A method for cultivating a phalaenopsis

Abstract

The present application relates to the field of flower cultivation technology, and discloses a kind of cultivation methods of butterfly orchid, comprising: before low temperature induction treatment, by analyzing the gas metabolism response characteristics of plant population in diagnostic dark environment, it is admitted to check;After checking, controlled metabolic consumption is executed to plant population, and in the period, osmotic stress pulse is applied to synchronize root activity, then energy accumulation is synchronized;The present application carries out systematic physiological function regulation including admission check to plant population, and transforms passive adaptation to biological individual difference in traditional cultivation into active and whole plant dimension physiological state standardization, so that the certainty of cultivation process is fundamentally changed from biological randomness to industrial process control, and the predictability and consistency of production are improved.

Description

Technical Field

[0001] This invention relates to a method for cultivating Phalaenopsis orchids, belonging to the field of flower cultivation technology. Background Technology

[0002] In current commercial production practices of Phalaenopsis orchids, in order to meet the concentrated supply demand of specific holiday markets, a standardized linear production process is generally followed. This process involves allowing the plant to accumulate the necessary biomass through long-term vegetative growth, followed by uniform induction treatment in a low-temperature environment, eventually leading to flowering. This cultivation method has provided fundamental technical support for the rapid development of the Phalaenopsis orchid industry over the past few decades and has become the industry's recognized mainstream operating procedure.

[0003] However, with the rapid expansion of production scale and the increasing market demand for product quality uniformity, an inherent limitation of the aforementioned classic cultivation method—its long-term passive acceptance—has begun to emerge. This is because the inherent individual differences within the biological population mean that even under strictly uniform management conditions, after experiencing the same vegetative growth period, different plants exhibit a wide statistical distribution in the levels of carbohydrate accumulation and key enzyme activity used to support flower bud differentiation. When this group, whose internal physiological state is still discrete, is placed under a uniform low-temperature signal, the response speed and ability of each plant to this signal will differ, directly leading to inconsistent flowering times, varying flower stalk numbers, and inconsistent flower quality. To address this problem, production enterprises have to invest significant manpower in continuous manual sorting, batch reorganization, and differentiated management. This not only significantly increases the complexity and management costs of the production process but also fundamentally restricts the yield and ultimate economic benefits.

[0004] To alleviate this problem, some tentative improvement ideas have emerged in the industry, such as attempting to compensate for differences by providing additional lighting or fertilization to slower-growing individuals. However, this catch-up management approach is not only impractical in production scales of tens of thousands of plants, but its effectiveness is also difficult to guarantee due to individual differences in plant absorption capacity. Analysis reveals that the fundamental dilemmas of existing technologies are: 1. The lack of a pre-treatment step that can effectively eliminate or mitigate inherent individual differences in the organisms before applying the crucial and uniform flowering induction signal to the entire population; 2. All subsequent remedial management methods attempt to level off the differences within an already differentiated population, which is logically more complex and costly than downward unification, failing to address the problem of inconsistent initial states. Therefore, the technical problem this invention aims to solve is how to establish a low-cost, scalable method that can effectively pre-treat the internal physiological state of the entire Phalaenopsis orchid cultivation population before the crucial flowering induction process begins, avoiding uncertainties in subsequent production processes caused by individual differences. Summary of the Invention

[0005] This invention provides a method for cultivating Phalaenopsis orchids, the main purpose of which is to solve the problems of asynchronous flowering, low yield and complicated management caused by the lack of a pre-standardized treatment of the physiological state of the biological population in existing cultivation methods.

[0006] To achieve the above objectives, the present invention provides a method for cultivating Phalaenopsis orchids, comprising, firstly, performing a population health status verification step after the end of the vegetative growth period of the Phalaenopsis orchid plants and before the low-temperature induction treatment, obtaining the health status of the plant population within a closed environment caused by metabolic activity during a diagnostic dark environment pulse. The concentration change characteristics are then analyzed; it is then determined whether the change characteristics meet the admission criteria defined by historical data from a healthy population; only when the change characteristics meet the admission criteria are subsequent metabolic reset and root synchronization treatments performed, wherein the metabolic reset and root synchronization include:

[0007] Step a, perform a controlled metabolic depletion treatment, which includes placing the plant population under light conditions below its light compensation point for 5 to 7 consecutive days and completely stopping fertilization.

[0008] Step b, during the duration of the controlled metabolic consumption treatment, at least one brief osmotic stress pulse is synergistically applied to the cultivation substrate of the plant population to perform root activity synchronization treatment.

