Method and device for promoting heavy metal excretion in organisms using hydrogen nanobubble water

Abstract

The application relates to application of hydrogen nanobubble water in promoting heavy metal discharge in organisms, which comprises the following steps: placing an organism to be subjected to heavy metal discharge into a water tank, and introducing hydrogen nanobubble water into the water tank to promote heavy metal discharge in the organism. The application also relates to a device for promoting heavy metal discharge in organisms by using hydrogen nanobubble water and a discharge promoting method thereof. Hydrogen and oxygen are generated by electrolysis of ultrapure water, no byproduct is generated, no hardness and pH requirement is needed for water quality of the water tank, no resin filter is needed to remove impurities in the water, the upper limit of hydrogen production is high, the influence on the environment of the water tank is small, key water quality parameters such as hydrogen, dissolved oxygen, pH and ORP are adjusted by controlling the opening and shutdown time of the device, the heavy metal (cadmium, copper and hexavalent chromium) discharge in the organism is significantly promoted, no toxic side effect is generated, and the safety is good.

Description

Technical Field

[0001] This invention relates to the field of environmental protection, specifically to a method and apparatus for promoting the excretion of heavy metals from living organisms using hydrogen nanoparticle bubble water. Background Technology

[0002] In recent years, with the long-term discharge of industrial waste, agricultural non-point source pollution, and urban sewage, more and more heavy metal pollutants have entered water bodies through various pathways, polluting aquatic ecosystems. For example, nearshore fish can directly absorb large amounts of heavy metals from the marine environment or, as upper-level fish in the marine ecosystem's food chain, absorb heavy metals and accumulate them in their bodies. Heavy metal pollution also exists in aquaculture: firstly, heavy metals from the soil can enter water bodies through runoff and erosion; secondly, some feeds, drugs, and bottom sediment conditioners used in aquaculture also contain heavy metals, which are released into the water during use; and thirdly, wastewater and waste residue from surrounding industrial production contain heavy metals, easily polluting aquaculture water bodies. Heavy metals from different sources ultimately accumulate in water bodies, enriching in aquatic organisms and migrating through the food chain into the human body. In humans, heavy metals exceeding the body's tolerance level can interfere with normal physiological activities and threaten the health of people who consume aquatic products.

[0003] To alleviate oxidative stress, genetic problems, and other bodily damage caused by excessive heavy metal accumulation, the primary issue to address is inhibiting the accumulation of heavy metals in organisms. Currently, substances such as metal oxide nanoparticles, metallothioneins, and various organic detoxifiers have been put into practice. However, the drawbacks of these substances, such as high-dose side effects, low tolerability, and poor specificity, are also obvious, hindering their large-scale application.

[0004] Compared to traditional heavy metal detoxifiers, hydrogen molecules possess advantages such as selective antioxidant activity, strong diffusivity, and biocompatibility. Hydrogen nanobubble water, prepared using nanobubble technology, is a hydrogen-rich water characterized by high concentration, long shelf life, and strong antioxidant capacity, and has wide applications in medicine, environment, and agriculture. However, there are no reports on the use of hydrogen nanobubble water to promote the excretion of heavy metals from the body. Summary of the Invention

[0005] The purpose of this invention is to provide the application of hydrogen nanobubble water in promoting the excretion of heavy metals from living organisms.

[0006] In a preferred embodiment of the present invention, the application involves placing an organism to be excreted of heavy metals into a rearing tank, introducing hydrogen nanobubble water into the rearing tank, and using the hydrogen nanobubble water to promote the excretion of heavy metals from the organism.

[0007] In a preferred embodiment of the present invention, the water conditions for the rearing tank are as follows: tap water and deionized water are mixed at a volume ratio of 2-3:1, aerated for 24-32 h, and the oxidation-reduction potential (ORP) is controlled at 210-230 mV.

[0008] In a preferred embodiment of the present invention, the average particle size of the hydrogen nanobubble water is 400~600 nm.

[0009] In a preferred embodiment of the present invention, the number density of hydrogen nanobubbles in the water of the rearing tank is 1×10⁻⁶. 7 ~1×10 9 per mL.

[0010] In a preferred embodiment of the present invention, the hydrogen content in the water in the rearing tank is 0.5~0.9 mg / L.

[0011] In a preferred embodiment of the present invention, the dissolved oxygen content in the water of the rearing tank is 4~8 mg / L.

[0012] In a preferred embodiment of the present invention, the pH of the water in the rearing tank is 6-9.

[0013] In a preferred embodiment of the present invention, the feeding tank is equipped with an aeration device.

[0014] In a preferred embodiment of the present invention, the heavy metal includes any one or a combination of copper, cadmium, hexavalent chromium, zinc, aluminum, manganese, and silver.

[0015] In a preferred embodiment of the present invention, the organism to be expelled from heavy metals is any one of fish, shrimp, crab, or shellfish.

[0016] In a preferred embodiment of the present invention, the application can reduce the heavy metal content in the organism to which the heavy metal is to be excreted by more than 40%, preferably more than 50%, even more preferably more than 60%, and more preferably more than 70%.

[0017] Another object of the present invention is to provide a device for promoting the excretion of heavy metals from organisms using hydrogen nanoparticle bubble water, comprising: a proton exchange membrane electrolyzer 9, a high-pressure hydrogen mixing pump 4, a rearing tank 18, a booster pump 15, and a water tank 13.

