Method for obtaining a heavy ion track from a biological stack
By preprocessing the biological stacks and scanning them with laser confocal microscopy, the problems of large workload and cumbersome data acquisition in the heavy ion hit experiment of biological stacks were solved, and more accurate heavy ion track identification was achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- DALIAN MARITIME UNIVERSITY
- Filing Date
- 2025-07-16
- Publication Date
- 2026-04-17
AI Technical Summary
In existing technologies, heavy ion impact experiments conducted on biological stacks involve a huge workload, cumbersome data acquisition, and inaccurate track tracking due to inaccurate projection of biological materials.
The target biological stack was obtained by preprocessing the initial biological stack and scanning it in three dimensions. The stack was marked and attached based on the center position of the embryo. The actual projection of the embryo was cut out using a laser confocal microscope to identify the heavy ion track.
This improved data acquisition efficiency, avoided the time-sensitivity caused by the damage repair mechanism of biological materials, and ensured the accuracy of tracks and data.
Smart Images
Figure CN120802335B_ABST
Abstract
Description
Technical Field
[0001] This application belongs to the field of biomaterials technology, and in particular relates to a method for obtaining heavy ion tracks of embryos based on biological stacks. Background Technology
[0002] In spaceflight, the radiation environment is complex, and damage to organisms caused by high-energy heavy-ion impacts is a significant source of space radiation biological effects. To study the effects of heavy ions on organisms, capturing the trajectories of heavy ions is the first step. Biostacks are devices specifically designed for heavy-ion detection. By stacking biological materials with a solid-state nuclear track detector, heavy-ion tracks can be captured via the solid-state nuclear track and correlated with the corresponding organism.
[0003] In existing technologies, heavy ion impact experiments conducted by biological stacks suffer from extremely high workloads and cumbersome data acquisition. For example, heavy ion data acquisition for the Shijian-10 biological stack took nearly two years. With the advent of the space station era, the flight time and payload capacity of biological stacks have significantly increased. The accumulation of heavy ions on the probes has led to a 30-60 fold increase in heavy ion track counts within the same observation area. This increased payload capacity has significantly increased the workload of data acquisition. However, due to the damage repair mechanisms of organisms, biological materials exhibit significant time-sensitivity after reentry to Earth, and the duration of data acquisition directly affects the accuracy of the data.
[0004] Meanwhile, in determining the relative position and projected outline of rice embryos, existing techniques directly replace the planar projection of the embryo with an ellipse. However, the actual size and shape of the embryo are not ellipses with a major axis of 1.5 mm and a minor axis of 1 mm. Furthermore, when an optical microscope focuses on a fixed plane, it causes blurring at other imaging depths. Simply describing the outline of biological materials as an ellipse leads to significant errors during long-term flight, thus impacting subsequent research. Summary of the Invention
[0005] This application provides a method for obtaining heavy ion tracks of embryos based on biological stacking, which can solve the problem of inaccurate tracks caused by inaccurate data acquisition and inaccurate projection of biological materials in the prior art.
[0006] In a first aspect, embodiments of this application provide a method for obtaining heavy ion tracks of seed embryos based on biological stacking, including:
[0007] The biological stack obtained after the biological samples and detection materials are installed and returned to Earth via space flight is used as the initial biological stack. The initial biological stack is preprocessed to obtain the target biological stack. The six detection plate mounting surfaces of the target biological stack in three dimensions are scanned to obtain the initial scan image of the target biological stack.
[0008] The initial scan image is marked based on the center position of the embryo to obtain the target scan image;
[0009] The target scan image and the probe of the target biological stack are attached together to obtain an initial attachment. Then, a second mark is made on the non-contact surface of the initial attachment according to the center position of the embryo to obtain the target attachment.
[0010] The target bonding component is scanned using a laser confocal microscope to obtain an image of the embryo, and the actual projection of the embryo is cropped out at the center position of the embryo in the image based on a preset projection contour.
