Protein lipid composite supplement with synergistic hangover-alleviating and liver-protecting functions and preparation method thereof

Abstract

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a proteolipid composite supplement with synergistic hangover alleviating and liver protecting functions and a preparation method thereof. The health-care food comprises the following components in percentage by mass: 10-25% of a Pu'er tea extract, 15-30% of glucose syrup, 5-15% of a hovenia acerba extract, 5-15% of a radix puerariae extract, 8-20% of corn peptide, 5-15% of oyster peptide, 3-10% of earthworm peptide, 1-5% of malic acid, 2-8% of multivitamins, 1-5% of an electrolyte compound, 3-10% of prebiotic fibers and 0.5-3% of gamma-aminobutyric acid. The compound vitamins comprise B1, B2, B6, B12, folic acid and vitamin C; the electrolyte compound is a nanoscale electrolyte compound and is an organic chelate containing potassium, magnesium and zinc; the prebiotic fiber is prepared by compounding galactooligosaccharide and resistant dextrin; through compounding design of active peptide components, plant active ingredients and functional factors, the active peptide improves the activity of key enzymes in ethanol metabolism in a targeted manner, and alcohol decomposition is accelerated.

Description

Technical Field

[0001] This invention belongs to the field of pharmaceutical composition technology, specifically relating to a protein-lipid complex supplement with synergistic hangover relief and liver protection functions and its preparation method. Background Technology

[0002] Currently, hangover remedies and liver protection products mainly rely on single active ingredients or simple compound systems. These include plant extract-based products, such as those containing flavonoids like kudzu root and Japanese raisin tree fruit, which work by inhibiting ethanol absorption or increasing ADH enzyme activity; amino acid / vitamin supplements, such as glutathione and B vitamins, which assist the liver in detoxification and metabolism; and electrolyte hydration products, which replenish potassium, magnesium, and other ions lost after drinking to relieve dehydration symptoms. These products mostly use conventional dosage forms such as tablets and oral liquids, and their ingredients are mainly physically mixed.

[0003] However, in the above-mentioned solutions, plant flavonoids only delay the absorption of ethanol and cannot accelerate the metabolism of acetaldehyde; amino acids protect the liver but have a slow rate of alcohol detoxification and lack the ability to regulate the nervous system; active peptides and easily oxidized components are inactivated in the acidic environment of the stomach, resulting in insufficient intestinal absorption. Summary of the Invention

[0004] The purpose of this invention is to provide a protein-lipid complex supplement with synergistic hangover relief and liver protection functions, and its preparation method, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A protein-lipid complex supplement with synergistic hangover relief and liver protection functions, and its preparation method, comprising the following components by weight percentage:

[0007] Pu-erh tea extract 10-25%, glucose syrup 15-30%, Hovenia dulcis extract 5-15%, Pueraria lobata extract 5-15%, corn peptide 8-20%, oyster peptide 5-15%, earthworm peptide 3-10%, malic acid 1-5%, complex vitamins 2-8%, electrolyte complex 1-5%, prebiotic fiber 3-10%, γ-aminobutyric acid 0.5-3%;

[0008] The Pu-erh tea extract is theaflavins, with a purity ≥90%;

[0009] The multivitamin contains B1, B2, B6, B12, folic acid, and vitamin C;

[0010] The electrolyte complex is a nanoscale electrolyte complex, which is an organic chelate containing potassium, magnesium and zinc;

[0011] The prebiotic fiber is a blend of galactooligosaccharides and resistant dextrin.

[0012] Preferably, the particle size range of the nanoscale electrolyte complex is 50-200 nm, and the organic chelate is an amino acid chelate or a citric acid chelate.

[0013] Preferably, the mass ratio of galactooligosaccharides to resistant dextrin in the prebiotic fiber is (1:1) to (1:3).

[0014] Preferably, the rapid-release γ-aminobutyric acid is encapsulated in biphasic microcapsules, with the rapid-release layer coated with sodium alginate and the sustained-release layer coated with ethyl cellulose.

[0015] A method for preparing a protein-lipid complex supplement with synergistic hangover relief and liver protection functions includes the following steps:

[0016] Step a: Mix corn peptide, oyster peptide and earthworm peptide in a certain proportion, and perform secondary directional enzymatic hydrolysis using a compound protease at a specific pH and temperature to obtain a mixture of active peptides with molecular weight concentrated in the range of 500-1500 Da.

[0017] Step b: The active peptide mixture obtained in step a, Pu-erh tea extract, kudzu root extract, malic acid and γ-aminobutyric acid are used together as materials. Fluidized bed coating technology is used to form primary microcapsules with prebiotic fiber as the first layer of encapsulation wall material. Then, a nano-sized electrolyte complex dispersion is used as the second layer of coating liquid for secondary encapsulation to form composite microcapsules with electrolyte gradient release function.

[0018] Step c: The composite microcapsules obtained in step b are mixed and granulated with Hovenia dulcis extract, glucose syrup, and compound vitamins at low temperature (<40℃) under inert gas protection.

[0019] Step d: The granulated material is dried using microwave vacuum combined drying technology, with the moisture content controlled to ≤5%, to obtain the protein-lipid complex supplement.

[0020] Preferably, the complex protease is a flavor protease and a trypsin.

[0021] Compared with the prior art, the beneficial effects of the present invention are:

[0022] (1) Through the compound design of active peptide components, plant active ingredients and functional factors, active peptides target and enhance the activity of key enzymes in ethanol metabolism, and accelerate alcohol decomposition; theabrownins and kudzu flavonoids synergistically remove free radicals generated by ethanol metabolism and inhibit lipid peroxidation of hepatocytes; electrolyte complexes maintain cell ion homeostasis and relieve dehydration and metabolic disorders; sustained-release GABA precisely regulates the excitability of the central nervous system, and simultaneously achieves the triple effects of alcohol detoxification, liver protection and nerve protection.