[0018] The proton exchange membrane electrolyzer 9 includes a hydrogen outlet 7, an oxygen outlet 8, and an electrolyzer water inlet 10; the high-pressure hydrogen mixing pump 4 includes a hydrogen mixing pump water inlet 1, a hydrogen inlet 3, and a hydrogen mixing pump water outlet 6; the rearing tank 18 includes an aeration device 17, a rearing tank water inlet 19, and a rearing tank water outlet 20; the water tank 13 includes a water tank vent 11, a water tank air inlet 12, and a water tank water outlet 21; and the booster pump 15 includes a booster pump water inlet 14 and a booster pump water outlet 16.

[0019] The hydrogen outlet 7 is connected to the hydrogen inlet 3, and the oxygen outlet 8 is connected to the water tank inlet 12; the electrolytic cell inlet 10 is connected to the booster pump outlet 16; the hydrogen mixing pump outlet 6 is connected to the feeding tank inlet 19; the feeding tank outlet 20 is connected to the hydrogen mixing pump inlet 1; the aeration device 17 is connected to the water tank exhaust outlet 11; and the booster pump inlet 14 is connected to the water tank outlet 21.

[0020] In a preferred embodiment of the present invention, the high-pressure hydrogen mixing pump 4 further includes a flow valve 2 and a booster pipe 5.

[0021] In a preferred embodiment of the present invention, the power of the aeration device 17 is 20-25W.

[0022] In a preferred embodiment of the present invention, the water tank 13 contains ultrapure water.

[0023] In a preferred embodiment of the present invention, the working pressure of the booster pump 5 is 0.6~0.7 MPa.

[0024] In a preferred embodiment of the present invention, the average particle size of the hydrogen nanobubble water prepared by the device is 400~600 nm.

[0025] Another object of the present invention is to provide a method for promoting the excretion of heavy metals from the body using the device of the present invention that utilizes hydrogen nanobubble water to promote the excretion of heavy metals from the body, specifically including the following steps:

[0026] (1) Add the organisms to be excreted of heavy metals into the rearing tank 18, control the dissolved oxygen content in the water of the rearing tank 18 to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms.

[0027] (2) When the dissolved oxygen content in the water of the feeding tank 18 drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms.

[0028] (3) When the hydrogen content in the water of the feeding tank 18 drops below 0.1 mg / L, the device to promote the excretion of heavy metals from the organism is restarted.

[0029] (4) Repeat the shutdown and startup steps (2)-(3) for 24-48 hours.

[0030] In a preferred embodiment of the present invention, the specific steps for activating the device that promotes the excretion of heavy metals from the body are as follows:

[0031] s-1: Turn on the switch of the proton exchange membrane electrolyzer 9, and export the water stored in the water tank 13 through the booster pump 15 into the proton exchange membrane electrolyzer 9 to generate hydrogen and oxygen; at the same time, turn on the aeration device 17 in the breeding tank.

[0032] s-2: Hydrogen enters the high-pressure hydrogen mixing pump 4 through the hydrogen inlet 3. The water in the breeding tank enters the high-pressure hydrogen mixing pump 4 through the breeding tank outlet 20 and the hydrogen mixing pump inlet 1. The hydrogen mixes with the fish water and is pressurized by the pressurizing pipe 5 to obtain hydrogen nano bubble water.

[0033] s-3: Hydrogen nano bubble water enters the rearing tank 18 through the hydrogen mixing pump outlet 6 and the rearing tank inlet 19;

[0034] s-4: Oxygen enters the water tank 13 through the oxygen outlet 8, and then enters the breeding tank 18 through the water tank exhaust outlet 11.

[0035] In a preferred embodiment of the present invention, the specific steps for shutting down the device that promotes the excretion of heavy metals from the body are as follows: turning off the switch of the proton exchange membrane electrolyzer 9.

[0036] In a preferred embodiment of the present invention, during the stage when the device promoting the excretion of heavy metals from the organism is turned off, the aeration device 17 in the rearing tank 18 is always kept on.

[0037] In a preferred embodiment of the present invention, the power of the aeration device 17 is 20-25W.

[0038] In a preferred embodiment of the present invention, the water conditions for the rearing tank are as follows: tap water and deionized water are mixed at a volume ratio of 2-3:1, aerated for 24-32 h, and the oxidation-reduction potential (ORP) is controlled at 210-230 mV.

[0039] In a preferred embodiment of the present invention, during the operation of the device for promoting the excretion of heavy metals from the organism, the hydrogen content in the water in the rearing tank is 0.5~0.9 mg / L.

[0040] In a preferred embodiment of the present invention, during the operation of the device for promoting the excretion of heavy metals from the organism, the dissolved oxygen content in the water in the rearing tank is 4-8 mg / L.

[0041] In a preferred embodiment of the present invention, during the activation of the device for promoting the excretion of heavy metals from the organism, the number density of hydrogen nanobubbles in the water of the rearing tank is 1×10⁻⁶. 7 ~1×10 9 per mL.

[0042] In a preferred embodiment of the present invention, during the activation of the device for promoting the excretion of heavy metals from the organism, the pH of the water in the rearing tank is 6-9.

