Composition for preventing and treating poplar catkin, preparation, preparation method and application

Abstract

The invention relates to the technical field of poplar catkin prevention and treatment, in particular to a poplar catkin prevention and treatment composition, a preparation, a preparation method and application, the composition comprises the following active ingredients: methyl jasmonate, compound sodium nitrophenolate and abscisic acid, and the mass ratio of methyl jasmonate to compound sodium nitrophenolate to abscisic acid is (50-100): 14: 1. The composition disclosed by the invention can be used for efficiently dropping poplar catkins from the female poplar plants only once, so that the aim of reducing the drifting of the poplar catkins is fulfilled.

Description

Technical Field

[0001] This invention relates to the field of poplar catkin control technology, specifically to a composition, formulation, preparation method, and application for controlling poplar catkins. Background Technology

[0002] Poplar (Populus L.) is a fast-growing tree species that can provide shade in 3-5 years, making it suitable for areas urgently needing green coverage. Poplars are highly adaptable, cold-hardy, drought-tolerant, and tolerant of poor soil, showing some tolerance to the dry climate and salinized soil of northern regions, making them suitable for urban areas with harsh environments. Poplar leaves are large, with high photosynthetic efficiency and strong carbon sequestration and oxygen release capabilities, resulting in a significant short-term improvement in air quality. Their tall canopies effectively reduce the road heat island effect, and their shading effect is particularly pronounced in summer. These characteristics make poplars a preferred tree species for urban greening projects.

[0003] Currently, common techniques for controlling poplar catkins include using plant growth regulators (such as gibberellic acid, ethephon, glyphosate, and dichlorobenzoylaminocyclopropionic acid) to interfere with catkin development. However, these methods all have certain limitations: gibberellic acid can inhibit flower bud differentiation, but its effect is unstable and requires repeated application every year, resulting in high costs; ethephon may cause non-targeted leaf drop, affecting the normal growth of trees; glyphosate is very harmful to trees, easily leading to death, and may also damage surrounding flowers, shrubs, and lawns during spraying, posing a high ecological risk; dichlorobenzoylaminocyclopropionic acid can inhibit catkins in the current year, but may cause abnormal growth and promote flower bud differentiation in the following year, leading to a rebound in the amount of catkins, requiring continuous application, and is difficult to degrade, potentially posing a long-term toxic risk to aquatic organisms. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a composition, formulation, preparation method, and application for controlling poplar catkins. The composition of this invention requires only a single application to efficiently induce catkin shedding from female poplar trees, thereby reducing catkin dispersion.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a composition for controlling poplar catkins, comprising the following active ingredients: methyl jasmonate, sodium nitrophenolate, and abscisic acid, wherein the mass ratio of methyl jasmonate, sodium nitrophenolate, and abscisic acid is (50-100):14:1.

[0007] For example, the mass ratio of methyl jasmonate, sodium nitrophenolate, and abscisic acid is any one of 50:14:1, 75:14:1, or 100:14:1, or a value between two of them. Preferably, the mass ratio of methyl jasmonate, sodium nitrophenolate, and abscisic acid is (75-100):14:1.

[0008] The active ingredient in the composition of the present invention can be purchased commercially and compounded, or a preparation containing the active ingredient can be purchased and compounded directly.

[0009] Secondly, the present invention provides a formulation for controlling poplar catkins, comprising the above-mentioned composition and pesticide-permitted excipients.

[0010] Preferably, the mass-volume concentration of the composition in the formulation is 0.0013 to 0.0072 g / mL. Exemplarily, the mass-volume concentration of the composition in the formulation is any one of 0.0013 g / mL, 0.0026 g / mL, 0.0072 g / mL, 0.0057 g / mL, 0.007 g / mL, or a value between two of these.

[0011] Preferably, the concentration of methyl jasmonate in the formulation is 0.001–0.006 g / mL, the concentration of sodium nitrophenolate is 0.00028–0.0011 g / mL, and the concentration of abscisic acid is 0.00002–0.00008 g / mL.

