An anti-wrinkle composition containing recombinant collagen, and a preparation method and use thereof

Abstract

The application discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application thereof, and belongs to the technical field of cosmetics and skin care products. The composition contains the following active ingredients in percentage by mass: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract. The three kinds of recombinant humanized collagens and the space-bred desert algae extract form a complex system, and when the total mass ratio is in a specific interval of 1.3:1-4:1, the three kinds of recombinant humanized collagens and the space-bred desert algae extract can synergistically improve the skin barrier function. Based on the synergistic mechanism of the complex system, the composition disclosed by the application can effectively promote the skin moisture retention and anti-wrinkle ability, repair the skin barrier, and is suitable for the development and application of skin care products for anti-wrinkle, skin tightening and skin aging delay.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics and skin care products, in particular to an anti-wrinkle composition containing recombinant collagen and a preparation method and application thereof. BACKGROUND

[0002] With the improvement of living standards and the enhancement of skin care awareness, anti-wrinkle, firming and anti-aging skin care products have become an important development direction of the cosmetic market. The formation of wrinkles is mainly related to the decrease of collagen in the dermis, the degradation of the structure, and the weakening of the function of the basement membrane. At the same time, the damage of skin barrier function and water loss will accelerate skin aging. Therefore, modern anti-wrinkle skin care products need to comprehensively improve the collagen production capacity, repair the skin barrier, and enhance the moisturizing ability to achieve a more comprehensive anti-aging effect.

[0003] Existing anti-wrinkle products mostly use retinol, peptides, hyaluronic acid, natural extracts and other ingredients to play a role by promoting collagen production or improving water content. However, these ingredients still have deficiencies in safety, permeability and efficacy. On the one hand, some ingredients may cause irritation or allergic reactions to the skin, especially for sensitive skin; on the other hand, due to molecular structure or physical and chemical properties, these ingredients often have difficulty in effectively penetrating into the dermis, thereby affecting the anti-wrinkle effect. In addition, single component has limited mechanism, which is difficult to meet the multiple needs of anti-wrinkle, firming, moisturizing and antioxidant.

[0004] With the development of bioengineering technology, recombinant humanized collagen has become a research hotspot in the field of anti-wrinkle skin care due to its high homology with human collagen structure and good biocompatibility. Recombinant humanized type I, III and XVII collagen can not only be effectively absorbed by the skin, but also can play the roles of supplementing collagen, reconstructing dermal reticular structure and repairing basement membrane, thereby significantly improving the structural stability and elasticity of the skin. However, relying only on recombinant collagen is still difficult to achieve multi-dimensional synergistic anti-aging effect.

[0005] In view of this, the present application is proposed. SUMMARY

[0006] The present application aims to provide an anti-wrinkle composition containing recombinant collagen and a preparation method and application thereof. The anti-wrinkle composition promotes collagen production, enhances the moisturizing ability of the skin, and repairs the skin barrier through the synergistic effect of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen and space-bred desert algae extract, thereby significantly improving the elasticity, firmness and smoothness of the skin, effectively resisting skin aging, and overcoming the balance problem between skin permeability, efficacy and safety of traditional anti-aging products, and fundamentally solving the problem of skin aging.

[0007] The present application is implemented as follows:

[0008] In a first aspect, the present application provides an anti-wrinkle composition containing recombinant collagen, comprising the following components in mass percentage: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract, wherein the recombinant humanized type I collagen, the recombinant humanized type III collagen, and the recombinant humanized type XVII collagen constitute recombinant humanized collagen, and the mass ratio of the recombinant humanized collagen to the space-bred desert algae extract is 1.3-4:1.

[0009] In a second aspect, the present application provides a preparation method of the anti-wrinkle composition containing recombinant collagen according to any one of the preceding embodiments, comprising the following steps:

[0010] S1, weighing each component according to the formula;

[0011] S2, dissolving the water phase components at 80-90 ℃, and stirring and homogenizing until no particles are present;

[0012] S3, after cooling to below 45 ℃, adding the remaining components, stirring and homogenizing to obtain the anti-wrinkle composition containing recombinant collagen.

