Drought resistance inducer

Abstract

The application discloses a drought resistance inducer, which mainly utilizes potassium persulfate to be sprayed on plant leaves 3-7 days before the plant faces drought stress, simulates early drought stress signals by inducing active oxygen production of potassium persulfate, triggers the antioxidant defense system in the plant body, simultaneously induces proline biosynthesis, and activates the osmotic regulation mechanism. On this basis, the application also synchronously improves the adaptability of the plant to water stress in a drought environment by corresponding proportioning of metabolic substances for regulating water stress response, utilizes metabolic support components in the sprayed inducer to form a synergistic network of osmotic regulation and energy supply, so that the application can promote the synthesis of proline, improve the osmotic regulation capacity of wheat, and realize synergistic enhancement of drought resistance from signal perception to physiological execution through multi-level linkage of potassium persulfate signal early warning, water response synergy and metabolic network support physiological function maintenance.

Description

Technical Field

[0001] This application belongs to the field of plant growth regulator technology, and in particular relates to a drought resistance inducer. Background Technology

[0002] Drought is one of the major abiotic stresses affecting crop growth. Under drought conditions, soil moisture content decreases, significantly reducing the water that crop roots can absorb. This leads to an imbalance between root water absorption and transpiration, causing cell dehydration and disrupting metabolic mechanisms. Plants are unable to transport and utilize water and nutrients normally, which not only severely hinders crop growth and development but also weakens their resistance, making them more susceptible to pests and diseases, ultimately resulting in reduced yields or even crop failure.

[0003] Wheat, as a globally important food crop, exhibits phased water requirements during its growth and development, and its production is particularly significantly affected by drought stress. When wheat is subjected to drought stress, its photosynthetic capacity decreases significantly, root activity declines, and the absorption efficiency of key nutrients such as nitrogen, phosphorus, and potassium is drastically reduced. Overall, the plant exhibits leaf curling, wilting, darkening or yellowing, a decrease in tillering number, a lower ear formation rate, a shortened grain-filling period, and insufficient grain filling, ultimately leading to smaller ears and shriveled grains, severely impacting wheat yield and quality. Especially during critical growth stages such as the jointing and booting stages, prolonged drought can even cause large-scale plant death, resulting in devastating yield reductions.

[0004] Currently, measures to address drought stress mainly fall into three categories: variety improvement, field management, and post-disaster remediation. However, these measures have certain limitations and cannot fundamentally solve the problem. While variety improvement can cultivate some drought-resistant wheat varieties, its long breeding cycle and susceptibility to environmental influences make it difficult to promote and apply in the short term. Field management measures often fail to function promptly in the face of sudden drought disasters. Existing post-disaster remediation measures rely on the application of plant growth regulators or nutrient supplements after drought occurs. Although these can alleviate plant damage to some extent, their response to stress is delayed and often ineffective in counteracting the yield reduction caused by drought. Furthermore, existing products for drought stress regulation are usually costly, have complex preparation processes, and present certain technical barriers to use, making them difficult to popularize in large-scale agricultural production.

[0005] Therefore, there is an urgent need for a technical solution that can not only preventively treat wheat before drought stress occurs to enhance its drought resistance, but also has the advantages of simple preparation, low cost and convenient use, in order to meet the needs of large-scale agricultural production, improve wheat's adaptability to drought stress and reduce the impact of drought environment on wheat yield and quality. Summary of the Invention

[0006] The problem this application aims to solve is that existing technologies cannot respond in a timely manner to the irreversible effects of drought stress on wheat plants, and reduce the impact of drought on wheat yield and quality.

[0007] To solve the above-mentioned technical problems, the technical solution adopted in this application is as follows: a drought-resistant inducer, comprising: a 50 μM potassium persulfate solution, wherein the solvent of the potassium persulfate solution is a sucrose solution with a mass concentration of 5 g / L; the drought-resistant inducer is sprayed on the leaves of the plant 3-7 days before the plant faces drought stress; the amount of potassium persulfate solution sprayed is such that each leaf of the plant forms a droplet of water that flows down; direct sunlight on the seedlings is avoided during spraying.

