Composition with effects of improving skin color and resisting oxidation as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with effects of improving skin color and resisting oxidation as well as a preparation method and application of the composition. The composition with the effects of improving the skin color and resisting oxidation, provided by the invention, is prepared by compounding an ormosia henryi bark extract, a mulberry root bark extract, a raspberry fruit extract, 4-butylresorcinol and ascorbyl tetraisopalmitate; wherein in the preparation method of the ormosia henryi bark extract, the ormosia henryi bark is extracted by adopting a mode of combining eutectic emulsion standing extraction with micro-jet secondary extraction, the stability of active substances in the obtained ormosia henryi bark extract is good, and the extraction efficiency is high; the extraction method is low in production cost and easy to operate, and conforms to the green chemistry principle. The composition provided by the invention has excellent effects of improving skin color and resisting oxidation and is mild and non-irritant, the preparation method is simple and easy to operate, and industrial actual large-scale production and application are facilitated.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition with skin tone improvement and antioxidant effects, its preparation method, and its application. Background Technology

[0002] As we age, various bodily functions begin to decline, the most obvious manifestation being dull skin and signs of aging. Scientific research indicates three main reasons for this: First, excessive exposure to ultraviolet radiation or even ionizing radiation leads to the accumulation of free radicals in the body, which are then eliminated relatively slowly. Second, irregular sleep patterns, excessive stress, and weakened immunity cause poor circulation and insufficient blood and qi. Third, with age, metabolism slows down, resulting in significant collagen loss.

[0003] To address these skin concerns, current skincare products primarily utilize chemical ingredients such as Vitamin C, salicylic acid, and retinol. While these ingredients offer rapid improvement in skin tone and provide antioxidant benefits, they also carry a risk of allergic reactions. Some products use plant extracts as active ingredients, which are natural and gentle, but their effects on skin tone improvement and antioxidant activity are less pronounced. Furthermore, most existing products on the market suffer from drawbacks such as overly simplistic efficacy, limited active ingredients, or low bioavailability of active ingredients, resulting in limited overall improvement.

[0004] In conclusion, developing a composition that can quickly and effectively improve skin tone and provide antioxidant effects while being gentle and non-irritating, in order to better achieve product upgrades, is one of the common goals pursued by the cosmetics industry today. Summary of the Invention

[0005] In view of the above-mentioned defects or improvement needs of the prior art, the present invention provides a composition with skin tone improvement and antioxidant effects, a preparation method thereof and application thereof, solving the problems of poor skin tone improvement, antioxidant and anti-aging effects and strong irritation of existing products.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] In a first aspect, the present invention provides a composition having skin-improving and antioxidant effects, comprising the following components: Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol, and ascorbate tetraisopalmitate; wherein the weight ratio of Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol, and ascorbate tetraisopalmitate is (15-18):(12-15):(7-10):(6-9):(8-10);

[0008] The preparation method of the extract from the bark of the rosewood tree includes the following steps:

[0009] A1. After mixing and homogenizing the hydrogen bond acceptor, hydrogen bond donor and surfactant, add deionized water and stir until homogeneous to obtain a eutectic emulsion.

[0010] A2. After chopping the bark of the rosewood tree, add it to the eutectic emulsion and grind it into a homogenate with liquid nitrogen. After standing extraction, use microfluidic jet for secondary extraction to obtain crude extract.

[0011] A3. The crude extract is centrifuged, filtered, and the supernatant is concentrated to 30% of its original volume to obtain the Dalbergia odorifera bark extract.

[0012] Dalbergia odorifera, also known as rosewood, belongs to the Papilionoideae subfamily of the Fabaceae family and the genus Dalbergia. Its roots, root bark, stems, and leaves can all be used in medicine. The main components of Dalbergia odorifera bark extract are flavonoids, which have various pharmacological activities such as antibacterial, antitumor, and antidepressant effects, and have development and research value.

[0013] Mulberry root bark, also known as mulberry white bark, is a traditional Chinese medicine. The mulberry root bark extract prepared by this application using ethanol ultrasonic-assisted extraction mainly contains isopentenyl flavonoids, benzofurans, polyhydroxy alkaloids and stilbene, and has multiple activities such as antioxidant, anti-inflammatory, hypoglycemic, hypotensive and antiviral effects.