[0013] In a third aspect, the present application provides use of the anti-wrinkle composition containing recombinant collagen according to any one of the preceding embodiments in the preparation of cosmetics.

[0014] The present application has the following beneficial effects:

[0015] The present application realizes multiple synergistic effects by the combination of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, promotes collagen production, enhances the moisturizing ability of the skin, and repairs the skin barrier, thereby significantly improving the elasticity, firmness, and smoothness of the skin. The product of the present application has the effects of skin barrier repair, anti-aging care, collagen protection, collagen production promotion, and water retention and moisturizing, and is suitable for the research and application of skin care products for anti-wrinkle, firming, and anti-aging. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be described clearly and completely below. If the specific conditions are not specified in the embodiments, the conventional conditions or the conditions recommended by the manufacturer are used. If the reagents or instruments used are not specified by the manufacturer, they are all conventional products that can be purchased on the market.

[0017] This invention provides an anti-wrinkle composition containing recombinant collagen, comprising the following components by weight percentage: 0.01%~20% recombinant humanized type I collagen, 0.01%~20% recombinant humanized type III collagen, 0.01%~10% recombinant humanized type XVII collagen, and 0.01%~20% space-bred desert algae extract. The recombinant humanized type I collagen, recombinant humanized type III collagen, and recombinant humanized type XVII collagen constitute recombinant humanized collagen, and the mass ratio of recombinant humanized collagen to space-bred desert algae extract is 1.3-4:1.

[0018] This invention utilizes the synergistic effect of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract to promote collagen production, enhance skin's moisturizing ability, and repair the skin barrier. This significantly improves skin elasticity, firmness, and smoothness, effectively combats skin aging, and overcomes the traditional anti-aging product's challenge of balancing transdermal absorption, efficacy, and safety, thus fundamentally solving the problem of skin aging.

[0019] Among them, the extract of space-bred desert algae, as a novel active ingredient, is rich in polysaccharides, peptides, and antioxidants. It possesses excellent moisturizing capabilities, significantly promotes the proliferation of skin fibroblasts, and improves skin firmness. Simultaneously, this extract also has soothing and antioxidant effects, mitigating damage to the skin from free radicals and external stimuli. Therefore, developing an anti-wrinkle composition that synergistically combines multiple recombinant collagen proteins with the space-bred desert algae extract offers enhanced safety, stronger penetration, and more comprehensive anti-aging efficacy, meeting the urgent needs of current technological development.

[0020] The extract of space-bred desert algae is CODE2.0 of the space-bred algae. Hassallia sp. CODE2.0 (also known as Hassallia The extract of sp. P06), the CODE2.0 of the algae. Hassallia The accession number for sp. CODE2.0 is CCTCC NO: M 20242054, the accession date is September 23, 2024, the depositary institution is the China Center for Type Culture Collection (CCTCC), and the deposit address is Wuhan University, Wuhan, China. The aforementioned *Hassaela* CODE2.0 ( Hassallia sp. CODE2.0) is a self-developed raw material of Danzi Group Co., Ltd.

[0021] Further, the following components are included in a mass percentage: 0.05%-20% of recombinant humanized type I collagen, 0.05%-20% of recombinant humanized type III collagen, 0.05%-10% of recombinant humanized type XVII collagen, and 0.05%-20% of space-bred desert algae extract.

[0022] Further, the following components are included in a mass percentage: 0.05%-20% of recombinant humanized type I collagen, 0.05%-20% of recombinant humanized type III collagen, 0.05%-10% of recombinant humanized type XVII collagen, and 0.05%-20% of space-bred desert algae extract.

[0023] The anti-wrinkle composition containing recombinant collagen provided by the application is prepared by compounding recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, and has the following effects:

[0024] (1) Synergy of structural support and elastic repair:

[0025] Recombinant humanized type I collagen: as the main structural protein of the dermis layer of the skin, it can directly supplement the lost collagen fibers of the skin, construct a "skeleton" support structure, and improve the skin relaxation and wrinkles (such as marionette lines and forehead lines) caused by collagen loss.

