Preparation method and application of cistanche extract

Abstract

The application discloses a preparation method and application of a cistanche extract, and relates to the technical field of skin care products containing the cistanche extract. In view of the problems of low extraction efficiency, easy degradation of active ingredients of traditional cistanche, and poor compatibility and insufficient stability of existing skin care products for inhibiting melanin, the preparation method is characterized by optimizing raw material pretreatment, ultrasonic extraction, vacuum concentration and spray drying process, and efficiently retaining active ingredients. The skin care product containing the extract and specific active antioxidant ingredients is also disclosed. The application improves the extraction efficiency and ingredient retention rate, and has good stability, excellent and mild melanin inhibition effect, controllable process, and is suitable for large-scale production, and meets the demand for natural and efficient whitening.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care products containing Cistanche extract, and in particular to a preparation method and application of Cistanche extract. BACKGROUND

[0002] As a traditional medicinal plant in China, Cistanche is rich in phenylethanoid glycosides (such as scrophulariside, verbascoside), polysaccharides, flavonoids and other active ingredients, and has excellent antioxidant, anti-inflammatory and cell protection effects. With the growing demand for natural and mild whitening skin care products, inhibiting melanin production has become the core direction of whitening skin care research. Excessive deposition of melanin is the main cause of skin discoloration and uneven skin tone. Traditional whitening ingredients such as hydroquinone and retinoids can inhibit melanin synthesis, but they have strong irritation, low skin tolerance, and can damage the skin barrier when used for a long time. Natural plant extracts have gradually become an important choice to replace traditional ingredients due to their high safety and mild efficacy.

[0003] However, the current preparation technology of Cistanche extract still has significant bottlenecks. Traditional extraction methods mostly use conventional soaking or single reflux extraction, which has the problems of low extraction efficiency and poor retention rate of active ingredients: either the degradation of heat-sensitive components such as phenylethanoid glycosides due to excessive extraction temperature or improper solvent concentration; or insufficient dissolution of effective components due to insufficient extraction times and unbalanced solid-liquid ratio. In the separation and purification process, traditional processes often use coarse filtration, which easily leaves impurities, and subsequent atmospheric concentration further exacerbates the loss of active ingredients. In the drying process, the extraction is often dried, which easily causes the extraction to caking and the effective components to inactivate, seriously affecting its application effect in skin care products.

[0004] At the same time, the existing melanin-inhibiting skin care products containing plant extracts also face formulation problems: the compatibility of the extract with the oil phase and emulsifier is poor, which easily causes separation and precipitation, resulting in insufficient product stability; the active ingredients are easily oxidized during storage, and excessive preservatives need to be added, which increases the risk of skin irritation and is difficult to meet the needs of consumers for "high efficiency, mildness and stability" whitening products. In summary, developing an extraction process that efficiently retains the active ingredients of Cistanche and a mild melanin-inhibiting skin care product formulation that is suitable for the active ingredients is the key to breaking through the current technical bottlenecks. SUMMARY

[0005] In view of the low preparation efficiency of Cistanche extract, poor retention rate of active ingredients in the prior art, and the problems of poor formula compatibility and insufficient stability of existing melanin inhibiting skin care products, the present application provides a preparation method of Cistanche extract which can efficiently extract and fully retain active ingredients of Cistanche, and has controllable process parameters and high stability. At the same time, the present application provides a melanin inhibiting skin care product which takes the extract as the core active ingredient, is compatible with a new specific active antioxidant ingredient and a mild auxiliary material, has excellent melanin inhibiting effect, good skin tolerance and good product stability, so as to meet the natural, mild and efficient whitening skin care needs and break through the technical bottleneck of the current Cistanche extraction process and related skin care product formula.

[0006] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows: a preparation method of Cistanche extract, comprising the following steps:

[0007] (1) Raw material pretreatment: select dry Cistanche, clean after removing impurities;

[0008] (2) Extraction: crush the pretreated Cistanche, add an extraction solvent, and extract 3-5 times at 20-80℃, with each extraction time being 3-4h, and combine the extraction liquid;

[0009] (3) Separation and purification: filter the combined extraction liquid to remove the filter residue and obtain a clear extraction liquid; concentrate the clear extraction liquid to obtain a concentrated liquid;

[0010] (4) Drying: dry the concentrated liquid to obtain Cistanche extract.

