Kit

Abstract

The present invention provides a kit, which comprises: a main shell, which is internally provided with an adjusting channel penetrating in the horizontal direction, a plurality of input channels are arranged above the adjusting channel, and a plurality of output channels are arranged below the adjusting channel; the adjusting valve is arranged in the adjusting channel and can reciprocate along the adjusting channel; a plurality of conveying channels are arranged on the adjusting valve; and when the adjusting valve is configured to move to a specific position, the corresponding conveying channel can communicate two input channels, or one input channel can communicate with one output channel. The kit provided by the invention can realize integrated totally-closed detection, improve the detection efficiency and prevent reagents from being polluted.

Description

Technical Field

[0001] This application belongs to the field of biological and chemical detection technology, and in particular relates to a reagent kit. Background Technology

[0002] In the fields of biological and chemical detection, reagent kits are commonly used to hold various analytes. For example, in nucleic acid detection, common steps include: first, pre-treating the cells in the analyte by lysis to obtain the target nucleic acid sequence; then, amplifying the sequence to obtain a large quantity of the target nucleic acid; and finally, detecting the large quantity of the target nucleic acid sequence, such as by fluorescence signal detection, to improve detection accuracy. All of these steps require lysing the sample and amplifying the target nucleic acid sequence in different tubes or reagent kits for detection. In the field of chemical detection, frequent container switching during analyte testing can lead to reagent contamination and low operational efficiency.

[0003] In existing technologies, reagent kits typically consist of a single chamber. In practical testing, a common method is to first pre-treat the analyte separately, such as by lysis, and then transfer the resulting target nucleic acid sequence into the reagent kit for amplification and detection. This reagent transfer process is not only inefficient but also prone to contamination. Summary of the Invention

[0004] The purpose of this invention is to solve the technical problems of low efficiency and susceptibility to contamination in nucleic acid testing. This invention provides a reagent kit that enables integrated, fully enclosed testing, improving testing efficiency and preventing reagent contamination.

[0005] To address the aforementioned technical problems, embodiments of the present invention provide a reagent kit, including a regulating valve. Above the regulating valve are horizontally spaced lysis channels, quantitative channels, and storage channels, and below the regulating valve is an amplification tube. The lysis channels are used to collect and lyse the analyte; the quantitative channels are used to measure a preset volume of the analyte and transfer it to the amplification tube; the storage channels are used to store and deliver liquid reagents to the amplification tube; and the amplification tube is used to amplify and culture the analyte or provide reaction conditions. The regulating valve can reciprocate in the horizontal direction. When the regulating valve is configured to move to a specific position, it can connect two of the pyrolysis channel, the metering channel, the reservoir channel, and the amplification tube.

[0006] In an optional embodiment of this application, the regulating valve is provided with a pipetting channel, an injection channel, and a replenishment channel arranged at intervals. When the regulating valve is moved to the first position, one end of the pipetting channel is connected to the lysis channel and the other end is connected to the quantitative channel. When the regulating valve is moved to the second position, one end of the injection channel is connected to the quantitative channel and the other end is connected to the amplification tube. When the regulating valve is moved to the third position, one end of the replenishment channel is connected to the storage channel and the other end is connected to the amplification tube.

[0007] In an optional embodiment of this application, the pyrolysis channel includes a pressurization section and a pyrolysis section arranged sequentially from top to bottom; the pressurization section is used to increase the gas pressure in the pyrolysis section; the sidewall structure of the pyrolysis section is designed to allow the material in the pyrolysis section to exchange heat with the outside world, so as to control the temperature of the analyte in the pyrolysis section.

[0008] In an optional embodiment of this application, the quantitative channel includes a quantitative tube and a waste liquid tube arranged side by side in a horizontal direction; the volume of the quantitative tube is equal to the preset volume, and the upper end of the quantitative tube is connected to the waste liquid tube; when the regulating valve moves to the first position, the lower end of the quantitative tube is connected to the transfer channel; when the regulating valve moves to the second position, the lower end of the quantitative tube is connected to the injection channel.

[0009] In an optional embodiment of this application, the metering tube is positioned close to the side wall of the reagent kit. The arrangement of the metering tube close to the side wall allows the liquid inside the metering tube to exchange heat with the outside environment, thereby controlling the temperature of the liquid inside the metering tube.

