An agricultural fungicidal composition containing WML-01 and kiralate, preparation and application thereof

Abstract

The application discloses a bactericidal composition containing WML-01 and kiral. Active ingredients of the bactericidal composition include WML-01 and kiral, and the mass ratio of the WML-01 to the kiral is 10:1-1:10. The bactericidal composition can be used for preventing and treating rice sheath blight, wheat foot rot, wheat scab and plant root rot, can effectively solve the problems of less bactericides for preventing and treating the diseases, unstable prevention and treatment effect, higher prevention and treatment cost and drug resistance in current production, and has a wide market application prospect.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and in particular to an agricultural fungicide composition containing WML-01 and cyazofamid, its formulation and application. Background Technology

[0002] Rice bakanae disease, also known as excessive growth disease, is a significant disease affecting rice production and occurs in all major rice-producing areas of my country. The main pathogen is *Fusarium oxysporum* (Fusarium oxysporum). Fusarium fujikuroi Infected seeds and diseased plant debris in the field are the main primary sources of infection for rice bakanae disease. Infected grains often fail to germinate or emerge after sowing. Typically, infected seedlings are thinner and taller than healthy seedlings, with slender leaf sheaths, pale yellow leaves, and poor root development; some seedlings die before transplanting. After transplanting, the mycelium can spread throughout the entire plant under suitable conditions, stimulating excessive vegetative growth. During flowering, the pathogen spreads to the flowers, invading the glumes and endosperm, causing shriveled or deformed grains. If the pathogen invades late, although the grains may not show symptoms, the mycelium has already penetrated internally, making the seeds infected and affecting subsequent rice planting, causing significant damage. Chemical control remains the primary method for controlling rice bakanae disease. Currently, fungicides registered for the control of rice bakanae disease on the market include carbendazim, prochloraz, cyazofamid, and fludioxonil. However, the long-term, large-scale, and frequent use of chemical agents has led to pathogens gradually developing resistance to different fungicides, resulting in a significant reduction in the effectiveness of the agents. Increasing the amount of pesticides used will lead to problems such as pesticide residues, seed safety, and environmental pollution.

[0003] Wheat stem base rot, commonly known as "crown rot" or "dryland foot disease," is caused by Fusarium graminearum (…). Fusarium pseudograminearum Fusarium graminearum ( ), Fusarium graminearum This global wheat stem rot is caused by various Fusarium fungi, including *Fusarium graminearum*, and is the dominant pathogen in major wheat-producing areas of my country. Early symptoms of stem rot cause browning and rotting of young roots or coleoptiles, leading to seedling death in severe cases. The disease then spreads to the first and second stem nodes, causing the affected areas to turn dark brown or rot, the leaf sheaths at the stem base to wither, and the stem to turn brown and necrotize. Later, the browning at the stem base hinders the transport of water from the roots to the ear, resulting in a completely white ear. After the ear forms a white head, the wheat grains cannot continue to develop, significantly impacting the grain filling rate and yield. Currently, only eight pesticides are registered for the control of this disease, including difenoconazole, tebuconazole, azoxystrobin, prothioconazole, trifluralin, and thiamethoxam. There is an urgent need for new pesticides to control this disease.

[0004] Fusarium head blight, commonly known as "wheat rot," is a global wheat disease caused by various Fusarium fungi, including Fusarium graminearum.F. graminearum ) is the dominant pathogen in many wheat-producing areas. Wheat scab occurs in all regions of China, causing seedling blight, stem rot and ear rot, and the disease incidence often reaches more than 50% in epidemic years. Not only does it greatly reduce yield and quality, but the diseased kernels also contain toxins that can cause poisoning in humans and animals. Due to the lack of resistant germplasm resources, chemical control is still the key to controlling wheat scab. The fungicides used to control wheat scab in production mainly include benzimidazole fungicides, triazole fungicides, methoxy acrylate fungicides, and methoxy acrylate fungicides. The long-term and large-scale use of chemical fungicides has led to increasing resistance of wheat scab pathogens.