[0009] Step c: Within 24 hours after the controlled metabolic consumption treatment, a synchronized energy accumulation treatment is performed, which includes providing saturated light conditions and high phosphorus and potassium fertilizer for 2 to 3 days; after the synchronized energy accumulation treatment is completed, the plant population is subjected to a low temperature induction treatment.

[0010] Preferably, the population health status verification step, before judging the change characteristics, further includes: determining a dynamic health benchmark for comparison with the change characteristics based on one or more current environmental parameters and growth stage parameters of the plant population; wherein the one or more current environmental parameters include temperature, and the growth stage parameters include the number of cultivation days.

[0011] Preferred, The characteristics of concentration changes, through the analysis of The coefficient of determination is calculated by linear regression fitting of the concentration-time curve. Characterization is performed; admission criteria are: ,in, This is a threshold determined by analyzing historical data of a healthy population and used to characterize the homogeneity of the population.

[0012] Preferably, when the change feature does not meet the admission criteria, but the change feature corresponds to... When the average rate of change of concentration meets the activity conditions calibrated by historical data of healthy population, the plant population is subjected to a period of low light treatment before performing metabolic reset and root synchronization treatment. After the low light treatment is completed, the population health status verification step is performed again.

[0013] Preferably, the illumination conditions in step a below its light compensation point are specifically photosynthetically active radiation intensities of 5 to 15. .

[0014] Preferably, the high phosphorus and potassium fertilizer provided in step c has a nitrogen, phosphorus and potassium element mass ratio of 1:2:1.5 to 1:3:2.

[0015] Preferably, the osmotic pressure stress pulse applied in step b is achieved by applying a solution containing neutral salt through the irrigation system.

[0016] Preferably, the neutral salt is potassium sulfate, with a concentration of 50 to 150 mg / L in the solution, and each application lasts for 30 to 60 minutes.

[0017] Preferably, the application of a diagnostic dark environment pulse specifically involves subjecting the plant population to complete darkness for 1 to 2 hours after the daytime light exposure has ended.

[0018] Preferably, the low-temperature induction treatment involves lowering the temperature of the environment in which the plant population is located to 16 to 20 degrees Celsius.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. This cultivation method involves subjecting the plant population to controlled metabolic consumption before low-temperature induction treatment. By using light conditions below the light compensation point and ceasing fertilization, the internal physiological state of different plants, regardless of their initial energy reserves, converges to a uniform low-level baseline. Subsequently, in the synchronized energy accumulation treatment, all plants starting from the same baseline produce a consistent absorption and accumulation response to the uniformly provided saturated light and nutrients. This process transforms the passive adaptation and later adjustment to individual differences in traditional cultivation methods into the pre-standardization of the physiological state of the entire biological population before the start of key processes. This means that the subsequent uniform low-temperature induction treatment no longer deals with a population with a discrete distribution of physiological states, but with a collection of standardized components similar to those in industrial production, where initial conditions are uniform. The determinism of the production process is therefore no longer subject to the inherent randomness of the organism.

[0021] 2. Before the metabolic reset treatment begins, this method adds a step to verify the health status of the plant population. This involves applying a diagnostic dark environment pulse in a closed environment and obtaining data on the effects of population metabolic activity within the closed environment. The characteristics of concentration changes. This step transforms a macroscopic, easily measurable gaseous parameter in the environment into a direct, non-invasive characterization of the microscopic physiological state of the population. Furthermore, it allows for the analysis of this... Morphological analysis of the concentration change curves revealed the homogeneity of physiological states within the population. Based on this, the method selects from multiple preset treatment paths. This means that the initiation of the cultivation process is no longer based on fixed time points or experience judgments, but on decisions based on the current real and global physiological snapshot of the production object. This avoids the risk of introducing unsuitable populations into the consumable treatment process and allows for targeted pre-conditioning of populations with discrete states.

