A composition containing an extract of physalis fruits and a method for its preparation

By scientifically combining prickly pear fruit extract, olive leaf extract, centella asiatica extract, and tremella polysaccharide, a complex polysaccharide system is formed, which solves the problems of poor stability and environmentally unfriendly extraction processes in existing cosmetics, and achieves highly effective antioxidant, soothing, and gentle skin care effects.

CN120859895BActive Publication Date: 2026-04-21GUANLI (HANGZHOU) BIOTECHNOLOGY CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANLI (HANGZHOU) BIOTECHNOLOGY CO LTD
Filing Date
2025-07-18
Publication Date
2026-04-21

AI Technical Summary

Technical Problem

Existing antioxidant and soothing cosmetics suffer from poor stability, strong irritation, and difficulty in breaking the vicious cycle of oxidation and inflammation. Furthermore, traditional extraction processes easily damage the active structure, failing to meet the multiple requirements of safety, gentleness, and high efficacy.

Method used

By using a scientific ratio of prickly pear fruit extract, olive leaf extract, centella asiatica extract and tremella polysaccharide, a complex polysaccharide system is formed through a specific extraction process, which enhances antioxidant and soothing properties, and produces green and environmentally friendly skin care products.

Benefits of technology

It achieves highly effective antioxidant, rapid soothing, and gentle, non-irritating skincare effects, meeting multiple skincare needs, and the extraction process is environmentally friendly and safe.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention discloses a composition containing prickly pear fruit extract and its preparation method, specifically belonging to the field of cosmetic raw material compounding. The composition consists of the following components in parts by weight: 10-20 parts prickly pear fruit extract, 1-3 parts olive leaf extract, 6-9 parts centella asiatica extract, and 0.5-0.8 parts tremella polysaccharide. This invention achieves a synergistic enhancement of antioxidant and soothing effects through the scientific ratio of prickly pear fruit extract, olive leaf extract, centella asiatica extract, and tremella polysaccharide, covering multiple skincare needs such as anti-aging and whitening, overcoming the limitations of single-ingredient efficacy.
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Description

Technical Field

[0001] This invention belongs to the field of cosmetic raw material compounding, specifically, it relates to a composition containing prickly pear fruit extract and its preparation method. Background Technology

[0002] On the one hand, ROS directly oxidizes lipids, proteins, and DNA, damaging the stratum corneum barrier and accelerating collagen fiber degradation; on the other hand, it activates signaling pathways such as NF-κB and AP-1, prompting the release of large amounts of pro-inflammatory mediators such as IL-1β, TNF-α, and COX-2, which manifest as skin stinging, erythema, dryness, itching, and desquamation, and may even induce chronic problems such as sensitivity, acne, and rosacea.

[0003] Currently, most commercially available antioxidant and soothing cosmetics use single or simply layered active ingredients: 1. They rely solely on purely chemical antioxidants such as vitamin C, vitamin E, and coenzyme Q10, which have drawbacks such as poor stability, strong irritation, and easy oxidation and inactivation; 2. They use single soothing agents such as dipotassium glycyrrhizate and bisabolol, which can suppress inflammation in the short term, but are insufficient in their ability to scavenge ROS and cannot break the vicious cycle of "oxidation-inflammation"; 3. Some formulas, in order to balance moisturizing and repair, blindly increase the content of matrix ingredients such as hyaluronic acid and ceramides, resulting in a heavy and sticky texture, and failing to synergistically amplify the antioxidant-soothing effects.

[0004] In addition, traditional extraction processes often use high temperatures and strong acids / alkalis, which can easily damage the active structure and produce irritating residues, making it difficult to simultaneously meet the multiple requirements of safety, gentleness, and high efficiency. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composite active composition that has the advantages of high antioxidant efficiency, rapid soothing, mild and non-irritating properties, and green and environmentally friendly preparation process.

[0006] To achieve the above objectives, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a composition containing prickly pear fruit extract, wherein, by weight parts, the composition comprises the following:

[0008]

[0009]

[0010] Preferably, the preparation method of the prickly pear fruit extract includes the following steps:

[0011] Step 1-1. Take a certain amount of dried prickly pear fruit, crush it, and sieve it to obtain dried prickly pear fruit powder;

[0012] Steps 1-2. Mix the dried prickly pear fruit powder with petroleum ether and then reflux to extract the powder. Discard the liquid and keep the residue. Dry the residue to obtain defatted fruit powder.