[0012] For example, the concentration of methyl jasmonate in the formulation is any one of 0.001 g / mL, 0.002 g / mL, 0.005 g / mL, 0.006 g / mL, or a value between two of these; the concentration of sodium nitrophenolate in the formulation is any one of 0.00028 g / mL, 0.00056 g / mL, 0.0007 g / mL, 0.00083 g / mL, 0.0011 g / mL, or a value between two of these; and the concentration of abscisic acid in the formulation is any one of 0.00002 g / mL, 0.00004 g / mL, 0.00005 g / mL, 0.00006 g / mL, 0.00008 g / mL, or a value between two of these.

[0013] Preferably, the excipients include any one or more of solvents, additives, wetting agents, dispersants, antifreeze agents, thickeners, disintegrants, fillers, and nutrients.

[0014] Preferably, the dosage form of the preparation is any one of emulsifiable concentrate, microemulsion, water emulsion, suspension concentrate, wettable powder or water-dispersible granules.

[0015] Thirdly, the present invention provides a method for preparing a composition for controlling poplar catkins, comprising mixing the active ingredients of the composition and adding excipients permitted for use in pesticides.

[0016] Fourthly, the present invention provides an application of the composition or preparation described herein in the prevention and control of poplar catkins.

[0017] Preferably, the dosage of the composition or preparation is methyl jasmonate 0.05g to 0.60g / 10cm thorax, sodium nitrophenolate 0.014g to 0.083g / 10cm thorax, and abscisic acid 0.001g to 0.006g / 10cm thorax.

[0018] For example, the application amount of methyl jasmonate is any one or a combination of 0.05 g / 10 cm, 0.10 g / 10 cm, 0.30 g / 10 cm, 0.50 g / 10 cm, and 0.60 g / 10 cm sternum diameter; the application amount of sodium nitrophenolate is any one or a combination of 0.014 g / 10 cm, 0.028 g / 10 cm, 0.056 g / 10 cm, 0.070 g / 10 cm, and 0.083 g / 10 cm sternum diameter; and the application amount of abscisic acid is any one or a combination of 0.001 g / 10 cm, 0.002 g / 10 cm, 0.004 g / 10 cm, 0.005 g / 10 cm, and 0.006 g / 10 cm sternum diameter.

[0019] Preferably, the dosage of the composition or preparation is methyl jasmonate 0.05g to 0.50g / 10cm thorax, sodium nitrophenolate 0.014g to 0.070g / 10cm thorax, and abscisic acid 0.001g to 0.005g / 10cm thorax.

[0020] Preferably, the method of preventing poplar catkins is to promote the shedding of catkins from female poplar trees and protect the branches and leaves of poplar trees.

[0021] Preferably, the composition or preparation should be used on female poplar trees before they develop fluff and unfold their leaves.

[0022] Preferably, the composition or formulation is applied once a year.

[0023] The beneficial effects of this invention are:

[0024] This invention creatively combines methyl jasmonate, sodium nitrophenolate, and abscisic acid. Abscisic acid enhances abscission layer formation, while methyl jasmonate activates defense mechanisms. Sodium nitrophenolate provides strong "logistical support" (absorption and transport) and "effect amplifier" (enhanced cellular response) for both methyl jasmonate and abscisic acid. The mechanism is unique and highly synergistic, with effects far exceeding traditional single hormones or non-synergistic combinations. Through multi-level synergistic action, it achieves highly efficient and precise regulation of the reproductive growth of female poplar trees, achieving a "one-time application, dual control" effect against cottony fluff. The functions of each active ingredient are as follows:

[0025] Methyl jasmonate (MeJA) as a core abscission inducer and flower development regulator: (1) Strongly drives abscission: As the active form of endogenous jasmonic acid in plants, MeJA is a natural and potent signal that induces abscission of organs (including abscission). It acts directly on the cells in the abscission zone at the base of the abscission stalk, strongly activating the synthesis and activity of cell wall degrading enzymes (cellulase, pectinase, etc.), and rapidly and efficiently promoting abscission formation and abscission. (2) Participates in flower development regulation: The jasmonic acid signaling pathway participates in regulating flowering time and flower organ development. Exogenous MeJA can effectively inhibit the differentiation of flower buds in female poplar trees in the following year or reduce abscission fertility by interfering with the flowering signaling network or energy allocation, providing long-term control. (3) Activates defense / stress response: MeJA is a key inducer of systemically acquired resistance (SAR), causing tree resources to tilt towards defense and maintenance growth, and indirectly inhibiting excessive reproductive growth.