[0010] In an optional embodiment of this application, the liquid storage channel includes a pressure zone and a liquid storage tube arranged sequentially in a vertical direction. The pressure zone is used to increase the gas pressure inside the liquid storage tube, and the liquid reagent is stored inside the liquid storage tube.

[0011] In an optional embodiment of this application, the liquid reservoir is positioned close to the side wall of the reagent kit, and the side wall of the reagent kit close to the liquid reservoir is configured to allow the liquid reagent in the liquid reservoir to exchange heat with the outside environment in order to control the temperature of the liquid reagent.

[0012] In an optional embodiment of this application, a first output channel and a second output channel are constructed between the regulating valve and the amplification tube. When the regulating valve moves to the second position, the two ends of the first output channel are connected to the injection channel and the amplification tube, respectively. When the regulating valve moves to the third position, the two ends of the second output channel are connected to the replenishment channel and the amplification tube, respectively.

[0013] In an optional embodiment of this application, a storage device is provided between the amplification tube and the second output channel, and the storage device contains a storage space that communicates with the second output channel and the amplification tube.

[0014] In an optional embodiment of this application, a first lyophilized reagent is stored in the amplification tube, and a second lyophilized reagent is stored in the replenishment channel and / or storage space. The liquid reagent is used to dissolve the second lyophilized reagent and / or react with the second lyophilized reagent.

[0015] Compared with existing technologies, the reagent kit and control method provided in this application have at least the following beneficial effects: The reagent kit provided by the embodiments of the present invention features an adjustment channel within the main housing, with an input channel above the adjustment channel and an output channel below it. Moving the position of the adjustment valve within the adjustment channel allows for connection between two input channels or between one input channel and one output channel. This enables pipetting, reagent mixing, and reagent dispensing. Compared to the cumbersome operations of multiple reagent transfers in existing reagent kits using multiple single chambers, the reagent kit provided by the present invention integrates multiple chambers within a single main housing, allowing for selective connection between the chambers, thus improving the overall integration of the reagent kit. This reduces experimental steps, improves biological detection efficiency, reduces reagent contact time with the external environment, and avoids reagent contamination caused by reagent transfer. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application; those skilled in the art can obtain other drawings based on these drawings without any creative effort.

[0017] Figure 1 A schematic diagram of the overall structure of a reagent kit provided in an embodiment of the present invention is shown; Figure 2 A cross-sectional view of a kit provided according to an embodiment of the present invention is shown to illustrate the lysis channel, the injection channel, and the quantification channel; Figure 3 Shown Figure 2 A magnified view of part A in the middle to show the internal communication pathway a of the reagent kit when the regulating valve is in the first position; Figure 4 A cross-sectional view of a reagent kit provided in another embodiment of the present invention is shown to illustrate the quantitative channel, the pipetting channel, and the first output channel; Figure 5 Shown Figure 4 A magnified view of part B in the middle section to show the internal communication pathway b of the reagent kit when the regulating valve is in the second position; Figure 6 A cross-sectional view of a reagent kit provided in another embodiment of the present invention is shown to illustrate the reservoir channel, the replenishment channel, and the second output channel; Figure 7 Shown Figure 6 A magnified view of section C in the middle to show the internal connectivity pathway c of the reagent kit when the regulating valve is in the third position.

[0018] The accompanying figure is labeled as follows: 1. Main shell, 2. Connecting column, 3. Amplification tube, 4. Storage device, 5. Storage space, 6. Regulating valve, 7. Delivery channel, 8. Pipette channel, 9. Injection channel, 10. Replenishment channel, 11. Regulating channel, 12. Input channel, 13. Pyrolysis channel, 14. Pressurization section, 15. Pyrolysis section, 16. Feed piston, 17. Feed port, 18. Piston cap, 19. Metering channel, 20. Metering tube, 21. Waste liquid tube, 22. Sealing cap, 23. Storage channel, 24. Replenishment piston, 25. Piston clamp, 26. Pressure zone, 27. Storage tube, 28. Output channel, 29. First output channel, 30. Second output channel, 31. Extension tube. Detailed Implementation

[0019] To make the above and other features and advantages of this application clearer, the invention is further described below with reference to the accompanying drawings. It should be understood that the specific embodiments given herein are for the purpose of explanation to those skilled in the art and are exemplary only, not restrictive.

