Device and method for real-time online monitoring of tetracycline

Abstract

The invention provides a real-time on-line tetracycline monitoring device and method.The device comprises a box body, a rotary drum, a driving mechanism, a supporting plate, a monochromator, a quantitative liquid inlet mechanism and a main control unit, a reaction cylinder is arranged in the box body, a transparent window is arranged on the side face of the reaction cylinder, the top and the bottom of the reaction cylinder are connected with a sample inlet pipe and a drain valve respectively, and a liquid pump is arranged on the sample inlet pipe; a working electrode and a counter electrode are arranged in the reaction cylinder, the rotating cylinder rotatably sleeves the outer side of the reaction cylinder, light holes are uniformly distributed in the side surface of the rotating cylinder around the axis of the rotating cylinder, the driving mechanism is in driving connection with the rotating cylinder, the monochromator is located on one side of the rotating cylinder, and the quantitative liquid inlet mechanism is arranged on the top surface of the box body and is communicated with the reaction cylinder; the main control unit comprises an extended gate transistor, a current signal acquisition unit, an analysis unit and a storage unit; and a gate of the extended gate transistor is electrically connected with the working electrode. The monochromator and the expanded gate field effect transistor are arranged, and the online monitoring function of the concentration of the tetracycline solution is achieved by analyzing photoelectric fingerprint data of solutions with different concentrations.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of tetracycline detection, in particular to a device and method for real-time online monitoring of tetracycline. BACKGROUND

[0002] Tetracycline is a broad-spectrum antibiotic with a pina four benzene mother nucleus structure, which is yellow in appearance and slightly soluble in water. It exerts antibacterial effect by inhibiting bacterial protein synthesis, and is effective against gram-positive bacteria, gram-negative bacteria and some mycoplasma and chlamydia. At present, it is widely used in medical clinics and livestock breeding industry. However, the problem of tetracycline residues in the environment and food is becoming increasingly serious, long-term exposure can induce human drug resistance gene expression, cause allergic reactions, and accumulate in soil and water, causing ecological pollution.

[0003] In the prior art, the detection methods of tetracycline mainly include liquid chromatography-mass spectrometry, high performance liquid chromatography, enzyme-linked immunosorbent assay, capillary electrophoresis and fluorescence spectroscopy. However, these methods generally have the disadvantages of complex operation, expensive equipment and long detection period, lack of highly integrated design, and are difficult to realize automatic cleaning, real-time detection and online data transmission functions. Under the background of rapid development of analytical science, traditional detection technology has obvious limitations and is difficult to meet the actual needs of on-site rapid detection. SUMMARY

[0004] In view of this, the present application provides a device and method for real-time online monitoring of tetracycline, which can scan the photocurrent response of tetracycline at different wavelengths, construct a photoelectric fingerprint database of tetracycline and interferents, and reduce the misjudgment rate of enrofloxacin and other interferents through comparison and inspection, thereby meeting the actual needs of on-site rapid detection.

[0005] The technical scheme of the present application is as follows:

[0006] The utility model provides a kind of device and method for monitoring tetracycline in real time online, including box, rotating drum, driving mechanism, support plate, monochromator, quantitative liquid inlet mechanism and main control unit, relative support plate is provided in the box, reaction cylinder is provided in the middle of support plate, transparent window is provided in the side of reaction cylinder, its top and bottom are connected with sample tube and discharge valve respectively, the sample tube passes through box and extends outside, the inner wall of the box is equipped with mounting plate, the liquid pump is equipped on the mounting plate, the liquid pump is equipped on the sample tube, the discharge valve is connected with discharge pipe, the discharge pipe passes through box and extends outside, working electrode and counter electrode are equipped in the reaction cylinder, the side of working electrode is equipped with sensitive material layer, which is parallel with transparent window, rotating drum rotating sleeve is equipped outside reaction cylinder, and light transmission hole is uniformly arranged on the side of rotating drum around its axis, the driving mechanism is arranged on the top surface of reaction cylinder and is connected with rotating drum, the support plate is arranged on the inner wall of box and is located on the side of rotating drum, the monochromator is arranged on the side of support plate, the quantitative liquid inlet mechanism is arranged on the top surface of box and is communicated with reaction cylinder, the main control unit is arranged on the inner wall of box, and it includes extended gate transistor, current signal acquisition unit, analysis unit and storage unit, the gate of extended gate transistor is electrically connected with working motor, the drain of extended gate transistor is electrically connected with current signal acquisition unit, and the controller is electrically connected with storage unit, current signal acquisition unit, counter electrode, driving mechanism, monochromator, liquid pump and discharge valve.

