Preparation method of ginseng essential oil and acne-removing and oil-controlling cell activating enzyme applied to ginseng essential oil

Abstract

The invention relates to the technical field of plant essential oil extraction, in particular to a preparation method of ginseng essential oil and an applied acne-removing and oil-controlling cell activating enzyme. The invention discloses a preparation method of ginseng essential oil, which comprises the following steps of: performing gradient extraction on ginseng powder by using a low-polarity solvent and a high-polarity solvent, centrifuging, distilling and the like to obtain essential oil and a saponin extracting solution, performing water extraction, enzymolysis and the like on residues in sequence to obtain a water-soluble extract, and modifying the water-soluble extract into polysaccharide; then essential oil and saponin are prepared into emulsion, the emulsion and the modified polysaccharide form a pre-polymerization system, and the multi-layer microcapsule containing the ginseng essential oil is obtained through crosslinking of sodium alginate and calcium chloride; finally, compounding with multi-enzyme loaded hydrogel with a four-layer functional structure to prepare the acne-removing and oil-controlling cell activating enzyme with triple effects of resisting bacteria, controlling oil and repairing.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of plant essential oil preparation, and in particular to a ginseng essential oil preparation method and an application of acne-removing and oil-controlling cell active enzyme. BACKGROUND

[0002] The ginseng essential oil preparation method mainly includes supercritical carbon dioxide extraction, microwave-assisted extraction, ultrasonic-assisted extraction and aqueous enzymatic extraction. Among them, the supercritical carbon dioxide extraction is carried out at low temperature, which can maximize the retention of heat-sensitive active ingredients such as ginsenosides and ginseng alkenyl alcohols in ginseng; the microwave extraction combined with emulsion-type solvent can accelerate penetration, shorten extraction time and improve aroma intensity; the ultrasonic-assisted extraction realizes single-cell wall breaking through high-pressure pretreatment and ice-bath temperature control, and improves the extraction rate of functional active ingredients. In addition, ginseng processing waste is used to recover essential oil through solvent extraction and distillation technology, which reduces the cost and realizes resource recycling.

[0003] A patent with the publication number CN119875746A discloses a method for extracting ginseng essential oil by combining eutectic solvent-supercritical technology with column chromatography. First, low-moisture ginseng root is crushed and sieved, and then placed in a supercritical extraction kettle, with anhydrous ethanol and a eutectic solvent composed of a multiple alcohol and a sugar in an equimolar ratio as the entrainer. Then, liquid carbon dioxide is extracted under specific temperature and pressure conditions. Finally, after pressure reduction and concentration, macroporous resin column chromatography is performed, and water, low-concentration ethanol and high-concentration ethanol are used for elution in sequence. The high-concentration ethanol eluate is collected and dried to obtain ginseng essential oil. The patent uses a mixture of anhydrous ethanol and eutectic solvent as an entrainer, and only extracts through a single supercritical stage, without designing a polarity gradient elution, missing the opportunity to enrich high-polarity components. The residue after supercritical extraction is directly discarded, and is not deeply developed.

[0004] A patent with the publication number CN108424817A discloses a method for extracting ginseng essential oil from ginseng processing waste. First, steam before steaming ginseng is collected by a condensing device to prepare steaming water. Then, petroleum ether is added for extraction, and sodium chloride is added for salting out and stirring. After standing and separating, the upper concentrated liquid is separated, and the lower water layer is repeatedly extracted and the concentrated liquid is combined. Subsequently, the concentrated liquid is distilled to prepare crude essential oil. Finally, ginseng essential oil is prepared by vacuum distillation. The patent uses petroleum ether to extract lipophilic components with high efficiency, but the extraction rate of medium-polarity components is low, and full-component enrichment is not achieved. The high-temperature distillation technology used has the risk of accelerating the degradation of heat-sensitive components such as ginseng alkenyl alcohols. The lower water layer is only used for repeated extraction, and the remaining water-soluble components are not utilized, and the residue is not utilized, and the resources are not fully developed. After the essential oil is refined, it is not treated, and the stability is at risk.

[0005] The Chinese patent with publication number CN116785215B discloses a ginseng extract composition nano system composed of ester compounds, vitamin E, plant essential oil, high molecular compounds and other components, which is prepared by mixing and swelling the components in stages according to a specific ratio. The hydrophilic polymer compound in the patent needs to be swollen under specific conditions for a certain period of time, and the temperature and stirring speed need to be strictly controlled in each mixing stage. The requirement for equipment temperature control accuracy and long-term process continuity is high in large-scale production, which may lead to low production efficiency. The nano system needs to rely on passive diffusion for transdermal penetration, and other technologies need to be combined for late application to fully achieve certain effects.

[0006] In the field of acne control and oil control, the application of ginseng essential oil is closely related to cell-vital enzyme technology. The volatile oil components in ginseng essential oil have anti-inflammatory, antibacterial and sebum secretion regulating effects, can inhibit the reproduction of propionibacterium acnes and reduce inflammatory reactions. Cell-vital enzyme can decompose keratin and regulate lipid metabolism, and the combination of the two can synergistically improve pore blockage and inflammation.

[0007] In summary, the preparation of ginseng essential oil needs to seek a balance between efficient extraction, component retention and cost control, while enhancing its efficacy, and further studying its stability and synergistic mechanism to promote industrial application. SUMMARY

[0008] To solve the above problems, the purpose of the present application is to provide a preparation method of ginseng essential oil and an application of acne-removing and oil-controlling cell-vital enzyme, and a preparation method of ginseng essential oil, which specifically comprises the following steps:

[0009] S001, weighing ginseng powder into a beaker, adding a low-polarity solvent, stirring, low-power ultrasonic treatment, adding a high-polarity solvent, medium-power ultrasonic treatment, centrifugal treatment, obtaining precipitate and extract liquid;

[0010] S002, moving the extract liquid to an ice bath tank for cooling, adding a span-80 solution, vortex mixing, and performing extraction and separation treatment, obtaining upper solution and lower solution;

[0011] S003, moving the upper solution to a distillation flask, performing distillation treatment, and collecting the distillate, obtaining ginseng essential oil;

[0012] S004, pumping the lower solution into a thin film evaporator for extraction treatment, obtaining ginsenoside extract liquid;

[0013] S005, moving the precipitate to a beaker, adding ultrapure water for mixing, adding a composite enzyme preparation for treatment, adding activated carbon for adsorption treatment, and adding a complexing agent dropwise, performing two-step water extraction, obtaining ginseng water-soluble extract;

[0014] S006, adding a complex aldehyde-based enzyme preparation and glucose to the ginseng water-soluble extract for aldehyde-based treatment, adding a complex amino enzyme preparation and the permeate for amino treatment, adding a sodium borohydride solution for reduction treatment, adding anhydrous ethanol, centrifugal treatment, collecting the precipitate, washing and vacuum drying, adding ultrapure water, stirring until completely dissolved, adjusting the pH, and the modified ginseng polysaccharide is obtained;

[0015] S007, dropwise adding ginseng essential oil to the ginseng saponin extract solution, and ultrasonic treatment, and the ginseng emulsion is obtained;

[0016] S008, slowly pouring the ginseng emulsion into the modified ginseng polysaccharide, and stirring, and the essential oil-nucleus-saponin layer-polysaccharide shell pre-polymer system is obtained;

[0017] S009, dropwise adding sodium alginate solution and calcium chloride solution to the pre-polymer system, stirring, centrifugal treatment, collecting the microcapsules, washing with ultrapure water 3 times, and freeze-drying treatment, and the multi-layer microcapsule containing ginseng essential oil is obtained.

