Traditional Chinese medicine formula for promoting blood circulation and removing toxicity and preparation method of nanogel of traditional Chinese medicine formula

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a blood-activating and detoxifying traditional Chinese medicine formula and a nanogel preparation method thereof. The invention provides a traditional Chinese medicine formula for promoting blood circulation and removing toxicity and a nanogel preparation method of the traditional Chinese medicine formula for promoting blood circulation and removing toxicity to solve the problem that in the prior art, a good external application traditional Chinese medicine formula capable of being used for skin with damp-heat toxin accumulation does not exist, and the traditional Chinese medicine formula for promoting blood circulation and removing toxicity and the nanogel preparation method of the traditional Chinese medicine formula for promoting blood circulation and removing toxicity comprise monarch drugs, ministerial drugs, adjuvant drugs and conductant drugs, the ministerial drugs comprise honeysuckle flowers, fructus forsythiae, paris polyphylla, cortex dictamni and fructus kochiae, the adjuvant drugs comprise radix salviae miltiorrhizae, flos carthami, garden balsam stems and centella asiatica, and the conductant drug comprises bletilla striata. The traditional Chinese medicine composition provided by the invention is precise and appropriate in compatibility and distinct in gradation, and can form a synergistic treatment chain of clearing heat, detoxifying, activating blood and promoting tissue regeneration, so that the wound healing speed of the skin surface is accelerated, and the traditional Chinese medicine composition has a relatively good curative effect on damp-heat and toxic stasis type skin diseases.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, and in particular relates to a traditional Chinese medicine formula for promoting blood circulation and detoxifying, and a method for preparing its nanogel. Background Technology

[0002] The accumulation of damp-heat toxins in the skin is a common pathogenesis in Traditional Chinese Medicine (TCM) dermatology, frequently seen in acute / subacute eczema, contact dermatitis, drug-induced dermatitis, impetigo, skin ulcers, and carbuncles. These conditions are characterized by skin erythema, swelling, exudation, erosion, itching, and pain, often accompanied by infection and prone to recurrence. Modern medicine primarily uses corticosteroids, antihistamines, and antibiotics for treatment. While these can provide short-term symptom relief, they carry risks such as increased drug resistance, high recurrence rates, and long-term use leading to skin atrophy and systemic side effects. Therefore, safe and effective alternative therapies are urgently needed.

[0003] For example, a Chinese invention patent application discloses a traditional Chinese medicine compound preparation for detoxification, anti-cancer, blood circulation and stasis-removing and its uses [application number: 201810125596.6]. This invention patent application is made from the following raw materials in parts by weight: 1-30 parts of cat ginseng, 1-30 parts of rock hemp, 1-30 parts of mountain ash mushroom, and 1-15 parts of chicken gizzard lining.

[0004] Although this invention patent application has the advantage of being applicable to the treatment of malignant tumors, including digestive tract tumors such as liver cancer, intestinal cancer, and stomach cancer, as well as lung cancer, it still cannot be applied to the skin surface for promoting blood circulation and detoxification. Summary of the Invention

[0005] The purpose of this invention is to address the above-mentioned problems by providing a traditional Chinese medicine formula for promoting blood circulation and detoxifying.

[0006] Another objective of this invention is to address the aforementioned problems by providing a method for preparing nanogels from traditional Chinese medicine formulas for promoting blood circulation and detoxification.

[0007] To achieve the above objectives, the present invention adopts the following technical solutions:

[0008] A traditional Chinese medicine formula for promoting blood circulation and detoxifying includes principal herbs, assistant herbs, adjuvant herbs, and guiding herbs. The principal herbs include Phellodendron bark, charred gardenia fruit, and dried alum. The assistant herbs include honeysuckle, forsythia fruit, Paris rhizome, Dictamnus root bark, and Kochia fruit. The adjuvant herbs include Salvia miltiorrhiza, safflower, Clematis chinensis, and Centella asiatica. The guiding herbs include Bletilla striata.

