Sewage treatment method based on synchronous short-cut nitrification-denitrification of strain culture

Abstract

The invention relates to the technical field of sewage treatment, in particular to a synchronous short-cut nitrification-denitrification sewage treatment method based on strain culture, which comprises the following steps: (1) respectively inoculating short-cut nitrifying bacteria and denitrifying bacteria into a liquid culture medium A and a liquid culture medium B, and carrying out strain amplification culture to obtain a short-cut nitrifying bacteria solution and a denitrifying bacteria solution; the short-cut nitrifying bacteria comprise air bacillus E4 of which the preservation number is CCTCC No: M 2025641; (2) respectively introducing a short-cut nitrification bacterium solution and a denitrification bacterium solution into a short-cut nitrification device and a denitrification device, and carrying out microbial biofilm formation; and (3) introducing to-be-treated sewage into the short-cut nitrification device for short-cut nitrification treatment, then introducing the to-be-treated sewage into the denitrification device for denitrification treatment, synchronously performing the step (1) during the period, and respectively supplementing the short-cut nitrification bacterium liquid and the denitrification bacterium liquid into the short-cut nitrification device and the denitrification device. According to the method, the strain amplification culture and the short-cut nitrification-denitrification treatment process of the sewage are synchronously carried out, and the new short-cut nitrification strain is matched, so that the strain demand quantity can be reduced, and the sewage treatment effect is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and in particular to a sewage treatment method based on bacterial culture and synchronous short-range nitrification-denitrification. Background Art

[0002] Traditional wastewater microbial denitrification technology first oxidizes ammonia nitrogen into nitrite nitrogen, which is then further oxidized into nitrate nitrogen before being converted into nitrogen gas through denitrification. This technology has the problems of high oxygen and carbon source demand, long wastewater treatment time, high operating energy consumption and cost. Short-range nitrification-denitrification technology, as a new biological denitrification technology, has received widespread attention in the wastewater treatment industry in recent years. Based on traditional microbial denitrification technology, it directly converts nitrate nitrogen into nitrogen gas through denitrification by inhibiting the complete oxidation pathway during nitrification, omitting the process of converting nitrate nitrogen into nitrite nitrogen. This can reduce energy consumption in the wastewater treatment process, reduce the consumption of oxygen and carbon sources, and improve wastewater treatment efficiency.

[0003] The core of short-term nitrification-denitrification technology lies in stopping the oxidation of ammonia nitrogen at the nitrite stage, preventing further oxidation of nitrite to nitrate. However, the current availability of bacterial strains capable of achieving this function is limited. Existing strains often have strict temperature and pH control requirements, can effectively treat low ammonia nitrogen concentrations, and require a large amount of strains. These limitations have limited the widespread application of short-term nitrification-denitrification denitrification technology. Improving short-term nitrification-denitrification technology for wastewater and developing more microbial strains capable of achieving short-term nitrification will help promote the development of new biological denitrification technologies.

[0004] In addition, Bacillus aeruginosa ( Bacillus aerius ) belongs to the genus Bacillus of the phylum Firmicutes, and its type strain is Bacillus aerius 24K(T) AJ831843. Currently, strains within this species are primarily used for biological control of plant diseases, such as Aerobacillus T-026 (patent CN116855400A), which controls pine wilt disease; Aerobacillus A3-5 (patent CN119144476A), which controls tobacco mosaic disease; and Aerobacillus (CN111808776A), which controls corn sheath blight. However, strains within this species with short-range nitrification have yet to be found. Summary of the Invention

[0005] Therefore, the present application provides a wastewater treatment method based on simultaneous short-cut nitrification-denitrification of strain culture.