[0018] The anti-acne and oil-control cell active enzyme is prepared by dispersing different technical carriers in the same main matrix, and specifically includes the following steps:

[0019] S101, weighing chitosan, adding acetic acid solution, stirring, adding lysozyme and lactoferrin, and ultrasonic treatment, and the solution A is obtained;

[0020] S102, weighing sodium tripolyphosphate, adding ultrapure water, stirring until dissolved, slowly dropping into solution A, stirring, centrifugal treatment, collecting the microcapsules, washing and freeze-drying treatment, and the surface rapid release layer material is obtained;

[0021] S103, weighing sodium alginate, adding ultrapure water, stirring until dissolved, adding lipase and protease, and stirring uniformly, and the solution B is obtained;

[0022] S104, dropping solution B into the calcium chloride solution, standing, centrifugal treatment, collecting the microcapsules, washing with ultrapure water 3 times, and freeze-drying treatment, and the middle layer slow release layer material is obtained;

[0023] S105, weighing hyaluronic acid, adding ultrapure water, stirring until dissolved, adding poly-N-isopropyl acrylamide, ammonium persulfate, stirring until dissolved, passing nitrogen, adding superoxide dismutase, quickly pouring into a mold, cross-linking treatment, and cutting, and the deep layer controlled release layer material is obtained;

[0024] S106, weighing hyaluronic acid and chitosan, adding ultrapure water, stirring until dissolved, adjusting the pH, adding hyaluronidase, and stirring until gelatinous, and the main matrix synergistic layer material is obtained;

[0025] S107, in the main matrix coordination layer material, add the multi-layer microcapsule containing ginseng essential oil, the surface layer fast release layer material, the middle layer slow release layer material and the deep layer controlled release layer material, stir, adjust pH, and the multi-layer composite hydrogel is obtained, which is recorded as the acne control oil cell active enzyme.

[0026] The ginseng powder is obtained by washing, drying and crushing fresh ginseng, and then passing through a 40-60 mesh sieve.

[0027] The low-level solvent is composed of n-hexane, cyclohexane and ethanol, and the volume ratio is 160:40:1, and the solid-liquid ratio is 1:2-4.

[0028] The high-level solvent is composed of ethanol and an antioxidant, and the addition amount of the high-level solvent is 30% of the total volume of ethanol, and the concentration of the antioxidant is 0.2%, and the antioxidant is composed of tea polyphenol and vitamin C, and the ratio is 1:1.

[0029] In step S001, the low-power ultrasonic treatment condition is 300 W, 50°C, 10 min, the medium-power ultrasonic treatment condition is 500 W, 55°C, 15 min, and the centrifugation condition is 3000 rpm, 10 min.

[0030] In step S002, the cooling condition is 4°C, 15 min, the concentration of Span-80 is 0.3%, the extraction and separation method is low-speed centrifugation, the upper and lower solutions are collected, the emulsion layer is subjected to high-speed centrifugation, the upper and lower solutions are collected and combined, respectively, wherein the low-speed centrifugation condition is 1500 rpm, 5 min, and the high-speed centrifugation condition is 4000 rpm, 10 min.

[0031] In step S003, the distillation treatment condition is 35°C, -0.09 MPa, and the treatment is stopped until the residual liquid volume in the distillation flask is <10%.

[0032] In step S004, the extraction treatment method is evaporation treatment, the concentrated liquid is collected, vacuum drying is performed, ultrapure water is added, and stirring is performed until complete dissolution, wherein the evaporation treatment condition is 60°C, -0.08 MPa, the vacuum drying condition is 40°C, -0.09 MPa, and the dried material is dissolved in ultrapure water at a mass-volume ratio of 5-10%.

[0033] The mass-volume ratio of the precipitate to ultrapure water in step S005 is 1:2, the method for treating the complex enzyme preparation is adjusting to 3.0-3.5 by 1 mol / L hydrochloric acid, passing nitrogen, treating at 95℃ for 15 min, reducing the temperature to 50℃, adjusting the pH to 5.5, adding the complex enzyme preparation, the enzymolysis conditions are 20 rpm / min, 40 min, the adsorption conditions are 50℃, 10 min, the complexing agent is 0.1% ethylenediaminetetraacetic acid disodium solution, wherein the complex enzyme preparation is composed of cellulase, pectinase, and neutral protease, wherein the cellulase is 1000 U / g of filter residue, the pectinase is 500 U / g of filter residue, and the neutral protease is 300 U / g of filter residue, the two-step water extraction method is reducing the temperature, filtering, collecting the filter residue and the first water extract, adding ultrapure water to the filter residue, heating, centrifuging while hot, collecting the second water extract, and combining the two water extracts, centrifuging, adjusting the pH, and collecting the supernatant, ultrafiltering, collecting the concentrated liquid and the permeate, and adjusting the pH, wherein the temperature reduction conditions are 40℃, 15 rpm / min, and 50 min, the amount of ultrapure water added to the filter residue is the same as the first water extract, the temperature increase conditions are 90℃, 15 rpm / min, and 30 min, the centrifugation conditions are 4000 rpm, 10 min, the centrifugation conditions are 8000 rpm, 15 min, the pH is adjusted to 6-8, the supernatant is concentrated to 100 mL by ultrafiltration through a polyether sulfone membrane with a molecular weight cutoff of 10 kDa, and the pH is adjusted to 5.5-6.0.

[0034] The complex aldehyde-formation enzyme preparation is composed of laccase and glucose oxidase, wherein the laccase is 40 U / mL, and the glucose oxidase is 20 U / mL.

[0035] The complex amino-formation enzyme preparation is 80 U / mL transglutaminase.

[0036] The concentration of glucose in step S006 is 8 mM, the aldehyde-formation treatment conditions are 35℃, 150 rpm / min, and 5 h, the pH is adjusted to 7.5-8.0, the amino-formation treatment conditions are 37℃, 150 rpm / min, and 24 h, the concentration of the amino donor in the permeate is supplemented to 40 mM by L-arginine, the concentration of the sodium borohydride solution is 0.3 mM, the amount of anhydrous ethanol added is 3 times the total volume, the centrifugation treatment conditions are 10000 rpm, 15 min, 2 washes are performed with 70% ethanol, the dry substance is dissolved in ultrapure water at a mass-volume ratio of 4%, and the pH is adjusted to 6.5-7.5.

[0037] The volume ratio of ginseng essential oil to ginsenoside in step S007 is 1:5-9, and the ultrasonic treatment conditions are 200 W, 5 min.

[0038] The volume ratio of the ginseng emulsion and the modified ginseng polysaccharide in step S008 is 1:1-3, and the stirring condition is 500 rpm / min, 5 min.

[0039] The concentration of the sodium alginate solution and the calcium chloride solution in step S009 is 2% and 5 mM, the stirring condition is 500 rpm / min, 30 min, and the centrifugal condition is 8000 rpm, 10 min.

[0040] The mass-volume ratio of the chitosan and the acetic acid solution in step S101 is 1:200, the concentrations of the lysozyme and the lactoferrin are 5000 U / mL and 1 mg / mL respectively, and the ultrasonic treatment condition is 300 W, 10 min.

[0041] The mass-volume ratio of the sodium tripolyphosphate and the ultrapure water in step S102 is 1:167, the stirring condition is 500 rpm / min, 30 min, the centrifugal condition is 8000 rpm, 10 min, and the washing with ultrapure water is performed twice.

