Liquid transfer device, reaction device, analysis device, and liquid transfer method

Abstract

The invention discloses a liquid transfer device, a reaction device, an analysis device and a liquid transfer method. The liquid transfer device is used for transferring the liquid carried by the first base material to the sample carrier. The liquid transfer device comprises a fixing part and a first supporting part. The fixing part comprises a fixing structure which is used for fixing the sample carrier. The first supporting part and the fixing structure are oppositely arranged in a spaced mode in the first direction. The first supporting part is provided with a first supporting surface. The first supporting surface is configured to at least partially face the reaction surface of the sample carrier after the sample carrier is fixed by the fixing structure. Wherein the first supporting surface is used for supporting the first base material, so that liquid carried by the first base material supported by the first supporting surface is transferred to the reaction surface of the sample carrier fixed by the fixing structure. According to the liquid transfer device, the reaction device, the analysis device and the liquid transfer method, shaking of the first base material can be effectively inhibited, the first base material is prevented from scraping the sample carrier, and the liquid transfer stability is improved.

Description

Liquid transfer device, reaction device, analysis device and liquid transfer method Technical Field

[0001] The present invention relates to the technical field of biochemical reactions, and in particular to a liquid transfer device, a reaction device, an analysis device and a liquid transfer method. Background Art

[0002] Through gene sequencing, the sequence of target gene fragments can be obtained to facilitate further molecular biology research and gene modification. It can be used to study various diseases and gene expression, and is of great significance in many fields such as biology, medicine and environmental science.

[0003] Currently, mainstream high-throughput sequencing technology is based on the principle of sequencing by synthesis, where DNA nanospheres are formed within the prepared library to amplify fluorescent signals. These nanospheres are then affixed to an arrayed sequencing chip according to a specific pattern and spacing. Through each round of reaction with a specific enzyme and fluorescent probe, different bases emit fluorescent signals of varying wavelengths. These signals are captured by an on-board sequencing imaging system, and the images are reconstructed and identified to sequentially determine the base sequence for each round.

[0004] Existing high-throughput sequencing technologies widely utilize flow cells to perform the sequencing process. These cells perform multiple rounds of imaging on a planar medium within the flow cell to identify bases. However, the biochemical reactions between imaging steps require precise temperature and fluid control, creating demanding environmental requirements. Furthermore, each cycle completely replaces the previous reagent, requiring a large amount of the next reagent to be flushed before the reaction can proceed, resulting in high reagent consumption. The sequencing process requires multiple reagents to undergo up to hundreds of "reaction-photography" cycles, resulting in low sequencing efficiency and high production costs.

[0005] In related technologies, to reduce costs and increase throughput, liquid transfer devices are used to accomplish liquid transfer. Specifically, a thin layer of reagent is coated on the surface of a film substrate, which is then driven toward a sample carrier. The moving film substrate passes through the sample carrier and transfers the reagent layer it carries to the sample carrier, thereby achieving liquid transfer. However, because the film substrate is made of a flexible material, it can vibrate during the process of moving the reagent layer and the film substrate toward the sample carrier, causing it to easily rub against the sample carrier, affecting the stability of the liquid transfer.

[0006] Summary of the Invention

[0007] The main purpose of the present invention is to provide a liquid transfer device, a reaction device, an analysis device and a liquid transfer method to effectively suppress the vibration of the first substrate, prevent the first substrate from scratching the sample carrier, and improve the stability of liquid transfer.

[0008] A first embodiment of the present invention provides a liquid transfer device for transferring liquid carried by a first substrate to a sample carrier, the liquid transfer device comprising:

[0009] a fixing portion, comprising a fixing structure, wherein the fixing structure is used to fix the sample carrier; and

[0010] a first supporting portion, arranged opposite to the fixing structure along a first direction and spaced apart from each other, the first supporting portion having a first supporting surface, the first supporting surface being configured to at least partially face the reaction surface of the sample carrier when the fixing structure fixes the sample carrier;

[0011] The first supporting surface is used to support the first substrate, so that the liquid carried by the first substrate supported by the first supporting surface is transferred to the reaction surface of the sample carrier fixed by the fixing structure.

[0012] In some embodiments, the liquid transfer device also includes a first adjustment mechanism, which is connected to the fixed structure and can adjust the relative position of the fixed structure and the first support surface along the first direction, so that the sample carrier fixed to the fixed structure has at least two positioning positions with different distances from the first support surface.

[0013] In some embodiments, the liquid transfer device also includes a second adjustment mechanism, which is connected to the fixed structure and can adjust the relative position of the fixed structure and the first support surface along a direction perpendicular to the first direction, and the fixed structure can be adjusted by the second adjustment mechanism so that the sample carrier fixed to the fixed structure and the first support surface do not overlap along the first direction.

[0014] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0015] The second adjustment mechanism is configured to adjust the relative position of the fixed structure and the first support surface along the second direction or the opposite direction of the second direction, and the fixed structure can be adjusted by the second adjustment mechanism so that the sample carrier fixed to the fixed structure does not overlap with the first support surface along the first direction.

[0016] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0017] The first supporting surface is an arc surface, the axis of the arc surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction.

[0018] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0019] The first supporting surface is a cylinder, and a central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction.

[0020] In some embodiments, the cylindrical surface is a cylindrical surface, and the first supporting portion is configured to be driven by the first substrate to rotate about a central axis of the cylindrical surface as a rotation axis.

[0021] In some embodiments, the first supporting surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to the third direction, and the third direction is perpendicular to the first direction. The liquid transfer device further includes a first driving mechanism, the first driving mechanism being configured to drive the first supporting portion to rotate about the central axis of the cylindrical surface, thereby driving the first substrate to move;

[0022] At the liquid exchange position between the first substrate and the sample carrier, the first supporting portion is configured to drive the first substrate to move along a second direction perpendicular to the first direction and the third direction.

[0023] In some embodiments, the first supporting surface has a boundary line arranged parallel to the third direction, and along the first direction, the boundary line is located at an end of the first supporting surface facing the fixed structure;

[0024] The liquid transfer device also includes a second driving mechanism, which is connected to the fixed structure and is configured to drive the fixed structure to reciprocate along the second direction or the reverse direction of the second direction, so that after the fixed structure fixes the sample carrier, when observed along the first direction, the boundary line can pass through a preset area on the reaction surface.

[0025] In some embodiments, after the fixing structure fixes the sample carrier and the first supporting surface supports the first substrate, the second driving mechanism is configured to drive the fixing structure to move along the second direction during the process of the first substrate transferring the carried liquid to the sample carrier;

[0026] or,

[0027] After the fixing structure fixes the sample carrier and the first supporting surface supports the first substrate, the second driving mechanism is configured to drive the fixing structure to move in the opposite direction along the second direction during the process of the first substrate transferring the carried liquid to the sample carrier.

[0028] In some embodiments, the fixing structure is arranged above the first supporting portion;

[0029] or,

[0030] The fixing structure is arranged below the first supporting portion;

[0031] or,

[0032] The fixing structure and the first supporting portion are arranged in a horizontal direction.

[0033] In some embodiments, the liquid transfer device further comprises a liquid distributing device disposed upstream of the fixing portion, the liquid distributing device being configured to distribute the liquid onto the first substrate;

[0034] The liquid transfer device also includes a second supporting portion, which is arranged relative to the liquid distributing device at an interval, and the second supporting portion has a second supporting surface facing the liquid distributing device, and the second supporting surface is used to support the first substrate so that the liquid distributing device can distribute the liquid on the wall of the first substrate facing away from the second supporting surface.

[0035] In some embodiments, the liquid transfer device further comprises a liquid distributing device disposed upstream of the fixing portion, the liquid distributing device being configured to distribute the liquid onto the first substrate;

[0036] The liquid distributing device is arranged opposite to the first supporting surface with a distance therebetween, and the first supporting surface is used to support the first substrate, so that the liquid distributing device can distribute the liquid on the wall surface of the first substrate facing away from the first supporting surface.

[0037] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0038] The first supporting surface is a cylindrical surface, and the central axis of the cylindrical surface is parallel to the third direction, and the third direction is perpendicular to the first direction and the second direction;

[0039] The perpendicular line from the center of the liquid distributing device to the central axis is the first perpendicular line, and the perpendicular line from the center of the fixing part to the central axis is the second perpendicular line. When observed along the third direction, the angle between the first perpendicular line and the second perpendicular line is greater than or equal to ninety degrees.

[0040] In some embodiments, the liquid transfer device further comprises a bracket, wherein the bracket is respectively connected to the first supporting portion and the fixing portion;

[0041] The fixing portion is slidably connected to the bracket, and the fixing portion can slide until the sample carrier fixed to the fixing structure does not overlap with the first supporting surface along the first direction.

[0042] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0043] The fixing portion is configured to be slidable relative to the bracket along the second direction or the opposite direction of the second direction, and the fixing portion can slide until the sample carrier fixed to the fixing structure does not overlap with the first supporting surface along the first direction.

[0044] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0045] The first supporting surface is a cylindrical surface, and the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction;

[0046] The first support portion is configured to be rotatable relative to the bracket with the central axis serving as a rotation axis.

[0047] In some embodiments, the first supporting surface is planar, and the first supporting surface is arranged perpendicular to the first direction.

[0048] In some embodiments, the first supporting surface is configured to cover the reaction surface when viewed along the first direction after the fixing structure fixes the sample carrier.

[0049] In some embodiments, the first supporting surface is configured such that after the sample carrier is fixed by the fixing structure, the first supporting surface is located within the reaction surface when viewed along the first direction;

[0050] The liquid transfer device further includes a first driving mechanism, wherein the first driving mechanism is used to drive the first substrate to move along a second direction perpendicular to the first direction;

[0051] The liquid transfer device further includes a second driving mechanism connected to the fixed structure, and the second driving mechanism is configured to drive the fixed structure to reciprocate along the second direction or in the opposite direction of the second direction.

[0052] In some embodiments, the liquid transfer device also includes a third adjustment mechanism, which is connected to the first support part and can adjust the relative position of the first support part and the fixed part along the first direction, so that the first support part has at least two positioning positions with different distances from the fixed part.

[0053] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0054] The liquid transfer device also includes a fourth adjustment mechanism, which is connected to the first support part and can adjust the relative position between the first support part and the fixed part along the second direction or the opposite direction of the second direction, and the first support part can be adjusted by the fourth adjustment structure so that the sample carrier fixed to the fixed structure does not overlap with the first support surface along the first direction.

[0055] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0056] The liquid transfer device includes a plurality of first supporting parts, each of which includes a first supporting surface in the form of a cylinder, a central axis of each of the first supporting parts is parallel to a third direction, the third direction is perpendicular to the first direction and the second direction, respectively, and an end portion of each of the first supporting surfaces close to the fixing part is arranged along the second direction.

[0057] In some embodiments, the first support portions are arranged in abutment with each other along the second direction;

[0058] or,

[0059] The first support portions are spaced apart from each other along the second direction, and each of the first support portions is configured to be rotatable with its own central axis as a rotation axis.

[0060] In some embodiments, the liquid transfer device includes a plurality of fixing portions, each of which is arranged along a third direction perpendicular to the first direction, each of which is used to fix one of the sample carriers, and the first supporting surface at least partially faces each of the fixing portions;

[0061] The first supporting surface is configured to support a plurality of first substrates arranged along the third direction, and each of the first substrates is used one by one to transfer liquid between each of the sample carriers fixed to each of the fixing parts.

[0062] A second embodiment of the present invention provides a reaction device, comprising the liquid transfer device described in any of the above embodiments, the first substrate, and the sample carrier.

[0063] In some embodiments, the reaction device further includes a second substrate, which is used to replace the first substrate, and the thickness of the second substrate is greater than the thickness of the first substrate.

[0064] In some embodiments, the first substrate includes a first portion and a second portion distributed along its length direction, and a thickness of the first portion is greater than a thickness of the second portion.

[0065] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0066] The first supporting surface is a cylindrical surface, and the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction;

[0067] The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder, and the surrounding angle of the first substrate on the first supporting surface is greater than one hundred and eighty degrees.

[0068] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0069] The first supporting surface is a cylindrical surface, and the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction;

[0070] The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder. The central axis of the cylinder is perpendicular to the central plane, and the central plane coincides with the center line of the first substrate along its own length direction.

[0071] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being configured to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction;

[0072] The first supporting surface is a cylindrical surface, and the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction;

[0073] The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder, the angle between the central axis of the cylinder and the central surface is less than ninety degrees, and the central surface coincides with the center line of the substrate along its own length direction.

[0074] A third embodiment of the present invention provides an analysis device, comprising the reaction device and detection device described in any of the above embodiments.

[0075] A fourth aspect of the present invention provides a liquid transfer method using the reaction device described in any of the above embodiments to transfer liquid, the liquid transfer method comprising the following steps:

[0076] adjusting the fixing portion in a direction perpendicular to the first direction to a position that does not overlap with the first supporting surface in the first direction;

[0077] mounting the sample carrier to the fixed structure;

[0078] adjusting the fixing portion in a direction perpendicular to the first direction to a position where the first supporting surface faces the reaction surface;

[0079] The liquid carried by the substrate is transferred to the sample carrier.

[0080] In some embodiments, after the step of transferring the liquid carried by the substrate to the sample carrier, the method further includes the following steps:

[0081] adjusting the fixing portion in a direction perpendicular to the first direction to a position that does not overlap with the first supporting surface in the first direction;

[0082] Disassemble the sample carrier.

[0083] In some embodiments, after the step of adjusting the fixing portion in a direction perpendicular to the first direction to a position where the first supporting surface faces the reaction surface and before the step of transferring the liquid carried by the substrate to the sample carrier, the following step is further included:

[0084] The distance between the reaction surface and a wall surface of the first substrate facing away from the first supporting surface is adjusted along the first direction.

[0085] In some embodiments, the liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at the liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction; the first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; the first supporting surface has a boundary line arranged parallel to the third direction, and along the first direction, the boundary line is located at the end of the first supporting surface facing the fixed structure; the liquid transfer device further comprises a second driving mechanism, the second driving mechanism being connected to the fixed structure, and the second driving mechanism being configured to drive the fixed structure to reciprocate along the second direction or in the opposite direction of the second direction, so that after the fixed structure fixes the sample carrier, the boundary line can pass over a preset area on the reaction surface when observed along the first direction;

[0086] The step of transferring the liquid carried by the first substrate to the sample carrier includes:

[0087] driving the first substrate to move so that the liquid on the first substrate can contact the reaction surface;

[0088] During the process of the liquid on the first substrate contacting the reaction surface, the fixed part is driven to move in the opposite direction of the second direction so that the liquid on the first substrate can be transferred to the reaction surface; or, during the process of the liquid on the first substrate contacting the reaction surface, the fixed part is driven to move in the second direction so that the liquid on the first substrate can be transferred to the reaction surface.

[0089] In some embodiments, the first supporting surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction. The liquid transfer device further includes a first driving mechanism, the first driving mechanism being configured to drive the first supporting portion to rotate about the central axis of the cylindrical surface, thereby driving the first substrate to move. At the liquid exchange position between the first substrate and the sample carrier, the first supporting portion is configured to drive the first substrate to move in a second direction perpendicular to the first direction and the third direction.

[0090] The step of transferring the liquid carried by the substrate to the sample carrier comprises:

[0091] The first supporting portion is driven to rotate around the central axis to drive the first substrate to move.

[0092] In some embodiments, the reaction device further includes a second substrate, the second substrate is used to replace the first substrate, and the thickness of the second substrate is greater than the thickness of the first substrate;

[0093] The liquid transfer method further comprises the following steps:

[0094] removing the first substrate;

[0095] The second substrate is installed, wherein a thickness dimension of the liquid disposed on the first substrate is greater than a thickness dimension of the liquid disposed on the second substrate.

[0096] In some embodiments, the first substrate includes a first portion and a second portion distributed along its length direction, and the thickness of the first portion is greater than the thickness of the second portion;

[0097] The liquid transfer method further comprises the following steps:

[0098] disposing a first liquid on the first portion, the first liquid having a first thickness dimension;

[0099] disposing a second liquid on the second portion, the second liquid having a second thickness dimension; wherein the second thickness dimension is greater than the first thickness dimension;

[0100] Or the liquid transfer method further comprises the following steps:

[0101] disposing a first liquid on the first portion, the first liquid having a first thickness dimension;

[0102] A second liquid is disposed on the second portion, the second liquid having a second thickness dimension; wherein the second thickness dimension is equal to the first thickness dimension.

[0103] In some embodiments, the first substrate includes a first portion and a second portion distributed along its length direction, and the thickness of the first portion is greater than the thickness of the second portion;

[0104] The liquid transfer method further comprises the following steps:

[0105] disposing a first liquid on the first portion and not disposing a liquid on the second portion;

[0106] Or the liquid transfer method further comprises the following steps:

[0107] A second liquid is disposed on the second portion, and no liquid is disposed on the first portion.

