A qi and blood nourishing compound composition, a preparation method and application thereof

Abstract

The present application belongs to the field of food technology, and particularly relates to a qi and blood nourishing compound composition, a preparation method and application thereof. The raw materials of the qi and blood nourishing compound composition include 10-20 parts of red ginseng powder, 5-20 parts of mulberry powder, 5-20 parts of Chinese wolfberry powder, 5-15 parts of tremella polysaccharide, 5-15 parts of blood protein polypeptide and 0.5-2 parts of ferrous gluconate by weight. The composition of the present application promotes the generation of qi and blood, regulates the running of qi and blood, and at the same time enhances the antioxidant and anti-fatigue ability of the body, thereby providing a more effective solution for improving the qi and blood deficiency type sub-health state.

Description

Technical Field

[0001] This invention belongs to the field of food technology, specifically relating to a qi-tonifying and blood-nourishing compound composition, its preparation method, and its application. Background Technology

[0002] Qi and blood, as crucial concepts in Traditional Chinese Medicine, are the material basis for maintaining the body's normal functions. Qi has functions such as promoting, warming, defending, consolidating, and transforming, while blood nourishes the internal organs and limbs and is the material basis for mental activities. At the same time, "Qi is the commander of blood, and blood is the mother of Qi," and "blood is generated from qi, and qi is transformed from blood." Qi and blood are mutually transformative, interconnected, and interdependent.

[0003] In Traditional Chinese Medicine (TCM) theory, "deficiency of both Qi and Blood" is a syndrome name, also known as Qi and Blood insufficiency. Its essence is due to excessive consumption and insufficient production of Qi, coupled with blood loss or Qi deficiency leading to impaired blood production, resulting in a deficiency of both Qi and Blood. Clinically, it manifests as the simultaneous presence of Qi and Blood deficiency, with symptoms such as shortness of breath, fatigue, lethargy, dizziness, palpitations, insomnia, pale or sallow complexion, and a pale tongue with a white coating.

[0004] Qi and blood deficiency is mainly caused by the following aspects: Regarding qi, it manifests primarily as impaired qi generation, including insufficient qi production due to impaired lung function, impaired spleen and stomach function leading to transportation disorders, and deficiency of congenital qi due to declining kidney qi absorption function; secondly, excessive qi depletion, mainly caused by prolonged fatigue and chronic diseases. Regarding blood, it manifests primarily as a dual imbalance between production and consumption. Firstly, there is insufficient blood supply, encompassing spleen and stomach weakness preventing the provision of sufficient hematopoietic raw materials, kidney essence deficiency leading to decreased bone marrow hematopoietic function, insufficient lung qi affecting blood distribution leading to insufficient blood production, and a lack of essential iron and various proteins, carbohydrates, and vitamins necessary for hematopoiesis; secondly, there is abnormal blood loss, with blood loss and blood stasis leading to excessive blood loss, and blood stasis hindering the generation of new blood and further depleting qi and blood.

[0005] In recent years, with the accelerated pace of life and increased pressure, more and more people are experiencing a sub-healthy state characterized by qi and blood deficiency due to various unhealthy lifestyle habits such as excessive fatigue, irregular diet, and disordered work and rest. If qi and blood are not replenished in time, it will lead to decreased immunity, physical weakness, and other problems, affecting people's normal lives.

[0006] In Western medicine, the main ways to improve qi and blood deficiency include nutritional supplementation and drug therapy. Directly supplementing deficiencies with iron and vitamins can improve anemia and malnutrition, but this is often accompanied by absorption problems, slow effectiveness, and gastrointestinal irritation. Chemical drugs have good clinical efficacy; for example, glucocorticoids can significantly increase hemoglobin levels and improve the blood microenvironment in the treatment of autoimmune hemolytic anemia, but long-term use can cause metabolic disorders, weakened immunity, and inhibition of bone growth and development. These Western medical treatments have many adverse reactions and toxic side effects, and their safety cannot be ignored.

