Nanometer trapping agent for bactrocera dorsalis as well as preparation method and application of nanometer trapping agent

Abstract

The invention belongs to the technical field of trapping agents, and particularly relates to a bactrocera dorsalis nano trapping agent and a preparation method and application thereof. The bactrocera dorsalis nanometer trapping agent is prepared by compounding methyleugenol and a nanometer carrier HLDP according to the mass ratio of 1: (0.5-6). The bactrocera dorsalis nano trapping agent provided by the invention is excellent in trapping effect, reduces the dosage of methyleugenol to a certain extent, and is beneficial to efficient prevention and control and green prevention and control of bactrocera dorsalis.

Description

Technical Field

[0001] This invention belongs to the field of attractant technology, specifically relating to a nano-attractant for the oriental fruit fly, its preparation method, and its application. Background Technology

[0002] The oriental fruit fly, its Latin name is Bactrocera dorsalis Hendel. The oriental fruit fly is an important invasive pest belonging to the family Orientalis in the order Diptera. It has a high reproductive rate and a wide host range, damaging nearly 250 kinds of fruits and vegetables, including pomegranates, mangoes, citrus fruits, peaches, and pears, and can have multiple generations per year. The oriental fruit fly mainly damages fruit by having adult females lay eggs inside the fruit and the larvae feed inside the fruit, causing fruit drop and rot, seriously threatening fruit yield and quality.

[0003] Currently, the main direct control method for the oriental fruit fly (Bactrocera dorsalis) is to use methyl eugenol, a specific attractant for male Bactrocera dorsalis, to trap males and reduce the mating rate and egg production of females, thereby achieving the goal of controlling the fly. However, males can mate multiple times, up to 34 times in their lifetime. In field application, large amounts of methyl eugenol are used, and it only traps a portion of the males, failing to effectively reduce the mating rate, thus limiting the actual control effect. Therefore, achieving reduced and efficient utilization of methyl eugenol and developing a compound attractant that can further enhance its trapping effect is a key issue that urgently needs attention. Summary of the Invention

[0004] To address the limitations of existing technologies that require large amounts of methyl eugenol, only attracting a portion of male flies and failing to effectively reduce mating rates, thus resulting in limited practical control effects, this invention utilizes a nanocarrier HLDP loaded with methyl eugenol to create a nano-attractant for the oriental fruit fly (Bactrocera dorsalis). This invention also provides the oriental fruit fly nano-attractant, its preparation method, and its application. To achieve the above objectives, this invention adopts the following technical solution: This invention provides an oriental fruit fly nano-attractant, which is obtained by compounding methyl eugenol and the nanocarrier HLDP at a mass ratio of 1:0.5~6.

[0005] The structural formula of the HLDP nanocarrier is as follows: .

[0006] Preferably, the oriental fruit fly nano-attractant is obtained by compounding eugenol and nanocarrier HLDP at a mass ratio of 1:0.65~5.

[0007] Preferably, the oriental fruit fly nano-attractant is obtained by compounding methyl eugenol and nanocarrier HLDP at a mass ratio of 1:0.85~3.

[0008] The application provides a B. dorsalis nanometer trapping agent which is formed by compounding methyl eugenol and a nanometer carrier HLDP at a mass ratio of 1:0.5-6. The B. dorsalis nanometer trapping agent has low particle size and low surface tension, and has strong volatilization capacity. When used in the field, the number of male insects trapped can be significantly increased, and the mating rate of female and male insects can be effectively reduced. Moreover, the B. dorsalis nanometer trapping agent improves the trapping effect of methyl eugenol, reduces the use dose of methyl eugenol to a certain extent, and is helpful for efficient prevention and control of B. dorsalis.

[0009] The application further provides a preparation method of the B. dorsalis nanometer trapping agent. The methyl eugenol and the nanometer carrier HLDP are taken at a mass ratio, respectively.

[0010] The methyl eugenol and the nanometer carrier HLDP are mixed uniformly, and are incubated at room temperature for 5 min-30 min, so that the B. dorsalis nanometer trapping agent is obtained.

[0011] The application further provides application of the B. dorsalis nanometer trapping agent in trapping B. dorsalis.