[0042] The mass-volume ratio of the sodium alginate and the ultrapure water in step S103 is 1:100, the concentrations of the lipase and the protease are 100 U / mL and 50 U / mL respectively.

[0043] The concentration of the calcium chloride solution in step S104 is 0.2 M, the standing time is 30 min, and the centrifugal condition is 8000 rpm, 10 min.

[0044] The mass-volume ratio of the hyaluronic acid and the ultrapure water in step S105 is 1:50, the concentrations of the lipase and the protease are 100 U / mL and 50 U / mL respectively, the mass ratio of the hyaluronic acid, the poly-N-isopropyl acrylamide and the ammonium persulfate is 1:0.5:0.05, the concentration of the superoxide dismutase is 1000 U / mL, the crosslinking condition is 4 ℃, 2 h, and the cutting is 100 μm micro-pieces.

[0045] The mass-volume ratio of the hyaluronic acid, the chitosan and the ultrapure water in step S106 is 3:2:100, the pH is adjusted to 5.5, the concentration of the hyaluronidase is 50 U / mL, and the stirring condition is 50 rpm / min, 10 min.

[0046] The mass ratio of the multi-layer microcapsule containing ginseng essential oil, the surface layer fast-release layer material, the middle layer slow-release layer material and the deep layer controlled-release layer material in step S107 is 2:3:2:1, the stirring condition is 200 rpm / min, 10 min, and the pH is adjusted to 5.5-6.0.

[0047] Compared with the prior art, the present application has the following beneficial effects:

[0048] 1.The invention adopts mixed solvent gradient extraction method combined with low temperature phase separation technology, through dynamic regulation of solvent polarity by hexane-cyclohexane-ethanol gradient switching and microwave-assisted cell wall breaking, realizes efficient co-extraction of ginseng essential oil and saponin. First, low-polarity solvent is used to extract essential oil, then increase the ethanol to 30% to extract saponin, which shortens the working hours, reduces the solvent consumption, and improves the saponin recovery rate compared with traditional step extraction. Through ice bath combined with Span-80 demulsification, combined with stepwise centrifugation, the thickness of the emulsion layer is reduced. Adding tea polyphenol-vitamin C composite antioxidant, combined with nitrogen protection and reduced pressure low temperature concentration, improves the saponin content, realizes the efficient extraction of essential oil and saponin;

[0049] 2.The invention realizes the deep development of ginseng resources through the integrated technology of whole component extraction-polymer modification-multilayer microcapsule embedding. On the basis of mixed solvent gradient extraction, the core-shell structure microcapsule is constructed by enzyme activation of residues, polysaccharide chemical modification and sodium alginate-calcium chloride crosslinking. First, the double water extraction process is used to improve the polysaccharide yield and amino acid retention rate of the extraction residues, and the utilization rate of raw materials is improved. Through the catalysis of laccase and transglutaminase, the aldehyde group density of polysaccharide is improved, the emulsification activity is increased, and the antioxidant property is improved. The essential oil-saponin emulsion is used as the core, the modified polysaccharide is used as the slow-release layer, and the sodium alginate-calcium chloride is used as the shell, which improves the retention rate of essential oil. The microcapsule realizes biphasic release in the skin environment, reduces the anti-inflammatory activity, and has barrier repair function, which breaks through the waste of residues and the loss of active ingredients in traditional ginseng processing, realizes the efficient conversion from raw materials to functional preparations;

[0050] 3.The application constructs a multi-enzyme load-gradient release-smart hydrogel cross-linking integrated system, which realizes the spatiotemporal precise release of acne-removing and oil-controlling components through the four-layer functional architecture of the surface rapid-release layer, the middle slow-release layer, the deep controlled-release layer and the main matrix coordination layer, and the complex design of microcapsules containing ginseng essential oil. The surface layer is loaded with lysozyme and lactoferrin in chitosan-sodium tripolyphosphate microcapsules, which can quickly destroy the bacterial cell wall after contacting the skin and enhance the antibacterial rate. The middle layer uses calcium alginate microspheres to encapsulate lipase and protease, which can continuously decompose sebum, increase the degradation rate of triacylglycerol and reduce pore blockage. The deep layer uses N-isopropyl acrylamide-hyaluronic acid temperature-sensitive hydrogel to load superoxide dismutase, which is released rapidly under the trigger of body temperature, continuously removes active oxygen in hair follicles and synchronously starts the anti-inflammatory repair mechanism. The main matrix coordination layer adopts a hyaluronic acid-chitosan-hyaluronidase system, which forms pores through enzymatic hydrolysis, improves the transdermal rate of lysozyme and prolongs the skin retention time through viscoelasticity regulation. The release of anti-inflammatory, oil-controlling and repair factors containing ginseng essential oil in the multi-layer microcapsule reduces the expression of inflammatory factors in cooperation with the enzyme system, and ginsenosides increase the transdermal flux of enzymes by regulating tight junction proteins. This technology preserves the activity of superoxide dismutase through low-temperature cross-linking, reduces enzyme activity loss through modular assembly, and finally realizes the spatiotemporal synergy of antibacterial, oil-controlling and repair triple effects, breaking through the technical bottleneck of single action and poor timeliness of traditional acne-removing products.

[0051] 4.The application breaks through the traditional industrial bottlenecks of high cost, low efficiency and low added value in ginseng processing through the whole-chain innovation of extraction-modification-formulation, and constructs a green manufacturing mode from raw materials to terminal products. The whole-dimensional design from molecular structure modification to macroscopic release behavior establishes a whole-process quality control system for ginseng active ingredient extraction-formulation. DETAILED DESCRIPTION

[0052] The application will be further described below in conjunction with specific embodiments.

[0053] Embodiment 1

[0054] A preparation method of ginseng essential oil, specifically comprising the following steps:

[0055] Wash, dry and crush fresh ginseng, and then pass it through a 50-mesh sieve to obtain ginseng powder;

[0056] Weigh 500g of ginseng powder into a beaker, add low-polarity solvent at a material-to-liquid ratio of 1:3, stir, perform low-power ultrasonic treatment, add high-polarity solvent, perform medium-power ultrasonic treatment, and perform centrifugal treatment to obtain precipitate and extraction liquid;

[0057] Transfer the extraction liquid to an ice bath, add a Span-80 solution, vortex mix, and perform extraction and separation treatment to obtain upper and lower solutions;

[0058] The upper layer solution is moved to a distillation flask, and distillation treatment is performed to collect the distillate, thereby obtaining ginseng essential oil.

[0059] The lower layer solution is pumped into a thin film evaporator for extraction treatment, thereby obtaining ginseng saponin extract.

[0060] The ginseng essential oil is added dropwise into the ginseng saponin extract, and ultrasonic treatment is performed, thereby obtaining test sample 1.

[0061] Example 2

[0062] A modified ginseng polysaccharide is prepared by activating residues through enzymatic hydrolysis, extracting polysaccharides through two-water, and modifying polysaccharides through aldehyde and amino groups. The preparation specifically includes the following steps:

[0063] The precipitate in Example 1 is moved to a beaker, and ultrapure water is added for mixing. A composite enzyme preparation is added for treatment, and activated carbon is added for adsorption treatment. A complexing agent is added dropwise, and two-step water extraction is performed, thereby obtaining ginseng water-soluble extract.

