Reducing and synergistic bactericide and application thereof in prevention and treatment of black spot of oilseed rape

Abstract

The invention discloses a decrement and synergism bactericide and application thereof in prevention and treatment of black spot of rape, and belongs to the technical field of bactericides. According to the invention, by combining the advantages of high efficiency and rapid sterilization effect of the organic bactericide and high safety, difficult generation of drug resistance and biodegradability of the natural bactericide, not only is an excellent prevention and treatment effect achieved, but also the dosage of the organic bactericide is reduced; 3, 6-disubstituted imidazole [1, 2-b] pyridazine derivatives and phenazine alpha-2-carboxylic acid are selected as the organic bactericides, and the 3, 6-disubstituted imidazole [1, 2-b] pyridazine derivatives and the phenazine alpha-2-carboxylic acid have a good inhibition effect on black spot germs; the natural bactericide is selected from garlic, bamboo leaves, fennel and turmeric, contains active ingredients with an inhibiting effect on germs, and is matched with the organic bactericide for use, so that an excellent prevention and treatment effect is achieved; by combining with the synergist, the tension of the bactericide on the plant surface is effectively reduced, the utilization rate of effective components is improved, and the dosage of the bactericide is reduced.

Description

Technical Field

[0001] This invention belongs to the field of fungicide technology, and particularly relates to a fungicide that reduces dosage and increases efficacy and its application in the prevention and control of rapeseed black spot disease. Background Technology

[0002] Black spot disease is a common foliar disease in rapeseed growing areas, mainly affecting the leaves, petioles, stems, and pods. The pathogen overwinters primarily on seeds, in the soil, and on diseased plant debris, surviving in the soil for over a year. It can infect at any time, and is more likely to occur after rain. Infected leaves initially develop small dark green spots, which later turn light black to dark brown. Some spots extend along the veins, and several spots often merge into large, irregular necrotic patches. Severe cases can lead to browning of the veins, yellowing and leaf drop, or twisting and deformation of the leaves. Infected stems and tassels develop irregular, dark brown streaks, while pods develop sunken, irregular brown papules.

[0003] Common control methods for rapeseed black spot disease include agricultural control, seed treatment, and chemical control. Chemical control is widely used due to its relatively low cost and ability to achieve significant control effects in a short period. However, long-term use of existing chemical fungicides can easily increase disease resistance, leading to increased pesticide dosages and reduced subsequent control effectiveness.

[0004] Therefore, how to provide a fungicide that requires a small dosage and can effectively prevent and control rapeseed black spot disease has become a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a fungicide with reduced dosage and enhanced efficacy, and its application in the control of rapeseed black spot disease. The fungicide provided by this invention can maintain control efficacy while reducing dosage, offering the advantages of low dosage and high control effect.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention provides a fungicide with reduced dosage and increased efficacy, the fungicide comprising an organic fungicide, a natural fungicide, and a synergist; the mass ratio of the organic fungicide, the natural fungicide, and the synergist is (8-15):(15-30):(0.5-2);

[0008] The organic bactericides include 3,6-disubstituted imidazole[1,2-b]pyridazine derivatives and phenazine α-2-carboxylic acid;

[0009] The natural bactericides include garlic, bamboo leaves, fennel, and mint.

[0010] Technical Principle: This invention combines organic and natural fungicides, integrating the high efficiency and rapid bactericidal effect of organic fungicides with the advantages of natural fungicides such as high safety, low resistance, and biodegradability. This achieves excellent control while reducing the dosage of organic fungicides. The organic fungicides selected are 3,6-disubstituted imidazole [1,2-b]pyridazine derivatives and phenazine α-2-carboxylic acid, both of which have good inhibitory effects on black spot pathogens and exhibit good persistence and broad spectrum in field applications. The natural fungicides selected are garlic, bamboo leaves, fennel, and mint, which contain active ingredients that inhibit pathogens. When used in combination with organic fungicides, they achieve excellent control effects. Furthermore, the addition of synergists effectively reduces the surface tension of the fungicide on plants, improves the utilization rate of active ingredients, and reduces the dosage of fungicide, resulting in a fungicide with low dosage and good control effect.