[0008] Optionally, the drought-inducing agent as described above may further contain any of the following components or combinations thereof dissolved in it: potassium dihydrogen phosphate and urea; in a 50 μM potassium persulfate solution, the concentration of potassium dihydrogen phosphate is 5 g / L and the concentration of urea is 5 g / L.

[0009] Optionally, the drought-inducing agent as described above may further contain: water-soluble silicon fertilizer; the concentration of the water-soluble silicon fertilizer in a 50 μM potassium persulfate solution is 2 ml / L.

[0010] Optionally, the drought-inducing agent as described above may further contain dissolved earthworm enzymes; the concentration of earthworm enzymes in a 50 μM potassium persulfate solution is 3 ml / L.

[0011] Optionally, the drought-inducing agent as described above, wherein each liter of the drought-inducing agent comprises: 50 μmol of potassium persulfate, a preset dose of a water-coordinating component and a metabolic support component; wherein the water-coordinating component comprises: a concentration of 2 ml of water-soluble silicon fertilizer and / or 5 g of potassium dihydrogen phosphate; and the metabolic support component comprises: 5 g of urea and / or 5 g of sucrose.

[0012] Optionally, the drought-inducing agent as described above, wherein each liter of the drought-inducing agent comprises: 50 μmol of potassium persulfate, 5 g of sucrose, 5 g of potassium dihydrogen phosphate, 5 g of urea and 2 ml of water-soluble silicon fertilizer.

[0013] Optionally, the drought-inducing agent as described above may further include 3 ml of earthworm enzyme per liter of the drought-inducing agent.

[0014] Optionally, the drought-inducing agent as described above is sprayed onto the plant leaves at the three-leaf stage or 3-7 days before the plant faces drought stress during the seedling stage; the spraying amount is such that each leaf of the plant forms a droplet of water that flows down; direct sunlight on the seedlings should be avoided during spraying.

[0015] Beneficial effects:

[0016] The drought-inducing agent provided in this application mainly utilizes potassium persulfate, sprayed onto the leaves of plants 3-7 days before they face drought stress. Potassium persulfate induces reactive oxygen species (ROS) to generate signals simulating early drought stress, triggering the plant's antioxidant defense system and simultaneously inducing proline biosynthesis, activating osmotic regulation mechanisms. Building upon this, this application also simultaneously enhances the plant's adaptability to water stress under drought conditions through a pre-set dose of water-coordinating components. The metabolic support components in the sprayed inducer form a synergistic network of osmotic regulation and energy supply. Therefore, this application can promote proline synthesis and improve the osmotic regulation capacity of wheat through a multi-level linkage of potassium persulfate signal warning → water response coordination → metabolic network support, achieving a synergistic enhancement of drought resistance from signal perception to physiological execution. Attached Figure Description

[0017] The embodiments of this application will be described in further detail below with reference to the accompanying drawings:

[0018] Figure 1 A comparative diagram of wheat growth phenotypes after spraying with different drought-inducing agents;

[0019] Figure 2 A comparative graph showing wheat growth phenotypes and drought resistance after spraying with different drought-inducing agents;

[0020] Figure 3 A comparative graph showing the effects of different drought-inducing agents on the photosynthetic rate and stomatal conductance of wheat leaves;

[0021] Figure 4 A comparative graph showing the effects of different drought-inducing agents on the photosynthetic rate and stomatal conductance of wheat leaves under drought stress;

[0022] Figure 5 A comparative graph showing the effects of different drought-inducing agents on the MDA content of wheat leaves;

[0023] Figure 6 A comparative graph showing the effects of different drought-inducing agents on the MDA content of wheat leaves under drought stress;

[0024] Figure 7 A comparative graph showing the effects of different drought-inducing agents on wheat proline content under drought stress. Detailed Implementation

[0025] The existing drought stress regulation mechanisms face the following problems: (1) the plant selection cycle is too long and it is difficult to ensure the economic benefits of plants selected for drought resistance under normal conditions; (2) spraying measures after drought can only alleviate plant damage but cannot effectively reduce the negative impact of drought on plant yield; (3) existing products that provide regulation effects against drought stress are expensive and have complicated preparation processes.