[0064] In the ginseng water-soluble extract, a composite aldehyde group enzyme preparation and glucose are added for aldehyde group treatment, and the pH is adjusted. A composite amino group enzyme preparation and a permeate are added for amino group treatment. A sodium borohydride solution is added for reduction treatment. Anhydrous ethanol is added, centrifugal treatment is performed, the precipitate is collected, and washing and vacuum drying are performed. The pH is adjusted after the precipitate is added to ultrapure water in a mass-volume ratio of 4% and stirred until completely dissolved, thereby obtaining test sample 2.

[0065] Example 3

[0066] A multilayer microcapsule containing ginseng essential oil is prepared. The preparation specifically includes the following steps:

[0067] The ginseng essential oil, ginseng saponin extract, and test sample 2 prepared in Example 1 are used.

[0068] The ginseng essential oil is added dropwise to the ginseng saponin extract in a volume ratio of 1:7, and ultrasonic treatment is performed, thereby obtaining a ginseng emulsion.

[0069] The ginseng emulsion is slowly poured into test sample 2 in a volume ratio of 1:2, and stirring is performed, thereby obtaining an essential oil core-saponin layer-polysaccharide shell pre-polymer system.

[0070] Sodium alginate solution and calcium chloride solution are added dropwise to the pre-polymer system, stirring is performed, centrifugal treatment is performed, and the microcapsules are collected. The microcapsules are washed three times with ultrapure water, and freeze-drying treatment is performed, thereby obtaining test sample 3.

[0071] Example 4

[0072] A preparation of acne-removing and oil-controlling cell active enzyme is prepared by dispersing different technical carriers in the same main matrix. The preparation specifically includes the following steps:

[0073] Take 0.5g chitosan, add acetic acid solution, stir, add lysozyme and lactoferrin, ultrasonic treatment, and the solution A is obtained;

[0074] Take 0.3g sodium tripolyphosphate, add ultrapure water, stir until dissolved, slowly drop into solution A, stir, centrifugal treatment, collect microcapsules, wash and freeze-drying treatment, and the surface fast release layer material is obtained;

[0075] Take 1g sodium alginate, add ultrapure water, stir until dissolved, add lipase and protease, and stir evenly to obtain solution B;

[0076] Drop solution B into calcium chloride solution, stand, centrifugal treatment, collect microcapsules, wash with ultrapure water 3 times, and freeze-drying treatment to obtain the middle slow release layer material;

[0077] Take 2g hyaluronic acid, add ultrapure water, stir until dissolved, add poly-N-isopropyl acrylamide, ammonium persulfate, stir until dissolved, pass nitrogen, add superoxide dismutase, quickly pour into the mold, crosslink, and cut to obtain the deep control release layer material;

[0078] Take 3g hyaluronic acid and 2g chitosan, add ultrapure water, stir until dissolved, adjust pH, add hyaluronidase, and stir until gelatinous to obtain the main matrix synergistic layer material;

[0079] In the main matrix synergistic layer material, add test product 3, surface fast release layer material, middle slow release layer material and deep control release layer material, stir, adjust pH, and the test product 4 is obtained.

[0080] Comparative Example 1

[0081] A preparation of ginseng essential oil, using traditional steam distillation method, specifically including the following steps:

[0082] Wash fresh ginseng, dry, crush, and pass through a 50 mesh sieve to obtain ginseng powder;

[0083] Take 500g ginseng powder in a 2000mL round-bottom flask, add ultrapure water at a mass ratio of 1:10, move to a volatile oil extractor connected to a condensation reflux device, set the temperature of the steam generator to 105℃, control the distillation speed to 2-3 drops / second, and continue steam distillation for 4h;

[0084] After the distillation is completed, collect the distillate at the end of the condenser, add anhydrous sodium sulfate, and oscillate the mixture every 10min during the period for 30min to obtain the control product 1.

[0085] Comparative Example 2

[0086] A preparation of ginseng essential oil, prepared by ethanol single solvent, specifically including the following steps:

[0087] Fresh ginseng was washed, dried, crushed, and passed through a 50-mesh sieve to obtain ginseng powder;

[0088] 500 g of the ginseng powder was weighed into a round-bottom flask, 70% ethanol was added at a mass-volume ratio of 1:10, and the mixture was placed in an 80°C water bath for heating and reflux extraction for 2 h. The hot mixture was filtered under reduced pressure, and the filtrate was collected.

[0089] The residue was again extracted with 70% ethanol at the same temperature, and the hot mixture was filtered under reduced pressure. The filtrate was collected, combined with the filtrate obtained in the first extraction, and transferred to a rotary evaporator. The mixture was concentrated under reduced pressure at 45°C until no alcohol odor was detected, to obtain Reference 2.

[0090] Comparative Example 3

[0091] An unmodified ginseng polysaccharide was extracted by hot water extraction, which included the following steps:

[0092] The precipitate in Example 1 was transferred to a round-bottom flask, ultrapure water was added at a mass-volume ratio of 1:10, and the mixture was placed in a 90°C constant-temperature water bath for extraction for 2 h. The hot mixture was filtered, and the filtrate was collected.

[0093] The residue was again extracted with ultrapure water at the same temperature, and the hot mixture was filtered under reduced pressure. The filtrate was collected, combined with the filtrate obtained in the first extraction, and transferred to a rotary evaporator. The mixture was concentrated under reduced pressure at 60°C and -0.08 MPa, to obtain Reference 3.

[0094] Comparative Example 4

[0095] A physically mixed modified ginseng polysaccharide was prepared by physical mixing, which included the following steps:

[0096] Reference 3 was mixed with glucose and L-arginine at a mass ratio of 1:2:1 to obtain Reference 4.

[0097] Comparative Example 5

[0098] A single-layer microcapsule with no core-shell structure was prepared, which included the following steps:

[0099] The method of Example 4 was used, except that the test sample 3 was not added with modified ginseng polysaccharide during the preparation, to obtain Reference 5.

[0100] Comparative Example 6

[0101] A pimple-removing gel containing 2% salicylic acid was used to obtain Reference 6.

[0102] Experimental Example 1

[0103] The extraction efficiency of the test sample of Example 1 and the control samples of Comparative Examples 1-2 is verified in the present experimental example, by determining the essential oil yield, total saponin content, and saponin Rg1 content, specifically including the following steps:

[0104] 1. Essential oil yield determination:

[0105] The test sample 1, the control sample 1, and the control sample 2 are respectively placed in an already constant weight evaporating dish, each sample is 3 parallel, dried to constant weight at 40℃, -0.09MPa, and the essential oil mass after constant weight is recorded;

[0106] The essential oil yield is calculated according to the ginseng powder, and the determination results are shown in Table 1.

[0107] 2. Total saponin content determination:

[0108] 1g vanillin powder is weighed into a beaker, dissolved with a small amount of anhydrous ethanol, transferred to a 100mL volumetric flask, and washed and diluted with anhydrous ethanol, thereby obtaining a 1% vanillin ethanol solution;

[0109] 70mL of perchloric acid is measured and slowly injected into 30mL of ultrapure water while stirring, thereby obtaining a 70% perchloric acid solution;

[0110] 10mg of ginsenoside Re is weighed into a beaker, dissolved with a small amount of methanol, transferred to a 10mL volumetric flask, and washed and diluted with methanol, thereby obtaining a ginsenoside Re standard solution;

[0111] 50mg of the test sample 1, the control sample 1, and the control sample 2 are respectively weighed into a beaker, dissolved with a small amount of methanol, transferred to a 25mL volumetric flask, and washed and diluted with methanol, thereby obtaining the sample to be tested;

[0112] 20μL, 40μL, 80μL, 120μL, 160μL, and 200μL of the ginsenoside Re standard solution and 50μL of the sample to be tested are respectively transferred to a centrifugal tube with a stopper, evaporated at 40℃, 0.5mL of 1% vanillin perchloric acid solution is added, and after being fully mixed at 60℃ constant temperature water bath for 15min, immediately cooled with ice water for 2min, 5mL of 77% sulfuric acid solution is added accurately, and the absorbance is measured at 540nm wavelength after eliminating bubbles with reagent blank.