[0011] Furthermore, the mass ratio of the 3,6-disubstituted imidazole[1,2-b]pyridazine derivative to phenazine α-2-carboxylic acid is (5-10):(1-3).

[0012] Furthermore, the mass ratio of garlic, bamboo leaves, fennel, and mint is (3-8):(2-5):(1-4):(1-4).

[0013] Furthermore, the synergist includes curcumin and matrine.

[0014] Furthermore, the mass ratio of curcumin to matrine is (3-6):(2-4).

[0015] Furthermore, the 3,6-disubstituted imidazo[1,2-b]pyridazine derivatives are selected from one or more of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, 3,6-diphenylimidazo[1,2-b]pyridazine, 6-chloro-3-(2-bromophenyl)imidazo[1,2-b]pyridazine, and 3-(benzo[b]thiophen-2-yl)-6-chloroimidazo[1,2-b]pyridazine.

[0016] Furthermore, the mass ratio of the organic bactericide, the natural bactericide, and the synergist is (8-13):(18-27):(0.8-1.6).

[0017] This invention provides a method for preparing the reduced-dosage, enhanced-efficiency bactericide described in the above technical solution, comprising the following steps:

[0018] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with ethanol solution and then subjected to ultrasonic treatment to obtain a natural bactericide.

[0019] (2) The natural bactericide obtained in step (1), 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2 carboxylic acid and synergist are mixed evenly to obtain the reduced-dosage and enhanced-efficacy bactericide.

[0020] The present invention also provides the application of the reduced-dosage and enhanced-efficiency fungicide described in the above technical solution in the prevention and control of rapeseed black spot disease.

[0021] Furthermore, the reduced-volume, enhanced-efficiency bactericide is diluted to a 900-1200 times dilution before use.

[0022] Compared with the prior art, the present invention has the following advantages and technical effects:

[0023] This invention combines the high efficiency and rapid bactericidal effect of organic fungicides with the advantages of natural fungicides, such as high safety, low resistance, and biodegradability. This achieves excellent control effects while reducing the amount of organic fungicide used. Furthermore, by incorporating synergists, the surface tension of the fungicide on plants is effectively reduced, the utilization rate of active ingredients is improved, and the amount of fungicide used is reduced, resulting in a fungicide with low dosage and good control effect.

[0024] The fungicide provided by this invention, when diluted to 900-1200 times, can still achieve excellent control effect against rapeseed black spot disease, with a control effect of 67.3-80.8%. Detailed Implementation

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to specific embodiments.

[0027] This invention provides a fungicide with reduced dosage and increased efficacy, comprising an organic fungicide, a natural fungicide, and a synergist; the mass ratio of the organic fungicide, the natural fungicide, and the synergist is (8-15):(15-30):(0.5-2), more preferably (8-13):(18-27):(0.8-1.6). This invention combines organic and natural fungicides, integrating the high efficiency and rapid bactericidal effect of organic fungicides with the high safety, low resistance, and biodegradability of natural fungicides. This achieves excellent control effects while reducing the dosage of organic fungicides. Furthermore, the addition of a synergist effectively reduces the surface tension of the fungicide on plants, improves the utilization rate of the active ingredients, and further reduces the dosage, resulting in a fungicide with low dosage and good control effect.

[0028] In a preferred embodiment, the organic fungicide comprises 3,6-disubstituted imidazole[1,2-b]pyridazine derivatives and phenazine α-2-carboxylic acids. The organic fungicide used in this invention is a 3,6-disubstituted imidazole[1,2-b]pyridazine derivative and a phenazine α-2-carboxylic acid, both of which have good inhibitory effects on black spot pathogens and exhibit good persistence and broad-spectrum activity in field applications.

[0029] In a preferred embodiment, the mass ratio of the 3,6-disubstituted imidazole[1,2-b]pyridazine derivative to phenazine α-2-carboxylic acid is (5-10):(1-3), more preferably (6-9):(2-3).