[0009] Phellodendron bark: Bitter and cold in nature. It enters the kidney and bladder meridians. It is the main ingredient in the formula for clearing heat and drying dampness, especially good at clearing damp-heat in the lower burner (including the skin), purging fire and detoxifying. It targets the core pathogenesis of "damp-heat toxin".

[0010] Charred Gardenia: Bitter and cold in nature. It enters the Heart, Lung, and Triple Burner meridians. After being charred, Gardenia's cold nature is slightly reduced, making it more effective in clearing heat and dampness, cooling the blood, and detoxifying. It can clear heat from the Triple Burner, especially good at clearing heart fire and relieving stagnant heat, assisting Phellodendron bark in clearing heat, drying dampness, and detoxifying, and also cooling the blood.

[0011] Alum: Sour, astringent, and cold in nature. It enters the lung, spleen, liver, and large intestine meridians. Externally, it has significant effects in astringing dampness and healing sores, detoxifying, killing parasites, and relieving itching; internally (in small amounts), it also has the effects of drying dampness, stopping bleeding, and stopping diarrhea. In this formula, its powerful dampness-astringing, antipruritic, and sore-healing effects are crucial for exudation, itching, and erosion caused by damp-heat.

[0012] The above three herbs can clear heat (purge fire), dry dampness, and detoxify, setting the tone for the overall treatment.

[0013] Honeysuckle: Sweet and cold in nature. It enters the lung, heart, and stomach meridians. It clears heat and detoxifies, and disperses wind-heat. Its heat-clearing and detoxifying power is strong, and its medicinal properties are relatively mild. It can help the principal herb enhance its detoxifying and swelling-reducing effects, especially for carbuncles and boils caused by heat toxins.

[0014] Forsythia: Bitter and slightly cold in nature. It enters the lung, heart, and small intestine meridians. It clears heat and detoxifies, reduces swelling and dissipates nodules, and disperses wind-heat. It is known as the "holy medicine for sores". When used with honeysuckle, it enhances the effects of detoxification, dissipation of nodules and reduction of swelling, and also helps to clear heart fire.

[0015] Paris polyphylla (also known as Paris polyphylla): Bitter and slightly cold in nature. It enters the liver meridian. It clears heat and detoxifies, reduces swelling and relieves pain, cools the liver and calms the nerves. It has strong detoxifying power, especially effective for carbuncles, boils, and snake bites. It also cools the blood, helping the principal herb to penetrate deeper for detoxification, swelling reduction, and pain relief.

[0016] Dictamnus dasycarpus root bark: Bitter and cold in nature. It enters the spleen, stomach, and bladder meridians. It clears heat and dries dampness, dispels wind and detoxifies, and relieves itching. It is an essential medicine for treating damp-heat sores, urticaria, scabies, and pruritus, and can assist the principal medicine in drying dampness and relieving itching, while also dispelling wind.

[0017] Kochia scoparia: Its properties are bitter, pungent, and cold. It enters the kidney and bladder meridians. It clears heat and promotes diuresis, dispels wind and relieves itching. It promotes urination and relieves strangury, allowing damp-heat to be eliminated through urination; its effect in dispelling wind and relieving itching is excellent, especially for itchy skin.

[0018] The above five herbs can assist the principal herb, enhance its effects of clearing heat and detoxifying, drying dampness and relieving itching, reducing swelling and dissipating nodules, and introduce the treatment methods of dispelling wind and promoting diuresis to guide dampness downward, thus expanding the scope of treatment (targeting itching, wind-evil, difficulty in urination, etc.).

[0019] Salvia miltiorrhiza: Bitter and slightly cold in nature. It enters the heart and liver meridians. It invigorates blood circulation, removes blood stasis, cools the blood and reduces swelling, relieves irritability and calms the mind. For the concurrent symptoms of "blood stasis" caused by damp-heat and toxic evil obstructing qi and blood, it can invigorate blood circulation to remove blood stasis to reduce swelling and relieve pain, cool the blood to help clear heat, and promote the dissipation or healing of sores.

