Composite peptide composition with bone and cartilage growth promoting ability as well as preparation method, application and product of composite peptide composition

Abstract

The invention belongs to the technical field of nutritional health-care food and biological medicine, and particularly relates to a composite peptide composition with bone and cartilage growth promoting ability and a preparation method, application and a product of the composite peptide composition. The composite peptide composition provided by the invention consists of marine fishbone collagen peptide, chondropeptide and eggshell membrane peptide. The composite peptide composition and the composite peptide preparation are good in safety, and the survival rate of chondrocytes and osteoblasts treated by hydrogen peroxide can be remarkably increased. Gene expressions of ACAN, COL1 alpha1, COL2 alpha1, CHST3, HAS3 and OCN in chondrocytes and osteoblasts are up-regulated, and it is prompted that the compound has protection and promotion effects on bone-related cell functions. The composite peptide composition and the composite peptide preparation synchronously act on bone longitudinal growth and cartilage health through multi-target design, provide comprehensive nutrition support for bone and cartilage synergistic development, and have the characteristics of high safety, comprehensive functions and good palatability.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of nutraceuticals and biomedicine, and specifically relates to a composite peptide composition with bone and cartilage growth-promoting ability, a preparation method, application and product thereof. BACKGROUND

[0002] The growth and development of children are affected by multiple factors such as genetics, nutrition, hormone levels, and living environment. Currently, children are facing the problem of growth retardation. In clinical practice, endocrine diseases such as growth hormone deficiency are the main factors leading to pathological short stature, but more children show non-specific growth retardation, which is often closely related to nutritional imbalance, chronic diseases or insufficient environmental stimulation.

[0003] In traditional treatment methods, subcutaneous injection of recombinant human growth hormone can effectively improve the linear growth of specific children, but the invasive administration method can easily lead to a decrease in treatment compliance, and long-term use may cause potential risks such as metabolic disorders. More notably, this therapy is only suitable for patients with diagnosed growth hormone deficiency, and such cases account for a limited proportion of short children. In the face of a large population of subclinical growth retardation, the development of non-invasive intervention programs has become an important research direction in the field of pediatrics.

[0004] Oral growth-promoting products show unique value in this context. Such preparations can intervene through multiple pathways such as nutritional fortification and metabolic regulation, breaking through the limitations of injection therapy and providing solutions for the widespread nutrition-related growth retardation. The design of their dosage forms significantly improves the convenience of medication, especially for children who need long-term intervention. The development of oral growth-promoting products has multiple practical significance: from the perspective of public health, it can establish a growth retardation prevention and control system covering a wider population; from the clinical treatment level, it provides an alternative for children who cannot receive hormone therapy; from the social and economic dimension, the popularization of over-the-counter nutritional supplements can effectively reduce the consumption of medical resources.

[0005] The oral growth-promoting products in the prior art can be divided into three categories according to the mechanism of action: nutritional supplement type preparations, hormone regulation type preparations and traditional Chinese medicine compound preparations, and each category has significant differences in composition and mode of action. For example, a composition containing calcium, magnesium, zinc and vitamin D3 is provided in a patent, and the amount of calcium taken to improve bone health, prevent and relieve osteoporosis is only one-third of the daily dose for traditional osteoporosis treatment and prevention. Patent CN119235018A provides a nutritional powder for promoting the growth of children's bones and a preparation method, and the nutritional powder comprises trehalose, strawberry fruit powder, L-calcium lactate, malt dextrin, gamma-aminobutyric acid, L-lysine, taurine, bone collagen protein, zinc gluconate, fructooligosaccharide, yam powder, poria cocos powder, stevioside, VD3 dry powder and VA acetic acid ester powder.

[0006] The nutritional supplement type product takes calcium, zinc, vitamin D and other trace elements as the core components, and promotes bone development by strengthening the supply of key nutrients for bone metabolism. This type of product usually uses organic salts such as calcium gluconate and zinc lactate to improve absorption, and is also combined with vitamin K2 to enhance the directional deposition of calcium. However, it should be noted that the bioavailability of mineral elements in different preparations varies, and excessive supplementation may cause intestinal irritation or metabolic burden. The hormone regulation type product mainly contains amino acid precursors such as L-arginine and gamma-aminobutyric acid, and plays a role by stimulating the growth hormone secretion axis. Some high-end products may contain bone collagen peptide components, but all are type I collagen and are added as a concept.

[0007] The traditional Chinese medicine compound preparation mainly uses traditional tonifying medicinal materials, and the typical formula contains extracts of medicinal materials such as radix astragali, oyster, and yam, emphasizing the overall action concept of "spleen-kidney regulation". However, the palatability of traditional Chinese medicine compound is poor, the compliance of children is low, and it is difficult to take for a long time.

[0008] In view of the above situation, the present application finds that the existing products have the following defects: (1) only for hard bone mineralization, ignoring the need for cartilage development, and lacking key substances such as polysaccharides and sialic acid for assisting growth and development; (2) only using bone collagen peptide as a concept, resulting in low absorption and utilization rate of supplemented calcium, zinc and other ions, and the calcium and zinc content is not naturally present in the product; (3) poor palatability: low compliance of children, difficult to take for a long time. Based on this, there is an urgent need to develop a composite peptide composition with bone and cartilage growth-promoting ability. SUMMARY

[0009] In view of the above shortcomings, the present application provides a composite peptide composition with bone and cartilage growth-promoting ability, as well as a preparation method, application and product thereof.