[0007] In Traditional Chinese Medicine (TCM), its long history and rich experience have given its Qi and Blood regulation system a unique advantage. The core lies in the organic unity of holistic concepts and syndrome differentiation and treatment. Through Yin-Yang balance and early intervention in "prevention of disease," it regulates Qi transformation, unblocks meridians and blood flow, and restores organ coordination, thereby improving Qi and Blood. Compared to the targeted intervention strategies of Western medicine, TCM is gentler and more natural, fundamentally improving the patient's constitution and effectively regulating the body's self-repair capabilities. Chinese invention patent application CN120285099A discloses a TCM composition containing ingredients such as Codonopsis pilosula, Salvia miltiorrhiza, Schisandra chinensis, and Ophiopogon japonicus. Chinese invention patent application CN118383513A discloses a compound composition containing ingredients such as Polygonatum sibiricum, Poria cocos, Dioscorea opposita, and Codonopsis pilosula. However, both technologies suffer from unavoidable problems inherent in the traditional methods of using Chinese medicinal materials: cumbersome decoction, time-consuming and labor-intensive processes, difficulty in guaranteeing the dissolution rate of effective ingredients, unstable efficacy, and unpleasant taste, with the bitterness of the herbs affecting consumer acceptance.

[0008] Most current functional foods for replenishing qi and blood focus on a single function, emphasizing a single benefit. However, according to the interdependent relationship between qi and blood in Traditional Chinese Medicine, combating fatigue is closely related to replenishing qi and blood; prolonged fatigue depletes qi and damages blood, creating a vicious cycle. Therefore, there is a need to research a compound formula that combines qi-tonifying and blood-nourishing ingredients to combat fatigue. Summary of the Invention

[0009] To address the shortcomings of existing technologies, this invention provides a Qi-tonifying and blood-nourishing compound composition, its preparation method, and its applications. This composition promotes the generation and regulation of Qi and blood, while simultaneously enhancing the body's antioxidant and anti-fatigue capabilities, providing a more effective solution for improving sub-health conditions caused by Qi and blood deficiency.

[0010] This invention is achieved through the following technical solution:

[0011] A compound composition for invigorating qi and nourishing blood, wherein the raw materials of the compound composition for invigorating qi and nourishing blood include: 10-20 parts red ginseng powder, 5-20 parts mulberry powder, 5-20 parts wolfberry powder, 5-15 parts tremella polysaccharide, 5-15 parts blood protein polypeptide and 0.5-2 parts ferrous gluconate.

[0012] Preferably, the raw materials of the Qi-nourishing and blood-tonifying compound composition, by weight, include: 10-15 parts red ginseng powder, 10-15 parts mulberry powder, 15-20 parts wolfberry powder, 10-15 parts tremella polysaccharide, 10-15 parts blood protein polypeptide and 1-2 parts ferrous gluconate.

[0013] More preferably, the raw materials of the Qi-nourishing and blood-tonifying compound composition, by weight, include: 12 parts red ginseng powder, 17 parts wolfberry powder, 12 parts mulberry powder, 13 parts tremella polysaccharide, 13 parts blood protein polypeptide and 1.4 parts ferrous gluconate.

[0014] Red ginseng is the dried root of Panax ginseng CAMey., a plant of the Araliaceae family, which is steamed and then dried. It is harvested in autumn, washed, steamed, and then dried.

[0015] Mulberries are primarily used for "nourishing yin and blood". Because of their cold nature, they can be combined with red ginseng to regulate qi and blood, balance cold and heat, and replenish blood and warm the middle jiao. Mulberries replenish yin and blood, while red ginseng replenishes yang qi. The combination of the two can improve symptoms of qi and blood deficiency.

[0016] Tremella polysaccharides can achieve the traditional Chinese medicine goal of "tonifying qi to generate blood" through multiple pathways, such as promoting hematopoiesis, protecting blood cells, improving iron utilization, and enhancing immunity.

[0017] Preferably, the molecular weight of the hemoprotein polypeptide is <1000 Da.

[0018] Preferably, the preparation method of the red ginseng powder includes the following steps: mixing red ginseng slices with water, extracting using a stepped temperature change method, filtering, concentrating, drying, and pulverizing to obtain red ginseng powder.

[0019] More preferably, the weight ratio of the red ginseng slices to water is 1:8-12; the stepped temperature variation is: extraction at 55-65℃ for 0.8-1.2h, extraction at 85-95℃ for 1.5-2.5h, and extraction at 55-65℃ for 0.8-1.2h; the concentration temperature is 50-60℃, and the concentration is carried out until the sugar content of the red ginseng extract is ≥60%.