[0030] In a preferred embodiment, the 3,6-disubstituted imidazo[1,2-b]pyridazine derivative is selected from one or more of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, 3,6-diphenylimidazo[1,2-b]pyridazine, 6-chloro-3-(2-bromophenyl)imidazo[1,2-b]pyridazine, and 3-(benzo[b]thiophen-2-yl)-6-chloroimidazo[1,2-b]pyridazine, and more preferably one or more of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, 3,6-diphenylimidazo[1,2-b]pyridazine, and 3-(benzo[b]thiophen-2-yl)-6-chloroimidazo[1,2-b]pyridazine.

[0031] In a preferred embodiment, the natural fungicide includes garlic, bamboo leaves, fennel, and mint. The natural fungicide in this invention uses garlic, bamboo leaves, fennel, and mint. Specifically, the active ingredients in garlic can inhibit the growth and reproduction of pathogens and promote plant growth; the active ingredients in bamboo leaves show good inhibitory effects on various bacteria and fungi; the active ingredients in fennel can inhibit mycelial growth and spore germination by disrupting the cell membrane of pathogenic spores; and the active ingredients in mint can inhibit the growth and reproduction of pathogens. This invention uses natural fungicides as the main raw materials for fungicide production, which has the advantages of being green and environmentally friendly.

[0032] In a preferred embodiment, the mass ratio of garlic, bamboo leaves, fennel and mint is (3-8):(2-5):(1-4):(1-4), and more preferably (4-7):(3-5):(2-4):(2-4).

[0033] In a preferred embodiment, the synergist includes curcumin and matrine. Both curcumin and matrine have antibacterial activity and can inhibit the growth and reproduction of pathogens. At the same time, when matrine is combined with fungicides, it can effectively reduce the surface tension of fungicides on plants, improve the utilization rate of active ingredients, and reduce the amount of fungicide used.

[0034] In a preferred embodiment, the mass ratio of curcumin to matrine is (3-6):(2-4), and more preferably (4-6):(2-3).

[0035] This invention provides a method for preparing the reduced-dosage, enhanced-efficiency bactericide described in the above technical solution, comprising the following steps:

[0036] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with ethanol solution, then subjected to ultrasonic treatment, filtered and evaporated to remove ethanol, to obtain a natural bactericide.

[0037] (2) The natural bactericide obtained in step (1), 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2 carboxylic acid and synergist are mixed evenly to obtain the reduced-dosage and enhanced-efficacy bactericide.

[0038] In a preferred embodiment, in step (1), the volume concentration of the ethanol solution is 60-75%; the ratio of the total mass of garlic, bamboo leaves, fennel and mint to the volume of the ethanol solution is 1:(4-6)(g / mL).

[0039] In a preferred embodiment, in step (1), the ultrasonic treatment power is 200-400W, the temperature is 30-50℃, and the time is 20-50 minutes.

[0040] In a preferred embodiment, in step (2), an emulsifier and water are added during the process of uniformly mixing the natural bactericide, 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2-carboxylic acid, and synergist; the amount of emulsifier is 2-5 wt% of the total mass of the natural bactericide, 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2-carboxylic acid, and synergist; the amount of water is 8-12 wt% of the total mass of the natural bactericide, 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2-carboxylic acid, and synergist; the emulsifier is selected from one or more of Span-60#, Tween-60#, and TX-10.

[0041] The present invention also provides the application of the reduced-dosage and enhanced-efficiency fungicide described in the above technical solution in the prevention and control of rapeseed black spot disease.

[0042] In a preferred embodiment, the reduced-dosage, enhanced-efficacy bactericide is diluted to a 900-1200 times solution before use.

[0043] Unless otherwise specified, all raw materials used in the embodiments of this invention were purchased through commercial channels.

[0044] Example 1

[0045] A fungicide with reduced dosage and increased efficacy is composed of an organic fungicide, a natural fungicide, and a synergist in a mass ratio of 8:25:1.2; wherein the organic fungicide is composed of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine and phenazine α-2-carboxylic acid in a mass ratio of 9:2, the natural fungicide is composed of garlic, bamboo leaves, fennel, and mint in a mass ratio of 3:4:2:2, and the synergist is composed of curcumin and matrine in a mass ratio of 6:4.