[0011] Further, the moisture content of the dry Cistanche in step (1) is ≤15%.

[0012] Further, the particle size of the crushed Cistanche is 1-10mm.

[0013] Further, in step (2), the extraction solvent is an ethanol aqueous solution with a volume fraction of 80%-95%.

[0014] Further, in step (2), the mass-volume ratio of Cistanche to extraction solvent is 1:5-1:20 (g / mL).

[0015] Further, in step (2), the extraction method is ultrasonic extraction, and the power of ultrasonic extraction is 300-500W and the frequency is 30-50kHz.

[0016] Further, the filtration is performed using filter cloth or microporous filter membrane with a pore size of 0.22-5μm; the concentration is performed using vacuum concentration, the vacuum degree is -0.06 to -0.1MPa, the concentration temperature is 40-60℃, and the concentration is performed to a solid content of 20%-50% in the concentrated liquid.

[0017] Further, in the step (4), the drying method is spray drying, wherein the inlet temperature of the spray drying is 160-220℃, and the outlet temperature is 80-120℃.

[0018] Application of cistanche extract in preparation of skin care product for inhibiting melanin.

[0019] A skin care product for inhibiting melanin is prepared from the following components: cistanche extract 4-9 parts, active antioxidant ingredient 1-3 parts, oil phase component 8-20 parts, emulsifier 2-5 parts, humectant 5-15 parts, thickening agent 0.1-1 part, preservative 0.1-0.8 part, and deionized water added to 100 parts.

[0020] The active antioxidant ingredient is a structure shown in formula 1:

[0021] The structure of formula 1 is:

[0022] R1 in formula 1 is a substituent selected from one or more of the following: fluorine, trifluoromethyl, nitro,

[0023] The * in the substituent is a linking site.

[0024] Further, the active antioxidant ingredient is one or more of the following compounds shown in the structure:

[0025]

[0026]

[0027] Further, the oil phase component is selected from one or more of the following: jojoba seed oil, squalane, almond oil, shea butter oil, ethylhexyl palmitate.

[0028] Further, the emulsifier is one or more of the following: cetearyl olivate, sorbitol olivate, polyglyceryl-3-diisostearate, glyceryl stearate, PEG-100 stearate, cetearyl alcohol, cetearyl glucoside.

[0029] Further, the humectant is selected from one or more of the following: glycerol, butylene glycol, propylene glycol, sodium hyaluronate, panthenol, sodium pyrrolidone carboxylate, betaine, xylityl glucoside.

[0030] Further, the thickening agent is selected from one or more of the following: xanthan gum, carrageenan, microcrystalline cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, sodium carboxymethyl cellulose.

[0031] ​​​​​​Further, the preservative is selected from one or more of phenoxyethanol, potassium sorbate, sodium benzoate, ethylhexylglycerin, 1,2-hexanediol, 1,2-pentanediol.

[0032] The application provides a high-efficiency and stable preparation method of Cistanche extract, which extracts and retains heat-sensitive active ingredients such as echinacoside, verbascoside and polysaccharide by adopting ultrasonic technology with a power of 300-500 W and a frequency of 30-50 kHz, combining with an ethanol aqueous solution with a volume fraction of 80%-95% as a solvent, and extracting 3-5 times (3-4 h each time) at 20-80 DEG C; the extraction efficiency and economy are significantly improved by strictly controlling the moisture content (≤15%) and the broken particle size (1-10 mm) of raw materials and optimizing the solid-liquid ratio (1:5-1:20 g / mL); then, impurities are removed by filtering through a microporous filter membrane (pore size 0.22-5 mu m), and the extract liquid is accurately concentrated to 20%-50% by vacuum low-temperature concentration (vacuum degree -0.06 to -0.1 MPa, temperature 40-60 DEG C), so that the degradation of heat-sensitive ingredients is effectively avoided; finally, a powder product with good solubility and high activity is instantaneously obtained by spray drying (air inlet temperature 160-220 DEG C, air outlet temperature 80-120 DEG C). The whole process parameters are clear and controllable, have good reproducibility, are suitable for large-scale production, and completely solve the problems of low extraction efficiency, easy degradation of ingredients and unstable quality in the traditional method, thereby providing reliable guarantee for the application of high-quality Cistanche extract in the field of skin care products and the like.