[0007] Preferably, the driving mechanism includes a first motor, a gear and a gear ring, the first motor is arranged on the top surface of the reaction cylinder, the output shaft of the first motor is connected with the gear, the gear ring is arranged on the top surface of the rotating drum, and the gear is engaged with the gear ring.

[0008] Preferably, the quantitative liquid inlet mechanism includes a liquid inlet pipe, a quantitative pump, a measuring cylinder, a second motor, a rotating shaft, stirring blades and a feeding hopper, the measuring cylinder is arranged on the top surface of the box, the rotating shaft is rotatably arranged in the measuring cylinder, the second motor is arranged on the top surface of the measuring cylinder, the output shaft of the second motor penetrates through the measuring cylinder and is connected with the rotating shaft, the stirring blades are arranged on the side of the rotating shaft, the feeding hopper is arranged on the top surface of the measuring cylinder, the measuring cylinder is communicated with the top of the reaction cylinder through the liquid inlet pipe, and the quantitative pump is arranged on the liquid inlet pipe.

[0009] Preferably, the utility model further includes a human-computer interaction screen, the human-computer interaction screen is arranged on the side wall of the box, a wireless communication module is arranged on the human-computer interaction screen, and the main control unit is data-connected with the human-computer interaction screen.

[0010] Preferably, the sensitive material layer is g-C3N4 / TiO2 / CC (TCN) heterojunction electrode material.

[0011] Preferably, the utility model further includes a pH sensor and a conductivity sensor, the pH sensor and the conductivity sensor are arranged in the reaction cylinder and are respectively data-connected with the main control unit.

[0012] Preferably, the temperature sensor is arranged on the working electrode, the electric push rod is arranged on the top surface of the box body, the telescopic end of the electric push rod is connected to the heat filter, and the heat filter is located between the monochromator and the reaction cylinder.

[0013] Preferably, the fan is arranged on the side surface of the rotating cylinder.

[0014] Preferably, the cleaning mechanism comprises a third motor, a stirring rod and a cleaning brush, the plurality of stirring rods are arranged on the bottom of the reaction cylinder, the cleaning brush is arranged on the bottom surface of the stirring rod, and the output shaft of the third motor penetrates through the reaction cylinder and is connected to the stirring rod.

[0015] A detection method of a device for monitoring tetracycline in real time online comprises the following steps:

[0016] In step S1, the quantitative buffer and tetracycline are added into the reaction cylinder to form a tetracycline solution with a predetermined concentration.

[0017] In step S2, the monochromator is started to emit light rays with different wavelengths.

[0018] In step S3, the rotating cylinder is driven by the driving mechanism to rotate at a predetermined speed, so that the light rays are irradiated on the sensitive material layer at intervals.

[0019] In step S4, the drain current of the field effect transistor is detected by the current acquisition unit, and the detection result is transmitted to the analysis unit, and the photoelectric data of tetracycline are transmitted to the storage unit for storage.

[0020] In step S5, S1-S4 are repeated, and the photoelectric fingerprint data of tetracycline with different concentrations are collected and stored.

[0021] In step S6, the tetracycline is replaced by an interferent such as enrofloxacin, and the photoelectric fingerprint data of the interferent with different concentrations are collected and stored in the storage unit.

[0022] In step S7, the solution to be detected is pumped into the reaction cylinder by the liquid pump, and the photoelectric fingerprint data thereof are collected.