[0020] Safflower: Pungent and warm in nature. It enters the heart and liver meridians. It invigorates blood circulation, regulates menstruation, disperses blood stasis, and relieves pain. It assists Salvia miltiorrhiza in enhancing its blood-activating and stasis-removing effects, promoting local blood circulation, dispersing stagnation, and alleviating swelling and pain.

[0021] Chinese Speranskia Root: Pungent and warm in nature, attributing to Liver and Kidney meridians. Expelling wind and removing dampness, relaxing muscles and activating blood circulation, detoxifying and relieving pain. It can penetrate deep into the muscles and meridians to dispel wind-dampness, assisting the monarch and ministerial drugs to remove dampness and relieve itching, activate blood circulation and relieve pain, especially effective for stubborn skin itching and joint discomfort.

[0022] Centella Asiatica: Bitter, pungent and cold in nature, attributing to Liver, Spleen and Kidney meridians. Clearing heat and promoting diuresis, detoxifying and detumescing, activating blood circulation and relieving pain, promoting wound healing. Modern research has confirmed its good effect in promoting the repair of skin trauma, assisting the monarch and ministerial drugs to detoxify and detumesce, generate flesh and astringe sores.

[0023] The above four herbs can play the roles of resolving the concurrent syndrome of blood stasis (Salvia miltiorrhiza, Carthamus tinctorius, Chinese Speranskia Root), promoting the healing of ulcers / surface repair of skin (Salvia miltiorrhiza, Centella Asiatica), enhancing the expelling of wind-dampness and dredging collaterals to relieve pain (Chinese Speranskia Root), and using the mutual restraint of cold and hot medicinal properties (the warmth of Carthamus tinctorius and Chinese Speranskia Root to assist in restraining cold drugs).

[0024] Bletilla striata: Bitter, sweet, astringent and slightly cold in nature, attributing to Lung, Liver and Stomach meridians. Astringing to stop bleeding, detumescing and generating flesh. In this formula, it mainly takes the effect of generating flesh and astringing sores to promote the healing of sore surfaces and skin breaks. Its sticky nature can also harmonize various drugs. As the guiding drug, its astringent and flesh-generating characteristics guide the medicinal effects to the skin lesions that need to be repaired.

[0025] In the above-mentioned traditional Chinese medicine formula for activating blood circulation and detoxification, the traditional Chinese medicine formula includes the following components in parts by weight:

[0026] Phellodendron amurense Rupr. 6 - 12 parts; [[ID=​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​​

[0040] 10 portions of Coriander bark;

[0041] 10 portions of roasted gardenia;

[0042] 5 parts of calcined alum;

[0043] 8 portions of honeysuckle;

[0044] Forsythia suspensa 8 portions;

[0045] Two portions of Paris polyphylla;

[0046] Four parts of Dictamnus dasycarpus root bark;

[0047] Kochia scoparia fruit, 8 parts;

[0048] 10 portions of Salvia miltiorrhiza;

[0049] 5 parts safflower;

[0050] 4 parts of Clematis armandii;

[0051] 8 portions of Centella asiatica;

[0052] 5 parts of Bletilla striata.

[0053] A method for preparing the above-mentioned blood-activating and detoxifying traditional Chinese medicine formula nanogel includes the following steps:

[0054] Step 1: Place the following herbs in a container: Phellodendron bark, charred gardenia fruit, dried alum, honeysuckle flower, forsythia fruit, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza root, safflower flower, Clematis chinensis root, Centella asiatica root, and Bletilla striata root. Add water to cover the herbs and soak at room temperature for 80-120 minutes. Heat to a boil and maintain boiling for 20-40 minutes. After solid-liquid separation, obtain the first decoction. Add water to the dregs and heat to a boil, maintaining boiling for 20-40 minutes. After solid-liquid separation, obtain the second decoction and the dregs. Combine the first and second decoctions, mix them, and concentrate to obtain the medicinal liquid for later use.

