Collection card for direct amplification test of non-transfer oral exfoliated cells

Abstract

The invention discloses a non-transfer acquisition card for direct amplification test of oral exfoliated cells, and particularly relates to the technical field of acquisition cards, the acquisition card comprises a support plate, the support plate is provided with an acquisition assembly, the acquisition assembly comprises a supporting plate arranged at the top of the support plate, the top of the supporting plate is provided with a substrate frame, and the substrate frame is provided with a plurality of through holes; and an acquisition layer is arranged in the middle of the substrate frame. According to the invention, the polyacrylate super absorbent layer is arranged in the middle of the collection layer, the strong water absorption and water retention characteristics of the polyacrylate super absorbent layer are utilized, oral exfoliated cells can be quickly adsorbed, the integrity of the cells can be maintained, the substrate frame is clamped with the supporting plate through the insertion plate, and the calibration blocks at four corners are matched, so that the accurate calibration of the installation position of the collection layer is realized; the information conduction piece is arranged in the sliding strip, so that the identity, the time and the geographic position of a sampler can be automatically bound, the problems that a traditional paper label is prone to being stained and damaged and information is disjointed are solved, it is ensured that samples are traceable in the whole process, and convenience is brought to a medical material evidence chain.

Description

Technical Field

[0001] The present invention relates to the technical field of collection cards, and more particularly to a collection card for direct amplification testing of non-transferable oral exfoliated cells. Background Art

[0002] Oral cells (usually referring to exfoliated oral mucosal epithelial cells) are naturally exfoliated epithelial cells from the lining of the human oral cavity (buccal mucosa, sublingual area, gums, etc.) that contain intact nuclei. These cells originate from the stratified squamous epithelium lining the oral mucosa and are constantly shed and replaced with metabolism. They are rich in intact genomic DNA (contained within the cell nucleus), making them an ideal non-invasive source of human genetic material.

[0003] DNA is the carrier of genetic information: everyone's DNA sequence (except identical twins) is unique and contains crucial genetic information that determines individual characteristics, health status, kinship, etc. DNA testing of oral exfoliated cells is a non-invasive, painless, non-traumatic method that is simple and easy to operate. Traditional oral exfoliated cell collection usually consists of two parts: an oral cell collection card and an oral collection swab. The oral cell collection card is mostly designed based on the FTA card format of Whatman Company, which adopts the form of gluing sampling paper and recording card together. The oral collection swab is composed of a round sponge stick and a long handle. The oral swab is used to collect deep into the oral cavity. After collecting oral cells, the oral swab is pressed on the oral cell collection card (FTA). This is cumbersome and difficult to track or record the collection information throughout the collection process, which increases the workload of personnel and physical evidence collection. Therefore, a non-transferable oral exfoliated cell direct amplification test collection card is provided. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, the present invention provides a collection card for direct amplification testing of non-transferable oral exfoliated cells, aiming to solve the problems raised in the above-mentioned background technology.

[0005] The present invention provides the following technical solutions: a collection card for direct amplification testing of non-transferable oral exfoliated cells, comprising a support plate, on which a collection component is provided; The collection component includes a support plate arranged on the top of the support plate, a substrate frame is arranged on the top of the support plate, a collection layer is arranged in the middle of the substrate frame, a high water absorption layer is arranged in the middle of the collection layer, and the high water absorption layer is made of polyacrylate material; The bottom of the support plate is embedded with a sliding strip, an information transmission element for traceability tracking is arranged in the sliding strip, the top of the supporting plate is fixedly provided with two stop blocks, the vertical section shape of the two stop blocks is arc-shaped, and the two stop blocks are arranged side by side, two clamping grooves are arranged through the collecting layer, and each stop block extends to the top of the collecting layer through the corresponding clamping groove.