[0013] Steps 1-3. Mix defatted fruit powder with deionized water, heat and extract, centrifuge to collect the supernatant after extraction, concentrate the supernatant under reduced pressure to obtain crude extract;

[0014] Steps 1-4. After mixing the crude extract with anhydrous ethanol, let it stand for 20-24 hours, collect the precipitate, and dry the precipitate to obtain the prickly pear fruit extract.

[0015] More preferably, in step 1-1, the dried prickly pear fruit is crushed and then passed through a 60-mesh sieve.

[0016] More preferably, in steps 1-2, the dried prickly pear fruit powder and petroleum ether are mixed in a reflux extraction device at a mass-to-volume ratio of 1:10-15 g / mL, and refluxed at 60-70℃ for 3 times, each time for 0.8-1 h. The filtrate is discarded and the residue is retained. The residue is dried at 40-50℃ to constant weight to obtain defatted fruit powder.

[0017] More preferably, in steps 1-3, defatted fruit powder and deionized water are mixed at a mass-to-volume ratio of 1:25-30 g / mL, and extracted by water bath heating at 60°C for 12-16 h. After extraction, the supernatant is collected by centrifugation, and the supernatant is concentrated under reduced pressure to 1 / 6 of its original volume to obtain crude extract.

[0018] More preferably, in steps 1-4, after cooling the crude extract to 30-35°C, anhydrous ethanol is added until the final ethanol concentration in the system is 70-80 v / v%. The mixture is then allowed to stand at 30-35°C for 20-24 hours, the precipitate is collected, and the precipitate is dried at 40-50°C for 1-2 hours. Finally, it is freeze-dried at -40 to -30°C for 36-40 hours to obtain the prickly pear fruit extract.

[0019] Preferably, the method for preparing the olive leaf extract includes the following steps:

[0020] Step 2-1. Take dried olive leaves, crush them through a 60-mesh sieve to obtain olive leaf powder;

[0021] Step 2-2. Mix olive leaf powder with 60-70 v / v% ethanol aqueous solution at a mass-volume ratio of 1:20-25 g / mL, and extract with ultrasonic assistance at 25-28 kHz for 35-45 min. After ultrasonic extraction, centrifuge at 5000-6000 r / min for 10 min. Concentrate the supernatant under reduced pressure at a temperature of 50-60℃ until it is reduced to 1 / 10 of its original volume to obtain olive leaf extract.

[0022] In a second aspect, the present invention provides the use of the composition described in the first aspect in the preparation of skin care products having antioxidant, anti-aging, whitening and / or soothing effects.

[0023] Thirdly, the present invention provides an antioxidant and soothing essence lotion, wherein the essence lotion contains the composition described in the first aspect, as well as a moisturizer, an antioxidant, an emulsifier, a preservative, a thickener, a plant oil, a synthetic oil, a pH adjuster, and water.

[0024] Preferably, the composition is added to the serum in an amount of 10-15 wt%.

[0025] Fourthly, the present invention provides a method for preparing the essence lotion described in the third aspect, the method comprising the following steps:

[0026] The essence emulsion is obtained by mixing the aforementioned composition, moisturizer, antioxidant, emulsifier, preservative, thickener, vegetable oil, synthetic oil, pH adjuster and water evenly.

[0027] In this invention:

[0028] The prickly pear fruit extract obtained by ethanol precipitation process is combined with tremella polysaccharide to form a complex polysaccharide system, which enhances the free radical scavenging ability. The introduction of olive leaf extract and centella asiatica extract into the complex polysaccharide system enhances the antioxidant and soothing properties of the system.

[0029] The olive leaf extract obtained by ultrasound-assisted ethanol extraction retains and enriches active ingredients such as oleuropein, hydroxytyrosol, flavonoids, etc., which can synergistically inhibit the release of inflammatory mediators and enhance the soothing effect with other components in the system.

[0030] Centella asiatica extract is rich in active ingredients such as triterpenoid saponins, including asiaticoside, hydroxyasiaticoside, asiatic acid, and hydroxyasiatic acid. It has active effects such as stimulating collagen synthesis, accelerating barrier repair, anti-inflammatory and soothing, relieving sensitivity, and scavenging free radicals. It can synergistically inhibit the release of inflammatory mediators with other components in the system, enhance the soothing effect, synergistically promote antioxidant effects, and enhance the protection against oxidative damage.