[0026] Abscisic acid (ABA) is a key signaling molecule for plant organ abscission. It induces abscission layer formation by activating cell wall degrading enzymes (cellulase, polygalacturonase) in the abscission zone, promoting fluff shedding. Simultaneously, it interferes with flowering genes (FT) expression and energy allocation, inhibiting flower bud formation the following year. Furthermore, it enhances plant stress resistance, improving poplar's tolerance to drought / low temperatures, indirectly reducing the input of reproductive growth resources. The enhanced abscission layer formation by ABA and the activating defense function of MeJA complement each other, jointly inhibiting poplar reproductive growth and achieving optimal fluff shedding.

[0027] Sodium nitrophenolate (cell activator and transport enhancer): (1) Improves absorption and transport efficiency: Significantly increases the absorption rate, xylem transport efficiency and distribution uniformity of MeJA and ABA, two highly lipid-soluble molecules, in the tree, ensuring their rapid arrival at the target site (floret abscission layer, flower bud meristem). (2) Enhances cell response sensitivity: Promotes cell protoplasmic flow and metabolic activity, which may enhance the sensitivity of meristem and abscission layer cells to MeJA and ABA signals. (3) Extends the duration of action: By promoting the absorption and distribution of the agent, it indirectly extends the overall duration of action of the composition.

[0028] The application of this invention is safe and easy. A single application can effectively promote the shedding of poplar catkins, reducing many problems caused by the drifting of poplar catkins. It is suitable for industrial production and has no adverse effects on tree growth. Moreover, the active ingredient is a natural signaling molecule, which is environmentally friendly. Detailed Implementation

[0029] To enable those skilled in the art to better understand the technical solution of the invention, the invention will be further described in detail below with reference to specific embodiments.

[0030] Example 1

[0031] The active ingredients in the composition of this embodiment are methyl jasmonate, sodium nitrophenolate, and abscisic acid in a mass ratio of 50:14:1.

[0032] Formulation preparation method: Mix 0.153g of methyl jasmonate (98% by mass), 3g of sodium nitrophenolate (1.4% by mass), and 30g of abscisic acid (0.01% by mass) evenly. Mix the above components with 117ml of 75% ethanol solvent to prepare a stable microemulsion, and fill it into vials. The mass-volume concentration of the active ingredient in the formulation is 0.0013g / mL, including 0.001g / mL of methyl jasmonate, 0.00028g / mL of sodium nitrophenolate, and 0.00002g / mL of abscisic acid.

[0033] After preparing the composition, shake well. Place the composition in a vial on female poplar trees just before they develop fluff and leaves. Use one vial (50ml) for every 10cm diameter at breast height (DBH) of poplar trees (containing 0.05g methyl jasmonate / 10cm DBH, 0.014g sodium nitrophenolate / 10cm DBH, and 0.001g abscisic acid / 10cm DBH). Use only once a year. The application was conducted on March 27, 2025. Before application, select female poplar trees with a DBH of approximately 30cm, mark the branches, and count the number of fluffs on the female trees before application. A blank control group was injected with an equal volume of water. On April 26, count the remaining fluff on the marked branches after application. Use three trees in each group (treatment group and control group), marking one branch on each tree for counting.

[0034] Thirty days after the medication was applied, the number of remaining poplar catkins on the marked branches of the medication group and the control group was statistically recorded.

[0035] The experimental data and comparison results are as follows:

[0036] Table 1. Remaining amount of poplar catkins in the drug-treated and control groups

[0037]

[0038]

[0039] As shown in Table 1, after 30 days of treatment, 65% of the poplar catkins in the treated group fell off, while the number of poplar catkins in the control group remained basically unchanged. Furthermore, the subsequent leaf growth of the poplar trees was normal, and no leaf drop occurred. This indicates that the composition of this embodiment can significantly promote the shedding of poplar catkins from female poplar trees, reduce catkin drift, and has no effect on the growth of poplar trees.