[0010] The technical scheme of the present application is as follows: In one aspect, the present application provides a composite peptide composition with both bone and cartilage growth-promoting ability, which is composed of marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides.

[0011] Specifically, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 10-40:5-20:5-15.

[0012] Preferably, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 15-35:8-15:8-12.

[0013] Further preferably, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 25-35:10-15:10-12.

[0014] Still further preferably, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 25-26, 26-27, 27-28, 28-29, 29-30, 30-31, 31-32, 32-33, 33-34 or 34-35:10-11, 11-12, 12-13, 13-14 or 14-15:10-11 or 11-12.

[0015] Still further preferably, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 25:10:12.

[0016] Still further preferably, the weight ratio of the marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides is 35:15:12.

[0017] In another aspect, the present application provides a preparation method of the composite peptide composition as described in any one of the above, which comprises mixing marine fish bone collagen peptides, cartilage peptides and eggshell membrane peptides according to a weight ratio.

[0018] In another aspect, the present application provides a composite peptide preparation with both bone and cartilage growth-promoting ability, which comprises the composite peptide composition as described in any one of the above.

[0019] Specifically, the composite peptide preparation further comprises a medicinal and edible peptide ingredient.

[0020] Preferably, the medicinal and edible peptide ingredient comprises any one or more of bovine colostrum peptides, oyster peptides, bird's nest peptides, chicken gizzard peptides, yam peptides and mulberry peptides.

[0021] Further preferably, the composite peptide preparation comprises composite peptide preparation 1, composite peptide preparation 2, composite peptide preparation 3 or composite peptide preparation 4. The composite peptide preparation 1 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide; The composite peptide preparation 2 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken's gizzard lining peptide; The composite peptide preparation 3 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide and yam peptide; The composite peptide preparation 4 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide, yam peptide and mulberry peptide.

[0022] Further preferably, the weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide in the composite peptide preparation 1 is 15-35:8-15:8-12:5-20:5-20:5-10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken's gizzard lining peptide in the composite peptide preparation 2 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide and yam peptide in the composite peptide preparation 3 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10:5-20; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide, yam peptide and mulberry peptide in the composite peptide preparation 4 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10:5-20:5-20.

[0023] Further preferably, the weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide in the composite peptide preparation 1 is 35:10:8:15:15:10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken's gizzard lining peptide in the composite peptide preparation 2 is 15:10:10:15:10:10:5; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide and yam peptide in the composite peptide preparation 3 is 30:8:10:15:10:10:8:15; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide, yam peptide and mulberry peptide in the composite peptide preparation 4 is 30:10:8:10:15:10:5:15:20.

[0024] In another aspect, the present application provides a preparation method of the composite peptide preparation according to any one of the above, which comprises mixing the composite peptide composition and the comestible peptide ingredient according to the weight ratio.

[0025] In another aspect, the present application provides a food product, characterized in that the food product comprises the composite peptide composition or the composite peptide preparation according to any one of the above.

[0026] Preferably, the food product comprises any one or more of candies, soy milk, yogurt, milk, fruit juice, canned food, biscuits, chocolate, cakes, butter, cheese, cream, milk powder, formula milk powder, ice cream, fruit jam, fruit puree, preserved fruit, bread, egg roll, protein beverage, solid beverage, lactic acid bacteria beverage, carbonated beverage, coffee, jelly, protein bar, energy bar, energy gel, and puffed food.

[0027] Preferably, the food product comprises human food or pet food.

[0028] In another aspect, the present application provides a health product, characterized in that the health product comprises the composite peptide composition or the composite peptide preparation according to any one of the above.

[0029] Specifically, the health product further comprises a nutritionally acceptable nutrient.

[0030] Preferably, the nutrient comprises one or more of dietary fiber, protein, lipid substance, mineral, and vitamin.

[0031] Specifically, the dosage form of the health product comprises any one or more of solid dosage form, semi-solid dosage form, and liquid dosage form.

[0032] Preferably, the dosage form of the health product comprises tablet, capsule, soft capsule, granule, pill, gummy candy, powder, oral liquid, or drop.

[0033] In another aspect, the present application provides use of the composite peptide composition or the composite peptide preparation according to any one of the above in the preparation of a drug for promoting the growth of bone and cartilage.

[0034] In another aspect, the present application provides a drug product, characterized in that the drug product comprises the composite peptide composition or the composite peptide preparation according to any one of the above.

[0035] Specifically, the drug product further comprises one or more pharmaceutically acceptable excipients.

[0036] Preferably, the pharmaceutically acceptable adjuvant includes, but is not limited to, solvents, solubilizers, co-solvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesion agents, integrating agents, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, thickening agents, inclusion agents, humectants, absorbents, diluents, flocculants and deflocculants, filtration aids, release retardants, high molecular backbone materials or film-forming materials.