[0046] The preparation method of a fungicide with reduced dosage and increased efficacy is as follows:

[0047] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with 68% ethanol solution at a ratio of 1:4 (g / mL). Then, the mixture is ultrasonically treated at 320W and 38℃ for 35 minutes, filtered and evaporated to remove the ethanol, thus obtaining a natural bactericide.

[0048] (2) The natural bactericide obtained in step (1), 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin, matrine, Tween-60# and water are mixed evenly to obtain a bactericide with reduced dosage and enhanced efficacy; wherein, the amount of Tween-60# is 3wt% of the total mass of the natural bactericide, 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine, and the amount of water is 9wt% of the total mass of the natural bactericide, 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine.

[0049] Example 2

[0050] A fungicide with reduced dosage and increased efficacy is composed of an organic fungicide, a natural fungicide, and a synergist in a mass ratio of 13:27:1.6; wherein the organic fungicide is composed of 3-(benzo[b]thiophene-2-yl)-6-chloroimidazole[1,2-b]pyridazine and phenazine α-2-carboxylic acid in a mass ratio of 8:3, the natural fungicide is composed of garlic, bamboo leaves, fennel, and mint in a mass ratio of 6:3:2:3, and the synergist is composed of curcumin and matrine in a mass ratio of 5:3.

[0051] The preparation method of a fungicide with reduced dosage and increased efficacy is as follows:

[0052] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with 72% ethanol solution at a ratio of 1:5 (g / mL). Then, the mixture is ultrasonically treated at 280W and 42℃ for 25 minutes, filtered and evaporated to remove the ethanol, thus obtaining a natural bactericide.

[0053] (2) The natural bactericide obtained in step (1), 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin, matrine, Tween-60# and water are mixed evenly to obtain a bactericide with reduced dosage and enhanced efficacy; wherein, the amount of Tween-60# is 2wt% of the total mass of the natural bactericide, 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine, and the amount of water is 10wt% of the total mass of the natural bactericide, 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine.

[0054] Example 3

[0055] A fungicide with reduced dosage and increased efficacy is composed of an organic fungicide, a natural fungicide, and a synergist in a mass ratio of 11:25:1.4; wherein the organic fungicide is composed of 3,6-diphenylimidazo[1,2-b]pyridazine and phenazine α-2-carboxylic acid in a mass ratio of 8:3, the natural fungicide is composed of garlic, bamboo leaves, fennel, and mint in a mass ratio of 7:4:3:3, and the synergist is composed of curcumin and matrine in a mass ratio of 5:2.

[0056] The preparation method of a fungicide with reduced dosage and increased efficacy is as follows:

[0057] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with 70% ethanol solution at a ratio of 1:6 (g / mL). Then, the mixture is ultrasonically treated at 280W and 38℃ for 42 minutes, filtered and evaporated to remove the ethanol, thus obtaining a natural bactericide.

[0058] (2) The natural bactericide obtained in step (1), 3,6-diphenylimidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin, matrine, TX-10 and water are mixed evenly to obtain a bactericide with reduced dosage and enhanced efficacy; wherein, the amount of TX-10 is 3wt% of the total mass of the natural bactericide, 3,6-diphenylimidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine, and the amount of water is 9wt% of the total mass of the natural bactericide, 3,6-diphenylimidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine.

[0059] Example 4

[0060] A fungicide with reduced dosage and increased efficacy is composed of an organic fungicide, a natural fungicide, and a synergist in a mass ratio of 13:27:1.6; wherein the organic fungicide is composed of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine and phenazine α-2-carboxylic acid in a mass ratio of 7:2, the natural fungicide is composed of garlic, bamboo leaves, fennel, and mint in a mass ratio of 8:4:1:1, and the synergist is composed of curcumin and matrine in a mass ratio of 3:3.

[0061] The preparation method of a fungicide with reduced dosage and increased efficacy is as follows:

[0062] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with 65% ethanol solution at a ratio of 1:4 (g / mL). Then, the mixture is ultrasonically treated at 350W and 48℃ for 45 minutes, filtered and evaporated to remove ethanol, thus obtaining a natural bactericide.