[0022] 3. When verifying the health status of a population, the method can also dynamically determine the preset health benchmarks used for comparison based on current environmental parameters and plant growth stage parameters. For example, based on the real-time greenhouse temperature, the corresponding healthy respiration rate benchmark can be calibrated. This mechanism makes the diagnostic scale no longer fixed, but can adapt to environmental changes and the plant's own growth process in real time, avoiding false negatives or false positives caused by environmental fluctuations or natural growth of plant biomass. This ensures that the aforementioned verification steps maintain their reliability and impartiality in the ever-changing real agricultural environment throughout the entire long production cycle. Furthermore, during controlled metabolic consumption treatment of the aboveground parts of the plant, this method can also be used in conjunction with... By applying a brief and non-harmful osmotic pressure stress pulse to the cultivation substrate, the lower metabolic activity of the aboveground parts creates specific physiological conditions for the root system to respond to external stimuli. This osmotic pressure pulse can induce synchronized compensatory stress germination in the plant roots. As a result, when cultivation enters the subsequent synchronized energy accumulation stage, not only is the energy reserve state of the aboveground parts of the plant population uniform, but the absorption activity of its underground roots also reaches a peak simultaneously. Through this coupling of aboveground and underground treatment steps, the method expands the object of synchronization from a single energy reserve state to energy acquisition capacity, achieving synergistic consistency of physiological functions across the entire plant. This ensures that when fertilizer and water are supplied uniformly, each plant can absorb and transform nutrients with uniform efficiency. Attached Figure Description

[0023] Figure 1 This is a flowchart illustrating the cultivation method of Phalaenopsis orchids according to the present invention.

[0024] Figure 2 For the healthy homogeneous group and discrete group of the present invention Comparison of concentration change curves;

[0025] Figure 3 This is a schematic diagram illustrating the principle of standardized regulation of the physiological state of plant populations in this invention.

[0026] The objectives, features, and advantages of this invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be described in detail below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0028] This invention provides a method for cultivating Phalaenopsis orchids. The method includes a physiological function regulation stage for the plant population after the end of the vegetative growth period and before the low-temperature induction treatment. This stage includes, in sequence, a population health status verification step, a metabolic reset and root synchronization treatment step, and a synchronized energy accumulation treatment step. The population health status verification step is used to determine whether the plant population is suitable for subsequent regulation processes. The metabolic reset and root synchronization treatment step is used to achieve synergistic unity between the energy reserves of the above-ground parts and the absorption capacity of the underground parts of the plant. The synchronized energy accumulation treatment step is used to uniformly raise the standardized plant population to a physiological state suitable for flowering induction.

[0029] In the large-scale cultivation of Phalaenopsis orchids, to ensure the synchronicity of flowering induction treatment, it is necessary to standardize the physiological state of the plant population. However, before implementing low-temperature induction, there is a lack of effective means to assess and unify the physiological differences within the population. Therefore, this cultivation method includes a population health status verification step to determine whether the cultivation population is suitable for subsequent consuming treatments without destructive sampling. In a specific application, this verification step is performed as follows: After daytime light exposure, first ensure the greenhouse environment is sealed, then apply a diagnostic dark environment pulse (i.e., complete darkness treatment) to the plant population for a duration of 1 to 2 hours; during this period, carbon dioxide (CO2) is introduced into the greenhouse environment... The sensor continuously records the respiratory and metabolic activities of the plant population within a closed environment at a set sampling frequency. Concentration variation data; a healthy and homogeneous plant population with relatively consistent respiration rates, from which data were collected. The concentration-time curve will morphologically approximate a straight line with a stable slope, while in groups with highly differentiated internal physiological states, the curve will exhibit non-linear characteristics. Therefore, by analyzing this... The concentration-time curve was fitted with linear regression, and its coefficient of determination was calculated. This allows for the acquisition of a quantitative index that characterizes the homogeneity of a group, thereby enabling a non-invasive assessment of the homogeneity of physiological states within the group.

[0030] To ensure the accuracy of the above verification judgments is not affected by fluctuations in environmental conditions, the population health status verification step further includes a mechanism for determining a dynamic health baseline. The basal respiration rate of the plant population is affected by ambient temperature and its total biomass (which can be characterized by the number of cultivation days). Therefore, in the initial deployment phase, this method establishes a parameterized model describing the relationship between the respiration rate of a healthy plant population and temperature and the number of cultivation days through calibration experiments. For example, healthy respiration rate = f(temperature, number of cultivation days). Before each diagnostic dark environment pulse is executed, the control system obtains the current ambient temperature from the temperature sensor and, combined with the current number of cultivation days, instantly generates a dynamic health baseline that matches the current state by calling the aforementioned model. Correspondingly, the admission condition for population homogeneity is determined by comparing the real-time calculated coefficient of determination. Is it greater than or equal to a preset uniformity threshold? For example, if the system presets a threshold The value is 0.95, when the real-time calculated value is... If the group is deemed to be in a uniform state, it is allowed to proceed to the subsequent processing steps; this mechanism will solidify a fixed state.