[0011] The heavy ion track of the embryo is identified based on the actual projection.
[0012] Optionally, the step of preprocessing the initial biological stack to obtain the target biological stack includes:
[0013] The initial probe within the initial biological stack is etched according to the preprocessing to obtain the target probe, and the target probe is fixed within the initial biological stack in the original position used to place the initial probe, thus obtaining the target biological stack.
[0014] Optionally, the step of marking the initial scan image based on the center position of the embryo to obtain the target scan image includes:
[0015] Using image processing software, the scanned image is first marked based on the center position of each embryo to obtain the target scanned image.
[0016] Optionally, the step of scanning the target bonding component using a laser confocal microscope to obtain an image of the embryo includes:
[0017] The target bonding component is scanned using a preset laser confocal microscope to obtain a spliced image of the embryo and a multi-field image composed of the spliced image by computer.
[0018] The sub-field-of-view map is transformed according to the attribute data of the stitched image to obtain the target sub-field-of-view map;
[0019] The target field-of-view images are manually stitched together to obtain an ultra-large field-of-view scan image of the location of the embryo, and the ultra-large field-of-view scan image is used as the image of the embryo.
[0020] Optionally, before the step of cropping the actual projection of the embryo at the center position of the embryo within the imaging image based on a preset projection contour, the method further includes:
[0021] Obtain the activity data and attribute data of the embryo;
[0022] Using 3D modeling software, the embryo is modeled based on the activity data and the attribute data to obtain an embryo model;
[0023] Based on the embryo model, a planar projection contour of the embryo is established, and the planar projection contour of the embryo is used as the preset projection contour.
[0024] Optionally, the step of obtaining the viability data and attribute data of the embryo includes:
[0025] The initial activity data of the embryos were obtained by tetrazolium staining, and the initial activity data were converted by water absorption and swelling ratio to obtain the activity data of the embryos.
[0026] The initial attribute data of the embryo were obtained based on the tetrazolium experiment. The initial attribute data included the length of the embryo, the width of the embryo, and the width of the embryo cross-sectional structure.
[0027] The volume data of the embryo during drying is determined based on the initial attribute data and the expansion coefficient of the embryo. The volume data, the length of the embryo, the width of the embryo, and the width of the embryo cross-sectional structure are collectively used as the attribute data.
[0028] Optionally, the step of modeling the embryo using 3D modeling software based on the activity data and the attribute data to obtain an embryo model includes:
[0029] Obtain the species information of the embryo, and determine the average size data of the embryo based on the species information;
[0030] Using 3D modeling software, the embryo is modeled based on the activity data, attribute data, and average size data to obtain an embryo model.
[0031] Optionally, the step of establishing the planar projection contour of the embryo based on the embryo model and using the planar projection contour of the embryo as the preset projection contour includes:
[0032] The initial planar projection contour of the embryo is established based on the embryo model;
[0033] The initial planar projection contour is processed according to preset requirements to obtain the planar projection contour, so that the planar projection contour is represented on the plane.
[0034] Optionally, the step of cropping the actual projection of the embryo at the center position of the embryo within the imaging image based on a preset projection contour includes:
[0035] The orientation data of the embryo is determined based on the imaging image;
[0036] Based on the directional data and the center position of the embryo within the imaging image, the actual projection of the embryo is cropped out on the preset projection contour.
[0037] Optionally, the laser confocal microscope is a laser confocal microscope equipped with a motorized stage.