[0012] Preferably, in use, the B. dorsalis nanometer trapping agent is prepared into a nanometer trapping agent solution, and is placed in a trapping bottle or a trap.

[0013] Preferably, the nanometer trapping agent solution is directly added into the trapping bottle, and the trapping bottle is placed in the field for use; or a rubber plug is soaked in the nanometer trapping agent solution, the soaked rubber plug is fixed in the trap, and the trap is placed in the field for use.

[0014] The concentration of methyl eugenol in the nanometer trapping agent solution is 14.2 g / 100 mL-14.4 g / 100 mL, and the concentration of the nanometer carrier HLDP in the nanometer trapping agent solution is 7.2 g / 100 mL-85.8 g / 100 mL.

[0015] Preferably, the trapping bottle or the trap is hung at a position 1.4 m-1.6 m above the crops planted in the field.

[0016] Preferably, the nanometer trapping agent solution is first added to a lure core cotton swab, and then the lure core cotton swab is fixed in the trapping bottle.

[0017] Preferably, 1 mL-2 mL of the nanometer trapping agent solution is added to each lure core cotton swab.

[0018] Compared with the prior art, the application has the following beneficial effects: The application provides a Bactrocera dorsalis nanometer trapping agent which is formed by compounding methyl eugenol and a nanometer carrier HLDP at a mass ratio of 1:0.5-6. The Bactrocera dorsalis nanometer trapping agent has low particle size and low surface tension, and has strong volatilization capacity. When used in the field, the number of male insects trapped can be significantly increased, and the mating rate of female and male insects can be effectively reduced. Moreover, the Bactrocera dorsalis nanometer trapping agent improves the trapping effect of methyl eugenol, reduces the use dose of methyl eugenol to a certain extent, and is helpful to efficient prevention and control of Bactrocera dorsalis. Therefore, the Bactrocera dorsalis nanometer trapping agent can solve the problems in the prior art that the use dose of methyl eugenol is large, only part of male insects can be trapped, the mating rate cannot be effectively reduced, and thus the actual prevention and control effect is limited. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The structural formula of the nanometer carrier HLDP in the application.

[0020] Figure 2 The structural formula of methyl eugenol in the application.

[0021] Figure 3 The particle size and morphology of the Bactrocera dorsalis nanometer trapping agent in the application; wherein, Figure 3 The A graph in the figure is the particle size of the Bactrocera dorsalis nanometer trapping agent; Figure 3 The B graph in the figure is the morphology of the Bactrocera dorsalis nanometer trapping agent.

[0022] Figure 4 The surface tension of the Bactrocera dorsalis nanometer trapping agent in the application.

[0023] Figure 5 The volatilization rate of the Bactrocera dorsalis nanometer trapping agent in the application; wherein, Figure 5 The A graph in the figure is the volatilization amount of the Bactrocera dorsalis nanometer trapping agent at different times; Figure 5 The B graph in the figure is the cumulative volatilization dose of the Bactrocera dorsalis nanometer trapping agent at different times.

[0024] Figure 6 The trapping effect evaluation of the Bactrocera dorsalis nanometer trapping agent in the application in a glass Y-type tube olfactometer.

[0025] Figure 7 The trapping effect evaluation of the Bactrocera dorsalis nanometer trapping agent in the application in a greenhouse; wherein, Figure 7 The A graph in the figure is the average trapping amount of the Bactrocera dorsalis nanometer trapping agent; Figure 7 The B graph in the figure is the total trapping amount of the Bactrocera dorsalis nanometer trapping agent.

[0026] Figure 8 The trapping effect evaluation of the Bactrocera dorsalis nanometer trapping agent in the application in the field; wherein, Figure 8A chart in FIG. is the distribution of the trapping amount of the Bactrocera dorsalis nanometer attractant at different times; Figure 8 B chart in FIG. is the total trapping amount of the Bactrocera dorsalis nanometer attractant at different times. DETAILED DESCRIPTION

[0027] The application will be described in detail below with reference to the drawings and specific examples, but should not be understood as limiting the application. If not specially stated, the technical means used in the following examples are the conventional means familiar to those skilled in the art, and the materials, reagents, etc. used in the following examples, if not specially stated, can be obtained from commercial channels.