[0108] Compared with the prior art, the present invention has the following beneficial effects:

[0109] In the technical solution of the present invention, the liquid transfer device can transfer the liquid carried by the first substrate to the sample carrier, so as to use the sample carrier to detect and analyze the liquid. The liquid transfer device includes a fixing part and a first supporting part. The fixing structure in the fixing part can fix the sample carrier to ensure the stability of the detection and analysis of the sample carrier. In this solution, the first supporting surface of the first supporting part at least partially faces the reaction surface of the sample carrier after the fixing structure fixes the sample carrier. That is, the first supporting surface can support the first substrate so that the liquid carried by the first substrate supported by the first supporting surface is stably transferred to the reaction surface of the sample carrier fixed by the fixing structure. Compared with the method in the prior art that relies on the first substrate itself to support the liquid and transfer the liquid to the sample carrier, the first supporting surface of the first supporting part in this solution can provide support for the first substrate, can effectively suppress the shaking of the first substrate during the liquid transfer process, prevent the first substrate from scratching the sample carrier, and evenly transfer the liquid to the sample carrier, thereby improving the stability of the liquid transfer. BRIEF DESCRIPTION OF THE DRAWINGS

[0110] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0111] FIG1 is a liquid transfer device according to a first embodiment of the present invention; wherein the fixing structure is located above the first supporting portion;

[0112] FIG2 is a liquid transfer device according to a second embodiment of the present invention, wherein the fixing structure is located below the first supporting portion;

[0113] FIG3 is a liquid transfer device according to a third embodiment of the present invention, wherein the fixed structure and the first support portion are arranged obliquely;

[0114] FIG4 is a liquid transfer device according to a fourth embodiment of the present invention; wherein the fixing structure is located on the left side of the first supporting portion;

[0115] FIG5 is a reaction device according to a fifth embodiment of the present invention; wherein the sample carrier is in a first positioning position relative to the first supporting surface;

[0116] FIG6 is a reaction device according to a fifth embodiment of the present invention; wherein the sample carrier is in a second positioning position relative to the first supporting surface;

[0117] FIG7 is a reaction device according to a sixth embodiment of the present invention; wherein the sample carrier and the first supporting surface overlap along the first direction X;

[0118] FIG8 is a reaction device according to a sixth embodiment of the present invention; wherein the sample carrier and the first supporting surface do not overlap along the first direction X;

[0119] FIG9 is a reaction device according to a seventh embodiment of the present invention; wherein the first supporting surface is a plane;

[0120] FIG10 is a reaction device according to an eighth embodiment of the present invention; wherein the first supporting surface is a curved surface and shows the liquid exchange portion;

[0121] FIG11 is a ninth embodiment of a reaction device according to the present invention; wherein the first driving mechanism drives the first substrate to move and the first supporting surface is a curved surface;

[0122] FIG12 is a reaction device according to a tenth embodiment of the present invention; wherein the first driving mechanism drives the first substrate to move and the first supporting surface is a cylindrical surface;

[0123] FIG13 is a reaction device according to an eleventh embodiment of the present invention; wherein the first driving mechanism drives the first supporting portion to rotate;

[0124] FIG14 is a reaction device according to a twelfth embodiment of the present invention, wherein the boundary line of the first substrate is shown;

[0125] FIG15 is a diagram of a reaction device according to a thirteenth embodiment of the present invention, showing a liquid distributing device and a plurality of supporting parts;

[0126] FIG16 is a reaction device according to a fourteenth embodiment of the present invention; wherein the first substrate partially surrounds the first support portion;

[0127] FIG17 is a reaction device according to a fifteenth embodiment of the present invention, wherein a support is shown;

[0128] FIG18 is a reaction device according to the sixteenth embodiment of the present invention; wherein the first support surface is flat and covers the reaction surface;

[0129] FIG19 is a reaction device according to a seventeenth embodiment of the present invention; wherein the first support surface is located within the reaction surface when viewed along the first direction;

[0130] FIG20 is a diagram of a reaction device according to an eighteenth embodiment of the present invention; wherein the third adjustment mechanism drives the first support portion to be in the third positioning position;

[0131] FIG21 is a diagram of a reaction device according to an eighteenth embodiment of the present invention; wherein the third adjustment mechanism drives the first support portion to the fourth positioning position;

[0132] FIG22 is a reaction device according to a nineteenth embodiment of the present invention; wherein the fourth adjustment mechanism adjusts and drives the sample carrier to coincide with the first support surface along the first direction X;

[0133] FIG23 is a reaction device according to the nineteenth embodiment of the present invention; wherein the fourth adjustment mechanism adjusts and drives the sample carrier and the first support surface to not overlap along the first direction X;

[0134] FIG24 is a reaction device according to the twentieth embodiment of the present invention, wherein a plurality of first support portions are in line contact and the first substrate is tensioned;

[0135] FIG25 is a reaction device according to the twenty-first embodiment of the present invention; wherein the plurality of first support portions are spaced apart from each other;

[0136] FIG26 is a reaction device according to a twenty-second embodiment of the present invention, wherein a first substrate and a second substrate are shown;

[0137] FIG27 is a reaction device according to a twenty-third embodiment of the present invention; wherein the first substrate includes a first portion and a second portion;

[0138] FIG28 is a reaction device according to a twenty-fourth embodiment of the present invention, wherein the first substrate surrounds the first support portion;

[0139] FIG29 is a diagram of a reaction device according to a twenty-fifth embodiment of the present invention; wherein the angle a1 between the central axis of the cylinder and the central plane is equal to ninety degrees;

[0140] FIG30 is a diagram of a reaction device according to a twenty-sixth embodiment of the present invention; wherein the angle a2 between the central axis of the cylinder and the central plane is less than 90 degrees;

[0141] FIG31 is a reaction device according to the twenty-seventh embodiment of the present invention; wherein the first supporting portion is provided with a first annular groove;

[0142] FIG32 is a reaction device according to the twenty-eighth embodiment of the present invention; wherein the first supporting portion supports two first substrates;

[0143] FIG33 is a diagram of a reaction device according to a twenty-ninth embodiment of the present invention, wherein a tension swing roller is shown;

[0144] FIG34 is an operational flow chart of a liquid transfer method according to a 30th embodiment of the present invention;

[0145] FIG35 is an operational flow chart of a liquid transfer method according to the thirty-first embodiment of the present invention.

[0146] DESCRIPTION OF REFERENCE NUMERALS: 1-reaction device; 10-liquid transfer device; 100-fixing portion; 110-fixing structure; 120-second perpendicular line; 200-first supporting portion; 210-first supporting surface; 211-arc surface; 212-axis line; 213-cylindrical surface; 2131-cylindrical surface; 214-central axis line; 215-boundary line; 216-plane surface; 220-first annular groove; 300-first adjustment mechanism; 400-second adjustment mechanism; 500-first driving mechanism; 600-second driving mechanism; 700-liquid dispensing device; 710-first perpendicular line; 800-second supporting portion; 810-second supporting surface; 900-bracket; 101-third adjustment mechanism; 102-fourth adjustment mechanism; 20-first substrate; 201 - liquid; 203 - first portion; 204 - second portion; 205 - center plane; 30 - sample carrier; 301 - reaction surface; 40 - second substrate; 50 - unwinding mechanism; 60 - rewinding mechanism; 70 - first tension oscillating roller; 80 - second tension oscillating roller; X - first direction; Y - second direction; Z - third direction.

[0147] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0148] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0149] In the related art, in order to reduce costs and increase throughput, a liquid transfer device is used to complete the transfer of liquid. Specifically, the film substrate is wound on a roller body, and a thin layer of reagent is coated on the surface of the film substrate using a liquid distributing device. At the liquid transfer position, the film substrate carrying the thin layer of reagent is driven by the roller body to move toward the sample carrier in a direction parallel to the reaction surface of the sample carrier. When the film substrate moves to the reaction surface of the sample carrier, the film substrate can transfer the carried thin layer of reagent to the sample carrier to achieve the transfer of liquid. However, because the film substrate is a flexible material, the film substrate will shake during the process of the film substrate carrying the thin layer of reagent moving toward the sample carrier, causing the film substrate to easily scratch the sample carrier, affecting the stability of the liquid transfer.

[0150] In view of this, with reference to Figures 1 to 33, the present invention proposes a liquid transfer device 10 for transferring the liquid 201 carried by the first substrate 20 to the sample carrier 30, which can ensure the stability of the transfer of the liquid 201. The liquid transfer device 10 can be used for gene sequencing, liquid 201 detection and analysis, etc. For ease of description and understanding, some embodiments of the present application take the liquid transfer device 10 for gene sequencing as an example. It should be noted that in gene sequencing, the first substrate 20 can be a film strip made of a flexible material (such as PET, etc.). The liquid 201 carried by the first substrate 20 can be a reaction reagent or a buffer reagent, etc. The reaction reagent is used to carry out biochemical reactions and achieve imaging to complete the gene sequencing operation. The buffer reagent is used to clean the reaction reagent to prevent mixing between different reagents, which can improve the accuracy of gene sequencing. It can be understood that the liquid 201 can be applied to one side of the first substrate 20 along its thickness direction.

[0151] The sample carrier 30 can detect and analyze the liquid 201. The sample carrier 30 of some embodiments of the present application is used for gene sequencing as an example. In gene sequencing, the sample carrier 30 refers to a sequencing chip. The specific layout of the sequencing chip can refer to relevant known technologies. It can be understood that the sample carrier 30 has a reaction surface 301. After the reaction reagent is transferred to the reaction surface 301, the reagent can undergo a biochemical reaction to achieve sequencing. Specifically, the liquid transfer device 10 of an embodiment of the present invention is introduced below with reference to Figures 1 to 4. The liquid transfer device 10 includes a fixing portion 100 and a first support portion 200.

[0152] The fixing portion 100 includes a fixing structure 110, which can fix the sample carrier 30. In some embodiments, the sample carrier 30 can be connected to the fixing structure 110 by adsorption to achieve fixation of the sample carrier 30. In other embodiments, the sample carrier 30 can also be connected to the fixing structure 110 by snapping to achieve fixation. In other embodiments, the sample carrier 30 can also be connected to the fixing structure 110 by threading to achieve fixation. The specific method can be determined according to actual conditions. In some embodiments of the present application, the sample carrier 30 is taken as an example of being connected to the fixing structure 110 by adsorption.

[0153] The first support portion 200 is used to support the first substrate 20 and can suppress vibration of the first substrate 20 during the process of transferring the liquid 201 to the sample carrier 30. The following describes the relative arrangement of the first support portion 200 and the fixed structure 110 during the transfer of the liquid 201, and defines a first direction X. The first support portion 200 and the fixed structure 110 are spaced apart and arranged relative to each other along the first direction X. The specific orientation of the first direction X can vary depending on the actual situation. In some embodiments, referring to the orientation shown in Figures 1 and 2, when the fixed structure 110 is located above or below the first support portion 200, the first direction X can refer to the upward or downward direction. In other embodiments, referring to the orientation shown in Figure 4, when the fixed structure 110 is located to the left or right of the first support portion 200, the first direction X can also refer to the left or right direction. In some embodiments, when the fixed structure 110 is located in front of or behind the first support portion 200 (not shown in the figures), the first direction X can also refer to the forward or backward direction. In other embodiments, with reference to the orientation shown in Figure 3, the first direction X may also refer to any inclined direction. For ease of description and understanding, the following embodiments take the first direction X as an upward direction as an example.

[0154] The first support portion 200 has a first support surface 210. It will be appreciated that, with reference to Figures 1 and 2 , in some embodiments, the first support surface 210 may be a flat surface 216. With reference to Figures 3 and 4 , in other embodiments, the first support surface 210 may also be a curved surface 211. The first support surface 210 is configured so that, when the fixing structure 110 secures the sample carrier 30, at least a portion faces the reaction surface 301 of the sample carrier 30. It will be appreciated that, when the fixing structure 110 secures the sample carrier 30, the first support surface 210 may partially or entirely face the sample carrier 30, depending on the specific circumstances. The meaning of the first support surface 210 facing the sample carrier 30 is explained below. The plane on which the reaction surface 301 lies is defined as the projection plane. The reaction surface 301 forms a first orthographic projection on the projection plane, and the first support surface 210 forms a second orthographic projection on the projection plane. When viewed along the first direction X, the first orthographic projection and the second orthographic projection overlap, indicating that the first support surface 210 faces the sample carrier 30.

[0155] 5 , the first supporting surface 210 of the first supporting portion 200 is used to support the first substrate 20 to prevent vibration of the first substrate 20. Specifically, the first supporting surface 210 can support the first substrate 20 along the first direction X, so that the liquid 201 carried by the first substrate 20 can be stably transferred to the reaction surface 301 of the sample carrier 30 secured by the securing structure 110, thereby enabling detection and analysis of the liquid 201 by the sample carrier 30.

[0156] It should be noted that in some embodiments, the liquid 201 coated on the first substrate 20 can directly contact the reaction surface 301 to achieve transfer of the liquid 201. In other embodiments, the liquid 201 coated on the first substrate 20 is spaced apart from the reaction surface 301. When the first support surface 210 is a flat surface 216 and the first substrate 20 moves relative to the sample carrier 30, a negative pressure is generated between the reaction surface 301 and the liquid 201, causing the liquid 201 to be adsorbed onto the reaction surface 301, achieving transfer of the liquid 201. In other embodiments, the liquid 201 coated on the first substrate 20 is spaced apart from the reaction surface 301. When the first support surface 210 is a curved surface 211 and the first substrate 20 moves relative to the sample carrier 30, the liquid 201 can be flung onto the reaction surface 301 along a tangent direction of the curved surface 211, achieving transfer of the liquid 201. The specific method of liquid transfer can be determined based on actual circumstances. In some embodiments of the present application, transfer of the liquid 201 is achieved by contacting the reaction surface 301 as an example.

[0157] In the technical solution of the present invention, the liquid transfer device 10 is capable of transferring the liquid 201 carried by the first substrate 20 to the sample carrier 30, so as to detect and analyze the liquid 201 using the sample carrier 30. The liquid transfer device 10 includes a fixing portion 100 and a first supporting portion 200. The fixing structure 110 in the fixing portion 100 is capable of fixing the sample carrier 30, ensuring the stability of the detection and analysis of the sample carrier 30. In this solution, the first supporting surface 210 of the first supporting portion 200 at least partially faces the reaction surface 301 of the sample carrier 30 after the fixing structure 110 fixes the sample carrier 30. That is, the first supporting surface 210 is capable of supporting the first substrate 20 so that the liquid 201 carried by the first substrate 20 supported by the first supporting surface 210 is stably transferred to the reaction surface 301 of the sample carrier 30 fixed by the fixing structure 110. Compared with the prior art method of relying on the first substrate itself to support the liquid and transfer the liquid to the sample carrier, the first supporting surface 210 of the first supporting portion 200 in this solution can provide support for the first substrate 20, thereby suppressing the shaking of the first substrate 20 during the transfer of the liquid 201, preventing the first substrate 20 from scratching the sample carrier 30, and ensuring that the liquid 201 is evenly transferred to the sample carrier 30, thereby improving the stability of the transfer of the liquid 201.

[0158] 5 and 6 , the liquid transfer device 10 further includes a first adjustment mechanism 300. The first adjustment mechanism 300 is connected to the fixed structure 110 and can adjust the relative position of the fixed structure 110 and the first support surface 210 along the first direction X. It will be understood that in some embodiments, the first adjustment mechanism 300 can actively adjust (the first adjustment mechanism 300 directly generates a driving force and adjusts) the fixed structure 110, that is, the first adjustment mechanism 300 is provided with a driving member, and the driving member can drive the fixed structure 110 to move relative to the first support portion 200. In other embodiments, the first adjustment mechanism 300 can also passively adjust (the first adjustment structure 300 passively obtains a driving force and then adjusts) the fixed structure 110, that is, an active member can be provided, the active member drives the first adjustment mechanism 300, and the first adjustment mechanism 300 drives the fixed structure 110 to move relative to the first support portion 200. In some embodiments, the relative position of the fixed structure 110 and the first support surface 210 can be manually adjusted. In other embodiments, the relative position of the fixed structure 110 and the first support surface 210 can also be electrically controlled (automatically adjusted). The specific settings may be determined according to actual conditions. Some embodiments of the present application take electronic control as an example.

[0159] In some embodiments, the first adjustment mechanism 300 can enable the sample carrier 30, which is fixed to the fixed structure 110, to have at least two positions with different distances from the first support surface 210 (in other embodiments, it can have three or more positions). Referring to Figure 5, in the first position, the sample carrier 30 can be separated from the first support surface 210 by a first distance D1, enabling the first substrate 20 supported by the first support surface 210 to transfer the liquid 201 onto the sample carrier 30 for detection and analysis.