[0006] Specifically, the present application provides the following technical solutions: Firstly, the present application provides a wastewater treatment method based on simultaneous short-cut nitrification-denitrification of strain culture, comprising the following steps: (1) inoculating short-cut nitrifying bacteria and denitrifying bacteria into liquid culture medium A and liquid culture medium B respectively, and carrying out strain expansion culture to obtain short-cut nitrifying bacteria liquid and denitrifying bacteria liquid; the short-cut nitrifying bacteria comprises air Bacillus E4 with preservation number CCTCC No: M 2025641; Bacillus aerius (2) passing the short-cut nitrifying bacteria liquid and the denitrifying bacteria liquid into a short-cut nitrification device and a denitrification device respectively, and carrying out microbial membrane formation; (3) after completing the microbial membrane formation, passing the wastewater to be treated into the short-cut nitrification device, carrying out short-cut nitrification treatment, and then passing it into the denitrification device, carrying out denitrification treatment, during which step (1) is simultaneously carried out, and the short-cut nitrifying bacteria liquid and the denitrifying bacteria liquid are supplemented into the short-cut nitrification device and the denitrification device respectively. In the present application, the process of strain expansion culture is simultaneously carried out with the process of wastewater short-cut nitrification-denitrification treatment, so that the strain after expansion culture can be added into the wastewater treatment system in time and at any time. Compared with the traditional method of directly adding the purchased strain into the wastewater treatment, the present application can solve the problems of untimely strain addition and large strain demand, and only a small amount of strain and liquid culture medium are needed to realize timely expansion culture.

[0007] In the present application, air Bacillus E4 is used for short-cut nitrification treatment of wastewater. The strain is a new strain isolated from municipal wastewater sludge samples by the team of the present application, which can efficiently convert ammonia nitrogen into nitrite nitrogen, and can limit the ammonia nitrogen oxidation process to the nitrite nitrogen stage, and will not further oxidize the nitrite nitrogen into nitrate nitrogen. In addition, when used for wastewater treatment, the strain can adapt to higher ammonia nitrogen concentration and wider pH and temperature ranges, thus helping to improve the impact load resistance of the wastewater treatment system.

[0008]

[0009] ​Preferably, before step (2), a microbial electrode loaded with Shewanella is used to detect the toxicity and biochemical oxygen demand of the wastewater to be treated, and one or more of the water inlet flow rate, aeration volume, carbon source dosage, short-cut nitrification bacteria solution dosage and denitrification bacteria solution dosage in the short-cut nitrification device and the denitrification device are regulated according to the detection results.

[0010] The microbial electrode uses the principle that Shewanella can discharge electricity, and the difference in the electrical signals released when the sewage is in a toxic and non-toxic state is used to detect the presence of toxicity in the influent, helping to implement emergency measures for sewage treatment in advance. Unlike traditional methods that indirectly reflect the microbial activity of biological systems through measuring water quality indicators such as COD, ammonia nitrogen, and total phosphorus, the present invention uses microbial electrodes to directly reflect the survival status of microorganisms in the biochemical system and can measure BOD in real time. The microbial electrode can display different electrical values ​​according to the level of BOD in the influent. After a long period of simulation (about 3 months) with real BOD data, a fitting curve of the electrical signal and BOD can be simulated, thereby using the microbial electrode to monitor the BOD data in the influent in real time. Through this method, the detection module can be used to obtain the toxicity and biodegradability of the influent in advance, and the sewage treatment system can be regulated accordingly. It can better cope with the toxicity of different sewage on short-range nitrifying and denitrifying bacteria, improve the sewage treatment system's resistance to shock loads, and maintain the sewage treatment effect at a stable level.

[0011] Preferably, the shortcut nitrification device, denitrification device and microbial electrodes are all connected to an intelligent remote feedback module, which regulates one or more of the water inlet flow rate, aeration volume, carbon source dosage, shortcut nitrifying bacteria solution dosage and denitrifying bacteria solution dosage in the shortcut nitrification device and denitrification device according to the toxicity and biochemical oxygen demand measured by the microbial electrodes and the sewage treatment conditions in the shortcut nitrification device and denitrification device.

[0012] Preferably, in step (1), the short-range nitrifying bacteria further include Klebsiella grimoni with a deposit number of CCTCC No: M 2025642 ( Klebsiella grimontii )A5.