[0020] Preferably, the preparation method of the tremella polysaccharide includes the following steps: pulverizing and sieving tremella, mixing it with phosphate buffer, stirring, adding a compound enzyme for enzymatic hydrolysis, centrifuging, concentrating the supernatant, precipitating with alcohol, washing, and drying to obtain the polysaccharide.

[0021] More preferably, the ratio of the tremella fuciformis to the phosphate buffer is 1g:20-40mL, the phosphate buffer is a sodium phosphate buffer with a concentration of 0.01-0.1M and a pH of 5.5-6.5, the stirring temperature is 50-55℃, the stirring time is 20-30min, the complex enzyme is chitinase and β-glucanase with a mass ratio of 1:1-2, the enzymatic hydrolysis temperature is 50-55℃, the pH is 5.5-6.0, and the enzymatic hydrolysis process is carried out in a constant temperature shaker at a speed of 150-200 rpm for 2-4h.

[0022] More preferably, the amount of the compound enzyme added is 2% of the mass of the dried tremella powder.

[0023] More preferably, after the enzymatic hydrolysis reaction is completed, the reaction solution is inactivated (90℃, 15min), cooled to room temperature, centrifuged at 4000-5000 rpm for 10-20min, and the supernatant is collected.

[0024] More preferably, the supernatant is concentrated to 1 / 4-1 / 5 of its original volume by rotary evaporation under reduced pressure at 55-65°C, yielding a concentrated solution. Anhydrous ethanol is slowly added to the concentrated solution while stirring, until the final volume fraction of ethanol reaches 70%-80%. The solution is then left to stand overnight at 4°C to allow the polysaccharides to fully precipitate.

[0025] More preferably, the precipitate is washed twice each with anhydrous ethanol and acetone to remove moisture, pigments, and small molecule impurities. The washed precipitate is then dried in a vacuum drying oven at 50-60℃ and pulverized to obtain Tremella polysaccharide.

[0026] The present invention also relates to a method for preparing the above-mentioned Qi-invigorating and blood-nourishing compound composition, comprising the following steps: mixing red ginseng powder, mulberry powder, wolfberry powder, tremella polysaccharide, blood protein polypeptide and ferrous gluconate to obtain the Qi-invigorating and blood-nourishing compound composition.

[0027] The compound composition with qi-tonifying and blood-nourishing properties is an oral preparation, which may be an oral solution, emulsion, powder, tablet, or granule.

[0028] The Qi-nourishing and blood-tonifying compound composition also includes excipients that can be used in pharmaceuticals and food. The excipients are selected from one or more of the following: sweeteners, acid regulators, fillers, flavoring agents, colorants, antioxidants, thickeners, stabilizers, emulsifiers, anti-caking agents, flow aids, and lubricants.

[0029] This invention also relates to the application of the above-mentioned Qi-invigorating and blood-nourishing compound composition in the preparation of Qi-invigorating and blood-nourishing products.

[0030] Compared with the prior art, the present invention has the following advantages:

[0031] (1) This invention promotes the effects of replenishing qi and blood and relieving fatigue through the scientific ratio of red ginseng powder, wolfberry powder, mulberry powder, tremella polysaccharide, blood protein polypeptide and ferrous gluconate. It can replenish qi and blood, nourish yin and improve the sub-health state of qi and blood deficiency.

[0032] (2) The red ginseng powder in the composition of the present invention is extracted by step temperature change, which improves the yield of red ginseng extract and the dissolution rate of total ginsenosides. Compared with red ginseng powder extracted by ordinary process, the red ginseng powder obtained by this process upgrade shows more significant effects in replenishing blood and qi.

[0033] (3) The tremella polysaccharide in the composition of the present invention is extracted by a compound enzymatic hydrolysis method. The tremella polysaccharide obtained after this process upgrade shows a more significant effect in nourishing blood and replenishing qi.

[0034] (4) The Qi-tonifying and blood-nourishing compound prepared by the present invention has clear components and efficacy, wide applicability, few side effects, simple preparation operation, low cost, wide applicable dosage forms, and is easy to produce and apply.