[0160] 6 , in the second positioning position, the sample carrier 30 can be spaced apart from the first support surface 210 by a second distance D2 (the second distance being greater than the first distance), facilitating replacement of first substrates 20 of different thicknesses, transfer of liquids 201 of different thicknesses, or removal and assembly of the sample carrier 30. In other embodiments, the first adjustment mechanism 300 can further space the sample carrier 30 fixed to the fixed structure 110 apart from the first support surface 210 by other distances to accommodate first substrates 20 of other thicknesses, arrange layers of liquid 201 of other thicknesses, or facilitate removal and assembly of sample carriers 30 of different sizes.

[0161] It should be noted that the distance adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 can be linear, meaning that the sample carrier 30 moves at a constant speed relative to the first support surface 210. The distance adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 can also be nonlinear, meaning that the sample carrier 30 moves at a variable speed relative to the first support surface 210. Alternatively, the distance adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 can be continuous, meaning that the sample carrier 30 can move (slide or rotate) relative to the first support surface 210 and remain at any position. The distance adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 can also be stepwise, meaning that the sample carrier 30 can be adjusted to several specific positions relative to the first support surface 210. Specifically, the sample carrier 30 can move (slide or rotate) relative to the first support surface 210 and remain at several specific positions. The specific situation may depend on the actual situation. Some embodiments of the present application take linear regulation and continuous regulation as examples.

[0162] After the sample carrier 30 secured to the fixed structure 110 is spaced a predetermined second distance from the first support surface 210 along the first direction X using the first adjustment mechanism 300, the original sample carrier 30 can be removed and replaced with a new one (see FIG6 ). However, when transferring liquid 201 to the new sample carrier 30, this solution requires readjusting the gap between the new sample carrier 30 secured to the fixed structure 110 and the first support surface 210 along the first direction X to achieve the target size (first distance) suitable for liquid 201 transfer (see FIG5 ). Adjusting the gap between the new sample carrier 30 and the first support surface 210 is time-consuming and labor-intensive, impacting sequencing efficiency and accuracy. Therefore, in some embodiments, referring to FIG7 and FIG8 , the liquid transfer device 10 includes a second adjustment mechanism. The second adjustment mechanism is connected to the fixed structure 110 and is capable of adjusting the relative position of the fixed structure 110 and the first support surface 210 in a direction perpendicular to the first direction X to facilitate removal and replacement of the sample carrier 30. Since the minimum distance between the fixing structure 110 and the first support surface 210 along the first direction X does not change during the adjustment process of the fixing structure 110, this solution does not require re-adjustment of the gap between the new sample carrier 30 and the first support surface 210 after the fixing structure 110 is replaced, making the overall operation convenient and quick. It should be noted that the direction perpendicular to the first direction X mentioned above can also be the second direction Y or the third direction Z mentioned below.

[0163] The specific arrangement of the second adjustment mechanism is described below, which is similar to the first adjustment mechanism 300. In some embodiments, the second adjustment mechanism can actively adjust the fixed structure 110, that is, the second adjustment mechanism is provided with a driving member, and the driving member drives the fixed structure 110 to move relative to the first support portion 200. In other embodiments, the second adjustment mechanism can also passively adjust the fixed structure 110, that is, an active member can be provided, the active member drives the second adjustment mechanism, and the second adjustment mechanism drives the fixed structure 110 to move relative to the first support portion 200. In some embodiments, the relative position of the fixed structure 110 and the first support surface 210 can be manually adjusted. In other embodiments, the relative position of the fixed structure 110 and the first support surface 210 can also be automatically adjusted (electrically controlled). The specific settings can be determined according to actual conditions.

[0164] Referring to Figures 7 and 8, the fixing structure 110 can be adjusted by a second adjustment mechanism so that the sample carrier 30 fixed to the fixing structure 110 does not overlap with the first support surface 210 along the first direction X. To facilitate understanding of the meaning of "the sample carrier 30 does not overlap with the first support surface 210 along the first direction X," the plane on which the first support surface 210 lies is defined as the projection plane. The sample carrier 30 forms a third orthographic projection on the projection plane, and the first support surface 210 forms a fourth orthographic projection on the projection plane. "The sample carrier 30 does not overlap with the first support surface 210" means that the third and fourth orthographic projections do not overlap. Referring to Figure 7, when the sample carrier 30 fixed to the fixing structure 110 overlaps with the first support surface 210 along the first direction X, the liquid 201 carried by the first substrate 20 can be transferred to the sample carrier 30. Referring to Figure 8, when the sample carrier 30 fixed to the fixing structure 110 does not overlap with the first support surface 210 along the first direction X, the sample carrier 30 can be removed and replaced to prevent positional interference with the first support surface 210.

[0165] To facilitate description and understanding of the specific adjustment process of the second adjustment mechanism, the orientations shown in Figures 7 and 8 are used as reference for illustration. In some embodiments, the sample carrier 30 secured to the fixed structure 110 can move in the left-right direction (i.e., the second direction Y) so as to not overlap with the first support surface 210 along the first direction X. When the sample carrier 30 does not overlap with the first support surface 210, the sample carrier 30 can be located on the left or right side of the first support surface 210. In other embodiments, the sample carrier 30 secured to the fixed structure 110 can move in the front-to-back direction (i.e., the third direction Z) so as to not overlap with the first support surface 210 along the first direction X. When the sample carrier 30 does not overlap with the first support surface 210, the sample carrier 30 can be located in front of or behind the first support surface 210 (not shown in the figures), depending on the actual situation.

[0166] It is understood that, similar to the distance adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 along the first direction X (adjustment by the first adjustment mechanism 300), the position adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 along a direction perpendicular to the first direction X (adjustment by the second adjustment mechanism) can be linear or nonlinear. Furthermore, the position adjustment between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 along a direction perpendicular to the first direction X can be continuous or step-wise, depending on the actual situation.

[0167] Referring to Figures 9 and 10 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500 , which is configured to drive the first substrate 20 to move, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30 . To facilitate description and understanding of the specific movement of the first substrate 20 , a second direction Y is defined. The second direction Y is any direction perpendicular to the first direction X. With reference to the orientation in Figure 9 , illustratively, when the first direction X is vertical, the second direction Y is a horizontal direction perpendicular to the vertical direction; when the first direction X is horizontal, the second direction Y is a vertical direction perpendicular to the horizontal direction. For ease of understanding, the following example uses the horizontal left-right direction as an example.

[0168] At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first driving mechanism 500 is configured to drive the first substrate 20 to move along the second direction Y. It should be noted that the liquid 201 exchange position between the first substrate 20 and the sample carrier 30 varies on different first supporting surfaces 210. Referring to FIG9 , when the first supporting surface 210 is a flat surface 216, the liquid exchange position is any position on the first supporting surface 210 that can support contact between the liquid 201 and the reaction surface 301. Referring to FIG10 , when the first supporting surface 210 is a curved surface 211 (a cylindrical surface 2131 in FIG10 ), the liquid exchange point is a tangent line on the first supporting surface 210 that can support contact between the liquid 201 and the reaction surface 301. It should be noted that the tangent line is the line on the curved surface 211 closest to the reaction surface 301. When viewed in a direction parallel to the reaction surface 301, the liquid exchange point is a single point, namely, point A in FIG10 . To facilitate understanding and description of the specific motion state of the first substrate 20, the following description uses an example as a reference, viewed from a direction parallel to the reaction surface 301, with the fixing portion 100 and the supporting portion arranged vertically. In some embodiments, the first drive mechanism 500 can drive the first substrate 20 to move from left to right, from right to left, or reciprocate in the left-right direction. In other embodiments, the first drive mechanism 500 can drive the first substrate 20 to move from front to back, from back to front, or reciprocate in the front-to-back direction. In other embodiments, the first drive mechanism 500 can also drive the first substrate 20 to reciprocate unidirectionally or bidirectionally in other oblique directions. The specific operation depends on the actual situation. For ease of description, the orientation shown in Figure 10 is used as a reference, and the following example uses the first drive mechanism 500 driving the first substrate 20 to move unidirectionally from left to right.

[0169] Referring to Figures 9 and 10 , the second adjustment mechanism is configured to adjust the relative position of the fixed structure 110 and the first support surface 210 along the second direction Y or the opposite direction of the second direction Y to perform different operations. Specifically, the fixed structure 110 can be adjusted by the second adjustment mechanism to cause the sample carrier 30 fixed to the fixed structure 110 to overlap or misalign with the first support surface 210 along the first direction X. It will be appreciated that when the sample carrier 30 fixed to the fixed structure 110 overlaps with the first support surface 210 along the first direction X, the liquid 201 transfer operation can be performed, that is, the liquid 201 carried by the first substrate 20 can be transferred to the sample carrier 30. When the sample carrier 30 fixed to the fixed structure 110 misaligns with the first support surface 210 along the first direction X, the sample carrier 30 can be removed and replaced. In this embodiment, the first drive mechanism 500 drives the first substrate 20 to move, facilitating the transfer of the liquid 201 from the first substrate 20 to the sample carrier 30, thereby improving the uniformity and stability of the liquid 201 transfer. By adjusting the relative position of the fixed structure 110 and the first supporting surface 210 along the second direction Y or the opposite direction of the second direction Y by the second adjustment mechanism, there is no need to adjust the gap between the newly replaced sample carrier 30 and the first supporting surface 210 along the first direction X, which facilitates the disassembly and replacement of the sample carrier 30, avoids the first substrate 20 from scratching the sample carrier 30, and improves the detection efficiency and detection accuracy of the liquid 201.

[0170] Referring to Figure 11 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500 . The first drive mechanism 500 is used to drive the first substrate 20 to transfer the liquid 201 on the first substrate 20 to the sample carrier 30 . Similar to the above embodiments, to facilitate description and understanding of the specific movement of the first substrate 20 , a second direction Y is defined. The second direction Y is perpendicular to the first direction X. The specific arrangement of the first and second directions X and Y can be determined based on actual circumstances and will not be further described here.

[0171] At the liquid exchange position between the first substrate 20 and the sample carrier 30 , the first driving mechanism 500 is configured to drive the first substrate 20 to move along the second direction Y. It is understood that after being driven, the first substrate 20 can move unidirectionally along the second direction Y, or can also reciprocate bidirectionally along the second direction Y, so that the liquid 201 on the first substrate 20 can be completely transferred to the sample carrier 30 .

[0172] Referring to Figure 11 , the first supporting surface 210 can be a curved surface 211. To facilitate description and understanding of the specific placement of the curved surface 211, a third direction Z is defined. The third direction Z is perpendicular to the first direction X and the second direction Y, and the axis 212 of the curved surface 211 is parallel to the third direction Z. Using the orientation shown in Figure 10 as a reference, for example, when the first direction X is vertical, the second direction Y can be a horizontal front-to-back direction, and the third direction Z can be a horizontal left-to-right direction. When the first direction X is a horizontal left-to-right direction, the second direction Y can be a horizontal front-to-back direction, and the third direction Z can be a vertical direction. This configuration can be determined based on actual circumstances. It should be noted that the first supporting surface 210, which is a curved surface 211, can be a convex curved surface 211. When the first substrate 20 is supported on the convex curved surface 211 and transfers the liquid 201 to the sample carrier 30, line contact is established between the first substrate 20 and the sample carrier 30, enabling a uniform coating of the liquid 201 on the sample carrier 30. In this solution, the first supporting surface 210 with an arc surface 211 can tighten the first substrate 20, further improving the stability of the first supporting surface 210 supporting the first substrate 20, suppressing the shaking of the first substrate 20, preventing the first substrate 20 from scratching with the sample carrier 30, and ensuring the stability of the transfer of the liquid 201.

[0173] Referring to Figure 12 , the liquid transfer device 10 further includes a first drive mechanism 500. The first drive mechanism 500 is configured to drive the first substrate 20 to transfer the liquid 201 on the first substrate 20 to the sample carrier 30. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 to move in the second direction Y. Specifically, the first substrate 20 can be driven to move unidirectionally or bidirectionally in the second direction Y to ensure complete transfer of the liquid 201 on the first substrate 20 to the sample carrier 30.

[0174] Referring to Figure 12, the first supporting surface 210 is a cylindrical surface 213. In order to facilitate the description and understanding of the specific layout position of the first supporting surface 210, a third direction Z is defined. Similar to the arrangement of the first supporting surface 210 in the form of an arc surface 211, the third direction Z is perpendicular to the first direction X and the second direction Y, and the central axis 214 of the cylindrical surface 213 (the central axis in Figure 12 is a point) is parallel to the third direction Z. It should be noted that the cylindrical surface 213 can be a square cylindrical surface 213, a circular cylindrical surface 213, an elliptical cylindrical surface 213, etc. The cylindrical surface 2131 is used as an example for explanation below. In this solution, the first supporting surface 210 in the form of a cylindrical surface 213 can further tighten the first substrate 20, enhance the stability of the first supporting surface 210 supporting the first substrate 20, further suppress the shaking of the first substrate 20, prevent the first substrate 20 from scratching with the sample carrier 30, and ensure the stability of the transfer of the liquid 201.

[0175] Referring to Figure 12, in some embodiments, based on the liquid transfer device 10 being provided with a first drive mechanism 500, the cylindrical surface 213 of the first support surface 210 is a cylindrical surface 2131. The first support surface 210 arranged as a cylindrical surface 2131 can effectively tighten the first substrate 20, further suppressing vibration of the first substrate 20, and improving the stability of the transfer of the liquid 201. The first support portion 200 is configured to be driven by the first substrate 20 and rotate about the central axis 214 of the cylindrical surface 2131. That is, the first support portion 200 can both support the first substrate 20 and rotate as the first substrate 20 slides. Therefore, friction loss between the first substrate 20 and the first support portion 200 can be reduced, allowing the first substrate 20 to be recycled multiple times, ensuring that the liquid 201 transfer operation can continue normally.

[0176] Referring to Figure 13 , in some embodiments, the first supporting surface 210 is a cylindrical surface 2131, meaning that the first supporting portion 200 comprises a roller capable of supporting the first substrate 20. To facilitate description and understanding of the specific arrangement of the cylindrical surface 2131, a third direction Z is defined. The central axis 214 of the cylindrical surface 2131 (the central axis in Figure 13 is a point) is parallel to the third direction Z, which is perpendicular to the first direction X.

[0177] The liquid transfer device 10 also includes a first drive mechanism 500. The first drive mechanism 500 is used to drive the first support portion 200 to rotate about the central axis 214 of the cylindrical surface 213, thereby driving the movement of the first substrate 20. As can be understood, referring to FIG. 13 , when the first drive mechanism 500 drives the first support portion 200 to rotate clockwise, the first substrate 20 slides from left to right. When the first drive mechanism 500 drives the first support portion 200 to rotate counterclockwise, the first substrate 20 slides from right to left.

[0178] To facilitate description and understanding of the specific motion trajectory of the first substrate 20, the second direction Y is defined as perpendicular to the first direction X and the third direction Z. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first support portion 200 is configured to drive the first substrate 20 to move along the second direction Y. Specifically, the first drive mechanism 500 rotates the first support portion 200. The rotating first support portion 200, relying on tension and friction with the first substrate 20, drives the first substrate 20 to move along the second direction Y, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30 for detection.

[0179] In this solution, the first driving mechanism 500 drives the first support part 200 to rotate. The way in which the first support part 200 drives the first substrate 20 to move can further improve the stability of the movement of the first substrate 20, reduce the vibration amplitude of the first substrate 20, prevent the first substrate 20 from scratching the sample carrier 30, and ensure the stability and reliability of the detection of the sample carrier 30.

[0180] In some embodiments, the cylindricity fluctuation dR of the cylindrical surface 2131 is ≤ 0.0003 mm. For example, the cylindricity fluctuation can be 0.0003 mm, 0.0002 mm, or 0.0001 mm, etc. It should be noted that the parameter arrangement of the cylindricity fluctuation in this solution can ensure the stability of the movement of the first substrate 20 driven by the first support portion 200, allowing the liquid 201 on the first substrate 20 to be stably transferred to the sample carrier 30, preventing the first substrate 20 from scratching the sample carrier 30, and ensuring the accuracy of the detection and analysis of the sample carrier 30. It also facilitates the arrangement of the various structures of the liquid transfer device 10, reduces the difficulty of matching and processing each part, and saves production costs.

[0181] Referring to Figure 14 , in some embodiments, the first support surface 210 has a boundary line 215 arranged parallel to the third direction Z. It will be understood that the length of the boundary line 215 is the extension length of the first support surface 210 along the third direction Z. When viewed along the first direction X, the boundary line 215 is located at the end of the first support surface 210 facing the fixed structure 110. For example, when the fixed structure 110 is disposed at the upper end of the first support portion 200, the boundary line 215 is the upper edge of the first support surface 210. When the fixed structure 110 is disposed at the left end of the first support portion 200, the boundary line 215 is the left edge of the first support surface 210.