[0037] Specifically, the dosage form of the pharmaceutical product includes a gastrointestinal administration dosage form or a non-gastrointestinal administration dosage form.

[0038] Preferably, the gastrointestinal administration dosage form includes, but is not limited to, tablets, powders, granules, solutions, capsules, emulsions, suspensions, and oils.

[0039] Preferably, the gastrointestinal administration dosage form includes, but is not limited to, injection administration dosage forms, respiratory administration dosage forms, skin administration dosage forms, mucosal administration dosage forms, and cavity administration dosage forms.

[0040] In another aspect, the present application provides a method for promoting bone and cartilage growth, the method comprising administering to a subject an effective amount of a composite peptide composition, a composite peptide preparation, a food, a health product or a pharmaceutical product.

[0041] Specifically, the subject is a mammal.

[0042] Preferably, the subject is a human.

[0043] The beneficial effects of the present application are: 1. The composite peptide composition of the present application comprises a core bone promoting component, cartilage peptide, marine fish bone collagen peptide and eggshell membrane peptide. Among them, the cartilage peptide contains type II collagen peptide and chondroitin sulfate, which supports chondrocyte proliferation and joint health; the marine fish bone collagen peptide provides type I collagen peptide and calcium and phosphorus, which directly promotes hard bone mineralization; the eggshell membrane peptide is rich in hyaluronic acid and type X collagen peptide, which enhances the toughness of the periosteum.

[0044] 2. The composite peptide preparation of the present application adds a growth synergistic auxiliary component on the basis of the composite peptide composition: Bovine colostrum: provides lactoferrin and immunoglobulin, promotes iron absorption and immunity; Curd peptide: contains sialic acid (neuraminic acid), promotes neural development and cell membrane synthesis; Mulberry peptide: contains anthocyanins and growth-promoting polyphenols, improves taste and stimulates growth hormone secretion, and improves product palatability; Oyster peptide: provides taurine, zinc, and lysine to promote the activation of the growth axis (GH-IGF-1); Yam peptide: contains mucus polysaccharide, strengthens the spleen and promotes digestion, and improves the palatability of the product; Chicken Gizzard Peptide: Regulates gastrointestinal function and enhances nutrient absorption.

[0045] 3. The composite peptide composition and composite peptide preparation of the present invention have good safety and can significantly improve the survival rate of chondrocytes and osteoblasts after hydrogen peroxide treatment. ACAN, COL1a1, COL2a1, CHST3, HAS3, OCN Gene expression suggests that it has protective and promoting effects on bone-related cell function. The composite peptide composition and composite peptide preparation of the present invention, through multi-target design, simultaneously acts on longitudinal bone growth and cartilage health, providing comprehensive nutritional support for the coordinated development of bone and cartilage, with high safety, comprehensive functions, and good palatability. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 The figures are the results of toxicity test of the composite peptide composition and composite peptide preparation on chondrocytes.

[0047] Figure 2 The figures are the results of toxicity test of the composite peptide composition and composite peptide preparation on osteoblasts.

[0048] Figure 3 The results of the toxicity test of the composite peptide composition and composite peptide preparation on H2O2-induced chondrocytes; "***" in the figure represents p < 0.001 compared with the control group; "###" represents p < 0.001 compared with the model group.

[0049] Figure 4 The results of the toxicity test of the composite peptide composition and composite peptide preparation on H2O2-induced osteoblasts; "***" in the figure represents p < 0.001 compared with the control group; "###" represents p < 0.001 compared with the model group.

[0050] Figure 5 Effects of complex peptide compositions and complex peptide preparations on chondrocytes ACAN Effect of mRNA expression; "***" in the figure represents p < 0.001 compared with the control group; "###" represents p < 0.001 compared with the model group.

[0051] Figure 6 Effects of complex peptide compositions and complex peptide preparations on chondrocytes COL2a1 Effect of mRNA expression; "***" in the figure represents p < 0.001 compared with the control group; "###" represents p < 0.001 compared with the model group.

[0052] Figure 7Effects of the composite peptide composition and the composite peptide preparation on the mRNA expression in chondrocytes CHST3 *** represents p<0.001 compared with the control group; and ### represents p<0.001 compared with the model group.

[0053] Figure 8 Effects of the composite peptide composition and the composite peptide preparation on the mRNA expression in chondrocytes HAS3 *** represents p<0.001 compared with the control group; and ### represents p<0.001 compared with the model group.

[0054] Figure 9 Effects of the composite peptide composition and the composite peptide preparation on the mRNA expression in osteoblasts ACAN *** represents p<0.001 compared with the control group; and ### represents p<0.001 compared with the model group.

[0055] Figure 10 Effects of the composite peptide composition and the composite peptide preparation on the mRNA expression in osteoblasts COL1a1 *** represents p<0.001 compared with the control group; and ### represents p<0.001 compared with the model group.