[0013] When using the metabolic activities of microorganisms for sewage treatment, complex interactions occur between different strains, manifesting in forms such as antagonism and synergy. Klebsiella grimoni A5 is another new strain isolated by the present invention team from municipal sewage sludge samples. When used alone, it can also oxidize ammonia nitrogen into nitrite nitrogen and prevent nitrite nitrogen from being further oxidized into nitrate nitrogen. The present invention found that when two specific strains, Bacillus aerogenes E4 and Klebsiella grimoni A5, are used together, a synergistic effect can be generated between the two, and the short-range denitrification efficiency of sewage is significantly higher than that of either strain alone.

[0014] Preferably, in step (1), when the short-range nitrifying bacteria are inoculated into the liquid culture medium A, the ratio of the number of viable bacteria of the inoculated Bacillus aeruginosa E4 to that of Klebsiella grimundi A5 is 1:0.6-0.9, and the total inoculation amount is 2×10 6 ~8×10 6 CFU / mL.

[0015] Preferably, in step (1), the liquid culture medium A is TSB medium (Tryptic Soy Broth); the conditions for expanding the culture of short-range nitrifying bacteria are: dissolved oxygen content of 1-4 mg / L, temperature of 35-40°C, and pH of 7.0-7.5.

[0016] Preferably, in step (1), the liquid culture medium B comprises the following components at the following concentrations: KNO3 1-2 g / L, sodium citrate 6-8 g / L, KH2PO4 0.8-1.2 g / L, MgSO4·7H2O 0.1-0.3 g / L, FeSO4·7H2O 0.01-0.05 g / L, and CaCl2·2H2O 0.01-0.03 g / L; and the conditions for expanding the culture of denitrifying bacteria are: dissolved oxygen content not higher than 0.2 mg / L, temperature of 30-35°C, and pH of 7.0-7.5.

[0017] Second, the present invention provides a strain of air Bacillus ( Bacillus aerius ), the strain name of the aerobic Bacillus is E4, which is deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with the deposit number CCTCCNo: M 2025641.

[0018] Third, the present invention provides a composite bacterial agent with ammonia oxidation function, including the aerobic Bacillus and Klebsiella Grimondii ( Klebsiella grimontii The strain name of the Klebsiella grimoni is A5, which is deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with a deposit number of CCTCC No: M2025642.

[0019] Fourthly, the present invention provides a method for preparing the composite bacterial agent, comprising the following steps: inoculating the aerobic Bacillus and Klebsiella grierii into a liquid culture medium for expansion culture.

[0020] Preferably, the liquid culture medium is TSB medium.

[0021] Preferably, during the inoculation process, the ratio of the number of viable bacteria of the inoculated Bacillus aeruginosa to that of the inoculated Klebsiella grimundi is 1:0.6-0.9.

[0022] As preferred, the total inoculation amount of the Bacillus aerius and the Klebsiella grycomyces in the liquid culture medium is 2×10 6 ~8×10 6 CFU / mL.

[0023] Fifthly, the application provides the application of the Bacillus aerius or the compound microbial agent in the short-cut nitrification treatment of sewage.

[0024] Compared with the prior art, the application has the following advantages: (1) The application can add the strains which have completed the expansion culture to the sewage treatment system in time by synchronously performing the expansion culture process and the short-cut nitrification-denitrification treatment process of sewage, thereby solving the problem of the traditional method that the strains are not added in time and the large demand for the strains.

[0025] (2) Before the short-cut nitrification-denitrification treatment of sewage, the toxicity and the biochemical oxygen demand of the sewage are detected by using the microbial electrode, the sewage treatment system is regulated according to the detection results, the toxicity of different sewage to the short-cut nitrification bacteria and the denitrification bacteria can be better coped with, the impact load resistance of the sewage treatment system is improved, and the sewage treatment effect is maintained at a stable level.