[0182] The liquid transfer device 10 further includes a second drive mechanism 600, which is used to drive the fixed structure 110. The second drive mechanism 600 is connected to the fixed structure 110. It is understood that the second drive mechanism 600 can be directly connected to the fixed structure 110 or indirectly connected to the fixed structure 110 via the fixing portion 100, depending on the actual situation.

[0183] The second drive mechanism 600 is capable of driving the fixed structure 110 to reciprocate along the second direction Y or in the opposite direction of the second direction Y to evenly apply the liquid 201 on the first substrate 20 to the reaction surface 301 of the sample carrier 30. After the fixed structure 110 secures the sample carrier 30, the boundary line 215 can pass through a predetermined area on the reaction surface 301 as viewed along the first direction X. It should be noted that the liquid 201 on the boundary line 215 can directly contact the reaction surface 301 to transfer the liquid 201 to the sample carrier 30. It should be understood that the predetermined area refers to the area on the reaction surface 301 where the liquid 201 can be detected and analyzed. The size of the predetermined area can be determined based on actual conditions. In this solution, utilizing the boundary line 215 of the first support surface 210 to transfer the liquid 201 effectively reduces the contact area between the first substrate 20 and the sample carrier 30, preventing the first substrate 20 from scratching the sample carrier 30 and ensuring the stability of the liquid 201 transfer.

[0184] In some embodiments, after the fixed structure 110 secures the sample carrier 30 and the first support surface 210 supports the first substrate 20, i.e., when preparations for transferring the liquid 201 are complete, the second drive mechanism 600 is configured to drive the fixed structure 110 along the second direction Y during the process of transferring the liquid 201 carried by the first substrate 20 to the sample carrier 30, as shown in FIG10 . With reference to the orientation shown in FIG10 , for example, when the fixed structure 110 and the first support portion 200 are arranged in a vertical direction (first direction X), the second drive mechanism 600 can drive the fixed structure 110 to move horizontally from left to right (second direction Y) to transfer the liquid 201. When the fixed structure 110 and the first support portion 200 are arranged in a horizontal direction, the second drive mechanism 600 can drive the fixed structure 110 to move vertically from bottom to top to transfer the liquid 201.

[0185] It will be appreciated that, similar to the above-described embodiment, after the fixed structure 110 secures the sample carrier 30 and the first support surface 210 supports the first substrate 20, the second drive mechanism 600 is configured to drive the fixed structure 110 to move in the opposite direction of the second direction Y during the process of the first substrate 20 transferring the carried liquid 201 to the sample carrier 30. With reference to the orientation shown in FIG10 , for example, when the fixed structure 110 and the first support portion 200 are arranged vertically (the first direction X), the second drive mechanism 600 can drive the fixed structure 110 to move horizontally from right to left (the opposite direction of the second direction Y) to transfer the liquid 201. When the fixed structure 110 and the first support portion 200 are arranged horizontally, the second drive mechanism can drive the fixed structure 110 to move vertically from top to bottom to transfer the liquid 201. The specific driving process of the second drive mechanism 600 can be determined based on actual conditions.

[0186] In this solution, the second driving mechanism 600 can drive the fixed structure 110 to move along the second direction Y or the reverse direction Y during the transfer of the liquid 201, which can not only improve the transfer rate of the liquid 201 and fully utilize the liquid 201, but also enable the liquid 201 to be evenly coated on the reaction surface 301 of the sample carrier 30, thereby ensuring the accuracy and stability of the detection of the liquid 201.

[0187] Referring to Figure 15 , in some embodiments, the liquid transfer device 10 further includes a liquid distributing device 700 disposed upstream of the fixed portion 100. It should be noted that the placement of the liquid distributing device 700 upstream of the fixed portion 100 indicates that the liquid distributing process occurs before the transfer of the liquid 201. Specifically, the liquid distributing device 700 first distributes the liquid to the first substrate 20, and then the first substrate 20 carries the liquid 201 and transfers the liquid 201 to the sample carrier 30. The liquid distributing device 700 is used to distribute the liquid 201 onto the first substrate 20, thereby transferring the liquid 201 through the first substrate 20 to the reaction surface 301 of the sample carrier 30 for detection and analysis.

[0188] The liquid transfer device 10 also includes a second support portion 800, which is spaced apart from and arranged opposite to the liquid dispensing device 700. The second support portion 800 has a second support surface 810 facing the liquid dispensing device 700. The second support surface 810 is used to support the first substrate 20, so that the liquid dispensing device 700 can place the liquid 201 on the wall of the first substrate 20 facing away from the second support surface 810. In this embodiment, the second support portion 800 can be spaced apart from and arranged opposite to the liquid dispensing device 700 along a first direction X. That is, when the first substrate 20 moves along a second direction Y perpendicular to the first direction X, it can be supported by both the first support portion 200 and the second support portion 800, further improving the stability of the movement of the first substrate 20 and effectively suppressing the shaking of the first substrate 20. In addition, using the liquid dispensing device 700 to apply the liquid 201 to the first substrate 20 can not only ensure the uniformity of the applied liquid 201 layer, but also facilitate the placement of a specified thickness of liquid 201, achieve accurate detection of the liquid 201, reduce liquid 201 loss, and reduce detection costs.

[0189] Referring to Figure 16 , in some embodiments, the liquid transfer device 10 further includes a liquid distributing device 700 disposed upstream of the fixing portion 100. The placement of the liquid distributing device 700 upstream of the fixing portion 100 has the same meaning as described above and is not further described here. The liquid distributing device 700 is used to distribute the liquid 201 onto the first substrate 20, thereby transferring the liquid 201 through the first substrate 20 to the reaction surface 301 of the sample carrier 30 for detection and analysis.

[0190] The liquid distributing device 700 is spaced apart from and arranged opposite to the first support surface 210. The first support surface 210 is used to support the first substrate 20. The first substrate 20 partially surrounds the first support portion 200, allowing the first support portion 200 to pull the first substrate 20, further suppressing the shaking of the first substrate 20. The liquid distributing device 700 is capable of distributing the liquid 201 on the wall surface of the first substrate 20 facing away from the first support surface 210. The following briefly describes the process of transferring the liquid 201. First, the liquid distributing device 700 distributes the liquid 201 on the first substrate 20. The first substrate 20 carries the liquid 201 and moves along a second direction Y perpendicular to the first direction X. After the liquid 201 on the first substrate 20 contacts the sample carrier 30, the liquid 201 is transferred to the reaction surface 301 of the sample carrier 30 to achieve detection and analysis. The first support portion 200 of this solution is capable of supporting the first substrate 20, so that the first substrate 20 can stably support the liquid 201 from the liquid distributing device 700 and can stably transfer the liquid 201 to the sample carrier 30.

[0191] In some embodiments, the liquid transfer device 10 further includes a first driving mechanism 500, which is used to drive the first substrate 20 to move. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first driving mechanism 500 is configured to drive the first substrate 20 to move along a second direction Y that is perpendicular to the first direction X. It should be noted that at the liquid exchange position separating the first substrate 20 and the sample carrier 30, the first substrate 20 can move along the second direction Y or along other directions. It is understood that when the first supporting surface 210 is a cylindrical surface 213 (i.e., the first supporting portion 200 is roller-shaped), the first substrate 20 moves along the tangent direction of the cylindrical surface 213 at the separated position.

[0192] The first support surface 210 is a cylindrical surface 213, meaning that the outer contour of the first support portion 200 is roller-shaped. To facilitate description and understanding of the specific arrangement of the first support portion 200, the first support surface 210, which is a cylindrical surface 213, is defined as having a central axis 214, which is parallel to the extension direction of the first support portion 200. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z. The third direction Z is perpendicular to the first direction X and the second direction Y. In other words, the arrangement direction of the first support portion 200 is perpendicular to the first direction X and the second direction Y. This facilitates the rotation of the first support portion 200, which drives the first substrate 20 along the second direction Y, thereby stably transferring the liquid 201 to the sample carrier 30.

[0193] To facilitate description and understanding of the specific arrangement of the liquid distributing device 700 and the fixing unit 100, referring to Figure 16 , a line perpendicular from the center of the liquid distributing device 700 to the central axis 214 is defined as a first perpendicular line 710, and a line perpendicular from the center of the fixing unit 100 to the central axis 214 is defined as a second perpendicular line 120. When viewed along the third direction Z, the angle between the first perpendicular line 710 and the second perpendicular line 120 is greater than or equal to 90 degrees, indicating that the liquid distributing device 700 and the fixing unit 100 can tension the first substrate 20, effectively suppressing vibration of the first substrate 20 and ensuring stable transfer of the liquid 201.

[0194] 17 , in some embodiments, the liquid transfer device 10 further includes a bracket 900. The bracket 900 is used to support the first support portion 200 and the fixing portion 100. The bracket 900 connects the first support portion 200 and the fixing portion 100, respectively, thereby connecting the first support portion 200 and the fixing portion 100 into a single unit. This improves the integration of the liquid transfer device 10 and facilitates modular configuration of the various components of the liquid transfer device 10.

[0195] The fixing portion 100 is slidably connected to the bracket 900. Specifically, the fixing portion 100 can slide relative to the bracket 900 along the second direction Y, along the third direction Z, or along other directions perpendicular to the first direction X. It will be appreciated that the specific sliding movement of the fixing portion 100 and the bracket 900 depends on the arrangement of the associated sliding structure. The fixing portion 100 can slide until the sample carrier 30 fixed to the fixing structure 110 is not aligned with the first support surface 210 along the first direction X, thereby facilitating the removal and replacement of the sample carrier 30 and meeting the requirements for liquid 201 testing in different scenarios.

[0196] It should be noted that in some embodiments, a slide rail can be arranged on the bracket 900, and a slider can be arranged on the fixed part 100, so that the fixed part 100 can slide in the slide rail provided on the bracket 900 through the slider. In other embodiments, a fixed block can be arranged on the bracket 900, and a slide rail can be arranged on the fixed part 100, so that the fixed part 100 can slide in the fixed block provided on the bracket 900 through the slide rail. In other embodiments, a rotating wheel can also be arranged on the bracket 900, and a belt can be provided on the fixed part 100, so that the fixed part 100 can slide relative to the rotating wheel of the bracket 900 through the belt. The specific arrangement of the sliding structure can be determined according to actual conditions. It can be understood that a limiting structure can also be provided at a specified position on the slide rail to limit the distance and trajectory of the movement of the fixed part 100 relative to the bracket 900.

[0197] In this embodiment, the bracket 900 connects the first support portion 200 and the fixing portion 100 into a single unit. After the first substrate 20 on the first support portion 200 completes the transfer of the liquid 201, the fixing portion 100 slides relative to the bracket 900 to displace the sample carrier 30 secured to the fixing structure 110 from the first support surface 210 along the first direction X, thereby enabling assembly and disassembly of the sample carrier 30. The overall operation of the liquid transfer device 10 is convenient and fast, improving the efficiency of assembly and disassembly of the sample carrier 30 and preventing the sample carrier 30 from interfering with or colliding with the first support portion 200, thereby ensuring stable detection of the liquid 201.

[0198] Referring to FIG. 17 , in some embodiments, the liquid transfer device 10 is provided with a support 900 . Furthermore, the liquid transfer device 10 further includes a first drive mechanism 500 , which is configured to drive the first substrate 20 to move. At the liquid exchange position between the first substrate 20 and the sample carrier 30 , the first drive mechanism 500 is configured to drive the first substrate 20 to move in a second direction Y perpendicular to the first direction X. It should be noted that at positions spaced from the liquid exchange position between the first substrate 20 and the sample carrier 30 , the first substrate 20 can move in the second direction Y or other directions. Using the orientation shown in FIG. 17 as a reference, illustratively, when the first support surface 210 is a cylindrical surface 213 (i.e., the first support portion 200 is roller-shaped), the first substrate 20 at this spaced position (spaced from the liquid exchange position) moves in a tangential direction of the cylindrical surface 213 . When the first support surface 210 is a flat surface 216 , the first substrate 20 at this spaced position (spaced from the liquid 201 exchange position) moves in the second direction Y.

[0199] The fixing portion 100 is configured to slide relative to the bracket 900 along the second direction Y or the opposite direction of the second direction Y. The fixing portion 100 can slide until the sample carrier 30 fixed to the fixing structure 110 and the first supporting surface 210 do not overlap along the first direction X. In other words, when viewed from the reaction surface 301 of the sample carrier 30, the first supporting surface 210 does not block the sample carrier 30, thereby facilitating removal and replacement of the sample carrier 30.

[0200] In this embodiment, the fixing portion 100 can slide relative to the bracket 900 until the sample carrier 30 fixed by the fixing structure 110 and the first support portion 200 do not overlap along the first direction X. This facilitates the installation and removal of the sample carrier 30. The arrangement of the bracket 900 ensures the stability of the connection between the first support portion 200 and the fixing portion 100. The first driving mechanism 500 can drive the movement of the first substrate 20, and the first support portion 200 can support the first substrate 20, further ensuring the stability of the transfer of the liquid 201.

[0201] 17 , the liquid transfer device 10 further includes a first drive mechanism 500 for driving the first substrate 20 to move. At a liquid exchange point between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 to move in a second direction Y perpendicular to the first direction X. It will be appreciated that at locations spaced apart from the liquid exchange point between the first substrate 20 and the sample carrier 30, the first substrate 20 may move in the second direction Y or in other directions.

[0202] The first support surface 210 of the first support portion 200 is a cylindrical surface 213, meaning that the outer contour of the first support portion 200 is roller-shaped. For ease of description and understanding, the first support surface 210, which is a cylindrical surface 213, is defined as having a central axis 214, which is parallel to the extension direction of the first support portion 200. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z, which is perpendicular to the first direction X and the second direction Y. In other words, the arrangement of the first support portion 200 is perpendicular to the first direction X and the second direction Y, facilitating rotation of the first support portion 200 with the first substrate 20 along the second direction Y, thereby stably transferring the liquid 201 to the sample carrier 30. The first support portion 200 is configured to rotate relative to the support 900 about the central axis 214 as the rotation axis 212, thereby stably supporting the first substrate 20 driven by the first drive mechanism 500.

[0203] In this embodiment, the fixing portion 100 can slide relative to the bracket 900 until the sample carrier 30 secured by the fixing structure 110 and the first support portion 200 do not overlap along the first direction X, facilitating assembly and disassembly of the sample carrier 30. The arrangement of the bracket 900 ensures the stability of the connection between the first support portion 200 and the fixing portion 100. The first driving mechanism 500 is used to drive the movement of the first substrate 20, and the first support surface 210 of the first support portion 200 is a cylindrical surface 213. When the first substrate 20 is driven to move in a second direction Y perpendicular to the first direction X, the first support portion 200 can rotate relative to the bracket 900 about its central axis 214. This ensures that the first support portion 200 provides continuous support for the first substrate 20 and reduces frictional resistance between the first substrate 20 and the first support surface 210, further suppressing vibration of the first substrate 20 and ensuring stable transfer of the liquid 201.

[0204] Referring to Figure 18 , the first support surface 210 of the first support portion 200 is flat 216 . In some embodiments, the first support surface 210 can be arranged perpendicular to the first direction X. In other embodiments, the first support surface 210 can be arranged obliquely relative to the first direction X. The specific arrangement can be determined based on actual circumstances. The flat 216 arrangement of the first support surface 210 can increase the contact area with the first substrate 20 , stably support the first substrate 20 , effectively control the vibration amplitude of the first substrate 20 , and prevent the first substrate 20 from scratching the sample carrier 30 .

[0205] Referring to Figure 18 , in some embodiments, the first support surface 210 is a plane 216. The first support surface 210 is configured such that, after the sample carrier 30 is secured by the fixing structure 110, when viewed along the first direction X, the first support surface 210 covers the reaction surface 301. To facilitate description and understanding of the relative sizes of the first support surface 210 and the reaction surface 301, assume that the plane on which the reaction surface 301 lies is a projection plane. The reaction surface 301 is defined as forming a first projection area on the projection plane, and the first support surface 210 is defined as forming a second projection area on the projection plane. When viewed along the first direction X, the fact that the first support surface 210 covers the reaction surface 301 can be understood as meaning that the first projection area falls within the second projection area. In other words, the first projection area is smaller than or equal to the second projection area, meaning that the reaction surface 301 is smaller than or equal to the first support surface 210. By making the reaction surface 301 smaller than the first support surface 210, the liquid 201 is uniformly coated on the reaction surface 301 of the sample carrier 30, ensuring accurate testing of the sample carrier 30.

[0206] Referring to Figure 19 , in some embodiments, the first support surface 210 is a plane 216. The first support surface 210 is configured such that, after the sample carrier 30 is secured by the securing structure 110, when viewed along the first direction X, the first support surface 210 is located within the reaction surface 301. It should be noted that the explanation regarding the relative sizes of the first support surface 210 and the reaction surface 301 is similar to that in the above-described embodiments and will not be repeated here. Specifically, when viewed along the first direction X, the first support surface 210 is smaller than or equal to the reaction surface 301.