[0028] Reagents and materials and reagents are as follows: Methyl eugenol is purchased from Jiangxi Hairei Natural Plant Co., Ltd., and the item number is methyl DXF. Among them, the English abbreviation of methyl eugenol is ME. The structural formula of methyl eugenol is as shown in Figure 2 .

[0029] The nanocarrier HLDP is a nanomaterial named HLDP disclosed in the Chinese patent with the patent number CN 118791684 A, entitled Preparation and application of a nanomaterial for preventing and treating mango anthracnose and its complex.

[0030] The preparation method of the nanocarrier HLDP is prepared by referring to the preparation method in the above-mentioned patent. The structural formula of the nanocarrier HLDP is as shown in Figure 1 .

[0031] Example 1 A preparation method of a Bactrocera dorsalis nanometer attractant, comprising the following steps: 1g of methyl eugenol and 1g of nanocarrier HLDP are weighed respectively, the methyl eugenol and the nanocarrier HLDP are added together into a test tube containing 7mL of deionized water, and then vortexed until mixed uniformly, and then incubated at room temperature for 15min to obtain the Bactrocera dorsalis nanometer attractant.

[0032] Example 2 A preparation method of a Bactrocera dorsalis nanometer attractant, comprising the following steps: 1g of methyl eugenol and 0.5g of nanocarrier HLDP are weighed respectively, the methyl eugenol and the nanocarrier HLDP are added together into a test tube containing 7mL of deionized water, and then vortexed until mixed uniformly, and then incubated at room temperature for 15min to obtain the Bactrocera dorsalis nanometer attractant.

[0033] Example 3 A preparation method of a Bactrocera dorsalis nanometer attractant, comprising the following steps: Take 1g methyl eugenol and 6g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0034] Example 4 A preparation method of a B. dorsalis nano-attractant, comprising the following steps: Take 1g methyl eugenol and 3g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0035] Example 5 A preparation method of a B. dorsalis nano-attractant, comprising the following steps: Take 1g methyl eugenol and 0.65g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0036] Example 6 A preparation method of a B. dorsalis nano-attractant, comprising the following steps: Take 1g methyl eugenol and 5g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0037] Example 7 A preparation method of a B. dorsalis nano-attractant, comprising the following steps: Take 1g methyl eugenol and 2.8g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0038] Example 8 A preparation method of a B. dorsalis nano-attractant, comprising the following steps: Take 1g methyl eugenol and 0.85g nano-carrier HLDP respectively, add the attractant methyl eugenol and nano-carrier HLDP into a test tube containing 7mL deionized water, then vortex until mixed evenly, and then incubate at room temperature for 15min to prepare the B. dorsalis nano-attractant.

[0039] Example 9 A preparation method of the B. dorsalis nanolurent, comprising the following steps: 1g of methyl eugenol and 3.5g of the nanocarrier HLDP were weighed respectively, and the nanolurent methyl eugenol and the nanocarrier HLDP were added into a test tube containing 7mL of deionized water, and then vortexed until mixed uniformly, and then incubated at room temperature for 15min to prepare the B. dorsalis nanolurent.

[0040] Example 10 A preparation method of the B. dorsalis nanolurent, comprising the following steps: 1g of methyl eugenol and 1.8g of the nanocarrier HLDP were weighed respectively, and the nanolurent methyl eugenol and the nanocarrier HLDP were added into a test tube containing 7mL of deionized water, and then vortexed until mixed uniformly, and then incubated at room temperature for 15min to prepare the B. dorsalis nanolurent.

[0041] In order to illustrate the effect of the B. dorsalis nanolurent provided by the application, the B. dorsalis nanolurent prepared in Example 1 was selected as an example, and the following research was conducted: 1. Morphology characterization The particle size and morphology of the B. dorsalis nanolurent prepared in Example 1 were determined by using a nanoparticle size analyzer and a scanning electron microscope. The specific steps are as follows:

[0042] 3mL of the B. dorsalis nanolurent was taken in a quartz glass dish and placed in a nanoparticle size analyzer for particle size detection. 10μL of the B. dorsalis nanolurent was taken in a silicon wafer with a pipette, dried at room temperature, and then gold was sprayed, and then the morphology was observed under a scanning electron microscope.

