood in the large-scale cultivation of Pleurotus geesteranus. Therefore, the development of Pleurotus geesteranus culture media and supporting cultivation methods using lychee wood as the core raw material can not only solve the bottleneck problem of traditional culture media raw materials, but also realize the high-value utilization of lychee wood resources, which is of great significance to promoting the green development of the Pleurotus geesteranus industry. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a Pleurotus geesteranus culture medium and a cultivation production method, using litchi wood as the raw material for Pleurotus geesteranus culture medium, promoting the growth of Pleurotus geesteranus, increasing the yield of Pleurotus geesteranus, and improving the comprehensive utilization rate of litchi wood.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] A Pleurotus geesteranus culture medium comprises the following raw materials in parts by weight: 40-60 parts of litchi sawdust, 20-40 parts of cottonseed hulls, 10-20 parts of wheat bran, 1-2 parts of gypsum powder, 1-2 parts of quicklime and 1-2 parts of sucrose.

[0007] Preferably, the lychee wood is fresh lychee wood that is dried in the shade for 5 to 6 months, rotten branches are removed, and then crushed into 0.6 to 1 cm.

[0008] Preferably, the water content of the culture medium is 62% to 65%.

[0009] The present invention also provides a method for preparing the above-mentioned culture medium, comprising the following steps: mixing the raw materials, adding water and stirring evenly, putting them into fungus bags, sterilizing them at 110-120° C. for 1-2 hours, and cooling them to room temperature.

[0010] The present invention also provides application of the culture medium in the cultivation of Pleurotus geesteranus.

[0011] The present invention also provides a cultivation and production method of Pleurotus geesteranus, comprising the following steps: inoculating the Pleurotus geesteranus strain into the above-mentioned culture medium, performing spawning and fruiting management, harvesting the first-batch mushrooms after they mature, replenishing nutrient solution, and performing spawning and fruiting management of the Pleurotus geesteranus again.

[0012] Preferably, during the spawn management stage, the temperature is 20-25° C., the air humidity is 75%-85%, the lighting conditions are 300-500 lx scattered light, and ventilation is performed in the morning and evening.

[0013] Preferably, during the mushroom production management stage, the temperature is 18-24° C. and the air humidity is 80%-90%.

[0014] Preferably, the formula of the nutrient solution is: glucose 80-120 g / L, ammonium sulfate 8-12 g / L, potassium dihydrogen phosphate 4-6 g / L, magnesium sulfate 1.5-3 g / L, calcium chloride 0.5-1.5 g / L and vitamin B1 0.05-0.2 g / L.

[0015] More preferably, the nutrient solution is supplemented by soaking the fungus bag in the nutrient solution for 10 to 20 minutes to allow the nutrient solution to fully penetrate into the culture medium.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] This invention transforms lychee branches and trunks, traditionally burned or discarded, into high-quality culture medium for Pleurotus geesteranus cultivation. This not only addresses the waste of lychee wood resources and environmental pollution, but also reduces Pleurotus geesteranus cultivation's reliance on traditional raw materials such as sawdust, achieving high-value recycling of agricultural waste. The culture medium of this invention can increase the growth rate of Pleurotus geesteranus mycelium, shorten the spawning cycle, and improve production efficiency. It can also increase the yield and quality of Pleurotus geesteranus fruiting bodies, thereby enhancing economic benefits. DETAILED DESCRIPTION