[0207] The liquid transfer device 10 further includes a first driving mechanism 500 , which is configured to drive the first substrate 20 to move along a second direction Y perpendicular to the first direction X, so as to transfer the liquid 201 on the first substrate 20 to the sample carrier 30 .

[0208] The liquid transfer device 10 also includes a second driving mechanism 600, which is connected to the fixed structure 110 and is configured to drive the fixed structure 110 to reciprocate along the second direction Y or the opposite direction of the second direction Y, so that the liquid 201 on the first substrate 20 can be evenly coated on the entire reaction surface 301 of the sample carrier 30.

[0209] In this embodiment, the first support surface 210 is smaller than or equal to the reaction surface 301. This improves the utilization rate of the liquid 201 carried by the first substrate 20 supported by the first support surface 210, reduces the amount of liquid 201 used, and lowers production costs. The first drive mechanism 500 drives the first substrate 20 to move, while the second drive mechanism 600 drives the fixed structure 110 to reciprocate in the second direction Y or in the opposite direction of the second direction Y. This ensures that the liquid 201 on the first substrate 20 is evenly transferred to the sample carrier 30, ensuring the accuracy and stability of the detection and analysis performed by the sample carrier 30.

[0210] When the distance between the sample carrier 30 (fixed to the fixed structure 110) and the first support portion 200 is adjusted only by the first adjustment mechanism 300, the overall adjustable stroke is limited. Referring to Figures 20 and 21, in some embodiments, the liquid transfer device 10 further includes a third adjustment mechanism 101. The third adjustment mechanism 101 is connected to the first support portion 200 and is capable of adjusting the relative position of the first support portion 200 and the fixed portion 100 along the first direction X, thereby increasing the adjustment stroke of the first support portion 200 and the fixed portion 100 along the first direction X. It will be understood that in some embodiments, the third adjustment mechanism 101 can actively adjust the first support portion 200, that is, the third adjustment mechanism 101 is provided with a driving member that can drive the first support portion 200 to move relative to the fixed portion 100. In other embodiments, the third adjustment mechanism 101 can also passively adjust the first support portion 200, that is, an active member can be provided, and the active member drives the third adjustment mechanism 101, and the third adjustment mechanism 101 drives the first support portion 200 to move relative to the fixed portion 100. In some embodiments, the relative position of the first support portion 200 and the fixing portion 100 can be manually adjusted. In other embodiments, the relative position of the first support portion 200 and the fixing portion 100 can also be automatically adjusted (electrically controlled). The specific setting can be determined according to actual conditions.

[0211] The third adjustment mechanism 101 enables the first support portion 200 to have at least two different positioning positions relative to the fixed portion 100. Referring to FIG20 , in the third positioning position, the fixed portion 100 can be spaced a third distance from the first support portion 200 (the third distance can be the same as or different from the first distance), enabling the first substrate 20 supported by the first support surface 210 of the first support portion 200 to transfer liquid 201 to the sample carrier 30 for detection and analysis. Referring to FIG21 , in the fourth positioning position, the sample carrier 30 can be spaced a fourth distance from the first support surface 210 (the fourth distance is greater than the third distance and can be the same as or different from the second distance), facilitating the replacement of first substrates 20 of different thicknesses or the transfer of liquids 201 of different thicknesses. In other embodiments, the third adjustment mechanism 101 can also space the first support portion 200 from the fixed portion 100 at other distances to accommodate first substrates 20 of different thicknesses or to deposit layers of liquid 201 of different thicknesses. It is understood that the distance adjustment between the first support portion 200 and the fixing portion 100 can be linear or nonlinear. In addition, the distance adjustment between the first support portion 200 and the fixing portion 100 can be continuous or stepped, depending on the actual situation.

[0212] 22 and 23 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500, which is configured to drive the first substrate 20 to move, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30. To facilitate description and understanding of the specific movement of the first substrate 20, a second direction Y is defined. The second direction Y is perpendicular to the first direction X.

[0213] At the liquid exchange point between the first substrate 20 and the sample carrier 30, the first driving mechanism 500 is configured to drive the first substrate 20 to move along the second direction Y. It should be noted that the location of the liquid exchange point between the first substrate 20 and the sample carrier 30 varies on different first supporting surfaces 210. When the first supporting surface 210 is a flat surface 216, the liquid exchange point is any location on the first supporting surface 210 that can support contact between the liquid 201 and the reaction surface 301. When the first supporting surface 210 is a curved surface 211, the liquid exchange point is a tangent line on the first supporting surface 210 that can support contact between the liquid 201 and the reaction surface 301.

[0214] The liquid transfer device 10 further includes a fourth adjustment mechanism 102, which is used to adjust the relative position of the first support portion 200 and the fixing portion 100 along the second direction Y or the opposite direction of the second direction Y to facilitate removal and replacement of the sample carrier 30. The fourth adjustment mechanism 102 is connected to the first support portion 200. It will be appreciated that the fourth adjustment mechanism 102 can be directly connected to the first support portion 200 or indirectly connected to the first support portion 200 via other components.

[0215] The following describes the specific arrangement of the fourth adjustment mechanism 102. The fourth adjustment mechanism 102 can actively or passively adjust the first support portion 200. In other embodiments, the relative position of the first support portion 200 and the fixing portion 100 can be adjusted manually or automatically (electronically). The specific arrangement can be determined based on actual conditions.

[0216] 23 , the first support portion 200 can be adjusted by the fourth adjustment structure so that the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 do not overlap along the first direction X (same meaning as above). It should be noted that, referring to FIG. 22 , when the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 overlap along the first direction X, the liquid 201 carried by the first substrate 20 can be transferred to the sample carrier 30. When the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 do not overlap along the first direction X, the sample carrier 30 can be installed and removed.

[0217] In this embodiment, the first drive mechanism 500 drives the first substrate 20 to move, evenly coating the liquid 201 on the first substrate 20 onto the sample carrier 30 and ensuring stable transfer of the liquid 201. The fourth adjustment mechanism 102 adjusts the relative position of the fixed structure 110 and the first support surface 210 along the second direction Y or the opposite direction of the second direction Y. This eliminates the need to adjust the gap between the newly replaced sample carrier 30 and the first support surface 210 along the first direction X, facilitating the removal and replacement of the sample carrier 30 and preventing the first substrate 20 from scratching the sample carrier 30, thereby improving the efficiency and accuracy of liquid 201 detection.

[0218] 24 and 25 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500. The first drive mechanism 500 is used to drive the first substrate 20 to move, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30. To facilitate description and understanding of the specific movement of the first substrate 20, a second direction Y is defined. The second direction Y is any direction perpendicular to the first direction X.

[0219] At the liquid exchange position between the first substrate 20 and the sample carrier 30 , the first driving mechanism 500 is configured to drive the first substrate 20 to move along a second direction Y perpendicular to the first direction X. The explanation of the liquid exchange position is similar to that above and will not be repeated here.

[0220] The following describes the motion of the first substrate 20. Referring to the orientation shown in FIG. 25 , the first drive mechanism 500 can, for example, drive the first substrate 20 from right to left. Alternatively, the first drive mechanism 500 can drive the first substrate 20 from front to back.

[0221] 24 and 25 , the liquid transfer device 10 includes a plurality of first support portions 200 , each of which is used to support a first substrate 20 , further improving the stability of the first substrate 20 and suppressing vibration of the first substrate 20 during the transfer of liquid 201. Each first support portion 200 includes a first support surface 210 in the form of a cylindrical surface 213 , meaning that each first support portion 200 has a roller-shaped profile, providing more balanced support for the first substrate 20. It will be appreciated that the structures and profiles of each first support portion 200 may be identical or different; in some embodiments of this application, the first support portions 200 are identical.

[0222] To facilitate description and understanding of the specific positional arrangement of each first support portion 200, a third direction Z is defined, which is perpendicular to both the first direction X and the second direction Y. The central axis of each first support portion 200 is parallel to the third direction Z, that is, the first support portions 200 can be arranged along the second direction Y. Specifically, the end of each first support surface 210 close to the fixing portion 100 is arranged along the second direction Y.

[0223] In this solution, the liquid transfer device 10 is provided with multiple first support parts 200 to jointly support the first substrate 20, which can not only enable the first substrate 20 to move more stably toward the sample carrier 30 after being driven, suppress the shaking of the first substrate 20, and prevent the first substrate 20 from scratching the sample carrier 30, but also meet the support requirements of first substrates 20 of various specifications and adapt to various liquid 201 transfer scenarios.

[0224] 24 and 25 , the liquid transfer device 10 is provided with a plurality of first support portions 200. In some embodiments, the first support portion 200 is roller-shaped, and the first support surface 210 is a cylindrical surface 213. The first support portions 200 can be arranged in abutment along the second direction Y, that is, there is line contact between the first support portions 200. It can be understood that when the first substrate 20 is driven by the first driving mechanism 500 to move along the second direction Y, the first support portions 200 can be fixed, that is, the plurality of first support portions 200 jointly provide support for the first substrate 20. This solution can not only reduce the contact area between the first support portions 200 and the first substrate 20, reduce the wear between the first substrate 20 and the first support portions 200, but also provide more stable support for the first substrate 20, thereby ensuring the stability of the transfer of the liquid 201.

[0225] In other embodiments, the first support portion 200 is roller-shaped, and the first support surface 210 is a cylindrical surface 213. The first support portions 200 are spaced apart from each other along the second direction Y. It can be understood that the intervals between adjacent first support portions 200 can be uniform intervals or non-uniform intervals. In some embodiments of the present application, the first support portions 200 are arranged at uniform intervals as an example. Each first support portion 200 is configured to be able to rotate about its own central axis as the rotation axis, that is, when the first substrate 20 is driven to move along the second direction Y, each first support portion 200 can rotate, which can further reduce the wear between the first substrate 20 and each first support portion 200, extend the service life of the first substrate 20, and ensure the stability of the transfer of the liquid 201. In other embodiments, some of the first support portions 200 can be spaced apart, and another part of the first support portions 200 can be arranged in abutment with each other. The specific arrangement can be determined according to actual conditions.

[0226] Referring to Figures 1 to 33, a second embodiment of the present invention provides a reaction device 1. The reaction device 1 comprises the liquid transfer device 10 of any of the aforementioned embodiments, a first substrate 20, and a sample carrier 30. The first support surface 210 in the reaction device 1 of this embodiment is capable of supporting the first substrate 20, enabling the stable transfer of liquid 201 carried by the first substrate 20 supported by the first support surface 210 to the reaction surface 301 of the sample carrier 30 secured by the securing structure 110. This effectively suppresses vibration of the first substrate 20, prevents scraping between the first substrate 20 and the sample carrier 30, and ensures uniform transfer of the liquid 201 to the sample carrier 30, thereby improving the stability of the transfer of the liquid 201.

[0227] Referring to Figure 26, in some embodiments, the reaction device 1 further includes a second substrate 40. It will be understood that the second substrate 40 is similar to the relevant settings of the first substrate 20. The second substrate 40 may also be a film strip made of a flexible material (such as PET, etc.). The liquid 201 carried by the second substrate 40 may be a reaction reagent or a buffer reagent, etc. The reaction reagent is used to carry out biochemical reactions and achieve imaging to complete the gene sequencing operation. The buffer reagent is used to clean the reaction reagent to prevent mixing between different reagents, which can improve the accuracy of gene sequencing. It should be noted that the liquid 201 coated on the second substrate 40 of this solution can be the same as the liquid 201 coated on the first substrate 20.

[0228] The second substrate 40 is used to replace the first substrate 20. The thickness of the second substrate 40 is greater than that of the first substrate 20. It will be appreciated that, when the gap between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 along the first direction X is constant, the thinner first substrate 20 can be replaced with the thicker second substrate 40. That is, along the first direction X, the second gap between the second substrate 40 and the sample carrier 30 is smaller than the first gap between the first substrate 20 and the sample carrier 30. Therefore, this solution allows a thinner layer of liquid 201 to be applied to the second substrate 40 to achieve liquid 201 transfer, reducing liquid 201 consumption and lowering production costs.

[0229] 27 , in some embodiments, the first substrate 20 includes a first portion 203 and a second portion 204 distributed along its length. It is understood that the first portion 203 may be connected to the second portion 204, or the first portion 203 may be disconnected from the second portion 204 (i.e., the first portion 203 and the second portion 204 are spaced apart, and the spaced apart portion may be a portion other than the first portion 203 and the second portion 204).

[0230] The thickness of the first portion 203 can be different from that of the second portion 204. In some embodiments, the thickness of the first portion 203 can be greater than the thickness of the second portion 204. In other embodiments, the thickness of the first portion 203 can be less than the thickness of the second portion 204. In the embodiment of the present application, the thickness of the first portion 203 is greater than the thickness of the second portion 204.

[0231] In this embodiment, the thickness of the first portion 203 of the first substrate 20 is greater than that of the second portion 204. This allows for a thinner layer of liquid 201 to be placed on the first portion 203 and a thicker layer of liquid 201 to be placed on the second portion 204. This allows the liquid 201 on the first and second portions 203, 204 to be transferred to the sample carrier 30. This embodiment not only reduces the consumption of liquid 201 but also adapts to the testing requirements of different liquids 201, reduces the number of sample carrier 30 replacements, and improves the efficiency of liquid 201 testing.

[0232] 28 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500 for driving the first substrate 20. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 to move in a second direction Y perpendicular to the first direction X. The explanation of the liquid exchange position is similar to that described above and will not be repeated here.

[0233] The first support surface 210 is a cylindrical surface 213, that is, the first support portion 200 is cylindrical. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z, that is, the first support portion 200 is arranged along a direction parallel to the third direction Z. The third direction Z is perpendicular to the first direction X and the second direction Y.

[0234] The first substrate 20 is arranged at least partially around the first support surface 210 along the circumference of the central axis 214 of the cylindrical surface 213, as shown in Figure 28. The first substrate 20 can only partially surround the central axis 214 of the cylindrical surface 213, or it can completely surround the central axis 214 of the cylindrical surface 213. The surrounding angle of the first substrate 20 on the first support surface 210 is greater than one hundred and eighty degrees. For example, the surrounding angle of the first substrate 20 on the first support surface 210 can be 200°, 230°, 295°, 320° or 350°, etc. The specific surrounding angle can be determined according to actual conditions. This solution can tighten the first substrate 20 by surrounding the first support surface 210, further suppressing the shaking of the first substrate 20, and ensuring the stability of the first substrate 20 during the transfer of the liquid 201.

[0235] 29 , in some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500 for driving the first substrate 20 to move. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 to move in a second direction Y perpendicular to the first direction X. Similarly, the explanation regarding the liquid exchange position is similar to that previously described and will not be repeated here.

[0236] The first support surface 210 is a cylindrical surface 213, meaning the first support portion 200 is cylindrical. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z, meaning the first support portion 200 is arranged parallel to the third direction Z. The third direction Z is perpendicular to the first direction X and the second direction Y, so the first support portion 200 is arranged perpendicular to both the first direction X and the second direction Y.

[0237] Referring to Figure 29 , the first substrate 20 is arranged at least partially around the first support surface 210 along the central axis 214 of the cylindrical surface 213. The angle a1 between the central axis 214 of the cylindrical surface 213 and the central plane 205 is 90°, meaning that the central axis 214 of the cylindrical surface 213 is perpendicular to the central plane 205. It should be noted that the central plane 205 is a plane 216 parallel to the reaction surface 301 of the sample carrier 30 when observing the first substrate 20 along the first direction X. The central plane 205 coincides with the longitudinal centerline of the first substrate 20, meaning that the arrangement direction of the first substrate 20 on the first support portion 200 coincides with the direction of movement of the first substrate 20.

[0238] In some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500 for driving the first substrate 20 to move. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 to move in a second direction Y perpendicular to the first direction X. Similarly, the explanation regarding the liquid exchange position is similar to that previously described and will not be repeated here.

[0239] The first support surface 210 is a cylindrical surface 213, meaning the outer contour of the first support portion 200 is cylindrical. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z, and the first support portion 200 is arranged along the third direction Z. The third direction Z is perpendicular to the first direction X and the second direction Y, so the arrangement direction of the first support portion 200 is perpendicular to the first direction X and the second direction Y.

[0240] Referring to Figure 30 , the first substrate 20 is arranged at least partially around the first support surface 210 along the circumference of the central axis 214 of the cylindrical surface 213. The angle a2 between the central axis 214 of the cylindrical surface 213 and the central plane 205 is less than 90 degrees, and the central plane 205 coincides with the centerline of the substrate along its own length. That is, the arrangement direction of the first substrate 20 on the first support portion 200 is inclined relative to the direction of movement of the first substrate 20. In this solution, by angling the arrangement direction of the first substrate 20 relative to the first support portion 200, the width of the liquid distribution can be reduced. Specifically, a first substrate 20 having a first width can be used to transfer liquid 201 to the reaction surface 301 of a sample carrier 30 having a second width (the second width being greater than the first width). This solution enables liquid 201 transfer and detection using a smaller first substrate 20 and a smaller amount of liquid 201, saving production costs while ensuring stable liquid 201 transfer.