[0063] (2) The natural bactericide obtained in step (1), 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin, matrine, TX-10 and water are mixed evenly to obtain a bactericide with reduced dosage and enhanced efficacy; wherein, the amount of TX-10 is 4 wt% of the total mass of the natural bactericide, 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine, and the amount of water is 10 wt% of the total mass of the natural bactericide, 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine.

[0064] Example 5

[0065] A fungicide with reduced dosage and increased efficacy is composed of an organic fungicide, a natural fungicide, and a synergist in a mass ratio of 15:18:0.8; wherein the organic fungicide is composed of 3-(benzo[b]thiophene-2-yl)-6-chloroimidazole[1,2-b]pyridazine and phenazine α-2-carboxylic acid in a mass ratio of 10:3, the natural fungicide is composed of garlic, bamboo leaves, fennel, and mint in a mass ratio of 4:4:2:1, and the synergist is composed of curcumin and matrine in a mass ratio of 4:3.

[0066] The preparation method of a fungicide with reduced dosage and increased efficacy is as follows:

[0067] (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with 72% ethanol solution at a ratio of 1:5 (g / mL). Then, the mixture is ultrasonically treated at 400W and 45℃ for 35 minutes, filtered and evaporated to remove the ethanol, thus obtaining a natural bactericide.

[0068] (2) The natural bactericide obtained in step (1), 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin, matrine, Tween-60# and water are mixed evenly to obtain a bactericide with reduced dosage and enhanced efficacy; wherein, the amount of Tween-60# is 4 wt% of the total mass of the natural bactericide, 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine, and the amount of water is 11 wt% of the total mass of the natural bactericide, 3-(benzo[b]thiophen-2-yl)-6-chloroimidazole[1,2-b]pyridazine, phenazine α-2-carboxylic acid, curcumin and matrine.

[0069] Comparative Example 1

[0070] A fungicide is composed of an organic fungicide and a natural fungicide in a mass ratio of 11:25, wherein the organic fungicide is composed of 3,6-diphenylimidazole[1,2-b]pyridazine and phenazine α-2 carboxylic acid in a mass ratio of 8:3, and the natural fungicide is composed of garlic, bamboo leaves, fennel and mint in a mass ratio of 7:4:3:3.

[0071] The preparation method of the bactericide is the same as in Example 3.

[0072] Comparative Example 2

[0073] The difference from Example 3 is that the bactericide is composed of organic bactericide, natural bactericide and synergist in a mass ratio of 5:35:1.4, and the rest is the same as in Example 3.

[0074] Comparative Example 3

[0075] The difference from Example 3 is that the natural bactericide is composed of garlic and mint in a mass ratio of 7:3, otherwise it is the same as Example 3.

[0076] Comparative Example 4

[0077] The difference from Example 3 is that the natural bactericide is composed of garlic, bamboo leaves and fennel in a mass ratio of 7:4:3, and the synergist is matrine, and the rest is the same as in Example 3.

[0078] Comparative Example 5

[0079] The difference from Example 3 is that the synergist is curcumin, otherwise it is the same as Example 3.

[0080] The fungicides in Examples 1-5 and Comparative Examples 1-5 were diluted at a ratio of 1:1000, and sprayed evenly on both sides of the rapeseed leaves during the seedling to flowering stage. The application rate was 220 mL per acre, sprayed once every 7-9 days, for a total of 3 applications. A control group was also set up, sprayed with water in the same way and at the same rate.

[0081] Each group had 4 replicates, with 100 leaves investigated per replicate and an area of ​​10 square meters per replicate. The black spot disease index of the rapeseed plants was assessed 14 days after the last spray.

[0082] In the calculation of the disease index of rapeseed black spot disease, the criteria for determining the number of diseased leaves at each level and the corresponding level are based on the severity of the disease. The grading criteria for rapeseed black spot disease in this invention are shown in Table 1.

[0083] Table 1 Grading Standards for Black Spot Disease in Rapeseed

[0084]

[0085] According to the above grading criteria, the formula for calculating the disease index is:

[0086]

[0087] The formula for calculating the prevention and control effect is:

[0088]

[0089] In the above formula, the experimental group refers to the example group or the comparative group.