[0005] Root rot is a common disease in plant production, and the pathogen mainly damages the roots of plants, causing poor plant growth, seed rot, root rot, dwarfing, yellowing, and even whole plant death, resulting in serious yield loss. Among them, soybean root rot, the main root disease in soybean production, is mainly infected by Fusarium, Pythium and Rhizoctonia solani, etc. The disease occurs in seedling stage, affecting the growth of seedlings and even causing seedling death, reducing the number of seedlings in the field. In the plant stage, the growth and number of root nodules are affected due to root damage, causing poor growth of the aboveground part, dwarfing, affecting the number of pods and grain weight, and thus leading to yield reduction. Currently, the fungicides registered for the control of soybean root rot on the market include difenoconazole, prochloraz, fludioxonil, carbendazim, thiram, etc.

[0006] Phenamacril is a 2-cyano acrylate fungicide developed by Jiangsu Pesticide Research Institute, which is effective against Fusarium diseases, especially wheat scab. The fungicide acts on the myosin-5 of Fusarium graminearum, strongly inhibiting the growth and development of fungal hyphae, and has both protective and curative effects. Studies have shown that phenamacril can not only control wheat scab but also reduce the toxin content in wheat ear grains. Currently, there are 10 pesticide products containing phenamacril registered in China, of which 4 can be used to control scab. Due to the single action site of phenamacril, long-term and continuous use can easily lead to the occurrence of drug resistance. At present, the rice sheath blight fungus in some rice planting areas of Northeast China, Anhui and Zhejiang has developed obvious resistance to phenamacril.

[0007] At present, there are few fungicides for the control of rice sheath blight, wheat foot rot, wheat scab and plant root rot caused by Fusarium. With the long-term and large-scale use of fungicides with single mechanism of action, the pathogen has developed resistance to varying degrees. The development of new fungicides with novel mechanisms of action and their rational combination with commonly used fungicides through scientific compounding modes can not only improve efficacy but also delay the occurrence of drug resistance, which is of great significance for controlling diseases and prolonging the life cycle of fungicides. SUMMARY

[0008] The present application aims to provide an agricultural fungicidal composition containing WML-01 and acypetacs, a preparation and application thereof, so as to solve the technical problems of the current prevention and treatment of rice false smut caused by fusarium, wheat basal stem rot, wheat scab and plant root rot, and the prevention and treatment agents are less in variety, and with the long-term use of the single selective agents with single mechanism, the pathogenic bacteria have developed drug resistance to different degrees.

[0009] In order to achieve the object of the present application, the first aspect of the present application provides a fungicidal composition, the active ingredient of which comprises WML-01 and acypetacs, and the mass ratio of the WML-01 to the acypetacs is 10:1-1:10, wherein the WML-01 is a butenolide compound containing thiazolidine structure, and the structural formula thereof is shown in formula I:

[0010] .

[0011] In an alternative embodiment, the mass ratio of the WML-01 to the acypetacs is 10:1-1:3.

[0012] In an alternative embodiment, the mass ratio of the WML-01 to the acypetacs is 5:1-2:1.

[0013] The second aspect of the present application provides a fungicidal preparation, the active ingredient of which is the fungicidal composition.

[0014] In an alternative embodiment, the dosage form of the fungicidal preparation is seed coating agent, suspension agent, water emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano preparation, granule, wettable powder or water dispersible granule.

[0015] In an alternative embodiment, the components of the fungicidal preparation further comprise an auxiliary agent or a carrier for assisting the fungicidal composition, and the auxiliary agent is xanthan gum, wetting agent, dispersing agent, defoaming agent, antifreeze or warning color.

[0016] In an alternative embodiment, the wetting agent is nonylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether or alkyl naphthalene sulfonate; the dispersing agent is sodium salt of alkyl naphthalene sulfonate polycondensate or sulfonate of alkyl naphthalene sulfonate polycondensate; the defoaming agent is n-octanol or organosilicon; the antifreeze is ethylene glycol; and the warning color is basic roseine, gold red or fast peach red.

[0017] The third aspect of the present application provides an application of the fungicidal composition or the fungicidal preparation in the prevention and treatment of plant diseases, and the plant diseases are rice false smut ( Fusarium fujikuroi ), wheat basal stem rot ( Fusarium pseudograminearum ), wheat scabFusarium graminearum ) and plant root rot ( Fusarium spp . ).