[0043] In a preferred embodiment of the present invention, the average particle size of the hydrogen nanobubble water is 400~600 nm.

[0044] In a preferred embodiment of the present invention, the water tank 13 contains ultrapure water.

[0045] In a preferred embodiment of the present invention, the working pressure of the booster pump 5 is 0.6~0.7 MPa.

[0046] In a preferred embodiment of the present invention, the heavy metal includes any one or a combination of copper, cadmium, hexavalent chromium, zinc, aluminum, manganese, and silver.

[0047] In a preferred embodiment of the present invention, the organism to be expelled from heavy metals is any one of fish, shrimp, crab, or shellfish.

[0048] In a preferred embodiment of the present invention, the application can reduce the heavy metal content in the organism to which the heavy metal is to be excreted by more than 40%, preferably more than 50%, even more preferably more than 60%, and more preferably more than 70%.

[0049] Unless otherwise stated, when this invention relates to percentages between liquids, the percentage is volume / volume percentage; when this invention relates to percentages between liquids and solids, the percentage is volume / weight percentage; when this invention relates to percentages between solids and liquids, the percentage is weight / volume percentage; the remainder is weight / weight percentage.

[0050] The present invention employs the following detection method:

[0051] (1) Dissolved oxygen in water is determined using the oxygen electrode method: Prepare 300 mL of 5% sodium sulfite solution by weight, immerse the probe of the online dissolved oxygen meter completely in the solution, use the zeroing function for calibration, and then rinse the probe 2-3 times with ultrapure water before inserting it into the solution to be tested for online monitoring. When using the device, the probe should not touch the container wall. When changing the test solution, the probe should be rinsed 2-3 times with ultrapure water.

[0052] (2) The determination of hydrogen content in water was made in accordance with the "Methods for determination of gas content in microbubble dispersion systems in water - Part 2: Hydrogen content" (ISO 7383-2:2024).

[0053] (3) Detection of the number density of nano-sized bubbles: Use 2 mL of ultrapure water to rinse the sample cell. When no obvious bubbles are observed on the instrument operation interface, use a syringe to push 2 mL of sample into the injection port. During the instrument analysis, the distribution of the number density of micro-nano-sized bubbles in hydrogen nano-bubble water is obtained by utilizing light scattering and the Brownian motion of nanoparticles.

[0054] (4) The heavy metal content in the fish to be digested was detected by ICP-MS: The frozen zebrafish samples were taken out, thawed, and the surface moisture was absorbed with filter paper. The weight of each zebrafish was then weighed using a high-precision electronic balance. After weighing and recording, the sample to be digested was placed in a 10 mL digestion tube, and 2 mL of 68% concentrated nitric acid was added to each tube. The working temperature of the digester was set to 120 ℃. After heating to 120 ℃, the timer was started. After digestion for two hours, the instrument was turned off. After complete cooling, the digestion tube was removed, and a clear light yellow solution was obtained. The solution was calibrated to the 10 mL mark of the digestion tube with ultrapure water, and the sample was diluted 5 times. Then, all 10 mL of liquid was transferred to a 50 mL centrifuge tube, and 20 mL of ultrapure water was added to each centrifuge tube. The sample was diluted a total of 15 times, and the acid concentration met the requirements for ICP-MS operation. Impurities were filtered using a 0.45 μm filter membrane and a 10 mL syringe to meet the standards required for ICP-MS detection.

[0055] Wash a 500 mL polyethylene bottle, add 10 mL of 68% concentrated nitric acid, then add 330 mL of ultrapure water, shake well, and prepare a 2% dilute nitric acid solution. Use this solution to prepare a 10 μg / L In internal standard solution and heavy metal standard solutions:

[0056] Copper standard solutions: concentration gradients of 1 μg / L, 2 μg / L, 4 μg / L, 8 μg / L, 16 μg / L, and 20 μg / L;

[0057] Cadmium standard solutions: concentration gradients of 1 μg / L, 2 μg / L, 4 μg / L, 8 μg / L, 16 μg / L, and 20 μg / L;

[0058] Standard solutions of hexavalent chromium: concentration gradients of 5 μg / L, 10 μg / L, 15 μg / L, 20 μg / L, 40 μg / L, and 80 μg / L.

[0059] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0060] (1) This invention is the first to discover that hydrogen nanobubble water can promote the excretion of heavy metals in organisms. By controlling the stable hydrogen content and water quality indicators (dissolved oxygen, pH, ORP, etc.) suitable for the survival of organisms, it has a significant promoting effect on the excretion of heavy metals (cadmium, copper, hexavalent chromium) in organisms. At the same time, it has no toxic side effects on the organisms themselves and has good safety.

[0061] (2) The device of the present invention promotes the excretion of heavy metals from organisms by using ultrapure water electrolysis to generate hydrogen and oxygen, without the generation of byproducts. It has no requirements on the hardness and pH of the water in the breeding tank, and does not require water quality adjustment or resin filters to remove impurities from the water. At the same time, the proton electrolysis cell and the breeding tank are completely separated, so there is no interference with the tank environment. The equipment is simple and easy to operate, and is suitable for use in farms and ordinary households.