[0026] (2) Synergy of skin barrier repair and anti-aging:

[0027] Recombinant humanized type XVII collagen: a transmembrane collagen, mainly distributed in the junction of epidermis and dermis (basement membrane zone), can strengthen the intercellular connection, promote the renewal and repair of the epidermis, and reduce the water loss and external stimulus invasion caused by barrier damage.

[0028] (3) Synergy of promoting endogenous collagen production

[0029] Recombinant humanized collagen type I, III, XVII: As an "exogenous collagen template", it can be absorbed by the skin or combined with fibroblast surface receptors to activate collagen synthesis signaling pathways (such as TGF-β pathway), induce the skin to synthesize more endogenous collagen, and achieve the dual effect of "external supplementation and internal promotion". Space breeding desert algae extract: Its antioxidant ingredients can improve the inflammatory environment of the skin and create favorable conditions for collagen production. Synergistic effect: The combination of direct exogenous collagen supplementation and endogenous collagen induction generates a virtuous cycle of "supplementation-activation-regeneration", which fundamentally increases the collagen content of the skin and delays wrinkle deepening.

[0030] (4) Metabolic synergy of moisturizing and anti-wrinkle

[0031] Space breeding desert algae extract: In addition to polysaccharide moisturizing, its natural moisturizing factors (such as glycerol and amino acids) can regulate skin hydration, maintain the water content of the stratum corneum, and prevent fine lines (such as dry lines) caused by dry skin. Recombinant humanized collagen (type I / III): Collagen fibers themselves have hygroscopic properties and can bind with water molecules to form "hydrated gel", enhancing the skin's water retention capacity and maintaining the skin's moist state with desert algae extract, reducing the causes of wrinkles caused by dryness. Synergistic effect: Moisturizing and collagen structure support each other - adequate moisture can maintain the extensibility of collagen fibers, while the integrity of the collagen network can prevent rapid water loss, forming a metabolic closed loop of "moisturizing-anti-wrinkle".

[0032] In an optional embodiment, the anti-wrinkle composition containing recombinant collagen further comprises one or more than two combinations of emulsifiers, antioxidants, emollients, thickeners, humectants, chelating agents, skin conditioners, perfumes, preservatives, pH adjusters, and the balance of ingredients is water. In addition, other functional ingredients can also be included as needed.

[0033] In an optional embodiment, the content of emulsifiers is 0-20%, the content of antioxidants is 0-10%, the content of emollients is 0-50%, the content of thickeners is 0.01%-8%, the content of humectants is 0.01%-40%, the content of chelating agents is 0.01%-1%, the content of skin conditioners is 0-50%, the content of perfumes is 0-1%, the content of preservatives is 0.01-3%, and the content of pH adjusters is 0-3%, based on the total mass of the anti-wrinkle composition containing recombinant collagen being 100%.

[0034] More specifically, the content of the emulsifier is 0~10%, the content of the antioxidant is 0~5%, the content of the emollient is 0~20%, the content of the thickening agent is 0.01%~1%, the content of the humectant is 0.01%~20%, the content of the chelating agent is 0.01%~0.08%, the content of the skin conditioning agent is 0~20%, the content of the odorant is 0~0.2%, the content of the preservative is 0.01~2%, and the content of the pH adjuster is 0~2%, based on the total mass of the anti-wrinkle composition containing the recombinant collagen being 100%.

[0035] Further, the emulsifier includes, but is not limited to, one or more of PEG-100 stearate, polyglyceryl-3 methylglucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivate, sodium methyl stearoyl taurate, sodium methyl stearoyl taurate, hydrogenated lecithin, sorbitan olivate, and polyglyceryl-3 distearate; it should be noted that, as the emulsifier, any emulsifier that can form a water-in-oil (W / O) type, oil-in-water (O / W) type emulsified anti-wrinkle cosmetic composition can be used without particular limitation.

[0036] In some embodiments, the antioxidant includes, but is not limited to, one or more of vitamin E and derivatives thereof, vitamin C and derivatives thereof, ubiquinone, and fullerene; preferably, the antioxidant is selected from vitamin E and derivatives thereof.