[0056] Figure 11 Effects of the composite peptide composition and the composite peptide preparation on the mRNA expression in osteoblasts OCN *** represents p<0.001 compared with the control group; and ### represents p<0.001 compared with the model group. DETAILED DESCRIPTION

[0057] The application will be further clarified by the following examples, which are not intended to limit the application. The experimental methods used in the following examples are conventional unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available unless otherwise specified.

[0058] The Saos-2 human osteoblasts, SW 1353 human chondrocytes and Saos-2 cell special culture medium of the application are purchased from Wuhan Punsai Life Science and Technology Co., Ltd., and the item numbers are CL-0202, CL-0447B and CM-0202, respectively; the FBS is purchased from VicaCell, and the item number is C04001; the DMEM culture medium is purchased from Sigma, and the item number is D7777; the penicillin-streptomycin solution (100X) is purchased from Biyun Tian, and the item number is C0222; and the trypsin 0.25% (containing EDTA and phenol red) is purchased from Gibco, and the item number is 25200056.

[0059] The cartilage peptides used in the present application are chicken breast cartilage-derived cartilage peptides (20240523-2), marine fish bone collagen peptides (20240422-1), eggshell membrane peptides (20240214-2), bovine colostrum peptides (20240525-1), oyster peptides (20240311-1), bird's nest peptides (20240324-3), chicken gizzard peptides (20240512-1), yam peptides (20240513-1), mulberry peptides (20240425-1), which are provided by Beijing Shengmei Biological Technology Co., Ltd. The bovine bone collagen peptides, cartilage extracts, and eggshell membrane extracts used in the present application are provided by Beijing Shengmei Biological Technology Co., Ltd.

[0060] Example 1 The composite peptide preparation of Example 1 is a composition of marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, and bird's nest peptides. The weight ratio of the marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, and bird's nest peptides is 35:10:8:15:15:10.

[0061] The preparation method of the composite peptide preparation of Example 1 is to mix marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, and bird's nest peptides according to the weight ratio to obtain the composite peptide preparation.

[0062] Example 2 The composite peptide preparation of Example 2 is a composition of marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, bird's nest peptides, and chicken gizzard peptides. The weight ratio of the marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, bird's nest peptides, and chicken gizzard peptides is 15:10:10:15:10:10:5.

[0063] The preparation method of the composite peptide preparation of Example 2 is to mix marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, bird's nest peptides, and chicken gizzard peptides according to the weight ratio to obtain the composite peptide preparation.

[0064] Example 3 The composite peptide preparation of Example 3 is a composition of marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, bird's nest peptides, chicken gizzard peptides, and yam peptides. The weight ratio of the marine fish bone collagen peptides, cartilage peptides, eggshell membrane peptides, bovine colostrum peptides, oyster peptides, bird's nest peptides, chicken gizzard peptides, and yam peptides is 30:8:10:15:10:10:8:15.

[0065] The preparation method of the composite peptide preparation of Example 3 is as follows: marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide and yam peptide are mixed according to the weight ratio to obtain the composite peptide preparation.

[0066] Example 4 The composite peptide preparation of Example 4 is a composition of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide, yam peptide and mulberry peptide. The weight ratio of the marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide, yam peptide and mulberry peptide is 30:10:8:10:15:10:5:15:20.

[0067] The preparation method of the composite peptide preparation of Example 4 is as follows: marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide, yam peptide and mulberry peptide are mixed according to the weight ratio to obtain the composite peptide preparation.

[0068] Example 5 The composite peptide composition of Example 5 is a composition of marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide. The weight ratio of the marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide is 25:10:12.

[0069] The preparation method of the composite peptide composition of Example 5 is as follows: marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide are mixed according to the weight ratio to obtain the composite peptide composition.

[0070] Comparative Example 1 The composite peptide preparation of Comparative Example 1 is a composition of bovine colostrum peptide, oyster peptide and bird's nest peptide. The weight ratio of the bovine colostrum peptide, oyster peptide and bird's nest peptide is 15:20:5.

[0071] The preparation method of the composite peptide preparation of Comparative Example 1 is as follows: bovine colostrum peptide, oyster peptide and bird's nest peptide are mixed according to the weight ratio to obtain the composite peptide preparation.

[0072] Comparative Example 2 The composite peptide preparation of Comparative Example 2 is a composition of bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken internal gold peptide. The weight ratio of the bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken internal gold peptide is 5:15:8:10.

[0073] The preparation method of the composite peptide preparation of Comparative Example 2 is as follows: bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken internal gold peptide are mixed according to the weight ratio to obtain the composite peptide preparation.

[0074] Comparative Example 3 The complex peptide preparation of Comparative Example 3 is a composition of bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, and yam peptide. The weight ratio of the bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, and yam peptide is 10:12:8:10:15.

[0075] The preparation method of the complex peptide composition of Comparative Example 3 is to mix bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, and yam peptide according to the weight ratio to obtain a complex peptide preparation.

[0076] Comparative Example 4 The complex peptide preparation of Comparative Example 4 is a composition of bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide. The weight ratio of the bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide is 15:10:10:8:15:12.

[0077] Comparative Example 5 The complex peptide preparation of Comparative Example 5 is a composition of marine fish bone collagen peptide, cartilage peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide. The weight ratio of the marine fish bone collagen peptide, cartilage peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide is 30:10:15:8:10:10:15:10.