[0018] The present invention provides a culture medium for Pleurotus geesteranus, comprising the following raw materials, by weight: 40-60 parts of lychee sawdust, 20-40 parts of cottonseed hulls, 10-20 parts of wheat bran, 1-2 parts of gypsum powder, 1-2 parts of quicklime, and 1-2 parts of sucrose; preferably, 45-55 parts of lychee sawdust, 25-35 parts of cottonseed hulls, 12-18 parts of wheat bran, 1-1.5 parts of gypsum powder, 1-1.5 parts of quicklime, and 1-1.5 parts of sucrose; more preferably, 50 parts of lychee sawdust, 30 parts of cottonseed hulls, 17 parts of wheat bran, 1 part of gypsum powder, 1 part of quicklime, and 1 part of sucrose. In this ratio, the lychee sawdust provides a rich carbon source such as cellulose and lignin, the cottonseed hulls ensure the air permeability and water retention of the culture medium, the wheat bran supplements the nitrogen source and vitamins, the gypsum powder and quicklime adjust the pH of the culture medium to a suitable range for Pleurotus geesteranus, and the sucrose quickly replenishes energy, thereby achieving a balanced nutritional balance to meet the needs of Pleurotus geesteranus at different growth stages.

[0019] In the present invention, fresh lychee wood is preferably shade-dried for 5-6 months, rotten branches removed, and then crushed to 0.6-1 cm, more preferably to 0.8 cm. Fresh lychee wood contains components that are detrimental to the growth of P. geesteranus. The shade-drying process naturally degrades volatile harmful substances (such as some phenolic substances) in the lychee wood, reducing its inhibitory effect on P. geesteranus mycelium. Removing rotten branches can reduce the risk of contamination by other bacteria. The 0.6-1 cm crushing particle size balances the air permeability and water retention of the culture medium, ensuring the oxygen required for mycelium respiration while preventing rapid water loss, which is conducive to mycelium colonization and spread.

[0020] In the present invention, the moisture content of the compost is preferably 62% to 65%, more preferably 63% to 64%. This appropriate moisture content keeps the compost moist but not waterlogged, meeting the moisture requirements of P. geesteranus mycelium while preventing problems such as hypoxia and bacterial growth (e.g., mold contamination) caused by excessive moisture, thereby providing a stable growth environment for the mycelium.

[0021] The present invention also provides a method for preparing the aforementioned culture medium, comprising the following steps: mixing the raw materials, adding water and stirring evenly, placing the mixture into bags, sterilizing the mixture at 110-120°C for 1-2 hours, and then cooling the mixture to room temperature. As one possible embodiment, the bag size is 0.8 kg / bag.

[0022] The present invention also provides a cultivation method for Pleurotus geesteranus, comprising the following steps: inoculating the aforementioned culture medium with Pleurotus geesteranus spawn, performing spawning and fruiting management, harvesting the first flush of mature mushrooms, replenishing nutrient solution, and performing spawning and fruiting management again. This method utilizes a "spawning-fruiting-replenishing-reproductive-fruiting" cycle to extend the fruiting cycle of Pleurotus geesteranus and improve the utilization rate of the culture medium.

[0023] In this invention, the spawn management stage refers to the entire process from the inoculation of Pleurotus geesteranus spawn into the bags until the mycelium completely fills the bags and reaches physiological maturity. During this stage, the temperature is preferably 20-25°C, more preferably 22-23°C; the humidity is preferably 75%-85%, more preferably 80%; the lighting conditions are preferably 300-500 lux of scattered light, more preferably 400 lux of scattered light; ventilation is preferably performed in the morning and evening; and disinfection is preferably performed every two days. As an implementation method, the floor of the culture room can be sprayed with disinfectant to prevent contamination by other bacteria.

[0024] In this invention, the fruiting stage refers to the entire process from when the mycelium fills the bag, the bag is opened, to when the fruiting bodies mature and are harvested. The preferred temperature during this stage is 18-24°C, more preferably 20-22°C; and the preferred humidity is 80%-90%, more preferably 85%.

[0025] In the present invention, it is preferred that the cap of the Pleurotus geesteranus fruiting body is not fully unfolded, the edge is slightly curled inward, the color is darker, and the stipe is solid, and it is harvested in time. It is further preferred that residual mycorrhizae be cleaned in time after harvesting to remove rotten and decayed mushrooms.