[0020] In the description of this application, features specified with "first" or "second" are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Features specified with "first" or "second" may explicitly or implicitly include at least one of the specified features. The use of the term "multiple" generally means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0021] In this application, unless otherwise explicitly specified and limited, terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can be a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0022] In the description of this application, the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that the specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0023] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings. Please refer to... Figures 1 to 6 , Figure 1 A schematic diagram of the overall structure of a reagent kit provided in an embodiment of the present invention is shown. Figure 2 A cross-sectional view of a kit provided in an embodiment of the present invention is shown to illustrate the lysis channel 13, the injection channel 9, and the quantification channel 19. Figure 3 Shown Figure 2 A magnified view of part A in the middle to show the internal communication pathway a of the reagent kit when the regulating valve is in the first position. Figure 4 A cross-sectional view of a kit provided in another embodiment of the present invention is shown to illustrate the quantitative channel 19, the pipetting channel 8, and the first output channel 29. Figure 5 Shown Figure 4 A magnified view of part B in the diagram to show the internal communication pathway b of the reagent kit when the regulating valve is in the second position. Figure 6 A cross-sectional view of a reagent kit provided in another embodiment of the present invention is shown to illustrate the reservoir channel 23, the replenishment channel 10, and the second output channel 30. Figure 7 Shown Figure 6 A magnified view of section C in the middle to show the internal connectivity pathway c of the reagent kit when the regulating valve is in the third position.

[0024] To address the aforementioned technical problems, embodiments of the present invention provide a reagent kit comprising a main housing 1 and a regulating valve 6. The main housing 1 has a horizontally extending regulating channel 11 internally. Multiple input channels 12 are located above the regulating channel 11, and multiple output channels 28 are located below the regulating channel 11. The regulating valve 6 is disposed within the regulating channel 11 and is capable of reciprocating along the regulating channel 11. The regulating valve 6 has multiple delivery channels 7. When the regulating valve 6 is configured to move to a specific position, the corresponding delivery channel 7 can connect two of the input channels 12, or connect one of the input channels 12 to one of the output channels 28.

[0025] By adopting the above technical solution, an adjustment channel 11 is set inside the main housing 1, an input channel 12 is set above the adjustment channel 11, and an output channel 28 is set below the adjustment channel 11. Moving the position of the adjustment valve 6 within the adjustment channel 11 allows for connection between two input channels 12, or connection between one input channel 12 and one output channel 28. This enables pipetting, reagent mixing, and reagent output in a fully enclosed environment. Compared to the cumbersome operation of multiple reagent transfers in existing reagent kits using multiple single cavities, the reagent kit provided by this invention integrates multiple cavities within the same main housing 1, allowing selective connection between the cavities, thus improving the overall integration of the reagent kit. This reduces experimental steps, improves biological detection efficiency, reduces reagent contact time with the external environment, and avoids reagent contamination caused by reagent transfer.

[0026] It is worth mentioning that the kit provided by this invention can be used for nucleic acid detection, but its use in other biological detection, biological experiments, and chemical experiments is not excluded. Any kit using the same or substantially the same structure as the kit provided by this invention, whether used for reagent quantification, reagent mixing, reagent transfer, or reagent culture, is within the scope of protection of this application. In some large-scale biological or chemical detections, multiple reaction reagents or culture reagents are included. Different reagents can be stored in multiple input channels 12, or various reagents can be quantified and processed separately. The number of input channels 12 and output channels 28 is not limited here; those skilled in the art can set the number of input channels 12 and output channels 28 according to the number of reagents and processing steps in the biological or chemical detection.

[0027] Further, if Figure 4 and Figure 6 As shown, input channel 12 includes channels along the horizontal direction (specifically, the width direction of the reagent kit, such as...). Figures 1 to 6 The pyrolysis channel 13, the metering channel 19, and the storage channel 23 are arranged at intervals (as shown in the Y direction). Figure 6As shown. In some embodiments, the lysis channel 13 is used to receive and lyse the analyte; the quantification channel 19 is used to measure a preset volume of the analyte and transfer it to the output channel 28; the reservoir channel 23 is used to store and deliver liquid reagents to the output channel 28. Specifically, the liquid reagent can be a reagent with the same solvent as the analyte, a reagent that reacts biochemically with the analyte, or a buffer solution or pure water. The operator can add the corresponding liquid reagent to the reservoir channel 23 according to experimental needs. It should be noted that there can be multiple reservoir channels 23, and multiple reservoir channels 23 can store different types and functions of liquid reagents respectively, and multiple liquid reagents can be used in different steps in the same biochemical experiment. In other embodiments, the lysis channel 13 can be used for processing steps such as buffering, heat treatment, and biological culture of the analyte, and the reservoir channel 23 can also be used to store reagents and / or process reagents (such as heating, light irradiation, etc.). Specifically, the analyte described in this invention is a flowable fluid analyte, which may be an aqueous solution or suspension of the analyte, or a substance that can be mixed with other reagents to form a fluid within the kit provided in this application. That is, the analyte needs to be able to flow within the input channel 12, the delivery channel 7, and the output channel 28.