[0038] The beneficial effects of the embodiments in this application compared with the prior art are:
[0039] The initial biological stack is preprocessed to obtain the target biological stack, and the target biological stack is scanned to obtain the initial scan image of the target biological stack. Based on the center position of the embryo, the initial scan image is marked for the first time to obtain the target scan image. The target scan image and the probe of the target biological stack are bonded together to obtain the initial bonding piece, and the non-contact surface of the initial bonding piece is marked for the second time according to the center position of the embryo to obtain the target bonding piece. The target bonding piece is scanned by a preset laser confocal microscope to obtain the image image of the embryo, and the actual projection of the embryo is cropped on a preset projection contour based on the image image and the center position of the embryo in the image image. The heavy ion track of the embryo is identified according to the actual projection. Compared to optical microscopes, using a pre-defined laser confocal microscope can improve the efficiency of data acquisition, enabling faster data collection and avoiding the time-consuming nature caused by the damage repair mechanisms of biological materials. By cropping the actual projection of the embryo from the pre-defined projection contour, the actual projection of the embryo is not represented by a fixed-size ellipse, making the projection of the embryo more accurate and thus the track determination results more accurate. Attached Figure Description
[0040] To more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0041] Figure 1This is a schematic flowchart of a method for obtaining heavy ion tracks of seed embryos based on biological stacking, provided in an embodiment of this application.
[0042] Figure 2 This is a flowchart illustrating a method for obtaining heavy ion tracks of seed embryos based on biological stacking, provided in another embodiment of this application.
[0043] Figure 3 This is a schematic diagram of the structure of a biological stack provided in an embodiment of this application. Detailed Implementation
[0044] In the following description, specific details such as particular system architectures and techniques are set forth for illustrative purposes and not for limitation, in order to provide a thorough understanding of the embodiments of this application. However, those skilled in the art will understand that this application may also be implemented in other embodiments without these specific details. In other instances, detailed descriptions of well-known systems, apparatuses, circuits, and methods have been omitted so as not to obscure the description of this application with unnecessary detail.
[0045] It should be understood that, when used in this application specification and the appended claims, the term "comprising" indicates the presence of the described features, integrals, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or a collection thereof.
[0046] It should also be understood that the term “and / or” as used in this application specification and the appended claims means any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.
[0047] As used in this application specification and the appended claims, the term "if" may be interpreted, depending on the context, as "when," "once," "in response to determination," or "in response to detection." Similarly, the phrase "if determined" or "if detected [the described condition or event]" may be interpreted, depending on the context, as meaning "once determined," "in response to determination," "once detected [the described condition or event]," or "in response to detection [the described condition or event]."
[0048] Furthermore, in the description of this application and the appended claims, the terms "first," "second," "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.
[0049] References to "one embodiment" or "some embodiments" as described in this specification mean that one or more embodiments of this application include a specific feature, structure, or characteristic described in connection with that embodiment. Therefore, the phrases "in one embodiment," "in some embodiments," "in other embodiments," "in still other embodiments," etc., appearing in different parts of this specification do not necessarily refer to the same embodiment, but rather mean "one or more, but not all, embodiments," unless otherwise specifically emphasized. The terms "comprising," "including," "having," and variations thereof mean "including but not limited to," unless otherwise specifically emphasized.
[0050] Figure 1 A schematic flowchart of the method for obtaining heavy ion tracks of embryos based on biological stacking provided in this application is shown. Embodiments of this application provide a method for obtaining heavy ion tracks of embryos based on biological stacking, including:
[0051] S101. The biological stack obtained after the biological samples and detection materials are installed and returned to Earth via space flight is used as the initial biological stack. The initial biological stack is preprocessed to obtain the target biological stack. The six detection plate mounting surfaces of the target biological stack in three dimensions are scanned to obtain the initial scan image of the target biological stack.
[0052] In one possible implementation, the step of preprocessing the initial bio-stacking to obtain the target bio-stacking includes:
[0053] The initial probe within the initial biological stack is etched according to the preprocessing to obtain the target probe, and the target probe is fixed within the initial biological stack in the original position used to place the initial probe, thus obtaining the target biological stack.
[0054] For example, the initial probe within the initial bio-stacking layer is etched using NaOH solution. After etching, the target probe is returned to its original position in the bio-stacking layer, and 3M tape is used to fix the four corners of the target probe (to prevent residue). The target probe is then scanned using an HP 4060 scanner with a resolution set to 4800 pixels, and saved as a TIFF image.