[0026] This application provides a drought-inducing agent, the main active ingredient of which is a 50 μM potassium persulfate solution. This application describes spraying the 50 μM potassium persulfate solution onto the plant leaves 3-7 days before the plant faces drought stress, choosing a time in the early morning or evening when there is no direct sunlight. This causes each leaf to form droplets that flow down, thus inducing the production of reactive oxygen species (ROS) within the plant cells, mimicking the stress signals of the early stages of drought. This triggers the plant's antioxidant defense system, enhancing its adaptability to drought stress conditions. Simultaneously, it induces proline biosynthesis and activates osmotic regulation mechanisms to reduce the negative impact of the drought environment on plant growth in the following days.

[0027] Potassium persulfate ( As a strong oxidizing agent, it is not typically used in agricultural or plant physiological research, and research on its use as a plant growth regulator or fertilizer is even rarer. Currently, research on its effects on plants under arid conditions is also very limited.

[0028] While existing research on potassium persulfate as a plant growth regulator has shown its ability to overcome hypoxic metabolic disorders and membrane system instability under waterlogging stress, its effects on plant resistance under other stress conditions have not been studied. This application innovatively utilizes potassium persulfate as an early warning medium for drought stress. By stimulating redox reactions within the plant, it influences the intracellular antioxidant system, promoting the activity of substances such as superoxide dismutase and catalase. This induces higher antioxidant capacity in plants before drought stress occurs, and subsequently mitigates stomatal conductance issues under drought stress. Simultaneously, it induces proline biosynthesis and activates osmotic regulation mechanisms, thereby enhancing the plant's drought resistance and reducing the negative impact on dry matter accumulation and crop yield reduction caused by drought.

[0029] In a preferred embodiment, this application further modifies the core component of potassium persulfate by replacing the water-based potassium persulfate solution with a 5 g / L sucrose solution. This utilizes the synergistic effect of sucrose and potassium persulfate as core components, leveraging the reactive oxygen species (ROS) induced by potassium persulfate as a stress signal to preemptively activate plant stress-resistance gene expression and defense metabolic pathways. Sucrose then provides dual support in this process. The mixed 50 μM potassium persulfate and 5 g / L sucrose solution is used as a drought inducer. Three days before the plants face drought stress, it is sprayed onto the plant leaves in the early morning or late afternoon when there is no direct sunlight, causing each leaf to form droplets that flow down. On the fifth day of drought stress, a significant reduction in membrane lipid peroxidation levels and a marked improvement in stomatal condition can be detected in plants sprayed with this drought inducer. This demonstrates that the drought-inducing agent composed of 50 μM potassium persulfate and 5 g / L sucrose solution can achieve a significant mitigation effect on drought stress.

[0030] Therefore, this embodiment achieves signal-energy coupling within the plant through potassium persulfate and sucrose. Under this synergistic effect, sucrose provides additional dual support for the action of potassium persulfate:

[0031] (1) Energy supply: As a substrate for respiratory metabolism such as glycolysis, it rapidly generates ATP, which provides the necessary energy for defense responses triggered by ROS signals, such as the synthesis of antioxidant enzymes, and avoids signal transduction failure due to energy deficiency;

[0032] (2) Carbon skeleton supply: provides carbon skeleton for the synthesis of downstream osmotic regulators, such as proline.

[0033] Effects: It avoids the disconnect between ROS signal transduction and downstream defense metabolic response caused by energy deficiency, ensures that stress warning signals can be effectively triggered and complete the full physiological cascade reaction, and lays the energy metabolism foundation for the anti-stress response.

[0034] In a more preferred embodiment, the drought-inducing agent of this application further dissolves 5 g / L potassium dihydrogen phosphate or 5 g / L urea in the aforementioned 5 g / L sucrose and 50 μM potassium persulfate solution. The added potassium dihydrogen phosphate or urea in this embodiment can introduce key nutrients such as nitrogen (N), phosphorus (P), and potassium (K), transforming the initial signal-energy coupling into efficient substance synthesis. The above mixture was sprayed onto the plant leaves under conditions of no intense sunlight for 5 days before drought stress, resulting in water droplets forming on each leaf after spraying. On the 7th day of drought stress, a significant improvement in the phenotype of plants sprayed with this drought-inducing agent under drought stress was observed, with increased synthesis of osmotic regulators. Therefore, it can be confirmed that the solutions of 50 μM potassium persulfate, 5 g / L sucrose and 5 g / L potassium dihydrogen phosphate, or the solutions of 50 μM potassium persulfate, 5 g / L sucrose and 5 g / L urea, can effectively enable crops to acquire resistance to drought stress.