[0028] Further, if Figure 2 and Figure 4 As shown, the lysis channel 13 includes a pressurization section 14 and a lysis section 15 arranged sequentially from top to bottom. A feed piston 16 is provided within the pressurization section 14. Specifically, the cross-sections (i.e., the horizontal cross-sections, the same below) of the pressurization section 14 and the feed piston 16 are circular, and the cross-sectional area of ​​the pressurization section 14 is smaller than that of the lysis section 15. A step is formed between the pressurization section 14 and the lysis section 15, which limits the minimum downward movement height of the feed piston 16. The bottom surface of the lysis section 15 has an output port for connecting to the delivery channel 7. The feed piston 16 is used to increase the air pressure within the lysis channel 13 to inject the analyte from the output port into the delivery channel 7. In some embodiments, the analyte is a biological cell or biological cell metabolite containing a target nucleic acid sequence. The sidewall structure of the lysis section 15 is designed to transfer heat into the lysis channel 13 to heat the analyte, causing it to lyse and release the target nucleic acid sequence. Preferably, the main shell 1 can be integrally processed from a thermally conductive material to facilitate heat transfer. To save materials, the main shell 1 can also be processed in sections, with thermally conductive materials used near the sidewalls of the pyrolysis section 15. In other embodiments, the analyte can be a solution or suspension of biological or synthetic materials, or a solution or suspension of ore or alloy powder. The pyrolysis section 15 can be used for pretreatment of the analyte, such as pyrolysis, dissolution, and decomposition, to prepare it for the next stage of the experiment.

[0029] Further, if Figures 1 to 6As shown, the feed piston 16 is constructed with a vertically oriented ( Figures 1 to 6 A through-hole 17 (shown in the X direction) is provided for adding the analyte into the lysis channel 13. A piston cap 18 is provided at the upper end of the inlet 17 to seal it. Specifically, when the kit is not in use, the piston cap 18 tightly covers the inlet 17 to ensure the internal space of the kit is isolated from the outside environment, preventing contamination. When it is necessary to add the analyte to the kit, the piston cap 18 is opened, and the analyte is quickly injected into the inlet 17. The piston cap 18 is then returned to its sealed position to prevent contamination of the kit. Furthermore, when the piston pushes downwards to inject the analyte into the delivery channel 7, the piston cap 18 prevents depressurization of the lysis channel 13, ensuring an increase in pressure within the lysis channel 13 for downward output of the analyte.

[0030] Further, if Figure 6 As shown, the delivery channel 7 includes a pipetting channel 8, an injection channel 9, and a replenishment channel 10 arranged at intervals. When the regulating valve 6 moves to the first position, one end of the pipetting channel 8 is connected to the pyrolysis channel 13, and the other end is connected to the metering channel 19, as shown. Figure 3 As shown in the middle passage a. When the regulating valve 6 moves to the first position, the feed piston 16 moves downward, increasing the air pressure inside the pyrolysis channel 13. This allows the analyte inside the pyrolysis channel 13 to be injected from the output port at the lower end of the pyrolysis channel 13 into the pipetting channel 8, and then along the pipetting channel 8 into the metering channel 19 to measure a preset volume of the analyte. When the regulating valve 6 moves to the second position, one end of the injection channel 9 is connected to the metering tube 20, and the other end is connected to the output channel 28, as shown in the middle passage a. Figure 5 As shown in the middle passage (b), when the feed piston 16 injects the analyte into the quantitative channel 19, it increases the air pressure within the quantitative channel 19. When the quantitative tube 20 is connected to the output channel 28 through the injection channel 9, the air pressure in the quantitative channel 19 is greater than the air pressure in the output channel 28. Therefore, the analyte in the quantitative channel 19 can automatically flow into the output channel 28 under the action of the air pressure difference, realizing the transfer of the preset volume of analyte reagent. When the regulating valve 6 moves to the third position, one end of the replenishment channel 10 is connected to the storage channel 23, and the other end is connected to the output channel 28, as shown in the middle passage (b). Figure 7 The middle pathway (c) is shown. This facilitates the mixing of the liquid reagent with the aforementioned preset volume of the analyte.