[0055] For example, such as Figure 3 As shown, the initial biological stack includes an embryo 2 and a solid nuclear track material 1.
[0056] S102. The initial scan image is marked for the first time based on the center position of the embryo to obtain the target scan image;
[0057] For example, the target scan image is obtained by first marking the scan image based on the center position of each embryo using image processing software, such as Photoshop.
[0058] S103. The target scan image and the probe of the target biological stack are attached to obtain an initial attachment, and a second mark is made on the non-contact surface of the initial attachment according to the center position of the embryo to obtain the target attachment.
[0059] For example, the size of the target scan image is adjusted to be the same as the size of the probe of the target biological stack, and the target scan image is printed in color. The target scan image and the probe of the target biological stack are then attached to obtain an initial attached piece. Using a marker or other marking pen, a second mark is made on the non-contact surface of the initial attached piece according to the center position of the embryo, to obtain the target attached piece.
[0060] S104. The target bonding component is scanned using a laser confocal microscope to obtain an image of the embryo, and the actual projection of the embryo is cut out at the center position of the embryo in the image based on a preset projection contour.
[0061] For example, a systematic scan was performed using a VK1000 laser confocal microscope or gold microscope equipped with a motorized stage. First, observation was performed using a 4x magnification lens, aligning the lens with the embryo's projection location. Then, the view was switched to 20x magnification. Using the mosaic measurement mode of the motorized stage, a scan range of x×y=4×5 fields of view was selected, resulting in an actual scan size of 2551×2375 μm. The embryo was ensured to be centered within the scan range. The focal plane was manually set, and the scan depth was set to 1.5 μm. The scan was then initiated.
[0062] In one possible implementation, the step of cropping the actual projection of the embryo at the center position of the embryo within the imaging image based on a preset projection contour includes:
[0063] The orientation data of the embryo is determined based on the imaging image;
[0064] Based on the directional data and the center position of the embryo within the imaging image, the actual projection of the embryo is cropped out on the preset projection contour.
[0065] For example, the orientation (orientation data) of the embryo is determined based on the display content of the image, and the actual projection of the embryo is cropped on a preset projection contour using the orientation (orientation data) and the center position of the embryo.
[0066] S105. Identify the heavy ion track of the embryo based on the actual projection.
[0067] The initial biological stack, consisting of biological samples and detection materials, is obtained after returning to Earth from space. This initial stack is preprocessed to obtain the target biological stack, and its initial scan image is obtained. The target biological stack is then scanned to obtain its initial scan image. Based on the center position of the embryo, the initial scan image is marked for the first time to obtain the target scan image. The target scan image and the detection sheet of the target biological stack are then bonded together to obtain an initial bond. Based on the center position of the embryo, a second mark is made on the non-contact surface of the initial bond to obtain the target bond. The target bond is scanned using a pre-set laser confocal microscope to obtain an image of the embryo. Based on the image and the center position of the embryo within the image, the actual projection of the embryo is cropped from a pre-set projection contour. The heavy ion track of the embryo is identified based on the actual projection. Compared to optical microscopes, using a pre-defined laser confocal microscope can improve the efficiency of data acquisition, enabling faster data collection and avoiding the time-consuming nature caused by the damage repair mechanisms of biological materials. By cropping the actual projection of the embryo from the pre-defined projection contour, the actual projection of the embryo is not represented by a fixed-size ellipse, making the projection of the embryo more accurate and thus the track determination results more accurate.
[0068] In one possible implementation, the step of scanning the target bonding component using a laser confocal microscope to obtain an image of the embryo includes:
[0069] The target bonding component is scanned using a preset laser confocal microscope to obtain a spliced image of the embryo and a multi-field image composed of the spliced image by computer.
[0070] The sub-field-of-view map is transformed according to the attribute data of the stitched image to obtain the target sub-field-of-view map;
[0071] The target field-of-view images are manually stitched together to obtain an ultra-large field-of-view scan image of the location of the embryo, and the ultra-large field-of-view scan image is used as the image of the embryo.
