Antibacterial agent

Abstract

The invention provides a new application of the chlorine hydrozincite. The antibacterial agent according to the present invention is an antibacterial agent for at least one type of skin resident bacteria selected from the group consisting of Corynebacterium genus, Moraxella genus, and Malassezia genus, said antibacterial agent containing chlorine hydrozincite.

Description

Technical Field

[0001] This invention relates to antibacterial agents, and more particularly to antibacterial agents used in combination with deodorant cosmetics or scalp care cosmetics (to prevent dandruff, itching, and scalp odor). Background Technology

[0002] The skin is home to a variety of microorganisms known as resident flora, forming a microbiome. Within this microbiome, there are microorganisms that maintain skin health, as well as those that cause adverse effects such as rough skin or body odor. The proportions of these microorganisms vary depending on skin condition and overall health. Among these adverse resident flora, Corynebacterium, Moraxella, and Malassezia are well-known for causing body odor or scalp odor by breaking down components in sweat or sebum or by synthesizing odor compounds during proliferation. Corynebacterium is famous for causing underarm odor and is also a cause of senile odor, scalp odor, and foot odor. Moraxella is responsible for the musty odor of laundry detergent (dried odor). Furthermore, Malassezia is known to cause seborrheic alopecia when it proliferates excessively on the scalp, contributing to scalp odor, inflammation, dandruff, and itching. Therefore, technologies are being developed to suppress unpleasant odors such as body odor, scalp odor, and musty odor by inhibiting the proliferation and activity of these microorganisms. For example, Patent Document 1 proposes a scheme for an anti-axillary odor agent and an anti-Malassezia agent using a fat-soluble extract of Buttercup spp. as the active ingredient, while Patent Document 2 proposes a scheme for an inhibitor of musty odor generated by Morassezia bacteria using a straight-chain aliphatic aldehyde with a specific number of carbon atoms as the active ingredient.

[0003] On the other hand, Simonkolleite is a zinc hydroxide hydrate composed of zinc, hydroxyl groups, and chlorine, with the chemical formula Zn5(OH)8Cl2·(H2O). n This compound is indicated by [reference needed]. It is a white crystalline powder insoluble in water and organic solvents, also known as basic zinc chloride or zinc hydroxide chloride. The natural mineral zinc chloride ore was discovered as a new mineral in 1985 and named after Werner Simon and Kurt Kolle, who collected mineral samples of it. To date, zinc chloride ore has been primarily utilized as a major component of tetrabasic zinc chloride (TBZC), a feed additive for animals, and is considered an ideal nutritional supplement for animals.

[0004] In recent years, research and development have been conducted on new uses and industrial manufacturing methods for this zinc chloride ore. For example, Patent Document 3 reports a technique for using zinc chloride ore as a skin wound treatment agent, and Patent Document 4 reports a method for manufacturing zinc chloride ore that can be used as a pharmaceutical raw material.

[0005] Existing technical documents Patent documents Patent Document 1: Japanese Patent Application Publication No. 2021-161043 Patent Document 2: Patent No. 5852313 Patent Document 3: Patent No. 6185215 Patent Document 4: International Publication No. 2018 / 105738 Summary of the Invention

[0006] The problem that the invention aims to solve In the aforementioned Patent Document 1, it was reported that the fat-soluble extract of Buttercup salsa has antibacterial effects on microorganisms that cause body odor or scalp odor. In Patent Document 2, it was reported that linear aliphatic aldehydes have the ability to inhibit the production of 4-methyl-3-hexenoic acid, a substance that causes musty odor, by microorganisms. However, the effect of zinc chloride ore on this type of microorganism has not been studied to date, and its effectiveness is unknown.

[0007] In addition, patent document 3 reports the use of zinc chloride mineral as a skin wound treatment agent, but so far there has been no research on the use of zinc chloride mineral for suppressing body odor and scalp odor, preventing dandruff and itching of the scalp, and its effectiveness is unknown.

[0008] Therefore, the present invention was made in view of the above-mentioned problems, and its object is to provide new uses for chlorinated zinc ore.

[0009] Problem-solving methods The inventors of this application conducted in-depth research on the novel functions of zinc chloride ore, and discovered that zinc chloride ore has antibacterial effects against microorganisms that cause body odor, scalp odor, and musty odor. Based on this discovery, this invention was completed. To solve the above-mentioned problems, the antibacterial agent of this invention is an antibacterial agent targeting at least one resident skin bacterium selected from the group consisting of Corynebacterium, Moraxella, and Malassezia, and contains zinc chloride ore as an active ingredient. Zinc chloride ore has an antibacterial effect that inhibits the proliferation of resident skin bacteriums of Corynebacterium, Moraxella, and Malassezia. Therefore, it can inhibit the activity and proliferation of microorganisms that cause such body odor, scalp odor, and musty odor.

[0010] Furthermore, the antibacterial agent of the present invention preferably uses zinc chloride mineral as nanoparticles. Therefore, when used in cosmetics, the roughness caused by the insoluble zinc chloride mineral as an antibacterial ingredient is reduced, and it is less likely to leave a white residue on the skin after application, resulting in a smooth feel and an ideal appearance after use. In this specification, nanoparticles refer to the 50% cumulative particle size (D5) on the small particle side of the particle size distribution based on volumetric laser diffraction scattering. 50 () refers to particulate matter with a wavelength greater than 1 nm and less than 800 nm.

[0011] Furthermore, in the antibacterial agent of the present invention, zinc chloride ore is preferably the 50% cumulative particle size (D) in the particle size distribution based on the volume reference of laser diffraction scattering method. 50 These are nanoparticles smaller than 250 nm. They are produced by processing zinc chloride ore into D... 50 With nanoparticles smaller than 250nm, when this antibacterial agent is used in cosmetics, the roughness caused by zinc chloride minerals is eliminated, and no white residue is left on the skin even after application, thus presenting a smooth feel and an ideal appearance after use.

[0012] Furthermore, the deodorant cosmetic of the present invention contains the aforementioned antibacterial agent. Since the aforementioned antibacterial agent inhibits the activity and proliferation of Corynebacterium, Moraxella, and Malassezia microorganisms that cause body odor, scalp odor, and stuffy odor, a deodorant cosmetic capable of inhibiting the generation of unpleasant odors such as body odor, scalp odor, and stuffy odor can be obtained, and reducing these unpleasant odors.

[0013] Furthermore, the scalp care cosmetic of the present invention contains the aforementioned antibacterial agent. Since the aforementioned antibacterial agent has antibacterial activity against Malassezia fungi, a scalp care cosmetic can be obtained that can suppress scalp odor, inflammation, dandruff, and itching, and maintain scalp health.

[0014] The antibacterial agent of the present invention is an antibacterial agent targeting at least one microorganism selected from the group consisting of *Corynebacterium*, *Moraxella*, *Malassezia*, *Staphylococcus*, *Pseudomonas*, *Candida*, and *Aspergillus*, and contains zinc chloride ore as an active ingredient. Zinc chloride ore has antibacterial activity that inhibits the proliferation of *Corynebacterium*, *Moraxella*, *Malassezia*, *Staphylococcus*, *Pseudomonas*, *Candida*, and *Aspergillus* microorganisms. Therefore, it can be used as an antibacterial agent to inhibit the activity and proliferation of these microorganisms.