[0031] Further, if Figure 2 and Figure 4As shown, the quantitative channel 19 includes a quantitative tube 20 and a waste liquid tube 21 arranged side by side in a horizontal direction. The volume of the quantitative tube 20 is equal to the preset volume of the analyte, and the upper end of the quantitative tube 20 is connected to the waste liquid tube 21. When the regulating valve 6 moves to the first position, the lower end of the quantitative tube 20 is connected to the pipetting channel 8. The analyte output from the pipetting channel 8 first enters the quantitative tube 20. After the quantitative tube 20 is full, the analyte overflows from the top of the quantitative tube 20 into the waste liquid tube 21. When the quantitative tube 20 is full, the preset volume of the analyte is measured. At the same time, the excess analyte liquid overflows into the waste liquid tube 21 and separates from the analyte liquid in the quantitative tube 20. When the liquid in the quantitative tube 20 flows out from the lower end of the quantitative tube 20, the gas in the quantitative channel 19 can enter the quantitative tube 20, while the excess analyte is retained in the waste liquid tube 21, ensuring that the volume of the analyte in the quantitative tube 20 remains unchanged. When the regulating valve 6 moves to the second position, the lower end of the metering tube 20 is connected to the injection channel 9, allowing the preset volume of analyte measured by the metering tube 20 to flow into the output channel 28, thus realizing the reagent transfer of the preset volume of analyte. Preferably, the metering tube 20 is located near the side wall of the metering channel 19, and the side wall of the metering channel 19 is constructed to transfer heat into the metering tube 20. Specifically, to facilitate heat transfer, the main housing 1 can be integrally machined using a thermally conductive material. To save material, the main housing 1 can also be machined in sections, with a thermally conductive material used near the side wall of the metering tube 20.

[0032] Furthermore, a replenishment piston 24 is provided within the reservoir channel 23. Below the replenishment piston 24 is a pressure zone 26, and below the pressure zone 26 is a reservoir tube 27, which stores buffer solution. Specifically, the cross-sections (i.e., the interface along the horizontal direction, the same below) of both the pressure zone 26 and the reservoir tube 27 are circular, and the cross-sectional area of ​​the reservoir tube 27 is smaller than that of the pressure zone 26. The bottom surface of the pressure zone 26 can limit the lowest position of the reservoir channel 23 when it moves downward. Setting the cross-sectional area of ​​the reservoir tube 27 to be smaller than that of the pressure zone 26 can increase the flow rate of the buffer solution output from the reservoir tube 27, facilitating the agitation of the buffer solution downstream for better mixing with downstream liquid or powdered reagents. When the regulating valve 6 moves to the third position, the reservoir tube 27 is connected to the replenishment channel 10; the replenishment piston 24 is used to increase the air pressure within the replenishment channel 10 to inject the liquid reagent from the reservoir channel 23 into the replenishment channel 10. Preferably, the liquid storage pipe 27 is disposed near the side wall of the main housing 1, and the side wall of the main housing 1 near the liquid storage pipe 27 is configured to transfer heat into the liquid storage pipe 27. Specifically, to facilitate heat transfer, the main housing 1 can be integrally machined using a thermally conductive material. To save material, the main housing 1 can also be machined in sections, with the side wall of the liquid storage pipe 27 being machined using a thermally conductive material. Similarly, for the input and output channels that require heating, the side walls can be machined using a thermally conductive material, including the entire main housing 1 and the side walls of the input and output channels.

[0033] Further, if Figure 4 and Figure 6 As shown, the output channel 28 includes a first output channel 29 and a second output channel 30, which are spaced apart and extend vertically. When the regulating valve 6 moves to the second position, the injection channel 9 is connected to the first output channel 29, allowing the preset volume of the analyte measured in the quantitative tube 20 to be transported downstream for incubation through the first output channel 29. When the regulating valve 6 moves to the third position, the replenishment channel 10 is connected to the second output channel 30, allowing the liquid reagent in the storage channel 23 to flow into the second output channel 30 through the replenishment channel 10, and then flow downstream through the second output channel 30 to mix with the analyte.