[0078] The preparation method of the complex peptide preparation of Comparative Example 5 is to mix marine fish bone collagen peptide, cartilage peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide according to the weight ratio to obtain a complex peptide preparation.

[0079] Comparative Example 6 The complex peptide preparation of Comparative Example 6 is a composition of marine fish bone collagen peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide. The weight ratio of the marine fish bone collagen peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide is 25:8:10:10:8:5:15:20.

[0080] The preparation method of the complex peptide preparation of Comparative Example 6 is to mix marine fish bone collagen peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide according to the weight ratio to obtain a complex peptide preparation.

[0081] Comparative Example 7 The complex peptide preparation of Comparative Example 7 is a composition of cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide. The weight ratio of the cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard suet peptide, yam peptide, and mulberry peptide is 15:10:5:10:8:10:15:20.

[0082] The preparation method of the complex peptide preparation of Comparative Example 7 is as follows: the cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken's gizzard lining peptide, yam peptide, and mulberry peptide are mixed according to the weight ratio to obtain the complex peptide preparation.

[0083] Comparative Example 8 The complex peptide composition of Comparative Example 8 is a composition of marine fish bone collagen peptide, cartilage extract, and eggshell membrane peptide. The weight ratio of the marine fish bone collagen peptide, cartilage extract, and eggshell membrane peptide is 25:10:12.

[0084] The preparation method of the complex peptide composition of Comparative Example 8 is as follows: the marine fish bone collagen peptide, cartilage extract, and eggshell membrane peptide are mixed according to the weight ratio to obtain the complex peptide composition.

[0085] Comparative Example 9 The complex peptide composition of Comparative Example 9 is a composition of marine fish bone collagen peptide, cartilage extract, and eggshell membrane extract. The weight ratio of the marine fish bone collagen peptide, cartilage extract, and eggshell membrane extract is 25:10:12.

[0086] The preparation method of the complex peptide composition of Comparative Example 9 is as follows: the marine fish bone collagen peptide, cartilage extract, and eggshell membrane extract are mixed according to the weight ratio to obtain the complex peptide composition.

[0087] Comparative Example 10 The complex peptide composition of Comparative Example 10 is a composition of bovine bone collagen peptide, cartilage extract, and eggshell membrane extract. The weight ratio of the bovine bone collagen peptide, cartilage extract, and eggshell membrane extract is 25:10:12.

[0088] The preparation method of the complex peptide composition of Comparative Example 10 is as follows: the bovine bone collagen peptide, cartilage extract, and eggshell membrane extract are mixed according to the weight ratio to obtain the complex peptide composition.

[0089] Experimental Example 1 Cell Passage and Culture After the cells are cultured in the cell culture box for 3 h, the liquid is changed, and the cells are continuously cultured for 24 h. Then the culture solution is removed, 0.25% trypsin is added for digestion for 5 min, the same volume of cell culture solution is added to terminate the digestion, the cells are collected into a 10 mL centrifuge tube, centrifuged at 1200 rpm for 3 min, the supernatant is removed, and new cell culture solution is added and uniformly blown to an average of 3 culture dishes, and then the culture solution is supplemented. When the cells grow to 80% of the full plate, the above operation is repeated for passage. The culture medium of the chondrocytes contains 10% fetal bovine serum, 1% penicillin and streptomycin, and the culture medium of the osteoblasts is a special culture medium for Saos-2 cells purchased.

[0090] ​Experimental Example 2 Toxicity test of the complex peptide composition and the complex peptide preparation on chondrocytes and osteoblasts 1. Experimental method SW 1353 human chondrocytes (or Saos-2 human osteoblasts) were collected into 10 mL centrifuge tubes after the cells were spread on a plate, centrifuged at 1200 rpm for 3 min, the supernatant was removed, and new cell culture solution was added, uniformly blown, and counted using a hemocytometer. 10 cells were spread in each well of a 96-well plate, and the total volume was 200 μL, which was cultured overnight at 37°C in a 5% CO2condition, and then divided into a control group, Example 1 group-Example 5 group, Comparative Example 1 group-Comparative Example 10 group, marine fish bone collagen peptide group, cartilage peptide group, and eggshell membrane peptide group, and the cells in each group were treated as follows: 4 Control group: the same volume of culture medium was added.

[0091] Example 1 group: the complex peptide preparation of Example 1 was added after being prepared into a solution with a culture medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, and bird's nest peptide were 350 μg / mL, 100 μg / mL, 80 μg / mL, 150 μg / mL, 150 μg / mL, and 100 μg / mL, respectively.

[0092] Example 2 group: the complex peptide preparation of Example 2 was added after being prepared into a solution with a culture medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, and chicken gizzard peptide were 150 μg / mL, 100 μg / mL, 100 μg / mL, 150 μg / mL, 100 μg / mL, 100 μg / mL, and 50 μg / mL, respectively.