[0031] Subsequent metabolic reset and root synchronization treatments are performed only after the change characteristics meet the admission criteria. This treatment aims to converge plant populations with differing initial states to a unified low-energy physiological baseline and synchronize their root activity. Step a is a controlled metabolic consumption treatment, which involves adjusting the greenhouse lighting system to maintain the photosynthetically active radiation intensity of the plant population's environment at 5 to 15 ppm for 5 to 7 consecutive days. Within this range, the light intensity is below the light compensation point of the Phalaenopsis orchid, resulting in a negative net energy income for the plant, consuming its internally stored carbohydrates. During this period, fertilization is completely stopped. The duration of this treatment is based on the fact that 5 days allows plants with moderate energy reserves to reach a low metabolic level, while 7 days allows plants with initially abundant energy reserves to consume their accumulated reserves, without causing physiological damage to healthy plants. During the duration of the aforementioned controlled metabolic consumption treatment, step b is performed concurrently, namely, applying at least one brief osmotic stress pulse to the cultivation substrate of the plant population through the irrigation system. This operation specifically involves using a solution containing neutral salts. Short-term irrigation with potassium sulfate as the neutral salt, at a concentration controlled between 50 and 150 mg / L, was applied for 30 to 60 minutes each time. This concentration range was set because below 50 mg / L, the resulting osmotic pressure was insufficient to provide effective physiological stimulation, while above 150 mg / L, it could cause salt stress damage to the roots. Therefore, this concentration window was designed to induce compensatory stress germination in the roots without causing damage. Because the aboveground metabolic activity of the plant is weak at this time, and the roots are sensitive to changes in the external environment, this stress pulse can induce the entire plant to synchronously germinate new capillary roots, thus ensuring consistent absorption capacity in the subsequent nutrient absorption stage.

[0032] After the controlled metabolic consumption treatment, to transform the plant population from a standardized low-energy consumption state to a high-energy state suitable for flowering, step c, namely the synchronized energy accumulation treatment, is then performed. This treatment is initiated within 24 hours after the completion of the metabolic consumption treatment. Specifically, the greenhouse environment is restored to suitable growth conditions, saturated light is provided, and high-phosphorus and potassium fertilizer is applied for 2 to 3 days. The nitrogen-phosphorus-potassium mass ratio of the high-phosphorus and potassium fertilizer is in the range of 1:2:1.5 to 1:3:2. This ratio is set based on the fact that phosphorus promotes flower bud differentiation and potassium promotes carbohydrate metabolism. The synthesis and transport of nitrogen are controlled, while the proportion of nitrogen is appropriately reduced to inhibit vegetative growth. After 2 to 3 days of accumulation, the energy levels of all plants starting from the same low baseline are synchronously raised to a suitable and uniform flowering plateau. At this point, the physiological function regulation stage is completed, and the plant population can then be subjected to low-temperature induction treatment, for example, lowering the temperature of its environment to 16 to 20 degrees Celsius to initiate the synchronized flowering process. In addition, this cultivation method also includes procedures for dealing with special situations, namely, when the judgment result of the population health status verification step is that the change characteristics do not meet the uniformity admission conditions (e.g. However, the value calculated from this change characteristic When the average rate of change in concentration meets the activity conditions calibrated by historical data of the healthy population, a prolonged period of low-light treatment is applied to the plant population before performing metabolic reset and root synchronization treatments. This preconditioning procedure is used to preferentially deplete the reserves of plants with excessively high energy reserves in the population, bringing their state closer to that of plants with lower reserves, thereby reducing the dispersion of the entire population. After the low-light treatment is completed, the population health status verification step is repeated until the homogeneity of the population meets the admission criteria before entering the standard treatment process. This adaptive conditioning path improves the applicability of this method to populations in different initial states.

[0033] Example 1: The cultivation method of the present invention was applied in a large commercial Phalaenopsis orchid production greenhouse for the Spring Festival market supply. The greenhouse cultivated a batch of 50,000 Phalaenopsis orchid plants that had completed vegetative growth. The plants in this batch were in good growth condition and similar in size. However, the energy reserves used to support flowering were discretely distributed due to inherent individual differences. If a uniform cooling method was used directly, the flowering time would be inconsistent, which would not be able to meet the concentrated market demand of a specific festival.