[0026] In the present invention, the preferred formula of the nutrient solution is: 80-120 g / L of glucose, 8-12 g / L of ammonium sulfate, 4-6 g / L of potassium dihydrogen phosphate, 1.5-3 g / L of magnesium sulfate, 0.5-1.5 g / L of calcium chloride and 0.05-0.2 g / L of vitamin B1; further preferably, it is 90-110 g / L of glucose, 9-11 g / L of ammonium sulfate, 4.5-5.5 g / L of potassium dihydrogen phosphate, 1.8-2.5 g / L of magnesium sulfate, 0.8-1.2 g / L of calcium chloride and 0.08-0.15 g / L of vitamin B1; more preferably, it is 100 g / L of glucose, 10 g / L of ammonium sulfate, 5 g / L of potassium dihydrogen phosphate, 2 g / L of magnesium sulfate, 1 g / L of calcium chloride and 0.1 g / L of vitamin B1. Glucose provides a quickly available carbon source, ammonium sulfate supplements the nitrogen source, potassium dihydrogen phosphate and magnesium sulfate provide phosphorus, potassium, magnesium and other mineral elements, calcium chloride strengthens the cell wall structure of the fruiting body, and vitamin B1 promotes the activity of mycelial metabolic enzymes. The synergistic effect of multiple ingredients can effectively make up for the nutritional loss of the culture medium after the first-stage mushrooms are harvested, and provide sufficient nutrients for the secondary fruiting.

[0027] In the present invention, the preferred method for replenishing the nutrient solution is to soak the fungus bag in the nutrient solution for 10 to 20 minutes to allow the nutrient solution to fully penetrate into the culture medium.

[0028] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0029] Example 1

[0030] A method for cultivating and producing Pleurotus geesteranus comprises the following steps:

[0031] (1) Collection and processing of lychee wood: Fresh lychee wood branches and discarded trunks were collected, and after removing impurities, they were placed in a ventilated and cool place to dry for 5 months, turning them regularly during the period to prevent local rot; after drying, rotten branches were screened and removed, and qualified lychee wood was crushed into sawdust with a particle size of 0.8 cm using a grinder and set aside.

[0032] (2) Preparation of culture medium: Weigh 40 parts of litchi sawdust, 40 parts of cottonseed shells, 17 parts of wheat bran, 1 part of gypsum powder, 1 part of quicklime and 1 part of sucrose by weight, pour them into a blender and mix them evenly; add water while stirring to adjust the moisture content of the culture medium to 62%; put the mixed culture medium into a 0.8 kg / bag fungus bag, compact it and seal it; place the fungus bag in a sterilizer, sterilize it at 115℃ for 1.5 hours, and naturally cool it to room temperature for use.

[0033] (3) Inoculation of Pleurotus geesteranus spawn: In a sterile inoculation room, use aseptic operation to inoculate Pleurotus geesteranus spawn into the sterilized fungus bag. After inoculation, gently press the bag opening and mark it.

[0034] (4) Management of fungus growth: Move the inoculated fungus bags into the culture room and control the ambient temperature to 22°C and the air humidity to 80%; ventilate for 30 minutes each morning and evening to maintain air circulation; adjust the light intensity to 400lx scattered light through the sunshade net; spray the floor of the culture room with disinfectant every 2 days until the mycelium fills the fungus bags.

[0035] (5) Management of mushroom production: After the mycelium has filled the bag, make three oblique cuts along the surface of the bag where the mycelium is dense; adjust the temperature of the culture room to 20℃ and the air humidity to 85%; maintain a stable humidity by spraying humidifier every day to avoid water accumulation at the bag opening.

[0036] (6) Harvesting: When the cap of the Pleurotus geesteranus fruiting body is not fully unfolded, the edge is slightly curled inward, and the stem is solid, use sterile scissors to harvest; after harvesting, clean up the remaining mycorrhizae and rotten mushrooms at the bag opening in time.