[0241] Referring to Figure 31 , in some embodiments, the first supporting surface 210 of the first supporting portion 200 is a cylindrical surface 2131, meaning that the outer profile of the first supporting portion 200 is roller-shaped. The first supporting portion 200 has a first annular groove 220 arranged around its central axis. The first substrate 20 in the liquid transfer device 10 can be positioned within the first annular groove 220. The first substrate 20 can be driven to move in a direction perpendicular to the central axis 214 and the first direction X to transfer the liquid 201 on the first substrate 20 to the sample carrier 30. In this embodiment, the first supporting surface 210 of the first supporting portion 200 provides support for the first substrate 20 and the liquid 201, suppressing vibration of the first substrate 20. Furthermore, the first annular groove 220 in the first supporting portion 200 can limit the first substrate 20 positioned thereon, reducing any swaying of the first substrate 20 perpendicular to its direction of motion and further ensuring the stability of the transfer of the liquid 201.

[0242] In some embodiments, the liquid transfer device 10 includes a first substrate 20 and a second substrate 40, wherein the thickness of the first substrate 20 is greater than that of the second substrate 40. The first support surface 210 of the first support portion 200 is a cylindrical surface 2131, i.e., the outer profile of the first support portion 200 is roller-shaped. The first support portion 200 has a first annular groove 220 and a second annular groove spaced apart around a central axis 214. The first substrate 20 is disposed in the first annular groove 220, and the second substrate 40 is disposed in the second annular groove. The first substrate 20 is driven to move in a direction perpendicular to the central axis 214 and the first direction X to transfer the liquid 201 on the first substrate 20 to the sample carrier 30. In this embodiment, with a constant gap between the first support portion 200 and the sample carrier 30, the first substrate 20 and the second substrate 40 can carry layers of liquid 201 of varying thicknesses, adapting to different liquid 201 detection requirements. Furthermore, the first annular groove 220 and the second annular groove can respectively limit the displacement of the first substrate 20 and the second substrate 40, suppressing vibration of each substrate, ensuring stable transfer of the liquid 201, and preventing each substrate from scratching the sample carrier 30. It is understood that the liquid transfer device 10 may further include a third annular groove, a fourth annular groove, and the like, depending on actual circumstances.

[0243] 32 , in some embodiments, the liquid transfer device 10 includes a plurality of fixing portions 100. The specific number of fixing portions 100 may be determined based on actual conditions. For ease of understanding and description, this solution takes the provision of two fixing portions as an example. Each fixing portion 100 is used to fix a sample carrier 30, so as to simultaneously transfer liquid 201 to multiple sample carriers 30 on the plurality of fixing portions 100 using the first substrate 20. Specifically, the fixing portions 100 are arranged along a third direction Z perpendicular to the first direction X. It can be understood that the arrangement direction of the fixing portions 100 is perpendicular to the movement direction of the first substrate 20. The first supporting surface 210 at least partially faces each fixing portion 100, that is, the first supporting surface 210 may partially face each fixing portion 100, or may fully face each fixing portion 100.

[0244] The first support surface 210 is configured to support a plurality of first substrates 20 arranged along a third direction Z. The number of first substrates 20 provided may depend on the number of fixing portions 100 provided. Each first substrate 20 is used to transfer liquid 201 between each sample carrier 30 fixed to each fixing portion 100.

[0245] In this solution, the first support surface 210 can support multiple first substrates 20, and multiple fixing portions 100 can secure multiple sample carriers 30. Each first substrate 20 can transfer the liquid 201 it carries to the reaction surface 301 of the corresponding sample carrier 30 for detection and analysis. Compared to solutions that utilize a single sample carrier 30 and a single first substrate 20, this solution can simultaneously transfer liquid 201 from multiple first substrates 20 to multiple corresponding sample carriers 30, significantly improving both the efficiency of liquid 201 transfer and detection.

[0246] The third embodiment of the present invention provides an analysis device, which includes the reaction device 1 of the above embodiment and a detection device. The detection device is used to further analyze the liquid 201 on the sample carrier 30. The specific configuration can be determined according to actual conditions. In this solution, the first support surface 210 in the analysis device can support the first substrate 20, and can stably transfer the liquid 201 carried by the first substrate 20 supported by the first support surface 210 to the reaction surface 301 of the sample carrier 30 fixed by the fixing structure 110. In other words, it can effectively suppress the shaking of the first substrate 20, prevent the first substrate 20 from scratching the sample carrier 30, and transfer the liquid 201 to the sample carrier 30, thereby improving the stability of the transfer of the liquid 201. In addition, the analysis device can further detect and analyze the liquid 201 on the sample carrier 30, ensuring the accuracy of the detection results of the liquid 201.

[0247] 34 and 35 , a fourth embodiment of the present invention provides a liquid transfer method for transferring liquid 201 using the reaction device 1 of the above embodiment. The liquid transfer method includes the following steps:

[0248] S101: The fixing portion 100 is adjusted in a direction perpendicular to the first direction X until it does not overlap with the first support surface 210 in the first direction X, so as to facilitate assembly of the sample carrier 30 to the fixing structure 110 and prevent interference with the first support portion 200 during assembly. It will be appreciated that the position of the fixing portion 100 relative to the first support surface 210 can be adjusted in a second direction Y or a third direction Z perpendicular to the first direction X. Alternatively, the fixing structure 110 of the fixing portion 100 can be adjusted to not overlap with the first support surface 210 in the first direction X by a second adjustment mechanism, or by a fourth adjustment mechanism 102. The specific adjustment method may vary depending on the actual situation.

[0249] S102: Mounting the sample carrier 30 to the fixed structure 110. It is understood that the sample carrier 30 may be attached to the fixed structure 110 by suction, snap-fit, or threaded. The specific method may depend on the actual situation, as long as the sample carrier 30 and the fixed structure 110 are removably and stably connected.

[0250] S103: Adjust the fixing portion 100 in a direction perpendicular to the first direction X to a position where the first supporting surface 210 faces the reaction surface 301. It will be appreciated that the fixing portion 100 can be adjusted in a second direction Y or a third direction Z perpendicular to the first direction X to enable the first supporting surface 210 to face the reaction surface 301. When the first supporting surface 210 faces the reaction surface 301, the first substrate 20 carrying the liquid 201 can be supported on the first supporting surface 210, facilitating subsequent transfer operations of the liquid 201.

[0251] S104: Transferring the liquid 201 carried by the substrate to the sample carrier 30. After being driven, the first substrate 20 can move to the gap between the reaction surface 301 and the first support surface 210. The liquid 201 carried by the first substrate 20 contacts the reaction surface 301, and the relative movement generates shear force, thereby transferring the liquid 201. It should be noted that the fixing portion 100 that fixes the sample carrier 30 can be driven to reciprocate along the direction of movement of the first substrate 20 to uniformly transfer the liquid 201 to all locations on the reaction surface 301, ensuring detection stability.

[0252] In this solution, the sample carrier 30 can be assembled and replaced by adjusting the relative position of the fixing portion 100 and the first supporting surface 210 in a direction perpendicular to the first direction X. This eliminates the need to readjust the gap between the first supporting surface 210 and the fixing portion 100, making the operation convenient and quick, thereby improving installation efficiency. Furthermore, the support provided by the first supporting surface 210 effectively suppresses vibration of the first substrate 20, ensuring stable transfer of the liquid 201.

[0253] In some embodiments, the liquid transfer method according to the above embodiment further includes the following steps after the step of transferring the liquid 201 carried by the substrate to the sample carrier 30:

[0254] The fixing portion 100 is adjusted along a direction perpendicular to the first direction X until it does not overlap with the first supporting surface 210 along the first direction X. Similar to the adjustment method for the fixing portion 100 described above, the fixing portion 100 can be adjusted along a second direction Y or a third direction Z perpendicular to the first direction X so that the fixing portion 100 does not overlap with the first supporting surface 210 along the first direction X. Alternatively, the position of the fixing portion 100 relative to the first supporting surface 210 can be adjusted using the second adjustment mechanism or the fourth adjustment mechanism 102, depending on the actual structure.

[0255] Disassembling the sample carrier 30. Different disassembly methods can be selected according to the actual assembly and connection method between the sample carrier 30 and the fixed structure 110. In some embodiments, when the sample carrier 30 is adsorbed and connected to the fixed structure 110, the adapter structure can be used to penetrate the gap between the sample carrier 30 and the fixed structure 110 to weaken the adsorption force to achieve disassembly. In other embodiments, when the sample carrier 30 is threadedly connected to the fixed structure 110, the sample carrier 30 can be disassembled by screwing the fixing member. In other embodiments, when the sample carrier 30 is snap-connected to the fixed structure 110, the snap can be loosened to disassemble the sample carrier 30. The specific method depends on the actual structure. In addition, since the fixing portion 100 is adjusted in a direction perpendicular to the first direction X when disassembling the sample carrier 30, the gap between the first support surface 210 and the sample carrier 30 does not change. When the liquid 201 transfer operation needs to be restarted (the gap requirement is the same as the previous operation), there is no need to adjust the gap again, which makes the operation convenient.

[0256] In some embodiments, after the step of adjusting the fixing portion 100 in a direction perpendicular to the first direction X to a position where the first supporting surface 210 faces the reaction surface 301 (after step S103), and before the step of transferring the liquid 201 carried by the substrate to the sample carrier 30 (before step S104), the following steps are further included:

[0257] The distance between the reaction surface 301 and the wall of the first substrate 20 facing away from the first support surface 210 is adjusted along the first direction X. In some embodiments, the distance between the reaction surface 301 and the first substrate 20 along the first direction X can be adjusted using a first adjustment mechanism 300. Specifically, the first adjustment mechanism 300 is connected to the fixing structure 110 of the fixing portion 100, so that the reaction surface 301 of the sample carrier 30 has two different positioning positions relative to the first support surface 210. Referring to Figure 5, in the first positioning position, the sample carrier 30 can be spaced a first distance from the first support surface 210, enabling the first substrate 20 supported by the first support surface 210 to transfer the liquid 201 onto the sample carrier 30 for detection and analysis. Referring to Figure 6, in the second positioning position, the sample carrier 30 can be spaced a second distance from the first support surface 210 (the second distance is greater than the first distance), facilitating the replacement of first substrates 20 of different thicknesses or the transfer of liquids 201 of different thicknesses. In other embodiments, the first adjustment mechanism 300 can also adjust the distance between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 to accommodate first substrates 20 of different thickness specifications or to arrange liquid 201 layers of different thicknesses. In other embodiments, the distance between the reaction surface 301 and the first substrate 20 (the relative position of the first support portion 200 and the fixed portion 100) along the first direction X can be adjusted by the third adjustment mechanism 101. Specifically, the third adjustment mechanism 101 is connected to the first support portion 200. The adjustment process of the third adjustment mechanism 101 is similar to that of the first adjustment mechanism 300 and will not be repeated here.

[0258] In some embodiments, the liquid transfer device 10 further includes a first drive mechanism 500, which is used to drive the first substrate 20 to move, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30. To facilitate description and understanding of the specific movement of the first substrate 20, a second direction Y is defined. The second direction Y is any direction perpendicular to the first direction X. The description herein is based on the assumption that the second direction Y is parallel to the relative movement direction of the first substrate 20 and the sample carrier 30. For example, when the first direction X is vertical, the second direction Y is a horizontal direction perpendicular to the vertical direction; when the first direction X is horizontal, the second direction Y is a vertical direction perpendicular to the horizontal direction.

[0259] At the liquid exchange point between the first substrate 20 and the sample carrier 30, the first drive mechanism 500 is configured to drive the first substrate 20 in a second direction Y perpendicular to the first direction X. It should be noted that the location of the liquid exchange point between the first substrate 20 and the sample carrier 30 varies on different first support surfaces 210. When the first support surface 210 is a flat surface 216, the liquid exchange point is any location on the first support surface 210 that can support contact between the liquid 201 and the reaction surface 301. When the first support surface 210 is a curved surface 211, the liquid exchange point is a tangent line on the first support surface 210 that can support contact between the liquid 201 and the reaction surface 301. This embodiment uses a first support surface 210 that is a cylindrical surface 213 (part of the curved surface 211) as an example. The central axis 214 of the cylindrical surface 213 is parallel to the third direction Z, which is perpendicular to the first direction X and the second direction Y. When the first support surface 210 is a cylindrical surface 213, the liquid exchange point can be a tangent line where the liquid 201 layer contacts the sample carrier 30.

[0260] The first support surface 210 has a boundary line 215 arranged parallel to the third direction Z. The length of the boundary line 215 is the extension length of the first support surface 210 along the third direction Z. When viewed along the first direction X, the boundary line 215 is located at the end of the first support surface 210 facing the fixed structure 110 .

[0261] The liquid transfer device 10 further includes a second drive mechanism 600, which is configured to drive the fixed structure 110 to move. The second drive mechanism 600 is connected to the fixed structure 110. Specifically, the second drive mechanism 600 can be directly connected to the fixed structure 110, or can be connected to the fixed portion 100 and connected to the fixed structure 110 via the fixed portion 100.

[0262] The second drive mechanism 600 is capable of driving the fixed structure 110 to reciprocate along the second direction Y or in the opposite direction of the second direction Y to evenly apply the liquid 201 on the first substrate 20 to the reaction surface 301 of the sample carrier 30. After the fixed structure 110 secures the sample carrier 30, the boundary line 215 can pass through a predetermined area on the reaction surface 301 as viewed along the first direction X. It should be noted that the liquid 201 on the boundary line 215 can directly contact the reaction surface 301 to transfer the liquid 201 to the sample carrier 30. It should be understood that the predetermined area refers to the area on the reaction surface 301 where the liquid 201 can be detected and analyzed. The size of the predetermined area can be determined based on actual conditions. Using the boundary line 215 of the first support surface 210 to transfer the liquid 201 effectively reduces the contact area between the first substrate 20 and the sample carrier 30, preventing the first substrate 20 from scratching the sample carrier 30 and ensuring stable transfer of the liquid 201.

[0263] The steps of transferring the liquid 201 carried by the substrate to the sample carrier 30 include:

[0264] The first substrate 20 is driven to move so that the liquid 201 on the first substrate 20 can contact the reaction surface 301. Specifically, the first substrate 20 is driven to move in a direction parallel to the reaction surface 301 toward the sample carrier 30.

[0265] While the liquid 201 on the first substrate 20 contacts the reaction surface 301, the fixing unit 100 is driven to move in the opposite direction of the second direction Y, so that the liquid 201 on the first substrate 20 can be transferred to the reaction surface 301. Alternatively, while the liquid 201 on the first substrate 20 contacts the reaction surface 301, the fixing unit 100 is driven to move in the second direction Y, so that the liquid 201 on the first substrate 20 can be transferred to the reaction surface 301. The specific method may depend on the actual situation.

[0266] In some embodiments, the first support surface 210 is a cylindrical surface 2131, that is, the outer contour of the first support surface 210 is roller-shaped. The first support surface 210 arranged as a cylindrical surface 2131 can effectively tension the first substrate 20, further reduce the shaking of the first substrate 20, and improve the stability of the transfer of the liquid 201. To facilitate the description and understanding of the specific arrangement position of the cylindrical surface 2131, a third direction Z is defined. The central axis 214 of the cylindrical surface 2131 is parallel to the third direction Z, and the third direction Z is perpendicular to the first direction X. The liquid transfer device 10 also includes a first drive mechanism 500, which is used to drive the first support portion 200 to rotate about the central axis 214 of the cylindrical surface 213, thereby driving the movement of the first substrate 20. It can be understood that when the first drive mechanism 500 drives the first support portion 200 to rotate clockwise, the first substrate 20 slides in a direction from left to right. When the first driving mechanism 500 drives the first supporting portion 200 to rotate counterclockwise, the first substrate 20 slides from right to left.

[0267] To facilitate description and understanding of the specific motion trajectory of the first substrate 20, the second direction Y is defined as perpendicular to the first direction X and the third direction Z. At the liquid exchange position between the first substrate 20 and the sample carrier 30, the first support portion 200 is configured to drive the first substrate 20 to move along the second direction Y. Specifically, the first drive mechanism 500 can be used to rotate the first support portion 200. The rotating first support portion 200, leveraging tension and friction with the first substrate 20, drives the first substrate 20 in the second direction Y, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30 for testing. The cylindrical surface 2131 of the first support portion 200 ensures the stability of the first substrate 20's motion, further reducing vibration of the first substrate 20, preventing scratches on the sample carrier 30, and ensuring stable and reliable testing of the sample carrier 30.