[0034] Before the scheduled low-temperature induction treatment date for this batch of plants, a population health status verification procedure was initiated; after the daytime light exposure ended, the greenhouse was sealed, and a diagnostic dark environment pulse lasting 2 hours was applied to the plant population; during this period, the environmental data were recorded by the carbon dioxide sensor inside the greenhouse. Concentration data show that The concentration-time curve exhibits a non-linear relationship; the coefficient of determination was calculated after linear regression fitting. The value is 0.88, which is lower than the system's preset uniformity threshold. The coefficient of determination (COD) was 0.95, but its average rate of change met the activity condition, indicating that the population was generally healthy but had a high degree of dispersion in its internal physiological state. Based on this judgment, the system did not directly proceed to metabolic consumption treatment, but instead automatically executed a two-day low-light treatment preconditioning program to reduce the differences in physiological state within the population. After the preconditioning was completed, the population health status verification step was executed again, and the coefficient of determination obtained this time was... The value is 0.97, which meets the entry requirements, and the cultivation process proceeds to the next stage.

[0035] Subsequently, the system automatically began metabolic reset and root synchronization treatment; the greenhouse's shading system was activated, reducing and maintaining the photosynthetically active radiation intensity in the environment at 10. Simultaneously, the water and fertilizer system stopped all fertilization, and this controlled metabolic consumption treatment lasted for 6 days. On the 3rd and 5th days of this period, the irrigation system implemented a brief osmotic stress pulse, applying a potassium sulfate solution with a concentration of 100 mg / L to the cultivation substrate, each lasting 45 minutes. Among these, the low light treatment of the aboveground parts weakened the plant's metabolic activity, creating specific physiological conditions for the root system to respond to external stimuli. Under these conditions, the applied osmotic pressure pulse could effectively induce synchronized stress germination in the roots, thereby achieving synergistic regulation of both the energy state of the aboveground parts and the absorption capacity of the underground parts.

[0036] After a 6-day metabolic consumption treatment, the process immediately transitioned to a synchronized energy accumulation treatment. The greenhouse shading system was shut down, and the supplemental lighting system was activated to provide saturated light. Simultaneously, the water and fertilizer system began applying a high-phosphorus-potassium fertilizer with a nitrogen-phosphorus-potassium ratio of 1:2.5:2, a process that lasted for 3 days. Since all plants started from a standardized low-energy baseline and synchronized root vitality state, they exhibited consistent absorption and conversion efficiency in response to uniformly supplied light and nutrients. This approach of first unifying the physiological state of the population to the same baseline and then synchronously improving it avoids the complexity and uncertainty encountered in traditional methods that attempt to catch up with an already differentiated population.

[0037] Finally, after completing the synchronization energy product treatment, the batch of 50,000 plants were transferred to a low-temperature environment of 18 degrees Celsius for uniform induction treatment. Subsequently, the flower stalk emergence and flowering process of the entire group showed a high degree of consistency, with a flowering synchronization rate of over 95% and a defect rate of less than 3%. The entire batch was put on the market within a 10-day window before the Spring Festival, eliminating the need for extensive manual sorting and batch reorganization. The predictability of the production process and the efficiency of resource utilization were improved.

[0038] Example 2: To objectively verify the actual effect of the cultivation method of the present invention on the flowering uniformity and finished product quality of Phalaenopsis orchids, a set of comparative experiments was set up; the experiment was conducted in two independent greenhouse compartments with the same specifications and environmental control systems, each compartment being equipped with a system that could adjust the photosynthetically active radiation intensity with an accuracy of ±2 The supplemental lighting system, and the environmental control unit that can control temperature fluctuations within ±0.5 degrees Celsius.

[0039] The experimental material consisted of 1000 healthy Phalaenopsis orchid seedlings of the same strain, uniform in age and size, randomly divided into a control group and an experimental group, with 500 seedlings in each group. Each group was placed in one of the two greenhouse compartments. Both groups were cultivated under the same standard vegetative growth conditions before the experiment began. The control group underwent conventional production methods, i.e., after completing the vegetative growth period, the ambient temperature was uniformly lowered to 18 degrees Celsius for low-temperature induction. The experimental group, before entering low-temperature induction, underwent the physiological function regulation method of this invention, with the key process parameters set as follows: a controlled metabolic consumption treatment for 6 days, during which the photosynthetically active radiation intensity was maintained at 10... On day 3 and day 5, an osmotic stress pulse of potassium sulfate solution at a concentration of 100 mg / L was applied, followed by a 3-day synchronized energy accumulation treatment. After the treatment was completed, the ambient temperature was lowered to 18 degrees Celsius, the same as the control group.