[0023] In step S8, the photoelectric fingerprint data of the solution to be detected and the fingerprint data of tetracycline are compared to confirm the initial concentration.

[0024] In step S9, the initial concentration data and the photoelectric fingerprint data of the interferent are verified, if the verification is passed, the final concentration is determined and output to the human-computer interaction screen, otherwise, the concentration is determined to be zero.

[0025] Compared with the prior art, the device has the following beneficial effects:

[0026] 1. The application provides a sensor detection device combining high sensitivity characteristics of field effect transistors and photoelectrochemical analysis technology. A monochromator is arranged, the monochromator emits light of a specific wavelength, and the photoelectric current response of tetracycline at different wavelengths can be scanned by using a rotating drum to generate photoelectric fingerprint data, a photoelectric fingerprint database is constructed, comparison and verification are carried out with the photoelectric current response fingerprints of interference substances such as enrofloxacin, real-time online monitoring of tetracycline solution is realized, and the misjudgment rate of enrofloxacin and other interference substances is reduced.

[0027] 2. The quantitative liquid feeding mechanism is arranged, a certain amount of tetracycline and buffer solution can be stirred and mixed uniformly, and quantitative addition can be carried out, so that the calibration of the detection device can be completed regularly, and the stability and detection precision of the detection device are maintained. DETAILED DESCRIPTION

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only preferred embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor.

[0029] Figure 1 It is a structural schematic diagram of the device for real-time online monitoring of tetracycline.

[0030] Figure 2 It is a structural schematic diagram of the device for real-time online monitoring of tetracycline. Figure 1 It is an enlarged view of A in FIG.

[0031] Figure 3 It is a sectional view of B-B in FIG. Figure 1 It is a sectional view of B-B in FIG.

[0032] Figure 4 It is a principle diagram of the device for real-time online monitoring of tetracycline.

[0033] Reference: 1, box; 2, support plate; 3, monochromator; 4, hot filter; 5, support plate; 6, first motor; 7, gear; 8, gear ring; 9, rotating drum; 10, light transmission hole; 11, transparent window; 12, sensitive material layer; 13, working electrode; 14, reaction cylinder; 15, stirring rod; 16, cleaning brush; 17, second motor; 18, quantitative pump; 19, measuring cylinder; 20, main control unit; 21, current signal acquisition unit; 22, extended gate transistor; 23, fan blade; 24, third motor; 25, rotating shaft; 26, stirring blade; 27, feed hopper; 28, sample inlet tube; 29, liquid pump; 30, mounting plate; 31, temperature sensor; 32, pH sensor; 33, conductivity sensor; 34, discharge valve; 35, discharge pipe; 36, electric push rod; 37, counter electrode; 38, human-computer interaction screen; 39, wireless communication module; 40, liquid inlet pipe; 41, analysis unit; 42, storage unit. DETAILED DESCRIPTION

[0034] In order to better understand the technical content of the present application, a specific embodiment is provided below, and the present application is further described in conjunction with the accompanying drawings.