[0033] The active antioxidant ingredient has a structure in which a hydroxyl group can provide a hydrogen atom, neutralize free radicals and interrupt lipid peroxidation chain reactions, thereby protecting skin cells from oxidative damage; meanwhile, the structure can competitively bind with tyrosinase and inhibit the catalytic activity thereof, thereby reducing the synthesis of melanin from the source and improving uneven skin color and spot problems. In the melanin inhibition skin care product formula, the active antioxidant ingredient (1-3 parts) is compounded with Cistanche extract (4-9 parts), and a significant synergistic effect is generated. The Cistanche extract is rich in phenethyl glycoside and polysaccharide and the like, which can provide auxiliary anti-inflammatory and moisturizing effects and improve the skin barrier, while the active antioxidant ingredient focuses on precisely attacking oxidative stress and melanin activation signal pathways at the molecular level, so as to jointly build a multi-level and multi-target whitening antioxidant system, and finally make the product have excellent efficacy, high safety and good stability.

[0034] Compared with the prior art, the application has the following beneficial effects:

[0035] 1. Extraction process is more optimal: Compared with the traditional Cistanche extraction method, the present application can improve the extraction efficiency of active ingredients, better retain heat-sensitive active ingredients, solve the problems of low extraction efficiency, easy degradation of ingredients, and unstable product quality in traditional process, and the process parameters are controllable, which is more suitable for large-scale production, by optimizing the pretreatment of raw materials, extraction parameters (such as solvent, ultrasonic conditions) and subsequent separation and drying process.

[0036] 2. Skin care product formula is more reasonable: Compared with existing skin care products containing plant extracts for inhibiting melanin, the skin care product prepared by the present application can improve the compatibility of the extract and other components, reduce problems such as stratification and precipitation, improve product stability, and reduce the risk of skin irritation caused by adding excessive preservatives, by compounding the specific active antioxidant ingredients with the Cistanche extract, and adding mild auxiliary materials.

[0037] 3. Product efficacy is more comprehensive and mild: Compared with traditional whitening ingredients or single-effect skin care products, the product of the present application has better effect on inhibiting melanin, and the Cistanche extract and the specific active antioxidant ingredients have synergistic effect, and have skin protection effects such as anti-inflammatory and moisturizing, and are more mild, which can meet the natural, mild and efficient whitening and skin care needs, and avoid the problems of strong irritation and low skin tolerance of traditional whitening ingredients. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 The nuclear magnetic resonance spectrum of the active antioxidant ingredient 1 of the present application.

[0039] Figure 2 The nuclear magnetic resonance spectrum of the active antioxidant ingredient 5 of the present application. DETAILED DESCRIPTION

[0040] The technical solutions of the present application will be described in detail below with reference to the drawings in the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0041] Preparation Example 1

[0042] Preparation of active antioxidant ingredient 1:

[0043] .

[0044] Under a nitrogen atmosphere, 15.00 g of compound 1, 12.93 g of compound 2, 43.99 g of cesium carbonate, 0.64 g of CuI, and 180 mL of DMSO were added sequentially to the reaction system, and the mixture was stirred at 140 °C for 24 hours. After the reaction was complete, the reaction solution was filtered through diatomaceous earth, and the filtrate was evaporated to dryness under negative pressure. The filtrate was then purified by silica gel column chromatography (using a mixture of n-heptane and ethyl acetate as the eluent), and evaporated to dryness again under negative pressure to obtain 18.80 g of compound 3. Mass spectrometry M / Z: 346 [MS+1].

[0045] Under a nitrogen atmosphere, 18.80 g of compound 3, 12.97 g of compound 4, 15.05 g of potassium carbonate, 3.16 g of tetrakis(triphenylphosphine)palladium, and 220 mL of xylene were added sequentially to the reaction system. The mixture was stirred at 110 °C for 12 hours. After the reaction was complete, the reaction solution was filtered through diatomaceous earth. The filtrate was evaporated to dryness under negative pressure and purified by silica gel column chromatography (using a mixture of n-heptane and ethyl acetate as the eluent). The purified filtrate was then evaporated to dryness under negative pressure to obtain 23.02 g of the active antioxidant component 1. Mass spectrometry M / Z: 508 [MS+1], NMR spectroscopy [see details]. Figure 1 .