[0072] For example, when a computer stitches together segmented field-of-view images to form a composite image of an embryo, compression occurs during the stitching process, which leads to a decrease in clarity. Therefore, the segmented field-of-view images are converted according to the attribute data (pixels, size, etc.) of the composite image to obtain a target segmented field-of-view image. The embryo image is then obtained by manual stitching to ensure the quality of the image and facilitate subsequent track acquisition.
[0073] In one possible implementation, before the step of cropping the actual projection of the embryo at the center position of the embryo within the imaging image based on a preset projection contour, the method further includes:
[0074] Obtain the activity data and attribute data of the embryo;
[0075] Using 3D modeling software, the embryo is modeled based on the activity data and the attribute data to obtain an embryo model;
[0076] Based on the embryo model, a planar projection contour of the embryo is established, and the planar projection contour of the embryo is used as the preset projection contour.
[0077] For example, the step of obtaining the viability data and attribute data of the embryo includes:
[0078] The initial activity data of the embryos were obtained by tetrazolium staining, and the initial activity data were converted by water absorption and swelling ratio to obtain the activity data of the embryos.
[0079] The initial attribute data of the embryo were obtained based on the tetrazolium experiment. The initial attribute data included the length of the embryo, the width of the embryo, and the width of the embryo cross-sectional structure.
[0080] The volume data of the seed embryo during drying is determined based on the initial attribute data and the expansion coefficient of the seed embryo. The volume data, the length of the seed embryo, the width of the seed embryo, and the width of the cross-sectional structure of the seed embryo are collectively used as the attribute data.
[0081] Specifically, the size of the active center of the seed was determined using the tetrazolium staining method, and the water absorption and swelling ratio of the dried seeds was calculated. The experimental mechanism can be referred to the national standard. Based on the tetrazolium experiment, the width of the cross-sectional structure of the embryo, the length of the embryo, and the width of the embryo were obtained. The size of the embryo during drying was determined based on the measured swelling coefficient.
[0082] In one possible implementation, the step of modeling the embryo using 3D modeling software based on the activity data and the attribute data to obtain an embryo model includes:
[0083] Obtain the species information of the embryo, and determine the average size data of the embryo based on the species information;
[0084] Using 3D modeling software, the embryo is modeled based on the activity data, attribute data, and average size data to obtain an embryo model.
[0085] For example, since the average size of different types of embryos varies greatly, such as corn embryos and rice embryos, it is necessary to obtain the type information of the embryos and then determine the average size data of the embryos. Taking rice embryos as an example, the embryos are reconstructed in 3D using soildworks, and the average size of the rice embryos is assigned to the constructed 3D model.
[0086] In one possible implementation, the step of establishing a planar projection contour of the embryo based on the embryo model and using the planar projection contour of the embryo as the preset projection contour includes:
[0087] The initial planar projection contour of the embryo is established based on the embryo model;
[0088] The initial planar projection contour is processed according to preset requirements to obtain the planar projection contour, so that the planar projection contour is represented on the plane.
[0089] For example, a planar projection outline of a rice seed embryo is created and processed using Photoshop so that the size of the specified outline can be drawn on the plane.
[0090] In one possible embodiment, to better demonstrate the timeliness of this solution, the solution described in this application is compared with existing technologies using "person-hours" as the unit. This method can control the data acquisition time for a single CR-39 probe wafer to within 15 person-hours, and each probe wafer can collect approximately 3000 heavy ion tracks. Compared to the method used in the biological stacking of the Shijian-10 recoverable satellite, which processed 10 CR-39 wafers and collected fewer than 1000 data points (single heavy ion tracks), taking a total of 606 days, averaging approximately 60 days per wafer, with 3 personnel involved (approximately 1080 person-hours per wafer, with each person having 6 person-hours per day), averaging approximately 200 heavy ion tracks per wafer. Therefore, the processing speed of the entire probe wafer is increased by approximately 70 times, and the number of tracks detected per person-hour is increased by 37 times.