[0031] Tremella polysaccharide is a plant-derived acidic heteropolysaccharide. Its high content of hydroxyl and carboxyl groups can form a three-dimensional hydration network in the stratum corneum, effectively increasing skin moisture content and reducing transepidermal water loss. It can also activate the stratum corneum, accelerate stratum corneum renewal, repair barrier damage, reduce the level of inflammatory factors, soothe sensitivity, and reduce inflammatory reactions.

[0032] The beneficial effects of this invention are:

[0033] 1. This invention achieves a synergistic enhancement of antioxidant and soothing effects through a scientific ratio of prickly pear fruit extract, olive leaf extract, centella asiatica extract and tremella polysaccharide, covering multiple skin care needs such as anti-aging and whitening, and overcoming the limitations of single-ingredient efficacy.

[0034] 2. Experiments of this invention show that the absence of any one of the components of prickly pear fruit extract, olive leaf extract, centella asiatica extract, and tremella polysaccharide leads to a significant decrease in the activity of the system, proving that there is a certain synergistic mechanism among the four components, ensuring that the formula has better efficacy in skin care products. Detailed Implementation

[0035] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0036] To further illustrate the present invention, the following embodiments are provided for detailed description. The raw materials used in the following embodiments and comparative examples of the present invention are all commercially available products.

[0037] In this invention:

[0038] Dried prickly pear fruit: The dried fruit of prickly pear (ROSA ROXBURGHI I) is obtained by drying process and is sourced from the plantation of Guanli (Hangzhou) Biotechnology Co., Ltd.

[0039] Olive leaves: Olive (OLEA EUROPAEA) leaves, obtained from Yunzhiqingshui (Tianjin) Technology Co., Ltd.;

[0040] Centella Asiatica Extract: Centella Asiatica extract, purchased from Shanghai Xingye Biotechnology Co., Ltd.;

[0041] Tremella polysaccharide: Tremella (TREMELLA FUCIFORMIS) polysaccharide was purchased from Shanghai Xuntian Biotechnology Co., Ltd.

[0042] Preparation of prickly pear fruit extract:

[0043] Step 1-1. Take a certain amount of dried prickly pear fruit, put it in a high-speed grinder and grind it through a 60-mesh sieve to obtain dried prickly pear fruit powder;

[0044] Step 1-2. Mix the dried prickly pear fruit powder with petroleum ether at a mass-volume ratio of 1:13 g / mL in a reflux extraction device, and reflux extract at 65℃ for 3 times, 1 hour each time. Discard the filtrate and keep the defatted residue. Place the residue in a constant temperature drying oven and dry it at 40-50℃ until constant weight to obtain defatted fruit powder.

[0045] Steps 1-3. Mix defatted fruit powder with deionized water at a mass-to-volume ratio of 1:26 g / mL, and extract by heating in a water bath at 60°C for 14 hours. After extraction, centrifuge to collect the supernatant, and concentrate the supernatant under reduced pressure to 1 / 6 of the original volume to obtain the crude extract.

[0046] Steps 1-4. After cooling the crude extract to 30°C, add anhydrous ethanol until the final ethanol concentration in the system is 80 v / v%. Let it stand at 30°C for 24 h, collect the precipitate, place the precipitate in a constant temperature drying oven and dry it at 40-50°C for 2 h to remove the solvent, and then place it in a vacuum freeze dryer and freeze dry it at -35°C for 36 h to obtain the prickly pear fruit extract.

[0047] Preparation of olive leaf extract:

[0048] Step 2-1. Place dried olive leaves in a high-speed grinder and grind them through a 60-mesh sieve to obtain olive leaf powder;

[0049] Step 2-2. Mix olive leaf powder with 70 v / v% ethanol aqueous solution at a mass-volume ratio of 1:25 g / mL. After mixing, place the mixture in an ultrasonic cleaner and extract with ultrasonic assistance at 28 kHz for 40 min. After ultrasonic extraction, place the mixture in a centrifuge and centrifuge at 5000 r / min for 10 min. Concentrate the supernatant under reduced pressure at a temperature of 55℃ until it is reduced to 1 / 10 of its original volume to obtain olive leaf extract.

[0050] Antioxidant soothing composition

[0051] The raw materials were compounded according to the mass ratio in Table 1 to obtain functional compositions 1-8.

[0052] Table 1. Mass ratio of each component in the composition.