[0037] In some embodiments, the emollient includes, but is not limited to, one or more of glyceryl tri(ethylhexanoate), caprylic / capric triglyceride, pentaerythrityl tetraethylhexanoate, butyl octylsalicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, cetearyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, dimethicone, cetearyl alcohol, dibutyl adipate, C12~15 alcohol benzoate, dicaprylyl carbonate, coco-caprylate / caprate, dimethicone, octyl dimethicone, cyclopentasiloxane, and phenyl trimethicone; it should be noted that, as the emollient, other emollients commonly used in the cosmetic field can be used without particular limitation. Preferably, the emollient is selected from one or more of C12~15 alcohol benzoate, dimethicone, and rice germ oil.

[0038] In some embodiments, as the water phase ingredient, the thickening agent includes, but is not limited to, one or more of xanthan gum, acrylates / C10-30 alkyl acrylate crosspolymer, carbomer, ammonium acryloyldimethyltaurate / VP copolymer, sodium acrylatesodium acryloyldimethyl taurate copolymer; preferably, the thickening agent is selected from one or more of acrylates / C10-30 alkyl acrylate crosspolymer, ammonium acryloyldimethyltaurate / VP copolymer, sodium acrylatesodium acryloyldimethyl taurate copolymer.

[0039] In some embodiments, the humectant includes, but is not limited to, one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe extract, jojoba oil, polyethylene glycol / polypropylene glycol-14 / 7 dimethyl ether, xylitol, sorbitol; as the water phase ingredient, any humectant commonly used in the cosmetic field can be used without particular limitation. Preferably, the humectant is selected from glycerin.

[0040] In some embodiments, the chelating agent includes, but is not limited to, one or more of disodium EDTA, tetrasodium EDTA, sodium phytate.

[0041] In some embodiments, the skin conditioning agent includes, but is not limited to, one or more of vitamin E having a damaged skin repair effect, radix scutellariae extract, bisabalol, radix scutellariae extract, Prunus armeniaca extract, dextran, vitamin B5.

[0042] In some embodiments, the fragrance includes, but is not limited to, one or more of perfume, essential oil.

[0043] In some embodiments, as the water phase ingredient, the preservative includes, but is not limited to, one or more of phenoxyethanol, ethylhexylglycerin, hexylene glycol, pentylene glycol.

[0044] In some embodiments, as the water phase ingredient, the pH adjuster includes, but is not limited to, one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethyl propanol.

[0045] Further, the present application provides a preparation method of the above anti-wrinkle composition containing recombinant collagen, comprising the following steps:

[0046] S1, weighing each component according to the formula;

[0047] S2, dissolving the water phase component at 80-90 ℃, stirring and homogenizing until no particles are present;

[0048] S3, after cooling to below 45 ℃, adding the remaining components, stirring and homogenizing to prepare the anti-wrinkle composition containing recombinant collagen.

[0049] The preparation method is simple, easy to operate, and suitable for mass production.

[0050] In addition, the application further provides application of the anti-wrinkle composition containing the recombinant collagen in preparation of cosmetics.

[0051] In an optional embodiment, the cosmetic is any one of skin care water, essence, emulsion, cream, mask, sunscreen milk, BB cream and sunscreen cream.

[0052] The features and performances of the application are further described in detail below in combination with examples.

[0053] I. Application Examples

[0054] Example 1

[0055] The embodiment provides an anti-wrinkle composition containing recombinant collagen, which comprises the following components in percentage by mass:

[0056] B phase: 0.05% of recombinant humanized type I collagen, 0.05% of recombinant humanized type III collagen, 0.05% of recombinant humanized type XVII collagen, 0.05% of space-bred desert algae extract, and 0.5% of preservative;

[0057] A phase: 0.05% of chelating agent, 0.5% of thickening agent, 5% of humectant, and the rest of deionized water.

[0058] The chelating agent is EDTA-2Na, the thickening agent is sodium acrylate / sodium acryloyldimethyl taurate copolymer, the humectant is glycerol, and the preservative is phenoxyethanol.