[0040] Example 2

[0041] The active ingredients in the composition of this embodiment are methyl jasmonate, sodium nitrophenolate, and abscisic acid in a mass ratio of 50:14:1.

[0042] Preparation method: Mix 0.306g of methyl jasmonate (98% by mass), 6g of sodium nitrophenolate (1.4% by mass), and 60g of abscisic acid (0.01% by mass) evenly. Mix the above components with 84ml of 75% ethanol solvent to prepare a stable microemulsion, and fill it into vials. The mass-volume concentration of the active ingredient in the formulation is 0.0026g / mL, including 0.002g / mL of methyl jasmonate, 0.00056g / mL of sodium nitrophenolate, and 0.00004g / mL of abscisic acid.

[0043] After preparing the composition, shake well. Place the composition in a vial on female poplar trees just before they develop fluff and leaves. Use one vial (50ml) for every 10cm diameter at breast height (DBH) of poplar trees (containing 0.10g / 10cm DBH of methyl jasmonate, 0.028g / 10cm DBH of sodium nitrophenolate, and 0.002g / 10cm DBH of abscisic acid). Use only once a year. The application was conducted on March 27, 2025. Before application, select female poplar trees with a DBH of approximately 30cm, mark the branches, and count the number of fluffs on the female trees before application. A blank control group was injected with an equal volume of water. On April 26, count the remaining fluff on the marked branches after application. Use three trees in each group (treatment group and control group), marking one branch on each tree for counting.

[0044] Thirty days after the medication was applied, the number of remaining poplar catkins on the marked branches of the medication group and the control group was statistically recorded.

[0045] The experimental data and comparison results are as follows:

[0046] Table 2. Remaining Poplar Catkin Quantity in Drug-treated and Control Groups

[0047]

[0048]

[0049] As shown in Table 2, after 30 days of treatment, 70% of the poplar catkins in the treated group fell off, while the number of poplar catkins in the control group remained basically unchanged. Furthermore, the subsequent leaf growth of the poplar trees was normal, and no leaf drop occurred. This indicates that the composition of this embodiment can significantly promote the shedding of poplar catkins from female poplar trees, reduce catkin drift, and has no effect on the growth of poplar trees.

[0050] Example 3

[0051] The active ingredients in the composition of this embodiment are methyl jasmonate, sodium nitrophenolate, and abscisic acid in a mass ratio of 75:14:1.

[0052] Preparation method: Mix 0.918g of methyl jasmonate (98% by mass), 12g of sodium nitrophenolate (1.4% by mass), and 120g of abscisic acid (0.01% by mass) evenly. Mix the above components with 18ml of 75% ethanol solvent to prepare a stable microemulsion, which is then filled into vials. The mass-volume concentration of the active ingredient in the formulation is 0.0072g / mL, including 0.006g / mL of methyl jasmonate, 0.0011g / mL of sodium nitrophenolate, and 0.00008g / mL of abscisic acid.

[0053] After preparing the composition, shake well. Place the composition in a vial on female poplar trees just before they develop fluff and leaves. Use one vial (50ml) for every 10cm diameter at breast height (DBH) of poplar trees (containing 0.30g / 10cm DBH of methyl jasmonate, 0.056g / 10cm DBH of sodium nitrophenolate, and 0.004g / 10cm DBH of abscisic acid). Use only once a year. The application was conducted on March 28, 2025. Before application, select female poplar trees with a DBH of approximately 30cm, mark the branches, and count the number of fluffs on the female trees before application. A blank control group was injected with an equal volume of water. On April 27, count the remaining fluff on the marked branches after application. Use three trees in each group (treatment group and control group), marking one branch on each tree for counting.

[0054] Thirty days after the medication was applied, the number of remaining poplar catkins on the marked branches of the medication group and the control group was statistically recorded.