[0053] Raw material name Efficacy Composition 1 Efficacy Composition 2 Efficacy Composition 3 Efficacy Composition 4 prickly pear fruit extract 10 14 17 20 Olive leaf extract 1 2 2 3 Centella Asiatica extract 6 7 8 9 Tremella polysaccharide 0.5 0.6 0.7 0.8 Raw material name Efficacy Composition 5 Efficacy Composition 6 Efficacy Composition 7 Efficacy Composition 8 prickly pear fruit extract 17 17 17 7 Olive leaf extract - 2 2 2 Centella Asiatica extract 8 - 8 8 Tremella polysaccharide 0.7 0.7 - 0.7

[0054] The above-mentioned compounded efficacy compositions 1-8 were mixed with solvents and homogenized at 4000 r / min for 10 min to obtain efficacy solutions 1-8, specifically according to the mass percentages in Table 2.

[0055] Table 2. Mass percentage of each component in the solution

[0056]

[0057] Note: " / " in the table indicates no addition.

[0058] Performance testing

[0059] 1 Antioxidant performance test

[0060] 1.1 DPPH free radical scavenging

[0061] Test solutions: the efficacy solutions 1-8 prepared above;

[0062] The method was modified based on the reference "Optimization of Ultrasonic-Assisted Extraction Process of Cinnamon Polyphenols and Study on its Antioxidant and Antibacterial Activities". 1 mL of the test solution and 1 mL of 0.8 mmol·L⁻¹ were used. -1 Based on DPPH solution, deionized water was added to a total volume of 5 mL as the test group. The solution was placed in the dark for 30 min, and the absorbance at 517 nm was measured.

[0063] A positive control was prepared by replacing the test solution with 100 μg / mL VC, and the rest was the same as the test group.

[0064] Deionized water was used instead of the test solution as a blank group, and the rest was the same as the test group;

[0065] 95% ethanol was used instead of DPPH solution as the background group, and the rest were the same as the test group.

[0066] 1.2 ABTS free radical scavenging

[0067] Test solutions: the efficacy solutions 1-8 prepared above;

[0068] The method was modified based on the reference "Comparative Study on the Content and Antioxidant Activity of Flavonoids in Citrus Peel of Different Cultivated Varieties". 0.5 mL of the test solution and 4 mL of ABTS solution were used as the test group, and the absorbance value at 734 nm was measured.

[0069] A positive control was prepared by replacing the test solution with 100 μg / mL VC, and the rest was the same as the test group.

[0070] Deionized water was used instead of the test solution as a blank group, and the rest was the same as the test group;

[0071] Deionized water was used instead of ABTS solution as the background group, and the rest was the same as the test group.

[0072] 1,3-hydroxyl radical scavenging

[0073] Test solutions: the efficacy solutions 1-8 prepared above;

[0074] The method was modified based on the reference "Effects of Different Fertilization Patterns on Total Polyphenol Content and Antioxidant Activity of Camellia oleifera Cake in Vietnam". 1 mL of the test solution and 1 mL of 9 mmol·L⁻¹ solution were used. -1 FeSO4·7H2O, 1 mL 9 mmol·L -1 Salicylic acid, 1 mL 9 mmol·L -1 Based on H2O2, deionized water was added to a total volume of 10 mL as the test group. After incubation at 37℃ for 10 min, the absorbance at 510 nm was measured.

[0075] A positive control was prepared by replacing the test solution with 100 μg / mL VC, and the rest was the same as the test group.

[0076] Deionized water was used instead of the test solution as a blank group, and the rest was the same as the test group;

[0077] Deionized water was used instead of H2O2 as the background group, and the rest were the same as the test group.

[0078] The formula for calculating the free radical scavenging rate is as follows:

[0079]

[0080] In the formula:

[0081] T 待测 The absorbance is the absorbance of the test solution / VC control.

[0082] T 本底 The absorbance of the sample and other reagents that do not contain DPPH, ABTS, or H2O2;

[0083] T 空白 The absorbance is represented by deionized water instead of the sample.

[0084] The results are shown in Table 3.

[0085] Table 3. Antioxidant Test Results

[0086] Group DPPH free radical scavenging rate / % ABTS free radical scavenging rate / % Hydroxyl radical scavenging rate / % Positive control group 95.3 99.8 12.51 Efficacy Solution 1 94.2 93.1 72.0 Efficacy Solution 2 96.0 94.8 73.1 Efficacy Solution 3 96.8 94.0 72.4 Efficacy Solution 4 95.7 95.0 71.8 Efficacy Solution 5 84.0 82.1 45.5 Efficacy Solution 6 80.2 81.3 52.8 Efficacy Solution 7 88.0 85.2 63.7 Efficacy Solution 8 90.1 91.2 66.8

[0087] Results analysis:

[0088] According to the experimental results, the DPPH scavenging rate and ABTS scavenging rate of efficacy solutions 1-4 are close to or exceed those of the positive control VC, while the hydroxyl radical scavenging rate is significantly better than that of the positive control. This proves that the combination of prickly pear fruit extract, olive leaf extract, centella asiatica extract and tremella polysaccharide has outstanding advantages in combating key free radicals of oxidative damage and can effectively scavenge free radicals to achieve anti-aging effects. Among them, efficacy solution 3 has the best overall performance.