[0034] Further, if Figure 6As shown, the bottom of the main shell 1 is provided with a connecting post 2 extending vertically. The first output channel 29 and the second output channel 30 are constructed inside the connecting post 2 and pass through the connecting post 2. Below the connecting post 2 is an amplification tube 3 connected to the first output channel 29 and the second output channel 30. The amplification tube 3 is used to amplify the analyte. Specifically, the amplification tube 3 is made of transparent material, specifically a PCR tube (Polymerase Chain Reaction Tube, a special container used in molecular biology experiments to carry polymerase chain reactions). After a preset volume of analyte enters the amplification tube 3 through the first output channel 29, the reagents and analyte in the amplification tube 3 can be cultured and amplified according to the culture conditions. The culture conditions can be incubation, light irradiation, stirring, and the addition of liquid reagents, etc. In this example, the amplification tube 3 contains a first lyophilized reagent, and the replenishment channel 10 contains a second lyophilized reagent. When the liquid reagent passes through the replenishment channel 10, it can flush the second lyophilized reagent in the replenishment channel 10 and deliver the second lyophilized reagent into the amplification tube 3.

[0035] Furthermore, a storage component 4 is provided between the amplification tube 3 and the connecting post 2. The storage component 4 is sleeved on the connecting post 2, and the opening of the amplification tube 3 is sleeved on the storage component 4. An extension tube 31 is provided at the lower end of the connecting post 2, and the first output channel 29 passes through the extension tube 31 in a vertical direction. The storage component 4 has a storage space 5 and a clearance hole arranged sequentially and connected in a vertical direction inside. The extension tube 31 is inserted into the clearance hole, and the extension tube 31 and the clearance hole are sealed. The storage component 4 also has a third channel connected to the clearance hole. The upper end of the storage space 5 is connected to the second output channel 30, and the lower end is connected to the third channel. The lower end of the third channel is connected to the amplification tube 3. In this embodiment, the amplification tube 3 contains a first lyophilized reagent, and the storage space 5 can also store a second lyophilized reagent. When the liquid reagent passes through the storage space 5, it can flush the second lyophilized reagent into the amplification tube 3. Specifically, the choice of whether to store the second lyophilized reagent in the replenishment channel 10 or the storage space 5 depends on the physicochemical properties of the second lyophilized reagent, such as its solubility and flowability. When a large dose of the second lyophilized reagent is required, it can also be stored in both the replenishment channel 10 and the storage space 5.

[0036] Furthermore, the amplification tube 3 is equipped with a stirring device, which is used to rotate in an alternating magnetic field environment to stir the reagents inside the amplification tube 3. Specifically, the stirring device is a magnetic stir bar, which can vibrate inside the amplification tube 3 when the amplification tube 3 is placed in an alternating magnetic field to mix the various reagents inside the amplification tube 3.

[0037] Furthermore, a piston block 25 is provided above the liquid storage channel 23. The piston block 25 is detachably connected to the main housing 1 and is used to prevent the replenishment piston 24 from disengaging from the replenishment channel 10. The quantitative channel 19 has a drain port at its upper end, which is covered by a sealing cap 22. The piston block 25 has an extension, the lower surface of which presses against the upper surface of the sealing cap 22. After the biological detection is completed, the piston block 25 can be removed from the main housing 1, and the excess analyte in the quantitative channel 19 can be removed for recycling of the main housing 1.

[0038] According to another aspect of this application, embodiments of the present invention also provide a control method for the aforementioned reagent kit, the control method comprising: S1 injects the analyte into the lysis channel 13; S2 heats the sidewall of the pyrolysis channel 13 to pyrolyze the analyte; S3 moves the regulating valve 6 to the first position and moves the feed piston 16 downward to fill the metering tube 20 with the analyte. S4 moves the regulating valve 6 to the second position to deliver the analyte into the amplification tube 3; S5 Place the amplification tube 3 in an alternating magnetic field environment and stir the analyte and the first lyophilized reagent until well mixed. S6 places the amplification tube in a specific temperature environment to amplify and incubate the analyte inside the amplification tube for a specific time.

[0039] Furthermore, before heating the sidewall of the pyrolysis channel 13, the process also includes S11 sealing the pyrolysis channel 13, specifically by sealing the feed port 17 with the piston cap 18.

[0040] Furthermore, after amplification and culture for a specific time, the process also includes: S7 moving the regulating valve 6 to the third position, moving the liquid replenishment piston 24 downward, and driving the stirring device to stir. Additionally, the liquid inside the amplification tube 3 is detected under a specific temperature environment.