[0046] Preparation Examples 2-6

[0047] In Preparation Examples 2-6, active antioxidant components 2-6 were prepared sequentially, following the preparation method of Preparation Example 1, except that compound 2 was replaced. The rest remained the same as in Example 1. For details, please refer to Table 1.

[0048] Table 1.

[0049]

[0050] Example 1

[0051] Preparation of a skincare product that inhibits melanin:

[0052] 1. Raw material ratio:

[0053] Cistanche deserticola extract: 7 parts;

[0054] Active antioxidant component (using active antioxidant component 1 prepared in Preparation Example 1): 2 parts;

[0055] Oil phase component (jojoba seed oil): 12 parts, purchased from: Hubei Chenghai Chemical Co., Ltd.;

[0056] Emulsifier (cetearyl oleate): 3 parts, purchased from Shanghai Wencai New Material Technology Co., Ltd.;

[0057] Moisturizer (glycerin and sodium hyaluronate, mass ratio 4:1): 10 parts; glycerin was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; sodium hyaluronate was purchased from Changzhou Wobang Biotechnology Co., Ltd.

[0058] Thickener (xanthan gum): 0.5 parts, purchased from Shandong Fengtai Biotechnology Co., Ltd.;

[0059] Preservative (phenoxyethanol): 0.3 parts, purchased from: Liaoning Kelon Fine Chemical Co., Ltd.;

[0060] Deionized water: 65.2 parts.

[0061] 2. Preparation method:

[0062] 2.1 Preparation of Cistanche deserticola extract:

[0063] (1) Raw material pretreatment: Select dried Cistanche deserticola raw material, dry it until its moisture content is ≤15%, remove impurities such as mud and stones contained therein, and wash it clean with water or deionized water, and drain it for later use.

[0064] (2) Extraction: The pretreated Cistanche deserticola was mechanically crushed, and the particle size after crushing was between 1-10 mm. A 90% ethanol aqueous solution was used as the extraction solvent. The mass-volume ratio of Cistanche deserticola raw material to extraction solvent was 1:15 (g / mL). Ultrasonic-assisted extraction was performed at 80℃ with an ultrasonic power of 500W and a frequency of 50kHz. Under these conditions, five extractions were performed, each lasting 4 hours. The extracts from all extractions were combined to obtain the total extract.

[0065] (3) Separation and purification: The combined extracts were filtered to completely remove insoluble particles and filter residue from the solution. Filtration was performed using a filter cloth with a pore size of 5 μm to obtain a clear extract. The clear extract was then concentrated under vacuum. The concentration process was carried out at a vacuum of -0.1 MPa and a temperature of 50 °C. Concentration was stopped when the solid content of the concentrate was 50%, and the concentrate was obtained.

[0066] (4) Drying: The concentrate is spray-dried, with the inlet air temperature controlled at 160℃ and the outlet air temperature controlled at 100℃ to obtain Cistanche deserticola extract powder.

[0067] 2.2 Preparation of a skin care product that inhibits melanin:

[0068] (1) Preparation of oil phase: Add the oil phase components jojoba seed oil and emulsifier cetearyl oleate to the oil phase pot, heat to 80°C, stir until completely dissolved and keep warm;

[0069] (2) Preparation of aqueous phase: Deionized water, humectant, thickener xanthan gum are added to the aqueous phase pot, heated to 85°C, stirred until completely dissolved and kept warm;

[0070] (3) Emulsification: Slowly add the oil phase to the water phase, homogenize and emulsify for 5 minutes (homogenizer speed 3000r / min), and then keep warm and stir for 15 minutes;

[0071] (4) Cooling and adding: Cool the system to below 45°C, add Cistanche deserticola extract, active antioxidant 1 and preservative, and stir well;

[0072] (5) Adjustment and discharge: Adjust the pH to 6.0, continue stirring until completely uniform, and discharge to obtain the melanin-inhibiting skin care product.

[0073] Examples 2-6

[0074] A skin care product for inhibiting melanin was prepared by referring to the preparation method of Example 1, except that the active antioxidant ingredients were replaced sequentially with active antioxidant ingredients 2-6, and the rest remained the same as in Example 1.