[0035] (5) Compared with the traditional Chinese medicine formulas which are troublesome to decoct and have poor effects, the active ingredients in the composition of the present invention have stable structures and high bioavailability. Attached Figure Description

[0036] Figure 1 These are typical images showing the staining intensity of red blood cells in the hearts of zebrafish after different treatment groups.

[0037] Figure 2 This is a graph showing the effect of different treatment groups on improving anemia in zebrafish.

[0038] Figure 3 These are typical images of zebrafish movement trajectories after different treatment groups; the black line represents the distance of slow movement, the green line represents the distance of medium movement, and the red line represents the distance of fast movement.

[0039] Figure 4 This is a graph showing the effect of different treatment groups on the movement ability of fatigued zebrafish.

[0040] Figure 2 and Figure 4 In comparison with the model control group, P<0.0001, P < 0.001, P < 0.01, P<0.05; compared with Example 1 group, #### P<0.0001, ### P<0.001, ## P<0.01, # P<0.05. Detailed Implementation

[0041] The present invention will be further described in detail below with reference to specific embodiments. The following embodiments are not intended to limit the present invention, but only to illustrate the present invention. Unless otherwise specified, the experimental methods used in the following embodiments are generally performed under conventional conditions. Unless otherwise specified, the materials and reagents used in the following embodiments are commercially available.

[0042] Mulberry powder was purchased from Tianjin Zhenruoguo Food Industry Co., Ltd.; wolfberry powder was purchased from Tianjin Jincheng Biotechnology Co., Ltd.; ferrous gluconate was purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.; and hemoprotein polypeptide was purchased from Shanghai Zhaoming Industrial Co., Ltd.

[0043] Example 1

[0044] A compound formula for invigorating qi and nourishing blood, comprising the following ingredients by weight: 12 parts red ginseng powder, 17 parts wolfberry powder, 12 parts mulberry powder, 13 parts tremella polysaccharide, 13 parts blood protein polypeptide and 1.4 parts ferrous gluconate.

[0045] The preparation method of the above-mentioned Qi-tonifying and blood-nourishing compound composition is as follows:

[0046] (1) Preparation of red ginseng powder:

[0047] S1. Place 100kg of sliced ​​red ginseng into a mixing tank, add purified water at a weight ratio of red ginseng to water of 1:10, and use a stepped temperature extraction method (extract at 60℃ for 1 hour, then at 90℃ for 2 hours, and then at 60℃ for 1 hour). After extraction, pass the filtrate through a 300-mesh sieve to obtain red ginseng extract.

[0048] S2. The red ginseng extract was transferred to a concentrator at 55°C and concentrated to a Brix value of 68%, yielding 70.0 kg of red ginseng extract with a yield of 45 wt%. The total ginsenoside content in the red ginseng extract was determined to be 2.4% using the detection method in Appendix B of GB 19506-2009.

[0049] S3. Freeze-dry the red ginseng extract, pulverize it, and obtain red ginseng powder.

[0050] (2) Preparation of Tremella polysaccharide:

[0051] a. Raw material pretreatment: Pulverized white fungus, passed through a 50-mesh sieve, and mixed with sodium phosphate buffer (0.05M, pH5.8) at a material-to-liquid ratio of 1g:30mL. Stirred at 52℃ for 30min to obtain a mixed solution.

[0052] b. Compound enzymatic hydrolysis: Add chitinase and β-glucanase in a mass ratio of 1:1.5 to the mixed solution, with the amount added being 2% of the mass of the dried tremella powder, and react in a constant temperature shaker at 180 rpm for 3 hours.

[0053] c. Enzyme inactivation and centrifugation: After the enzymatic hydrolysis reaction is completed, the enzyme is inactivated (90℃, 15min). The inactivated reaction solution is cooled to room temperature and centrifuged at 4500 rpm for 15min. The supernatant is collected.

[0054] d. Concentration and alcohol precipitation: The supernatant was concentrated to 1 / 4 of its original volume by rotary evaporation under reduced pressure at 60°C to obtain a concentrated solution. Anhydrous ethanol was slowly added to the concentrated solution while stirring, until the final volume fraction of ethanol reached 75%. The solution was then left to stand overnight at 4°C to allow the polysaccharides to precipitate fully.