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The agricultural fungicide composition provided by this invention, which uses WML-01 and cyazofamid as active ingredients, has a synergistic effect on the control of rice seedling blight, wheat stem base rot, wheat scab, and plant root rot. The use of this fungicide composition can effectively improve the disease control effect, reduce the amount of pesticide used, delay the occurrence of pesticide resistance, and increase crop yield, and has broad market application prospects. Detailed Implementation

[0020] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0021] Agricultural fungicidal compositions and their formulations can be applied in various ways, and this invention does not impose any particular limitation. One or a combination of various application methods can be used, such as seed coating, seed soaking, spraying, root drenching, broadcasting, hole application, and furrow application. Depending on crop cultivation, formulation type, and target pests, seed coating, seed dressing, and seed soaking are preferred methods for this fungicidal agricultural composition.

[0022] Example 1: In vitro antibacterial activity of WML-01 and cyazofamid combined against rice bakanae disease pathogens, wheat stem rot pathogens, wheat scab pathogens, and soybean root rot pathogens.

[0023] 1. Test materials

[0024] 1.1 Test strains

[0025] Rice seedling blight pathogen ( Fusarium fujikuroi (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0026] Soybean root rot pathogen ( Fusarium oxysporum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0027] Wheat stem rot fungus ( Fusarium pseudograminearum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0028] Fusarium head blight of wheat ( Fusarium graminearum (Provided by the College of Plant Protection, China Agricultural University), identified using conventional methods.

[0029] 1.2 Test reagents

[0030] WML-01 (99% technical material), provided by School of Science, China Agricultural University. The compound and its preparation method are disclosed in patent publication No. CN 118724887 A, and the compound is numbered as 3-1.

[0031] The structural formula is shown in formula I:

[0032]

[0033] Cyazofamid (95% technical material), product of Jiangsu Pesticide Research Institute Co., Ltd.

[0034] The above pesticide technical material is prepared into a mother liquor with a concentration of 10 4 mg / L by using dimethyl sulfoxide as a solvent, and the mother liquor is stored in a 4°C refrigerator for standby use.

[0035] 1.3 Test medium

[0036] Potato dextrose agar (PDA): 200 g of potato, 18 g of glucose, 12 g of agar powder, and 1000 mL of distilled water.

[0037] 2 Test method

[0038] The test is carried out in accordance with NY / T 1156.2-2006 Guidelines for Pesticide Indoor Biological Test Inhibition of pathogenic fungi mycelium growth test plate method. The main operation process of the plate method is as follows: 5 concentration gradients are set between 10% and 90% of the inhibition rate of the pesticide on the target fungus, then a puncher with a diameter of 6 mm is used to punch a fungus cake on the same circumference near the edge of the activated target fungus (to ensure that the fungus age of the same repeat test pathogenic bacteria is the same), and a inoculation needle is used to inoculate the fungus cake to the center of the series of drug-containing medium plates under sterile conditions, with the mycelium facing down, and a plate without drug is set as a control, and placed in the dark for culture. Each treatment is repeated 3 times for determination. The colony diameters of each concentration treatment are recorded, and the mycelium growth inhibition rate is calculated. Then the inhibition rate is converted into a probability value (Y), and the concentration of the pesticide is converted into a logarithmic value with 10 as the base (X), and X-Y is made into a regression straight line, and the toxicity regression curve equation Y=a+bX of each pesticide on the target fungus, as well as the correlation coefficient r and the effective inhibition concentration EC 50 .

[0039] The joint toxicity of the compound preparation is represented by the method of co-toxicity coefficient of Sun Yunpei:

[0040] Theoretical toxicity index of compound preparation TTI =∑(toxicity index of a certain drug x percentage of active ingredient of the drug in the mixture).

[0041]

[0042] CTC ≧120 synergistic effect; CTC ≦ 80 antagonistic effect; 80 CTC <120 additive effect.

[0043] 3 Test results

[0044] Table 1 In vitro antibacterial activity of WML-01 and kiralos combined against Pythium aphanidermatum

[0045]

[0046] The above results show that: WML-01 and kiralos in the range of 10:1~2:1 (w / w) of the complex, the co-toxicity coefficient of Pythium aphanidermatum is between 106.61~162.61, showing additive and synergistic effect. Among them, when the mass ratio of WML-01 and kiralos is in the range of 5:1~2:1, the co-toxicity coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 162.61.