[0113] The standard curve is drawn with the standard solution concentration as the abscissa and the corresponding absorbance value as the ordinate, the total saponin content is calculated according to the dried product, and the determination results are shown in Table 1.

[0114] 3. Saponin Rg1 content determination:

[0115] Take 30 mg of ginsenoside Rg1 into a beaker, dissolve with a small amount of methanol, transfer to a 100 mL volumetric flask, and wash and dilute with methanol, to obtain a ginsenoside Rg1 standard solution;

[0116] Respectively, weigh 30 mg of test sample 1, control sample 1 and control sample 2 into a beaker, dissolve with a small amount of methanol, transfer to a 10 mL volumetric flask, and wash and dilute with methanol, filter, and take the filtrate, to obtain the sample to be tested;

[0117] Instrument conditions: the chromatographic column is a C18 column, 250 mm x 4.6 mm, 5 μm; the mobile phase A is acetonitrile, and the mobile phase B is a 0.1% phosphoric acid solution; the flow rate is 1.3 mL / min; the gradient is 0-30 min, 81% mobile phase B, 30-35 min, 81-76% mobile phase B, 35-60 min, 76-60% mobile phase B; the column temperature is 30°C; the detection wavelength is 203 nm, and the injection amount is 20 μL

[0118] Take the standard solution concentration as the abscissa, and the corresponding absorbance value as the ordinate, to draw a standard curve, calculate the ginsenoside Rg1 content according to the dried product, and the determination results are shown in Table 1.

[0119] Table 1 Extraction efficiency

[0120] Sample Essential oil yield (%) Total saponin content (%) Saponin Rg1 content (%) Example 1 1.9±0.4 29.5±1.2 95.8±1.5 Comparative Example 1 1.1±0.3 14.8±1.1 70.8±1.4 Comparative Example 2 0.6±0.4 20.4±1.0 81.2±1.5

[0121] As can be seen from Table 1, the essential oil yield of Example 1 is 1.9 ± 0.4%, which is significantly higher than that of Comparative Examples 1 and 2, indicating that the gradient switching solvent polarity combined with microwave-assisted wall breaking can more efficiently extract ginseng essential oil, and the traditional distillation and single ethanol extraction have poor effects. The total saponin content of Example 1 reaches 29.5 ± 1.2%, which is much higher than that of Comparative Examples 1 and 2, showing that the co-extraction method has obvious advantages in enriching total saponins, and traditional and single solvent extraction cannot reach a similar level. The saponin Rg1 content of Example 1 is 95.8 ± 1.5%, which is higher than that of Comparative Examples 1 and 2, indicating that the co-extraction technology has better extraction efficiency for specific high-value saponin Rg1, and can improve the quality of the extract.

[0122] In summary, the dynamic regulation of the solvent polarity of the gradient switching of hexane-cyclohexane-ethanol and the co-extraction method of microwave-assisted wall breaking can significantly improve the yield and content compared with traditional water vapor distillation and ethanol single solvent extraction in the extraction of ginseng essential oil and saponins, and has better extraction effect, which helps to improve the quality and utilization value of ginseng extract.

[0123] Experimental Example 2

[0124] The experiment example modifies the polysaccharide function characterization in the test sample of example 2 and the control sample of comparative examples 3-4, by determining the emulsification activity index, emulsification stability index, DPPH clearance rate, which specifically includes the following steps:

[0125] 1. Polysaccharide yield determination:

[0126] Respectively, the test sample 2, the control sample 3 and the control sample 4 are placed in the already constant weight evaporation dish, each sample 3 parallel, dried to constant weight at 40℃, -0.09MPa, freeze-dried, and the polysaccharide mass after constant weight is recorded;

[0127] The polysaccharide yield is calculated according to the ginseng powder, and the determination results are shown in Table 2.

[0128] 2. Emulsification activity index and emulsification stability index determination:

[0129] Respectively, 8mg of test sample 2, control sample 3 and control sample 4 are weighed, 8mL of ultrapure water and 2mL of soybean oil are added to the centrifuge tube, and the emulsion is obtained by homogenizing at 20000r / min for 3min by high-speed shearing machine;

[0130] At 0 and 10min, 50μL of bottom layer emulsion is taken and added to 5mL of 0.1% sodium dodecyl sulfate solution, vortexed for 30 seconds, and the absorbance is measured at 500nm wavelength, with reagent as blank;

[0131] According to the absorbance at 0 and 10min, the emulsification activity index and emulsification stability index are calculated, and the determination results are shown in Table 2.

[0132] 3. DPPH clearance rate determination:

[0133] Respectively, 5mg of test sample 2, control sample 3 and control sample 4 are weighed, 1mL of ultrapure water is added, and then transferred to a test tube with a plug, 3mL of 0.1mM DPPH ethanol solution is slowly added, mixed uniformly, reacted for 30min in the dark at room temperature, and the absorbance is measured at 517nm wavelength, with reagent as blank, each sample 3 parallel;

[0134] According to the ratio of sample absorbance to blank absorbance, the DPPH clearance rate is calculated, and the determination results are shown in Table 2.

[0135] Table 2 Polysaccharide modification function characterization

[0136]

[0137] As can be seen from Table 2, the polysaccharide yield of Example 2 is as high as 95.7±1.2%, which is much higher than that of Comparative Example 3 and Comparative Example 4. It is shown that the process of enzymatic hydrolysis, aqueous two-phase extraction combined with chemical modification can greatly improve the extraction rate of ginseng polysaccharide, and the traditional hot water extraction and simple physical mixing modification have poor effects. The emulsification activity index of Example 2 is 79.4±1.1m 2 / g, which is significantly higher than that of Comparative Example 3 and Comparative Example 4, indicating that the emulsification activity of the polysaccharide after chemical modification is greatly improved, and the physical mixing modification does not effectively improve the performance. The emulsion stability index of Example 2 is 187.4±1.1min, which is much better than that of Comparative Example 3 and Comparative Example 4, which shows that chemical modification can enhance the emulsion stability of polysaccharide, and traditional extraction and physical mixing are difficult to achieve. The DPPH clearance rate of Example 2 is 93.8±1.0%, which is much higher than that of Comparative Example 3 and Comparative Example 4, indicating that enzymatic hydrolysis, aqueous two-phase extraction combined with aldehyde group-amination modification can significantly improve the antioxidant capacity of ginseng polysaccharide, and the traditional extraction and physical mixing modification have weak effects on the antioxidant capacity of polysaccharide.

[0138] In summary, the enzymatic activation residue-aqueous two-phase extraction-chemical modification process of Example 2 is superior to the traditional hot water extraction of Comparative Example 3 and the physical mixing modification of Comparative Example 4 in terms of ginseng polysaccharide yield, emulsification characteristics and antioxidant capacity, and can prepare modified ginseng polysaccharide with better performance, providing better raw materials for the application of polysaccharide in food, medicine and other fields.