[0075] Comparative Example 1

[0076] A method for preparing a skin care product that inhibits melanin production involves replacing the active antioxidant ingredient with α-arbutin, while keeping the rest the same as in Example 1.

[0077] Comparative Example 2

[0078] A method for preparing a skin care product that inhibits melanin production involves replacing the active antioxidant ingredient with niacinamide, while keeping the rest the same as in Example 1.

[0079] Comparative Example 3

[0080] A skin care product for inhibiting melanin was prepared by replacing the active antioxidant ingredient with comparative compound 1, while the rest remained the same as in Example 1.

[0081] Comparative compound 1: .

[0082] Comparative Example 4

[0083] A skin care product for inhibiting melanin was prepared by replacing the active antioxidant ingredient with comparative compound 2, while the rest remained the same as in Example 1.

[0084] Comparative compound 2: .

[0085] Comparative Example 5

[0086] A method for preparing a skin care product that inhibits melanin production, without adding any active antioxidant ingredients, is otherwise identical to that in Example 1.

[0087] Comparative Example 6

[0088] A method for preparing a skin care product that inhibits melanin involves replacing 90% of the aqueous ethanol solution in the preparation of Cistanche deserticola extract with a 60% aqueous ethanol solution, while keeping the rest the same as in Example 1.

[0089] Comparative Example 7

[0090] The preparation of a skin care product that inhibits melanin involves replacing the concentration temperature in the method for preparing Cistanche deserticola extract with 80°C, while keeping the rest the same as in Example 1.

[0091] Comparative Example 8

[0092] A skin care product for inhibiting melanin was prepared without adding Cistanche deserticola extract, and otherwise remained the same as in Example 1.

[0093] Performance testing:

[0094] 1. The effect of a skincare product that inhibits melanin production on melanin levels:

[0095] Set up a blank control group, an example group, and a comparative group, with at least 3 replicates in each group. The blank control group (containing cells, with only 2 mL of complete culture medium added) was also included.

[0096] Cell seeding: After resuscitating the cells (human malignant melanoma cells MNT-1), passage them for one generation. When the confluence of the passaged cells reaches 70%-80%, plate them. Seed the cells evenly into 6-well plates, 2 mL per well, and incubate overnight in an incubator (37℃, 5% CO2).

[0097] Medium replacement and drug administration: When the cell confluence in the well plate reaches 40%-60% under a microscope, discard the old culture medium and replace the medium for each group. Add 2 mL of culture medium to each well of the blank control group; add 2 mL of culture medium containing the corresponding concentration of kojic acid to each well of the positive control group; add 2 mL of culture medium containing the corresponding concentration of the sample to each well of the sample group. After loading, incubate the 6-well plate in an incubator (37℃, 5% CO2) for 48 h.

[0098] Cell collection: Prepare the water bath to 80°C and pre-cool the cells with PBS buffer beforehand. Then, digest the cells in the wells with trypsin, centrifuge, discard the supernatant, and collect the cells into new EP tubes. Wash twice with pre-cooled PBS to terminate the drug reaction.

[0099] Cell lysis: After washing, add 200 μL of 1M NaOH to each tube, then place the EP tube on a foam buoy for water bath heating for 10 min, until the melanin is completely dissolved.

[0100] Melanin absorbance measurement: 100 μL of lysis buffer was taken from each tube and transferred to a 96-well plate, and the OD value was read at 405 nm.

[0101] Result calculation:

[0102] Melanin content (%) = (Sample melanin content value OD / Blank melanin content OD value);

[0103] Melanin inhibition rate (%) = (blank control group - sample group) / blank control group.

[0104] The data is shown in Table 2.

[0105]

[0106] The blank control group, serving as a baseline, exhibited the highest melanin content and the lowest inhibition rate. The example groups (using the Cistanche deserticola extraction process and specific active antioxidant components of this invention) generally showed a trend of significantly lower melanin content and significantly higher melanin inhibition rate than most comparative groups, indicating that the present invention has a superior effect in inhibiting melanin production.