[0091] In one possible embodiment, such as Figure 2 As shown, the method for obtaining heavy ion tracks of seed embryos based on biological stacking described in this scheme includes:
[0092] After the bio-stacking was returned, the probe sheet (secured with silicone rubber to prevent loosening or detachment) was fixed in its original position using 3M tape. The interface was then scanned using a scanner. Since the actual size of the embryos was smaller than the markings on them, the bio-stacking scan was processed at a 1:1 scale to mark the center position of each embryo in the bio-stacking. The pattern was then printed, the probe sheet was attached to the printed pattern, and the center position of the embryo was marked on the non-contact surface using a marker. A laser confocal microscope was then used for scanning, ensuring that the scanned area completely aligns with the embryo's projection; the marker markings were only for positioning purposes.
[0093] This method uses a Keyence VK1000 laser confocal microscope with a compatible motorized stage. During measurement, the pre-marked probe slide is placed on the slide and centered on the stage. The laser confocal microscope scans the side of the probe slide that is in contact with the seed. Each probe slide is engraved with a number; the engraved side is not in contact with the seed. The microscope is adjusted via software to the standard 4x magnification mode. The embryo projection location is located in the field of view, and the magnification is then adjusted to 20x. The focus is adjusted to ensure a clear view. The light intensity is adjusted to avoid an overly bright or dark field of view. The measurement mode is then switched to a stitched measurement mode, and the automatic scanning function of the motorized stage generates a scanned field of view of the entire projection site. Set the scanning area to the entire projected area, adjust the scanning depth to 1.5 μm, and set the scanning plane to the focal plane. Then start the scan. After the scan is complete, two types of data will be generated: an image of the entire embryo exported by the stitching measurement function, and a segmented field-of-view image that forms this stitched image. The saved data format is VK file, which can only be opened using multi-file analysis software. The actual size of a rice embryo is 1.3 × 1.1 (mm), and generally, it is sufficient to collect samples from the surface of a 4 × 4 field-of-view probe.
[0094] After scanning, a surface image of the corresponding location will be obtained, which will then undergo further processing. First, the stitched pattern of the corresponding embryo will be opened using VK software. Due to the compression effect after image stitching, the clarity will decrease. The subfields will be manually superimposed according to the conversion ratio of the stitched pattern. Then, the embryo model built by SolidWorks will be used to project the outline of the plane. The specific projection of the embryo will be cropped according to the direction and center position of the embryo.
[0095] Those skilled in the art will recognize that the units and algorithm steps of the various examples described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are implemented in hardware or software depends on the specific application and design constraints of the technical solution. Those skilled in the art can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.
[0096] In the embodiments provided in this application, it should be understood that the disclosed apparatus / network device / terminal device and method can be implemented in other ways. For example, the apparatus / network device / terminal device embodiments described above are merely illustrative. For instance, the division of modules or units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be through some interfaces; the indirect coupling or communication connection between apparatuses or units may be electrical, mechanical, or other forms.
[0097] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.
[0098] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application, and should all be included within the protection scope of this application.
Claims
1. A method for obtaining a heavy ion track of a seed embryo based on bio-stacking, characterized in that, include: The biological stack obtained after the biological samples and detection materials are installed and returned to Earth via space flight is used as the initial biological stack. The initial biological stack is preprocessed to obtain the target biological stack. The six detection plate mounting surfaces of the target biological stack in three dimensions are scanned to obtain the initial scan image of the target biological stack. The initial scan image is marked based on the center position of the embryo to obtain the target scan image; The target scan image and the probe of the target biological stack are attached together to obtain an initial attachment. Then, a second mark is made on the non-contact surface of the initial attachment according to the center position of the embryo to obtain the target attachment. The target bonding component is scanned using a laser confocal microscope to obtain an image of the embryo, and the actual projection of the embryo is cropped out at the center position of the embryo in the image based on a preset projection contour. The heavy ion track of the embryo is identified based on the actual projection.