[0139] Experimental Example 3

[0140] In this experimental example, the microcapsule encapsulation rate of the oil-saponin-modified polysaccharide core-shell microcapsule of the test sample of Example 3 was determined by measuring the contents of encapsulated oil and saponin, which included the following steps:

[0141] The test sample 3 was placed in a 50 mL conical flask with a stopper, 10 mL of methanol was added, and ultrasonic treatment was performed at 300 W for 10 min. Then, the sample was transferred to a centrifuge tube and centrifuged at 12000 rpm for 15 min. The supernatant was removed, and the filtrate was obtained by filtration through a 0.22 μm filter membrane. The embedded material was obtained, wherein the ginseng emulsion in Example 3 was the total input amount, and each sample was tested in triplicate.

[0142] The essential oil components in the embedding material and the ginseng emulsion in Example 3 were determined by gas phase tandem mass spectrometry, and the instrument conditions were as follows: the chromatographic column was an HP-1 column, 250 μm x 30 m, 0.25 μm; the injection port temperature was 280 ℃; the temperature program was as follows: the initial temperature was 60 ℃, and was kept for 3 min; the temperature was increased to 180 ℃ at a rate of 10 ℃ / min from 60 ℃, and was kept for 3 min; the temperature was increased to 220 ℃ at a rate of 3 ℃ / min, and was kept for 10 min; the temperature was increased to 280 ℃ at a rate of 10 ℃ / min, and was kept for 10 min; the carrier gas was helium; the column internal carrier gas flow was 1.00 mL / min; the split ratio was 50:1; the injection amount was 1 μL; the vaporization chamber temperature was 280 ℃; the EI ion source was used, the electron energy was 70 eV; the ion source temperature was 230 ℃; the interface temperature was 280 ℃; the solvent delay was 2 min; the multiplier voltage was 2141.2 V; and the scanning range was 20-750 min. The content of ginsenoside Rg1 in the embedding material and the ginseng emulsion in Example 3 was determined by the determination method of ginsenoside Rg1 content in Experimental Example 1, the ratio of the embedding material to the ginseng emulsion was determined, and the encapsulation rates of the essential oil and the ginsenoside were calculated.

[0143] The encapsulation rate of the essential oil was 98.5 ± 0.9%, and the encapsulation rate of the ginsenoside was 91.1 ± 1.1%, both of which were at a high level, indicating that the integrated technology could effectively embed the ginseng essential oil and the ginsenoside, reduce the loss of active ingredients, and be beneficial to the preservation and subsequent application of active ingredients in the deep development of ginseng resources.

[0144] Experimental Example 4

[0145] In this experimental example, the in-vitro release of the test sample in Experimental Example 4 was determined, and the specific steps included the following:

[0146] 10 mg of the test sample 4 was weighed into a dialysis bag and immersed in 50 mL of 1X PBS with a pH of 5.5, and 3 parallel samples were prepared.

[0147] 1. Lysozyme concentration

[0148] 10 mg of the lysozyme standard was weighed into a beaker, dissolved with a small amount of ultrapure water, transferred to a 10 mL volumetric flask, washed with ultrapure water, and made up to volume, and shaken to obtain a lysozyme standard stock solution;

[0149] 0, 0.5, 0.1, 0.05 mL of the lysozyme standard stock solution was respectively taken into a test tube, supplemented with ultrapure water to 1 mL, and shaken to obtain a standard working solution;

[0150] 0.2 mL of the leaching liquid was respectively taken at 5 min, 10 min, 30 min, 1 h, 2 h and 12 h, and an equal amount of 1X PBS with a pH of 5.5 was added;

[0151] The 0.2 mL leaching liquid was supplemented with ultrapure water to 1 mL, and shaken to obtain a sample to be tested;

[0152] The concentration of lysozyme was detected by high performance liquid chromatography. The instrument conditions were as follows: a C18 column; a mobile phase of 0.1% phosphoric acid aqueous solution-acetonitrile at a volume ratio of 85:15; a flow rate of 1.0 mL / min; a detection wavelength of 214 nm; and a sample injection amount of 20 μL;

[0153] The standard curve was plotted with the lysozyme concentration of the standard working solution as the abscissa and the peak area as the ordinate. The cumulative release rate was calculated according to the total input amount, and the determination results are shown in Table 4-1.

[0154] Table 4-1 Release of lysozyme in the surface layer

[0155] Time Cumulative release rate of lysozyme (%) 5 min 86.4±1.0 10 min 88.1±1.1 30 min 89.7±1.2 1h 91.4±1.1 2h 93.5±1.0 12h 95.7±1.2

[0156] 2. Lipase activity

[0157] 13.9 mg of p-nitrophenol was weighed and dissolved in 100 mL of ultrapure water to obtain a p-nitrophenol standard stock solution. 0, 0.1, 0.2, 0.5, 1.0, and 2.0 mL of the p-nitrophenol standard stock solution were respectively transferred to test tubes, 50 mM pH 8.0 Tris-HCl buffer was added to make up to 1 mL, 2 mL of isopropyl alcohol was added, and vortex mixing was performed to obtain a standard working solution.

[0158] 50 μL of the leaching liquid was taken at 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h, and an equal amount of 1X PBS at pH 5.5 was added.

[0159] 100 μL of 2 mM calcium chloride and 100 μL of 0.5 mM p-nitrophenol palmitate were added to 50 μL of the leaching liquid, 50 mM pH 8.0 Tris-HCl buffer was added to make up to 1 mL, and the mixture was reacted at 37°C for 10 min. Then, 2 mL of isopropyl alcohol was immediately added, vortex mixing was performed, centrifugation was performed at 10,000 r / min for 15 min, and the supernatant was taken in a cuvette to obtain a sample to be tested.

[0160] The reagent was used as a blank control, and the absorbance of the standard working solution, the sample to be tested, and the blank control was measured at 410 nm.

[0161] The standard curve was plotted with the concentration of the standard working solution as the abscissa and the corresponding absorbance value as the ordinate, the concentration of p-nitrophenol in the sample to be tested was calculated, the lipase activity was calculated according to the reaction time and the volume of the leaching liquid, and the cumulative release rate was calculated according to the total input amount. The determination results are shown in Table 4-2.

[0162] Table 4-2 Lipase release in the middle layer

[0163] Time Cumulative release rate of lipase (%) 1h 30.2±1.2 2h 38.0±1.1 4h 47.3±1.0 6h 58.2±1.3 12h 71.5±1.3 24h 86.7±1.3

[0164] 3. Superoxide dismutase activity

[0165] In 2.8 mL of 50 mM Tris-HCl buffer at 25°C, 0.2 mL of 4 mM pyrogallol was added, vortexed to mix, and then the autoxidation control group was obtained;

[0166] 0.2 mL of the leaching solution was taken at 6 h, 12 h, 24 h, and 48 h, and an equal amount of 1X PBS at pH 5.5 was added;

[0167] In 0.2 mL of the leaching solution, 2.6 mL of 50 mM Tris-HCl buffer at 25°C was added, 0.2 mL of 4 mM pyrogallol was added, and vortexed to mix, and then the test sample was obtained;

[0168] At a wavelength of 420 nm, the absorbance was measured every 30 seconds for 4 min, the average increase in absorbance per minute was calculated, the inhibition rate was calculated according to the ratio of the average increase in absorbance per minute of the test sample to the control, and the amount of enzyme required to inhibit the autoxidation rate of pyrogallol by 50% was defined as 1 unit of activity. The amount of enzyme was taken as the abscissa, and the inhibition rate was taken as the ordinate, a curve was drawn or linear regression was performed, the amount of enzyme corresponding to an inhibition rate of 50% was found, the superoxide dismutase activity was calculated, and the cumulative release rate was calculated according to the total input amount. The test results are shown in Table 4-3.