[0062] (3) The present invention uses a device to promote the excretion of heavy metals in organisms to promote the excretion of heavy metals. It has a high upper limit of hydrogen production and has little impact on the environment in the breeding tank. By controlling the opening and closing time of the device, key water quality parameters such as hydrogen, dissolved oxygen, pH, and ORP can be adjusted to increase hydrogen mass transfer efficiency and achieve stable hydrogen content and water quality indicators suitable for the survival of organisms. It has a significant promoting effect on the excretion of heavy metals (cadmium, copper, hexavalent chromium) in organisms, and the excretion time is short and the safety is high. Attached Figure Description

[0063] Figure 1 This invention provides a schematic diagram of a device for promoting the excretion of heavy metals from organisms using hydrogen nanoparticle bubble water. The device includes: 1—hydrogen mixing pump inlet; 2—flow valve; 3—hydrogen inlet; 4—high-pressure hydrogen mixing pump; 5—boost pipe; 6—hydrogen mixing pump outlet; 7—hydrogen outlet; 8—oxygen outlet; 9—proton exchange membrane electrolyzer; 10—electrolyzer inlet; 11—water tank vent; 12—water tank air inlet; 13—ultrapure water tank; 14—boost pump inlet; 15—boost pump; 16—boost pump outlet; 17—aeration device; 18—feeding tank; 19—feeding tank inlet; 20—feeding tank outlet; 21—water tank outlet.

[0064] Figure 2. Study on water quality parameters in the feeding tank during the startup process of the device for promoting the excretion of heavy metals from organisms using hydrogen nanobubble water according to the present invention.

[0065] Figure 3 This invention relates to a device that utilizes hydrogen nanobubble water to promote the excretion of heavy metals from living organisms, and to the study of the excretion of copper from living organisms.

[0066] Figure 4 This invention relates to a device that utilizes hydrogen nanobubble water to promote the excretion of heavy metals from living organisms, and to the study of the excretion of cadmium from living organisms.

[0067] Figure 5 This invention relates to a device that utilizes hydrogen nanobubble water to promote the excretion of heavy metals from organisms, and to the study of the excretion of chromium from organisms. Detailed Implementation

[0068] The embodiments of the present invention will be described in detail below with reference to examples. However, these examples are only used to illustrate and explain the present invention and are not intended to limit the present invention.

[0069] The gas chromatograph (SHIMADZU, Japan, model GC-2010), dissolved oxygen online monitor (QCONTUMS, USA, model QC7687), ORP online monitor (QCONTUMS, USA, model QC3580), nanoparticle tracking and analysis system (Particle Metrix, Germany, model ZetaView), and proton exchange membrane electrolyzer (Guangdong Hydrogen Source New Energy, China, model CF200ML) used in the examples.

[0070] Example 1: This invention relates to a device that utilizes hydrogen nanobubble water to promote the excretion of heavy metals from living organisms.

[0071] A schematic diagram of the device for promoting the excretion of heavy metals from the body according to this invention is shown below. Figure 1 The system includes: a proton exchange membrane electrolyzer 9, a high-pressure hydrogen mixing pump 4, a rearing tank 18, a booster pump 15, and a water tank 13. The proton exchange membrane electrolyzer 9 includes a hydrogen outlet 7, an oxygen outlet 8, and an electrolyzer inlet 10. The high-pressure hydrogen mixing pump 4 includes a hydrogen mixing pump inlet 1, a hydrogen inlet 3, and a hydrogen mixing pump outlet 6. The rearing tank 18 includes an aeration device 17, a rearing tank inlet 19, and a rearing tank outlet 20. The water tank 13 includes a water tank vent 11, a water tank air inlet 12, and a water tank outlet 21. The booster pump 15 includes a booster pump inlet 14 and a booster pump outlet 16.

[0072] The hydrogen outlet 7 is connected to the hydrogen inlet 3, and the oxygen outlet 8 is connected to the water tank inlet 12; the electrolytic cell inlet 10 is connected to the booster pump outlet 16; the hydrogen mixing pump outlet 6 is connected to the feeding tank inlet 19; the feeding tank outlet 20 is connected to the hydrogen mixing pump inlet 1; the aeration device 17 is connected to the water tank exhaust outlet 11; and the booster pump inlet 14 is connected to the water tank outlet 21.

[0073] The specific steps for activating the device that promotes the excretion of heavy metals from the body are as follows:

[0074] s-1: Turn on the switch of the proton exchange membrane electrolyzer 9, and export the water stored in the water tank 13 through the booster pump 15 into the proton exchange membrane electrolyzer 9 to generate hydrogen and oxygen; at the same time, turn on the aeration device 17 in the breeding tank.

[0075] s-2: After hydrogen enters the high-pressure hydrogen mixing pump 4 through the hydrogen inlet 3, the fish water in the breeding tank enters the high-pressure hydrogen mixing pump 4 through the breeding tank outlet 20 and the hydrogen mixing pump inlet 1. The hydrogen mixes with the water in the breeding tank and is pressurized by the booster pipe 5 to obtain hydrogen nano bubble water.

[0076] s-3: Hydrogen nano bubble water enters the rearing tank 18 through the hydrogen mixing pump outlet 6 and the rearing tank inlet 19;

[0077] s-4: Oxygen enters the water tank 13 through the oxygen outlet 8, and then enters the breeding tank 18 through the water tank exhaust outlet 11.