[0043] Scanning electron microscope: Japan Hitachi Company, model S4800.

[0044] Nanoparticle size analyzer: British Malvern Panalytical Company, model Zetasizer Nano ZS.

[0045] Structural characteristics of the nanocarrier HLDP: a linear block polymer, the structural formula is as shown in Figure 1 .

[0046] Structural characteristics of methyl eugenol: molecular formula is C 11 H 14 O2, belonging to phenylpropane compounds, the structural formula is as shown in Figure 2 .

[0047] The average particle size of the B. dorsalis nanolurent prepared in Example 1 was about 18nm measured by using a nanoparticle size analyzer, and the morphology observed under a scanning electron microscope showed that it was a spherical particle, as shown inFigure 3 As shown.

[0048] 2. Determination of the volatility characteristics of nano-attractants for the citrus fruit fly Take the oriental fruit fly nano-attractant prepared in Example 1, and at the same time prepare nano-carrier HLDP solution and methyl eugenol solution with the same concentration as the oriental fruit fly nano-attractant prepared in Example 1. Use water as a blank control. Use a surface tension meter to test the surface tension of the above 4 samples respectively, and repeat the test 3 times for each sample.

[0049] The preparation method of the HLDP nanocarrier solution is as follows: take 1g of HLDP nanocarrier and add it to a test tube containing 8mL of deionized water, then vortex until it is uniformly dissolved to obtain the HLDP nanocarrier solution.

[0050] The method for preparing methyl eugenol solution is as follows: Take 1g of methyl eugenol and add it to a test tube containing 7mL of deionized water, then vortex until it is evenly dispersed to obtain methyl eugenol solution.

[0051] Surface tension measuring instrument: Dataphysics GmbH, Germany, model DCAT 21.

[0052] The surface tension of the oriental fruit fly nano-attractant prepared in Example 1 was measured as follows: Figure 4 As shown.

[0053] Depend on Figure 4 It can be found that the average surface tension of the oriental fruit fly nano-attractant prepared in Example 1 is 36.82 mN / m, while the surface tension of the methyl eugenol solution is 44.43 mN / m. The surface tension of the nano-attractant is significantly lower than that of the methyl eugenol solution.

[0054] The above experimental results show that the addition of the nanocarrier HLDP significantly reduced the surface tension of the attracting active factor methyleugenol in the nano-attractant, thus the oriental fruit fly nano-attractant prepared in Example 1 has better physicochemical properties.

[0055] 3. Volatility characteristics of the oriental fruit fly nano-attractant Two mL of the oriental fruit fly nano-attractant prepared in Example 1 and a methyl eugenol solution of the same concentration were adsorbed onto cotton swabs. The cotton swabs with the adsorbed samples were then placed in a volatile collection device. This experiment consisted of two treatments, with four replicates for each treatment. Volatile matter was collected every 48 hours. The gas sampler parameters were set to 1 L / min, and sampling was performed continuously for 4 hours each time, collecting data at three time points.

[0056] The preparation method for the methyl eugenol solution is the same as above.

[0057] After the collection, 5 mL of chromatographic methanol was used to elute the volatiles, followed by filtration with a 0.22 μm filter membrane for use. A standard solution of methyl eugenol was prepared using chromatographic methanol, with concentrations of 50 μg / mL, 100 μg / mL, 300 μg / mL, 500 μg / mL, 700 μg / mL, and 1000 μg / mL. An ultra-high performance liquid chromatograph was used to establish a standard curve of methyl eugenol and to detect the content of methyl eugenol in the volatiles.

[0058] The ultra-high performance liquid chromatograph was from Agilent Technologies, with a model number of Agilent 1290.

[0059] The elution conditions of the high performance liquid chromatograph were as follows: the mobile phase was acetonitrile: water = 92:8, V / V; the flow rate was 1 mL / min; the injection volume was 4 μL; the detector was an ultraviolet detector; and the detection wavelength was 290 nm.

[0060] The results of the volatilization characteristics of the B. dorsalis nano-lure prepared in Example 1 are shown in Table 1. Figure 5

[0061] The volatilization of methyl eugenol was quantified at three time points, and it was found that the nano-lure volatilized the most methyl eugenol in the same time, with the fastest volatilization rate, and the cumulative volatilization dose was also much higher than that of the methyl eugenol solution at the same concentration.