[0037] (7) Supplementing nutrient solution to cultivate the second-wave mushroom: prepare nutrient solution according to the formula (glucose 100 g / L, ammonium sulfate 10 g / L, potassium dihydrogen phosphate 5 g / L, magnesium sulfate 2 g / L, calcium chloride 1 g / L, vitamin B1 0.1 g / L); soak the harvested mushroom bags completely in the nutrient solution for 15 minutes, take them out and drain the surface water, and repeat steps (4) to (6) to cultivate the next batch of mushrooms.

[0038] Example 2

[0039] A method for cultivating and producing Pleurotus geesteranus comprises the following steps:

[0040] The difference from Example 1 is that the culture medium is composed of litchi wood shavings 50 parts, cotton seed hulls 30 parts, wheat bran 17 parts, gypsum powder 1 part, quicklime 1 part and cane sugar 1 part by weight fraction.

[0041] Example 3

[0042] A production method of Pleurotus geesteri cultivation, the steps are as follows:

[0043] The difference from Example 1 is that the culture medium is composed of litchi wood shavings 60 parts, cotton seed hulls 20 parts, wheat bran 17 parts, gypsum powder 1 part, quicklime 1 part and cane sugar 1 part by weight fraction.

[0044] Example 4

[0045] A production method of Pleurotus geesteri cultivation, the steps are as follows:

[0046] (1) Collection and treatment of litchi wood: Collect fresh litchi wood branches and discarded trunks, remove impurities and place in a ventilated and cool place for 5.5 months. During this period, turn regularly to avoid local rotting; after air-drying, screen out the rotten branches, and grind the qualified litchi wood into wood shavings with a particle size of 0.6 cm using a grinder, for standby use.

[0047] (2) Preparation of culture medium: Take raw materials according to weight fraction of litchi wood shavings 55 parts, cotton seed hulls 29 parts, wheat bran 10 parts, gypsum powder 2 parts, quicklime 2 parts and cane sugar 2 parts, mix them evenly in a blender; add water while stirring to adjust the water content of the culture medium to 63%; pack the mixed culture medium into mushroom bags with a specification of 0.8 kg / bag, compact and seal; place the mushroom bags in a sterilization pot, sterilize at 110℃ for 2h, and naturally cool to room temperature for standby use.

[0048] (3) Inoculation of Pleurotus geesteri strain: In a sterile inoculation room, use sterile operation to inoculate the Pleurotus geesteri strain into the sterilized mushroom bags, and gently compact the bag opening after inoculation and mark.

[0049] (4) Fungus management: Move the inoculated mushroom bags into the culture room, control the environmental temperature to be 20℃, and the air humidity to be 85%; ventilate for 30 minutes each morning and evening to maintain air circulation; adjust the light to be 300lx scattered light through shading net; disinfect the floor of the culture room by spraying disinfectant every 2 days until the mycelium covers the mushroom bags.

[0050] (5) Mushroom management: After the mycelium covers the mushroom bags, make three diagonal openings along the mycelium dense areas on the surface of the mushroom bags; adjust the temperature of the culture room to 18℃ and the air humidity to 90%; maintain stable humidity by spraying every day to avoid water accumulation at the bag opening.

[0051] (6) Harvesting: Harvest when the cap of the Pleurotus geesteri fruiting body is not fully unfolded, the edge is slightly inwardly rolled, and the stem is solid; clean the residual mycelium and rotten mushrooms at the bag opening after harvesting.

[0052] (7) Supplementing nutrient solution to cultivate the second-wave mushroom: prepare nutrient solution according to the formula (glucose 80 g / L, ammonium sulfate 8 g / L, potassium dihydrogen phosphate 4 g / L, magnesium sulfate 1.5 g / L, calcium chloride 0.5 g / L, vitamin B1 0.05 g / L); soak the harvested mushroom bags completely in the nutrient solution for 10 minutes, take them out and drain the surface water, and repeat steps (4) to (6) to cultivate the next batch of mushrooms.

