Anti-aging composition containing DNA sodium and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to an anti-aging composition containing DNA sodium and application of the anti-aging composition. The composition is prepared from DNA sodium, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E and sodium hyaluronate. According to the emulsion disclosed by the invention, various active ingredients such as the DNA sodium, the snake venom tripeptide, the elastin peptide, the low-molecular collagen peptide, the nicotinamide, the alpha-arbutin and the purple blue bletilla striata bulb fermentation liquor are organically combined, so that multiple anti-aging and whitening effects of resisting oxidation, inhibiting melanin synthesis, promoting collagen and elastin generation, improving uneven skin color and the like can be synergistically realized. Purple blue bletilla striata bulbs are subjected to lactobacillus-candida compound fermentation, the product does not contain coarse particles after refined filtration treatment, is clarified, is stable for a long time after being added into an emulsion system, and is not easy to separate out and precipitate or cause system layering. The emulsion is light in structure, not sticky and greasy, endows skin with soft and moist touch feeling, and is suitable for various skin types including sensitive skin, and active ingredients are easy to permeate and absorb.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of daily chemical technology, in particular to an anti-aging composition containing DNA sodium and application thereof. BACKGROUND

[0002] Skin aging is the result of the combined action of natural aging of the human body and external environmental factors, which is manifested as skin relaxation, decreased elasticity, wrinkle production and dark skin color. Oxidative stress, ultraviolet radiation, collagen loss and melanin deposition are considered to be important factors that accelerate skin aging and pigmentation. Therefore, developing skin care compositions with multiple effects to simultaneously address skin aging and pigmentation problems has become a research hotspot in the field of skin care products.

[0003] The existing whitening and anti-aging skin care emulsion has the following problems: single anti-aging ingredient, mostly focused on supplementing collagen or antioxidants, difficult to synergistically act from multiple pathways; insufficient synergy between whitening ingredients (such as single niacinamide or arbutin) and anti-aging ingredients, limited effect; added plant extracts are prone to precipitation in the emulsion due to improper process, affecting the appearance and stability of the product; poor thermal stability of the emulsion, prone to delamination, emulsion breaking and other phenomena under high temperature or freezing conditions.

[0004] DNA sodium, as a water-soluble nucleic acid salt, has been proven to have good antioxidant, cell repair and anti-aging effects, but its application in whitening emulsion has been rarely reported. Bletia hyacinthina bulbs are rich in polysaccharides, phenols and other components, and have the effects of whitening and anti-inflammatory. However, traditional extracts have the problems of poor solubility and insufficient stability, which are not conducive to application in emulsion. Therefore, there is an urgent need for a whitening and anti-aging emulsion with scientific ingredient combination, significant synergistic effect and excellent physical stability. SUMMARY

[0005] To solve the above technical problems, the present application provides an anti-aging composition, which comprises: DNA sodium, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E, sodium hyaluronate.

[0006] As an embodiment of the present application, the composition further comprises: BLETIA HYACINTHINA bulb extract, niacinamide, α-arbutin.

[0007] The present application provides an anti-aging emulsion, the preparation raw material of which comprises the anti-aging composition.

[0008] As an embodiment of the present application, the preparation raw material of the anti-aging emulsion comprises:

[0009] DNA sodium, nicotinamide, alpha-arbutin, gynostemma pentaphyllum extract, bletia hyacinthina bulb extract, gentian root extract, tritopeptide, hydrolyzed elastin, ceramide NP, squalane, jojoba esters, glycerin, 1,3-propanediol, sodium hyaluronate, vitamin E.

[0010] As an embodiment of the present application, the preparation raw material of the anti-aging emulsion further comprises a complex surfactant, a preservative and a solvent.

[0011] As an embodiment of the present application, the complex surfactant comprises: octyldodecanol polyether-16, polyglyceryl-3 methylglucose distearate, phytosteryl / oleate, glyceryl monostearate, PEG-20 stearate, octyldodecanol polyether-6.

[0012] As an embodiment of the present application, the mass ratio of octyldodecanol polyether-16, polyglyceryl-3 methylglucose distearate, phytosteryl / oleate, glyceryl monostearate, PEG-20 stearate, octyldodecanol polyether-6 is (0.80-1.0):(0.70-1.0):(0.50-1.0):(0.30-0.5):(0.30-0.5).

[0013] As an embodiment of the present application, the mass ratio of octyldodecanol polyether-16, polyglyceryl-3 methylglucose distearate, phytosteryl / oleate, glyceryl monostearate, PEG-20 stearate, octyldodecanol polyether-6 is 0.80:0.70:0.50:0.30:0.30.