[0014] Raspberry is the fruit of *Rubus palmatus*, a species of raspberry in the Rosaceae family. It has a sweet and sour taste and is warm in nature. The main components of raspberry include flavonoids and their glycosides, terpenes, alkaloids, volatile compounds, coumarins, sterols, phenolic acids, polysaccharides, and aromatic compounds, among which kaempferol-3-O-rutinoside and ellagic acid are typical components. The raspberry fruit extract prepared by the double reflux extraction of ethanol in this application has anti-inflammatory, antioxidant, antibacterial, anti-aging, and whitening effects.

[0015] 4-Butylresorcinol is an ingredient commonly used for skin whitening and treating pigmentation disorders. By inhibiting the activity of tyrosinase to reduce melanin production, 4-Butylresorcinol is widely used in products that improve uneven skin tone, age spots, freckles, and other problems.

[0016] Ascorbate tetraisopalmitate is a fat-soluble derivative of vitamin C with antioxidant, skin-brightening, and collagen-promoting effects. It has attracted widespread attention due to its high stability and strong transdermal absorption.

[0017] Eutectic emulsions are microemulsion systems based on lipid-soluble eutectic solvents (DES) used for the efficient extraction and delivery of various active ingredients from the bark of *Dalbergia odorifera*. By screening specific combinations of hydrogen bond donors and acceptors, DES with good lipid solubility is prepared as a non-polar phase, which then synergistically self-assembles with surfactants and deionized water to form a thermodynamically stable microemulsion. Eutectic emulsions combine the high dispersibility of microemulsion systems with the strong solubility of DES, enabling the simultaneous extraction of both hydrophilic and hydrophobic active ingredients from *Dalbergia odorifera* bark. In-situ microencapsulation is achieved during extraction, significantly improving the stability and bioavailability of active substances. The extraction process is easy to operate and conforms to green chemistry principles.

[0018] Microjets drive the liquid feed through high pressure to generate ultra-high-speed shearing, cavitation, impact and turbulence effects in micron-level channels, thereby achieving efficient cell wall disruption, full release of active ingredients and uniformity of system particle size.

[0019] Preferably, in step A1, the hydrogen bond acceptor is coconut oil and the hydrogen bond donors are babassu seed oil and palm kernel oil.

[0020] Preferably, the molar ratio of the coconut oil, babassu seed oil and palm kernel oil is 1:2-3:1-2; the surfactant is sucrose polystearate.

[0021] Preferably, the weight ratio of the palm bark extract, mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate is 17:14:9:7:9.

[0022] Preferably, the molar ratio of the cocoacid, babassu seed oil and palm kernel oil is 1:2.5:1.5.

[0023] Preferably, in the eutectic emulsion of step A1, the weight percentage of hydrogen bond acceptors and hydrogen bond donors is 8%-17%, the weight percentage of surfactant is 25%-45%, and the balance is deionized water.

[0024] Preferably, in step A2, the ratio of the walnut bark to the eutectic emulsion is 1g:15-25mL.

[0025] Preferably, the static extraction time in step A2 is 20-40 minutes.

[0026] Preferably, in step A2, the pressure of the microjet treatment is 80-120 MPa, the temperature of the microjet treatment is 30-40°C, the number of microjet treatment cycles is 2-4, and the time of each cycle is 60-120 s.

[0027] Preferably, the mulberry root bark extract is an ethanol extract of mulberry root bark, and the raspberry fruit extract is an ethanol extract of raspberry fruit.

[0028] In a second aspect, the present invention provides a method for preparing the composition described in the first aspect, the method comprising the following steps: weighing each component according to the formula amount, mixing and homogenizing to obtain the composition.

[0029] Thirdly, the present invention provides the use of the composition described in the first aspect or the composition prepared by the preparation method described in the second aspect in the preparation of skin care products having skin tone improvement and antioxidant effects.

[0030] Preferably, the skincare product is a serum, gel, lotion, mask, or cream.

[0031] The beneficial effects of this invention are:

[0032] 1. The present invention uses a combination of Dalbergia odorifera bark extract, mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate to obtain a composition that has excellent skin tone improvement and antioxidant effects, and is gentle and non-irritating.

[0033] 2. This invention uses a combination of eutectic emulsion static extraction and microfluidic secondary extraction to extract the bark of the Dalbergia odorifera tree. The resulting Dalbergia odorifera bark extract has good stability of active substances and high extraction efficiency. Furthermore, this extraction method has low production cost, is easy to operate, and conforms to the principles of green chemistry.

[0034] 3. The method for preparing the composition with skin tone improvement and antioxidant effects provided by the present invention is simple and easy to operate, which is conducive to large-scale industrial production and application.