[0047] Table 2 In vitro antibacterial activity of WML-01 and kiralos combined against Rhizoctonia cerealis

[0048]

[0049] The above results show that: WML-01 and kiralos in the range of 10:1~1:10 (w / w) of the complex, the co-toxicity coefficient of Pythium aphanidermatum is between 92.78~173.13, showing additive and synergistic effect. Among them, when the mass ratio of WML-01 and kiralos is in the range of 10:1~1:3, the co-toxicity coefficient is greater than 120, showing synergistic effect, and the synergistic effect is significant, and the highest can reach 173.13.

[0050] Table 3 In vitro antibacterial activity of WML-01 and kiralos combined against Gibberella zeae

[0051]

[0052] The above results show that: WML-01 and kiralos in the range of 10:1~1:10 (w / w) of the complex, the co-toxicity coefficient of Pythium aphanidermatum is between 106.06~140.37, showing additive and synergistic effect. Among them, when the mass ratio of WML-01 and kiralos is in the range of 10:1~1:2, the co-toxicity coefficient is greater than 120, showing synergistic effect.

[0053] Table 4 In vitro antibacterial activity of WML-01 and kiralos after compounding against soybean root rot fungus

[0054]

[0055] The above results show that: when WML-01 and kiralos are compounded in a mass ratio of 10:1~1:10 (w / w), the co-toxicity coefficients of the plant root rot fungus are between 97.22~172.68, showing additive and synergistic effects. When the mass ratio of WML-01 and kiralos is in the range of 10:1~1:3, the co-toxicity coefficients are all greater than 120, showing synergistic effects, and the synergistic effects are significant, with the highest reaching 172.68.

[0056] Example 2 Field control effect of WML-01 and kiralos compounding on wheat scab

[0057] 1. Materials and methods

[0058] 1.1 Test materials

[0059] 15% WML-01 ·kiralos suspension concentrate, self-made, wherein WML-01 10%, kiralos 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand milling process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand milling process; 25% kiralos suspension concentrate, product of Jiangsu Pesticide Research Institute Co., Ltd.

[0060] 1.2 Test site profile

[0061] The test was conducted in Fengtai County, Anhui Province, with clay loam soil, medium fertility, wheat variety Huaiyumi No. 1, and seed rate of 25 kg per mu.

[0062] 1.3 Test design

[0063] Table 5 Test scheme of seed treatment on wheat scab

[0064]

[0065] 1.4 Investigation method and calculation

[0066] Each treatment area was randomly sampled at 5 points, with 100 ears per point, and the disease index and control effect were calculated by grading the percentage of ear area affected by scab.

[0067] Scab grading standards:

[0068] 0 grade, no disease on whole ear;

[0069] 1 grade, the area of withered ear is less than 1 / 4 of the whole ear;

[0070] 3 grade, the area of withered ear is 1 / 4-1 / 2 of the whole ear;

[0071] 5 grade, the area of withered ear is 1 / 2-3 / 4 of the whole ear;

[0072] 7 grade, the area of withered ear is more than 3 / 4 of the whole ear;

[0073] Pharmacodynamic calculation method:

[0074] Disease index (%) =∑(number of plants (ears) of each grade × representative value of each grade) / (total number of plants (ears) surveyed × representative value of the highest grade) × 100

[0075] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area × 100

[0076] 2 Test results

[0077] Table 6. Control effect of the fungicide treatment on wheat scab

[0078]

[0079] Note: * The values are the average of 4 repetitions; ** The significance level of variance analysis is p=0.05.

[0080] From the contents in the above table, after 2 times of spraying, the high dose 200 ml treatment (effective ingredient 30 g) of 15% WML-01·ambush suspension agent has a control effect of 91.90% on wheat scab, which is better than the single dose treatment of ambus (effective ingredient 50 g) and the single dose treatment of WML-01 (effective ingredient 20 g); the low dose 100 ml treatment (effective ingredient 15 g) has a control effect of 84.85% on wheat scab, which is higher than the single dose treatment of WML-01 and ambus.