[0006] Optionally, in the possible implementation, the inner wall of the substrate frame is provided with a calibration block at each of the four corners, and each calibration block abuts the outer side of the collecting layer. One end of the substrate frame is fixedly provided with a plug-in plate, the plug-in plate is vertically arranged, and the bottom end of the plug-in plate is plugged into the supporting plate and is clamped with the supporting plate. One end of the supporting plate and the supporting plate is provided with a mounting groove, and the two mounting grooves are communicated. The sliding strip is embedded in the mounting groove. Optionally, in the possible implementation, one side of the sliding strip is provided with a baffle, and the baffle is provided with an information recorder. The baffle and the information recorder are embedded in the bottom of the supporting plate. The inner wall of the substrate frame is provided with a dislocation slot at the bottom. The technical effects and advantages of the present application are as follows: 1. The polyacrylate superabsorbent layer arranged in the middle of the collecting layer can quickly absorb the oral cavity cells and maintain the integrity of the cells by using its strong water absorption and water retention properties, compared with the traditional FTA card, the DNA acquisition amount can be significantly improved. The chemical properties of the material can also directly rupture the cells and combine the DNA molecules, omitting the additional cell lysis step and shortening the test process. 2. The arc-shaped stop block on the top of the supporting plate penetrates the clamping groove of the collecting layer, forming a mechanical limiting structure, which not only ensures the stable installation of the collecting layer in the substrate frame, but also supports quick plugging and replacement, improves the sampling efficiency, and the substrate frame is clamped with the supporting plate through the plug-in plate, and the calibration blocks at the four corners are matched to realize accurate calibration of the installation position of the collecting layer, avoiding uneven sampling caused by installation deviation. 3. The information transmission element, such as an RFID chip or a magnetic stripe, is arranged in the sliding strip embedded in the bottom of the supporting plate, and is matched with the information recorder on the baffle to automatically bind the identity, time and geographical location of the sampler, solve the problem of easy pollution and information disconnection of traditional paper labels, ensure the traceability of the whole process of the sample, and facilitate the medical evidence chain. In summary, the polyacrylate super absorbent layer set in the middle of the collection layer can quickly absorb oral exfoliated cells and maintain cell integrity by utilizing its strong water absorption and water retention properties. The base plate frame is connected to the support plate through the plug-in plate, and cooperates with the calibration blocks at the four corners to achieve precise calibration of the installation position of the collection layer to avoid uneven sampling due to installation deviation. The information transmission parts set in the slider are used to automatically bind the identity, time and geographic location of the sampler, solve the problems of easy contamination and information disconnection of traditional paper labels, and ensure the traceability of the entire sample process to facilitate the medical evidence chain. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] To more clearly illustrate the technical solutions of the present disclosure, the following briefly introduces the drawings required for use in some embodiments. Obviously, the drawings described below are only drawings of some embodiments of the present disclosure, and those skilled in the art can also derive other drawings based on these drawings. Furthermore, the drawings described below are schematic diagrams and are not intended to limit the actual dimensions of the products, actual processes of the methods, actual timing of signals, and the like involved in the embodiments of the present disclosure.

[0008] Figure 1 It is the main view of the overall structure of the present invention.

[0009] Figure 2 It is a top view of the overall structure of the present invention.

[0010] Figure 3 Schematic diagram of the acquisition layer, substrate frame, calibration block and plug-in board of the present invention.

[0011] Figure 4 Schematic diagram of the support plate, support plate, slide bar and stop block of the present invention.

[0012] Figure 5 This is a schematic diagram of the sliding bar, information transmission component, baffle and information recorder of the present invention installed together.

[0013] Figure 6 Schematic diagram of the support plate, stopper and support plate of the present invention.

[0014] The figures are marked as follows: 1. Support plate; 2. Support plate; 3. Base plate frame; 4. Collection layer; 5. Card slot; 6. Offset notch; 7. Stop block; 8. Calibration block; 9. Plug-in board; 10. Mounting slot; 11. Slide bar; 12. Information transmission part; 13. Baffle; 14. Information recorder. DETAILED DESCRIPTION

[0015] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0016] As attached Figure 1 -Attached Figure 6 The illustrated collection card for a non-transferable oral exfoliated cell direct amplification test utilizes a collection assembly disposed on a support plate 1 and a polyacrylate superabsorbent layer disposed in the middle of a collection layer 4. Leveraging its strong water absorption and water retention properties, the card rapidly absorbs exfoliated oral cells and maintains their integrity. The baseplate frame 3 is connected to the support plate 2 via a plug-in plate 9, and calibration blocks 8 at the four corners enable precise calibration of the installation position of the collection layer 4, preventing uneven sampling due to installation deviations. The specific structural configuration of the assembly is as follows: The collection component includes a support plate 2 arranged on the top of the support plate 1, a substrate frame 3 is arranged on the top of the support plate 2, a collection layer 4 is arranged in the middle of the substrate frame 3, and a high water absorption layer is arranged in the middle of the collection layer 4. The high water absorption layer is made of polyacrylate material; As attached Figure 1 、 2 As shown in Figures 3, 4 and 6, the support plate 1 is used as a carrier, the support plate 2 is fixed on the support plate 1, and the setting of the collection layer 4 and the water absorption layer can combine the chemical water absorption and water retention properties of the high-resolution material with the collection card, which can still break the cells on the surface of the collection card and combine with DNA molecules, and can also use the strong water absorption of the functional polymer material to better absorb the collected oral exfoliated cells, obtain more DNA, and help subsequent analysis experiments.