[0026] (3) The intelligent remote feedback module is used, the toxicity and the chemical oxygen demand measured by the microbial electrode and the real-time treatment effect of the sewage are used as the basis, timely emergency measures are taken, the inflow, the aeration amount, the carbon source addition amount and the strain liquid addition amount are regulated in real time, the sewage treatment effect is improved, and the accurate calculation of the carbon source and the strain liquid addition amount is realized, thereby saving the carbon source and the strains.

[0027] (4) The Bacillus aerius E4 in the application can realize the conversion of ammonia nitrogen to nitrite nitrogen, and limit the ammonia nitrogen oxidation process to the nitrite nitrogen stage, and will not further oxidize the nitrite nitrogen to nitrate nitrogen, can play a good role in the short-cut nitrification treatment of sewage, and this is the first time to find a strain with the above function in the Bacillus aerius. Bacillus aerius (5) The compound microbial agent composed of the Bacillus aerius E4 and the Klebsiella grycomyces A5 is used, when used in the short-cut nitrification treatment of sewage, the two strains can produce a synergistic effect, and effectively improve the efficiency of converting ammonia nitrogen to nitrite nitrogen.

[0028] (5) The compound microbial agent composed of the Bacillus aerius E4 and the Klebsiella grycomyces A5 is used, when used in the short-cut nitrification treatment of sewage, the two strains can produce a synergistic effect, and effectively improve the efficiency of converting ammonia nitrogen to nitrite nitrogen. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is the result graph of the Griess test in Example 1. Wherein: Figure 1 (A) is the photo of the negative control group after adding the Griess reagent; Figure 1(B) is a photo of the positive control group after adding Griess reagent; Figure 1 (C) is a photo of the experimental group of Bacillus aeruginosa E4 after the addition of Griess reagent; Figure 1 (D) A photograph of the Klebsiella A5 experimental group after the addition of Griess reagent.

[0030] Biomaterial deposit information The bacterial strain aerobacillus of the present invention ( Bacillus aerius ) E4 is deposited in the China Center for Type Culture Collection (CCTCC), the depository address is Wuhan University, Wuhan, China, the deposit number is CCTCC No: M 2025641, and the deposit date is March 31, 2025.

[0031] The strain Klebsiella grimundi of the present invention ( Klebsiella grimontii ) A5 is deposited in the China Center for Type Culture Collection (CCTCC), the depository address is Wuhan University, Wuhan, China, the deposit number is CCTCC No: M2025642, and the deposit date is March 31, 2025. DETAILED DESCRIPTION

[0032] The present invention will be further described below in conjunction with specific examples. The following examples can enable professionals in this field to more fully understand the present invention, but are not intended to limit the present invention in any way.

[0033] The bacterial strain aerobic Bacillus involved in the present invention ( Bacillus aerius ) E4 was isolated from a municipal sewage sludge sample, and its 16S rRNA sequence is shown in SEQ ID NO. 1. The specific sequence is as follows:

[0034] The Klebsiella grycomyces strain involved in the present application is Klebsiella grimontii A5 is isolated from a municipal sewage sludge sample, and its 16S rRNA sequence is shown as SEQ ID NO. 2, and the specific sequence is as follows:

[0035] Example 1: Short-term nitrification function verification of strains (Griess test) According to the following formula, aerobic ZPM14 liquid medium is prepared: (NH4)2SO42.0 g / L, K2HPO41.0 g / L, MgSO40.5 g / L, CaCO31.0 g / L, NaCl 10.0 g / L, and the solvent is purified water; pH = 7.0; 121°C high temperature wet heat sterilization for 15 min.

[0036] Griess reagent can be used to detect nitrite. When there is nitrite ion in the system, Griess reagent will react with nitrite ion to generate red azo compound. Based on this, in this embodiment, Griess reagent is used to detect whether the strain can convert ammonia nitrogen into nitrite nitrogen. The experimental grouping design is as follows: (1) Negative control group: 400 μL of Griess reagent was added to a centrifuge tube containing 5 mL of aerobic ZPM14 liquid medium.

[0037] (2) Positive control group: 400 μL of Griess reagent was added to a centrifuge tube containing 5 mL of 6.4×10 -6 mol / L sodium nitrite solution.