[0062] The above experimental results show that the nano-lure has excellent volatilization performance, significantly improving the volatilization rate of methyl eugenol.

[0063] 4. Evaluation of the trapping effect of the B. dorsalis nano-lure The trapping effect of the nano-lure was evaluated in a glass Y-tube olfactometer, a greenhouse, and a field. The specific methods were as follows:

[0064] (1) Glass Y-tube olfactometer ​The glass Y-tube olfactometer is mainly composed of a rubber hose connected in turn with an air sampling instrument, an activated carbon bottle, a smell source bottle and a Y-tube, wherein the Y-tube has an arm length of 15 cm and an inner diameter of 1 cm. The Y-tube olfactometer is used to test the selection effect of B. dorsalis between four groups of substances. The four groups of substances are water and nano-carrier HLDP solution, water and methyl eugenol solution, water and nano-trapping agent, and methyl eugenol solution and nano-trapping agent. Taking the selection effect of B. dorsalis between water and nano-carrier HLDP solution as an example, 5 mL of water and 5 mL of nano-carrier HLDP solution are respectively added into the smell source bottles connected with the two arms of the Y-tube, and one male B. dorsalis is placed at the end of the straight arm of the Y-tube. In the experiment, the flow rate of the air sampling instrument is kept at 1 L / min, and each B. dorsalis is observed for 5 min. If the position of the B. dorsalis exceeds 1 / 2 of the arm and is maintained for 30 s, it is considered to have a selection. The experiment tests 25 immature and mature B. dorsalis respectively.

[0065] It should be noted that before the test insects are placed, the above device needs to be ventilated for 17.5±2.5 min to ensure that the substances in the smell source bottles fill the connected Y-tube arms. In the experiment, the Y-tube needs to be replaced and the smell source bottle positions need to be exchanged every 5 test insects to avoid experimental interference and errors caused by position effects. The remaining three groups of substances are tested in the same way.

[0066] Among them, the immature B. dorsalis refers to B. dorsalis that has been hatched for 5 days. The mature B. dorsalis refers to B. dorsalis that has been hatched for 15 days.

[0067] The above B. dorsalis is from a sensitive population that has been bred in the laboratory of China Agricultural University for more than 30 generations.

[0068] Water is used as a solvent control, and deionized water is used; nano-carrier HLDP is used as a nano-carrier HLDP solution; methyl eugenol is used as a methyl eugenol solution; and the nano-trapping agent experiment uses the B. dorsalis nano-trapping agent prepared in Example 1, and the concentration is 14.3 g / 100 mL.

[0069] The preparation method of the nano-carrier HLDP solution and the methyl eugenol solution is as above.

[0070] The results of the experiment in the glass Y-tube olfactometer are shown in Table 1. Figure 6

[0071] Figure 6 ​The results showed that immature insects exhibited virtually no response to methyleugenol, showing no significant selective effect between methyleugenol solution and the nano-attractant. However, once the insects reached sexual maturity, they showed a strong selectivity for methyleugenol, exhibiting a significant selective effect towards the nano-attractant over the methyleugenol solution. Therefore, the nano-attractant demonstrated excellent attraction to the oriental fruit fly and could enhance the attraction activity of methyleugenol.

[0072] (2) Greenhouse The greenhouse used in the greenhouse experiment had the following dimensions: length × width × height = 16.3m × 3.25m × 7m. The specific method is as follows:

[0073] Approximately 900 sexually mature male oriental fruit flies were released one day in advance to ensure even distribution of the population. Two treatment groups were selected: a methyl eugenol solution and the oriental fruit fly nano-attractant prepared in Example 1. 1 mL of each treatment solution was applied to a cotton swab to form a lure, which was then assembled into a trap to form a trap bottle. Each trap bottle was considered a replicate, and 15 replicates were set up for each treatment. All trap bottles were randomly suspended in the greenhouse, and the number of insects in each trap bottle was counted one day later.