[0015] Furthermore, the antibacterial product of the present invention contains the aforementioned antibacterial agent. By including this antibacterial agent in various products, antibacterial products with antibacterial activity against the aforementioned microorganisms can be easily obtained.

[0016] Invention Effects According to the present invention, it is possible to provide antibacterial agents, deodorant cosmetics, scalp care cosmetics, and antibacterial products with the following excellent effects.

[0017] (1) Since zinc chloride mineral has antibacterial properties that inhibit the proliferation of resident skin bacteria such as Corynebacterium, Moraxella and Malassezia, it can inhibit the activity and proliferation of microorganisms that cause body odor, scalp odor and stuffy odor, as well as microorganisms that cause scalp odor, scalp inflammation, dandruff and itching.

[0018] (2) Since zinc chloride ore inhibits the activity and proliferation of microorganisms that cause body odor, scalp odor and stuffy odor, as well as microorganisms that cause scalp odor, scalp inflammation, dandruff and itching, it is suitable as a functional ingredient in deodorant cosmetics or scalp care cosmetics.

[0019] (3) Since zinc chloride ore has antibacterial properties that inhibit the proliferation of microorganisms such as Corynebacterium, Moraxella, Malassezia, Staphylococcus, Pseudomonas, Candida and Aspergillus, antibacterial products can be easily obtained by including zinc chloride ore in materials or articles that are to be endowed with antibacterial properties. Attached Figure Description

[0020] Figure 1(a) is a graph showing the antibacterial effect of the paper product treated with zinc chloride ore in Example 4, and Figure 1(b) is a graph showing the antibacterial effect of the fabric product treated with zinc chloride ore in Example 4. Detailed Implementation

[0021] The following describes the antibacterial agents, deodorant cosmetics, scalp care cosmetics, and antibacterial products of the present invention.

[0022] The antibacterial component in the antibacterial agents, deodorants, cosmetics for scalp care, or antibacterial products of the present invention, zinc chloride ore, refers to Zn5(OH)8Cl2·(H2O). n This refers to a white crystalline powder of zinc hydroxide hydrate. Zinc hydroxide hydrate is typically circulated in industrially manufactured forms, such as those described in Patent Document 4 above. For example, zinc hydroxide hydrate from JFE Minerals Co., Ltd. is preferred, but zinc hydroxide hydrate obtained by other manufacturing methods or from natural mineral sources may also be used. Furthermore, the zinc hydroxide hydrate used may be a substance with the same grain phase, a different substance (heterogeneous phase), or a mixture thereof.

[0023] In this invention, "antimicrobial" effect refers to the ability to inhibit the proliferation of microorganisms targeted for antimicrobial activity, compared to a control group without the antimicrobial agent of this invention. The antimicrobial effect can be measured using known antimicrobial testing methods as shown in the examples described later.

[0024] The antibacterial target of the antibacterial agent involved in this invention is at least a microorganism that causes body odor, scalp odor, stuffy odor, etc., and is a member of the genus Corynebacterium ( ). Corynebacterium Bacteria, Moraxella genus ( Moraxella ) bacteria or Malassezia ( Malassezia Fungi. The antibacterial agent involved in this invention has excellent antibacterial activity against resident skin bacteria that cause body odor, etc. Therefore, it can be used in cosmetics for suppressing body odor (i.e., deodorant) or cosmetics for suppressing scalp odor and preventing dandruff and itching. The bacteria are not particularly limited to the genus *Corynebacterium*, for example, *Corynebacterium zoster* (…). Corynebacterium striatum Corynebacterium jejuni ( Corynebacterium jeikeium ), Corynebacterium reproductiveum ( Corynebacterium genitalium ), etc. Additionally, the genus *Moraxella* is not specifically defined, but includes *Moraxella osloensis* (…). Moraxella osloensis Additionally, as a fungus of the genus Malassezia, there are no specific limitations; for example, Malassezia furfur (Malassezia spp.) Malassezia furfur ), Spherical Malassezia ( Malassezia globosa ), limiting Malassezia ( Malassezia restricta )wait.

[0025] Furthermore, in addition to the aforementioned Corynebacterium, Moraxella, or Malassezia bacteria, Staphylococcus also targets the antimicrobial agents involved in this invention. Staphylococcus Bacteria, Pseudomonas spp. Pseudomonas Bacteria, Candida spp. Candida ) fungi or Aspergillus genus ( Aspergillus Fungi. The antimicrobial agent involved in this invention has antimicrobial activity against these microorganisms, and therefore can be used in antimicrobial agents used to inhibit the proliferation of these microorganisms. By including this antimicrobial agent in various products, antimicrobial products with antimicrobial activity against the aforementioned microorganisms can be easily obtained. As for Staphylococcus bacteria, there is no particular limitation; for example, Staphylococcus aureus (…) Staphylococcus aureus ), etc. Additionally, there is no specific limitation regarding Pseudomonas bacteria; it includes *Pseudomonas aeruginosa* (…). Pseudomonas aeruginosa Additionally, as a fungus of the genus Candida, Candida albicans can be cited as an example. Candida albicans Aspergillus fungi, such as Aspergillus brasiliensis ( ), can be cited as examples. Aspergillus brasiliensis )wait.

[0026] The zinc chloride mineral contained in the antibacterial agent of the present invention, especially when used as an antibacterial agent in deodorant cosmetics or scalp care cosmetics, is preferably in nanoparticle form to reduce the roughness caused by zinc chloride mineral when applied to the skin or scalp and to reduce the white residue left on the skin after application. In this specification, nanoparticles refer to the 50% cumulative particle size (D5) on the small particle side of the particle size distribution based on the volume scattering method of laser diffraction. 50 The material is a particulate substance with a particle size of 1 nm or larger and less than 800 nm. In this invention, from the viewpoint of further eliminating the roughness and white residue on the skin caused by zinc chloride ore, it is preferable to prepare zinc chloride ore with a cumulative particle size (D) of 50%. 50 The particles are nanoparticles smaller than 500 nm, and more preferably, zinc chloride mineral is further processed to have a cumulative particle size (D) of 90%. 90 ) refers to nanoparticles below 800nm.

[0027] The amount of zinc chloride ore contained in, added to, combined with, or administered to the antibacterial target in the antibacterial agent of the present invention can be appropriately set according to the target antibacterial effect, the target of addition / administration, and the method of use. From the viewpoint of its effect, as an example, for Corynebacterium and Moraxella bacteria, it is preferably 0.005 w / v% or more, more preferably 0.01 w / v% or more, and even more preferably 0.05 w / v% or more. Furthermore, for Staphylococcus and Pseudomonas bacteria, as an example, it is preferably 0.01 w / v% or more, more preferably 0.025 w / v% or more, and even more preferably 0.05 w / v% or more. For Malassezia, Candida, and Aspergillus fungi, as an example, it is preferably 0.05 w / v% or more, more preferably 0.075 w / v% or more, and even more preferably 0.1 w / v% or more.