[0040] After being transferred to low-temperature induction treatment, the flowering progress and quality indicators of the two groups of plants were continuously statistically analyzed, and the results showed significant differences. Regarding the synchronization rate of initial flowering, the experimental group reached 95.6%, while the control group only achieved 68.2%. This improved consistency was directly reflected in the peak flowering period; the experimental group entered peak flowering on the 65th day after induction, 13 days earlier than the control group's 78th day. In terms of final product quality, the experimental group achieved a first-grade product rate of 88.5%, far exceeding the control group's 61.4%. Correspondingly, the rejection rate in the experimental group decreased from 15.8% in the control group. The difference in these figures is as follows: before the low-temperature induction treatment, the experimental group effectively unified the baseline of the physiological state within the population through metabolic reset and root synchronization treatment, enabling the entire population to produce a more consistent and efficient response to the subsequent unified induction signal. The experimental results show that, compared with the existing conventional cultivation methods, the method of this invention can transform the flowering process of the cultivation population into a production process with improved predictability and consistency, which has a positive effect on improving the yield of commercial production and meeting the supply demand during specific market windows.

[0041] Example 3: This example combines Figures 1 to 3 This document describes the implementation of a cultivation method for Phalaenopsis orchids. For example... Figure 1 As shown, the process begins with the administrator initiating the cultivation process. The control system then activates sensors to monitor the environment and instructs the greenhouse environment to be sealed. Next, a dark environment pulse is applied for 1 to 2 hours. During this period, the respiratory and metabolic activities of the plant population cause changes in the greenhouse environment. Changes in concentration are fed back to the control system in real time by sensors, and the control system calculates these changes. coefficient of determination of concentration change curve It then compares this value with a preset threshold of 0.95 to perform a conditional judgment. At this point, the process enters the main path of the alternative treatment sequence indicated by alt, which involves sequentially performing a 5- to 7-day metabolic depletion treatment, during which osmotic stress pulses are applied in conjunction, followed by a 2- to 3-day energy accumulation treatment, ultimately reducing the ambient temperature to 16-20 degrees Celsius. To enter the flowering induction stage, and when If the light is low, the system will enter the backup path of the alternative processing sequence. This means that weak light preprocessing will be performed first, and the health check step will be re-executed after the processing is completed until the check passes before entering the main processing path.

[0042] like Figure 2 As shown, the closed environment corresponding to the healthy homogeneous group (solid line) and the discrete group (dashed line) during the diagnostic dark environment pulse. A comparison of typical curves showing concentration changes over time (minutes). The horizontal axis represents the time progression from the start of dark environment treatment to 120 minutes, and the vertical axis represents the concentration in the environment. Concentration (ppm), where the concentration of a healthy, homogeneous population is indicated by dots. The concentration change curve exhibits a highly linear characteristic, and its linear regression fit has a determination coefficient of [missing information]. The value is 0.98, while the discrete population with triangular labels... The concentration change curve exhibits significant nonlinear fluctuations, and its coefficient of determination... With a value of only 0.88, this figure visually demonstrates how to quantitatively assess and differentiate plant populations with different physiological homogeneity levels by analyzing the morphological characteristics of macroscopic environmental gas parameter curves.

[0043] like Figure 3 As shown, in the initial state, the population consists of a mixture of plants with varying energy reserves (high-energy-reserve plants) represented by different filling patterns, and their physiological states are discrete, corresponding to... When the value is less than 0.95, after metabolic reset treatment, the physiological state of all plants (low baseline after metabolic consumption) is unified to a uniform low baseline level represented by a dashed box. At this point, the population state converges. After subsequent energy accumulation treatment, all plants starting from the same baseline consistently reach a uniform high-level energy state (high level after energy accumulation) represented by a densely filled pattern. The value can reach a level greater than or equal to 0.97. Finally, when a flowering induction signal is applied to this highly homogeneous population, its flowering process is highly synchronized (synchronous flowering marker), achieving a synchronization rate of over 95% and a first-grade product rate of over 88%. This figure reveals the core technical logic of this invention, which first converges to a unified baseline and then synchronizes and improves the entire process of transforming a biologically random population into a physiologically standardized industrial production object.

[0044] Example 4: In a specific application, this cultivation method needs to be applied to a newly introduced Phalaenopsis orchid variety with unknown physiological stress response characteristics. To determine the appropriate concentration of neutral salt for this variety under osmotic stress pulses, the following calibration procedure was established: 300 Phalaenopsis orchids of uniform growth status and size belonging to this new variety were selected as calibration subjects and randomly divided into 6 experimental groups, with 50 plants in each group. All groups were placed in multiple small growth chambers with independently controllable environmental parameters. The environmental control system of the growth chambers could independently maintain the light and temperature conditions required for subsequent processes. Before the calibration process began, all groups underwent routine vegetative growth under consistent conditions. After the calibration process started, all 6 groups were uniformly subjected to a 7-day controlled metabolic consumption treatment, during which the photosynthetically active radiation intensity was kept constant at 12. And completely stop fertilizing.