[0035] Reference Figures 1 to 4The present invention provides a device for real-time online monitoring of tetracycline, comprising a box 1, a rotating drum 9, a driving mechanism, a support plate 2, a monochromator 3, a quantitative liquid inlet mechanism and a main control unit 20. Support plates 5 are relatively arranged in the box 1, a reaction cylinder 14 is arranged in the middle of the support plate 5, a transparent window 11 is provided on the side of the reaction cylinder 14, and an injection tube 28 and a discharge valve 34 are connected to the top and bottom thereof respectively. The injection tube 28 passes through the box 1 and extends to the outside. A mounting plate 30 is provided on the inner wall of the box 1, a liquid pump 29 is provided on the mounting plate 30, and the liquid pump 29 is provided on the injection tube 28. The discharge valve 34 is connected to the discharge pipe 35, and the discharge pipe 35 passes through the box 1 and extends to the outside. A working electrode 13 is provided in the reaction cylinder 14 to and the counter electrode 37, a sensitive material layer 12 is provided on the side of the working electrode 13, which is parallel to the transparent window 11, the rotating drum 9 is rotatably sleeved on the outside of the reaction drum 14, and light-transmitting holes 10 are evenly distributed on its side around its axis, the driving mechanism is provided on the top surface of the reaction drum 14 and is driven and connected to the rotating drum 9, the support plate 2 is provided on the inner wall of the box body 1 and is located on one side of the rotating drum 9, the monochromator 3 is provided on the side of the support plate 2, when the rotating drum 9 rotates, the light-transmitting holes 10 of the rotating drum 9 and the transparent window 11 of the reaction drum 14 are periodically aligned with the light path to achieve periodic illumination, and the working electrode 13 generates dark current due to the redox reaction of the solution when there is no light. This current is independent of the light response, and the pure photocurrent signal can be extracted through periodic illumination contrast, thereby suppressing low-frequency noise and improving the signal-to-noise ratio. The quantitative liquid inlet mechanism is provided on the top surface of the housing 1 and is connected to the reaction cylinder 14. The main control unit 20 is provided on the inner wall of the housing 1 and includes an extended gate transistor 22, a current signal acquisition unit 21, an analysis unit 41, and a storage unit 42. The gate of the extended gate transistor 22 is electrically connected to the working motor, and the current signal acquisition unit 21 is electrically connected to the drain of the extended gate transistor 22. The current signal acquisition unit 21 synchronously records the light current and dark current, and the analysis unit 41 calculates the difference between the light current and the dark current and generates a photoelectric fingerprint curve. The controller is electrically connected to the storage unit 42, the current signal acquisition unit 21, the counter electrode 37, the drive mechanism, the monochromator 3, the liquid pump 29, and the discharge valve 34.

[0036] Before the device performs detection work, first of all, the device is calibrated. Through the quantitative liquid inlet mechanism, the preset concentration tetracycline solution and other interference solution are filled into the reaction cylinder 14, and then the monochromator 3 and the driving mechanism are started. The monochromator 3 emits light of a preset wavelength, and the driving mechanism drives the rotating drum 9 to rotate. When the light transmission hole 10, the transparent window 11 and the monochromator 3 are on the horizontal line, the light passes through the light transmission hole 10 and the transparent window 11 and irradiates on the sensitive material layer 12 of the working electrode 13. The working electrode 13 undergoes a photocatalytic electrochemical reaction, and the gate generates an electromotive force. When the electromotive force reaches the gate valve threshold value, the drain and the source are connected. The current signal acquisition unit 21 collects the drain current signal, converts it into a digital signal and transmits it to the analysis unit 41. The analysis unit 41 generates photoelectric fingerprint data, which is a function of the drain current and a curve with the incident light wavelength as the independent variable, representing the photocatalytic electrochemical characteristics, and stores the photoelectric fingerprint data in the storage unit 42.

[0037] When the device works, first of all, the sample inlet tube 28 and the source to be detected are connected, and then the liquid pump 29 is started to draw the solution to be detected into the reaction cylinder 14. Then the monochromator 3 and the driving mechanism are started. The monochromator 3 emits light of a preset wavelength, and the driving mechanism drives the rotating drum 9 to rotate. When the light transmission hole 10, the transparent window 11 and the monochromator 3 are on the horizontal line, the light passes through the light transmission hole 10 and the transparent window 11 and irradiates on the sensitive material layer 12 of the working electrode 13. The working electrode 13 undergoes a photocatalytic electrochemical reaction, and the gate generates an electromotive force. When the electromotive force reaches the gate valve threshold value, the drain and the source are connected. The current signal acquisition unit 21 collects the drain current signal, converts it into a digital signal and transmits it to the analysis unit 41. The analysis unit 41 compares and analyzes the photoelectric fingerprint data and the photoelectric fingerprint data of tetracycline in the storage unit 42, generates initial concentration data, and then checks the initial concentration data with the photoelectric fingerprint data of enrofloxacin and other interference substances, so as to calculate the concentration value of tetracycline in the solution. After the detection is completed, the discharge valve 34 is started to discharge the solution from the reaction cylinder 14, and then the next detection is carried out, so as to realize the online monitoring function of the tetracycline solution concentration and reduce the misjudgment rate of enrofloxacin and other interference substances.