[0035] 4. Applying the composition provided by this invention, which has the effects of improving skin tone and anti-oxidation, to the preparation of skin care products with the effects of improving skin tone and anti-oxidation can meet market demand and has strong market application value. Detailed Implementation

[0036] To further illustrate the technical means and effects of the present invention in achieving the intended purpose, the following detailed description of the specific embodiments, structure, features and effects of the present invention, in conjunction with the composition, is provided below.

[0037] For the following compositions, unless otherwise specified, experimental methods are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise specified, all materials and reagents used are commercially available.

[0038] Some of the raw materials and their sources are as follows:

[0039] The bark of the rosewood tree was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0040] The mulberry root bark powder was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0041] Raspberry fruit powder was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0042] 4-Butylresorcinol was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0043] Ascorbate tetraisopalmitate was purchased from Shanghai Xingye Biotechnology Co., Ltd.

[0044] Mulberry root bark extract

[0045] Its preparation method includes the following steps:

[0046] Weigh the dried mulberry root bark powder, add 70% ethanol solution at a material-to-liquid ratio of 1g:40mL, extract with ultrasonic assistance at 500W for 45min, centrifuge at 8000r / min for 8min, and take the supernatant to obtain the mulberry root bark extract.

[0047] Raspberry fruit extract

[0048] Its preparation method includes the following steps:

[0049] Weigh dried raspberry fruit powder and add 75% ethanol solution at a ratio of 1g:20mL. Reflux for 1 hour and filter under reduced pressure at 0.7MPa. Add 75% ethanol solution to the residue at a ratio of 1g:30mL for a second reflux extraction and filter under reduced pressure at 0.7MPa. Combine the two filtrates and concentrate under reduced pressure at 60℃ until no alcohol odor remains to obtain raspberry fruit extract.

[0050] Extract from the bark of the rosewood tree ①

[0051] Its preparation method includes the following steps:

[0052] A1. After mixing and homogenizing the hydrogen bond acceptor, hydrogen bond donor and surfactant, add deionized water and stir until homogeneous to obtain a eutectic emulsion.

[0053] A2. After chopping the bark of the rosewood tree, add it to the eutectic emulsion and grind it into a homogenate with liquid nitrogen. After standing extraction, use microfluidic jet for secondary extraction to obtain crude extract.

[0054] A3. The crude extract is centrifuged, filtered, and the supernatant is concentrated to 30% of the original volume to obtain the Dalbergia odorifera bark extract.

[0055] In step A1, the hydrogen bond acceptor is coconut oil, and the hydrogen bond donors are babassu seed oil and palm kernel oil; the molar ratio of coconut oil, babassu seed oil and palm kernel oil is 1:2.5:1.5; the surfactant is sucrose polystearate.

[0056] The weight percentage of hydrogen bond acceptors and hydrogen bond donors in the eutectic emulsion of step A1 is 13%, the weight percentage of surfactant is 35%, and the balance is deionized water.

[0057] In step A2, the ratio of the eutectic emulsion to the rosary bark is 1g:20mL.

[0058] The static extraction time in step A2 is 30 minutes;

[0059] In step A2, the pressure of the microfluidic treatment is 100 MPa, the temperature of the microfluidic treatment is 35°C, the number of microfluidic treatment cycles is 3, and the time of each cycle is 90 s.

[0060] Palm bark extract ②

[0061] Its preparation method includes the following steps:

[0062] A1. After mixing and homogenizing the hydrogen bond acceptor, hydrogen bond donor and surfactant, add deionized water and stir until homogeneous to obtain a eutectic emulsion.

[0063] A2. After chopping the bark of the rosewood tree, add it to the eutectic emulsion and grind it into a homogenate with liquid nitrogen. After standing extraction, use microfluidic jet for secondary extraction to obtain crude extract.

[0064] A3. The crude extract is centrifuged, filtered, and the supernatant is concentrated to 30% of the original volume to obtain the Dalbergia odorifera bark extract.

[0065] In step A1, the hydrogen bond acceptor is coconut oil, and the hydrogen bond donors are babassu seed oil and palm kernel oil; the molar ratio of coconut oil, babassu seed oil and palm kernel oil is 1:2:1; the surfactant is sucrose polystearate.

[0066] The weight percentage of hydrogen bond acceptors and hydrogen bond donors in the eutectic emulsion of step A1 is 8%, the weight percentage of surfactant is 25%, and the balance is deionized water.

[0067] In step A2, the ratio of the eutectic emulsion to the rosary bark is 1g:25mL.