[0038] (3) Bacillus aerius E4 experimental group: single colony of Bacillus aerius E4 was picked and inoculated into aerobic ZPM14 liquid medium, and cultured at 37°C for 2 days in an aerobic environment to obtain primary culture liquid. The primary culture liquid was aspirated and inoculated into a centrifuge tube containing 5 mL of aerobic ZPM14 liquid medium, and cultured at 37°C for 3 days in an aerobic environment. Then 400 μL of Griess reagent was added, and the color change of the liquid in the centrifuge tube was observed.

[0039] (4) Klebsiella grimontii A5 experimental group: single colony of Klebsiella grimontii A5 was picked and inoculated into aerobic ZPM14 liquid medium, and cultured at 37°C for 2 days in an aerobic environment to obtain primary culture liquid. The primary culture liquid was aspirated and inoculated into a centrifuge tube containing 5 mL of aerobic ZPM14 liquid medium, and cultured at 37°C for 3 days in an aerobic environment. Then 400 μL of Griess reagent was added, and the color change of the liquid in the centrifuge tube was observed.

[0040] The experimental results are as follows: in the negative control group, as shown in Figure 1 (A), the liquid in the centrifuge tube was still colorless and transparent after the addition of Griess reagent, and there was no color change; in the positive control group, as shown in Figure 1(B) shows that the liquid in the centrifuge tube changes from colorless and transparent to purple red after the addition of Griess reagent; in the experimental groups of Bacillus aerius E4 and Klebsiella grycomyces A5, as shown in Figure 1 (C) and Figure 1 (D) shows that the liquid in the centrifuge tube changes from colorless and transparent to purple red after the addition of Griess reagent. The above results show that Bacillus aerius E4 and Klebsiella grycomyces A5 can both convert ammonia nitrogen into nitrite nitrogen.

[0041] Example 2: Short-term nitrification ability of Bacillus aerius E4 under different conditions 2.1 Test design A single colony of Bacillus aerius E4 was picked and inoculated into TSB medium, which was cultured at 37°C in an aerobic environment to the logarithmic growth phase to obtain Bacillus aerius E4 seed liquid.

[0042] According to the following formula, aerobic ZPM14 liquid medium was prepared: (NH4)2SO4 100~2000 mg / L (calculated as ammonia nitrogen concentration), K2HPO4 1.0 g / L, MgSO4 0.5 g / L, CaCO3 1.0 g / L, NaCl 10.0 g / L, and the solvent was purified water; pH=5.0~9.0; 121°C high temperature and humidity sterilization for 15 min.

[0043] According to a 3% inoculation amount, Bacillus aerius E4 seed liquid was inoculated into aerobic ZPM14 liquid medium, which was cultured at 10~40°C in an aerobic environment for 3 days, and then the ammonia nitrogen, nitrite nitrogen and total nitrogen concentrations in the medium were detected.

[0044] 2.2 Test results 2.2.1 Short-term nitrification ability of Bacillus aerius E4 under different initial ammonia nitrogen concentrations When the initial pH of the aerobic ZPM14 liquid medium was 8.0 and the culture temperature was 37°C, the initial ammonia nitrogen concentrations of the aerobic ZPM14 liquid medium were 100, 200 and 2000 mg / L, respectively, and the ammonia nitrogen, nitrite nitrogen and total nitrogen concentrations measured after 3 days of culture are shown in Table 1.

[0045] Table 1 Short-term nitrification ability under different initial ammonia nitrogen concentrations 2.2.2 Short-term nitrification ability of Bacillus aerius E4 under different initial pH When the initial ammonia nitrogen concentration of the aerobic ZPM14 liquid medium was 2000 mg / L, and the initial pH of the aerobic ZPM14 liquid medium was 5.0, 6.0, 7.0, 8.0 and 9.0 respectively, the ammonia nitrogen, nitrite nitrogen and total nitrogen concentrations measured after 3 days of culture are shown in Table 2.