[0081] The above results show that the combination of WML-01 and ambus has excellent control effect on wheat scab, and the field dosage is significantly reduced.

[0082] Example 3. Field control effect of WML-01 and ambus combination on soybean root rot

[0083] 1. Materials and methods

[0084] 1.1 Test material

[0085] 15% WML-01·acypetraf 5% SC, self-made, wherein WML-01 10%, acypetraf 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand mill process; 10% WML-01 SC, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand mill process; 25% acypetraf SC, product of Jiangsu Pesticide Institute Co., Ltd.

[0086] 1.2 Test site profile

[0087] The test site was located in the modern agricultural demonstration park of Beian City. The soil type was black soil, the organic matter content was 5.6%, and the pH value was 6.6. Sowing was carried out on May 21, and the 65 cm ridge three cultivation mode was adopted, with 320,000 seedlings per mu, 48% special fertilizer for soybeans, and the fertilizer amount was 320 kg per mu (N:P:K=15:23:10). The seedlings emerged on June 1. The soil moisture was good. The previous crop was soybean. It relied on natural precipitation.

[0088] 1.3 Test design

[0089] Table 7 Test scheme of seed treatment on soybean root rot

[0090]

[0091] 1.4 Investigation method and calculation

[0092] Soybean root rot investigation

[0093] Each investigation was carried out once after 30 d and 60 d of soybean emergence, respectively. Not less than 30 soybean plants were randomly dug in each plot for investigation, and the total number of plants and the number of plants at each level were recorded. The incidence of root rot was investigated, and the control effect was calculated.

[0094] Disease classification: (root lesion area)

[0095] 0 level: main root, fibrous root is healthy, no lesion, nodule is more;

[0096] 1 level: there are sporadic lesions on the main root, but not in pieces, and there are a few lesions on the fibrous root;

[0097] 3 level: main root lesion is in pieces, but less than 1 / 4 of the root area, and fibrous root is slightly diseased;

[0098] 5 level: main root lesion is between 1 / 4 and 1 / 2 of the root area, and fibrous root is more diseased, but not in pieces;

[0099] 7: The area of the main root disease spot is between 1 / 2 and 1 / 4 of the root area, the root hair disease spot is in pieces, and part of the root hair falls off;

[0100] 9: The whole root is surrounded by disease spots, and the root is rotten, and the root hair is almost none.

[0101] Pharmacodynamic calculation method:

[0102] Disease index (%) =∑(number of each level of diseased plants (ears) x each level of representative value) / (total number of plants (ears) surveyed x highest level of representative value) x 100

[0103] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area x 100

[0104] 2 Test results

[0105] Table 8 Effect of fungicide treatment on soybean root rot

[0106]

[0107] Note: * The average of 4 repetitions; ** The significance level of variance analysis is p = 0.05.

[0108] From the above table, it can be seen that 30 days after emergence, the 15% WML-01 · acypetrex suspension agent high dose 200 ml seed treatment (effective ingredient 30 g) has a prevention effect of 80.61% on soybean root rot, which is significantly better than WML-01 single agent (effective ingredient 20 g) and acypetrex single agent treatment (effective ingredient 50 g); the 15% WML-01 · acypetrex suspension agent low dose 100 ml treatment (effective ingredient 15 g) has a prevention effect of 74.20% on soybean root rot, which is significantly better than acypetrex single agent seed treatment and equivalent to WML-01 single agent treatment.

[0109] 60 days after emergence, the 15% WML-01 · acypetrex suspension agent 100 ml and 200 ml seed treatment has excellent prevention effect on soybean root rot, which is 79.51% and 85.62% respectively, which is significantly better than WML-01 single agent and acypetrex single agent treatment.

[0110] The above results show that the combination of WML-01 and acypetrex shows excellent prevention effect on soybean root rot, and the field drug dosage is significantly reduced.

[0111] Example 4 Field prevention effect of WML-01 and acypetrex combination on wheat foot rot

[0112] 1 Materials and methods

[0113] 1.1 Test materials

[0114] 15% WML-01 + acypetacs SC, self-made, wherein WML-01 10%, acypetacs 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 10% WML-01 SC, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is mass percentage content, prepared by sand mill process; 25% acypetacs SC, product of Jiangsu Pesticide Institute Co., Ltd.