[0074] The results of the greenhouse experiment are as follows Figure 7 As shown. Figure 7 The results showed that the nano-attractant had a significantly higher trapping ability for oriental fruit flies than methyl eugenol, and in terms of the total amount of attractants, it also far exceeded that of methyl eugenol.

[0075] (3) Daejeon A field trapping experiment was conducted in a pomegranate orchard, with two treatment groups: methyl eugenol and nano-attractants. 1 mL of each treatment solution was transferred to a cotton swab to form a lure, which was then assembled into a trap bottle. Each trap bottle was considered a replicate, and 60 replicates were set up for each treatment. One trap bottle was hung on each pomegranate tree, alternating between the two treatments, at a height of approximately 1.5 m. The number of insects trapped in each trap bottle was assessed weekly for six consecutive weeks.

[0076] The methyl eugenol treatment group used a methyl eugenol solution; the nano-attractant treatment group used the oriental fruit fly nano-attractant prepared in Example 1, wherein the concentration of methyl eugenol was 14.3 g / 100 mL.

[0077] The results of the field trapping experiment are as follows Figure 8 As shown in the figure, the nano-attractant exhibited excellent trapping ability at all six survey time points, and the total amount trapped was far higher than that of methyl eugenol. These results are consistent with those obtained using a glass Y-tube olfactometer and in a greenhouse. Furthermore, field trials revealed that the nano-attractant's field residual effect lasted approximately 35 days, comparable to that of commercially available attractants.

[0078] From the above results, the B. dorsalis nano trapping agent has excellent physical and chemical properties and trapping effect.

[0079] The application successfully prepares a nano trapping agent capable of efficiently trapping male B. dorsalis by using the nano carrier HLDP, significantly improves the field trapping capacity of methyl eugenol on B. dorsalis, and to a certain extent, reduces the use amount of methyl eugenol and the environmental risk of agricultural products. In summary, the present study is conducive to the efficient prevention and control and safe prevention and control of B. dorsalis.

[0080] Although the preferred embodiments of the present application have been described, those skilled in the art can make further changes and modifications to these embodiments once they know the basic inventive concept, and all changes and modifications falling within the scope of the present application.

Claims

1. A B. dorsalis nanotrap agent, characterized in that, The B. minores nano-lure is compounded by eugenol and nano-carrier HLDP according to a mass ratio of 1:0.5-6. The nano-carrier HLDP has the following structural formula: 。 2. The B. dorsalis nano-lure according to claim 1, characterized in that, The B. minores nano-lure is compounded by eugenol and nano-carrier HLDP according to a mass ratio of 1:0.65-5.

3. The B. dorsalis nano-lure according to claim 2, characterized in that, The B. minores nano-lure is compounded by eugenol and nano-carrier HLDP according to a mass ratio of 1:0.85-3.

4. The method of claim 1, wherein the method of preparing a B. dorsalis nano-lure is characterized by, The method comprises the following steps: The eugenol and the nano-carrier HLDP are taken according to a mass ratio of 1:0.85-3 respectively; The eugenol and the nano-carrier HLDP are mixed uniformly, and are incubated at room temperature for 5-30 minutes to obtain the B. minores nano-lure.

5. The B. minores nano-lure of claim 1 is applied to trapping B. minores.

6. Use according to claim 5, characterized in that, In use, the B. minores nano-lure is prepared into a nano-lure solution, and is placed in a lure bottle or a trap.

7. Use according to claim 6, characterized in that, The nano-lure solution is directly added into the lure bottle, and the lure bottle is placed in a field for use; or a rubber plug is soaked in the nano-lure solution, and the soaked rubber plug is fixed in the trap, and the trap is placed in a field for use. The concentration of eugenol in the nano-lure solution is 14.2-14.4 g / 100 mL, and the concentration of nano-carrier HLDP in the nano-lure solution is 7.2-85.8 g / 100 mL.

8. Use according to claim 7, characterized in that, The lure bottle or the trap is hung at a height of 1.4-1.6 m above crops planted in a field.

9. Use according to claim 7, characterized in that, The nano-lure solution is first added to a lure core cotton swab, and then the lure core cotton swab is fixed in the lure bottle.

10. Use according to claim 9, characterized in that, 1-2 mL of the nano-lure solution is added to each lure core cotton swab.

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