[0268] The steps of transferring the liquid 201 carried by the substrate to the sample carrier 30 include:

[0269] The first support portion 200 is driven to rotate about the central axis 214. Specifically, the first drive mechanism 500 can be used to drive the first support portion 200 to rotate. The rotating first support portion 200, relying on tension and friction with the first substrate 20, drives the first substrate 20 in the second direction Y, thereby transferring the liquid 201 on the first substrate 20 to the sample carrier 30 for testing. The cylindrical surface 2131 of the first support portion 200 ensures the stability of the movement of the first substrate 20, further reducing the vibration amplitude of the first substrate 20, preventing scratches on the sample carrier 30, and ensuring the stability and reliability of the sample carrier 30 testing.

[0270] In some embodiments, the reaction device 1 also includes a second substrate 40. It will be understood that the second substrate 40 is similar to the relevant settings of the first substrate 20. The second substrate 40 can also be a film strip made of a flexible material (such as PET, etc.). The liquid 201 carried by the second substrate 40 can be a reaction reagent or a buffer reagent, etc. The reaction reagent is used to carry out biochemical reactions and achieve imaging to complete the gene sequencing operation. The buffer reagent is used to clean the reaction reagent to prevent mixing between different reagents, which can improve the accuracy of gene sequencing. It should be noted that the liquid 201 coated on some of the second substrates 40 of this solution is the same as the liquid 201 coated on the first substrate 20.

[0271] The second substrate 40 is used to replace the first substrate 20. The thickness of the second substrate 40 is greater than that of the first substrate 20. When the gap between the sample carrier 30 fixed to the fixed structure 110 and the first support surface 210 along the first direction X is constant, the thinner first substrate 20 can be replaced with the thicker second substrate 40. That is, along the first direction X, the second gap between the second substrate 40 and the sample carrier 30 is smaller than the first gap between the first substrate 20 and the sample carrier 30. Therefore, this solution can transfer the liquid 201 by coating a thinner layer of liquid 201 on the second substrate 40, reducing liquid 201 consumption and lowering production costs.

[0272] The liquid transfer method further comprises the following steps:

[0273] Removing the first substrate 20 : Specifically, the first substrate 20 may be moved in a direction perpendicular to the first direction X (parallel to the reaction surface 301 of the sample carrier 30 ), and removed after the first substrate 20 is spaced apart from the first support surface 210 .

[0274] The second substrate 40 is installed. Similarly, the second substrate 40 is moved in a direction perpendicular to the first direction X (parallel to the reaction surface 301 of the sample carrier 30) so that the second substrate 40 is supported on the first support surface 210. The thickness of the liquid 201 disposed on the first substrate 20 is greater than the thickness of the liquid 201 disposed on the second substrate 40. This means that the thicker second substrate 40 can replace the thinner first substrate 20. Therefore, a thinner layer of liquid 201 can be coated on the second substrate 40 to achieve liquid 201 transfer, reducing consumption of liquid 201 and lowering production costs.

[0275] In some embodiments, referring to FIG. 27 , the first substrate 20 includes a first portion 203 and a second portion 204 distributed along its length. It is understood that the first portion 203 may be connected to the second portion 204, or may be disconnected from the second portion 204 (i.e., the first portion 203 and the second portion 204 may be separated, and the separation portion may be a portion other than the first portion 203 and the second portion 204). Similarly, the first substrate 20 may further include a third portion, a fourth portion, and so on, depending on the actual situation.

[0276] The thickness of the first portion 203 can be different from that of the second portion 204. In some embodiments, the thickness of the first portion 203 can be greater than the thickness of the second portion 204. In other embodiments, the thickness of the first portion 203 can be less than the thickness of the second portion 204. In some embodiments of the present application, the thickness of the first portion 203 is greater than the thickness of the second portion 204 as an example, with reference to FIG. 27 .

[0277] In some embodiments, the liquid transfer method may include the following steps:

[0278] A first liquid 201 is arranged in the first portion 203 of the first substrate 20. It is understood that the first liquid 201 can be arranged in the first portion 203 using the liquid distributing device 700. The first liquid 201 has a first thickness. A second liquid 201 is arranged in the second portion 204 of the first substrate 20. Similarly, the second liquid 201 can be arranged in the second portion 204 using the liquid distributing device 700. The second liquid 201 has a second thickness. The second thickness of the second liquid 201 is greater than the first thickness of the first liquid 201. It should be noted that the composition of the first liquid 201 is different from that of the second liquid 201. The first liquid 201 can be a buffer reagent, and the second liquid 201 can be a reaction reagent. That is, by arranging a thicker buffer reagent in the second portion 204, the reaction reagent in the first portion 203 can be fully cleaned, thereby ensuring the accuracy of subsequent detection of subsequent reaction reagents.

[0279] In other embodiments, the liquid transfer method further comprises the following steps:

[0280] A first liquid 201 is placed on a first portion 203 of a first substrate 20. Similarly, the first liquid 201 can be placed on the first portion 203 using a liquid dispensing device 700. The first liquid 201 has a first thickness. A second liquid 201 is placed on a second portion 204 of the first substrate 20. Similarly, the second liquid 201 can be placed on the second portion 204 using a liquid dispensing device 700. The second liquid 201 has a second thickness. The second thickness is equal to the first thickness.

[0281] It should be noted that the composition of the first liquid 201 can be different from that of the second liquid 201, that is, the first liquid 201 and the second liquid 201 can be different reaction reagents. When the sample carrier 30 detects and analyzes the liquid 201, the required liquid layer thickness (the thickness of the liquid layer transferred to the reaction surface 301) for different liquids 201 compositions may be different. When a first liquid having a thickness of H1 is transferred to one portion of the reaction surface and a second liquid having a thickness of H2 (H2 thickness is less than H1 thickness) is transferred to another portion of the reaction surface, by ensuring that the thickness of the first portion 203 of the first substrate 20 is greater than that of the second portion 204 and that the thickness of the first liquid 201 and the second liquid 201 are the same, two liquid layers of different thicknesses can be simultaneously transferred to the reaction surface 301 of the sample carrier 30. This reduces reagent consumption, lowers testing costs, and eliminates the need to adjust the gap between the sample carrier 30 and the first support surface 210, enabling detection and analysis of two different reaction reagents. It is understood that the first part 203 can be separated from the second part 204, and a buffer reagent can be arranged between the first part 203 and the second part 204 to clean the reaction reagents that have completed the detection and analysis, thereby ensuring the accuracy of the detection of different reaction reagents. Similarly, the first substrate 20 can also be provided with a third part, and the third part is provided with a third liquid 201. The relevant settings of the third part can be the same as or different from those of the first part 203. The first substrate 20 can also be provided with a fourth part, and the fourth part is provided with a fourth liquid 201. The relevant settings of the fourth part can be the same as or different from those of the second part 204. By dividing the first substrate 20 into multiple parts and arranging multiple liquids 201 on different parts, the detection and cleaning needs of multiple liquids 201 can be met, thereby expanding the scope of application.

[0282] In some embodiments, the first substrate 20 includes a first portion 203 and a second portion 204 distributed along its length. Similar to the above embodiment, the first portion 203 may be connected to the second portion 204, or the first portion 203 may be disconnected from the second portion 204 (i.e., the first portion 203 and the second portion 204 are separated, and the separation portion may be other portions other than the first portion 203 and the second portion 204). Alternatively, the first substrate 20 may include a third portion, a fourth portion, and so on, depending on the actual situation. The thickness of the first portion 203 is greater than the thickness of the second portion 204. That is, when the sample carrier 30 fixed to the fixing structure 110 overlaps with the first support portion 200 along the first direction X, and the first substrate 20 is supported on the first support surface 210, the gap between the first portion 203 and the sample carrier 30 along the first direction X is smaller than the gap between the second portion 204 and the sample carrier 30.

[0283] It can be understood that in other embodiments, the first substrate 20 also includes a first part 203 and a second part 204 distributed along its own width direction. It should be noted that the liquid 201 arranged on the first part 203 and the second part 204 can be the same or different, depending on the actual situation. Similarly, the first part 203 can be connected to the second part 204, or the first part 203 can be disconnected from the second part 204. Furthermore, the first substrate 20 can also include a third part and a fourth part. The embodiment of the present application can adapt to multiple sample carriers 30 by dividing the first substrate 20 into the first part 203 and the second part 204 along its own width direction, that is, it can simultaneously transfer the liquid 201 of each part on the first substrate 20 to the reaction surfaces 301 of multiple sample carriers 30, thereby improving the transfer efficiency and detection efficiency of the liquid 201.

[0284] In some embodiments, the liquid transfer method comprises the following steps:

[0285] A first liquid 201 is placed on the first portion 203, while no liquid 201 is placed on the second portion 204. Specifically, the liquid dispensing device 700 can be used to place the first liquid 201 on the side of the first portion 203 facing the sample carrier 30, thereby transferring the first liquid 201 to the reaction surface 301 of the sample carrier 30 for detection and analysis. No liquid 201 is placed on the second portion 204 of the first substrate 20, leaving the second portion 204 empty. It will be appreciated that in this embodiment, the gap between the first portion 203 and the sample carrier 30 along the first direction X is smaller than the gap between the second portion 204 and the sample carrier 30. On the one hand, after the sample carrier 30 completes the transfer and detection of the first liquid 201 on the first portion 203, the first substrate 20 can be driven to cause the second portion 204 to overlap with the sample carrier 30 along the first direction X. This increases the gap between the first substrate 20 and the sample carrier 30, allowing the sample carrier 30 to be replaced. This method eliminates the need to readjust the gap between the new sample carrier 30 fixed to the fixing structure 110 and the first support surface 210 along the first direction X, thus eliminating the need for gap adjustment. The overall operation is convenient and quick, improving the sequencing efficiency and accuracy of the liquid 201. On the other hand, when a thinner first liquid 201 needs to be transferred to the sample carrier 30, the first liquid 201 can be transferred to the first portion 203 of the first substrate 20, reducing loss of the liquid 201 and lowering testing costs.

[0286] In other embodiments, the liquid transfer method further comprises the following steps:

[0287] The second liquid 201 is placed on the second portion 204, while no liquid 201 is placed on the first portion 203. Specifically, the liquid distributing device 700 can be used to place the second liquid 201 on the side of the first portion 203 facing the sample carrier 30, so as to transfer the second liquid 201 to the reaction surface 301 of the sample carrier 30 for detection and analysis. No liquid 201 is placed on the first portion 203, i.e., the first portion 203 is left empty. It should be noted that when a thicker second liquid 201 needs to be transferred to the sample carrier 30, since the gap between the first portion 203 and the sample carrier 30 along the first direction X is smaller than the gap between the second portion 204 and the sample carrier 30, placing the second liquid 201 in the second portion 204 can ensure the normal transfer of the second liquid 201, prevent the first substrate 20 from colliding with the sample carrier 30, and eliminate the need to adjust the gap between the sample carrier 30 and the first branch portion, thereby improving the detection efficiency and sequencing accuracy of the liquid 201.

[0288] 33 to 35 , a reaction device 1 according to an embodiment and a method for transferring a liquid 201 using the reaction device 1 are described below.

[0289] First, the specific structural arrangement of the reaction device 1 is introduced. In the reaction device 1, an unwinding mechanism 50 is provided upstream of the liquid distributing device 700. The unwinding mechanism 50 is used to drive the wound first substrate 20 to move smoothly toward the liquid distributing device 700.

[0290] A first tension swing roller 70 can be arranged between the liquid distributing device 700 and the unwinding mechanism 50. The first tension swing roller 70 can tension the first substrate 20, which can not only ensure the stability of the movement of the first substrate 20, but also enable the downstream liquid distributing device 700 to stably coat the first substrate 20 (transfer the liquid 201 to the first substrate 20).

[0291] The liquid dispensing device 700 includes a die head, which can accurately control the thickness of the liquid dispensing and can adapt to the transfer requirements of first substrates 20 and liquids 201 of various thicknesses.

[0292] Downstream of the liquid dispensing device 700, a first support portion 200 and a fixing portion 100 are arranged. The fixing portion 100 includes a fixing structure 110 that secures the sample carrier 30. The first support portion 200 is used to support the first substrate 20. The first support portion 200 has a cylindrical surface 2131, that is, the first support portion 200 has a roller body capable of supporting the first substrate 20. The cylindrical surface 2131 of the first support surface 210 effectively tensions the first substrate 20, further suppressing vibration of the first substrate 20, improving the stability of liquid 201 transfer, and preventing the first substrate 20 from scratching the sample carrier 30.

[0293] The first support portion 200 is configured to drive the first substrate 20 to rotate about the central axis 214 of the cylindrical surface 2131. In other words, the first support portion 200 can both support the first substrate 20 and drive the first substrate 20 to slide. This reduces frictional loss between the first substrate 20 and the first support portion 200, allowing the first substrate 20 to be reused multiple times while ensuring that the transfer operation of the liquid 201 can continue normally.

[0294] The liquid transfer device 10 also includes a first adjustment mechanism 300, which is connected to the fixed structure 110 and can adjust the relative position of the fixed structure 110 and the first support surface 210 along a first direction X. The first adjustment mechanism 300 enables the sample carrier 30, fixed to the fixed structure 110, to have at least two different positioning positions relative to the first support surface 210. In the first positioning position, the sample carrier 30 can be separated from the first support surface 210 by a first distance, enabling the first substrate 20 supported by the first support surface 210 to transfer the liquid 201 onto the sample carrier 30 for detection and analysis. In the second positioning position, the sample carrier 30 can be separated from the first support surface 210 by a second distance (the second distance is greater than the first distance), facilitating the replacement of first substrates 20 of different thicknesses or the transfer of liquids 201 of different thicknesses.

[0295] The liquid transfer device 10 also includes a second adjustment mechanism. This second adjustment mechanism is connected to the fixed structure 110 and can adjust the relative position of the fixed structure 110 and the first support surface 210 in a direction perpendicular to the first direction X. This facilitates the removal and replacement of the sample carrier 30 and the uniform application of the liquid 201 to the reaction surface 301 of the sample carrier 30. This solution eliminates the need to adjust the gap between the new sample carrier 30 and the first support surface 210 after replacing the sample carrier 30, making the overall operation convenient and quick.

[0296] A second tension roller 80 is disposed downstream of the fixing portion 100. This roller is used to tension the first substrate 20 after the liquid 201 has been transferred. The second tension roller 80 is connected to the winding mechanism 60 to facilitate the storage of the first substrate 20, enabling reuse of the first substrate 20 and reducing the cost of testing the liquid 201.

[0297] The reaction apparatus 1 further includes a first driving mechanism 500 for driving the first support portion 200 to rotate, thereby causing the first support portion 200 to slide the first substrate 20. The reaction apparatus 1 further includes a second driving mechanism 600, which is connected to the fixed structure 110 and is capable of driving the fixed structure 110 to reciprocate in the second direction Y or in the opposite direction of the second direction Y, thereby evenly applying the liquid 201 on the first substrate 20 to the reaction surface 301 of the sample carrier 30.

[0298] 33 , the following describes a liquid transfer method for transferring the liquid 201 using the reaction apparatus 1 described above.

[0299] S201 : Using the first adjustment mechanism 300 to adjust the relative position of the fixing structure 110 and the first support surface 210 along the first direction X, that is, to adjust the gap between the sample carrier 30 and the first support surface 210 to accommodate the transfer of the liquid 201 and the removal of the sample carrier 30 .

[0300] S202 : driving the unwinding mechanism 50 to release the wound first substrate 20 and move the first substrate 20 toward the liquid dispensing device 700 .

[0301] S203 : adjusting the first tension swing roller 70 to a predetermined angle so that the first tension swing roller 70 tensions the first substrate 20 .

[0302] S204 : adjusting the liquid dispensing device 700 , setting target parameters, and controlling the die head to coat the liquid 201 with a specified thickness onto the first substrate 20 .

[0303] S205 : Utilizing the first driving mechanism 500 to drive the first supporting portion 200 to rotate around the central axis 214 , the rotating first supporting portion 200 drives the first substrate 20 to slide.

[0304] S206 : using the second driving mechanism 600 to drive the fixed structure 110 to reciprocate along the second direction Y or in the opposite direction of the second direction Y, so as to transfer the liquid 201 on the first substrate 20 to the reaction surface 301 of the sample carrier 30 .

[0305] S207 : Using the second tension swing roller 80 to tension the first substrate 20 to which the liquid 201 has been transferred.

[0306] S208 : The first substrate 20 is wound and stored by the winding mechanism 60 , thereby completing the entire operation of detecting the liquid 201 .

[0307] S209: The second adjustment mechanism is used to adjust the relative position of the fixing structure 110 and the first supporting surface 210 in a direction perpendicular to the first direction X, thereby removing and replacing the sample carrier 30. This operation eliminates the need to adjust the gap between the new sample carrier 30 and the first supporting surface 210 after replacing the sample carrier 30. The overall operation is convenient and quick, facilitating the transfer and testing of liquids 201 of the same thickness.