[0089] Efficacy solutions 5-7 are based on the formulation of efficacy solution 3, but with the key components missing, resulting in a decrease in the antioxidant properties of efficacy solutions 5-7. The prickly pear fruit extract provided by this invention contains abundant prickly pear polysaccharides, which have excellent antioxidant effects. This experiment verified that adding olive leaf extract, centella asiatica extract and tremella polysaccharide to the prickly pear polysaccharide system can effectively promote the antioxidant effect of the system, and the combination has outstanding effects.

[0090] The mass ratio of prickly pear fruit extract to tremella polysaccharide in efficacy solution 8 was adjusted, resulting in a less effective antioxidant effect than efficacy solution 3. This indicates that the polysaccharide system formed by prickly pear fruit extract and tremella polysaccharide follows a certain mass ratio.

[0091] 2. Soothing Efficacy Test - Hyaluronidase Inhibition Rate

[0092] 2.1 Experimental Objective: Hyaluronidase is a specific lysing enzyme of hyaluronic acid and a participant in allergic reactions, strongly correlated with histamine release from mast cells. The soothing effect of the test sample can be determined using the hyaluronidase inhibition rate; a higher inhibition rate indicates a stronger soothing effect, and vice versa.

[0093] 2.2 Test solution and control

[0094] Test solution: efficacy solution 1-8; positive control: 1% dipotassium glycyrrhizate aqueous solution; negative control: deionized water.

[0095] 2.3 Experimental Procedure: 500 U / mL hyaluronidase solution; pH 5.4 acetate buffer; 0.5 mg / mL sodium hyaluronate solution;

[0096] Follow the steps below:

[0097]

[0098] Note: The sample solution is either efficacy solution 1-8 or a positive or negative control.

[0099] 2.4 Calculation Formula

[0100]

[0101] In the formula:

[0102] A – Absorbance of reaction system A; B – Absorbance of reaction system B; C – Absorbance of reaction system C; D – Absorbance of reaction system D.

[0103] 2.5 Data Analysis

[0104] The statistical analysis software used was SPSS 19.0. The independent samples t-test was used for comparisons between groups. All statistical analyses were two-tailed tests, and the significance level was α = 0.05. P > 0.05 indicated that there was no significant difference between the two groups, and p < 0.05 indicated that there was a significant difference between the two groups.

[0105] 2.6 Test Results

[0106] Table 4 Results of hyaluronidase inhibition

[0107] Group Inhibition rate / % Positive control group 76.41a negative control group 1.66 Efficacy Solution 1 68.15a Efficacy Solution 2 70.52a Efficacy Solution 3 71.08a Efficacy Solution 4 72.33a Efficacy Solution 5 46.20b Efficacy Solution 6 41.45b Efficacy Solution 7 52.37b Efficacy Solution 8 62.06b

[0108] Note: In the table, "a" indicates a significant difference compared with the negative control group, P < 0.05; "b" indicates a significant difference between the efficacy solution groups 5-8 and the efficacy solution group 3, P < 0.05.

[0109] 2.7 Results Analysis

[0110] As shown in Table 4, the hyaluronidase inhibition rate of solutions 1-4 was significantly higher than that of the negative control group (P < 0.05), and the inhibition rate was close to 76.41% of that of the positive control group. This indicates that the composition provided by the present invention has excellent soothing efficacy and can effectively promote skin barrier repair.

[0111] The hyaluronidase inhibition rate of efficacy solution 3 was significantly higher than that of efficacy solutions 5-8 (P<0.05), indicating that in the complex polysaccharide system formed by prickly pear fruit extract and tremella polysaccharide, olive leaf extract and centella asiatica extract can effectively exert their antioxidant and soothing repair effects. The absence of one of the components will have a certain impact on the performance of the system. Moreover, in this polysaccharide system, prickly pear fruit extract and tremella polysaccharide can achieve better efficacy only when they are in a certain mass ratio.