[0055] e. Washing and drying: The precipitate is washed twice each with anhydrous ethanol and acetone to remove moisture, pigments, and small molecule impurities. The washed precipitate is then dried in a vacuum drying oven at 55℃ and pulverized to obtain Tremella polysaccharide.

[0056] (3) Mix red ginseng powder, wolfberry powder, mulberry powder, tremella polysaccharide, blood protein polypeptide and ferrous gluconate to obtain the Qi-nourishing and blood-tonifying compound composition.

[0057] Example 2

[0058] A compound formula for invigorating qi and nourishing blood, comprising the following ingredients by weight: 10 parts red ginseng powder, 5 parts wolfberry powder, 5 parts mulberry powder, 5 parts tremella polysaccharide, 15 parts blood protein polypeptide and 0.5 parts ferrous gluconate.

[0059] The preparation method of the above-mentioned Qi-tonifying and blood-nourishing compound composition is the same as that in Example 1.

[0060] Example 3

[0061] A compound formula for invigorating qi and nourishing blood, comprising the following ingredients by weight: 20 parts red ginseng powder, 20 parts wolfberry powder, 20 parts mulberry powder, 15 parts tremella polysaccharide, 10 parts blood protein polypeptide, and 2 parts ferrous gluconate.

[0062] The preparation method of the above-mentioned Qi-tonifying and blood-nourishing compound composition is the same as that in Example 1.

[0063] Comparative Examples 1-6

[0064] The only difference between Comparative Examples 1-6 and Example 1 is the weight percentage of the raw materials, as shown in Table 1.

[0065] Table 1. Raw material weight parts

[0066]

[0067] Comparative Example 7

[0068] The only difference between this comparative example and Example 1 is the preparation method of the red ginseng powder, as detailed below.

[0069] Preparation of red ginseng powder:

[0070] S1. Place 100kg of sliced ​​red ginseng into a mixing tank, add purified water at a weight ratio of red ginseng to water of 1:10, extract at 90℃ for 2 hours, repeat the extraction twice, and after extraction, pass the filtrate through a 300-mesh sieve to obtain red ginseng extract.

[0071] S2. The red ginseng extract was transferred to a concentrator at 55°C and concentrated to a Brix value of 68%, yielding 68.6 kg of red ginseng extract with a yield of 42 wt%. The total ginsenoside content in the red ginseng extract was determined to be 2.0% using the detection method in Appendix B of GB 19506-2009.

[0072] S3. Freeze-dry the red ginseng extract, pulverize it, and obtain red ginseng powder.

[0073] Comparative Example 8

[0074] The only difference between this comparative example and Example 1 is the preparation method of the Tremella fuciformis polysaccharide. Specifically:

[0075] In step b, only 2% of the weight of the dried tremella powder is added as chitinase during the enzymatic hydrolysis process.

[0076] Comparative Example 9

[0077] The only difference between this comparative example and Example 1 is the preparation method of the Tremella fuciformis polysaccharide. Specifically:

[0078] In step b, only 2% of the weight of the dried tremella powder is added to the enzymatic hydrolysis process with β-glucanase.

[0079] Effect test

[0080] 1. Test of Qi and Blood Tonifying Effect

[0081] Normally developed 3dpf wild-type AB strain zebrafish were randomly selected and placed in six-well plates, with 30 zebrafish treated in each well. The compositions of each example and comparative example were administered in water (10 mg of each composition was dissolved in 10 mL of water for each group). A positive control group was given at a concentration of 250 μg / mL of Shengxueninging, and blank and model control groups were also set up, with a volume of 3 mL per well. After incubation at 28℃ for 6 hours, except for the blank control group, 0.5 μg / mL of phenylhydrazine was added to each experimental group to establish a zebrafish anemia model. After incubation at 28℃ for 18 hours, the zebrafish were stained with o-anisidine.