[0169] Table 4-3 Deep superoxide dismutase controlled release

[0170] Time Cumulative release rate of superoxide dismutase (%) 6h 27.0±1.3 12h 37.9±1.0 24h 51.7±1.2 48h 70.5±1.3

[0171] As can be seen from Table 4-1, the lysozyme was released rapidly at 5-30 min, approached saturation at 1 h, reached 93.5±1.0% at 2 h, and still maintained 95.7±1.2% at 12 h. This shows that the surface fast-release layer allows the lysozyme to act immediately, releases at a high concentration in a short time, quickly responds to the initial demand for acne removal and oil control, and has subsequent sustained release to ensure the efficacy of the early stage. The lipase started to release at 1 h, increased with time, reached 71.5±1.3% at 12 h, and 86.7±1.3% at 24 h. This reflects the slow and sustained release characteristics of the middle slow-release layer, which connects with the fast release of lysozyme to form a medium-term component supply to maintain the effect of acne removal and oil control. The superoxide dismutase started to release at 6 h, reached 37.9±1.0% at 12 h, 51.7±1.2% at 24 h, and 70.5±1.3% at 48 h. This shows the long-acting and slow-release characteristics of the deep controlled-release layer, which continuously outputs in the later stage and cooperates with the previous two layers to achieve a gradient release of components.

[0172] In combination with Table 4-2, 4-3, it can be seen that the release rules of the surface lysozyme immediate-release layer, the middle layer lipase sustained-release layer, and the deep layer superoxide dismutase controlled-release layer are completely matched with the four-layer functional architecture design. Through the continuity of the release of each enzyme, lysozyme is still highly released for 12 h, and lipase is continuously released for 24 h, it is speculated that the main matrix plays a role in stabilizing the system and assisting gradient release, verifying the scientificity of the architecture design.

[0173] Under the multi-enzyme loading, different enzymes are released according to the requirements of acne removal and oil control in the time dimension of 5 min to 48 h, that is, lysozyme attacks first, lipase stabilizes continuously, and superoxide dismutase synergistically works for a long time, which matches the spatiotemporal precise target. In combination with ginseng essential oil microcapsule compounding, it is speculated that it can further synergistically regulate the skin microenvironment and improve the acne removal and oil control effect, showing the efficiency and precision of the integrated system in the delivery of functional ingredients, and providing technical support for the development of precise skin care products.

[0174] In summary, the data of the present application clearly shows that the integrated system releases the acne removal and oil control ingredients in the time sequence of immediate-release, sustained-release, and controlled-release through the four-layer architecture, realizes spatiotemporal precise delivery, verifies the effectiveness and advantages of the technical design, and provides a feasible scheme for the precise skin care scene.

[0175] Experimental Example 5

[0176] In this experimental example, the acne removal and oil control efficacy of the test product of Example 4 and the control products of Comparative Examples 5 and 6 was evaluated, which specifically included the following steps:

[0177] 1. Experimental method:

[0178] Select 90 Pillsbury grade II-III acne patients aged 18-35 years old, and who have not used antibiotics or hormone drugs in the past month, and randomly divide them into 3 groups;

[0179] After cleaning the face, apply the test product 4, the control product 5, and the control product 6 topically in the morning and evening every day, with a dosage of 2 mg / cm 2 The acne area is measured before use and after 4 weeks.

[0180] 2. Measurement:

[0181] The number of inflammatory papules and pustules is counted using a skin detector, the acne reduction rate is calculated according to the ratio before and after use, and the measurement results are shown in Table 5.

[0182] The oil content on both sides of the nose wing is measured using a skin oil tester, the oil secretion reduction rate is calculated according to the ratio before and after use, and the measurement results are shown in Table 5.

[0183] The water content of the forehead stratum corneum is measured using a skin moisture tester, the skin moisture increase rate is calculated according to the ratio before and after use, and the measurement results are shown in Table 5.

[0184] The skin swab was taken, and the IL-6 concentration was detected by an ELISA kit. According to the ratio before and after use, the reduction rate of inflammatory factors was calculated, and the test results are shown in Table 5.

[0185] Table 5: Effects of acne removal and oil control of volunteers after use

[0186]

[0187] As can be seen from Table 5, the acne reduction rate of Example 4 is 82.4±1.0%, which is significantly higher than that of Comparative Example 5 and Comparative Example 6. It shows that the multi-enzyme loading-gradient release-smart hydrogel cross-linking integrated system of Example 4 has a better effect on reducing the number of acne. The single-core-shell structure and single salicylic acid gel cannot achieve similar acne-removing effects. The oil secretion reduction rate of Example 4 is 76.7±0.9%, which is much higher than that of Comparative Example 5 and Comparative Example 6. It reflects that the integrated system combined with ginseng essential oil microcapsule compounding and four-layer functional architecture can more efficiently regulate oil secretion. The single-core-shell structure and salicylic acid gel have a weak effect on improving oil secretion. The skin moisture increase rate of Example 4 is 45.8±1.0%, which is significantly higher than that of Comparative Example 5 and Comparative Example 6. It shows that the integrated system can effectively improve the skin moisture and skin barrier while removing acne and controlling oil. The single-core-shell structure and salicylic acid gel have limited positive effects on skin moisturizing. Even salicylic acid may affect the moisture state due to its irritant properties. The inflammatory factor reduction rate of Example 4 is 64.1±0.9%, which is higher than that of Comparative Example 5 and Comparative Example 6. It shows that the integrated system can more efficiently reduce skin inflammation. The single-core-shell structure lacks modified ginseng polysaccharide design and has insufficient inflammation regulation. Although salicylic acid has certain anti-inflammatory properties, its effect is not as good as that of the integrated system.

[0188] In summary, the multi-enzyme loading-gradient release-smart hydrogel cross-linking integrated system of Example 4, through four-layer functional architecture and microcapsule compounding design, is superior to the single-layer microcapsule of Comparative Example 5 and the acne-removing gel containing 2% salicylic acid of Comparative Example 6 in terms of acne removal, oil control, moisturizing, and anti-inflammatory. It exhibits better comprehensive efficacy in acne-removing, oil-controlling, and skin-care products, and provides a more advantageous technical solution for precisely and efficiently improving the state of acne skin.

[0189] The above description is only used to illustrate the technical solutions of the present application and not to limit them. Any equivalent modification and change of the technical solutions of the present application made by those skilled in the art without departing from the overall concept of the present application still belongs to the scope of the present application.