[0078] The specific steps for shutting down the device that promotes the excretion of heavy metals from the body are as follows: turn off the switch of the proton exchange membrane electrolyzer 9.

[0079] During the phase where the device promoting the excretion of heavy metals from the organism is turned off, the aeration device 17 in the rearing tank 18 is always kept on.

[0080] The water conditions for the breeding tank are as follows: mix tap water and deionized water at a volume ratio of 2-3:1, aerate for 24-32 h, and control the oxidation-reduction potential (ORP) at 210-230 mV.

[0081] The aeration device 17 has a power of 6-10W. It maintains the dissolved oxygen content in the breeding tank at a range suitable for the survival of organisms by using oxygen generated by electrolysis in the electrolytic cell.

[0082] The device produces hydrogen nanobubble water with an average particle size of 400~600 nm. By controlling the number density of hydrogen nanobubbles introduced into the water tank, the hydrogen mass transfer efficiency is increased, and the hydrogen content in the water tank is controlled.

[0083] The water in the breeding tank has a pH of 6-9, and the water in the water tank 13 is ultrapure water.

[0084] The working pressure of the booster pump 5 is 0.6~0.7 MPa.

[0085] Experimental Example 1 This invention relates to a device that utilizes hydrogen nanoparticle water to promote the excretion of heavy metals from living organisms, and its application in the study of copper excretion from living organisms.

[0086] Prepare fish tank water: Use a 100 L plastic bucket, with a volume ratio of tap water to deionized water of approximately 3:1. Aerate for 24 hours until the ORP is around 230 mV.

[0087] Preparation of copper exposure solution: Add 3 μL of 1000 mg / L copper ion standard solution (the standard solution was purchased from the National Institute of Metrology) to 5L of dechlorinated tap water and stir well.

[0088] 1. Laboratory animals

[0089] Thirty zebrafish, ranging in length from 1.8 to 2.5 cm, were selected and cultured in 30 L of aquarium water at 25 °C. They were fed brine shrimp larvae once daily, and their condition was regularly checked, with excrement and dead fish removed promptly. Water level changes were monitored, and water was replenished as needed to account for evaporation. Feeding was stopped 24 hours prior to the experiment.

[0090] 2. Experimental Methods

[0091] Thirty zebrafish were added to a copper exposure solution and exposed for 8 hours. At 0, 2, 4 and 8 hours, three fish were quickly removed from the exposure solution and aliquoted into three numbered 5 mL flip-top centrifuge tubes. These tubes were then frozen and stored as the accumulation group. The heavy metal content accumulated in the fish of the accumulation group was determined by ICP-MS.

[0092] The remaining 18 zebrafish after exposure were quickly rinsed 2-3 times with deionized water and then added to the rearing tank of the apparatus in Example 1 containing 30L of fish tank water for a 24-hour excretion-inducing experiment. The excretion-inducing experiment conditions were as follows:

[0093] (1) Control the dissolved oxygen content in the water of the feeding tank to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms;

[0094] (2) When the dissolved oxygen content in the water in the feeding tank drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms.

[0095] (3) When the hydrogen content in the water of the feeding tank drops below 0.1 mg / L, restart the device to promote the excretion of heavy metals from the organisms.

[0096] (4) Repeat the shutdown and startup steps (2)-(3) for 24 hours.

[0097] The specific steps for activating the device that promotes the excretion of heavy metals from the body are as follows:

[0098] s-1: Turn on the switch of the proton exchange membrane electrolyzer 9, and export the water stored in the water tank 13 through the booster pump 15 into the proton exchange membrane electrolyzer 9 to generate hydrogen and oxygen; at the same time, turn on the aeration device 17 in the breeding tank.

[0099] s-2: Hydrogen enters the high-pressure hydrogen mixing pump 4 through the hydrogen inlet 3. The water in the breeding tank enters the high-pressure hydrogen mixing pump 4 through the breeding tank outlet 20 and the hydrogen mixing pump inlet 1. The hydrogen mixes with the fish water and is pressurized by the pressurizing pipe 5 to obtain hydrogen nano bubble water.

[0100] s-3: Hydrogen nano bubble water enters the rearing tank 18 through the hydrogen mixing pump outlet 6 and the rearing tank inlet 19;

[0101] s-4: Oxygen enters the water tank 13 through the oxygen outlet 8, and then enters the breeding tank 18 through the water tank exhaust outlet 11.

[0102] The specific steps for shutting down the device that promotes the excretion of heavy metals from the body are as follows: turn off the switch of the proton exchange membrane electrolyzer 9.

[0103] Throughout the entire process of promoting excretion, the aeration device 17 in the rearing tank 18 remains on.

[0104] Three fish were quickly removed from the tank at 3h, 8h, 13h, 16h, 20h and 24h of the ovulation induction experiment, and divided into three numbered 5 mL flip-top centrifuge tubes. The tubes were then frozen and stored, and the copper content in the fish was determined by ICP-MS.

[0105] 3. Results and Discussion

[0106] from Figure 2a , Figure 2b , Figure 2c It can be seen that the hydrogen content in the water of the rearing tank remained stable at 0.4~0.9 mg / L, the dissolved oxygen content remained stable at 4.5~8 mg / L, and the nanobubble number density remained stable at 1.2×10⁻⁶. 7 -1.5×10 7 The pH of the water is 6-9.