[0093] Example 3 group: the complex peptide preparation of Example 3 was added after being prepared into a solution with a culture medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, and yam peptide were 300 μg / mL, 80 μg / mL, 100 μg / mL, 150 μg / mL, 100 μg / mL, 100 μg / mL, 80 μg / mL, and 150 μg / mL, respectively.

[0094] Example 4 group: the complex peptide preparation of Example 4 was added after being prepared into a solution with a culture medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide, and mulberry peptide were 300 μg / mL, 100 μg / mL, 80 μg / mL, 100 μg / mL, 150 μg / mL, 100 μg / mL, 50 μg / mL, 150 μg / mL, and 200 μg / mL, respectively.

[0095] ​Example 5 group: The complex peptide composition of Example 5 was added after being formulated into a solution with the medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, and eggshell membrane peptide were 250 μg / mL, 100 μg / mL, and 120 μg / mL, respectively.

[0096] Comparative Example 1 group: The complex peptide preparation of Comparative Example 1 was added after being formulated into a solution with the medium, and the mass concentrations of bovine colostrum peptide, oyster peptide, and bird's nest peptide were 150 μg / mL, 200 μg / mL, and 50 μg / mL, respectively.

[0097] Comparative Example 2 group: The complex peptide preparation of Comparative Example 2 was added after being formulated into a solution with the medium, and the mass concentrations of bovine colostrum peptide, oyster peptide, bird's nest peptide, and chicken gizzard peptide were 50 μg / mL, 150 μg / mL, 80 μg / mL, and 100 μg / mL, respectively.

[0098] Comparative Example 3 group: The complex peptide preparation of Comparative Example 3 was added after being formulated into a solution with the medium, and the mass concentrations of bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, and yam peptide were 100 μg / mL, 120 μg / mL, 80 μg / mL, 100 μg / mL, and 150 μg / mL, respectively.

[0099] Comparative Example 4 group: The complex peptide preparation of Comparative Example 4 was added after being formulated into a solution with the medium, and the mass concentrations of bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide, and mulberry peptide were 150 μg / mL, 100 μg / mL, 100 μg / mL, 80 μg / mL, 150 μg / mL, and 120 μg / mL, respectively.

[0100] Comparative Example 5 group: The complex peptide preparation of Comparative Example 5 was added after being formulated into a solution with the medium, and the mass concentrations of marine fish bone collagen peptide, cartilage peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide, and mulberry peptide were 300 μg / mL, 100 μg / mL, 150 μg / mL, 80 μg / mL, 100 μg / mL, 100 μg / mL, 150 μg / mL, and 100 μg / mL, respectively.

[0101] Comparative Example 6 group: The complex peptide preparation of Comparative Example 6 was added after being formulated into a solution with the medium, and the mass concentrations of marine fish bone collagen peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide, and mulberry peptide were 250 μg / mL, 80 μg / mL, 100 μg / mL, 100 μg / mL, 80 μg / mL, 50 μg / mL, 150 μg / mL, and 200 μg / mL, respectively.

[0102] Comparative Example 7 Group: The complex peptide preparation of Comparative Example 7 was added after being prepared into a solution with the culture medium, and the mass concentrations of the cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken internal gold peptide, yam peptide, and mulberry peptide were 150 μg / mL, 100 μg / mL, 50 μg / mL, 100 μg / mL, 80 μg / mL, 100 μg / mL, 150 μg / mL, and 200 μg / mL, respectively.

[0103] Comparative Example 8 Group: The complex peptide composition of Comparative Example 8 was added after being prepared into a solution with the culture medium, and the mass concentrations of the marine fish bone collagen peptide, cartilage extract, and eggshell membrane peptide were 250 μg / mL, 100 μg / mL, and 120 μg / mL, respectively.

[0104] Comparative Example 9 Group: The complex peptide composition of Comparative Example 9 was added after being prepared into a solution with the culture medium, and the mass concentrations of the marine fish bone collagen peptide, cartilage extract, and eggshell membrane extract were 250 μg / mL, 100 μg / mL, and 120 μg / mL, respectively.

[0105] Comparative Example 10 Group: The complex peptide composition of Comparative Example 10 was added after being prepared into a solution with the culture medium, and the mass concentrations of the bovine bone collagen peptide, cartilage extract, and eggshell membrane extract were 250 μg / mL, 100 μg / mL, and 120 μg / mL, respectively.

[0106] Marine fish bone collagen peptide group: The marine fish bone collagen peptide culture medium was prepared into a solution, and the mass concentration of the marine fish bone collagen peptide was 250 μg / mL.

[0107] Cartilage peptide group: The cartilage peptide was prepared into a solution with the culture medium, and the mass concentration of the cartilage peptide was 100 μg / mL.

[0108] Eggshell membrane peptide group: The eggshell membrane peptide was prepared into a solution with the culture medium, and the mass concentration of the eggshell membrane peptide was 120 μg / mL.

[0109] Four replicate wells were set for each group, and after the administration was completed, the culture was performed for 24 h, 10% CCK-8-containing culture solution was added to each well, and the reaction was performed for 2 h, and the absorbance at 450 nm was measured using an enzyme marker.