[0028] The antibacterial agent of the present invention can be in any form, such as solid, powder, suspension, or foam. However, from the viewpoint of ease of handling, a suspension (slurry) formed by dispersing zinc chloride ore in a dispersion medium is preferred. Water, water-miscible organic solvents, or combinations thereof can be used as the dispersion medium. Examples of water-miscible organic solvents include alcohols such as ethanol or propanol, ethers such as tetrahydrofuran, esters such as methyl acetate, and ketones such as acetone, which are miscible with water. However, from the viewpoint of safety, ethanol is preferred.

[0029] Furthermore, when preparing the antibacterial agent of the present invention into a suspension (slurry), a dispersant is preferably added to prevent zinc chloride ore from agglomerating and precipitating in the suspension, thus becoming difficult to redisperse. Examples of dispersants include ethylene oxide polymers, ethylene oxide-propylene oxide copolymers, cellulose derivatives, or combinations thereof. Specifically, there are no particular limitations, but polyethylene glycol, polypropylene glycol, ethylene oxide-propylene oxide copolymers, hydroxypropyl cellulose, hydroxypropyl methylcellulose, or hydroxyethyl cellulose are preferred. From the viewpoint of further improving the dispersibility of zinc chloride ore in the suspension, hydroxypropyl cellulose, polyethylene glycol 6000, hydroxypropyl methylcellulose, or combinations thereof are more preferred. From the viewpoint of improving dispersibility, the amount of dispersant in the suspension is preferably 0.05% to 10% by mass, more preferably 0.1% to 5% by mass.

[0030] Furthermore, in the antibacterial agent of the present invention, various components may be incorporated in addition to the dispersion medium or dispersant, without impairing the effectiveness of the invention. Examples include surfactants, alcohols, thickeners, preservatives, preservative aids, excipients, fragrances, and pigments.

[0031] Next, a method for manufacturing the antibacterial agent of the present invention will be described. The antibacterial agent may consist solely of zinc chloride ore, but by mixing it with other materials constituting the antibacterial agent, an antibacterial agent containing zinc chloride ore can be obtained. For example, when manufacturing a suspension (slurry) form of antibacterial agent, the antibacterial agent can be obtained by adding zinc chloride ore to a dispersion medium such as water, adding a dispersant as needed, and then dispersing it.

[0032] Furthermore, when manufacturing zinc chloride ore into a suspension (slurry) of nanoparticles as an antibacterial agent, the average particle size (D) of commonly available zinc chloride ore crystals is limited. 50 The ore particles are relatively large, approximately 10 μm in size, therefore requiring micronization to the nanoscale. Wet milling is employed in this micronization process due to its superior performance in achieving nanoscale dimensions. Details are provided below.

[0033] (Mixed steps) First, zinc chloride ore and a dispersion medium are mixed to obtain a suspension. As described above, water, a water-miscible organic solvent, or a combination thereof can be used as the dispersion medium. In this mixing step, it is also preferable to add the aforementioned dispersant so that the suspension contains the dispersant. This is because by including the dispersant in the suspension containing zinc chloride ore and then introducing the suspension into a micronization device to micronize the zinc chloride ore, a slurry with improved dispersibility can be obtained.

[0034] (Stirring step) Next, the suspension obtained by mixing zinc chloride ore and the dispersion medium is stirred. Through this stirring step, the zinc chloride ore in the suspension is fully dispersed (acclimated) in the dispersion medium, thus reducing the likelihood of clogging in the flow path or nozzles of the micronization device during micronization, making the micronization of zinc chloride ore easier. The stirring mechanism is not particularly limited as long as it can stir the entire suspension; for example, a propeller-type stirrer or a magnetic stirrer can be used. To ensure thorough mixing (acclimation) of the dispersion medium and zinc chloride ore, the stirring time is preferably 1 hour or more, more preferably 2 hours or more. Furthermore, it is preferable to add the aforementioned dispersant during this stirring step so that the suspension contains the dispersant. Alternatively, if a water-miscible organic solvent such as ethanol is selected as the dispersion medium, the micronization step can be performed without the stirring step.

[0035] (Micro-processing steps) In the micronization step, the suspension is introduced into a micronization device to micronize the zinc chloride ore in the suspension to nanoscale sizes. A wet micronization device is used as the micronization device, for example, a high-pressure homogenizer type wet micronization device or a bead mill type wet micronization device. The micronization process preferably produces zinc chloride ore with a cumulative particle size (D) of 50%. 50 The process involves using nanoparticles smaller than 500 nm, and more preferably, further fabricating them to achieve a cumulative particle size (D) of 90%. 90 The process involves using nanoparticles smaller than 800 nm. In the micronization process, in the case of a high-pressure homogenizer type device, there are micronization conditions such as the chamber used, pressure, nozzle diameter, number of passes, or processing time. In the case of a bead mill type device, there are micronization conditions such as the material of the beads used, bead diameter, or processing time. However, these processing conditions can be appropriately set according to the specifications of the device used and the desired level of micronization. Alternatively, in this micronization step, a preliminary pulverization treatment can be performed beforehand to pre-crush the zinc chloride ore in the suspension, followed by further micronization. As a preliminary pulverization treatment, the same device as the micronization device described above can be used. Compared to the conditions involved in the micronization treatment itself, the processing time, number of passes, pressure, etc., can be reduced, or the nozzle diameter of the chamber used, the diameter of the beads used, etc., can be increased to pre-crush the zinc chloride ore in the suspension. This allows for a stable 50% cumulative particle size (D) reduction. 50 Small, uniformly sized nanoparticles of zinc chloride ore.

[0036] (Solvent distillation step) Furthermore, when using water-miscible organic solvents such as ethanol as the dispersion medium, the dispersion medium can be replaced with other dispersion media, specifically water, by distilling off the water-miscible organic solvent contained in the micronized water-miscible organic solvent slurry. For example, water is added to the micronized ethanol slurry and the mixture is placed under reduced pressure for steam distillation. This yields an aqueous slurry containing micronized zinc chloride ore after ethanol distillation.

[0037] The slurry obtained through the above-described micronization or distillation steps can be used directly as an antibacterial agent, or in combination with deodorant cosmetics and scalp care cosmetics. Furthermore, the slurry can also be used as an antibacterial agent in the form of a concentrated liquid obtained through concentration treatment, or in a micro-powder form obtained through drying treatment.

[0038] As described above, the antibacterial agent of the present invention has excellent antibacterial effect on resident bacteria of the skin that cause body odor, etc. Therefore, it can be used in conjunction with cosmetics for suppressing body odor (i.e., deodorant) or cosmetics for suppressing scalp odor and preventing dandruff and itching of the scalp.