[0055] Step 2: Mix the Chinese herbal residue obtained in Step 1 with PEG800 in a container and heat to 90-110℃. Then, use a high-speed shearing machine to shear and filter to obtain 200-400nm nano-sized Chinese herbal powder.

[0056] Step 3: Combine the herbal liquid obtained in Step 1, the nano-herbal powder obtained in Step 2, water, ethanol, Jema BP, silica and chitosan. First, stir with a mixer at 200-400 rpm for 40-80 minutes, and then emulsify with a homogenizer at 10000-12000 rpm for 60-80 minutes to obtain the herbal nano-coated stock solution.

[0057] Step 4: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 3. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. Stir for more than 30 minutes, then add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

[0058] In the above-mentioned method for preparing nanogels, in step one, water is added to cover the medicinal materials placed in the container by 2-5 cm.

[0059] In the above-mentioned method for preparing nanogels, in step one, water is added to the residue to cover it by 0.5-2 cm, and the temperature of the added water is 30-50℃.

[0060] In the above-mentioned method for preparing nanogels, the mass ratio of the Chinese herbal medicine residue to PEG800 in step two is 1:1.

[0061] In the above-mentioned method for preparing nanogels, the shearing step in step two is as follows: first, shear at a rotation speed of 600-1000 r / min for 40-80 min, and then increase the shearing speed to 4500-5000 r / min for 60-120 min.

[0062] In the above-mentioned preparation method of nanogel, in step three, 70 parts by weight of traditional Chinese medicine liquid, 10 parts by weight of nano-traditional Chinese medicine powder, 4 parts by weight of water, 10 parts by weight of ethanol, 0.5 parts by weight of Jema BP, 0.5 parts by weight of silica and 5 parts by weight of chitin are added for compounding.

[0063] In the above-mentioned method for preparing nanogels, the particle size of silica in step three is 20 nm.

[0064] Compared with existing technologies, the advantages of this invention are:

[0065] 1. The traditional Chinese medicine formula provided by this invention is well-matched and has distinct layers, which can form a synergistic treatment chain of clearing heat, detoxifying, promoting blood circulation and promoting tissue regeneration, thereby accelerating the healing speed of wounds on the skin surface and having a good curative effect on skin diseases of the damp-heat and toxic stasis type.

[0066] 2. The preparation method provided by this invention is simple, and the obtained nanogel can be directly applied to the affected area, which is convenient to use and has a fast effect. Detailed Implementation

[0067] The present invention will now be described in further detail with reference to specific embodiments.

[0068] Example 1

[0069] This embodiment provides a traditional Chinese medicine formula for promoting blood circulation and detoxification, and a method for preparing its nanogel, comprising the following components by weight:

[0070] 12 portions of Coriander bark;

[0071] Six portions of roasted gardenia fruit;

[0072] 3 parts of calcined alum;

[0073] Honeysuckle 6 portions;

[0074] Forsythia suspensa 6 parts;

[0075] One serving of Paris polyphylla;

[0076] Three parts of Dictamnus dasycarpus root bark;

[0077] Kochia scoparia fruit, 6 parts;

[0078] 8 portions of Salvia miltiorrhiza;

[0079] 3 parts safflower;

[0080] Two parts of Clematis armandii;

[0081] Centella asiatica 6 parts;

[0082] Two portions of Bletilla striata.

[0083] And it is prepared by the following method:

[0084] Step 1: Place the following herbs in a container: Phellodendron bark, Gardenia fruit, dried alum, honeysuckle, forsythia, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza, safflower, Clematis chinensis, Centella asiatica, and Bletilla striata. Add water to cover the herbs by 5cm. Soak at room temperature for 120 minutes. Heat to a boil and maintain boiling for 40 minutes. After solid-liquid separation, obtain the first decoction. Add water to the dregs to cover them by 2cm. Add water at 50℃ and heat to a boil. Maintain boiling for 40 minutes. After solid-liquid separation, obtain the second decoction and the dregs. Combine the first and second decoctions, mix them, and concentrate to obtain the medicinal liquid for later use.