[0115] 1.2 Test site profile

[0116] The test was set in Feixiang District, Handan City, Hebei Province, with clay loam soil, medium fertility, and wheat variety Woyu No. 3, with a seed rate of 20 kg per mu.

[0117] 1.3 Test design

[0118] Table 9 Test scheme of seed treatment on wheat stem base rot

[0119]

[0120] 1.4 Investigation method and calculation

[0121] Stem base rot investigation

[0122] a) Investigation of control effect at the filling stage of stem base rot

[0123] Randomly sample 5 points per treatment area, 100 plants per point, investigate the number of diseased plants and disease index, and calculate disease index and control effect.

[0124] Stem base rot grading standards:

[0125] 0 level, plant not diseased;

[0126] 1 level, stem brown in the ground;

[0127] 3 level, first internode brown;

[0128] 5 level, second internode brown;

[0129] 7 level, third internode appears brown symptoms, but no white head;

[0130] 9 level, white head or plant dead due to disease.

[0131] Pharmacodynamic calculation method:

[0132] Disease index (%) =∑(number of diseased plants (ears) at each level x representative value at each level) / (total number of plants (ears) surveyed x representative value at the highest level) x 100

[0133] Control effect (%) = (disease index after treatment in the control area - disease index after treatment in the treatment area) / disease index after treatment in the control area x 100

[0134] b) White ear rate survey during the grain-filling stage

[0135] Each treatment area was randomly sampled at 5 points, with 100 plants at each point, to survey the total ear number and white ear number, and the control effect was calculated.

[0136] White ear rate (%) = white ear number / total number of plants surveyed x 100

[0137] Control effect (%) = [white ear rate in the control area - white ear rate in the treatment area with the fungicide] / white ear rate in the control area x 100

[0138] 2 Test results

[0139] Table 10. Control effect of the fungicide treatment on wheat foot rot

[0140]

[0141] Note: * The values are the average of 4 replicates; ** The significance level of variance analysis is p = 0.05.

[0142] From the above table, it can be seen that during the grain-filling stage, the high-dose 200 ml seed treatment with 15% WML-01·kiralos (30 g of active ingredient) had a control effect of 87.31% on wheat foot rot, which was significantly better than the single-dose treatment with WML-01 (20 g of active ingredient) and kiralos (50 g of active ingredient); the low-dose 100 ml treatment (15 g of active ingredient) had a control effect of 83.70% on wheat foot rot, which was significantly better than the single-dose seed treatment with kiralos and comparable to the single-dose treatment with WML-01.

[0143] The white ear rate survey results showed that the high-dose 200 ml treatment with 15% WML-01·kiralos had a control effect of 88.92% on white ears, and the low-dose 100 ml treatment had a control effect of 84.75% on white ears, which was significantly better than the single-dose treatment with kiralos.

[0144] The above results show that after the combination of WML-01 and kiralos, the control effect on wheat foot rot is excellent at a significantly lower dose than the single-dose treatment with kiralos, and when the dose is reduced, the control effect is slightly higher than that of the single-dose treatment with WML-01.

[0145] Example 5 Field control effect of WML-01 and kresoxim-methyl on rice seedling bacterial wilt

[0146] 1. Materials and methods

[0147] 1.1 Test materials

[0148] 15% WML-01 ·kresoxim-methyl suspension concentrate, self-made, wherein WML-01 10%, kresoxim-methyl 5%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand mill process; 10% WML-01 suspension concentrate, self-made, wherein WML-01 10%, Morwet D-425 3%, Witconol NP-100 1%, xanthan gum 0.3%, water balance, % is the mass percentage content, prepared by sand mill process; 25% kresoxim-methyl suspension concentrate, product of Jiangsu Pesticide Research Institute Co., Ltd.

[0149] 1.2 Test site profile

[0150] The test was set in Zhao Yuan Village, Yaojiang Town, Zhuji City, Zhejiang Province, with clay loam soil, medium fertility, and medium-early 39 as the rice variety, 5 kg per mu of seed quantity, and seedling transplanting.