[0039] The amount of zinc chloride mineral incorporated as an antibacterial ingredient in deodorant cosmetics or scalp care cosmetics according to the present invention can be appropriately set according to the target antibacterial effect, formulation or dosage form, method of use, frequency of use, etc. From the viewpoint of its effect, as an example, relative to Corynebacterium and Moraxella bacteria, it is preferably 0.005 w / v% to 20 w / v, more preferably 0.01 w / v% to 10 w / v, and even more preferably 0.05 w / v% to 5 w / v. Furthermore, for Staphylococcus and Pseudomonas bacteria, as an example, it is preferably 0.01 w / v% to 20 w / v, more preferably 0.025 w / v% to 10 w / v, and even more preferably 0.05 w / v% to 5 w / v. For Malassezia, Candida, and Aspergillus fungi, as an example, the preferred concentration is 0.05 w / v% to 20 w / v, more preferably 0.075 w / v% to 10 w / v, and even more preferably 0.1 w / v% to 5 w / v.

[0040] The deodorant cosmetic or scalp care cosmetic of the present invention, as a dosage form commonly applied to the scalp or skin, can be applied to various topical dosage forms using conventional methods, such as pastes, low-viscosity liquids, gels, emulsions, pastes, creams, solids, powders, foams, ointments, etc. Furthermore, the deodorant cosmetic and scalp care cosmetic of the present invention can also be applied to any of the following: cosmetics, quasi-drugs, or pharmaceuticals. Specific products are not particularly limited, and examples include soaps, cleansers, body lotions, emulsions, gels, creams, talcum powders, skin wipes, stick cosmetics, perfumes, bath products, scalp lotions, shampoos, conditioners, advanced nourishing hair care products, hair growth conditioners, and hair care products.

[0041] Furthermore, in the deodorant cosmetics or scalp care cosmetics of the present invention, various ingredients commonly used in cosmetics or topical agents may be incorporated without impairing the effectiveness of the present invention. Examples include antiperspirants, deodorants, alcohols, surfactants, thickeners, vitamins, amino acids, moisturizers, preservatives, preservative aids, fragrances, and pigments. Additionally, antibacterial ingredients other than zinc chloride may be incorporated. Other antibacterial ingredients include, for example, phenoxyethanol, isopropyl methylphenol, or benzalkonium chloride.

[0042] In addition, as described above, the antibacterial agent of the present invention has antibacterial effects against bacteria of the genus Corynebacterium, bacteria of the genus Moraxella, or bacteria of the genus Malassezia, as well as bacteria of the genus Staphylococcus, bacteria of the genus Pseudomonas, fungi of the genus Candida, or fungi of the genus Aspergillus. Therefore, by including this antibacterial agent in various products, antibacterial products with antibacterial effects against the above-mentioned microorganisms can be easily obtained.

[0043] As antibacterial products, paper or cloth can be cited as examples. For products where antibacterial properties are desired, zinc chloride ore can be incorporated through methods such as addition, impregnation, dispersing, or coating to produce antibacterial products. For instance, by impregnating paper or cloth in an antibacterial agent composed of a zinc chloride ore slurry and then drying it, antibacterial paper or cloth products can be obtained. Furthermore, cloth includes not only woven fabrics but also non-woven fabrics. As an example, the concentration of zinc chloride ore in the zinc chloride ore slurry used to impregnate the target antibacterial product can be appropriately set according to the desired antibacterial effect, control products, etc., preferably 0.05 w / v% or more, and more preferably 0.1 w / v% or more.

[0044] The invention will now be described in more detail by way of examples and comparative examples, but the invention is not limited by these examples.

[0045] Example [Example 1] 1. Study on the antibacterial effect of chlorinated zinc ore (1) In this embodiment, the antibacterial activity of zinc chloride ore against Corynebacterium, Moraxella, and Malassezia bacteria, which are microorganisms that cause body odor, scalp odor, and stuffy odor, was investigated.

[0046] [Preparation of aqueous slurry sample a of zinc chloride ore nanoparticles] 440g of zinc chloride ore (JFE Mineral Co., Ltd.; batch number: S1J201) was added to 1672g of ion-exchanged water to obtain a suspension. The suspension was stirred at 500-1000 rpm for 3 hours using a propeller mixer, and then allowed to stand overnight. The aqueous slurry of the zinc chloride ore (aqueous suspension) was introduced into a high-pressure homogenizer-type wet micronization device (STARBURST LABO; SUGINO Co., Ltd.) for pre-micronization under the following conditions: chamber type: slit-type chamber; chamber nozzle diameter: 0.16mm; pressure: 150MPa; number of passes: 5. After pre-micronization, the chamber of the wet micronization device was changed to another slit-type chamber (model: ESC-101; chamber nozzle diameter: less than 0.16 mm). Micronization was carried out under a pressure of 150 MPa and 50 passes, resulting in an aqueous slurry sample a (zinc hydrate mineral concentration: 21 w / w%) of zinc hydrate mineral particles micronized to nanoscale size. The particle size distribution of zinc hydrate mineral in this aqueous slurry was measured, and the results showed that 50% of the cumulative particle size (D) was... 50 The particle size is 160 nm, and the 90% cumulative particle size (D) is... 90 The wavelength is 478nm.

[0047] Furthermore, the particle size distribution of zinc chloride ore was determined using a laser diffraction scattering particle size distribution measuring device (model: Partica LA-960; product of Horiba Manufacturing Co., Ltd.). The refractive index parameter of the zinc chloride ore crystals was set to 1.700-0.000i, and ion-exchanged water containing zinc chloride ore was used as the dispersion medium. Based on the obtained particle size distribution, the 50% cumulative particle size (D) in the volumetric particle size distribution was calculated. 50 ) and 90% cumulative particle size (D 90 ).

[0048] [Antibacterial Activity Evaluation Test] The antimicrobial activity of zinc chloride ore was evaluated using the test microorganisms and culture media shown below.

[0049] <Test Microorganisms> Corynebacterium bandingense ( Corynebacterium striatum NBRC15291 Corynebacterium jejuni ( Corynebacterium jeikeium JCM9384 ·Moraxella osloensis ( Moraxella osloensis )NBRC113899 Malassezia furfur ( Malassezia furfur NBRC10987 <Culture medium> Culture medium A: Mueller-Hinton agar medium {Mueller-Hinton II broth, ion-prepared (BDI) and 1.5% agar powder (Fujifilm and Kazumitsu Pure Chemical Industries, Ltd.)} Culture medium B: Columbia agar medium supplemented with 5% defibrinated sheep blood {Devco Columbia Blood Agar Basal Medium (BD Biosciences product) and 5% defibrinated sheep blood (Cosmo Biosciences product)} Culture medium C: CHROMagar Malassezia / Candida ( Malassezia / Candida Culture medium (Kanto Chemical Co., Ltd. product) The test microorganisms stored at -70°C were inoculated into agar medium and incubated at 32°C for 24–72 hours to allow them to recover. Specifically, *Moraxella* was recovered using medium A (Mueller-Hinton agar), both *Corynebacterium* species were recovered using medium B (Columbia agar supplemented with 5% defibrinated sheep blood), and *Malassezia* was recovered using medium C (CHROMagar Malassezia / Candida agar). Colonies of the grown test microorganisms were collected and suspended in sterile physiological saline (Otsuka Pharmaceutical Co., Ltd. product) to prepare a turbidity equivalent to McFarlane No. 0.5 (approximately 10...). 8 The bacterial suspension (CFU / mL) was prepared by serially diluting the bacterial suspension 10 times with sterile physiological saline.