[0014] As an embodiment of the present application, the preparation method of the bletia hyacinthina bulb extract is as follows:

[0015] (1) 100g of dried bletia hyacinthina bulb powder is ground to 600 mesh, and distilled water is added according to the solid-liquid ratio of 1:11. After reflux extraction at 80℃ for 1h, the filtrate is filtered;

[0016] (2) The pH of the filtrate in step (1) is adjusted to 5.5-6.0, 10wt% inoculum is inoculated, and then fermented at 37℃ for 48-72h. After fermentation, the residue is removed by centrifugation at 4000rpm for 10min, and the supernatant is taken;

[0017] (3) The supernatant is filtered through a 0.45μm microporous membrane to remove bacteria, and the bletia hyacinthina bulb extract is obtained.

[0018] As an embodiment of the present application, the anti-aging emulsion is applied in the field of daily chemicals.

[0019] With the above technical solution, the present application has the following beneficial effects:

[0020] The emulsion of the present application combines DNA sodium, snake venom tripeptide, elastin peptide, low molecular collagen peptide, nicotinamide, alpha-arbutin, and BLETIA HYACINTHINA rhizome ferment, etc. to realize multiple anti-aging and whitening effects such as antioxidation, inhibition of melanin synthesis, promotion of collagen and elastin production, and improvement of uneven skin color. The BLETIA HYACINTHINA rhizome is fermented by lactic acid bacteria and Candida, and the product is treated by precision filtration to remove coarse particles and become clear. The product is stable in the emulsion system for a long time and is not easy to precipitate or cause stratification. The emulsion structure is light and not sticky, which gives the skin a soft and smooth touch, and the active ingredients are easy to penetrate and absorb, which is suitable for various skin types, including sensitive skin. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 Figure 1 is a photograph of the VISIA-CR eye wrinkle before the product test of Example 1.

[0022] Figure 2 Figure 2 is a photograph of the VISIA-CR eye wrinkle after the product test of Example 1. DETAILED DESCRIPTION

[0023] The present application will be further explained and described in conjunction with specific embodiments.

[0024] Table 1 below shows the formulations of specific examples.

[0025] Table 1 Formulations of each example

[0026]

[0027]

[0028] In the above Examples 1-3, the preparation method of the BLETIA HYACINTHINA rhizome extract is as follows:

[0029] (1) 100 g of dried BLETIA HYACINTHINA rhizome powder is ground to 600 mesh, and distilled water is added according to the ratio of 1:11. The mixture is refluxed at 80°C for 1 hour, and then filtered to obtain the filtrate;

[0030] (2) The pH of the filtrate in step (1) is adjusted to 5.5-6.0, and 10 wt% of the inoculum is inoculated, and then fermented at 37°C for 60 hours. After fermentation, the residue is removed by centrifugation at 4000 rpm for 10 min, and the supernatant is taken;

[0031] (3) The supernatant is filtered through a 0.45 μm microporous membrane to remove bacteria to obtain the BLETIA HYACINTHINA bulb extract.

[0032] The bacteria are Lactobacillus plantarum, Candida krusei, and Bacillus subtilis, and the mass ratio is 4:4:2. 24936), Candida krusei (Candida krusei 31749), and Bacillus subtilis (Bacillus subtilis 10732), and the mass ratio is 4:4:2.

[0033] The preparation method of the emulsion provided in Examples 1-3 is as follows:

[0034] The components are grouped as follows:

[0035] Phase A: deionized water, DNA sodium, nicotinamide, α-arbutin, BLETIA HYACINTHINA root extract, gentian root extract, centella asiatica extract, 1,3-propanediol, glycerol, sodium hyaluronate;

[0036] Phase B: ethylhexyl cocoate, jojoba esters, squalane, ceramide NP, vitamin E;

[0037] Phase C: octyldodecanol polyoxyl ether-16, polyglyceryl-3 methylglucose distearate, phytosteryl / oleate, glyceryl monostearate, PEG-20 stearate

[0038] Phase D: snake venom tripeptide, hydrolyzed elastin

[0039] S01 In a clean reaction kettle, add deionized water, glycerol, and 1,3-propanediol, and heat to 60-70°C; add the remaining A-phase ingredients and stir until uniform; maintain stirring and keep the temperature at about 65°C;

[0040] S02 In another container, add the B-phase ingredients, then add the C-phase under stirring; heat to 75°C, completely melt, and stir until uniform;

[0041] S03 Slowly pour the mixture obtained in S02 into the mixture obtained in S01 (about 65°C), and homogenize under high shear (8000-10000 rpm) for 35 minutes; after emulsification, continue stirring for 20 minutes while maintaining the temperature at about 60°C; cool to below 45°C, and add the D-phase while stirring; add a small amount of pH adjusting ingredient (such as sodium citrate) to adjust the pH to 6.0-6.5; stir at room temperature to cool to 30°C, and filter to remove bubbles.

[0042] The present application also provides an embodiment 4, which is different from embodiment 1 in that the strain in the preparation method of the Bletia hyacinthina bulb extract is Lactobacillus plantarum 24936).