[0017] A slide bar 11 is embedded in the bottom of the support plate 1, and an information transmission element 12 for tracing is provided in the slide bar 11; As attached Figure 1 、 2 , 4 and 5, the information transmission member 12 is used by a dedicated device to complete the information reading operation process through a magnetic card or chip card, so as to record the collected information; Two stoppers 7 are fixedly provided on the top of the support plate 2. The vertical cross-sections of the two stoppers 7 are both set to be arc-shaped, and the two stoppers 7 are arranged side by side. As attached Figure 6 As shown, the stopper 7 is provided to position the collection layer 4 so that the stopper 7 is fixed on the substrate frame 3 to ensure the stability of cell collection; Two slots 5 are formed on the collection layer 4, and each stopper 7 passes through the corresponding slot 5 and extends to the top of the collection layer 4; As attached Figure 1 、 2 As shown in , 3 and 4 , the stopper 7 passes through the card slot 5 and extends to the top of the collection layer 4, so as to limit the collection layer 4 in the substrate frame 3, facilitate the rapid replacement of the collection layer 4, and improve the convenience of disassembly and assembly of the collection layer 4; Calibration blocks 8 are provided at the four corners of the inner wall of the substrate frame 3, and each calibration block 8 abuts against the outer side of the collection layer 4; As attached Figure 3 As shown, the calibration block 8 abuts against the outside of the collection layer 4 to achieve the function of calibrating the collection layer 4 installed in the substrate frame 3, thereby ensuring the accuracy of the collection layer 4 installed on the substrate frame 3; A plug-in board 9 is fixedly provided at one end of the substrate frame 3. The plug-in board 9 is vertically arranged, and the bottom end of the plug-in board 9 is plugged into the support plate 2 and is clamped with the support plate 2. As attached Figure 1 and 3 As shown, the arrangement of the plug-in board 9 facilitates the installation of the substrate frame 3 on the support plate 2 to position the support plate 2; One end of the support plate 1 and the supporting plate 2 is provided with a mounting groove 10, and the two mounting grooves 10 are connected, and the slide bar 11 is embedded in the mounting groove 10; As attached Figure 4 and 6 As shown, the installation slot 10 is used to position the slide bar 11 to ensure the stability of the installation of the slide bar 11. The installation slot 10 can also be disassembled to select whether to use the slide bar 11 and the information transmission member 12; A baffle 13 is provided on one side of the slide bar 11, and an information recorder 14 is provided on the baffle 13. Both the baffle 13 and the information recorder 14 are embedded in the bottom of the support plate 1; As attached Figure 5 As shown, the information recorder 14 is provided to record the information collected on the collection layer 4, and to facilitate the information transmission element 12 to complete the information reading operation process through the magnetic card or chip card; The bottom of the inner wall of the substrate frame 3 is penetrated by a dislocation slot 6, and one end of the collection layer 4 is fixedly provided with 15; As attached Figure 3 As shown, the setting of the offset slot 6 is used to offset the interference between the stop block 7 and the substrate frame 3. At the same time, the setting of 15 makes it convenient for the user to hold 15 and insert the collection layer 4 into the substrate frame 3 in an inclined manner and enable the stop block 7 to pass through the card slot 5, so as to facilitate the installation of the collection layer 4 in the substrate frame 3.

[0018] The specific working principle is as follows: in the sampling preparation stage, the substrate frame 3 is vertically inserted into the clamping structure of the pallet 2 through the plug-in board 9. At this time, the calibration block 8 contacts the outside of the collection layer 4 to complete the positioning and installation of the substrate frame 3 and the pallet 2. If the traceability function needs to be enabled, the slide bar 11 is embedded in the installation groove 10 connecting the support plate 1 and the pallet 2 to ensure that the information transmission part 12 is docked with the external reading device.