[0050] On the other hand, when preparing culture media A and B for antibacterial activity evaluation tests, each culture medium was prepared, sterilized by heating, and then cooled to approximately 60°C. A specified amount of the aqueous slurry sample a (specific gravity 1.20) of zinc chloride nanoparticles prepared above was added and mixed into each culture medium to prepare agar plate culture media with concentrations of 0.025 w / v% and 0.050 w / v% of zinc chloride nanoparticles. Furthermore, when preparing culture medium C for antibacterial activity evaluation tests, since the culture medium was already sold in its agar plate form, a diluted slurry of the aqueous slurry sample a of zinc chloride nanoparticles prepared above was prepared by appropriately diluting it with sterile water. This diluted slurry was then spread onto agar medium to prepare culture media C with concentrations of 0.13 w / v% and 0.25 w / v% of zinc chloride nanoparticles. Additionally, for comparison, culture media without zinc chloride nanoparticles (0 w / v%) were also prepared.

[0051] On the culture medium used for antimicrobial activity evaluation tests, approximately 0.1 mL of the serially diluted inoculum solution prepared as described above was spread onto each medium and incubated at 32°C for 24–72 hours. Additionally, as shown in Table 1 below, for *Corynebacterium* spp., *Corynebacterium striatum*, the resuscitation medium was medium B (Columbia agar medium supplemented with 5% defibrinated sheep blood), and the antimicrobial activity evaluation tests were conducted using both medium A (Mueller-Hinton agar medium) and medium B. After incubation, the number of viable bacteria in the medium containing *Zincium chloride* nanoparticles was counted, based on the dilution of the inoculum solution spread at a dilution factor where the viable bacterial count could be confirmed as tens to hundreds in a medium without *Zincium chloride* nanoparticles. The results are shown in Table 1 below.

[0052] [Table 1]

[0053] The results showed that zinc chloride ore exhibited excellent antibacterial activity against microorganisms of the genera *Moraxella*, *Corynebacterium*, and *Malassezia*. Furthermore, zinc chloride ore inhibited the growth of *Moraxella* and *Corynebacterium* bacteria by at least 0.025 w / v%, and inhibited the growth of *Malassezia* fungi by at least 0.13 w / v.

[0054] [Example 2] 2. Study on the antibacterial effect of chlorinated zinc ore (2) In this embodiment, based on the results of Example 1 above, the minimum inhibitory concentration (MIC) against Corynebacterium spp., Moraxella spp., and Staphylococcus spp. was measured using the micro-liquid dilution method. Additionally, Staphylococcus aureus (Staphylococcus aureus) was used as a Staphylococcus spp. Staphylococcus aureusIt is also a type of resident bacteria on the skin. Therefore, in this embodiment, in the evaluation of antibacterial activity, a variety of samples were prepared and tested: samples with different particle sizes of zinc chloride ore, samples combined with a dispersant, and samples containing heterogeneous zinc chloride ore.

[0055] [Preparation of aqueous slurry sample b from zinc chloride ore] 16g of pure water was added to 4g of zinc chloride ore (JFE Mineral Co., Ltd.; batch number: S1J201), and the mixture was stirred for 5 minutes using a vortex mixer to obtain a slurry sample b of zinc chloride ore (zinc chloride ore concentration: 20w / w%). The particle size distribution of zinc chloride ore in this slurry was measured, and the results showed that 50% of the cumulative particle size (D) was... 50 The particle size is 13700 nm (13.7 μm) with a 90% cumulative particle size (D). 90 The wavelength is 144000nm (144μm).

[0056] [Preparation of aqueous slurry sample c containing dispersant for zinc chloride ore nanoparticles] 56g of polyethylene glycol 6000 (PEG-6000P, a product of Sanyo Chemical Industry Co., Ltd.) was dissolved as a dispersant in 1043g of ion-exchanged water. 280g of zinc chloride ore (JFE Mineral Co., Ltd.; batch number: S1J201) was added to this solution to obtain a suspension. The suspension was stirred at 500-1000 rpm for 2 hours using a propeller mixer, and then allowed to stand overnight. The zinc chloride ore slurry (aqueous suspension) was treated under the same conditions and methods as the pre-micronization and micronization treatment of the aqueous slurry sample a prepared in Example 1, except that the number of passes in the micronization process was set to 40, to obtain a PEG-containing aqueous slurry sample c (zinc chloride ore concentration: 20.3 w / w%) with zinc chloride ore particles micronized to nanoscale sizes. The particle size distribution of the zinc chloride ore in this aqueous slurry was measured, and it was found that the cumulative particle size (D) was 50%. 50 The particle size is 206 nm, and the 90% cumulative particle size (D) is... 90 The wavelength is 444nm.

[0057] Preparation of aqueous slurry sample d containing dispersant of zinc chloride ore nanoparticles 34.8 g of hydroxypropyl cellulose (HPC-L, Nippon Soda Co., Ltd.) was dissolved as a dispersant in 1531.2 g of ion-exchanged water. 174 g of zinc chloride ore (JFE Mineral Co., Ltd.; batch number: S1J201) was added to this solution to obtain a suspension. The suspension was stirred at 500-1000 rpm for 2 hours using a propeller mixer, and then allowed to stand overnight. The zinc chloride ore slurry (aqueous suspension) was treated under the same conditions and methods as the pre-micronization and micronization treatment of the aqueous slurry sample a prepared in Example 1 to obtain a slurry sample d containing HPC containing zinc chloride ore nanoparticles with micronized zinc chloride ore particles at the nanoscale (zinc chloride ore concentration: 10 w / w%). The particle size distribution of zinc chloride ore in the aqueous slurry was measured, and the results showed that the 50% cumulative particle size (D) was... 50 The particle size is 152 nm, and the 90% cumulative particle size (D) is... 90 The wavelength is 730nm.

[0058] [Preparation of aqueous slurry sample e containing heterogeneous zinc chloride ore nanoparticles and dispersant] The Japanese Pharmacopoeia describes a process where 40g of polyethylene glycol 6000 (PEG-6000P, a product of Sanyo Chemical Industry Co., Ltd.) was dissolved as a dispersant in 1600g of anhydrous ethanol. Then, 400g of zinc chloride ore (JFE Mineral Co., Ltd., a product containing heterogeneous phases) was added to this solution with stirring to obtain a suspension. This suspension was then introduced into a wet micronization device (MAX Nano & Getter HFM06, a product of Ashizawa Finetech Co., Ltd.). After pulverizing with 0.05mm diameter PSZ beads for 40 minutes, 485g of anhydrous ethanol was added, followed by pulverization for another 10 minutes, thus performing micronization. This yielded an ethanol slurry with a zinc chloride ore concentration of 15.8 w / w%. The particle size distribution of the zinc chloride ore in this ethanol slurry was measured, and the results showed that the cumulative particle size (D) was 50%. 50 The particle size is 136 nm, and the 90% cumulative particle size (D) is... 90 The wavelength was 907 nm. 39 g of pure water was added to 25 g of the ethanol slurry. After distilling off the ethanol by steam distillation under reduced pressure, pure water was added to make the total mass 39 g, resulting in an aqueous slurry sample e containing heterogeneous zinc chloride mineral nanoparticles and a dispersant (zinc chloride mineral concentration: 10 w / w%).