[0107] from Figure 3 The results show that during the 8-hour accumulation phase, the amount of copper accumulated was significantly positively correlated with time. Compared to the 8-hour accumulation phase, the 24-hour ovulation induction group showed a significant decrease in copper content in the fish.

[0108] Experimental Example 2 This invention relates to a device that utilizes hydrogen nanoparticle bubble water to promote the excretion of heavy metals from organisms, and is used in the study of cadmium excretion from organisms.

[0109] Prepare fish tank water: Use a 100 L plastic bucket, with a volume ratio of tap water to deionized water of approximately 3:1. Aerate for 24 hours until the ORP is around 230 mV.

[0110] To prepare an 800 μg / L cadmium exposure solution: Add 4 μL of 1000 mg / L cadmium ion standard solution (the standard solution was purchased from the National Institute of Metrology) to 5L of dechlorinated tap water and stir well.

[0111] 1. Laboratory animals

[0112] Thirty zebrafish, ranging in length from 1.8 to 2.5 cm, were selected and cultured in 30 L of aquarium water at 25 °C. They were fed brine shrimp larvae once daily, and their condition was regularly checked, with excrement and dead fish removed promptly. Water level changes were monitored, and water was replenished as needed to account for evaporation. Feeding was stopped 24 hours prior to the experiment.

[0113] 2. Experimental Methods

[0114] Thirty zebrafish were added to the cadmium exposure solution and exposed for 8 hours. At 0h, 2h, 4h and 8h, three fish were quickly taken from the exposure solution and divided into three numbered 5 mL flip-top centrifuge tubes. These tubes were then frozen and stored as the accumulation group. The heavy metal content accumulated in the fish of the accumulation group was determined by ICP-MS.

[0115] The remaining 18 zebrafish after exposure were quickly rinsed 2-3 times with deionized water and then added to the rearing tank of the apparatus in Example 1 containing 30L of fish tank water for a 24-hour excretion-inducing experiment. The excretion-inducing experiment conditions were as follows:

[0116] (1) Control the dissolved oxygen content in the water of the feeding tank to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms;

[0117] (2) When the dissolved oxygen content in the water in the feeding tank drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms.

[0118] (3) When the hydrogen content in the water of the feeding tank drops below 0.1 mg / L, restart the device to promote the excretion of heavy metals from the organisms.

[0119] (4) Repeat the shutdown and startup steps (2)-(3) for 24 hours.

[0120] The specific steps for activating the device that promotes the excretion of heavy metals from the body are as follows:

[0121] s-1: Turn on the switch of the proton exchange membrane electrolyzer 9, and export the water stored in the water tank 13 through the booster pump 15 into the proton exchange membrane electrolyzer 9 to generate hydrogen and oxygen; at the same time, turn on the aeration device 17 in the breeding tank.

[0122] s-2: Hydrogen enters the high-pressure hydrogen mixing pump 4 through the hydrogen inlet 3. The water in the breeding tank enters the high-pressure hydrogen mixing pump 4 through the breeding tank outlet 20 and the hydrogen mixing pump inlet 1. The hydrogen mixes with the fish water and is pressurized by the pressurizing pipe 5 to obtain hydrogen nano bubble water.

[0123] s-3: Hydrogen nano bubble water enters the rearing tank 18 through the hydrogen mixing pump outlet 6 and the rearing tank inlet 19;

[0124] s-4: Oxygen enters the water tank 13 through the oxygen outlet 8, and then enters the breeding tank 18 through the water tank exhaust outlet 11.

[0125] The specific steps for shutting down the device that promotes the excretion of heavy metals from the body are as follows: turn off the switch of the proton exchange membrane electrolyzer 9.

[0126] Throughout the entire process of promoting excretion, the aeration device 17 in the rearing tank 18 remains on.

[0127] Three fish were quickly removed from the tank at 3h, 8h, 13h, 16h, 20h and 24h of the ovulation induction experiment, and divided into three numbered 5 mL flip-top centrifuge tubes. The tubes were then frozen and stored, and the cadmium content in the fish was determined by ICP-MS.

[0128] 3. Results and Discussion

[0129] from Figure 4 The results show that during the 8-hour accumulation phase, the amount of cadmium accumulated was significantly positively correlated with time. Compared to the 8-hour accumulation phase, the cadmium content in the fish was significantly reduced in the 24-hour induction phase.

[0130] Experimental Example 3 This invention relates to a device that utilizes hydrogen nanoparticle water to promote the excretion of heavy metals from living organisms, and its application in the study of hexavalent chromium excretion from living organisms.

[0131] Prepare fish tank water: Use a 100 L plastic bucket, with a volume ratio of tap water to deionized water of approximately 3:1. Aerate for 24 hours until the ORP is around 230 mV.

[0132] To prepare an 800 μg / L chromium exposure solution: Add 40 μL of 100 mg / L hexavalent chromium ion standard solution (the standard solution was purchased from the National Institute of Metrology) to 5L of dechlorinated tap water and stir well.

[0133] 1. Laboratory animals

[0134] Thirty zebrafish, ranging in length from 1.8 to 2.5 cm, were selected and cultured in 30 L of aquarium water at 25 °C. They were fed brine shrimp larvae once daily, and their condition was regularly checked, with excrement and dead fish removed promptly. Water level changes were monitored, and water was replenished as needed to account for evaporation. Feeding was stopped 24 hours prior to the experiment.