[0038] Preferably, the driving mechanism comprises a first motor 6, a gear 7 and a gear ring 8. The first motor 6 is arranged on the top surface of the reaction cylinder 14, and the output shaft thereof is connected to the gear 7. The gear ring 8 is arranged on the top surface of the rotating drum 9, and the gear 7 and the gear ring 8 are engaged with each other.

[0039] The driving mechanism is used to drive the rotating drum 9 to rotate. When the device works, the first motor 6 is started, which drives the gear 7 to rotate, and the gear 7 drives the gear ring 8 to rotate, thereby driving the rotating drum 9 to rotate around the axis of the reaction cylinder 14.

[0040] Preferably, the quantitative liquid feeding mechanism comprises a liquid feeding pipe 40, a quantitative pump 18, a measuring cylinder 19, a second motor 17, a rotating shaft 25, stirring blades 26 and a feeding hopper 27. The measuring cylinder 19 is arranged on the top surface of the box 1, the rotating shaft 25 is rotatably arranged in the measuring cylinder 19, the second motor 17 is arranged on the top surface of the measuring cylinder 19, the output shaft of the second motor 17 penetrates the measuring cylinder 19 and is connected to the rotating shaft 25, the stirring blades 26 are arranged on the side surface of the rotating shaft 25, the feeding hopper 27 is arranged on the top surface of the measuring cylinder 19, the measuring cylinder 19 is connected to the top of the reaction cylinder 14 through the liquid feeding pipe 40, and the quantitative pump 18 is arranged on the liquid feeding pipe 40.

[0041] The quantitative liquid feeding mechanism is used for quantitative extraction and delivery of the sample to be tested to the reaction cylinder 14 according to a preset volume, while ensuring uniform mixing. The measuring cylinder 19 is provided with scales, the operator adds buffer solution into the measuring cylinder 19 through the feeding hopper 27, a predetermined amount of sample or reagent is added into the measuring cylinder 19 when the buffer solution reaches a predetermined scale, the second motor 17 is started, the second motor 17 drives the rotating shaft 25 to rotate, thereby driving the stirring blades to stir the buffer solution, so that the sample and the buffer solution are uniformly stirred, and then the quantitative pump 18 is started to pump the solution into the reaction cylinder 14, so as to complete the calibration operation of the device.

[0042] Preferably, the device further comprises a human-computer interaction screen 38, the human-computer interaction screen 38 is arranged on the side wall of the box 1, the human-computer interaction screen 38 is provided with a wireless communication module 39, and the main control unit 20 is in data connection with the human-computer interaction screen 38.

[0043] When the quantitative liquid feeding mechanism fills tetracycline and different interferent solutions into the reaction cylinder 14, the operator can store the collected photoelectric fingerprint data into the storage unit 42 through the human-computer interaction screen 38, so as to call, compare and verify the tetracycline concentration when monitoring the tetracycline concentration; the operator can also clean the reaction cylinder 14 through the human-computer interaction screen 38, or set a time to fill clean water into the measuring cylinder 19, discharge the detection solution, and then fill clean water into the reaction cylinder 14 for flushing, so as to maintain the cleanliness of the reaction cylinder 14 and be beneficial to the stability of the detection precision; when the device is in real-time online detection of the solution, the human-computer interaction screen 38 displays the detected tetracycline concentration value in real time. The wireless communication module 39 is used for wireless data connection with other devices.