[0045] On the fourth day of the treatment, six sample groups were subjected to a 45-minute osmotic stress pulse of potassium sulfate solution at different concentrations. Sample group 1 served as a blank control group with an applied solution concentration of 0 mg / L; sample groups 2 to 6 served as gradient experimental groups with applied solution concentrations of 40 mg / L, 80 mg / L, 120 mg / L, 160 mg / L, and 200 mg / L, respectively. 72 hours after the stress pulse ended, 10 plants were randomly selected from each sample group. Two quantitative evaluation indicators were statistically analyzed using image acquisition and analysis: the average number of newly generated healthy root tips per plant, to characterize the effectiveness of stress germination; and the damage rate of necrosis or browning symptoms at the root tips, to characterize the negative effects of stress.

[0046] Statistical results showed that the average number of newly formed healthy root tips in the blank control group and sample group 2 (concentration 40 mg / L) did not increase significantly compared with the control group; the average number of newly formed healthy root tips in sample groups 3 and 4 (concentrations 80 mg / L and 120 mg / L), respectively, was 3.5 times and 4.2 times that of the control group, and the root damage rate was less than 1% in both groups; the number of newly formed healthy root tips in sample group 5 (concentration 160 mg / L) did not increase further compared with sample group 4, but the root damage rate increased to 5%; the root damage rate in sample group 6 (concentration 200 mg / L) was significantly higher than that in sample group 4. The damage rate rose to over 15%. Based on the data from the above calibration test, the applicable concentration range of potassium sulfate solution in the osmotic stress pulse for this new Phalaenopsis variety was determined to be 80 mg / L to 120 mg / L. In subsequent large-scale production, the standard operating procedure for this variety was set to 100 mg / L. This value can achieve a root germination stimulation effect close to that at a concentration of 120 mg / L, while its damage risk is much lower than that at a concentration level of 160 mg / L. Thus, the operating parameters of this technical solution for this specific application were determined.

[0047] Example 5: In a specific deployment scenario, when this cultivation method is first applied to a new production greenhouse or a Phalaenopsis orchid variety without data accumulation, a parameterized baseline model calibration procedure needs to be performed before large-scale application. This procedure is used to establish a dynamic health baseline that matches the specific variety and environmental conditions for subsequent population health status verification steps.

[0048] The calibration procedure is performed as follows: First, a batch of representative plants of the target variety with healthy physiological conditions, consistent growth stages, and belonging to the same physiological stage are selected and placed in a test chamber where environmental parameters can be precisely controlled. For a period covering the plant's growth stage from late vegetative growth to pre-flowering, the ambient temperature within the test chamber is systematically graded, varying in 1-degree Celsius increments within a range of 15 to 30 degrees Celsius. At each stable temperature point, a standardized diagnostic dark environment pulse is executed daily at a set time. Simultaneously, a carbon dioxide sensor is used to record the changes in the closed environment caused by the plant's collective respiration. The average rate of change of concentration; in this way, the system collects and stores a series of three-dimensional data points including ambient temperature, number of cultivation days, and the corresponding population healthy respiratory rate.

[0049] After completing the above data collection, mathematical statistics methods such as multiple regression analysis were used to perform surface fitting on the obtained three-dimensional data points, thereby establishing a functional relationship that can describe the healthy respiratory rate and the environmental temperature and number of cultivation days: Healthy respiratory rate = A parameterized baseline model (temperature, number of cultivation days) is generated, or a high-resolution lookup table is generated. This model or lookup table is then embedded into the central control system of the production greenhouse, serving as the basis for generating dynamic health baselines when verifying the health status of all subsequent batches. This ensures that the baselines used in the verification process can reflect the physiological characteristics of the specific production environment and the cultivated objects.

[0050] Example 6: In an application for establishing a standardized production baseline for a new Phalaenopsis orchid variety, a preliminary control parameter matrix calibration procedure needs to be performed to accurately adapt this cultivation method to the specific physiological characteristics of the variety. The first part of this procedure aims to determine the optimal duration of the controlled metabolic consumption treatment, which is performed by selecting a batch of healthy plants of the new variety and subjecting them to a uniformly controlled photosynthetically active radiation intensity of 12... Following the controlled metabolic consumption treatment, the depletion of the plant's internal energy reserves was monitored every 24 hours by analyzing the soluble sugar content of randomly selected leaf samples. The experimental data showed that the soluble sugar content in the leaves decreased linearly in the first 4 days, the rate of decrease slowed down from the 5th to the 6th day, and entered a plateau period after the 6th day where the rate of change was close to zero. Therefore, in order to achieve sufficient energy consumption in this variety without causing excessive stress, the baseline duration of the controlled metabolic consumption treatment was determined to be 6 days.