[0035] Therefore, this embodiment, through potassium persulfate + sucrose + potassium dihydrogen phosphate + urea, further constructs a secondary synergistic mechanism that can promote and amplify the metabolic network by synergistically combining potassium dihydrogen phosphate and urea, building upon the coupling of type and energy provided in the previous embodiment. Under this mechanism:

[0036] (1) Potassium dihydrogen phosphate: provided It is an activator of various stress-related enzymes and acts as an osmotic regulator ion; providing It is a key component in the synthesis of ATP, nucleic acids, and phospholipids, ensuring energy conversion and membrane structure stability;

[0037] (2) Urea: provides a nitrogen source and is an essential raw material for the synthesis of key stress-resistant substances such as osmotic protective proteins and proline.

[0038] In this embodiment: potassium persulfate signaling initiates the defense program, while sucrose and potassium dihydrogen phosphate provide energy (ATP) and driving force (…). Urea and sucrose provide the raw materials for synthesis (N, C), and potassium ions in potassium dihydrogen phosphate ( ) and phosphate ( These four components perform the dual functions of membrane potential stabilization and ATP synthesis. Together, they drive the rapid and large-scale synthesis of osmotic regulatory substances, transforming warning signals into physiological resilience. This converts the primary energy metabolism advantage into a highly efficient capacity for synthesizing resilience substances, forming a self-circulating network of osmotic regulatory substance synthesis and energy supply.

[0039] Furthermore, this application can further add water-soluble silicon fertilizer at a concentration of 2 ml / L to the drought-inducing agent provided in the previous embodiment. The water-soluble silicon fertilizer added in this embodiment, based on the previous embodiment, can further enhance water regulation through physical integration and physiological synergy. Building upon the powerful osmotic regulation and energy metabolism network constructed by the aforementioned secondary synergy, the introduction of water-soluble silicon fertilizer achieves a mechanistic leap from simple physiological water retention to a synergistic effect of physical water blocking and physiological water retention. The above mixture was sprayed onto the plant leaves under conditions of no intense sunlight during the first 7 days of drought stress, resulting in water droplets forming on each leaf after spraying. On the 7th day of drought stress, it was significantly observed that the plants sprayed with this drought-inducing agent showed a significant improvement in their phenotype under drought stress, with a significantly increased net photosynthetic rate and increased leaf water potential. Therefore, it can be confirmed that the solution of 50 μM potassium persulfate, 5 g / L sucrose and 5 g / L potassium dihydrogen phosphate, or the solution of 50 μM potassium persulfate, 5 g / L sucrose and 5 g / L urea combined with 3 ml / L earthworm enzyme can effectively enable crops to obtain resistance to drought stress.

[0040] In this embodiment, a bidirectional positive feedback can be generated between the physical inhibition mechanism and the existing physiological metabolic system, thereby:

[0041] (1) Enhanced physiological regulation effectiveness: Reduced water transpiration loss allows osmotic regulators such as proline and betaine, which are synthesized by urea, sucrose and potassium dihydrogen phosphate, to maintain cell turgor pressure and leaf water potential more persistently and effectively, thus significantly improving water use efficiency.

[0042] (2) Optimize early warning capabilities and improve enzyme system function: By maintaining a more stable intracellular water status and osmotic environment through physical means, it provides the optimal working environment for high-energy-consuming enzyme systems activated by potassium persulfate early warning signals, such as SOD, POD, and CAT, thereby maximizing their catalytic efficiency.

[0043] In other words, the physical mechanism provided by the silicon fertilizer added in this application can be combined with the physiological tolerance provided by the metabolic network, thus forming a synergistic enhancement of drought resistance through the dual pathways of reducing water loss and enhancing water retention.