[0110] 2. Experimental results The results of the toxicity determination of the complex peptide composition and the complex peptide preparation on the chondrocytes are shown in Table 1, and the results of the toxicity determination on the osteoblasts are shown in Table 2. Figure 1 Figure 2 The determination results show that the Example 1 group-Example 5 group, Comparative Example 1 group-Comparative Example 10 group, marine fish bone collagen peptide group, cartilage peptide group, and eggshell membrane peptide group have good safety for the chondrocytes and the osteoblasts, and the cell survival rates have no significant difference compared with the control group.​

[0111] Experimental Example 3 Effects of the composite peptide composition and the composite peptide preparation on the activity of chondrocytes and osteoblasts after hydrogen peroxide treatment 1. Experimental method SW 1353 human chondrocytes (or Saos-2 human osteoblasts) were collected into a 10 mL centrifuge tube after the cells were spread on a plate, centrifuged at 1200 rpm for 3 min, the supernatant was removed, and new cell culture solution was added, uniformly blown, and counted using a hemocytometer. 10 4 cells were spread in each well of a 96-well plate, and the total volume was 200 μL, and the cells were cultured at 37°C, 5% CO2 overnight, and then divided into a control group, an H2O2 group, an Example 1 group to an Example 5 group, a Comparative Example 1 group to a Comparative Example 10 group, a marine fish bone collagen peptide group, a cartilage peptide group, and an eggshell membrane peptide group, and the cells in each group were administered as follows: The control group: the same volume of culture medium was added.

[0112] The H2O2 group: the same volume of culture medium was added.

[0113] The treatment method of the Example 1 group to the Example 5 group, the Comparative Example 1 group to the Comparative Example 10 group, the marine fish bone collagen peptide group, the cartilage peptide group, and the eggshell membrane peptide group was the same as that of Experimental Example 2.

[0114] Four duplicate wells were set for each group, and after the administration was completed, the cells were cultured for 24 h; the cells in each group were treated with 600 μM hydrogen peroxide for 3 h, except for the control group; 10% CCK-8-containing culture solution was added to each well, and the reaction was performed for 2 h, and the absorbance at 450 nm was measured using an enzyme marker.

[0115] 2. Experimental results The results of the toxicity determination of the composite peptide composition and the composite peptide preparation on H2O2-induced chondrocytes are shown in Table 1, and the results of the toxicity determination of the composite peptide composition and the composite peptide preparation on H2O2-induced osteoblasts are shown in Table 2. Figure 3 Figure 4 The results show that the composite peptide composition and the composite peptide preparation provided by the present application can significantly improve the cell survival rate of chondrocytes or osteoblasts after H2O2 treatment.

[0116] Experimental Example 4 Fluorescent quantitative PCR for expression of related genes 1. Experimental method SW 1353 human chondrocytes (or Saos-2 human osteoblasts) were collected into a 10 mL centrifuge tube after the cells were spread on a plate, centrifuged at 1200 rpm for 3 min, the supernatant was removed, and new cell culture solution was added, uniformly blown, and counted using a hemocytometer. 10 5 ​The cells were cultured overnight, and then divided into a control group, an H2O2 group, Example 1 to Example 5 groups, Comparative Example 1 to Comparative Example 10 groups, a marine fish bone collagen peptide group, a cartilage peptide group, and an eggshell membrane peptide group. The cells in each group were administered in the following manner: Control group: an equal volume of culture medium was added.

[0117] H2O2 group: an equal volume of culture medium was added.

[0118] The treatment methods for the Example 1 to Example 5 groups, Comparative Example 1 to Comparative Example 10 groups, marine fish bone collagen peptide group, cartilage peptide group, and eggshell membrane peptide group were as described in Experimental Example 2.

[0119] Two duplicate wells were set for each group. After administration, the cells were cultured for 12 h. The cells in each group were treated with 400 μM hydrogen peroxide for 6 h, except for the control group. The cell RNA was extracted according to the instructions, and cDNA was synthesized by reverse transcription. RT-qPCR was performed using the primers in Table 1 and the reaction system in Table 2. The PCR program is shown in Table 3.

[0120] Table 1: Primer information

[0121] Table 2: Reaction system

[0122] Table 3: PCR program

[0123] 2, Effect of the composite peptide composition and composite peptide preparation on chondrocytes ACAN, COL2a1, CHST3, HAS3 The effect of the composite peptide composition and composite peptide preparation on chondrocytes is shown in Table 2. The results show that the composite peptide composition and composite peptide preparation provided by the present application can significantly increase the expression of ACAN, COL2a1, CHST3, HAS3 in chondrocytes after H2O2 treatment. Figures 5-8 ACAN, COL2a1, CHST3, HAS3 3, Effect of the composite peptide composition and composite peptide preparation on osteoblasts The effect of the composite peptide composition and composite peptide preparation on chondrocytes

[0124] is shown in Table 2. The results show that the composite peptide composition and composite peptide preparation provided by the present application can significantly increase the expression of ACAN, COL1a1, OCN in chondrocytes after H2O2 treatment. ACAN, COL1a1, OCN Figures 9-11 ACAN, COL1a1, OCN

[0125] ​​​​The above detailed description is a specific description of one of the possible embodiments of the present application, which is not intended to limit the patent scope of the present application. It should be noted that any equivalent implementation or change made without departing from the present application shall be included in the scope of the technical solutions of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A composite peptide composition having both bone and cartilage growth promoting capabilities, characterized in that: The composite peptide composition consists of marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide.