[0059] A specific preparation method is as follows:

[0060] S1, ingredients are weighed according to the formula;

[0061] S2, A phase components are dissolved at 85 DEG C, and stirred and homogenized until no particles are present;

[0062] S3, after cooling to below 45 DEG C, B phase components are added and stirred uniformly to prepare the anti-wrinkle composition.

[0063] Examples 2-6

[0064] Examples 2-6 and Comparative Examples 1-2 provide an anti-wrinkle composition containing recombinant collagen, which is different from Example 1 in that the specific component usage is different, and the others remain unchanged, and the specific usage of each component is shown in the following Table 1.

[0065] Table 1 Formulation of Examples 1-6 and Comparative Examples 1-2

[0066]

[0067] II. Application Comparative Examples

[0068] Based on Example 3, a plurality of comparative examples are set up by canceling one or more components or replacing one component, around the four important components of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract, namely Comparative Example 3 to Comparative Example 16.

[0069] Among them, Comparative Example 3 to Comparative Example 6 omit any one of the four components of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract.

[0070] Comparative Example 7 to Comparative Example 10 omit any two of the four components of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract.

[0071] Comparative Example 11 does not add any of the four components of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract.

[0072] Comparative Example 12 to Comparative Example 15 respectively replace the four components of recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen, and space-bred desert algae extract with conventional raw materials, that is, Comparative Example 12 uses conventional animal-derived type I collagen to replace recombinant humanized type I collagen, Comparative Example 13 uses conventional animal-derived type III collagen to replace recombinant humanized type III collagen, Comparative Example 14 uses conventional animal-derived type XVII collagen to replace recombinant humanized type XVII collagen, and Comparative Example 15 uses conventional trehalose to replace space-bred desert algae extract.

[0073] In Comparative Example 16, the amount of key ingredients exceeds the limited range of the present application.

[0074] III. Experimental Test

[0075] Experiment 1: Elastase activity inhibition test

[0076] Elastase activity inhibition test is of great significance in anti-aging research, as it directly evaluates the inhibitory ability of skin care products on elastin degradation. Elastin is a key component in maintaining skin elasticity and firmness, although it accounts for a small proportion in the dermis, it plays an important role in maintaining skin elasticity, preventing wrinkles and sagging. However, with age, elastin is gradually broken down by elastase, leading to loss of skin elasticity, wrinkles, sagging and other signs of aging. Through elastase activity inhibition test, researchers can evaluate the inhibitory effect of active ingredients in skin care products on elastase activity. This test can reveal whether the skin care composition is effective in preventing or slowing down the degradation of elastin, thus reflecting the potential of the product in maintaining skin elasticity and delaying aging. Inhibition of elastase activity is an important mechanism to delay wrinkle formation and maintain skin firmness, therefore it has key evaluation value in anti-aging research.

[0077] Therefore, elastase activity inhibition test is adopted, and the specific test process is as follows:

[0078] Take the compositions prepared in Examples 1-6 and Comparative Examples 1-16, respectively, dilute with deionized water to a concentration of 5% sample solution, and backup for use. Add 1 mL of sample solution and 1 mL of elastase solution to a 5 mL centrifuge tube, mix thoroughly, then add 1 mL of substrate solution, continue to react at 37°C for 30 min; after incubation, centrifuge at 1000 rpm for 5 min, take 200 uL of supernatant to a 96-well plate, detect the absorbance at 405 nm wavelength with a microplate reader. At the same time, set up blank substrate group (replace substrate with equal volume of 10% DMSO), 100% enzyme activity control group and blank control group. Each group is tested in triplicate, with seviteron sodium as the positive control. The calculation formula of elastase inhibition rate is as follows:

[0079] Elastase inhibition rate = [1- (Y1-Y2) / (Y3-Y4)] x 100%

[0080] In the formula: Y1 is the absorbance value of test sample + elastase solution + substrate solution, Y2 is the absorbance value of test sample + elastase solution + 10% DMSO, Y3 is the absorbance value of PBS buffer solution + elastase solution + substrate solution, Y4 is the absorbance value of PBS buffer solution + elastase solution + 10% DMSO. The results of elastase activity inhibition test are shown in Table 2 below.