[0085] Step 2: Mix the Chinese herbal residue obtained in Step 1 with PEG800 in a container and heat to 110℃. The mass ratio of the residue to PEG800 is 1:1. Then, use a high-speed shearing machine to shear the residue. First, shear at 1000 r / min for 80 min, and then increase the shearing speed to 5000 r / min for 120 min. Filter to obtain 400nm nano-Chinese herbal powder.

[0086] Step 3: Combine 70 parts of the traditional Chinese medicine liquid obtained in Step 1, 10 parts of the nano-sized traditional Chinese medicine powder obtained in Step 2, 4 parts of water, 10 parts of ethanol, 0.5 parts of Jema BP, 0.5 parts of silica with a particle size of 20nm and 5 parts of chitin. First, stir with a mixer at 400r / min for 80min, and then emulsify with a homogenizer at 12000r / min for 80min to obtain the original solution of traditional Chinese medicine nano-coated treatment.

[0087] Step 4: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 3. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. After stirring for 35 minutes, add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

[0088] Example 2

[0089] This embodiment provides a traditional Chinese medicine formula for promoting blood circulation and detoxification, and a method for preparing its nanogel, comprising the following components by weight:

[0090] Guan Huangbai 6 portions;

[0091] 12 portions of roasted gardenia;

[0092] 6 parts of calcined alum;

[0093] 12 portions of honeysuckle;

[0094] 12 portions of Forsythia suspensa;

[0095] Three portions of Paris polyphylla;

[0096] Five parts of Dictamnus dasycarpus root bark;

[0097] Kochia scoparia fruit, 12 parts;

[0098] 12 portions of Salvia miltiorrhiza;

[0099] Six parts of safflower;

[0100] 6 parts of Clematis armandii;

[0101] 10 parts Centella asiatica;

[0102] 8 parts of Bletilla striata.

[0103] And it is prepared by the following method:

[0104] Step 1: Place the following herbs in a container: Phellodendron bark, charred gardenia fruit, dried alum, honeysuckle, forsythia fruit, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza, safflower, Clematis chinensis, Centella asiatica, and Bletilla striata. Add water to cover the herbs by 2 cm. Soak at room temperature for 80 minutes. Heat to a boil and maintain boiling for 20 minutes. After solid-liquid separation, obtain the first decoction. Add water to the dregs to cover them by 0.5 cm. The water temperature should be 30℃. Heat to a boil and maintain boiling for 20 minutes. After solid-liquid separation, obtain the second decoction and the dregs. Combine the first and second decoctions, mix them, and concentrate to obtain the medicinal liquid for later use.

[0105] Step 2: Mix the Chinese herbal residue obtained in Step 1 with PEG800 in a container and heat to 90°C. The mass ratio of the herbal residue to PEG800 is 1:1. Then, use a high-speed shearing machine to shear the residue. First, shear at 600 r / min for 40 min, and then increase the shearing speed to 4500 r / min for 60 min. Filter to obtain 200 nm nano-sized Chinese herbal powder.

[0106] Step 3: Combine 70 parts of the traditional Chinese medicine liquid obtained in Step 1, 10 parts of the nano-sized traditional Chinese medicine powder obtained in Step 2, 4 parts of water, 10 parts of ethanol, 0.5 parts of Jema BP, 0.5 parts of silica with a particle size of 20nm and 5 parts of chitosan. First, stir with a mixer at 200r / min for 40min, and then emulsify with a homogenizer at 10000r / min for 60min to obtain the original solution of traditional Chinese medicine nano-coated treatment.