[0135] 2. Experimental Methods

[0136] Thirty zebrafish were added to the chromium exposure solution and exposed for 8 hours. At 0, 2, 4 and 8 hours, three fish were quickly taken from the exposure solution and divided into three numbered 5 mL flip-top centrifuge tubes. These tubes were then frozen and stored as the accumulation group. The heavy metal content accumulated in the fish of the accumulation group was determined by ICP-MS.

[0137] The remaining 18 zebrafish after exposure were quickly rinsed 2-3 times with deionized water and then added to the rearing tank of the apparatus in Example 1 containing 30L of fish tank water for a 24-hour excretion-inducing experiment. The excretion-inducing experiment conditions were as follows:

[0138] (1) Control the dissolved oxygen content in the water of the feeding tank to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms;

[0139] (2) When the dissolved oxygen content in the water in the feeding tank drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms.

[0140] (3) When the hydrogen content in the water of the feeding tank drops below 0.1 mg / L, restart the device to promote the excretion of heavy metals from the organisms.

[0141] (4) Repeat the shutdown and startup steps (2)-(3) for 24 hours.

[0142] The specific steps for activating the device that promotes the excretion of heavy metals from the body are as follows:

[0143] s-1: Turn on the switch of the proton exchange membrane electrolyzer 9, and export the water stored in the water tank 13 through the booster pump 15 into the proton exchange membrane electrolyzer 9 to generate hydrogen and oxygen; at the same time, turn on the aeration device 17 in the breeding tank.

[0144] s-2: Hydrogen enters the high-pressure hydrogen mixing pump 4 through the hydrogen inlet 3. The water in the breeding tank enters the high-pressure hydrogen mixing pump 4 through the breeding tank outlet 20 and the hydrogen mixing pump inlet 1. The hydrogen mixes with the fish water and is pressurized by the pressurizing pipe 5 to obtain hydrogen nano bubble water.

[0145] s-3: Hydrogen nano bubble water enters the rearing tank 18 through the hydrogen mixing pump outlet 6 and the rearing tank inlet 19;

[0146] s-4: Oxygen enters the water tank 13 through the oxygen outlet 8, and then enters the breeding tank 18 through the water tank exhaust outlet 11.

[0147] The specific steps for shutting down the device that promotes the excretion of heavy metals from the body are as follows: turn off the switch of the proton exchange membrane electrolyzer 9.

[0148] Throughout the entire process of promoting excretion, the aeration device 17 in the rearing tank 18 remains on.

[0149] Three fish were quickly removed from the tank at 3h, 8h, 13h, 16h, 20h and 24h of the ovulation induction experiment, and divided into three numbered 5 mL flip-top centrifuge tubes. The tubes were then frozen and stored, and the chromium content in the fish was determined by ICP-MS.

[0150] 3. Results and Discussion

[0151] from Figure 5 It can be seen that, compared with the 8th hour of accumulation, the experimental group at the 24th hour of ovulation induction significantly reduced the chromium content in the fish.

[0152] Test Example 4 This invention relates to a device that utilizes hydrogen nanoparticle water to promote the excretion of heavy metals from biological systems, and its safety testing is being conducted on the excretion of heavy metals from biological systems.

[0153] Prepare fish tank water: Use a 100 L plastic bucket, with a volume ratio of tap water to deionized water of approximately 3:1. Aerate for 24 hours until the ORP is around 230 mV.

[0154] 1. Laboratory animals

[0155] Five zebrafish, measuring 1.8–2.5 cm in length, were selected and cultured in 30 L of aquarium water at 25 °C. They were fed brine shrimp larvae once daily, and their condition was checked regularly, with excrement and dead fish removed promptly. Water level changes were monitored, and water was replenished as needed to account for evaporation. Feeding was stopped 24 hours prior to the experiment.

[0156] Add 30L of fish-raising water to the rearing tank of the device in Example 1, and put in 5 fish for a 24-hour ovulation induction experiment. The ovulation induction experiment conditions are as follows:

[0157] (1) Control the dissolved oxygen content in the water of the feeding tank to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms;

[0158] (2) When the dissolved oxygen content in the water in the feeding tank drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms.

[0159] (3) When the hydrogen content in the water of the feeding tank drops below 0.1 mg / L, restart the device to promote the excretion of heavy metals from the organisms.

[0160] (4) Repeat the shutdown and startup steps (2)-(3) for 24 hours.

[0161] Observations of the zebrafish's health status showed no significant difference before and after the ovulation induction experiment.

[0162] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. The application of hydrogen nano-bubble water in promoting the excretion of heavy metals from organisms, wherein the application involves placing an organism to which the heavy metals to be excreted into a rearing tank, introducing hydrogen nano-bubble water into the rearing tank, and using the hydrogen nano-bubble water to promote the excretion of heavy metals from the organism, wherein the organism to which the heavy metals to be excreted is a fish. The preparation conditions for the water in the rearing tank are as follows: tap water and deionized water are mixed at a volume ratio of 2-3:1, aerated for 24-32 h, and the oxidation-reduction potential is controlled at 210-230 mV. The average particle size of the hydrogen nanobubble water is 400-600 nm, and the number density of hydrogen nanobubbles in the rearing tank water is 1×10⁻⁶. 7 ~1×10 9 The number of individuals / mL, the hydrogen content in the rearing tank water is 0.5~0.9 mg / L, the dissolved oxygen content is 4~8 mg / L, and the pH of the water is 6~9; The heavy metals include any one or a combination of copper, cadmium, hexavalent chromium, zinc, aluminum, manganese, and silver.