[0046] Table 2 Short-cut nitrification capacity at different initial pH 2.2.3 Short-cut nitrification capacity of Aeribacillus aerolysinus E4 at different initial temperatures When the initial ammonia nitrogen concentration of the aerobic ZPM14 liquid medium was 2000 mg / L, and the initial pH of the aerobic ZPM14 liquid medium was 8.0, the ammonia nitrogen, nitrite nitrogen and total nitrogen concentrations measured after 3 days of culture at culture temperatures of 10°C, 20°C, 37°C and 40°C respectively are shown in Table 3.

[0047] Table 3 Short-cut nitrification capacity at different temperatures Example 3: Synergistic effect of Aeribacillus aerolysinus E4 and Klebsiella grycomyces A5 Aeribacillus aerolysinus E4 powder was inoculated into TSB medium at an inoculation amount of 5 x 10 6 CFU / mL, and cultured at 37°C for 3 days in an aerobic environment to obtain seed solution I.

[0048] Klebsiella grycomyces A5 powder was inoculated into TSB medium at an inoculation amount of 5 x 10 6 CFU / mL, and cultured at 37°C for 3 days in an aerobic environment to obtain seed solution II.

[0049] Aeribacillus aerolysinus E4 powder and Klebsiella grycomyces A5 powder were inoculated into TSB medium at inoculation amounts of 3.1 x 10 6 CFU / mL and 1.9 x 10 6 CFU / mL respectively, and cultured at 37°C for 3 days in an aerobic environment to obtain seed solution III.

[0050] Aeribacillus aerolysinus E4 powder and Klebsiella grycomyces A5 powder were inoculated into TSB medium at inoculation amounts of 2.7 x 10 6 CFU / mL and 2.3 x 10 6 CFU / mL respectively, and cultured at 37°C for 3 days in an aerobic environment to obtain seed solution IV.

[0051] According to the following formula, the aerobic ZPM14 liquid medium is prepared: (NH4)2SO42000 mg / L (calculated as ammonia nitrogen concentration), K2HPO41.0 g / L, MgSO40.5 g / L, CaCO31.0 g / L, NaCl 10.0 g / L, and the solvent is purified water; pH = 8.0; 121 ℃ high temperature and heat sterilization for 15 min.

[0052] According to the inoculation amount of 3%, seed liquid I, seed liquid II, seed liquid III and seed liquid IV are inoculated into the aerobic ZPM14 liquid medium, respectively, and after 2 days of culture at 37 ℃ in an aerobic environment, the ammonia nitrogen, nitrite nitrogen and total nitrogen concentrations in the medium are detected, and the results are shown in Table 4.

[0053] Table 4 Short-cut nitrification ability of single bacteria and compound bacterial agent Analysis of detection results: As can be seen from Table 4, compared with the use of air Bacillus E4 and Klebsiella A5 alone (seed liquid I and seed liquid II), the use of the compound bacterial agent of the two strains (seed liquid III and seed liquid IV) can effectively improve the efficiency of converting ammonia nitrogen into nitrite nitrogen, which indicates that the two strains can produce synergistic effect in short-cut nitrification.

[0054] Example 4: Sewage treatment based on strain culture and simultaneous short-cut nitrification-denitrification The following sewage treatment system is constructed: composed of a detection module, a strain culture and simultaneous short-cut nitrification-denitrification module, and an intelligent remote feedback module. The strain culture and simultaneous short-cut nitrification-denitrification module includes a short-cut nitrifying bacteria culture device, a denitrifying bacteria culture device, an aerobic tank and an anaerobic tank. The aerobic tank and the anaerobic tank are both filled with polyurethane biological filler, and the filling rate is 20%.