[0041] Furthermore, before injecting the analyte into the lysis channel 13, the method further includes S0 storing the second lyophilized reagent into the replenishment channel 10 and / or the storage space 5, and storing the first lyophilized reagent into the amplification tube 3.

[0042] While the present invention has been illustrated and described with reference to certain preferred embodiments, those skilled in the art should understand that the above description is a further detailed explanation of the invention in conjunction with specific embodiments, and should not be construed as limiting the specific implementation of the invention to these descriptions. Various changes in form and detail can be made by those skilled in the art, including several simple deductions or substitutions, without departing from the spirit and scope of the invention.

Claims

1. A reagent kit, characterized in that, Including regulating valves, Above the regulating valve are a lysis channel, a quantitative channel, and a storage channel arranged at intervals along the horizontal direction. Below the regulating valve is an amplification tube. The lysis channel is used to collect the analyte and lyse it; the quantitative channel is used to measure a preset volume of the analyte and transfer it into the amplification tube; the storage channel is used to store and deliver liquid reagents to the amplification tube; the amplification tube is used for amplification culture of the analyte or to provide reaction conditions. The regulating valve is capable of reciprocating in the horizontal direction. When the regulating valve is configured to move to a specific position, it can connect two of the lysis channel, the quantitative channel, the liquid storage channel, and the amplification tube.

2. The kit according to claim 1, characterized in that, The regulating valve is provided with a pipetting channel, an injection channel, and a replenishment channel arranged at intervals. When the regulating valve is moved to the first position, one end of the pipetting channel is connected to the lysis channel and the other end is connected to the quantitative channel. When the regulating valve is moved to the second position, one end of the injection channel is connected to the quantitative channel and the other end is connected to the amplification tube. When the regulating valve is moved to the third position, one end of the replenishment channel is connected to the storage channel and the other end is connected to the amplification tube.

3. The kit according to claim 2, characterized in that, The pyrolysis channel includes a pressurization section and a pyrolysis section arranged sequentially from top to bottom; the pressurization section is used to increase the gas pressure in the pyrolysis section; the sidewall of the pyrolysis section is constructed to allow the substances in the pyrolysis section to exchange heat with the outside world, so as to control the temperature of the analyte in the pyrolysis section.

4. The kit according to claim 2, characterized in that, The quantitative channel includes a quantitative tube and a waste liquid tube arranged side by side in a horizontal direction; the volume of the quantitative tube is equal to the preset volume, and the upper end of the quantitative tube is connected to the waste liquid tube; when the regulating valve moves to the first position, the lower end of the quantitative tube is connected to the liquid transfer channel; when the regulating valve moves to the second position, the lower end of the quantitative tube is connected to the liquid injection channel.

5. The kit according to claim 4, characterized in that, The metering tube is positioned close to the side wall of the reagent kit. The side wall of the metering tube is designed to allow the liquid inside the metering tube to exchange heat with the outside environment, thereby controlling the temperature of the liquid inside the metering tube.

6. The kit according to claim 2, characterized in that, The liquid storage channel includes a pressure zone and a liquid storage tube arranged sequentially in a vertical direction. The pressure zone is used to increase the gas pressure inside the liquid storage tube, and the liquid reagent is stored inside the liquid storage tube.

7. The kit according to claim 6, characterized in that, The reservoir tube is located near the side wall of the reagent kit, and the side wall of the reagent kit near the reservoir tube is configured to allow the liquid reagent in the reservoir tube to exchange heat with the outside environment in order to control the temperature of the liquid reagent.

8. The kit according to any one of claims 2 to 7, characterized in that, A first output channel and a second output channel are constructed between the regulating valve and the amplification tube. When the regulating valve moves to the second position, the two ends of the first output channel are connected to the injection channel and the amplification tube, respectively. When the regulating valve moves to the third position, the two ends of the second output channel are connected to the replenishment channel and the amplification tube, respectively.

9. The reagent kit as described in claim 8, characterized in that, A storage device is also provided between the amplification tube and the second output channel, and the storage device has a storage space that is connected to the second output channel and the amplification tube.

10. The kit according to claim 9, characterized in that, The amplification tube contains a first lyophilized reagent, and the replenishment channel and / or the storage space contains a second lyophilized reagent. The liquid reagent is used to dissolve the second lyophilized reagent and / or react with the second lyophilized reagent.

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