[0081] Table 2 Results of elastase activity inhibition test

[0082]

[0083] The results of Table 2 show that the anti-wrinkle compositions of Examples 1-6 all exhibit excellent elastase inhibitory activity, with the highest clearance rate reaching 76.32%.

[0084] As can be seen from the data of Comparative Examples 1-6 and Comparative Examples 1-16:

[0085] The comprehensive performance of the example group is significantly better than that of the comparative example group, and the core indicators are as follows: the elastase inhibition rate of Examples 1-6 is significantly higher than that of all comparative examples. Among them, Comparative Examples 12-15 replace a single component in the composition of the application with a functionally similar component, resulting in a significant decrease in the inhibition rate compared to the example group, directly confirming the irreplaceability of the synergistic effect of the four components of the application; Comparative Example 16 also shows a significant decrease in the inhibition rate compared to the example group due to the use of key components beyond the limited range of the application.

[0086] Regarding the relationship between concentration and effect, research shows that in a system where the four components coexist, the elastase inhibition rate and anti-wrinkle effect show a dose-dependent characteristic - within the concentration range defined in the application, the higher the concentration, the higher the elastase inhibition rate, and the anti-wrinkle effect is simultaneously enhanced; but when the concentration exceeds this range (for example, Comparative Example 16), as the concentration further increases, the elastase inhibition rate decreases, and the anti-wrinkle effect decreases.

[0087] As can be seen from Comparative Examples 3-15, the absence or replacement of a single component leads to a decrease in effect: if any component is missing, the inhibition rate decreases by more than 50% compared to the complete formula, and the anti-wrinkle effect is significantly weakened; among them, the space-bred desert algae extract is an important support for improving the effect, and the inhibition rate is significantly reduced when it is missing or replaced by ordinary algae extract; the "space-bred" process is the key to its high activity. After replacing the recombinant humanized collagen with ordinary collagen and the space-bred algae extract with algae extract, the inhibition rate decreased by 42.7% and 38.5%, respectively, confirming that the recombinant humanized structure and the unique active ingredients of space-bred algae are the core of the effect; when a single component is used alone, the elastase inhibition rate is less than 20%, and the anti-wrinkle effect is weak. At the same time, the recombinant humanized collagen and the space-bred desert algae extract have a synergistic effect, and only when the ratio of the two is 1.3-4:1, the inhibition rate remains at a high level of 35.24%-76.32%; if the ratio exceeds this range (for example, Comparative Example 1 and Comparative Example 2), the inhibition effect will be significantly worse.

[0088] Conclusion: The recombinant humanized type I, type III, and type XVII collagen proteins and the space-bred desert algae extract form a complete anti-wrinkle system through component complementation and functional synergy, and the absence or replacement of any component will disrupt the synergistic effect, and the two components must be within the ratio range of 1.3-4:1 to exert the optimal synergistic effect, verifying that the coexistence of the four components and the specific ratio of the components are the key mechanisms for achieving high-efficiency anti-wrinkle.

[0089] Experiment 2: Trans-epidermal water loss rate analysis of skin

[0090] Trans-epidermal water loss rate (TEWL) of skin is measured by Tewameter, TM300, which is an important parameter reflecting the water loss and barrier of skin. The lower the measured value, the better the barrier function of the skin. The detection method of trans-epidermal water loss rate analysis of skin is as follows:

[0091] Subject selection criteria: healthy female subjects aged 20 to 50 years old; and the subjects are positive after 10% lactic acid sting test (judged as sensitive skin); with slight redness on the face; 110 people, the compositions obtained in Examples 1-6 and Comparative Examples 1-16 are used as samples for use evaluation, 5 people are randomly selected for each sample.

[0092] Use site: face

[0093] Use frequency: use 1 time each morning and evening, continuously use for 28 days, 1.2 g each time.

[0094] Test environment conditions: temperature 21±1℃, relative humidity 50±5%.

[0095] Test design: single blind, random, self before and after control, face (according to the random table, the side opposite to the lactic acid solution application area is selected).

[0096] Evaluation method details: trans-epidermal water loss rate analysis of skin, the results are shown in Table 3 below.