[0043] The present application also provides an embodiment 5, which is different from embodiment 1 in that:

[0044] Water, glycerin, 1,3-propanediol, nicotinamide, alpha-arbutin, DNA sodium, and sodium hyaluronate are all added to a container, and stirred and mixed uniformly at room temperature.

[0045] In another container, squalane, ethylhexyl octanoate, jojoba ester, vitamin E, ceramide NP, PEG-20 stearate, Ceteareth-20, and Steareth-2 are added, and stirred at room temperature for 10 minutes.

[0046] The water phase is slowly poured into the oil phase, and directly emulsified and stirred at room temperature (handheld stirrer, about 1000 rpm, 5 minutes). After stirring, the remaining ingredients are added and stirred uniformly.

[0047] Performance test

[0048] Test 1: By storing the emulsions of embodiments 1-5 under different temperature conditions, observe the stability changes within a certain period of time, and predict their quality performance under conventional storage or extreme transportation conditions. The temperatures are: 4℃, 25℃, 45℃, cold and hot cycle (4℃(24h)-45℃(24h), 1 cycle = 48h, cycle 5-10 times). Observe the results after 30 days.

[0049] Table 2 test results of test 1

[0050]

[0051] Test 2: Tyrosinase inhibition rate test method (using L-DOPA as substrate)

[0052] Experimental principle

[0053] Tyrosinase catalyzes the oxidation of L-DOPA (levodopa) to dopaquinone in the presence of oxygen, and its absorbance can be detected at 475nm. If the active ingredient can inhibit the reaction, it indicates that it has the potential to inhibit melanin synthesis.

[0054] Reagent preparation

[0055] Tyrosinase (source: mushroom, activity unit about 3000U / mg)

[0056] L-DOPA solution (1 mg / mL, freshly prepared, keep away from light)

[0057] Kojic acid (positive control, dissolved in PBS, concentration: 0.1 mg / mL)

[0058] PBS buffer (pH 6.8-7.0)

[0059] In 96-well enzyme plates, each group has a volume of 175 μL, as shown in Table 3 below:

[0060] Table 3

[0061] Group Sample Tyrosinase PBS Reaction group 25 μL sample solution 25 μL — Reaction control group 25 μL sample solution — 25 μL Blank group 25 μL PBS 25 μL — Blank control group 25 μL PBS — 25 μL

[0062] Experimental procedure

[0063] Sample addition: The above samples were added to each group. Incubation: The enzyme plates were incubated in a 37°C water bath or incubator for 5 minutes. Reaction initiation: 100 μL of L-DOPA solution was quickly added to each well. Continue reaction: Incubate for another 5 minutes at 37°C; termination / detection: Terminate the reaction (can be placed on ice or directly detected), immediately measure the absorbance A value at 475 nm wavelength.

[0064] Tyrosinase inhibition rate (%) = [(A r0 -A r1 )-(A c0 -A c1 )] / (A r0 -A r1 )*100%

[0065] Wherein:

[0066] A r0 : Blank group (no sample, with enzyme) absorbance.

[0067] A r1 : Reaction group (with sample, with enzyme) absorbance.

[0068] A c0 : Blank control group (no sample, no enzyme) absorbance.

[0069] A r1 : Reaction control group (with sample, no enzyme) absorbance.

[0070] IC 50 determination

[0071] The inhibition rate data at different concentrations were fitted using SPSS or GraphPad Prism; the concentration that inhibits 50% of tyrosinase activity (IC 50 value) IC 50 The smaller the better.

[0072] Table 4 Test results of Test 2

[0073]

[0074]

[0075] Test 3: Human Anti-wrinkle, Firming Efficacy Evaluation Test

[0076] Test Purpose: To verify whether the product has the actual efficacy of lightening crow's feet, improving skin elasticity and firmness after 28 days of continuous use.

[0077] Test sample usage instructions

[0078] Method of use: Take an appropriate amount of the product and apply it evenly to the skin around the eyes in the morning and evening, gently massage along the skin texture until absorbed; usage period: 28 days of continuous use; test area: bilateral canthus area.

[0079] A total of 31 people, 30 people were actually included; 1 male, 29 females, aged 32-65.

[0080] Test environment

[0081] Temperature: 21 ± 1°C

[0082] Humidity: 50 ± 10% RH

[0083] Test instruments:

[0084] PRIMOS-CR three-dimensional skin texture analyzer (crow's feet depth, volume, number, length)

[0085] VISIA-7 image acquisition system (take contrast photos)

[0086] Cutometer Dual MPA580 skin elasticity analyzer (determine R2 and F4 values)

[0087] Test procedure

[0088] (1) Test preparation before use

[0089] The subjects stopped using any efficacious cosmetics or external drugs one week in advance;

[0090] No products were applied after washing the face the night before the test;

[0091] On the test day, only wash the face with water in the morning;

[0092] Enter the constant temperature and humidity chamber, sit and rest for 30 minutes before starting the measurement;

[0093] Measurement items: PRIMOS-CR, VISIA photo, Cutometer (R2, F4 value).