[0068] The static extraction time in step A2 is 40 minutes;

[0069] In step A2, the pressure of the microjet treatment is 80 MPa, the temperature of the microjet treatment is 40 °C, the number of microjet treatment cycles is 4, and the time of each cycle is 60 s.

[0070] Palm bark extract ③

[0071] Its preparation method includes the following steps:

[0072] A1. After mixing and homogenizing the hydrogen bond acceptor, hydrogen bond donor and surfactant, add deionized water and stir until homogeneous to obtain a eutectic emulsion.

[0073] A2. After chopping the bark of the rosewood tree, add it to the eutectic emulsion and grind it into a homogenate with liquid nitrogen. After standing extraction, use microfluidic jet for secondary extraction to obtain crude extract.

[0074] A3. The crude extract is centrifuged, filtered, and the supernatant is concentrated to 30% of the original volume to obtain the Dalbergia odorifera bark extract.

[0075] In step A1, the hydrogen bond acceptor is coconut oil, and the hydrogen bond donors are babassu seed oil and palm kernel oil; the molar ratio of coconut oil, babassu seed oil and palm kernel oil is 1:3:2; and the surfactant is sucrose polystearate.

[0076] The weight percentage of hydrogen bond acceptors and hydrogen bond donors in the eutectic emulsion of step A1 is 17%, the weight percentage of surfactant is 45%, and the balance is deionized water.

[0077] In step A2, the ratio of the eutectic emulsion to the rosary bark is 1g:15mL.

[0078] The static extraction time in step A2 is 20 minutes;

[0079] In step A2, the pressure of the microjet treatment is 120 MPa, the temperature of the microjet treatment is 30°C, the number of microjet treatment cycles is 2, and the time of each cycle is 120 s.

[0080] Dalbergia odorifera bark extract ④

[0081] The only difference between Dalbergia cochinchinensis bark extract ④ and Dalbergia cochinchinensis bark extract ① is that the molar ratio of cocoa oil, babassu seed oil and palm kernel oil in the preparation of Dalbergia cochinchinensis bark extract ④ is 1:1.5:1, while all other conditions are the same as those in Dalbergia cochinchinensis bark extract ①.

[0082] Extract from the bark of the palm tree (⑤)

[0083] The only difference between Dalbergia odorifera bark extract ⑤ and Dalbergia odorifera bark extract ① is that in the preparation of Dalbergia odorifera bark extract ⑤, the molar ratio of coconut oil, babassu seed oil and palm kernel oil is 1:4:2, and all other conditions are the same as those for Dalbergia odorifera bark extract ①.

[0084] Palm bark extract ⑥

[0085] The only difference between Dalbergia cochinchinensis bark extract ⑥ and Dalbergia cochinchinensis bark extract ① is that in the preparation of Dalbergia cochinchinensis bark extract ⑥, step A2 does not use microfluidic extraction for secondary extraction and the static extraction time is extended to 60 min, while other conditions are the same as those for Dalbergia cochinchinensis bark extract ①.

[0086] Palm bark extract ⑦

[0087] Its preparation method includes the following steps:

[0088] A1. Take the bark of the rosewood tree, crush it, and pass it through a 40-mesh sieve to obtain rosewood bark powder;

[0089] A2. Weigh 0.4g of cellulase and 0.8g of pectinase respectively, and add them to a 60% ethanol solution so that the concentration of cellulase and pectinase in the ethanol solution is 4mg / mL, thus obtaining an ethanol solution containing mixed enzymes.

[0090] A3. Weigh 5g of Dalbergia odorifera bark powder, add 20mL of ethanol solution containing mixed enzymes and 30mL of 60% ethanol solution (1:10 liquid-to-solid ratio), adjust pH=4, and extract with ultrasonic assistance at 35℃ for 28min to obtain the Dalbergia odorifera bark extract⑦.

[0091] The composition (parts by weight) of the compositions with skin tone improvement and antioxidant effects in Examples 1-3 of this invention is shown in Table 1 below. Each component was weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0092] Table 1. Components and weight parts of the compositions in Examples 1-3

[0093]

[0094]

[0095] Comparative Example 1

[0096] Compared with Example 1, the difference is that the extract of Dalbergia odorifera bark was not added in Comparative Example 1. The missing amount was made up by mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate in a weight ratio of 14:9:7:9. All other conditions and preparation methods were the same as in Example 1.