[0151] 1.3 Test design

[0152] Table 11 Test scheme of seed treatment on rice seedling bacterial wilt

[0153]

[0154] 1.4 Investigation method and calculation

[0155] Seedling bacterial wilt: randomly sample 5 points per plot in the seedbed, investigate 10 cm*10 cm per point, repeat 3 times, record the disease rate, and calculate the control effect.

[0156] Disease rate (%) = number of diseased plants / total number of plants investigated × 100%

[0157] Control effect (%) = (disease rate of blank control area - disease rate of treatment area) / disease rate of blank control area × 100%

[0158] 2. Test results

[0159] Table 12 Control effect of seedbed treatment with pesticides on rice seedling bacterial wilt

[0160]

[0161] Note: * The average value of 4 repetitions; ** The significance level of variance analysis is p=0.05.

[0162] From the above table, it can be seen that during the seedling stage, the high-dose 1000-fold WML-01·acrinachloridin suspension agent seed soaking treatment has a 97.30% control effect on rice seedling disease; the low-dose 2000-fold liquid seed soaking treatment has a 95.61% control effect on rice seedling disease; the WML-01 single agent 1000-fold liquid seed soaking treatment has a 90.98% control effect on rice seedling disease, all of which are significantly better than the acrinachloridin 2000-fold seed soaking treatment.

[0163] Because the current field rice seedling disease fungus has a serious drug resistance, the 25% acrinachloridin suspension agent 2000-fold liquid recommended by the manufacturer basically has no control effect. The WML-01 and acrinachloridin compound show excellent control effect on seedling disease, which is a good way to solve the development of seedling disease drug resistance, and the field drug dosage is significantly reduced.

[0164] Although the present application has been described in detail by general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application are within the scope of the present application.

Claims

1. A fungicidal composition, characterized by, The active ingredient of the fungicidal composition comprises WML-01 and kiralate, wherein the mass ratio of the WML-01 and kiralate is 10:1-1:3; the structural formula of the WML-01 is shown in formula I: 。 2. The bactericidal composition according to claim 1, characterized by, The mass ratio of the WML-01 and kiralate is 5:1-2:

1.

3. A bactericidal preparation, characterized by, The fungicidal preparation comprises the fungicidal composition of claim 1.

4. The bactericidal preparation according to claim 3, characterized in that, The dosage form of the fungicidal preparation is seed coating agent, suspension agent, water emulsion, microemulsion, emulsifiable concentrate, microcapsule suspension preparation, nano preparation, granule, wettable powder or water dispersible granule.

5. The sterilizing preparation according to claim 3, characterized in that, The components of the fungicidal preparation further comprise auxiliary agents for assisting the fungicidal composition, and the auxiliary agents are xanthan gum, wetting agent, dispersing agent, defoaming agent, antifreezing agent or warning color.

6. The bactericidal preparation according to claim 5, characterized in that, The wetting agent is alkyl phenol polyoxyethylene ether or alkyl naphthalene sulfonate; the dispersing agent is sodium salt of alkyl naphthalene sulfonate polycondensate or sulfonate of alkyl naphthalene sulfonate polycondensate; the defoaming agent is n-octanol or organic silicon; the antifreezing agent is ethylene glycol; and the warning color is basic roseine, gold light red or fast peach red.

7. The use of the fungicidal composition according to any one of claims 1-2 or the fungicidal preparation according to any one of claims 3-6 in the control of plant diseases caused by Fusarium fujikuroi, wherein the mass ratio of WML-01 to kiralyl in the fungicidal composition or the fungicidal preparation is 5:1 to 2:

1. Fusarium fujikuroi ​​ or the plant disease is caused by Fusarium pseudograminearum Fusarium pseudograminearum wheat seedling blight, the mass ratio of WML-01 and kiral in the fungicidal composition or the fungicidal preparation is 10:1-1:3; or the plant disease is wheat scab caused by Fusarium graminearum Fusarium graminearum WML-01 and kiral, the mass ratio of which in the fungicidal composition or the fungicidal preparation is 10:1-1:2; or the plant disease is soybean root rot caused by Fusarium oxysporum Fusarium oxysporum WML-01 and kiral, and the mass ratio of WML-01 to kiral in the fungicidal composition or the fungicidal preparation is 10:1-1:3.

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