[0112] Antioxidant and soothing serum

[0113] Weigh the raw materials precisely according to the percentage of mass shown in Table 5;

[0114] Step 3-1. Heat deionized water to 50°C, then add hydroxyethylpiperazine ethane sulfonic acid, bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylic acid ester and 1,3-propanediol in sequence, and stir at 200 r / min to obtain a mixture;

[0115] Step 3-2. Cool the mixture to 35°C, then add the active ingredient 3, glycerin, 1,2-hexanediol, 1,2-butanediol, and p-hydroxyacetophenone in sequence. Stir and mix at 200 r / min to obtain an antioxidant and soothing serum.

[0116] Table 5. Percentage of Raw Materials for the Essence Lotion by Mass

[0117] Raw material name Example 1 Example 2 Example 3 Example 4 Efficacy Composition 3 10% 12% 14% 15% Hydroxyethylpiperazine ethanesulfonic acid 1% 1% 1% 1% bis-diethoxydiethylenecyclohexane 1,4-dicarboxylic acid ester 1% 1% 1% 1% glycerin 1% 1% 1% 1% 1,2-Hexanediol 0.5% 0.5% 0.5% 0.5% 1,3-Propanediol 5% 5% 5% 5% 1,2-Butanediol 0.4% 0.4% 0.4% 0.4% p-Hydroxyacetophenone 0.5% 0.5% 0.5% 0.5% Deionized water Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100%

[0118] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition containing prickly pear fruit extract, characterized in that, The composition comprises, by weight parts, the following components: 10-20 parts of prickly pear fruit extract; Olive leaf extract 1-3 parts; Centella asiatica extract 6-9 parts; 0.5-0.8 parts of Tremella polysaccharide; The preparation method of the prickly pear fruit extract includes the following steps: Step 1-1. Take a certain amount of dried prickly pear fruit, crush it, and sieve it to obtain dried prickly pear fruit powder; Step 1-2. Mix the dried prickly pear fruit powder with petroleum ether at a mass-volume ratio of 1:10-15 g / mL in a reflux extraction device, and reflux extract at 60-70℃ for 3 times, each time for 0.8-1 h. Discard the filtrate and keep the residue. Dry the residue at 40-50℃ to constant weight to obtain defatted fruit powder. Steps 1-3. Mix defatted fruit powder with deionized water at a mass-to-volume ratio of 1:25-30 g / mL, and extract by heating in a water bath at 60℃ for 12-16 h. After extraction, centrifuge to collect the supernatant, and concentrate the supernatant under reduced pressure to 1 / 6 of the original volume to obtain the crude extract. Steps 1-4. After cooling the crude extract to 30-35℃, add anhydrous ethanol until the final ethanol concentration in the system is 70-80 v / v%. Let it stand at 30-35℃ for 20-24 hours, collect the precipitate, dry the precipitate at 40-50℃ for 1-2 hours, and then freeze-dry it at -40 to -30℃ for 36-40 hours to obtain the prickly pear fruit extract. The preparation method of the olive leaf extract includes the following steps: Step 2-1. Take dried olive leaves, crush them through a 60-mesh sieve to obtain olive leaf powder; Step 2-2. Mix olive leaf powder with 60-70 v / v% ethanol aqueous solution at a mass-volume ratio of 1:20-25 g / mL, and extract with ultrasonic assistance at 25-28 kHz for 35-45 min. After ultrasonic extraction, centrifuge at 5000-6000 r / min for 10 min. Concentrate the supernatant under reduced pressure at a temperature of 50-60℃ until it is reduced to 1 / 10 of its original volume to obtain olive leaf extract. The Centella asiatica extract was purchased from Shanghai Xingye Biotechnology Co., Ltd.

2. The composition according to claim 1, characterized in that, In step 1-1, the dried prickly pear fruit is crushed and then passed through a 60-mesh sieve.

3. The use of the composition according to claim 1 or 2 in the preparation of skin care products having antioxidant, anti-aging, whitening and / or soothing effects.

4. An antioxidant and soothing essence lotion, characterized in that, The essence contains the composition according to claim 1 or 2, as well as a moisturizer, antioxidant, emulsifier, preservative, thickener, vegetable oil, synthetic oil, pH adjuster and water; The composition is added to the serum at an amount of 10-15 wt%.

5. The method for preparing the essence lotion according to claim 4, characterized in that, The preparation method includes the following steps: The essence emulsion is obtained by mixing the aforementioned composition, moisturizer, antioxidant, emulsifier, preservative, thickener, vegetable oil, synthetic oil, pH adjuster and water evenly.

Citation Information

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