[0019] When collecting oral cells, the collection layer 4 is removed and rubbed against the oral mucosa, such as the cheek or sublingual area, or the collection layer 4 is mounted on the base frame 3. The cotton swab is then rubbed against the collection layer 4. The polyacrylate-based highly absorbent layer rapidly absorbs cell fluid and exfoliated cells, directly immobilizing DNA molecules using its material properties. A stopper 7 penetrates the card slot 5 and extends to the top of the collection layer 4, limiting displacement of the collection layer 4 during the friction process and ensuring stability of the sampling area.

[0020] After sampling is complete, the collection layer 4 is mounted on the base frame 3. Sample-related information, such as the sample number and collection time, is recorded via the information recorder 14. The information transmission element 12 stores and encrypts the data. To replace the collection layer 4, the base frame 3 is tilted and removed, with the offset notches 6 clear of the interference of the stopper 7. The old collection layer is removed, the new one inserted, and then reattached to the base frame 3. The collection layer 4 can be directly used for DNA amplification testing. The DNA molecules in the highly absorbent layer can enter the testing process without the need for additional extraction steps. Later, specialized equipment can read the information transmission element 12 within the slider 11, allowing the entire sample collection process to be traced, ensuring the integrity of the chain of evidence.

[0021] Furthermore, nucleic acid protectants such as guanidine salts and EDTA are added to the collection layer 4 to inhibit DNA enzyme degradation and improve the preservation time of the sample. Antibacterial coatings such as silver ions and chitosan are coated on the surface of the collection layer 4 to prevent microbial growth from damaging DNA. This is particularly suitable for forensic or clinical long-term preservation scenarios. A pH indicator strip is set at the edge of the collection layer 4. After sampling, the color change shows whether the sample is valid, such as abnormal saliva pH, to reduce the invalid sample rate.

[0022] Moreover, metal contacts can be integrated on the outside of the information transmission component 12, which automatically transmits sample information when docked with external equipment to trigger the detection process. It can also read sample number, collection time and other data wirelessly. It is compatible with the laboratory information management system and utilizes the storage function of the information transmission component 12 to encrypt and upload the entire process data of sample collection, transportation, and testing to the chain, ensuring that the physical evidence chain cannot be tampered with, which is suitable for judicial evidence scenarios.

[0023] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A collection card for direct amplification testing of non-transferable oral exfoliated cells, comprising a support plate (1), characterized in that: A collection component is provided on the support plate (1); The collection component comprises a support plate (2) arranged on the top of the support plate (1); a substrate frame (3) is arranged on the top of the support plate (2); a collection layer (4) is arranged in the middle of the substrate frame (3); a high water absorption layer is arranged in the middle of the collection layer (4); the high water absorption layer is made of polyacrylate material; A slide bar (11) is embedded in the bottom of the support plate (1), and an information transmission component (12) for tracing the source is provided in the slide bar (11).

2. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: Two stoppers (7) are fixedly provided on the top of the support plate (2), the vertical cross-sections of the two stoppers (7) are both arranged in an arc shape, and the two stoppers (7) are arranged side by side.

3. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 2, characterized in that: Two slots (5) are formed through the collection layer (4), and each of the stoppers (7) passes through the corresponding slot (5) and extends to the top of the collection layer (4).

4. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: Calibration blocks (8) are provided at the four corners of the inner wall of the substrate frame (3), and each calibration block (8) abuts against the outer side of the collection layer (4).

5. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: A plug-in board (9) is fixedly provided at one end of the substrate frame (3), the plug-in board (9) is vertically arranged, and the bottom end of the plug-in board (9) is plugged into the support board (2) and is clamped with the support board (2).

6. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: One end of each of the support plate (1) and the supporting plate (2) is provided with a mounting groove (10), and the two mounting grooves (10) are connected, and the slide bar (11) is embedded in the mounting groove (10).

7. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: A baffle (13) is provided on one side of the slide bar (11), an information recorder (14) is provided on the baffle (13), and both the baffle (13) and the information recorder (14) are embedded in the bottom of the support plate (1).

8. The acquisition card for non-transferable oral exfoliated cell direct amplification test according to claim 1, characterized in that: The bottom of the inner wall of the substrate frame (3) is provided with a dislocation slot (6) extending through it, and one end of the collection layer (4) is fixedly provided with (15).

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