[0107] Comparison of different comparative examples shows that the group in which the active antioxidant ingredient of the present invention was replaced with a traditional whitening ingredient (such as α-arbutin or niacinamide) had a significantly weaker melanin inhibition effect than the example group, demonstrating the advantage of the specific active antioxidant ingredient of the present invention. The group in which a comparative compound with a non-inventory structure was used to replace the active antioxidant ingredient showed a further weakening effect, indicating that the specific structure of the active antioxidant ingredient of the present invention is crucial to its efficacy. The group in which no active antioxidant ingredient or no Cistanche deserticola extract was added showed a significant decrease in melanin inhibition effect, reflecting the synergistic effect of the combination of Cistanche deserticola extract and the specific active antioxidant ingredient. The group in which the Cistanche deserticola extraction process was changed (such as adjusting the ethanol concentration or concentration temperature) also showed a less effective result than the example group, highlighting the necessity of the Cistanche deserticola extraction process of the present invention in ensuring the efficacy of the final product.

[0108] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A skincare product that inhibits melanin production, characterized in that, It is made from the following components in parts by weight: 4-9 parts of Cistanche deserticola extract, 1-3 parts of active antioxidant, 8-20 parts of oil phase, 2-5 parts of emulsifier, 5-15 parts of moisturizer, 0.1-1 part of thickener, 0.1-0.8 parts of preservative, and deionized water to 100 parts; The preparation method of the Cistanche deserticola extract includes the following steps: (1) Raw material pretreatment: Select dried Cistanche deserticola, remove impurities and wash it clean; (2) Extraction: Crush the pretreated Cistanche deserticola, add the extraction solvent, and extract 3-5 times at 20-80℃, with each extraction time being 3-4 hours. Combine the extracts. (3) Separation and purification: Filter the combined extracts to remove the filter residue and obtain a clear extract; concentrate the clear extract to obtain a concentrated extract; (4) Drying: The concentrate was dried to obtain Cistanche deserticola extract; The active antioxidant component has the structure shown in Formula 1: The structure of Equation 1 is as follows: ; In Formula 1, R1 is a substituent selected from: fluorine, trifluoromethyl, nitro, ... , , One or more of the following; The asterisk (*) in the substituents represents a linking site.

2. The skincare product for inhibiting melanin according to claim 1, characterized in that, The moisture content of the dried Cistanche deserticola in step (1) is ≤15%.

3. A skincare product for inhibiting melanin according to claim 1, characterized in that, In step (2), the particle size of the crushed Cistanche deserticola is 1-10 mm; In step (2), the extraction solvent is an aqueous ethanol solution with a volume fraction of 80%-95%; In step (2), the mass-to-volume ratio of Cistanche deserticola to the extraction solvent is 1:5-1:20 (g / mL); In step (2), the extraction method is ultrasonic extraction, with a power of 300-500W and a frequency of 30-50kHz.

4. A skincare product for inhibiting melanin according to claim 1, characterized in that, In step (3), filtration is performed using filter cloth or microporous membrane with a pore size of 0.22-5 μm; concentration is performed using vacuum concentration with a vacuum degree of -0.06 to -0.1 MPa and a concentration temperature of 40-60℃, until the solid content of the concentrate is 20%-50%. In step (4), the drying method is spray drying, wherein the inlet air temperature of spray drying is 160-220℃ and the outlet air temperature is 80-120℃.

5. A skincare product for inhibiting melanin according to claim 1, characterized in that, The oil phase component is selected from one or more of the following: jojoba seed oil, squalane, almond oil, shea butter, and ethylhexyl palmitate.

6. A skincare product for inhibiting melanin according to claim 1, characterized in that, The emulsifier is selected from one or more of the following: cetearyl oleate, sorbitan oleate, polyglycerol-3-diisostearate, glyceryl stearate, PEG-100 stearate, cetearyl alcohol, and cetearyl glucoside.

7. A skincare product for inhibiting melanin according to claim 1, characterized in that, The moisturizer is selected from one or more of glycerin, butylene glycol, propylene glycol, sodium hyaluronate, panthenol, sodium pyrrolidone carboxylate, betaine, and xylitol glucoside; The thickener is selected from one or more of the following: xanthan gum, carrageenan, microcrystalline cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and sodium carboxymethyl cellulose; The preservative is selected from one or more of the following: phenoxyethanol, potassium sorbate, sodium benzoate, ethylhexylglycerin, 1,2-hexanediol, and 1,2-pentanediol.

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