[0107] Step 4: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 3. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. After stirring for 40 minutes, add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

[0108] Example 3

[0109] This embodiment provides a traditional Chinese medicine formula for promoting blood circulation and detoxification, and a method for preparing its nanogel, comprising the following components by weight:

[0110] 10 portions of Coriander bark;

[0111] 10 portions of roasted gardenia;

[0112] 5 parts of calcined alum;

[0113] 8 portions of honeysuckle;

[0114] Forsythia suspensa 8 portions;

[0115] Two portions of Paris polyphylla;

[0116] Four parts of Dictamnus dasycarpus root bark;

[0117] Kochia scoparia fruit, 8 parts;

[0118] 10 portions of Salvia miltiorrhiza;

[0119] 5 parts safflower;

[0120] 4 parts of Clematis armandii;

[0121] 8 portions of Centella asiatica;

[0122] 5 parts of Bletilla striata.

[0123] And it is prepared by the following method:

[0124] Step 1: Place the following herbs in a container: Phellodendron bark, charred gardenia fruit, dried alum, honeysuckle, forsythia fruit, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza, safflower, Clematis chinensis, Centella asiatica, and Bletilla striata. Add water to cover the herbs by 3cm. Soak at room temperature for 100 minutes. Heat to a boil and maintain boiling for 30 minutes. After solid-liquid separation, obtain the first decoction. Add water to the dregs to cover them by 1cm. The water temperature should be 40℃. Heat to a boil and maintain boiling for 30 minutes. After solid-liquid separation, obtain the second decoction and the dregs. Combine the first and second decoctions, mix them, and concentrate to obtain the Chinese herbal medicine for later use.

[0125] Step 2: Mix the Chinese herbal residue obtained in Step 1 with PEG800 in a container and heat to 100℃. The mass ratio of the herbal residue to PEG800 is 1:1. Then, use a high-speed shearing machine to shear the residue. First, shear at 800 r / min for 60 min, and then increase the shearing speed to 4700 r / min for 90 min. Filter to obtain 300nm nano-Chinese herbal powder.

[0126] Step 3: Combine 70 parts of the traditional Chinese medicine liquid obtained in Step 1, 10 parts of the nano-sized traditional Chinese medicine powder obtained in Step 2, 4 parts of water, 10 parts of ethanol, 0.5 parts of Jema BP, 0.5 parts of silica with a particle size of 20nm and 5 parts of chitin. First, stir with a mixer at 300r / min for 60min, and then emulsify with a homogenizer at 11000r / min for 70min to obtain the original solution of traditional Chinese medicine nano-coated treatment.

[0127] Step 4: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 3. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. After stirring for 30 minutes, add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

[0128] Comparative Example 1

[0129] This comparative example provides a traditional Chinese medicine formula for promoting blood circulation and detoxification, and a method for preparing its nanogel, comprising the following components by weight:

[0130] 10 portions of Coriander bark;

[0131] 10 portions of roasted gardenia;

[0132] 5 parts of calcined alum;

[0133] 8 portions of honeysuckle;

[0134] Forsythia suspensa 8 portions;

[0135] Two portions of Paris polyphylla;

[0136] Four parts of Dictamnus dasycarpus root bark;

[0137] Kochia scoparia fruit, 8 parts;

[0138] 10 portions of Salvia miltiorrhiza;

[0139] 5 parts safflower;

[0140] 4 parts of Clematis armandii;

[0141] 8 portions of Centella asiatica;

[0142] 5 parts of Bletilla striata.

[0143] And it is prepared by the following method:

[0144] Step 1: Grind the following ingredients into powder: Phellodendron bark, Gardenia fruit, Alum, Honeysuckle, Forsythia fruit, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza, Safflower, Clematis chinensis, Centella asiatica, and Bletilla striata. Mix these powders with an equal mass of PEG800 in a container and heat to 100°C. Then, use a high-speed shearing machine to shear the powder. First, shear at 800 r / min for 60 min, and then increase the shearing speed to 4700 r / min for 90 min. Filter to obtain a 300 nm powder.

[0145] Step 2: Combine 80 parts of the powder obtained in Step 1, 4 parts of water, 10 parts of ethanol, 0.5 parts of Jema BP, 0.5 parts of silica with a particle size of 20nm and 5 parts of chitosan. First, stir with a mixer at 300r / min for 60min, and then emulsify with a homogenizer at 11000r / min for 70min to obtain the original solution of traditional Chinese medicine nano-coating treatment.