[0059] [Measurement of Minimum Inhibitory Concentration (MIC)] MIC was measured using the test microorganisms and culture media shown below. MIC measurements were performed with reference to the journal Chemotherapy, 『MIC determination method for microfluidics (microfluidics method)』, Vol.38, No.1, pp.103-105.

[0060] <Test Microorganisms> Staphylococcus aureus ( Staphylococcus aureus NCTC10788 Corynebacterium bandingense ( Corynebacterium striatum NBRC15291 Corynebacterium jejuni ( Corynebacterium jeikeium JCM9384 ·Moraxella osloensis ( Moraxella osloensis )NBRC113899 <Culture medium> Resuscitation medium A: Mueller-Hinton agar medium {Mueller-Hinton II broth, ion-prepared (BDI) and 1.5% agar powder (Fujifilm and Kazumitsu Chemical Co., Ltd., Japan)} Resuscitation medium B: Columbia agar medium supplemented with 5% defibrinated sheep blood {Devco Columbia Blood Agar Basal Medium (BD Biosciences product) and 5% defibrinated sheep blood (Cosmo Biosciences product)} Experimental medium D: Mueller-Hinton liquid medium {Far East Mueller-Hinton broth (product of Far East Pharmaceutical Industries Co., Ltd.)} Experimental culture medium E: Mueller-Hinton broth supplemented with equine hemolytic disease {Far East equine hemolytic disease plus Mueller-Hinton broth (Far East Pharmaceutical Industries Co., Ltd. product)} Test microorganisms stored at -70°C were inoculated into resuscitation medium and incubated at 32°C for 18–48 hours to allow for resuscitation. Staphylococcus aureus and Moraxella catarrhalis were resuscitated using medium A (Mueller-Hinton agar), while the two Corynebacterium species were resuscitated using medium B (Columbia agar supplemented with 5% defibrinated sheep blood). Colonies of the grown test microorganisms were collected and suspended in sterile physiological saline (Otsuka Pharmaceutical Co., Ltd.) to prepare a turbidity equivalent to McFarlane No. 0.5 (approximately 10). 8 The bacterial suspension (CFU / mL) was diluted 10-fold (approximately 10 CFU / mL) with sterile physiological saline. 7 (CFU / mL) to prepare inoculation solution.

[0061] In preparing test media D and test media E for MIC measurement, aqueous slurry samples a-e of zinc chloride ore prepared in Example 1 and above were prepared using pure water to achieve a zinc chloride ore concentration of 10 w / w% (specific gravity 1.06). Then, media were prepared by diluting each medium approximately 10 times (zinc chloride ore concentration: 11000 μg / mL). These were then diluted sequentially with the media to prepare test media D and test media E in a 2-fold dilution series. Additionally, for comparison, test media without zinc chloride ore (zinc chloride ore concentration: 0 μg / mL) were also prepared.

[0062] The experiment used U-bottom microplates (IWAKI microplates, 96 wells, capped, AGC TECHNO GLASS). Approximately 0.1 mL of a series of test media containing 11 μg / mL to 11000 μg / mL zinc chloride ore and 0.005 mL of the inoculum prepared as described above were added to each well, and the plates were incubated at 32°C for 18–48 hours. As shown in Table 2 below, *Staphylococcus aureus* was tested in test medium D, *Corynebacterium jejuni* was tested in test medium E, and *Corynebacterium striatum* and *Moraxella osloensis* were tested in both test media D and test medium E. After incubation, the minimum concentration of zinc chloride ore in the well where bacterial growth was not visible to the naked eye was used as the MIC after confirming bacterial growth in the control medium without zinc chloride ore. Here, when the amount of zinc chloride mineral in the test medium increases, the zinc chloride mineral itself will cause changes in turbidity. Therefore, wells are set up where only the test medium is added without adding the inoculum to ensure that the turbidity is not affected by the zinc chloride mineral when determining the MIC of the zinc chloride mineral.

[0063] In addition, based on the obtained MIC, the antibacterial effect was evaluated based on the following benchmarks.

[0064] "+++" (has good antibacterial effect): MIC is less than 200 μg / mL.

[0065] "++" (with antibacterial effect): MIC is above 200 μg / mL and less than 1000 μg / mL.

[0066] "+" (has a certain antibacterial effect): MIC is above 1000μg / mL and less than 5000μg / mL.

[0067] "-" (no antibacterial effect): MIC is above 5000 μg / mL.

[0068] The results are shown in Table 2 below. The markings in parentheses in each column indicate the evaluation results of the antibacterial effect.

[0069] [Table 2]

[0070] These results demonstrate that zinc chloride ore exhibits excellent antibacterial effects against Staphylococcus aureus, Corynebacterium spp., and Moraxella spp. There was no significant difference in antibacterial efficacy among the added samples a–e, but for Staphylococcus aureus, the antibacterial effect was improved by preparing zinc chloride ore into nanoparticles. Furthermore, even with the addition of dispersants that enhance the dispersibility of zinc chloride ore, the antibacterial effect remained the same compared to samples without dispersants. Additionally, no difference in excellent antibacterial efficacy was observed even when zinc chloride ore contained heterogeneous phases.

[0071] In addition, although not recorded in Table 2, sample solutions containing 2% by mass were prepared for PEG6000 in samples c and e as dispersants and HPC (hydroxypropyl cellulose) in sample d. After being diluted 10 times with test culture medium, MIC was determined based on a 2-fold dilution series. However, no antibacterial activity was found in PEG6000 and HPC.

[0072] [Comparative Example 1] 3. Study on the antibacterial effect of supernatant from chlorinated zinc ore slurry Zinc hydrate ore is insoluble in water, but it is said that trace amounts of zinc ions dissolve in water. Therefore, in this comparative example, to investigate whether zinc hydrate ore exhibits an antibacterial effect through the dissolution of zinc ions into the aqueous slurry of zinc hydrate ore, a supernatant of the aqueous slurry was prepared, and the supernatant was serially diluted and the MIC was determined. Specifically, aqueous slurry samples a-c of zinc hydrate ore obtained in Examples 1 and 2 were prepared with pure water to a zinc hydrate ore concentration of 10 w / w, and then centrifuged at 1830 × g for 30 minutes (model: AX-521; TOMY Seiko Co., Ltd.). The supernatant was recovered and filtered through a 0.22 μm membrane filter (Millex GV filter; Merk Co., Ltd.), and a colorless and clear filtrate was obtained as the supernatant of aqueous slurry samples a-c with a zinc hydrate ore concentration of 10 w / w.

[0073] Using the same test microorganisms and culture media as in Example 2, the minimum inhibitory concentration (MIC) was determined in the supernatants of aqueous slurry samples a-c with a zinc chloride ore concentration of 10 w / w, as described above. In preparing test media D and test media E for MIC determination, the supernatants of aqueous slurry samples a-c with a zinc chloride ore concentration of 10 w / w were diluted with each culture medium to prepare a culture medium containing the supernatant of the aqueous slurry with a zinc chloride ore concentration equivalent to 11000 μg / mL. This was then sequentially diluted with culture media to prepare test media D and test media E in a 2-fold dilution series. Additionally, test media without supernatant were prepared as a comparative control.