[0097] Comparative Example 2

[0098] Compared with Example 1, the difference is that mulberry root bark extract was not added in Comparative Example 2. The missing amount was made up by extracts of Dalbergia odorifera bark, raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate in a weight ratio of 17:9:7:9. All other conditions and preparation methods were the same as in Example 1.

[0099] Comparative Example 3

[0100] Compared with Example 1, the difference is that Raspberry fruit extract was not added in Comparative Example 3. The missing amount was made up by extracts of Dalbergia odorifera bark, mulberry root bark, 4-butylresorcinol and ascorbate tetraisopalmitate in a weight ratio of 17:14:7:9. All other conditions and preparation methods were the same as in Example 1.

[0101] Comparative Example 4

[0102] Compared with Example 1, the difference is that 4-butylresorcinol was not added in Comparative Example 4, and the missing amount was made up by extracts of Dalbergia odorifera bark, mulberry root bark, raspberry fruit extract and ascorbate tetraisopalmitate in a weight ratio of 17:14:9:9. All other conditions and preparation methods were the same as in Example 1.

[0103] Comparative Example 5

[0104] Compared with Example 1, the difference is that ascorbic acid tetraisopalmitate was not added in Comparative Example 5, and the missing amount was made up by extracts of Dalbergia odorifera bark, mulberry root bark, raspberry fruit extract and 4-butylresorcinol in a weight ratio of 17:14:9:7. All other conditions and preparation methods were the same as in Example 1.

[0105] Comparative Example 6

[0106] Compared with Example 1, the difference is that in Comparative Example 6, the weight ratio of Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate is 14:16:6:10:7, while other conditions and preparation methods are the same as in Example 1.

[0107] Comparative Example 7

[0108] Compared with Example 1, the difference is that in Comparative Example 7, Dalbergia odorifera bark extract ④ was used instead of Dalbergia odorifera bark extract ①, while other conditions and preparation methods were the same as in Example 1.

[0109] Comparative Example 8

[0110] Compared with Example 1, the difference is that in Comparative Example 8, Dalbergia odorifera bark extract ⑤ was used instead of Dalbergia odorifera bark extract ①, while other conditions and preparation methods were the same as in Example 1.

[0111] Comparative Example 9

[0112] Compared with Example 1, the difference is that in Comparative Example 9, Dalbergia odorifera bark extract ⑥ was used instead of Dalbergia odorifera bark extract ①, while other conditions and preparation methods were the same as in Example 1.

[0113] Comparative Example 10

[0114] Compared with Example 1, the difference is that in Comparative Example 10, Dalbergia odorifera bark extract ⑦ was used instead of Dalbergia odorifera bark extract ①, while other conditions and preparation methods were the same as in Example 1.

[0115] Example 1: Tyrosinase Activity Inhibition Test

[0116] In the process of melanin production, tyrosinase, as the key rate-limiting enzyme, catalyzes the hydroxylation of L-tyrosine to generate dopa, which is then further oxidized to dopaquinone. Dopaquinone then undergoes spontaneous cyclization via a non-enzymatic reaction and gradually polymerizes to form melanin. By detecting the absorbance change corresponding to the dopaquinone formation rate at a wavelength of 475 nm, the degree of inhibition of tyrosinase catalytic activity by the tested compounds was analyzed.

[0117] This test example refers to "T / GDCA006-2021 Test method for inhibition of tyrosinase activity by cosmetic raw materials (in vitro method)" to detect the inhibition rate of tyrosinase activity of the compositions prepared in Examples 1-3 and Comparative Examples 1-10 of this application, thereby evaluating the inhibitory effect of the compositions on tyrosinase activity.

[0118] The specific experimental procedure is as follows:

[0119] The test substances (compositions prepared in Examples 1-3 and Comparative Examples 1-10) were serially diluted with PBS buffer to a concentration of 0.80 mg / mL; four sets of wells were set in a 96-well microplate, with solvent background wells (T... a Add 40 μL of PBS buffer to the solvent reaction well (T). b Add 40 μL of L-tyrosine solution and 40 μL of PBS buffer to the sample background well (T). c Add 40 μL of sample solution to the sample reaction well (T). d Add 40 μL of L-tyrosine solution and 40 μL of sample solution, and make three replicates for each group; mix thoroughly in each well, incubate at 37℃ for 10 min, then add 20 μL of tyrosinase solution to each well in sequence, mix and react at 37℃ for 5 min, and immediately place in an ELISA reader to measure the absorbance at 475 nm.