[0044] Preferably, the sensitive material layer 12 is a g-C3N4 / TiO2 / CC (TCN) heterojunction electrode material. The carbon cloth (CC) serves as a three-dimensional conductive substrate to provide mechanical support, enhance conductivity and provide mechanical stability. The g-C3N4 nanosheet is loaded on the surface of the TiO2 nanotube by a coating calcination method to form a close interface contact, the g-C3N4 / TiO2 forms a Z-type heterojunction to optimize the redox potential.

[0045] Preferably, pH sensor 32 and conductivity sensor 33 are arranged in reaction cylinder 14 and are in data connection with main control unit 20 respectively.

[0046] pH sensor 32 is used to detect the pH value of the solution to be measured; conductivity sensor 33 is used to detect the conductivity of the solution. The solution to be measured is stabilized at a predetermined pH value and conductivity.

[0047] Preferably, temperature sensor 31 is arranged on working electrode 13, electric push rod 36 is arranged on the top surface of box 1, the telescopic end of which is connected to heat filter 4, and heat filter 4 is located between monochromator 3 and reaction cylinder 14.

[0048] As the light continuously irradiates on sensitive material layer 12, the temperature of working electrode 13 will rise, and temperature sensor 31 detects the temperature of working electrode in real time. When the detected value is greater than the preset temperature value, electric push rod 36 is started, the telescopic end of electric push rod 36 is elongated to drive heat filter 4 to descend, heat filter 4 descends and is located between monochromator 3 and reaction cylinder 14, and the light emitted by monochromator 3 reduces the light energy after passing through heat filter 4, thereby reducing the temperature of working electrode 13, prolonging the working time of working electrode 13, and avoiding the influence of the detection precision caused by the excessively high temperature of working electrode.

[0049] Preferably, fan blade 23 is arranged on the side surface of rotating cylinder 9.

[0050] When the device performs the detection operation, the driving mechanism drives rotating cylinder 9 to rotate, so that fan blade 23 rotates differently, and the air flow generated can cool heat filter 4, which is beneficial to further prolong the working time of working electrode 13.

[0051] Preferably, cleaning mechanism is further included, which comprises third motor 24, stirring rod 15 and cleaning brush 16. A plurality of stirring rods 15 are arranged on the bottom of reaction cylinder 14, cleaning brush 16 is arranged on the bottom surface thereof, and third motor 24 is arranged on the bottom surface of reaction cylinder 14, the output shaft of which penetrates through reaction cylinder 14 and is connected to stirring rod 15.

[0052] Because tetracycline solutions are prone to scaling, after the device completes a test, the third motor 24 is activated. This rotation drives the stirring rod 15, which in turn drives the cleaning brush 16, which cleans the bottom of the reaction tube 14. Simultaneously, the discharge valve 34 is opened, allowing the solution to pass through the valve and be discharged from the discharge pipe 35. This prevents suspended matter or sediment from remaining at the bottom of the reaction tube 14, potentially affecting the next test. Furthermore, while the liquid pump 29 is pumping the test solution into the reaction tube 14, the third motor 24 is activated to rotate the stirring rod 15, stirring the solution evenly and improving the accuracy of tetracycline concentration testing.

[0053] A detection method for a device for real-time online monitoring of tetracycline comprises the following steps:

[0054] Step S1, adding a quantitative buffer solution and tetracycline into the reaction cylinder 14 to form a tetracycline solution of a predetermined concentration;

[0055] Step S2, starting the monochromator 3 to emit light of different wavelengths;

[0056] Step S3: Start the driving mechanism to drive the drum 9 to rotate at a preset speed so that the light is intermittently irradiated on the sensitive material layer 12;

[0057] Step S4: Start the current acquisition unit to detect the drain current of the field effect transistor 22 and transmit the detection result to the analysis unit 41. The analysis unit 41 transmits the photoelectric data of tetracycline to the storage unit 42 for storage;

[0058] Step S5, repeating S1-S4, collecting and storing photoelectric fingerprint data of tetracycline at different concentrations;

[0059] Step S6: replace tetracycline with an interfering substance such as enrofloxacin, collect photoelectric fingerprint data of interfering substances at different concentrations and store them in the storage unit 42;

[0060] Step S7: Start the liquid pump 29 to pump the solution to be tested into the reaction cylinder 14 and collect its photoelectric fingerprint data; Step S8: Compare the photoelectric fingerprint data with the tetracycline fingerprint data to confirm its initial concentration;

[0061] Step S9: Verify the initial concentration data and the photoelectric fingerprint data of the interference object. If the verification is passed, it is determined as the final concentration and output to the human-computer interaction screen 38; otherwise, the concentration is determined to be zero.