[0090] Table 2 shows the disease index and prevention and control effects of each group in Examples 1-5 and Comparative Examples 1-5.

[0091] Table 2. Disease index and prevention and control effect results for each group.

[0092] Disease index Prevention and control efficacy / % Example 1 13.3 78.1 Example 2 15.6 74.3 Example 3 11.7 80.8 Example 4 16.0 73.7 Example 5 19.9 67.3 Comparative Example 1 53.2 12.5 Comparative Example 2 46.8 23.0 Comparative Example 3 42.0 30.9 Comparative Example 4 40.9 32.7 Comparative Example 5 38.5 36.7 Control group (water) 60.8 -

[0093] As shown in Table 2, the synergistic and reduced-dosage antibacterial agent provided by this invention has excellent control effects against rapeseed black spot disease, with a control effect of 67.3-80.8%. Furthermore, compared to the control group sprayed with water, the disease index in Example 3 decreased by 49.1. Comparative Examples 1-5, which changed the composition or ratio of the fungicide, showed significantly reduced control effects, indicating that the antibacterial agent provided by this invention has excellent control effects against rapeseed black spot disease.

[0094] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A bactericide with reduced dosage and increased efficacy, characterized in that, The bactericide includes organic bactericides, natural bactericides, and synergists; the mass ratio of the organic bactericide, natural bactericide, and synergist is (8-15):(15-30):(0.5-2); The organic bactericides include 3,6-disubstituted imidazole[1,2-b]pyridazine derivatives and phenazine α-2-carboxylic acid; The natural bactericides include garlic, bamboo leaves, fennel, and mint.

2. The bactericide with reduced dosage and increased efficacy according to claim 1, characterized in that, The mass ratio of the 3,6-disubstituted imidazole[1,2-b]pyridazine derivative to phenazine α-2-carboxylic acid is (5-10):(1-3).

3. The bactericide with reduced dosage and increased efficacy according to claim 1, characterized in that, The mass ratio of garlic, bamboo leaves, fennel and mint is (3-8):(2-5):(1-4):(1-4).

4. The bactericide with reduced dosage and increased efficacy according to claim 1, characterized in that, The synergists include curcumin and matrine.

5. The bactericide with reduced dosage and increased efficacy according to claim 4, characterized in that, The mass ratio of curcumin to matrine is (3-6):(2-4).

6. The bactericide with reduced dosage and enhanced efficacy according to claim 1 or 2, characterized in that, The 3,6-disubstituted imidazo[1,2-b]pyridazine derivatives are selected from one or more of 6-chloro-3-(2,3-dichlorophenyl)imidazo[1,2-b]pyridazine, 3,6-diphenylimidazo[1,2-b]pyridazine, 6-chloro-3-(2-bromophenyl)imidazo[1,2-b]pyridazine, and 3-(benzo[b]thiophen-2-yl)-6-chloroimidazo[1,2-b]pyridazine.

7. The bactericide with reduced dosage and increased efficacy according to claim 1, characterized in that, The mass ratio of the organic bactericide, natural bactericide, and synergist is (8-13):(18-27):(0.8-1.6).

8. The method for preparing the reduced-dosage, enhanced-efficacy bactericide according to any one of claims 1 to 7, characterized in that, Includes the following steps: (1) After crushing and sieving garlic, bamboo leaves, fennel and mint, they are mixed with ethanol solution and then subjected to ultrasonic treatment to obtain a natural bactericide. (2) The natural bactericide obtained in step (1), 3,6-disubstituted imidazole [1,2-b]pyridazine derivative, phenazine α-2 carboxylic acid and synergist are mixed evenly to obtain the reduced-dosage and enhanced-efficacy bactericide.

9. The application of the reduced-dosage, enhanced-efficacy fungicide as described in any one of claims 1 to 7 in the control of rapeseed black spot disease.

10. The application of the reduced-dosage, enhanced-efficacy fungicide according to claim 9 in the control of rapeseed black spot disease, characterized in that, The reduced-dosage, enhanced-efficiency bactericide should be diluted to a 900-1200 times solution before use.

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