[0097] Table 3: Change rate data of trans-epidermal water loss of facial skin

[0098]

[0099] From the results in Table 3, the experimental results show that there is a strict synergistic ratio range between the total amount of recombinant humanized type I, type III and type XVII collagen and the amount of space-bred desert algae extract. When the two are compounded at a mass ratio of 1.3:1 to 4:1, the composition shows a significant effect on reducing trans-epidermal water loss (TEWL) compared to other ratios (TEWL reduction is greater than 20%). This ratio is the critical range that triggers the synergistic effect. Beyond this range, the efficacy of the composition is significantly reduced, and even may have a reverse effect (as shown in Comparative Examples 1 and 2). This proves that the ratio relationship is an indispensable technical feature of the technical solution of the present application.

[0100] Necessity of the combination of active ingredients: By setting up the comparative examples of canceling single or multiple components (comparative examples 3-11), it is proved that the recombinant humanized type I collagen, recombinant humanized type III collagen, recombinant humanized type XVII collagen and space-bred desert algae extract together constitute the necessary technical features to achieve the excellent effect. The lack of any one or several components, or in any two-component combination, cannot achieve the TEWL reduction effect achieved by the technical scheme of the present application. This shows that the four active ingredients constitute a functionally interdependent and indivisible synergistic system, and the technical effect is not a simple addition of the functions of each component.

[0101] Non-substitutability of specific raw material sources: By setting up comparative examples of replacing components (comparative examples 12-15), it is proved that the specific sources of the active ingredients are the key to achieving the technical effect. After using conventional animal-derived collagen instead of recombinant humanized collagen, or using ordinary algae extract instead of space-bred desert algae extract, the efficacy of the composition returns to the ordinary level, which is significantly different from the technical scheme of the present application. The results prove that the unique properties of the components endowed by the specific sources of "recombinant humanized" and "space-bred" are the basis for the realization of the synergistic effect, and this technical effect cannot be achieved by conventional raw materials. The concentration of the components in comparative example 16 is beyond the scope of the present application, and as the concentration further increases, the effect of reducing transepidermal water loss decreases.

[0102] In summary, the core of the technical scheme of the present application is to find and verify a complex system composed of recombinant humanized type I, III and XVII collagen and space-bred desert algae extract of specific sources, and this system can only produce unexpected synergistic effect in improving skin barrier function when the total mass ratio of the active ingredients is within the specific range of 1.3:1 to 4:1. The combination of active ingredients, specific ratio range and raw material sources in the technical scheme are interrelated and work together to form a complete, non-obvious and significantly effective technical whole, which has outstanding substantial features and significant progress compared with the prior art.

[0103] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. An anti-wrinkle composition containing a recombinant collagen, characterized in that, The composition comprises the following components in percentage by mass: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract, the recombinant humanized type I collagen, the recombinant humanized type III collagen, and the recombinant humanized type XVII collagen constitute a recombinant humanized collagen, the mass ratio of the recombinant humanized collagen to the space-bred desert algae extract is 1.3-4:1, the space-bred desert algae extract is an extract of space-bred Haematococcus CODE2.0, and the accession number of the Haematococcus CODE2.0 (Haematococcus sp. CODE2.0) is CCTCC NO: M 20242054. Hassallia ​ 2. The anti-wrinkle composition containing recombinant collagen according to claim 1, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The composition comprises the following components in mass percentage: 0.05%-20% of recombinant humanized type I collagen, 0.05%-20% of recombinant humanized type III collagen, 0.05%-10% of recombinant humanized type XVII collagen, and 0.05%-20% of space-bred desert algae extract.

3. The anti-wrinkle composition containing recombinant collagen according to claim 1, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The composition comprises the following components in mass percentage: 0.2%-20% of recombinant humanized type I collagen, 0.5%-20% of recombinant humanized type III collagen, 0.1%-10% of recombinant humanized type XVII collagen, and 0.2%-20% of space-bred desert algae extract.

4. The anti-wrinkle composition containing recombinant collagen according to claim 1, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The anti-wrinkle composition containing recombinant collagen further comprises one or more than two combinations of emulsifier, antioxidant, emollient, thickening agent, humectant, chelating agent, skin conditioning agent, odorant, preservative, pH regulator, and a residual ingredient of water.