[0074] U-bottom microplates were used in the experiment. Using a 2-fold dilution series, approximately 0.1 mL of test medium containing zinc chloride ore at a concentration equivalent to 11 μg / mL to 11000 μg / mL of aqueous slurry supernatant and test medium without aqueous slurry supernatant were added to each well. Approximately 0.005 mL of inoculum prepared in the same manner as in Example 2 was added to each well, and the plates were incubated at 32°C for 18–48 hours. After incubation, the bacterial growth in the medium without aqueous slurry supernatant (used as a control) was confirmed. The minimum concentration of zinc chloride ore in the aqueous slurry corresponding to the aqueous slurry supernatant in the wells where no bacterial growth was visible to the naked eye was taken as the MIC. That is, the MIC in this comparative example represents the zinc chloride ore concentration of the aqueous slurry obtained from its supernatant. Furthermore, based on the determined MIC, the antibacterial effect was evaluated in the same manner as in Example 2. The results are shown in Table 3 below.

[0075] [Table 3]

[0076] The results indicate that zinc ions exhibiting antibacterial effects against the tested microorganisms were absent in the supernatant of the zinc chloride ore slurry, and there was no antibacterial effect whatsoever. This demonstrates that the excellent antibacterial effect of the zinc chloride ore slurry samples shown in Examples 1 and 2 was not caused by free zinc ions in the slurry, but rather by the zinc chloride ore itself. In other words, the test results of Comparative Example 1 and Example 2 show that "zinc chloride ore itself" possesses antibacterial activity.

[0077] [Example 3] 4. Study on the antibacterial effect of chlorinated zinc ore (3) In this embodiment, as a microbial species other than those previously studied for their antibacterial effects, Pseudomonas aeruginosa: Pseudomonas aeruginosa (… Pseudomonas aeruginosa Candida: Candida albicans ( Candida albicans Aspergillus: Aspergillus brasiliensis ( Aspergillus brasiliensis Staphylococcus aureus, whose antibacterial effects were also studied in Example 2, and Staphylococcus aureus (…). Staphylococcus aureusThe antibacterial activity of zinc chloride ore was studied. Specifically, the experiment was conducted as follows: The aqueous slurry sample a of zinc chloride ore prepared in Example 1 above was diluted with sterile water to prepare a slurry with a zinc chloride ore concentration of 0.1 w / v%, which was filled into a container. The container was then inoculated with the microorganisms shown in Table 4 below to achieve the viable count shown in Table 4, and the mixture was uniformly mixed.

[0078] [Table 4]

[0079] These samples were stored at 22.5 ± 2.5 °C for 28 days under light-protected conditions, and viable bacterial counts were determined on days 0, 14, and 28. The results from day 28 were used for interpretation, and the percentage of bacteria count at the start of the experiment was calculated as 100. The interpretation criteria followed the criteria (Category IB) specified in the Japanese Pharmacopoeia. Furthermore, for Candida, as an important indicator of antibacterial activity, the same criteria as for bacteria were applied. The labeling method for the viable bacterial count results is shown in Table 5, and the viable bacterial count results and interpretation results are shown in Table 6.

[0080] [Table 5]

[0081] [Table 6]

[0082] According to the results, zinc chloride ore has antibacterial effects against Pseudomonas, Staphylococcus, Candida, and Aspergillus bacteria, indicating that it has antibacterial effects not only against previously studied bacteria, but also against fungi such as Candida and Aspergillus.

[0083] [Example 4] 5. Antibacterial effect of chlorine-containing zinc ore processing products In this embodiment, the antibacterial effects of paper and cloth impregnated with zinc chloride ore were studied. Filter paper (NO.5B, ADVANTEC Toyo Co., Ltd.) and a one-dollar bill were selected as the "paper" for processing with zinc chloride ore. The paper was cut into 4cm square pieces and then autoclaved at 121°C for 20 minutes. As for the "cloth" for processing with zinc chloride ore, cellulose nonwoven fabric (BEMCOT (registered trademark) M-3II; Asahi Kasei Corporation) was selected. The cloth was cut into pieces of approximately 0.4g and then autoclaved at 121°C for 20 minutes.

[0084] The aqueous slurry sample a of zinc chloride ore adjusted in Example 1 above was diluted with physiological saline to prepare test solutions with zinc chloride ore concentrations of 0.05 w / v%, 0.13 w / v%, and 0.5 w / v, respectively. Sterilized paper and cloth pieces were immersed in these test solutions and physiological saline for at least 1 minute, then removed, excess liquid was removed, and the pieces were air-dried on a clean bench for approximately 30 minutes. Additionally, for the $1 banknote in the paper piece, only tests related to the 0.5 w / v% test solution were performed.

[0085] On the other hand, Staphylococcus aureus (Staphylococcus aureus) was used as the inoculation bacteria. Staphylococcus aureus NCTC10788 / ATCC6538: BIOBALL (registered trademark) products of BioMérieux. (To approximately 10...) 8 Add 1 mL of the rehydration solution provided with the kit to each frozen Staphylococcus aureus cell, mix thoroughly, and then dilute with Nutrient Broth (Solabia Biokar Diagnostics) to prepare approximately 2 × 10⁻⁶ cells. 5 / mL. To confirm the viable count, appropriate dilutions were performed and plated onto Mueller-Hinton agar medium (Mueller-Hinton II liquid medium, ion preparation (BDIC product)) and 1.5% agar powder (Fujifilm and Koko Pure Chemical Industries, Ltd., Japan) for incubation, confirming a viable count of 1~3×10⁻⁶. 5 Within the range of CFU / mL (2.7×10⁻⁶) 5 (CFU / mL).

[0086] For the air-dried paper and cloth pieces, use a micropipette to transfer Staphylococcus aureus (2.7 × 10⁻⁶) to the container. 50.2 mL of inoculum (CFU / mL) was aliquoted into multiple sites and placed in 50 mL conical tubes. As a 0-hour sample immediately after inoculation, 20 mL of physiological saline was immediately added and vigorously stirred for elution. 1 mL of the eluent was serially diluted in Mueller-Hinton liquid medium. 1 mL of each dilution was then mixed with 1 mL of soft agar (prepared with 1.2% Mueller-Hinton agar·ionomer, sterilized and stored in a water bath at 40-50°C). A layer was spread onto Mueller-Hinton agar, and after the medium solidified, the tubes were incubated in a constant temperature bath (35°C) for 24 hours, and the number of bacteria grown was measured. Alternatively, as a 20-hour sample, a paper or cloth disc inoculated with Staphylococcus aureus was placed in a 50 mL conical tube, and the opening was covered with paraffin film. The tube was then incubated in a constant temperature bath at 35°C for approximately 20 hours. After 20 hours, 20 mL of physiological saline was added and vigorously stirred for elution. For this eluent, the number of bacteria grown was measured by the mixed dilution plate culture method using the same materials and methods as for the eluent of the 0-hour sample. Additionally, the results for tests related to filter paper and nonwoven fabric were confirmed to be the average of 3 cases, and the results for tests related to banknotes were confirmed to be the average of 2 cases, with the differences between individual test groups within 10 times.