[0082] After staining, 10 zebrafish were randomly selected from each experimental group, fixed with 3% methylcellulose, observed and photographed under a stereomicroscope, and the data were analyzed and collected using Image Pro Plus 6.0 advanced image processing software. The staining intensity of zebrafish cardiac erythrocytes was analyzed, and the statistical significance of cardiac erythrocyte staining intensity was used to evaluate the improvement effect of the samples on anemia. The cardiac erythrocyte staining intensity of each group is shown in Table 2. Figure 1 and Figure 2 As shown.

[0083] Table 2. Effects of different treatment groups on the improvement of anemia in zebrafish

[0084]

[0085] Note: Compared with the model control group, P<0.0001, P < 0.001, P < 0.01, P<0.05; compared with Example 1, #### P<0.0001, ### P<0.001, ## P<0.01, # P<0.05.

[0086] A zebrafish anemia model was established by phenylhydrazine induction, as shown in Table 2. Figure 1 and Figure 2 The results showed that the staining intensity of red blood cells in the heart of zebrafish in the model control group was significantly lower than that in the blank control group (P < 0.0001), indicating that the model was successfully established.

[0087] The results showed that the staining intensity of zebrafish heart erythrocytes in the treatment groups of Example 1, Example 2 and Example 3 was significantly increased compared with the model control group (P < 0.0001), with the best effect in Example 1.

[0088] From Table 2 and Figure 2 It can be seen that there are significant differences between Comparative Examples 1-6 and Example 1, indicating that the scientific formulation of the composition of the present invention has a significant synergistic effect on improving anemia in zebrafish caused by phenylhydrazine.

[0089] There was no significant difference between the 7 comparative groups and the model control group, but there was a significant difference compared with Example 1 (P < 0.0001), indicating that the red ginseng powder extracted by stepwise temperature variation in the composition of the present invention has excellent and significant effects on improving anemia in zebrafish.

[0090] Although comparative examples 8 and 9 showed significant differences compared with the model control group (P < 0.001), they also showed significant differences compared with Example 1. This indicates that the Tremella polysaccharide extracted by the compound enzymatic hydrolysis method in the composition of the present invention has a more significant effect on improving anemia in zebrafish than the Tremella polysaccharide extracted by only one of the single enzymatic hydrolysis methods.

[0091] 2. Anti-fatigue efficacy test

[0092] Wild-type AB strain zebrafish with normal development and a 4-day post-flop (dpf) were randomly selected and placed into six-well plates, with 30 fish per well. The compositions of each example and comparative example were administered in water (10 mg of each composition was dissolved in 10 mL of water for each group). A positive control of 1000 μg / mL of Zhonghua Dieda Wan (a traditional Chinese medicine for traumatic injury) was provided. A blank control group and a model control group were also set up, with a volume of 3 mL per well. After incubation at 28℃ for 24 hours, except for the blank control group, all other experimental groups were administered 8 mg / mL of anhydrous sodium sulfite to induce a zebrafish fatigue model.

[0093] After the test samples were treated with anhydrous sodium sulfite for 20 min, 10 zebrafish were randomly selected from each experimental group and transferred to a 48-well plate, one zebrafish per well. Data were collected using a behavior analyzer, and the total distance of the zebrafish movement was analyzed. The statistical significance of this index was used to evaluate the efficacy of the sample in relieving physical fatigue. The total distance of the zebrafish movement in each group is shown in Table 3. Figure 3 and Figure 4 As shown.

[0094]

[0095] Note: Compared with the model control group, P<0.0001, P < 0.001, P < 0.01, P<0.05; compared with Example 1, #### P<0.0001, ### P<0.001, ## P<0.01, # P<0.05.

[0096] Sodium sulfite reacts with dissolved oxygen in water, creating an oxygen-deficient environment, thereby inducing hypoxic fatigue in zebrafish. (Table 3...) Figure 3 and Figure 4 It can be seen that the total movement distance of the zebrafish in the model control group was significantly reduced compared with that of the blank control group, indicating that the fatigue zebrafish model was successfully constructed.

[0097] The results showed that the total movement distance of zebrafish in the treatment groups of Example 1, Example 2 and Example 3 was significantly increased compared with the model control group (P < 0.0001), with the best effect in Example 1.

[0098] From Table 3 and Figure 4 It can be seen that there are significant differences between Comparative Examples 1-6 and Example 1, indicating that the scientific formulation of the composition of the present invention can synergistically promote the anti-fatigue ability of zebrafish.