[0055] The experimental data and comparison results are as follows:

[0056] Table 3. Remaining amount of poplar catkins in the drug-treated and control groups

[0057]

[0058]

[0059] As shown in Table 3, after 30 days of treatment, 74% of the poplar catkins in the treated group fell off, while the number of poplar catkins in the control group remained basically unchanged. Furthermore, the subsequent leaf growth of the poplar trees was normal, and no leaf drop occurred. This indicates that the composition of this embodiment can significantly promote the shedding of poplar catkins from female poplar trees, reduce catkin drift, and has no effect on the growth of poplar trees.

[0060] Example 4

[0061] The active ingredients in the composition of this embodiment are methyl jasmonate, sodium nitrophenolate, and abscisic acid in a mass ratio of 100:14:1.

[0062] Preparation method: Mix 1.53g of methyl jasmonate (98% by mass), 15g of sodium nitrophenolate (1.4% by mass), and 150g of abscisic acid (0.01% by mass) evenly. Mix the above components with 135ml of 75% ethanol solvent to prepare a stable microemulsion, and fill it into vials. The mass-volume concentration of the active ingredient in the formulation is 0.0057g / mL, including 0.005g / mL of methyl jasmonate, 0.0007g / mL of sodium nitrophenolate, and 0.00005g / mL of abscisic acid.

[0063] After preparing the composition, shake well. Place the mixture in a vial on female poplar trees just before they develop fluff and leaves. Use one vial (100ml) for every 10cm diameter at breast height (DBH) of poplar trees (containing 0.5g / 10cm DBH of methyl jasmonate, 0.07g / 10cm DBH of sodium nitrophenolate, and 0.005g / 10cm DBH of abscisic acid). Use only once a year. The application was conducted on March 29, 2025. Before application, select female poplar trees with a DBH of approximately 30cm, mark the branches, and count the number of fluffs on the female trees before application. A blank control group was injected with an equal volume of water. On April 28, count the remaining fluff on the marked branches after application. Use three trees in each group (treatment group and control group), marking one branch on each tree for counting.

[0064] Thirty days after the medication was applied, the number of remaining poplar catkins on the marked branches of the medication group and the control group was statistically recorded.

[0065] The experimental data and comparison results are as follows:

[0066] Table 4. Remaining Poplar Catkin Quantity in Drug-Treatment and Control Group

[0067]

[0068]

[0069] As shown in Table 4, after 30 days of treatment, 86% of the poplar catkins in the treated group fell off, while the number of poplar catkins in the control group remained basically unchanged. Furthermore, the subsequent leaf growth of the poplar trees was normal, and no leaf drop occurred. This indicates that the composition of this embodiment can significantly promote the shedding of poplar catkins from female poplar trees, reduce catkin drift, and has no effect on the growth of poplar trees.

[0070] Example 5

[0071] The active ingredients in the composition of this embodiment are methyl jasmonate, sodium nitrophenolate, and abscisic acid in a mass ratio of 100:14:1.

[0072] Preparation method: Mix 1.836g of methyl jasmonate (98% by mass), 18g of sodium nitrophenolate (1.4% by mass), and 180g of abscisic acid (0.01% by mass) evenly. Mix the above components with 102ml of 75% ethanol solvent to prepare a stable microemulsion, which is then filled into vials. The mass-volume concentration of the active ingredient in the formulation is 0.007g / mL, including 0.006g / mL of methyl jasmonate, 0.00083g / mL of sodium nitrophenolate, and 0.00006g / mL of abscisic acid.

[0073] After preparing the composition, shake well. Place the composition in a vial on female poplar trees just before they develop fluff and leaves. Use one vial (100ml) for every 10cm diameter at breast height (DBH) of poplar trees (containing methyl jasmonate 0.6g / 10cm DBH, sodium nitrophenolate 0.083g / 10cm DBH, and abscisic acid 0.006g / 10cm DBH). Use only once a year. The application was conducted on March 29, 2025. Before application, select female poplar trees with a DBH of approximately 30cm, mark the branches, and count the number of fluffs on the female trees before application. A blank control group was injected with an equal volume of water. On April 28, count the remaining fluff on the marked branches after application. Use three trees in each group (treatment group and control group), marking one branch on each tree for counting.

[0074] Thirty days after the medication was applied, the number of remaining poplar catkins on the marked branches of the medication group and the control group was statistically recorded.