[0062] Before the monitoring device works, it needs to be detected and calibrated. Through the quantitative liquid feeding mechanism, the same concentration of tetracycline, enrofloxacin, ofloxacin, kanamycin and chloramphenicol and other commonly used antibiotics and glucose are added into the reaction cylinder 14 respectively, and the photoelectric fingerprint data thereof is collected and stored in the storage unit 42. During the detection process, the pH value is stable at 7.4, the conductivity is 120 μS / cm, and the rotating speed of the rotating cylinder 9 is 30 rpm. The definition of photoelectric fingerprint: I d =f(λ), that is, the drain current I d The response curve changes with the wavelength λ of incident light. The monochromator 3 scans the range of 300-600 nm with a step of 10 nm, and the I d of light / dark period is recorded at each wavelength point, and the pure photoelectric current is calculated:

[0063] ΔI=I 光 -I 暗

[0064] The standard curve ΔI-λ curve of tetracycline with different concentrations is stored.

[0065] The analysis unit 41 calculates the similarity of the measured curve and the standard tetracycline fingerprint:

[0066]

[0067] If the similarity is greater than 90%, the initial concentration is determined as the matching value.

[0068] Characteristic wavelength deviation check:

[0069]

[0070] If the deviation rate is greater than 30%, the interferent such as enrofloxacin is excluded, the check is passed, the final concentration is determined and output to the man-machine interaction screen 38; if the deviation rate is less than or equal to 30%, it is determined that it is an interferent, and the concentration of tetracycline is zero.

[0071] The above only describes the preferred embodiments of the present application and should not be used to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A device for real-time online monitoring of tetracycline, characterized in that: It includes a box body, a rotating drum, a driving mechanism, a support plate, a monochromator, a quantitative liquid inlet mechanism and a main control unit. Support plates are relatively arranged in the box body, a reaction cylinder is arranged in the middle of the support plates, a transparent window is provided on the side of the reaction cylinder, and an injection tube and a discharge valve are connected to the top and bottom thereof respectively. The injection tube passes through the box body and extends to the outside. A mounting plate is provided on the inner wall of the box body, a liquid pump is provided on the mounting plate, the liquid pump is arranged on the injection tube, the discharge valve is connected to the discharge tube, and the discharge tube passes through the box body and extends to the outside. A working electrode and a counter electrode are provided in the reaction cylinder, a sensitive material layer is provided on the side of the working electrode, which is parallel to the transparent window, and the rotating drum is rotatably sleeved on the outside of the reaction cylinder. , its side surfaces are evenly provided with light-transmitting holes around its axis, the driving mechanism is arranged on the top surface of the reaction cylinder and is driven and connected to the rotating cylinder, the support plate is arranged on the inner wall of the box and is located on one side of the rotating cylinder, the monochromator is arranged on the side of the support plate, the quantitative liquid inlet mechanism is arranged on the top surface of the box and is connected to the reaction cylinder, the main control unit is arranged on the inner wall of the box, and includes an extended gate transistor, a current signal acquisition unit, an analysis unit and a storage unit, the gate of the extended gate transistor is electrically connected to the working motor, the current signal acquisition unit is electrically connected to the drain of the extended gate transistor, and the controller is electrically connected to the storage unit, the current signal acquisition unit, the counter electrode, the driving mechanism, the monochromator, the liquid pump and the discharge valve.