[0055] Using the above sewage treatment system, the sewage is treated according to the following steps (the test water is urban domestic sewage, and the water quality is shown in Table 5, and the pH is 7.4): (1) TSB medium is added to the short-cut nitrifying bacteria culture device, and then air Bacillus E4 powder and Klebsiella A5 powder are inoculated into it, and the inoculation amount of the two strains is 2.7×10 6 CFU / mL and 2.3×10 6 CFU / mL, respectively. The temperature in the short-cut nitrifying bacteria culture device is controlled at 35~40 ℃, the dissolved oxygen content is 1~3 mg / L, and the pH is 7.0~7.5, for strain expansion culture.

[0056] (2) Prepare the denitrifying bacteria culture solution according to the following formula: KNO31.5 g / L, sodium citrate 7 g / L, KH2PO41 g / L, MgSO4·7H2O 0.2 g / L, FeSO4·7H2O 0.03 g / L, CaCl2·2H2O 0.02 g / L. Add the denitrifying bacteria culture solution into the denitrifying bacteria culture device, and then inoculate the denitrifying bacteria powder (purchased from Zelin Environmental Protection) into the device, with an inoculation amount of 3×10 6 CFU / mL. Control the temperature in the denitrifying bacteria culture device at 30-35℃, the dissolved oxygen content at not higher than 0.2 mg / L, and the pH at 7.0-7.5, to carry out the strain expansion culture.

[0057] (3) Add the short-cut nitrifying bacteria solution obtained after the strain expansion culture in step (1) into the aerobic tank to carry out the biofilm formation; and add the denitrifying bacteria solution obtained after the strain expansion culture in step (2) into the anaerobic tank to carry out the biofilm formation.

[0058] (4) Detect the toxicity and biochemical oxygen demand of the wastewater to be treated by using the Shewanella-loaded microbial electrode, and input the detection results into the intelligent remote feedback module.

[0059] (5) Pass the wastewater to be treated after the microbial electrode detection into the aerobic tank after the biofilm formation, to carry out the short-cut nitrification treatment, during which the dissolved oxygen content in the aerobic tank is controlled at 0.4-0.5 mg / L, and the hydraulic retention time in the aerobic tank is 12 h; and then pass the effluent of the aerobic tank into the anaerobic tank after the biofilm formation, to carry out the denitrification treatment, during which the dissolved oxygen content in the anaerobic tank is controlled at not higher than 0.2 mg / L, and the hydraulic retention time in the anaerobic tank is 10 h. In the process of the above short-cut nitrification and denitrification treatment, step (1) is carried out synchronously, and the effluent water quality of the aerobic tank and the anaerobic tank is monitored, and the detection results are input into the intelligent remote feedback module.

[0060] (6) Through the intelligent remote feedback module, the influent flow, the aeration amount, the sodium acetate addition amount and the short-cut nitrifying bacteria solution addition amount in the short-cut nitrification device, and the denitrifying bacteria solution addition amount in the denitrification device are regulated and controlled according to the toxicity and biochemical oxygen demand measured by the microbial electrode and the effluent water quality of the aerobic tank and the anaerobic tank.

[0061] During the stable operation of the wastewater treatment system, the effluent water quality of the anaerobic tank is monitored, and the results are shown in Table 5 (average value).

[0062] Table 5 Wastewater treatment effect based on strain culture synchronous short-cut nitrification-denitrification The detection result analysis shows that after the sewage is subjected to the short-cut nitrification treatment by the composite microbial inoculum composed of the bacillus aerius E4 and the klebsiella mobilis A5 and then subjected to the denitrification treatment by the denitrification bacteria, the ammonia nitrogen concentration in the sewage can be effectively reduced (the ammonia nitrogen removal rate can reach 90% or more), and the organic matters in the sewage can be degraded, and the COD and BOD values of the sewage are reduced.

[0063] Finally, it should be noted that the above enumeration is only a specific embodiment of the present application. Obviously, the present application is not limited to the above embodiment, but can have many variations. All variations that can be directly derived or inferred from the content disclosed in the present application by those of ordinary skill in the art should be considered as falling within the protection scope of the present application.