[0146] Step 3: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 2. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. After stirring for 30 minutes, add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

[0147] Application Example 1

[0148] The traditional Chinese medicine blood-activating and detoxifying nanogel prepared in Example 3 was used as drug 1, the traditional Chinese medicine blood-activating and detoxifying nanogel prepared in Comparative Example 1 was used as drug 2, the silver-containing dressing of Convide was used as drug 3, and the physiological saline dressing was used as drug 4.

[0149] Before use, thoroughly clean the wound and surrounding skin with sterile saline or povidone-iodine to ensure a sterile environment. After thorough debridement, apply medication 1-4 to treat the wound, changing it every 48 hours. If there is excessive exudation, the frequency can be increased to once daily. The treatment period is 4 weeks, or until the wound is completely healed.

[0150] For patients aged 18-75 who meet the diagnostic criteria for chronic wounds (wound duration > 4 weeks) and have a wound area of ​​2-10 cm², the criteria are considered. 2 And accompanied by clear evidence of bacterial infection (colony count >10). 5 Patients with CFU / g tissue were included in the treatment program, while patients with severe organ dysfunction, immunosuppression, known allergy to gel components, pregnancy, lactation, or inability to cooperate with follow-up were excluded. From June 2023 to May 2025, a total of 248 patients with chronic wound infection meeting the above inclusion and exclusion criteria were collected and randomly divided into 4 groups, with 62 patients in each group, and treated with drugs 1-4 respectively.

[0151] There were no significant differences among the four groups of patients in terms of age, disease duration, and chronic wound classification and grading. A double-blind design was used for both subjects and efficacy assessors, with only nursing staff aware of the group assignments. The gel and control dressings used identical packaging to ensure the effectiveness of the blinding method. The efficacy assessment indicators are as follows:

[0152] 1. Wound healing rate

[0153] The area change was calculated using digital planar measurement. Efficacy criteria: a closure rate ≥90% after 4 weeks was considered significantly effective; 70-89% was considered effective; <70% was considered ineffective.

[0154] 2. Complete healing time

[0155] The number of days required for complete epithelialization of the wound.

[0156] 3. Safety evaluation

[0157] ① Local adverse reactions: pain level (VAS score, 0-10), incidence of allergic reactions such as contact dermatitis and itching, and recording and observation of skin irritation symptoms around the wound.

[0158] ②Systemic safety: Liver and kidney function monitoring, complete blood count and high-sensitivity C-reactive protein monitoring.

[0159] The experimental results are shown in the table below:

[0160]

[0161] Results analysis: The above experimental data show that drug 1 has a more significant therapeutic effect than drugs 2-4, achieving the expected purpose of this invention.

[0162] The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which this invention pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of the invention or exceeding the scope defined by the appended claims.

Claims

1. A traditional Chinese medicine formula for promoting blood circulation and detoxifying, comprising principal, assistant, adjuvant, and guiding herbs, characterized in that: The principal herbs include Phellodendron bark, Gardenia fruit, and alum; the assistant herbs include honeysuckle, forsythia, Paris rhizome, Dictamnus root bark, and Kochia fruit; the adjuvant herbs include Salvia miltiorrhiza, safflower, Clematis chinensis, and Centella asiatica; and the guiding herb includes Bletilla striata.

2. The traditional Chinese medicine formula for promoting blood circulation and detoxifying as described in claim 1, characterized in that: The traditional Chinese medicine formula includes the following components in parts by weight: 6-12 parts of Cortex Phellodendri; 6-12 portions of roasted gardenia fruit; 3-6 parts of calcined alum; Honeysuckle 6-12 parts; Forsythia suspensa 6-12 portions; 1-3 portions of Paris polyphylla; 3-5 parts of Dictamnus dasycarpus root bark; Kochia scoparia fruit, 6-12 parts; 8-12 parts of Salvia miltiorrhiza; 3-6 parts safflower; 2-6 parts of Clematis armandii; Centella asiatica 6-10 parts; 2-8 parts of Bletilla striata.