2. A device for real-time online monitoring of tetracycline according to claim 1, characterized in that, The driving mechanism includes a first motor, a gear and a gear ring. The first motor is arranged on the top surface of the reaction cylinder, and its output shaft is driven by the gear. The gear ring is arranged on the top surface of the rotating cylinder, and the gear and the gear ring are meshed with each other.

3. A device for real-time online monitoring of tetracycline according to claim 1, characterized in that, The quantitative liquid feeding mechanism includes a liquid inlet pipe, a quantitative pump, a measuring cylinder, a second motor, a rotating shaft, a stirring blade and a feed hopper. The measuring cylinder is arranged on the top surface of the box body, and the rotating shaft is rotatably arranged in the measuring cylinder. The second motor is arranged on the top surface of the measuring cylinder, and its output shaft passes through the measuring cylinder and is driven to the rotating shaft. The stirring blade is arranged on the side of the rotating shaft, and the feed hopper is arranged on the top surface of the measuring cylinder. The measuring cylinder is connected to the top of the reaction cylinder through the liquid inlet pipe, and the quantitative pump is arranged on the liquid inlet pipe.

4. A device for real-time online monitoring of tetracycline according to claim 1, characterized in that, It also includes a human-computer interaction screen, which is arranged on the side wall of the box body. A wireless communication module is arranged on the human-computer interaction screen, and the main control unit is data-connected with the human-computer interaction screen.

5. The device for real-time online monitoring of tetracycline according to claim 1, characterized in that: The sensitive material layer is g-C3N4 / TiO2 / CC (TCN) heterojunction electrode material.

6. The device for real-time online monitoring of tetracycline according to claim 1, characterized in that: It also includes a pH sensor and a conductivity sensor, which are arranged in the reaction cylinder and are respectively connected to the main control unit for data transmission.

7. The device for real-time online monitoring of tetracycline according to claim 1, characterized in that: It also includes a temperature sensor, an electric push rod and a heat filter. The temperature sensor is arranged on the working electrode, the electric push rod is arranged on the top surface of the box body, and its telescopic end is connected to the heat filter. The heat filter is located between the monochromator and the reaction cylinder.

8. The device for real-time online monitoring of tetracycline according to claim 1, characterized in that: It also includes fan blades, which are arranged on the side of the rotating drum.

9. The device for real-time online monitoring of tetracycline according to claim 1, characterized in that: It also includes a cleaning mechanism, which includes a third motor, a stirring rod and a cleaning brush. The multiple stirring rods are rotatably arranged at the bottom of the reaction tube, the cleaning brush is arranged on the bottom surface thereof, and the third motor is arranged on the bottom surface of the reaction tube. The output shaft thereof passes through the reaction tube and is driven to connect to the stirring rod.

10. A detection method using the device for real-time online monitoring of tetracycline according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step S1, adding a quantitative buffer solution and tetracycline into a reaction cylinder to form a tetracycline solution of a predetermined concentration; Step S2, starting the monochromator to emit light of different wavelengths; Step S3: starting the driving mechanism to drive the drum to rotate at a preset speed so that the light is intermittently irradiated on the sensitive material layer; Step S4, starting the current acquisition unit to detect the drain current of the field effect transistor and transmitting the detection result to the analysis unit, and the analysis unit transmits the photoelectric data of tetracycline to the storage unit for storage; Step S5, repeating S1-S4, collecting and storing photoelectric fingerprint data of tetracycline at different concentrations; Step S6, replacing tetracycline with an interfering substance such as enrofloxacin, collecting photoelectric fingerprint data of interfering substances at different concentrations and storing them in a storage unit; Step S7: Start the liquid pump to pump the solution to be tested into the reaction cylinder and collect its photoelectric fingerprint data; Step S8: compare the photoelectric fingerprint data with the tetracycline fingerprint data to confirm the initial concentration; Step S9: Verify the initial concentration data and the photoelectric fingerprint data of the interference object. If the verification is passed, it is determined as the final concentration and output to the human-computer interaction screen; otherwise, the concentration is determined to be zero.

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