Claims

1. A method of preparing a ginseng essential oil, characterized by: The preparation method of the ginseng essential oil specifically comprises the following steps: S001, weighing ginseng powder into a beaker, adding a low-polarity solvent, stirring, low-power ultrasonic treatment, adding a high-polarity solvent, medium-power ultrasonic treatment, centrifugal treatment, and obtaining precipitate and extract liquid; S002, moving the extract liquid to an ice bath tank for cooling, adding a Span-80 solution, vortex mixing, performing extraction separation treatment, and obtaining upper solution and lower solution; S003, moving the upper solution to a distillation flask for distillation treatment, collecting the distillate, and obtaining ginseng essential oil; S004, pumping the lower solution into a thin film evaporator for extraction treatment, and obtaining ginseng saponin extract liquid; S005, moving the precipitate to a beaker, adding ultrapure water for mixing, adding a composite enzyme preparation for treatment, adding activated carbon for adsorption treatment, and adding a complexing agent dropwise for two-step water extraction, and obtaining ginseng water-soluble extract; S006, adding a composite aldehyde group enzyme preparation and glucose to the ginseng water-soluble extract for aldehyde group treatment, adding a composite amination enzyme preparation and a permeate for amination treatment, adding a sodium borohydride solution for reduction treatment, adding anhydrous ethanol, centrifugal treatment, collecting the precipitate, washing and vacuum drying, adding ultrapure water, stirring until completely dissolved, and adjusting the pH, and obtaining modified ginseng polysaccharide; S007, adding the ginseng essential oil dropwise to the ginseng saponin extract liquid, and performing ultrasonic treatment, and obtaining ginseng emulsion; S008, slowly pouring the ginseng emulsion into the modified ginseng polysaccharide, and stirring, and obtaining an essential oil core-saponin layer-polysaccharide shell pre-polymer system; S009, adding sodium alginate solution and calcium chloride solution to the pre-polymer system, stirring, centrifugal treatment, collecting microcapsules, washing with ultrapure water for 3 times, and freeze-drying treatment, and obtaining multilayer microcapsules containing ginseng essential oil.

2. The method of claim 1, wherein the ginseng essential oil is prepared by the steps of: The acne-removing and oil-controlling cell active enzyme is prepared by dispersing different technical carriers in the same main matrix, and specifically comprises the following steps: ​ S101, weighing chitosan, adding acetic acid solution, stirring, adding lysozyme and lactoferrin, and performing ultrasonic treatment, and obtaining solution A; S102, weighing sodium tripolyphosphate, adding ultrapure water, stirring until dissolved, slowly dropping into solution A, stirring, centrifugal treatment, collecting microcapsules, and washing and freeze-drying treatment, and obtaining a surface rapid release layer; S103, weighing sodium alginate, adding ultrapure water, stirring until dissolved, adding lipase and protease, and stirring uniformly, and obtaining solution B; S104, dropping solution B into a calcium chloride solution, standing, centrifugal treatment, collecting microcapsules, washing with ultrapure water for 3 times, and freeze-drying treatment, and obtaining a middle layer sustained release layer; S105, weighing hyaluronic acid, adding ultrapure water, stirring until dissolved, adding poly-N-isopropyl acrylamide and ammonium persulfate, stirring until dissolved, passing nitrogen, adding superoxide dismutase, quickly pouring into a mold, crosslinking treatment, and cutting, and obtaining a deep layer controlled release layer; S106, weighing hyaluronic acid and chitosan, adding ultrapure water, stirring until dissolved, adjusting the pH, adding hyaluronidase, and stirring until gelatinous, and obtaining a main matrix synergistic layer. S107, in the main matrix, add the multi-layer microcapsule containing ginseng essential oil, the surface layer of the rapid release layer, the middle layer of the slow release layer and the deep layer of the controlled release layer, stir, adjust the pH, and the multi-layer composite hydrogel is obtained, which is recorded as the anti-acne and oil-control cell active enzyme.

3. The method for preparing ginseng essential oil according to claim 1, characterized in that: The ginseng powder is prepared by washing, drying and crushing fresh ginseng, and then sieving through a 40-60 mesh screen.

4. The method for preparing ginseng essential oil according to claim 1, characterized in that: The low polarity solvent is composed of n-hexane, cyclohexane and ethanol, with a volume ratio of 160:40:1, and a material-liquid ratio of 1:2-4. The high polarity solvent is composed of ethanol and an antioxidant, with the ethanol accounting for 30% of the total volume, and the antioxidant having a concentration of 0.2%. The antioxidant is composed of tea polyphenol and vitamin C, with a ratio of 1:

1.

5. The method of claim 1, wherein the ginseng essential oil is prepared by the steps of: The concentration of Span-80 is 0.3%. The extraction and separation method is low-speed centrifugation to collect the upper and lower solutions, and high-speed centrifugation to collect the upper and lower solutions of the emulsion layer and then combine them. The low-speed centrifugation is performed at 1500 rpm for 5 min, and the high-speed centrifugation is performed at 4000 rpm for 10 min. ​ 6. The method of claim 1, wherein the ginseng essential oil is prepared by the steps of: In step S004, the dried substance is dissolved in ultrapure water at a mass-volume ratio of 5-10%. The extraction method is evaporation treatment. The concentrated solution is collected, dried under reduced pressure, and then ultrapure water is added and stirred until completely dissolved. In step S005, the precipitate and ultrapure water have a mass-volume ratio of 1:

2. The complexing agent is 0.1% ethylenediaminetetraacetic acid disodium solution. The two-step water extraction method involves cooling, filtering, collecting the filter residue and the first water extract, adding ultrapure water to the filter residue, heating, and then collecting the second water extract. The two water extracts are combined, centrifuged, the pH is adjusted to 6-8, the supernatant is collected, ultrafiltration is performed, and the concentrated solution and the permeate are collected. The pH is adjusted to 5.5-6.

0. In step S006, the concentration of glucose is 8 mM. The concentration of the amino donor in the permeate is supplemented to 40 mM by adding L-arginine. The concentration of sodium borohydride solution is 0.3 mM. The amount of anhydrous ethanol added is 3 times the total volume. The dried substance is dissolved in ultrapure water at a mass-volume ratio of 4%. ​ 7. The method of claim 1, wherein the ginseng essential oil is prepared by the steps of: The complex enzyme preparation is composed of cellulase, pectinase and neutral protease. The cellulase is 1000 U / g of filter residue, the pectinase is 500 U / g of filter residue, and the neutral protease is 300 U / g of filter residue. The complex aldehyde-based enzyme preparation is composed of laccase and glucose oxidase. The laccase is 40 U / mL, and the glucose oxidase is 20 U / mL. The complex amino-based enzyme preparation is 80 U / mL of transglutaminase. ​ 8. The method of claim 1, wherein the ginseng essential oil is prepared by the steps of: The volume ratio of ginseng essential oil to ginsenoside is 1:5-9, and the volume ratio of ginseng emulsion to modified ginseng polysaccharide is 1:1-3. ​ 9. The method of claim 2, wherein the ginseng essential oil is prepared by the steps of: The mass-volume ratio of chitosan to acetic acid solution in step S101 is 1:200, the concentrations of lysozyme and lactoferrin are 5000 U / mL and 1 mg / mL respectively, the mass-volume ratio of sodium tripolyphosphate to ultrapure water in step S102 is 1:167, the mass-volume ratio of sodium alginate to ultrapure water in step S103 is 1:100, the concentrations of lipase and protease are 100 U / mL and 50 U / mL respectively, the concentration of calcium chloride solution in step S104 is 0.2 M, the mass-volume ratio of hyaluronic acid to ultrapure water in step S105 is 1:50, the concentrations of lipase and protease are 100 U / mL and 50 U / mL respectively, the mass ratio of hyaluronic acid, poly-N-isopropyl acrylamide and ammonium persulfate is 1:0.5:0.05, the concentration of superoxide dismutase is 1000 U / mL, the mass-volume ratio of hyaluronic acid, chitosan and ultrapure water in step S106 is 3:2:100, the concentration of hyaluronidase is 50 U / mL, and the mass ratio of the multilayer microcapsule containing ginseng essential oil, the surface layer of fast-release layer material, the middle layer of slow-release layer material and the deep layer of controlled-release layer material in step S107 is 2:3:2:

1. ​

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