[0044] In the most preferred embodiment, considering the effect of earthworm enzyme in enhancing the plant's antioxidant system and reducing cellular oxidative damage, this application may further add earthworm enzyme at a concentration of 3 ml / L per liter of the above-mentioned drought-inducing agent to further achieve a better drought-inducing effect in conjunction with potassium persulfate.

[0045] The verification experiment of this embodiment was conducted in a climate chamber. Small potted plants were used. After soaking and disinfecting, the seeds of Yangmai 16 were placed on damp gauze and incubated in the dark at 25°C for 24 hours until the wheat sprouted. Then, 11.4g of urea and 5.2g of potassium dihydrogen phosphate were added to 10L of water and stirred until completely dissolved to prepare fertilizer solution. 600g of soil was added to each pot, and 200ml of fertilizer solution was used to compact the soil. The sprouted wheat seeds were then evenly placed with the grooves facing down, and finally covered with 50g of soil to complete the preparation for the experiment. Before drought stress, different drought-inducing agents were applied to each group of seeds, for example, spraying C (control + drought stress); T1 (spraying T1 agent + drought stress); N1 (spraying N1 agent + drought stress); and N2 (spraying N2 agent + drought stress) to verify the drought-inducing effect of T1 in this embodiment. The drought-inducing agent formulations labeled T1, N1, and N2 used in the experiment were set as follows:

[0046] Table 1. Preparation of drought resistance inducers

[0047]

[0048] Drought-resistant inducer spraying can be carried out during the seedling stage or concentrated at the three-leaf-one-heart stage. Spraying should be done twice, at 8:00 AM and 4:00 PM, to avoid direct sunlight. Each sample was sprayed onto the leaves of each plant, forming water droplets that flowed down. Then, drought stress was applied during the four-leaf-one-heart stage (3-7 days after spraying) by controlling water supply. The drought stress lasted for 7 days. Samples were taken from the terminal leaves on days 1, 2, and 3 after drought inducer spraying, and on days 1, 3, and 5 after the onset of drought stress, to determine the effects of the inducer on wheat growth phenotype, photosynthesis, and the content of MDA and proline under drought stress.

[0049] refer to Figure 1 The different names in the table represent the following meanings: C: spraying with clean water; T1: spraying with the inducer prepared according to the method marked T1 in Table 1; N1: spraying with the inducer prepared according to the method marked N1 in Table 1; N2: spraying with the inducer prepared according to the method marked N2 in Table 1. Before spraying, 1 day after spraying, 2 days after spraying, 3 days after spraying, 5 days after spraying, and 7 days after spraying represent the growth status before spraying the corresponding inducer, 1 day after spraying the inducer, 2 days after spraying the inducer, 3 days after spraying the inducer, 5 days after spraying the inducer, and 7 days after spraying the inducer, respectively. Under normal culture conditions, there were no significant differences in wheat growth phenotypes among the treatment groups in the short term, indicating that under non-stress conditions, each treatment had no significant effect on wheat growth. (Reference...) Figure 2In the table, CC indicates initial water spraying followed by normal growth; CD indicates initial water spraying followed by drought stress treatment; T1D indicates initial spraying with the inducer marked T1 in Table 1, followed by drought stress treatment; N1D indicates initial spraying with the inducer marked N1 in Table 1, followed by drought stress treatment; and N2D indicates initial spraying with the inducer marked N2 in Table 1, followed by drought stress treatment. Under drought conditions, compared with CC, spraying with drought-resistant inducer T1 significantly improved the growth phenotype under drought stress, while N1 and N2 had no significant effect and resulted in more wilting.

[0050] Reference Figures 3 to 7 It records the changes in different parameters of the plants under different spraying treatments. Specifically, Figure 3-7 In the bar chart, different letters represent the significance of the differences, p<0.05. Figure 3-7 In the statistical analysis of the experimental data, ANOVA and Duncan's multiple range test were used for significance testing, with a significance level of α = 0.05. The letter labels at the top of each bar followed these rules: groups labeled with completely different letters, such as 'a' and 'b', showed significant differences at the 0.05 level; groups labeled with the same letter, such as both labeled 'a', showed no significant differences. In the corresponding multiple comparisons, to accurately represent the significant relationships between groups, a shared letter labeling method was used, such as 'ab', 'bc', etc. This indicates that groups containing one shared letter, such as 'ab' and 'bc', showed no significant difference; while groups without any shared letters, such as 'ab' and 'c', showed a significant difference.