[0053] Example 5

[0054] A method for cultivating and producing Pleurotus geesteranus comprises the following steps:

[0055] (1) Collection and processing of lychee wood: Collect fresh lychee wood branches and discarded trunks, remove impurities and place them in a ventilated and cool place to dry for 6 months, turning them regularly during the period to prevent local rot; after drying, screen and remove rotten branches, and crush qualified lychee wood into sawdust with a particle size of 1.0 cm using a grinder for later use.

[0056] (2) Preparation of culture medium: Weigh 45 parts of litchi sawdust, 30.5 parts of cottonseed shells, 20 parts of wheat bran, 1.5 parts of gypsum powder, 1.5 parts of quicklime and 1.5 parts of sucrose by weight, pour them into a blender and mix them evenly; add water while stirring to adjust the moisture content of the culture medium to 65%; put the mixed culture medium into a 0.8 kg / bag fungus bag, compact it and seal it; place the fungus bag in a sterilizer, sterilize it at 120°C for 1 hour, and naturally cool it to room temperature for use.

[0057] (3) Inoculation of Pleurotus geesteranus spawn: In a sterile inoculation room, use aseptic operation to inoculate Pleurotus geesteranus spawn into the sterilized fungus bag. After inoculation, gently press the bag opening and mark it.

[0058] (4) Management of fungus growth: Move the inoculated fungus bags into the culture room and control the ambient temperature to 25°C and the air humidity to 75%; ventilate for 30 minutes each morning and evening to maintain air circulation; adjust the light intensity to 500lx scattered light through the sunshade net; spray disinfectant on the floor of the culture room every 2 days until the mycelium fills the fungus bags.

[0059] (5) Management of mushroom production: After the mycelium has filled the bag, make three oblique cuts along the surface of the bag where the mycelium is dense; adjust the temperature of the culture room to 24°C and the air humidity to 80%; maintain a stable humidity by spraying humidifier every day to avoid water accumulation at the bag opening.

[0060] (6) Harvesting: When the cap of the Pleurotus geesteranus fruiting body is not fully unfolded, the edge is slightly curled inward, and the stem is solid, use sterile scissors to harvest; after harvesting, clean up the remaining mycorrhizae and rotten mushrooms at the bag opening in time.

[0061] (7) Cultivation of second flush of mushrooms with supplementary nutrient solution: Prepare the nutrient solution according to the formula (glucose 120 g / L, ammonium sulfate 12 g / L, potassium dihydrogen phosphate 6 g / L, magnesium sulfate 3 g / L, calcium chloride 1.5 g / L, vitamin B1 0.2 g / L); completely immerse the harvested mushroom bags in the nutrient solution for 20 min, drain the surface water after taking out, and repeat steps (4) to (6) to cultivate the next batch of mushrooms.

[0062] Test Example 1

[0063] Set up Comparative Examples 1 to 3, replace the litchi wood chips in the Pleurotus geesteri culture medium of Examples 1 to 3 with an equal amount of cotton seed hulls, and measure the mycelial growth rate and single fruit weight.

[0064] Measurement standards:

[0065] (1) Mycelial growth rate: randomly select 3 bags of mushroom bags for each test treatment group, draw a line from the shoulder of the bag with a marker pen when the mycelium starts to grow, draw a straight line from the front of the mycelium growth after 7 days, measure the length of each 7 days with a ruler, calculate the mycelial growth rate, and take the average value.

[0066] (2) Single fruit weight: randomly select 3 fruit bodies of the first flush of mushrooms for each test treatment group and weigh them, and take the average value.