2. The method for obtaining heavy ion tracks of embryos based on biological stacking as described in claim 1, characterized in that, The step of preprocessing the initial biological stack to obtain the target biological stack includes: The initial probe within the initial biological stack is etched according to the preprocessing to obtain the target probe, and the target probe is fixed within the initial biological stack in the original position used to place the initial probe, thus obtaining the target biological stack.
3. The bio-stack based germband re-ion track acquisition method of claim 1, wherein, The step of marking the initial scan image based on the center position of the embryo to obtain the target scan image includes: Using image processing software, the scanned image is first marked based on the center position of each embryo to obtain the target scanned image.
4. The bio-stack based germband re-ion track acquisition method of claim 1, wherein, The step of scanning the target bonding component using a laser confocal microscope to obtain an image of the embryo includes: The target bonding component is scanned using a laser confocal microscope to obtain a spliced image of the embryo and a multi-field image composed of the spliced image by computer. The sub-field of view is transformed according to the attribute data of the stitched image to obtain the target sub-field of view; The target field-of-view images are manually stitched together to obtain an ultra-large field-of-view scan image of the location of the embryo, and the ultra-large field-of-view scan image is used as the image of the embryo.
5. The bio-stack based germband re-ion track acquisition method of claim 1, wherein, Before the step of cropping the actual projection of the embryo at the center position of the embryo in the imaging image based on the preset projection contour, the following operations need to be completed, including: Obtain the activity data and attribute data of the embryo; Using 3D modeling software, the embryo is modeled based on the activity data and the attribute data to obtain an embryo model; Based on the embryo model, a planar projection contour of the embryo is established, and the planar projection contour of the embryo is used as the preset projection contour.
6. The method for obtaining heavy ion tracks of embryos based on biological stacking as described in claim 5, characterized in that, The steps of obtaining the viability data and attribute data of the embryo include: The initial activity data of the embryos were obtained by tetrazolium staining, and the initial activity data were converted by water absorption and swelling ratio to obtain the activity data of the embryos. The initial attribute data of the embryo were obtained based on the tetrazolium experiment. The initial attribute data included the length of the embryo, the width of the embryo, and the width of the embryo cross-sectional structure. The volume data of the embryo during drying is determined based on the initial attribute data and the expansion coefficient of the embryo. The volume data, the length of the embryo, the width of the embryo, and the width of the embryo cross-sectional structure are collectively used as the attribute data.
7. The biomimetic-based germbreeding ion-track acquisition method according to claim 5, wherein, The step of modeling the embryo using 3D modeling software based on the activity data and the attribute data to obtain an embryo model includes: Obtain the species information of the embryo, and determine the average size data of the embryo based on the species information; Using 3D modeling software, the embryo is modeled based on the activity data, attribute data, and average size data to obtain an embryo model.
8. The bio-stack based germband re-ion track acquisition method of claim 5, wherein, The step of establishing the planar projection contour of the embryo based on the embryo model and using the planar projection contour of the embryo as the preset projection contour includes: The initial planar projection contour of the embryo is established based on the embryo model; The initial planar projection contour is processed according to preset requirements to obtain the planar projection contour, so that the planar projection contour is represented on the plane.
9. The bio-stack based germband re-ion track acquisition method of claim 1, wherein, The step of cropping the actual projection of the embryo at the center position of the embryo in the imaging image based on a preset projection contour includes: The orientation data of the embryo is determined based on the imaging image; Based on the directional data and the center position of the embryo within the imaging image, the actual projection of the embryo is cropped out on the preset projection contour.
10. The bio-stack based germbryo re- ion beam track acquisition method of claim 1, wherein, The laser confocal microscope is equipped with a motorized stage.
Citation Information
Patent Citations
Heavy ion track positioning method suitable for tiny seeds
CN120703811A
Method for 3D segmentation of a sample
EP4657395A1