[0120] Calculate the tyrosinase activity inhibition rate using the following formula:

[0121]

[0122] In the formula: A a A represents the average absorbance of the solvent background pores. b A represents the average absorbance of the solvent reaction wells; c A represents the absorbance of the sample's bottom well. d The absorbance of the sample reaction well;

[0123] The specific results are shown in Table 2.

[0124] Table 2 Tyrosinase inhibition rate data

[0125]

[0126]

[0127] Example 2: Antioxidant Effect Test

[0128] Free radicals possess strong oxidizing properties and can attack biomolecules such as lipids, proteins, and DNA, causing oxidative damage that leads to cell dysfunction and tissue lesions, manifesting as skin aging and wrinkles. Therefore, the DPPH free radical scavenging ability is an important indicator for evaluating anti-aging activity. The antioxidant and anti-aging efficacy of the compositions prepared in Examples 1-3 and Comparative Examples 1-10 was evaluated by measuring their DPPH free radical scavenging rate.

[0129] The specific testing method is as follows:

[0130] The test substances (compositions prepared in Examples 1-3 and Comparative Examples 1-10) were diluted with anhydrous ethanol to a concentration of 5 mg / mL to prepare a test solution; a 0.2 mmol / L DPPH radical solution was prepared with anhydrous ethanol; 2 mL of the test solution was placed in a test tube for each test group, and 2 mL of the prepared DPPH radical solution was added; 2 mL of anhydrous ethanol was placed in a test tube for the control group, and 2 mL of the prepared DPPH radical solution was added; 2 mL of the test solution was placed in a test tube for the blank group, and 2 mL of anhydrous ethanol was added; after mixing all groups, the mixtures were allowed to react at room temperature in the dark for 30 min, and the absorbance was measured at 517 nm. The scavenging rate was calculated using the following formula:

[0131]

[0132] In the formula: A0 is the absorbance value of the blank group; A1 is the absorbance value of the control group; A x The absorbance values ​​are for the test group; each group was measured in triplicate, and the data are presented as the average value.

[0133] The test results are shown in Table 3.

[0134] Table 3. Antioxidant test results

[0135]

[0136] Compared with Example 1, Comparative Examples 1-5 did not contain one of the following: Dalbergia odorifera bark extract, mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol, and ascorbic acid tetraisopalmitate. In Comparative Example 6, the weight ratio of Dalbergia odorifera bark extract, mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol, and ascorbic acid tetraisopalmitate was not within the range specified in this application. The preparation processes of the extracts in Comparative Examples 7-10 were different from those in Example 1. Among them, the eutectic emulsions of Comparative Examples 7-8 used hydrogen bond acceptors and hydrogen bond donors with different molar ratios. Comparative Example 9 did not use microfluidic extraction for secondary extraction. Comparative Example 10 used the traditional method for preparing Dalbergia odorifera bark extract, namely, enzymatic hydrolysis combined with ultrasound-assisted ethanol extraction.

[0137] As shown in Tables 2-3, the data on tyrosinase inhibition rate and DPPH scavenging rate of Examples 1-3 and Comparative Examples 1-5 indicate that Examples 1-3, which use a combination of five components—Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol, and ascorbate tetraisopalmitate—all exhibit high tyrosinase inhibition rate and DPPH scavenging rate. This suggests that the five components—Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol, and ascorbate tetraisopalmitate—have a synergistic effect, thereby enhancing the skin tone improvement and antioxidant and anti-aging effects of the composition.

[0138] Based on the data from Examples 1-3 and Comparative Example 6 in Tables 2-3, it can be seen that when the weight ratio of Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate is (15-18):(12-15):(7-10):(6-9):(8-10), the composition has a better effect on improving skin tone and antioxidant effect. Moreover, when the weight ratio of Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate is 17:14:9:7:9, the effect is the best.

[0139] Based on the data on tyrosinase inhibition rate and DPPH scavenging rate of Example 1 and Comparative Examples 7-10 in Tables 2-3, it can be seen that the preparation process of the Dalbergia odorifera bark extract also significantly affects the extraction efficiency and effect of the extract. The preparation process of this application is the best method explored by the inventors through multiple experiments, which can effectively improve the extraction efficiency and stability of active ingredients, thereby affecting the tyrosinase inhibition rate and DPPH scavenging rate of the composition, that is, affecting the composition's skin tone improvement, antioxidant and anti-aging effects.