[0051] Specifically, Figure 3 In the table, CC indicates spraying with water; T1 indicates spraying with the inducer marked T1 in Table 1; N1 indicates spraying with the inducer marked N1 in Table 1; and N2 indicates spraying with the inducer marked N2 in Table 1. The photosynthetic rate and stomatal conductance of each group were compared on days 1, 2, and 3 after spraying. On day 1 after spraying with agent T1, this agent reduced stomatal aperture, which subsequently recovered to a level similar to the control. (Reference) Figure 4The treatments are denoted as follows: CC indicates initial water spraying followed by normal growth; CD indicates initial water spraying followed by drought stress treatment; T1D indicates initial spraying with the inducer marked T1 in Table 1, followed by drought stress treatment; N1D indicates initial spraying with the inducer marked N1 in Table 1, followed by drought stress treatment; and N2D indicates initial spraying with the inducer marked N2 in Table 1, followed by drought stress treatment. Drought stress was applied for 1, 2, 3, 5, and 7 days, and the effects of each treatment on photosynthetic rate and stomatal conductance were compared. Under drought stress, the photosynthetic rate and stomatal conductance of the CD treatment decreased significantly, and continued to decrease with increasing drought stress intensity. The T1D treatment increased photosynthetic rate and stomatal conductance compared to the CD treatment. Both the N1D and N2D treatments improved photosynthetic rate and stomatal conductance in the early stages of drought stress, but the effects were not significant in the later stages of drought stress, i.e., on the 7th day of drought stress. This indicates that the N1D and N2D treatments can help wheat cope with the challenge of insufficient water in the early stages of drought, but under continuous drought conditions, their effects are insufficient to improve photosynthesis and stomatal conductance in the long term.

[0052] Reference Figure 5 The different names in the table represent: C: spraying with water; T1: spraying with the inducer marked T1 in Table 1; N1: spraying with the inducer marked N1 in Table 1; N2: spraying with the inducer marked N2 in Table 1. The malondialdehyde (MDA) content is indicated after 1, 2, and 3 days of spraying, respectively. Spraying with T1, N1, and N2 had no significant effect on the MDA content, indicating that the prepared reagents, especially the drought-resistant inducer, did not cause oxidative damage. (Reference) Figure 6The different names represent: CC: initial water spraying followed by normal growth; CD: initial water spraying followed by drought stress treatment; T1D: initial spraying with inducer marked T1 in Table 1, followed by drought stress treatment; N1D: initial spraying with inducer marked N1 in Table 1, followed by drought stress treatment; N2D: initial spraying with inducer marked N2 in Table 1, followed by drought stress treatment. The proline content of plants was compared after 1, 3, and 5 days of drought stress. On day 1 of drought stress, the malondialdehyde (MDA) content under all treatments was not significantly higher than that under the CC treatment. As the number of drought days increased, the MDA content in plants treated with the CD method gradually increased compared to the CC treatment. On days 3 and 5 of drought stress, the T1D and N1D treatments significantly reduced the MDA content compared to the CD treatment, but the MDA content in the N2D spraying treatment did not decrease significantly compared to the CD treatment. This shows that T1D treatment, N1D treatment, especially T1D treatment, can significantly reduce the level of membrane peroxidation in wheat under drought stress.

[0053] refer to Figure 7 The different names in the table represent: C: water spraying; T1: spraying with the inducer marked T1 in Table 1; N1: spraying with the inducer marked N1 in Table 1; N2: spraying with the inducer marked N2 in Table 1. CC: initial water spraying followed by normal growth; CD: initial water spraying followed by drought stress treatment; T1D: initial spraying with the inducer marked T1 in Table 1 followed by drought stress treatment; N1D: initial spraying with the inducer marked N1 in Table 1 followed by drought stress treatment; N2D: initial spraying with the inducer marked N2 in Table 1 followed by drought stress treatment. The proline content in the plants was compared 1 day, 2 days, and 3 days after spraying, and the proline content in the plants 1 day, 3 days, and 5 days after drought stress. On day 1 of the T1 treatment, this application significantly increased the proline content in leaves. The proline content in the N1 and N2 treatments was not significantly different from that in the C treatment. With increasing time after spraying, the proline content in the T1 treatment gradually decreased to a level similar to the control. However, the N2 treatment significantly reduced proline content on day 3. Under drought stress, proline, as an osmotic regulator, significantly increased. On day 1 of drought, the T1D and N1D treatments significantly increased leaf proline content compared to the CD treatment. Proline content was directly proportional to drought severity; on day 5 of drought, the proline content in the T1D and N1D treatments was higher than that in the CD treatment. However, the N2D treatment showed proline levels similar to those in the CD treatment under drought stress, and even lower than the drought control CD treatment on days 3 and 5 of drought stress.