[0067] Test results:

[0068] As shown in Table 1, there is a significant difference in the effect of different culture media on the mycelial growth rate of Pleurotus geesteri. The average mycelial growth rate of Example 2 is 3.47 cm / 7d, which is 7.4% higher than that of Comparative Example 2 (3.23 cm / 7d); the average mycelial growth rate of Example 3 is 3.60 cm / 7d, which is 8.1% higher than that of Comparative Example 3 (3.33 cm / 7d), indicating that medium-high proportion of litchi wood chips can significantly promote mycelial growth. There is no difference in the mycelial growth rate between Example 1 and Comparative Example 1, indicating that a small amount of cotton seed hulls replacing litchi wood chips has no obvious effect on the mycelial growth rate of Pleurotus geesteri, but there is still an advantage of recycling litchi wood resources and optimizing costs.

[0069] Table 1 Effect of different culture media on mycelial growth rate of Pleurotus geesteri

[0070]

[0071] As shown in Table 2, the influence trend of different culture media on the weight of a single mushroom of Pleurotus geesteranus is consistent with the mycelial growth rate. The average weight of a single mushroom in Example 2 is the highest, reaching 45.691g, which is 28.3% higher than the 35.608g of Comparative Example 2, and the fruiting body yield advantage is significant; the average weight of a single mushroom in Example 3 is 40.721g, which is 8.3% higher than the 37.588g of Comparative Example 3, indicating that litchi sawdust can effectively increase the weight of a single mushroom. The average weight of a single mushroom in Comparative Example 1 is slightly higher than that in Example 1, but the difference is mainly caused by the abnormally high single value (54.091g) in Comparative Example 1. The actual data fluctuates greatly, while the data of the embodiment group is more stable, indicating that the culture media of the present invention can provide more balanced nutritional conditions for the growth of fruiting bodies and reduce yield fluctuations.

[0072] Table 2 Effects of different culture media on the weight of a single flower of Pleurotus geesteranus

[0073]

[0074]

[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A Pleurotus geesteranus culture medium, characterized in that: The preparation method comprises the following raw materials in parts by weight: 40-60 parts of litchi sawdust, 20-40 parts of cottonseed shells, 10-20 parts of wheat bran, 1-2 parts of gypsum powder, 1-2 parts of quicklime and 1-2 parts of sucrose.

2. The culture medium according to claim 1, characterized in that The lychee sawdust is made by drying fresh lychee wood in the shade for 5 to 6 months, removing rotten branches, and then crushing the wood into pieces of 0.6 to 1 cm.

3. The culture medium according to claim 1, characterized in that The water content is 62% to 65%.

4. The method for preparing the culture medium according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: mixing various raw materials, adding water and stirring evenly, putting the raw materials into fungus bags, sterilizing at 110-120 DEG C for 1-2 hours, and cooling to room temperature.

5. Use of the culture medium according to any one of claims 1 to 3 in the cultivation of Pleurotus geesteranus.

6. A method for cultivating and producing Pleurotus geesteranus, characterized in that: The method comprises the following steps: inoculating the culture medium according to any one of claims 1 to 3 with the Pleurotus geesteranus strain, performing spawning and fruiting management, harvesting the first-batch mushrooms after they mature, replenishing nutrient solution, and performing spawning and fruiting management of the Pleurotus geesteranus again.

7. The cultivation production method according to claim 6, characterized in that: During the spawn management stage, the temperature is 20-25° C., the air humidity is 75%-85%, the lighting condition is 300-500 lx scattered light, and ventilation is carried out in the morning and evening.

8. The cultivation production method according to claim 6, characterized in that: During the mushroom production management stage, the temperature is 18-24° C. and the air humidity is 80%-90%.

9. The cultivation production method according to claim 6, characterized in that: The formula of the nutrient solution is: 80-120 g / L of glucose, 8-12 g / L of ammonium sulfate, 4-6 g / L of potassium dihydrogen phosphate, 1.5-3 g / L of magnesium sulfate, 0.5-1.5 g / L of calcium chloride and 0.05-0.2 g / L of vitamin B1.

10. The cultivation production method according to claim 6 or 9, characterized in that: The nutrient solution is supplemented by soaking the fungus bag in the nutrient solution for 10 to 20 minutes to allow the nutrient solution to fully penetrate into the culture medium.

Images