[0140] In summary, the preparation method of the Dalbergia odorifera bark extract provided by this invention employs a combination of eutectic emulsion static extraction and microfluidic secondary extraction to extract the Dalbergia odorifera bark. The resulting Dalbergia odorifera bark extract exhibits good stability of active substances and high extraction efficiency. Furthermore, this method is low-cost, easy to operate, and conforms to the principles of green chemistry. The composition provided by this invention, which has skin-improving and antioxidant effects, is composed of Dalbergia odorifera bark extract, mulberry root bark extract, raspberry fruit extract, 4-butylresorcinol, and ascorbic acid tetraisopalmitate. It has excellent skin-improving and antioxidant effects, is mild and non-irritating, meets market demand, and has strong market application value.

[0141] Example 3: Cytotoxicity Test

[0142] Test substance: The compositions prepared in Examples 1-3 that have skin-improving and antioxidant effects;

[0143] The experiment specifically includes the following steps:

[0144] A stable human immortalized epidermal cell line (HaCaT) was used, and the cell concentration was adjusted to 2.0 × 10⁻⁶. 5 Cells / mL, 100 μL per well was seeded into each 96-well cell culture plate; the 96-well cell culture plate was placed in a CO2 incubator and cultured at 37℃±1℃ and CO2 concentration of 5.0%±1% until cell confluence reached ≥90%; the test substance was diluted with PBS to a concentration of 12.5 mg / L and set up a blank control group (using PBS instead of the test substance) and a test substance group, 100 μL per well, with 6 replicates per group, and the data are presented as averages; timing was started from the beginning of adding the test substance (test substance group) and PBS (blank control group), keeping the time interval between each two columns consistent, ensuring that the intervention time for cells in each group was 5 min; after 5 min of intervention, the test substance was aspirated sequentially, and PBS was added to each well to wash the test substance, washing twice; 100 μL of DMEM medium and 10 μL of CCK-8 solution were added to each well, and the plate was incubated for 2 h; the absorbance (OD) value was measured at 450 nm and the cell viability was calculated according to the following formula:

[0145]

[0146] The experimental results are shown in Table 4;

[0147] Table 4 Cell viability data

[0148]

[0149] The cell survival rates in Examples 1-3 were all higher than 95%, indicating that the compositions provided by the present invention, which have the effects of improving skin color and anti-oxidation, are not toxic to cells, have high safety, and are mild and non-irritating.

[0150] Application example: A serum with skin tone improvement and antioxidant effects.

[0151] The compositions of Examples 1-3 were added to the serums to obtain the serums of Application Examples 1-3.

[0152] The serum with skin-improving and antioxidant effects comprises the following components in weight percentages: 3% of the composition with skin-improving and antioxidant effects, 0.7% sodium polyacrylamide dimethyl taurate, 0.1% xanthan gum, 2.5% cetearyl alcohol, 0.2% carbomer, 0.8% trehalose, 2.6% propylene glycol, 1.0% squalane, 2.0% cyclopentasiloxane, 0.4% vitamin C, 0.5% phenoxyethanol, 0.1% fragrance, and the balance being deionized water.

[0153] The preparation method of the serum with skin-improving and antioxidant effects specifically includes the following steps:

[0154] S1. Sodium polyacrylamide dimethyl taurate, xanthan gum, trehalose, propylene glycol, squalane, and 1 / 2 volume of deionized water were mixed and heated to 80°C and homogenized at 1000 rpm to obtain phase A.

[0155] S2. Cetearyl alcohol, carbomer and cyclopentamethoxysiloxane are mixed and heated to 80°C, and homogenized at 1000 rpm to obtain phase B.

[0156] S3. Mix phase A and phase B and homogenize at 1000 rpm to obtain a mixture;

[0157] S4. Cool the mixture to 35°C, then add vitamin C, phenoxyethanol, fragrance and deionized water, homogenize at 1000 rpm, then add the composition with skin tone improvement and antioxidant effects, stir evenly, and discharge to obtain the essence with skin tone improvement and antioxidant effects.

[0158] Blank application example: Compared with application examples 1-3, the difference is that the essence of the blank application example does not contain the composition with skin tone improvement and antioxidant effects, and uses an equal amount of deionized water instead of the composition. The preparation method is the same as that of application examples 1-3.

[0159] Example 4: Human Trial Evaluation of Effectiveness

[0160] This test case evaluates the skin tone improvement and antioxidant and anti-aging effects of the serum in Application Examples 1-3 and the blank application example.