[0054] The above results show that the T1 drought-inducing agent provided in this application significantly promoted the photosynthetic rate and stomatal conductance of wheat under drought stress. MDA, as a marker of lipid peroxidation, reflects oxidative damage in plants under stress. The results indicate that T1 treatment significantly reduced MDA content under drought stress, suggesting that the drought-inducing agent can mitigate oxidative damage in plants and reduce the damage of reactive oxygen species (ROS) to cell membranes. Proline, as an important osmotic regulator, accumulates significantly under drought stress, helping plants maintain intracellular water balance. The statistical results also show that treatment with the T1 drought-inducing agent significantly increased the proline content in wheat, indicating that spraying with this drought-inducing agent can improve the osmotic regulation capacity of wheat by promoting proline synthesis.

[0055] In summary, the drought-inducing agent constructed in this application, with potassium persulfate as the core component, can establish a synergistic system based on stress signal pre-activation. Using potassium persulfate as the core component, it induces reactive oxygen species (ROS) to generate simulated early drought stress signals, triggering the plant's antioxidant defense system and simultaneously inducing proline biosynthesis, activating osmotic regulation mechanisms. Based on this early warning signal, and through the synergy of other components, it further achieves:

[0056] The water-responsive synergistic system—silicon fertilizer reduces stomatal conductance by regulating stomata, while potassium dihydrogen phosphate provides… Optimize stomatal movement response; the two work together to improve the plant's adaptability to water stress.

[0057] Metabolic synergistic support system—urea serves as a nitrogen source to promote the synthesis of osmotic protective proteins, sucrose maintains cell osmotic potential and provides a carbon source for glycolysis, and potassium dihydrogen phosphate provides phosphate ( These three elements—ATP (adenosine triphosphate) energy metabolism, osmotic regulation, and energy supply—form a synergistic network.

[0058] This system achieves a synergistic enhancement of drought resistance from signal perception to physiological execution through multi-level linkage: "potassium persulfate signal warning → synergistic water response of silicon fertilizer / potassium dihydrogen phosphate → metabolic network support of urea / sucrose / potassium dihydrogen phosphate". The synergistic effect of the inducer in this invention is not a simple superposition of the functions of each component, but rather a series of positive feedback and cascade amplification mechanisms formed by the stress warning signal of the core component, potassium persulfate, progressively introducing other components. This prompts the plant to develop an active regulatory mechanism in response to drought stress, thereby reducing the negative impact of drought on plant yield.

[0059] The foregoing has shown and described the basic principles, main features, and advantages of this application. Those skilled in the art should understand that this application is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this application. Various changes and modifications can be made to this application without departing from the spirit and scope thereof, and all such changes and modifications fall within the scope of this application as claimed. The scope of protection of this application is defined by the appended claims and their equivalents.

Claims

1. A drought-inducing agent, characterized in that, Each liter of the drought-inducing agent comprises: 50 μmol of potassium persulfate, 5 g of sucrose, 5 g of potassium dihydrogen phosphate, 5 g of urea, 2 ml of water-soluble silicon fertilizer, and 3 ml of earthworm enzyme.

2. A method for using a drought-inducing agent, characterized in that, 3-7 days before the seedlings face drought stress, the drought-inducing agent described in claim 1 is sprayed onto the leaves of the plants; the spraying amount is such that each leaf of the plant forms droplets of water that flow down; direct sunlight on the seedlings is avoided during spraying; the plant is wheat.

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