5. The anti-wrinkle composition containing recombinant collagen according to claim 4, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The anti-wrinkle composition containing recombinant collagen further comprises one or more than two combinations of emulsifier, antioxidant, emollient, thickening agent, humectant, chelating agent, skin conditioning agent, odorant, preservative, pH regulator, and a residual ingredient of water.

6. The anti-wrinkle composition containing recombinant collagen according to claim 4, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The anti-wrinkle composition containing recombinant collagen further comprises one or more than two combinations of emulsifier, antioxidant, emollient, thickening agent, humectant, chelating agent, skin conditioning agent, odorant, preservative, pH regulator, and a residual ingredient of water.

7. The anti-wrinkle composition containing recombinant collagen according to any one of claims 4 to 6, wherein the collagen is a collagen of which the amino acid sequence is represented by SEQ ID NO:

1. The emulsifier is selected from one or more of PEG-100 stearate, polyglyceryl-3 methylglucose distearate, potassium cetyl phosphate, cetearyl glucoside, cetearyl olivate, sodium methylisostearoyl taurate, sodium methylisostearoyl taurate, hydrogenated lecithin, sorbitan olivate, and polyglyceryl-3 distearate; and / or, the antioxidant is selected from one or more of vitamin E and derivatives thereof, vitamin C and derivatives thereof, ubiquinone, and fullerene; and / or, the emollient is selected from one or more of glyceryl tri (ethylhexanoate), caprylic / capric triglyceride, pentaerythrityl tetra (ethylhexanoate), butyloctyl salicylate, diethoxyethyl succinate, isododecane, rice germ oil, hydrogenated palm kernel oil, coca seed cetyl ethylhexanoate, isopropyl myristate, isopropyl palmitate, diisopropyl sebacate, dimethicone, cetyl stearyl alcohol, dibutyl adipate, C12-15 alcohol benzoate, dicaprylyl carbonate, coco-caprylate / caprate, dimethicone, octyl dimethicone, cyclotetrasiloxane, and phenyl trimethicone; and / or, the thickening agent is selected from one or more of xanthan gum, acrylates / C10-30 alkyl acrylate crosspolymer, carbomer, acryloyldimethyltaurine ammonium / VP copolymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer; and / or, the humectant is selected from one or more of glycerin, propylene glycol, seaweed extract, sodium hyaluronate, aloe extract, jojoba oil, polyethylene / polypropylene glycol-14 / 7 dimethyl ether, xylitol, sorbitol; and / or, the chelating agent is one or a combination of two or more of disodium EDTA, tetrasodium EDTA, sodium phytate; and / or, the skin conditioning agent is one or more of vitamin E, scute extract, bisabalol, scute root extract, Prunus armeniaca (apricot) kernel extract, dextran, vitamin B5; and / or, the odorant includes one or both of fragrance and essential oil; and / or, the preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, hexylene glycol, pentylene glycol; and / or, the pH adjuster is selected from one or more of arginine, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethyl propanol.

8. The method of claim 1 to 7, wherein the anti-wrinkle composition comprising the recombinant collagen is prepared by the steps of: (a) mixing the recombinant collagen with a pharmaceutically acceptable carrier; and (b) sterilizing the mixture of step (a). The method comprises the following steps: S1. Weighing each component according to the formula; S2. Dissolving the water phase components at 80-90 ℃, and stirring and homogenizing until there are no particles; S3. After cooling to below 45 ℃, adding the remaining components, stirring and homogenizing to obtain the anti-wrinkle composition containing recombinant collagen.

9. Use of the anti-wrinkle composition containing recombinant collagen according to any one of claims 1-7 in the preparation of a cosmetic product.

10. Use according to claim 9, characterized in that, The cosmetic product is any one of skin care water, essence, emulsion, cream, mask, sunscreen, BB cream, and sunscreen. The cosmetic product is any one of skin care water, essence, emulsion, cream, mask, sunscreen, BB cream, and sunscreen.

Images