[0161] Twenty-four participants (healthy, with normal skin, no history of cosmetic allergies, aged 35-45 years) were randomly divided into four groups of six. Volunteers applied 1.5g of serum to their faces morning and evening after cleansing and moisturizing, massaging until absorbed. Follow-up visits were conducted on days 0 and 28. On the day of the follow-up visit, volunteers washed their faces with water without applying any products and sat quietly for 20 minutes in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%. The improvement in facial aging indicators was quantified and photographed using instruments, including a Cutometer 2mm MPA 580 to measure skin elasticity (R2), showing a significant difference in improvement before and after use to determine if the sample had an elasticity effect; and a Skin-Colorimeter CL400 and Visia 7 (IPP) to measure skin brightness, showing a significant difference in improvement before and after use to determine if the sample improved skin brightness.

[0162]

[0163] In the formula: X 使用前 Data for each indicator for each group before use (day 0); X 使用后 Data for each indicator after use (day 28) for each group;

[0164] Among them, the larger the brightness value, the more white the color is, and the smaller the brightness value, the more black the color is. The skin plasticity elasticity value R2 is used to represent the extent to which the skin recovers after being stretched in the test. The larger the R2, the younger the skin and the better the elasticity; conversely, the smaller the R2, the older the skin and the worse the elasticity.

[0165] The results are averaged, as shown in Table 5. Compared with the blank application example, the skin elasticity and skin brightness of application examples 1-3 were significantly improved, indicating that the essence prepared by the composition provided by the technical solution of the present invention can significantly improve facial skin elasticity and color, and has excellent skin tone improvement and antioxidant and anti-aging effects.

[0166] Table 5. Human Trial Evaluation Data

[0167]

[0168]

[0169] The above description is merely a preferred composition of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with preferred compositions, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent compositions without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above compositions based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition having skin-improving and antioxidant effects, characterized in that, It includes the following components: Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate; the weight ratio of Dalbergia odorifera bark extract, Morus alba root bark extract, Raspberry fruit extract, 4-butylresorcinol and ascorbate tetraisopalmitate is (15-18):(12-15):(7-10):(6-9):(8-10); The preparation method of the extract from the bark of the rosewood tree includes the following steps: A1. After mixing and homogenizing the hydrogen bond acceptor, hydrogen bond donor and surfactant, add deionized water and stir until homogeneous to obtain a eutectic emulsion. A2. After chopping the bark of the rosewood tree, add it to the eutectic emulsion and grind it into a homogenate with liquid nitrogen. After standing extraction, use microfluidic jet for secondary extraction to obtain crude extract. A3. The crude extract is centrifuged, filtered, and the supernatant is concentrated to 30% of the original volume to obtain the Dalbergia odorifera bark extract. In step A1, the hydrogen bond acceptor is coconut oil, and the hydrogen bond donors are babassu seed oil and palm kernel oil; the molar ratio of coconut oil, babassu seed oil and palm kernel oil is 1:2-3:1-2; and the surfactant is sucrose polystearate.

2. The composition according to claim 1, characterized in that, The weight ratio of the extracts of the Dalbergia odorifera bark, the mulberry root bark, the raspberry fruit extract, 4-butylresorcinol, and ascorbate tetraisopalmitate is 17:14:9:7:

9.

3. The composition according to claim 1, characterized in that, The molar ratio of the cocoacid, babassu seed oil and palm kernel oil is 1:2.5:1.

5.

4. The composition according to claim 1, characterized in that, In step A1, the weight percentage of hydrogen bond acceptors and hydrogen bond donors in the eutectic emulsion is 8%-17%, the weight percentage of surfactant is 25%-45%, and the balance is deionized water.

5. The composition according to claim 1, characterized in that, In step A2, the ratio of rosewood bark to eutectic emulsion is 1g:15-25mL.

6. The composition according to claim 1, characterized in that, The static extraction time in step A2 is 20-40 minutes.

7. The composition according to claim 1, characterized in that, In step A2, the pressure of the microjet treatment is 80-120 MPa, the temperature of the microjet treatment is 30-40℃, the number of microjet treatment cycles is 2-4, and the time of each cycle is 60-120 s.

8. A method for preparing the composition according to any one of claims 1-7, characterized in that, The preparation method includes the following steps: weighing each component according to the formula amount, mixing and homogenizing to obtain the composition.

9. The use of the composition according to any one of claims 1-7 or the composition prepared by the preparation method according to claim 8 in the preparation of skin care products having skin tone improvement and antioxidant effects.

10. The use of the composition according to claim 9, characterized in that, The skincare products mentioned are serums, gels, lotions, masks, or creams.

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