Claims

1. A sewage treatment method based on bacterial culture and synchronous short-cut nitrification-denitrification, characterized in that: The following steps are involved: (1) Inoculating short-range nitrifying bacteria and denitrifying bacteria into liquid culture medium A and liquid culture medium B respectively, and performing bacterial expansion culture to obtain short-range nitrifying bacteria liquid and denitrifying bacteria liquid; the short-range nitrifying bacteria include Bacillus aeruginosa with a preservation number of CCTCC No: M2025641 ( Bacillus aerius )E4; (2) The short-range nitrifying bacteria solution and the denitrifying bacteria solution are respectively introduced into the short-range nitrification device and the denitrification device to form microbial biofilm; (3) After the completion of microbial biofilm formation, the sewage to be treated is passed into the short-range nitrification device for short-range nitrification treatment, and then passed into the denitrification device for denitrification treatment. During this period, step (1) is carried out simultaneously, and the short-range nitrification bacteria liquid and denitrification bacteria liquid are respectively added to the short-range nitrification device and the denitrification device.

2. The sewage treatment method according to claim 1, characterized in that: Prior to step (2), a microbial electrode loaded with Shewanella is used to detect the toxicity and biochemical oxygen demand of the wastewater to be treated, and one or more of the water inlet flow rate, aeration volume, carbon source dosage, short-range nitrification bacteria solution dosage, and denitrification bacteria solution dosage in the short-range nitrification device and the denitrification device are regulated based on the detection results.

3. The sewage treatment method according to claim 1, characterized in that: The short-cut nitrification device, denitrification device and microbial electrodes are all connected to an intelligent remote feedback module. The intelligent remote feedback module regulates one or more of the water inlet flow, aeration volume, carbon source dosage, short-cut nitrification bacteria solution dosage and denitrification bacteria solution dosage in the short-cut nitrification device and denitrification device according to the toxicity and biochemical oxygen demand measured by the microbial electrodes and the sewage treatment conditions in the short-cut nitrification device and denitrification device.

4. The sewage treatment method according to claim 1, characterized in that: In step (1), the short-range nitrifying bacteria also include Klebsiella grimoniae with a preservation number of CCTCC No: M 2025642 ( Klebsiella grimontii )A5.

5. The sewage treatment method according to claim 4, characterized in that: In step (1), when the short-range nitrifying bacteria are inoculated into the liquid culture medium A, the ratio of the number of viable bacteria of the inoculated Bacillus aeruginosa E4 to that of Klebsiella grimundi A5 is 1:0.6-0.9, and the total inoculation amount is 2×10 6 ~8×10 6 CFU / mL.

6. The sewage treatment method according to claim 1 or 4, characterized in that: In step (1), the liquid culture medium A is TSB culture medium; the conditions for expanding the culture of short-range nitrifying bacteria are: dissolved oxygen content of 1-4 mg / L, temperature of 35-40°C, and pH of 7.0-7.

5.

7. The sewage treatment method according to claim 1, characterized in that: In step (1), the liquid culture medium B includes the following components in concentrations: KNO3 1~2 g / L, sodium citrate 6~8 g / L, KH2PO4 0.8~1.2 g / L, MgSO4·7H2O 0.1~0.3 g / L, FeSO4·7H2O 0.01~0.05 g / L, CaCl2·2H2O 0.01~0.03 g / L; the conditions for expanding the culture of denitrifying bacteria are: dissolved oxygen content not higher than 0.2 mg / L, temperature of 30~35°C, and pH of 7.0~7.

5.

8. A strain of airborne Bacillus ( Bacillus aerius ), characterized in that, The strain of Bacillus aeruginosa is named E4 and is deposited in China Center for Type Culture Collection with a deposit number of CCTCC No: M 2025641.

9. A composite bacterial agent with ammonia oxidation function, characterized in that: comprising the air bacillus as claimed in claim 8, and further comprising Klebsiella grimonii ( Klebsiella grimontii The strain name of the Klebsiella grimoni is A5, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC No: M 2025642.

10. Use of the air bacillus according to claim 8 or the composite bacterial agent according to claim 9 in the short-cut nitrification treatment of sewage.

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