[0099] Compared with the model control group, the 7 comparative groups showed significant differences (P < 0.01), but compared with the 1 example group, there were still significant differences (P < 0.001), indicating that the red ginseng powder extracted by step-temperature variation in the composition of the present invention is excellent and significant in improving the mobility of fatigued zebrafish.

[0100] Although comparative examples 8 and 9 showed significant differences compared to the model control group, they also showed significant differences compared to Example 1. The tremella polysaccharide extracted by the compound enzymatic hydrolysis method in the composition of the present invention has a more significant effect on the anti-fatigue ability of zebrafish than the tremella polysaccharide extracted by only one of the single enzymatic hydrolysis methods.

[0101] The above detailed description is a specific description of one of the feasible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. All equivalent implementations or modifications that do not depart from the present invention should be included within the scope of the technical solution of the present invention.

Claims

1. A Qi and blood nourishing compound composition, characterized in that, The raw materials of the Yiqi Yangxue compound composition include, by weight fraction, 10-20 parts of red ginseng powder, 5-20 parts of mulberry powder, 5-20 parts of Chinese wolfberry powder, 5-15 parts of tremella polysaccharide, 5-15 parts of blood protein polypeptide, and 0.5-2 parts of ferrous gluconate; The preparation method of the red ginseng powder includes the following steps: mixing red ginseng slices with water, extracting by using a stepwise variable temperature, filtering, concentrating, drying, and crushing to obtain the red ginseng powder. The weight ratio of the red ginseng slices to water is 1:8-12; the stepwise variable temperature is 55-65℃ for 0.8-1.2h, 85-95℃ for 1.5-2.5h, and 55-65℃ for 0.8-1.2h; and the concentration temperature is 50-60℃, and the concentration is performed until the sugar content of the red ginseng extract is ≥60%.

2. The Qi and blood nourishing compound composition according to claim 1, characterized in that, The raw materials of the Yiqi Yangxue compound composition include, by weight fraction, 10-15 parts of red ginseng powder, 10-15 parts of mulberry powder, 15-20 parts of Chinese wolfberry powder, 10-15 parts of tremella polysaccharide, 10-15 parts of blood protein polypeptide, and 1-2 parts of ferrous gluconate.

3. The Qi and blood nourishing compound composition according to any one of claims 1-2, characterized in that, The molecular weight of the blood protein polypeptide is <1000 Da.

4. The Qi and blood nourishing compound composition according to any one of claims 1-2, characterized in that, The preparation method of the tremella polysaccharide includes the following steps: crushing and sieving tremella, mixing with a phosphate buffer, stirring, adding a compound enzyme for enzymolysis, centrifuging, concentrating the supernatant, alcohol precipitation, washing, and drying.

5. The Qi and blood nourishing compound composition according to claim 4, characterized in that, The solid-liquid ratio of the tremella to the phosphate buffer is 1g:20-40mL, the phosphate buffer is a sodium phosphate buffer with a concentration of 0.01-0.1M and a pH of 5.5-6.5, the stirring temperature is 50-55℃, and the stirring time is 20-30min; the compound enzyme is chitinase and β-glucanase with a mass ratio of 1:1-2, the enzymolysis temperature is 50-55℃, the pH is 5.5-6.0, the enzymolysis process is oscillated at a speed of 150-200 rpm in a constant-temperature shaker for 2-4h, and the addition amount of the compound enzyme is 1%-3% of the mass of the tremella dry powder.

6. A method for preparing the Yiqi Yangxue compound composition according to any one of claims 1-5, characterized in that, The following steps are included: Mixing the red ginseng powder, the mulberry powder, the Chinese wolfberry powder, the tremella polysaccharide, the blood protein polypeptide, and the ferrous gluconate to obtain the Yiqi Yangxue compound composition.

7. The production method according to claim 6, characterized by, The Yiqi Yangxue compound composition further includes a supplementary material that can be used in drugs and food, and the supplementary material is selected from one or more of an acid regulator, a filler, a flavoring agent, a coloring agent, an antioxidant, a thickening agent, a stabilizer, an emulsifier, an anti-caking agent, a flow aid, and a lubricant.

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