[0308] The first adjustment mechanism 300 is described in detail below. In some embodiments, the guide adjustment in the first adjustment mechanism 300 may refer to a structural shape guide. Specifically, the structural shape guide may be an axial hole guide. For example, the fixed structure 110 in the fixed portion 100 is provided with an outer cylindrical surface, and the fixed portion 100 is provided with a corresponding hole. The structural shape guide may also adopt a guide form such as a square hole or a triangular hole. The first adjustment mechanism 300 can adjust the gap between the fixed structure 110 and the first support surface 210 along the first direction X by tensioning and adjusting the elastic chamber. Specifically, an elastic structure is provided in the elastic chamber, and the elastic structure may be a spring, an elastic sheet, or a compressible object. It should be noted that the deformation of the elastic chamber is very small and can be set to be within tens of times the thickness of the liquid 201 layer. The elastic force can be used to tightly connect the fixed structure 110 to the fixed portion 100 to prevent loosening.

[0309] The following describes the parameter settings of the reaction device 1 in some embodiments. The coating thickness of the liquid 201 is t, 0.02mm≤t≤0.03mm. The gap adjustment amount between the first support surface 210 and the reaction surface 301 is N, which is a fixed value after the structural adjustment, N≤n*t mm. n is determined by experiment. For example, when n is 10, N≤0.2mm. The thickness deviation of the first substrate 20 is d(p)≤0.0025mm (when the first substrate 20 has a film thickness of 100um). The cylindricity fluctuation of the cylindrical first support part 200 is d(R), d(R)≤0.0003mm. The parallelism deviation of the slide rail provided on the bracket 900 is d(H), d(H)≤0.006mm (ultra-precision grade). The gap between the first support surface 210 and the reaction surface 301 is m, m=H+NLRP. The fluctuation value of the gap m, d(m) = d(R) + d(P) + d(H), is ≤ 0.0088 mm. 0.0088 / 0.02 = 0.44, meaning the gap fluctuation value is less than half the thickness of the liquid 201 layer, preventing the first substrate 20 from scratching the sample carrier 30. Furthermore, the thickness deviation of the first substrate 20 can be within 2.5% of the thickness. For example, when a 100-micron-thick first substrate 20 is used, the thickness deviation can be 0.0025.

[0310] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0311] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features specified as "first" and "second" can explicitly or implicitly include at least one of such features. In addition, if "and / or", "and / or", or "and / or" appears in the full text, its meaning includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or solutions that meet both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention. The above are only preferred embodiments of the present invention and do not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A liquid transfer device for transferring a liquid carried by a first substrate to a sample carrier, characterized in that: The liquid transfer device comprises: a fixing portion, comprising a fixing structure, wherein the fixing structure is used to fix the sample carrier; and A first supporting portion, arranged opposite to the fixing structure along a first direction, the first supporting portion having a first supporting surface, the first supporting surface being configured to at least partially face the reaction surface of the sample carrier after the fixing structure fixes the sample carrier; The first supporting surface is used to support the first substrate, so that the liquid carried by the first substrate supported by the first supporting surface is transferred to the reaction surface of the sample carrier fixed by the fixing structure.

2. The liquid transfer device according to claim 1, characterized in that The liquid transfer device also includes a first adjustment mechanism, which is connected to the fixed structure and can adjust the relative position of the fixed structure and the first supporting surface along the first direction, so that the sample carrier fixed to the fixed structure has at least two positioning positions with different distances from the first supporting surface.

3. The liquid transfer device according to claim 1, characterized in that The liquid transfer device also includes a second adjustment mechanism, which is connected to the fixed structure and can adjust the relative position of the fixed structure and the first supporting surface along a direction perpendicular to the first direction, and the fixed structure can be adjusted by the second adjustment mechanism so that the sample carrier fixed to the fixed structure does not overlap with the first supporting surface along the first direction.

4. The liquid transfer device according to claim 3, characterized in that: The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The second adjustment mechanism is configured to adjust the relative position of the fixed structure and the first support surface along the second direction or the opposite direction of the second direction, and the fixed structure can be adjusted by the second adjustment mechanism so that the sample carrier fixed to the fixed structure does not overlap with the first support surface along the first direction.

5. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is an arc surface, the axis of the arc surface is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction.

6. The liquid transfer device according to claim 1, characterized in that The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and a central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction.

7. The liquid transfer device according to claim 6, characterized in that: The cylindrical surface is a cylindrical surface, and the first supporting portion is configured to be driven by the first substrate to rotate with the central axis of the cylindrical surface as a rotation axis.

8. The liquid transfer device according to claim 1, characterized in that The first supporting surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to the third direction, and the third direction is perpendicular to the first direction. The liquid transfer device also includes a first driving mechanism, and the first driving mechanism is used to drive the first supporting part to rotate with the central axis of the cylindrical surface as the rotation axis, thereby driving the first substrate to move; At the liquid exchange position between the first substrate and the sample carrier, the first supporting portion is configured to drive the first substrate to move along a second direction perpendicular to the first direction and the third direction.

9. The liquid transfer device according to any one of claims 5 to 8, characterized in that: The first supporting surface has a boundary line arranged parallel to the third direction, and along the first direction, the boundary line is located at an end of the first supporting surface facing the fixed structure; The liquid transfer device also includes a second driving mechanism, which is connected to the fixed structure and is configured to drive the fixed structure to reciprocate along the second direction or in the opposite direction of the second direction, so that after the fixed structure fixes the sample carrier, when observed along the first direction, the boundary line can pass through a preset area on the reaction surface.

10. The liquid transfer device according to claim 9, characterized in that After the fixing structure fixes the sample carrier and the first supporting surface supports the first substrate, the second driving mechanism is configured to drive the fixing structure to move along the second direction during the process of the first substrate transferring the carried liquid to the sample carrier; or, After the fixing structure fixes the sample carrier and the first supporting surface supports the first substrate, the second driving mechanism is configured to drive the fixing structure to move in the opposite direction along the second direction during the process of the first substrate transferring the carried liquid to the sample carrier.

11. The liquid transfer device according to claim 1, characterized in that: The fixing structure is arranged above the first supporting portion; or, The fixing structure is arranged below the first supporting portion; or, The fixing structure and the first supporting portion are arranged along a horizontal direction.

12. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device further comprises a liquid distributing device disposed upstream of the fixing portion, the liquid distributing device being used to distribute the liquid onto the first substrate; The liquid transfer device also includes a second supporting portion, which is arranged relative to the liquid distributing device at an interval, and the second supporting portion has a second supporting surface facing the liquid distributing device, and the second supporting surface is used to support the first substrate so that the liquid distributing device can distribute the liquid on the wall of the first substrate facing away from the second supporting surface.

13. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device further comprises a liquid distributing device disposed upstream of the fixing portion, the liquid distributing device being used to distribute the liquid onto the first substrate; The liquid distributing device is arranged opposite to the first supporting surface with a spacing therebetween, and the first supporting surface is used to support the first substrate, so that the liquid distributing device can distribute the liquid on a wall surface of the first substrate facing away from the first supporting surface.

14. The liquid transfer device according to claim 13, characterized in that: The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and the central axis of the cylinder is parallel to the third direction, and the third direction is perpendicular to the first direction and the second direction; The perpendicular line from the center of the liquid distributing device to the central axis is the first perpendicular line, and the perpendicular line from the center of the fixing part to the central axis is the second perpendicular line. When observed along the third direction, the angle between the first perpendicular line and the second perpendicular line is greater than or equal to ninety degrees.

15. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device further comprises a bracket, wherein the bracket is respectively connected to the first supporting part and the fixing part; The fixing portion is slidably connected to the bracket, and the fixing portion can slide until the sample carrier fixed to the fixing structure does not overlap with the first supporting surface along the first direction.

16. The liquid transfer device according to claim 15, characterized in that The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The fixing portion is configured to be able to slide relative to the bracket along the second direction or in the opposite direction of the second direction, and the fixing portion can slide until the sample carrier fixed to the fixing structure does not overlap with the first supporting surface along the first direction.

17. The liquid transfer device according to claim 15, characterized in that: The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; The first support portion is configured to be rotatable relative to the bracket with the central axis as a rotation axis.

18. The liquid transfer device according to claim 1, characterized in that: The first supporting surface is a plane, and the first supporting surface is arranged perpendicular to the first direction.

19. The liquid transfer device according to claim 18, characterized in that The first supporting surface is configured such that after the sample carrier is fixed by the fixing structure, when viewed along the first direction, the first supporting surface covers the reaction surface.

20. The liquid transfer device according to claim 18, characterized in that The first supporting surface is configured such that after the sample carrier is fixed by the fixing structure, the first supporting surface is located inside the reaction surface when viewed along the first direction; The liquid transfer device further comprises a first driving mechanism, wherein the first driving mechanism is used to drive the first substrate to move along a second direction perpendicular to the first direction; The liquid transfer device further includes a second driving mechanism, which is connected to the fixed structure and configured to drive the fixed structure to reciprocate along the second direction or in the opposite direction of the second direction.

21. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device also includes a third adjustment mechanism, which is connected to the first support part and can adjust the relative position of the first support part and the fixing part along the first direction so that the first support part has at least two positioning positions with different distances from the fixing part.

22. The liquid transfer device according to claim 1, characterized in that: The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The liquid transfer device also includes a fourth adjustment mechanism, which is connected to the first supporting portion and can adjust the relative position between the first supporting portion and the fixed portion along the second direction or in the opposite direction of the second direction, and the first supporting portion can be adjusted by the fourth adjustment structure so that the sample carrier fixed to the fixed structure does not overlap with the first supporting surface along the first direction.

23. The liquid transfer device according to claim 1, characterized in that The liquid transfer device further comprises a first driving mechanism for driving the first substrate to move At the liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move in a second direction perpendicular to the first direction; The liquid transfer device includes a plurality of first supporting parts, each of which includes a first supporting surface that is cylindrical, a central axis of each of the first supporting parts is parallel to a third direction, the third direction is respectively perpendicular to the first direction and the second direction, and an end portion of each of the first supporting surfaces close to the fixing part is arranged along the second direction.

24. The liquid transfer device according to claim 23, characterized in that Each of the first supporting portions is arranged in abutment with each other along the second direction; or, The first support portions are arranged at intervals from each other along the second direction, and each of the first support portions is configured to be rotatable with its own central axis as a rotation axis.

25. The liquid transfer device according to claim 1, characterized in that The liquid transfer device comprises a plurality of fixing parts, each of which is arranged along a third direction perpendicular to the first direction, each of which is used to fix one of the sample carriers, and the first supporting surface at least partially faces each of the fixing parts; The first supporting surface is configured to support a plurality of the first substrates arranged along the third direction, and each of the first substrates is used one by one to transfer liquid between each of the sample carriers fixed to each of the fixing parts.

26. A reaction device, characterized in that: It comprises the liquid transfer device according to any one of claims 1 to 25, the first substrate and the sample carrier.

27. The reaction device according to claim 26, characterized in that The reaction device further includes a second substrate, which is used to replace the first substrate, and the thickness of the second substrate is greater than the thickness of the first substrate.

28. The reaction device according to claim 26, characterized in that The first substrate includes a first portion and a second portion distributed along the length direction of the first substrate, and the thickness of the first portion is greater than the thickness of the second portion.

29. The reaction device according to claim 26, characterized in that The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder, and the surrounding angle of the first substrate on the first supporting surface is greater than one hundred and eighty degrees.

30. The reaction device according to claim 26, characterized in that The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at a liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder, the central axis of the cylinder is perpendicular to the central plane, and the central plane coincides with the center line of the first substrate along its own length direction.

31. The reaction device according to claim 26, characterized in that The liquid transfer device further comprises a first driving mechanism, the first driving mechanism being used to drive the first substrate to move; at the liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the The first substrate moves along a second direction perpendicular to the first direction; The first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; The first substrate is at least partially arranged around the first supporting surface along the circumference of the central axis of the cylinder, the angle between the central axis of the cylinder and the central surface is less than ninety degrees, and the central surface coincides with the center line of the substrate along its own length direction.

32. An analysis device, characterized in that: include: The reaction device according to any one of claims 26 to 31; as well as Detection device.

33. A liquid transfer method, used in the reaction device according to any one of claims 26 to 31, characterized in that: The liquid transfer method comprises the following steps: Adjusting the fixing portion along a direction perpendicular to the first direction to a position that does not overlap with the first supporting surface along the first direction; mounting the sample carrier to the fixed structure; adjusting the fixing portion along a direction perpendicular to the first direction to a position where the first supporting surface faces the reaction surface; The liquid carried by the substrate is transferred to the sample carrier.

34. The liquid transfer method according to claim 33, characterized in that: After the step of transferring the liquid carried by the substrate to the sample carrier, the following steps are also included: Adjusting the fixing portion along a direction perpendicular to the first direction to a position that does not overlap with the first supporting surface along the first direction; Disassemble the sample carrier.

35. The liquid transfer method according to claim 33, wherein: After the step of adjusting the fixing portion in a direction perpendicular to the first direction to a position where the first supporting surface faces the reaction surface and before the step of transferring the liquid carried by the substrate to the sample carrier, the following steps are also included: The distance between the reaction surface and a wall surface of the first substrate facing away from the first supporting surface is adjusted along the first direction.

36. The liquid transfer method according to claim 33, characterized in that: The liquid transfer device further comprises a first driving mechanism, which is used to drive the first substrate to move; at the liquid exchange position between the first substrate and the sample carrier, the first driving mechanism is configured to drive the first substrate to move along a second direction perpendicular to the first direction; the first supporting surface is a cylinder, and the central axis of the cylinder is parallel to a third direction, and the third direction is perpendicular to the first direction and the second direction; the first supporting surface has a boundary line arranged parallel to the third direction, and along the first direction, the boundary line is located at the end of the first supporting surface facing the fixed structure; the liquid transfer device further comprises a second driving mechanism, which is connected to the fixed structure, and the second driving mechanism is configured to be able to drive the fixed structure to reciprocate along the second direction or the reverse direction of the second direction, so that after the fixed structure fixes the sample carrier, the boundary line can pass over a preset area on the reaction surface when observed along the first direction; The step of transferring the liquid carried by the first substrate to the sample carrier comprises: driving the first substrate to move so that the liquid on the first substrate can contact the reaction surface; During the process of the liquid on the first substrate contacting the reaction surface, the fixed part is driven to move in the opposite direction along the second direction so that the liquid on the first substrate can be transferred to the reaction surface; or, during the process of the liquid on the first substrate contacting the reaction surface, the fixed part is driven to move along the second direction so that the liquid on the first substrate can be transferred to the reaction surface.

37. The liquid transfer method according to claim 36, characterized in that: The first supporting surface is a cylindrical surface, the central axis of the cylindrical surface is parallel to a third direction, and the third direction is perpendicular to the first direction. The liquid transfer device further comprises a first driving mechanism, the first driving mechanism is used to drive the first supporting part to rotate with the central axis of the cylindrical surface as a rotation axis, thereby driving the first substrate to move; at the liquid exchange position between the first substrate and the sample carrier, the first supporting part is configured to drive the first substrate to move along a second direction perpendicular to the first direction and the third direction; The step of transferring the liquid carried by the substrate to the sample carrier comprises: The first supporting portion is driven to rotate around the central axis to drive the first substrate to move.

38. The liquid transfer method according to claim 33, characterized in that: The reaction device further comprises a second substrate, the second substrate is used to replace the first substrate, and the thickness of the second substrate is greater than the thickness of the first substrate; The liquid transfer method further comprises the following steps: removing the first substrate; The second substrate is installed, wherein the thickness dimension of the liquid arranged on the first substrate is greater than the thickness dimension of the liquid arranged on the second substrate.

39. The liquid transfer method according to claim 33, characterized in that: The first substrate comprises a first portion and a second portion distributed along the length direction thereof, and the thickness of the first portion is greater than the thickness of the second portion; The liquid transfer method further comprises the following steps: disposing a first liquid on the first portion, the first liquid having a first thickness dimension; Disposing a second liquid on the second portion, the second liquid having a second thickness dimension; wherein the second thickness dimension is greater than the first thickness dimension; Or the liquid transfer method further comprises the following steps: disposing a first liquid on the first portion, the first liquid having a first thickness dimension; A second liquid is disposed on the second portion, the second liquid having a second thickness dimension; wherein the second thickness dimension is equal to the first thickness dimension.

40. The liquid transfer method according to claim 33, characterized in that: The first substrate comprises a first portion and a second portion distributed along the length direction thereof, and the thickness of the first portion is greater than the thickness of the second portion; The liquid transfer method further comprises the following steps: disposing a first liquid on the first portion and not disposing a liquid on the second portion; Or the liquid transfer method further comprises the following steps: A second liquid is disposed on the second portion, and no liquid is disposed on the first portion.

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