3. The traditional Chinese medicine formula for promoting blood circulation and detoxifying as described in claim 2, characterized in that: The traditional Chinese medicine formula includes the following components in parts by weight: 10 portions of Coriander bark; 10 portions of roasted gardenia; 5 parts of calcined alum; 8 portions of honeysuckle; Forsythia suspensa 8 portions; Two portions of Paris polyphylla; Four parts of Dictamnus dasycarpus root bark; Kochia scoparia fruit, 8 parts; 10 portions of Salvia miltiorrhiza; 5 parts safflower; 4 parts of Clematis armandii; 8 portions of Centella asiatica; 5 parts of Bletilla striata.

4. The preparation method of the blood-activating and detoxifying traditional Chinese medicine formulation nanogel as described in any one of claims 1-3, characterized in that, Includes the following steps: Step 1: Place the following herbs in a container: Phellodendron bark, Gardenia fruit, dried alum, honeysuckle, forsythia, Paris rhizome, Dictamnus root bark, Kochia fruit, Salvia miltiorrhiza, safflower, Clematis chinensis, Centella asiatica, and Bletilla striata. Add water to cover the herbs and soak at room temperature for 80-120 minutes. Heat to a boil and maintain boiling for 20-40 minutes. After solid-liquid separation, obtain the first decoction. Add water to the dregs and heat to a boil, maintaining boiling for 20-40 minutes. After solid-liquid separation, obtain the second decoction and the dregs. Combine the first and second decoctions, mix them, and concentrate to obtain the medicinal liquid for later use. Step 2: Mix the Chinese herbal residue obtained in Step 1 with PEG800 in a container and heat to 90-110℃. Then, use a high-speed shearing machine to shear and filter to obtain 200-400nm nano-sized Chinese herbal powder. Step 3: Combine the herbal liquid obtained in Step 1, the nano-herbal powder obtained in Step 2, water, ethanol, Jema BP, silica and chitosan. First, stir with a mixer at 200-400 rpm for 40-80 minutes, and then emulsify with a homogenizer at 10000-12000 rpm for 60-80 minutes to obtain the herbal nano-coated stock solution. Step 4: Add carbomer powder to the herbal nano-encapsulation treatment stock solution obtained in Step 3. The mass of carbomer powder is 1.5% of the mass of the herbal nano-encapsulation treatment stock solution. Stir for more than 30 minutes, then add triethanolamine and stir to adjust the gel viscosity. Finally, a herbal blood-activating and detoxifying nano gel with a gel viscosity of 20,000 cp is formed.

5. The method for preparing nanogels as described in claim 4, characterized in that: In step one, water is added to cover the medicinal materials placed in the container by 2-5 cm.

6. The method for preparing nanogels as described in claim 4, characterized in that: In step one, water is added to the dregs to cover them by 0.5-2cm, and the temperature of the water is 30-50℃.

7. The method for preparing nanogels as described in claim 4, characterized in that: The mass ratio of the Chinese herbal medicine residue to PEG800 in step two is 1:

1.

8. The method for preparing nanogels as described in claim 4, characterized in that: The shearing step in step two is as follows: first, shear at a speed of 600-1000 r / min for 40-80 min, and then increase the shearing speed to 4500-5000 r / min for 60-120 min.

9. The method for preparing nanogels as described in claim 4, characterized in that: In step three, 70 parts by weight of traditional Chinese medicine liquid, 10 parts by weight of nano-traditional Chinese medicine powder, 4 parts by weight of water, 10 parts by weight of ethanol, 0.5 parts by weight of Jema BP, 0.5 parts by weight of silicon dioxide and 5 parts by weight of chitin are added to form a compound.

10. The method for preparing the nanogel according to claim 9, characterized in that: The particle size of the silicon dioxide in step three is 20 nm.

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