[0075] The experimental data and comparison results are as follows:

[0076] Table 5. Remaining Poplar Catkin Quantity in Drug-treated and Control Groups

[0077]

[0078]

[0079] Table 5 shows that after 30 days of treatment, 88.9% of the poplar catkins in the treated group fell off, a slight improvement compared to Example 4. However, the treated group experienced significant leaf drop, while the control group maintained a relatively stable number of catkins and did not experience any leaf drop. This indicates that excessive use can lead to premature leaf drop in poplar trees, affecting the landscape effect.

[0080] Comparative Example 1

[0081] Same as Example 4, except that the composition does not contain methyl jasmonate.

[0082] The results are shown in Table 6.

[0083] Table 6. Remaining Poplar Catkin Quantity in Drug-Treatment and Control Groups

[0084]

[0085] As shown in Table 6, if methyl jasmonate is not present in the composition, the poplar catkins in the treated group will not fall off at all. Sodium nitrophenolate and abscisic acid alone cannot achieve the effect of poplar catkin removal.

[0086] Comparative Example 2

[0087] Same as Example 4, except that sodium nitrophenolate is not present in the composition.

[0088] The results of spraying the composition are shown in Table 7.

[0089] Table 7. Remaining Poplar Catkin Quantity in Drug-Treatment and Control Group

[0090]

[0091] As shown in Table 7, although the drug treatment group can promote the shedding of poplar catkins to a certain extent without sodium nitrophenolate in the composition, with 35.8% of the catkins falling, it is still quite different from Example 4. Methyl jasmonate and abscisic acid alone cannot effectively promote the shedding of poplar catkins.

[0092] Comparative Example 3

[0093] Same as Example 4, except that: the composition does not contain abscisic acid.

[0094] The results of spraying the composition are shown in Table 8.

[0095] Table 8. Remaining Poplar Catkin Quantity in Drug-Treatment and Control Groups

[0096]

[0097] As shown in Table 8, if the composition does not contain abscisic acid, although the drug treatment group can promote the shedding of poplar catkins to a certain extent, with 46.1% of the catkins falling, it is still quite different from Example 4. Methyl jasmonate and sodium nitrophenolate alone cannot effectively promote the shedding of poplar catkins.

[0098] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be considered as limitations on the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. For those skilled in the art, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A composition for controlling poplar catkins, characterized in that, It includes the following active ingredients: methyl jasmonate, sodium nitrophenolate, and abscisic acid, wherein the mass ratio of methyl jasmonate, sodium nitrophenolate, and abscisic acid is (50-100):14:

1.

2. A preparation for controlling poplar catkins, characterized in that, Includes the composition of claim 1 and pesticide-permitted excipients.

3. The formulation according to claim 2, characterized in that, The mass-volume concentration of the composition in the formulation is 0.0013 to 0.0072 g / mL.

4. The formulation according to claim 2 or 3, characterized in that, The concentration of methyl jasmonate in the formulation is 0.001–0.006 g / mL, the concentration of sodium nitrophenolate is 0.00028–0.0011 g / mL, and the concentration of abscisic acid is 0.00002–0.00008 g / mL.

5. A method for preparing a composition for controlling poplar catkins, characterized in that, This includes mixing the active ingredients of the composition according to claim 1 and then adding excipients permitted for use in pesticides.

6. The use of the composition according to claim 1 or the formulation according to any one of claims 2 to 4 in the prevention and control of poplar catkins.

7. The application according to claim 6, characterized in that, The dosage of the composition or preparation is methyl jasmonate 0.05g to 0.60g / 10cm thorax, sodium nitrophenolate 0.014g to 0.083g / 10cm thorax, and abscisic acid 0.001g to 0.006g / 10cm thorax.

8. The application according to claim 6, characterized in that, The method for preventing and controlling poplar catkins involves promoting the shedding of catkins from female poplar trees while protecting the branches and leaves of the poplar trees.

9. The application according to claim 6, characterized in that, The composition or preparation must be used on female poplar trees before they develop fluff and unfold their leaves.

10. The application according to claim 6, characterized in that, The composition or preparation is to be applied once a year.

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