[0051] The second part of the procedure aims to calibrate the coefficient of determination threshold used to determine population homogeneity. The procedure involves first artificially constructing three test populations with known dispersion levels. Population A consists of 50 plants at completely identical growth stages; Population B consists of a mixture of 35 identical plants and 15 plants with slight growth differences; and Population C consists of a mixture of 25 identical plants and 25 plants with significant growth differences. Diagnostic dark environment pulses are then independently applied to each of these three test populations, and their dispersion levels are calculated. coefficient of determination of concentration change curve Measurement results show that population A The value remained stable above 0.98 for group B. The values ​​are in the range of 0.94 to 0.96, while those of population C are... The value is below 0.90; considering that the dispersion of group B is within the allowable range in production, while the dispersion of group C requires intervention, the admission threshold used to distinguish whether a group needs to enter the low-light preprocessing is... It was set to 0.95. Through the two independent calibration processes described above, a set of control parameter matrices containing clear process parameters and quantitative judgment criteria was established for this new variety. This matrix was then used to guide all subsequent commercial production batches of this variety, thereby transforming a general technical method into a standardized operating procedure that can be precisely and repeatedly executed for a specific cultivation object.

[0052] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0053] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for cultivating Phalaenopsis orchids, characterized in that, This includes, firstly, performing a population health status verification step after the Phalaenopsis orchid plant's vegetative growth period ends and before the low-temperature induction treatment. This verification step obtains data on the plant population's health status within a closed environment resulting from metabolic activity during a diagnostic dark environment pulse. The concentration change characteristics are then analyzed; it is then determined whether the change characteristics meet the admission criteria defined by historical data from a healthy population; only when the change characteristics meet the admission criteria are subsequent metabolic reset and root synchronization treatments performed, wherein the metabolic reset and root synchronization include: Step a, perform a controlled metabolic depletion treatment, which includes placing the plant population under light conditions below its light compensation point for 5 to 7 consecutive days and completely stopping fertilization. Step b, during the duration of the controlled metabolic consumption treatment, at least one brief osmotic stress pulse is synergistically applied to the cultivation substrate of the plant population to perform root activity synchronization treatment. Step c: Within 24 hours after the controlled metabolic consumption treatment, a synchronized energy accumulation treatment is performed, which includes providing saturated light conditions and high phosphorus and potassium fertilizer for 2 to 3 days; after the synchronized energy accumulation treatment is completed, the plant population is subjected to a low temperature induction treatment.

2. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The population health status verification step, before judging the change characteristics, further includes: determining a dynamic health benchmark for comparison with the change characteristics based on one or more current environmental parameters and growth stage parameters of the plant population; wherein, the one or more current environmental parameters include temperature, and the growth stage parameters include the number of cultivation days.

3. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The characteristics of concentration changes, through the analysis of The coefficient of determination is calculated by linear regression fitting of the concentration-time curve. Characterization is performed; admission criteria are: ,in, This is a threshold determined by analyzing historical data of a healthy population and used to characterize the homogeneity of the population.

4. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, When the change feature does not meet the admission criteria, but the change feature corresponds to When the average rate of change of concentration meets the activity conditions calibrated by historical data of healthy population, the plant population is subjected to a period of low light treatment before performing metabolic reset and root synchronization treatment. After the low light treatment is completed, the population health status verification step is performed again.

5. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The illumination conditions in step a below its light compensation point specifically refer to a photosynthetically active radiation intensity of 5 to 15. .

6. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The high phosphorus and potassium fertilizer provided in step c has a nitrogen, phosphorus and potassium element mass ratio of 1:2:1.5 to 1:3:

2.

7. The method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The osmotic stress pulse applied in step b is achieved by applying a solution containing neutral salts through the irrigation system.

8. A method for cultivating Phalaenopsis orchids according to claim 7, characterized in that, The neutral salt is potassium sulfate, with a concentration of 50 to 150 mg / L in the solution, and each application lasts for 30 to 60 minutes.

9. A method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, The application of diagnostic dark environment pulses involves subjecting the plant population to complete darkness for 1 to 2 hours after daylight exposure ends.

10. A method for cultivating Phalaenopsis orchids according to claim 1, characterized in that, Low-temperature induction treatment involves lowering the temperature of the environment in which the plant population is located to 16 to 20 degrees Celsius.

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