[0094] (2) Test after 28 days of use

[0095] The same cleaning and environmental adaptation (30 minutes) were also performed; the above-mentioned items were re-measured.

[0096] Crows feet average depth test: average improvement rate = (pre-use mean - post-use mean) / (pre-use mean) x 100%.

[0097] Crows feet number test: average improvement rate = (pre-use mean - post-use mean) / (pre-use mean) x 100%.

[0098] Crows feet volume test: average improvement rate = (pre-use mean - post-use mean) / (pre-use mean) x 100%.

[0099] Crows feet length test: average improvement rate = (pre-use mean - post-use mean) / (pre-use mean) x 100%.

[0100] Skin elasticity R2 value test: average improvement rate = (post-use mean - pre-use mean) / (pre-use mean) x 100%.

[0101] Skin firmness F4 value test: average improvement rate = (pre-use mean - post-use mean) / (pre-use mean) x 100%.

[0102] All data were the average of 3 repeated measurements; paired t-test was performed using SPSS statistical software, p < 0.05 was considered statistically significant; the test results were reported in terms of "improvement rate", "effective rate" and "statistical significance".

[0103] Table 5 Results table of test 3

[0104]

[0105] In the drawings, Figure 1 is the VISIA-CR corner wrinkle photo before the product test of Example 1; Figure 2 is the VISIA-CR corner wrinkle photo after the product test of Example 1;

[0106] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and are not intended to limit the present application; although the present application has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions recorded in the above embodiments can be modified, or some or all of the technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. An anti-aging composition containing DNA sodium, characterized in that, The composition comprises: sodium DNA, hydrolyzed elastin, collagen, snake venom tripeptide, vitamin E, and sodium hyaluronate.

2. The anti-aging composition containing DNA sodium according to claim 1, characterized in that: The composition further comprises: Bletia hyacinthina bulb extract, niacinamide and α-arbutin.

3. An anti-aging lotion, characterized in that: The raw materials for its preparation include the anti-aging composition according to claim 1 or 2.

4. The anti-aging lotion according to claim 3, characterized in that The raw materials for its preparation include: Sodium DNA, Niacinamide, α-Arbutin, Centella Asiatica Extract, Bletia Hyacinthina Bulb Extract, Gentiana Root Extract, Snake Venom Tripeptide, Hydrolyzed Elastin, Ceramide NP, Squalane, Jojoba Esters, Glycerin, 1,3-Propanediol, Sodium Hyaluronate, Vitamin E.

5. The anti-aging lotion according to claim 3, characterized in that The preparation raw materials also include a compound surfactant, a preservative and a solvent.

6. The anti-aging lotion according to claim 5, characterized in that The compound surfactant comprises: octyldodecanol polyether-16, polyglyceryl-3 methyl glucose distearate, phytosterol / oleate, glyceryl monostearate, PEG-20 stearate, and octyldodecanol polyether-6.

7. The anti-aging lotion according to claim 6, characterized in that The mass ratio of the octyl dodecanol polyether-16, polyglyceryl-3 methyl glucose distearate, phytosterol / oleate, glyceryl monostearate, PEG-20 stearate, and octyl dodecanol polyether-6 is (0.80-1.0):(0.70-1.0):(0.50-1.0):(0.30-0.5):(0.30-0.5).

8. The anti-aging lotion according to claim 6, characterized in that The mass ratio of the octyl dodecanol polyether-16, polyglyceryl-3 methyl glucose distearate, phytosterol / oleate, glyceryl monostearate, PEG-20 stearate, and octyl dodecanol polyether-6 is 0.80:0.70:0.50:0.30:0.

30.

9. The anti-aging lotion according to claim 3, characterized in that The preparation method of the Bletia hyacinthina bulb extract is as follows: (1) Grind 100 g of dried Bletilla striata bulb powder through 600 mesh, add distilled water at a ratio of 1:11, reflux extract at 80°C for 1 h, and filter to obtain the filtrate; (2) adjusting the pH of the filtrate of step (1) to 5.5-6.0, inoculating 10 wt% of the bacterial strain, and then fermenting at 37° C. for 48-72 hours; after the fermentation is completed, centrifuging at 4000 rpm for 10 minutes to remove the residue and taking the supernatant; (3) The supernatant is sterilized by fine filtration using a 0.45 μm microporous membrane to obtain the Bletia hyacinthina bulb extract.

10. The anti-aging lotion according to any one of claims 3 to 9, characterized in that Used in daily chemical field.

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