[0087] In addition, the antibacterial effect is evaluated according to the following criteria based on the measured viable bacterial count. Furthermore, "proliferating bacterial count" refers to the number of viable bacteria obtained by subtracting the number of viable bacteria immediately after inoculation from the number of viable bacteria after 20 hours of culture, as commonly used as the logarithmic value shown in Table 7 below.

[0088] "◎" (has excellent antibacterial effect): (common logarithmic value of the number of proliferating bacteria in the control area) - (average value of the common logarithmic value of the number of proliferating bacteria in the test area) is 3 or more.

[0089] "○" (has antibacterial effect): (common logarithmic value of the number of proliferating bacteria in the control area) - (average value of the common logarithmic value of the number of proliferating bacteria in the test area) is greater than 2 and less than 3.

[0090] "×" (no antibacterial effect): (common logarithmic value of bacterial count in the control area) - (average value of common logarithmic value of bacterial count in the test area) is less than 2.

[0091] The results are shown in Table 7 and Figure 1 below.

[0092] Table 7

[0093] According to these results, antibacterial paper or fabric products can be obtained simply by impregnating paper or fabric in a slurry containing zinc chloride ore and then drying it. Furthermore, even a low concentration of zinc chloride ore in the impregnation solution (0.05 w / v%) imparts an antibacterial effect (especially for fabric products), and the higher the concentration of zinc chloride ore in the impregnation solution, the higher the antibacterial activity (especially for paper products). This indicates that antibacterial products can be easily obtained by incorporating (adhering to) zinc chloride ore into paper or fabric products.

[0094] [Example 5] 6. Preparation of deodorant cosmetics The aqueous slurry sample a of chlorinated zinc ore nanoparticles obtained in Example 1 contained 10.0% by mass, dipropylene glycol 10% by mass, propylene glycol-10-methylglucose 2% by mass, butylene glycol 20% by mass, diglyceryl ascorbic acid 1% by mass, 1,2-hexanediol 1% by mass, glycine 1% by mass, arginine 1% by mass, glycerol 0.5% by mass, hydroxyethyl cellulose 0.5% by mass, dextran 0.5% by mass, hydroxypropyl cyclodextrin 2% by mass, grapefruit fruit extract 0.2% by mass, red ginseng root extract 0.2% by mass, and acetyl tetrapeptide-3. 0.2% by weight, 0.2% by weight of safflower clover extract, 0.2% by weight of biotin, 0.2% by weight of riboflavin, 0.2% by weight of pyridoxine hydrochloride, 0.2% by weight of dipotassium glycyrrhizate, 0.2% by weight of acetylated sodium hyaluronate, 0.2% by weight of hydrolyzed elastin, 0.2% by weight of cholesterol, 0.2% by weight of PEG-40 hydrogenated castor oil, 0.2% by weight of polyglycerol-10 myristate, 0.2% by weight of diphenylpolydimethylsiloxane, 0.05% by weight of Bergamot fruit oil, 0.05% by weight of neroli oil The following ingredients are mixed: oil, lime juice, orange juice, lemon juice, hawthorn extract, jujube fruit extract, grapefruit fruit extract, apple fruit extract, and the remainder water to obtain a slightly viscous, light peach-white suspension for deodorizing cosmetics (the content of zinc chloride minerals in the lotion is 2.0% by mass).

[0095] [Example 6] 7. Preparation of cosmetics (shampoos) for the prevention and improvement of scalp dandruff, itching, or scalp odor. The aqueous slurry sample a of zinc chloride ore nanoparticles obtained in Example 1 contained 0.50% by mass, sodium lauroyl methyl alanine 2% by mass, cocamidopropyl betaine 2% by mass, cocamidopropyl MEA 0.5% by mass, glycerol 10% by mass, 1,2-hexanediol 0.5% by mass, citric acid 1% by mass, lauryl glucoside 2% by mass, bergamot fruit oil 0.05% by mass, and polyquaternium-10 0.02% by mass, menthol 0.2% by mass, hydroxypropyl cyclodextrin 1.3% by mass, arginine 1% by mass, dipropylene glycol 10% by mass, kaolin 2% by mass, glycine 1% by mass, dipotassium glycyrrhizate 0.8% by mass, panthenol 1% by mass, biotin 0.1% by mass, riboflavin 0.1% by mass, hydrolyzed keratin 0.1% by mass, hydrolyzed sodium hyaluronate 0.1% by mass, shea butter 2% by mass, hydrogenated lecithin 0.2% by mass, 1,3-butanediol 20% by mass, cocoyl glutamate triethanolamine salt 3% by mass The following ingredients were mixed: 0.2% by mass of hydrolyzed keratin (wool), 1% by mass of sodium lactate, 0.1% by mass of phytosterols, 0.1% by mass of N-stearoyl dihydrosphingosine, 2% by mass of sodium dodecylaminopropionate, 0.1% by mass of sodium citrate, 1% by mass of sodium chloride, 0.2% by mass of persimmon tannin, 0.1% by mass of lactic acid, 0.5% by mass of pentylene glycol, 0.1% by mass of tocopherol, 0.5% by mass of tannic acid, and the balance of water to obtain a slightly viscous scalp cleansing shampoo with good foaming properties (the content of the component as zinc chloride mineral in the shampoo is 0.1% by mass).

[0096] This invention is not limited to the above-described embodiments or examples, and various design changes that do not depart from the spirit of the invention as set forth in the claims are also included within the scope of the invention.

[0097] Industrial availability The antibacterial agents, deodorants, cosmetics, and cosmetics and antibacterial products for the prevention and improvement of dandruff, itching, or scalp odor of the present invention have excellent antibacterial effects and are therefore widely used not only in the field of beauty and hair care but also in other fields.

Claims

1. An antibacterial agent, characterized in that, Contains zinc ore with chlorine water The antibacterial agent is effective against at least one resident skin bacterium selected from the group consisting of Corynebacterium, Moraxella, and Malassezia.

2. The antibacterial agent according to claim 1, characterized in that, The zinc ore in the chlorine water is in the form of nanoparticles.

3. The antibacterial agent according to claim 1, characterized in that, The chlorinated zinc ore is based on the 50% cumulative particle size (D) in the volume-based particle size distribution obtained by laser diffraction scattering. 50 ) refers to nanoparticles smaller than 500nm.

4. A deodorant cosmetic, characterized in that, Contains any one of the antibacterial agents according to claims 1 to 3.

5. A cosmetic product for preventing and improving scalp dandruff, itching, or scalp odor, characterized in that, Contains any one of the antibacterial agents according to claims 1 to 3.

6. An antibacterial agent, characterized in that, Contains zinc ore with chlorine water The antimicrobial agent is an antimicrobial agent targeting at least one microorganism selected from the group consisting of Corynebacterium, Moraxella, Malassezia, Staphylococcus, Pseudomonas, Candida, and Aspergillus.

7. An antibacterial product, characterized in that, It contains the antibacterial agent as described in claim 6.

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