2. The application as described in claim 1, wherein the rearing tank is equipped with an aeration device.

3. The application as described in any one of claims 1-2, wherein the application can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 40%.

4. The application as described in claim 3, wherein the application can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 50%.

5. The application as described in claim 4, wherein the application can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 60%.

6. The application as described in claim 5, wherein the application can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 70%.

7. A device for promoting the excretion of heavy metals from organisms using hydrogen nanobubble water, and a method for promoting the excretion of heavy metals from organisms, wherein the organism to be excreted of heavy metals is a fish; The water preparation conditions for the breeding tank are as follows: mix tap water and deionized water at a volume ratio of 2-3:1, aerate for 24-32 h, and control the oxidation-reduction potential at 210-230 mV. During the operation of the device that promotes the excretion of heavy metals from organisms, the hydrogen content in the feeding tank is 0.5~0.9 mg / L, the dissolved oxygen content is 4~8 mg / L, and the hydrogen nanobubble number density is 1×10⁻⁶. 7 ~1×10 9 The concentration of cells / mL and the pH of the water body is 6-9. The average particle size of the hydrogen nanobubble water is 400~600 nm; the device for promoting the excretion of heavy metals from the body using hydrogen nanobubble water includes: Proton exchange membrane electrolyzer, high-pressure hydrogen mixing pump, rearing tank, booster pump and water tank, The proton exchange membrane electrolyzer includes a hydrogen outlet, an oxygen outlet, and a water inlet; the high-pressure hydrogen mixing pump includes a water inlet, a hydrogen inlet, and a water outlet; the rearing tank includes an aeration device, a water inlet, and a water outlet; the water tank includes a vent, an air inlet, and a water outlet; and the booster pump includes a water inlet and an outlet. The hydrogen outlet is connected to the hydrogen inlet, and the oxygen outlet is connected to the water tank inlet; the electrolytic cell inlet is connected to the booster pump outlet; the hydrogen mixing pump outlet is connected to the feeding tank inlet; the feeding tank outlet is connected to the hydrogen mixing pump inlet; the aeration device is connected to the water tank exhaust port; and the booster pump inlet is connected to the water tank outlet. The method for promoting the excretion of heavy metals from the body specifically includes the following steps: (1) Add the organisms to be excreted of heavy metals into the breeding tank, control the dissolved oxygen content in the breeding tank water to be above 5 mg / L, and start the device to promote the excretion of heavy metals from the organisms. (2) When the dissolved oxygen content in the water in the feeding tank drops to below 4 mg / L, shut down the device that promotes the excretion of heavy metals from the organisms. (3) When the hydrogen content in the water of the feeding tank drops below 0.1 mg / L, restart the device to promote the excretion of heavy metals from the organisms. (4) Repeat the shutdown and startup steps (2)-(3) for 24-48 hours.

8. The method as described in claim 7, wherein the specific steps of activating the device for promoting the excretion of heavy metals from the body are as follows: s-1: Turn on the switch of the proton exchange membrane electrolyzer, and pump the water stored in the water tank into the proton exchange membrane electrolyzer to generate hydrogen and oxygen; at the same time, turn on the aeration device in the breeding tank. s-2: After hydrogen enters the high-pressure hydrogen mixing pump through the hydrogen inlet, the water in the breeding tank enters the high-pressure hydrogen mixing pump through the breeding tank outlet and the hydrogen mixing pump inlet. The hydrogen mixes with the fish water and is pressurized through the booster pipe to obtain hydrogen nano bubble water. s-3: Hydrogen nano bubble water enters the rearing tank through the outlet of the hydrogen mixing pump and the inlet of the rearing tank; s-4: Oxygen enters the water tank through the oxygen outlet and then enters the breeding tank through the water tank exhaust outlet.

9. The method as described in claim 7, wherein the specific step of shutting down the device for promoting the excretion of heavy metals from the body is: turning off the switch of the proton exchange membrane electrolyzer.

10. In the method of claim 7, during the stage of shutting off the device that promotes the excretion of heavy metals from the organism, the aeration device in the rearing tank is always kept on.

11. The method as described in claim 7, wherein the power of the aeration device is 20-25W.

12. The method of claim 7, wherein the water tank contains ultrapure water.

13. The method as described in claim 7, wherein the working pressure of the booster pump is 0.6~0.7 MPa.

14. The method according to any one of claims 7-13, wherein the heavy metal comprises any one or a combination of copper, cadmium, hexavalent chromium, zinc, aluminum, manganese, and silver.

15. The method according to any one of claims 7-13, wherein the method can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 40%.

16. The method of claim 15, wherein the method can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 50%.

17. The method of claim 16, wherein the method can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 60%.

18. The method of claim 17, wherein the method can reduce the heavy metal content in the organism to which heavy metals are to be expelled by more than 70%.

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