2. The composite peptide composition according to claim 1, characterized in that The weight ratio of the marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide is 10-40:5-20:5-15.

3. The composite peptide composition according to claim 2, characterized in that The weight ratio of the marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide is 15-35:8-15:8-12.

4. The composite peptide composition according to claim 3, characterized in that The weight ratio of the marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide is 25-35:10-15:10-12.

5. The composite peptide composition according to claim 3, characterized in that The weight ratio of the marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide is 25:10:

12.

6. The method for preparing the composite peptide composition according to any one of claims 1 to 5, characterized in that: The preparation method comprises: mixing marine fish bone collagen peptide, cartilage peptide and eggshell membrane peptide according to a weight ratio.

7. A composite peptide preparation having both bone and cartilage growth promoting capabilities, characterized in that: The complex peptide preparation comprises the complex peptide composition according to any one of claims 1 to 5.

8. The complex peptide preparation according to claim 7, characterized in that The composite peptide preparation further comprises medicine-food homologous peptide components.

9. The complex peptide preparation according to claim 8, characterized in that The medicine-food homologous peptide components include any one or more of bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide, and mulberry peptide.

10. The complex peptide preparation according to claim 9, characterized in that The composite peptide preparation includes composite peptide preparation 1, composite peptide preparation 2, composite peptide preparation 3 or composite peptide preparation 4; The composite peptide preparation 1 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide; The composite peptide preparation 2 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken gizzard peptide; The composite peptide preparation 3 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide and yam peptide; The composite peptide preparation 4 is composed of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide and mulberry peptide.

11. The complex peptide preparation according to claim 10, characterized in that The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide in the composite peptide preparation 1 is 15-35:8-15:8-12:5-20:5-20:5-10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken gizzard peptide in the composite peptide preparation 2 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide and yam peptide in the composite peptide preparation 3 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10:5-20; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide and mulberry peptide in the composite peptide preparation 4 is 15-30:8-13:8-10:5-15:5-15:5-10:5-10:5-20:5-20.

12. The complex peptide preparation according to claim 11, characterized in that The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide and bird's nest peptide in the composite peptide preparation 1 is 35:10:8:15:15:10; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide and chicken gizzard peptide in the composite peptide preparation 2 is 15:10:10:15:10:10:5; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide and yam peptide in the composite peptide preparation 3 is 30:8:10:15:10:10:8:15; The weight ratio of marine fish bone collagen peptide, cartilage peptide, eggshell membrane peptide, bovine colostrum peptide, oyster peptide, bird's nest peptide, chicken gizzard peptide, yam peptide and mulberry peptide in the composite peptide preparation 4 is 30:10:8:10:15:10:5:15:

20.

13. A food, characterized in that The food comprises the complex peptide composition according to any one of claims 1 to 5 or the complex peptide preparation according to any one of claims 7 to 11.

14. The food according to claim 13, characterized in that The dosage form of the food includes any one or more of solid dosage form, semisolid dosage form and liquid dosage form.

15. The food according to claim 13, characterized in that The food includes: any one or more of candy, soy milk, yogurt, milk, juice, canned food, biscuits, chocolate, cakes, cream, cheese, milk powder, formula milk powder, ice cream, jam, puree, preserved fruit, bread, egg rolls, protein drinks, solid drinks, lactic acid bacteria drinks, carbonated drinks, coffee, jelly, protein bars, energy bars, energy gel, and puffed food.

16. A health product, characterized in that: The health product comprises the complex peptide composition according to any one of claims 1 to 5 or the complex peptide preparation according to any one of claims 7 to 11.

17. The health care product according to claim 16, characterized in that The health care product also includes nutritionally acceptable nutrients.

18. The health care product according to claim 17, characterized in that The nutrients include one or more of dietary fiber, protein, lipids, minerals and vitamins.

19. The health care product according to claim 16, characterized in that The dosage form of the health care product includes any one or more of solid dosage form, semisolid dosage form, and liquid dosage form.

20. The health care product according to claim 19, characterized in that The dosage forms of the health care product include tablets, capsules, soft capsules, granules, pills, gel candies, powders, oral liquids or drops.

21. Use of the complex peptide composition according to any one of claims 1 to 5 or the complex peptide preparation according to any one of claims 7 to 11 in the preparation of a drug for promoting bone and cartilage growth.

22. A medicine, characterized in that: The medicine comprises the complex peptide composition according to any one of claims 1 to 5 or the complex peptide preparation according to any one of claims 7 to 11.

23. The medicine according to claim 22, characterized in that The medicine also includes one or more pharmaceutically acceptable excipients.

24. The medicine according to claim 22, characterized in that The dosage form of the medicine includes a dosage form for gastrointestinal administration or a dosage form for parenteral administration.

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