[0023] (2) The bilayer microcapsule technology of prebiotic fiber encapsulation + nano-electrolyte coating is adopted. The prebiotic layer dissolves rapidly in the stomach to release active peptides and hangover-relieving components, while the nano-electrolyte layer is slowly absorbed in the intestine, improving bioavailability. The γ-aminobutyric acid encapsulated in the biphasic microcapsule achieves a combination of rapid and slow release, simultaneously combating acute intoxication and hangover reactions. Attached Figure Description

[0024] Figure 1 This is a process flow diagram of the present invention. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Example 1:

[0027] Please see Figure 1 As shown, a protein-lipid complex supplement with synergistic hangover relief and liver protection functions contains the following components by weight percentage:

[0028]

[0029]

[0030] 5% multivitamin, including:

[0031] Components Dosage Notes -Vitamin C 2.5％ L-Ascorbic Acid Vitamin B1 0.5％ Thiamine nitrate Vitamin B2 0.3％ Riboflavin Vitamin B6 0.3％ Pyridoxine hydrochloride Vitamin B12 0.02％ Cyanocobalamin folic acid 0.08％ Pterylglutamic acid

[0032] A method for preparing a protein-lipid complex supplement with synergistic hangover relief and liver protection functions includes the following steps:

[0033] Step a: Targeted enzymatic hydrolysis of active peptides

[0034] Corn peptides (15 kg), oyster peptides (10 kg), and earthworm peptides (6 kg) were mixed in a reactor, deionized water was added to adjust the solid content to 25%, the pH was adjusted to 7.0, and a complex protease (flavor protease: trypsin = 1:2, total addition amount 3‰) was added. After enzymatic hydrolysis at 50℃ for 2 hours, the pH was adjusted to 8.0, and enzymatic hydrolysis was continued at 37℃ for 1 hour. The enzyme was inactivated by boiling water, and ultrafiltration (molecular weight cutoff 1500 Da) was performed to obtain an active peptide solution (HPLC analysis showed that the molecular weight was concentrated in the range of 500-1200 Da).

[0035] Step b: Preparation of gradient release microcapsules

[0036] The peptide solution in step a was concentrated to 40% solids and mixed with Pu-erh tea extract (18kg), kudzu root extract (12kg), malic acid (3kg), and γ-aminobutyric acid microcapsules (2kg). The fluidized bed parameters were set as follows: air inlet temperature 45℃ and atomization pressure 0.3MPa.

[0037] First layer of encapsulation: using a 20% aqueous solution of prebiotic fiber (2kg of galactooligosaccharides + 4kg of resistant dextrin) as the wall material, spraying rate 15mL / min, coating weight gain 30%;

[0038] Second layer of encapsulation: The nano-electrolyte complex (3 kg) was dispersed in a 5% sodium alginate solution and spray-coated to increase the weight by 15%.

[0039] Step c: Low-temperature granulation

[0040] The compound microcapsules, 10 kg of Hovenia dulcis extract, 22 kg of glucose syrup, and 5 kg of compound vitamins were fed into a wet granulator. Under nitrogen protection, 30% ethanol was sprayed in to wet the mixture. The stirring speed was 200 rpm and the temperature was controlled at 38℃ to obtain wet granules with a particle size of 0.8-1.2 mm.

[0041] Step 4: Combined drying

[0042] Microwave vacuum drying parameters: First stage: microwave power 3kW (60℃ / 10min); Second stage: vacuum degree -0.08MPa (45℃ / 30min), granulation through a 60-mesh sieve, nitrogen filling and packaging.

[0043] Example 2:

[0044] The difference from Example 1 is that it contains the following components in weight percentage:

[0045]

[0046] 2% multivitamin complex, including:

[0047] Components Dosage Notes Vitamin C 1.0％ Vitamin B1 0.3％ Other B vitamins and folic acid 0.7％ The proportions are the same as in Example 1.

[0048] A method for preparing a protein-lipid complex supplement with synergistic hangover relief and liver protection functions includes the following steps:

[0049] Step a: Targeted enzymatic hydrolysis of active peptides

[0050] Mix corn peptides (20 kg), oyster peptides (8 kg), and earthworm peptides (3 kg), adjust the solid content to 28%, and pH to 7.2; add a complex protease (flavor protease: trypsin = 2:1, total addition amount 2.5‰); enzymatically hydrolyze at 45℃ for 3 hours → adjust pH to 8.5, enzymatically hydrolyze at 40℃ for 0.5 hours → after enzyme inactivation, ultrafilter (molecular weight cutoff 1000 Da), to obtain a peptide solution with molecular weight concentrated in 600-1300 Da.

[0051] Step b: Preparation of gradient release microcapsules

[0052] The peptide solution was concentrated to 35% solids and mixed with Pu-erh tea extract (10kg), kudzu root extract (15kg), malic acid (1kg), and γ-aminobutyric acid microcapsules (0.5kg).

[0053] First layer of encapsulation: 15% aqueous solution of prebiotic fiber (2kg galactooligosaccharide + 6kg resistant dextrin), spray rate 20mL / min, coating weight gain 25%;

[0054] Second layer of encapsulation: Nanoelectrolyte complex (4 kg) was dispersed in 3% sodium alginate solution, and the coating increased the weight by 10%.

[0055] Step c: Low-temperature granulation

[0056] The composite microcapsules were mixed with Hovenia dulcis extract (8 kg), glucose syrup (30 kg), and compound vitamins (2 kg), and then sprayed with 25% ethanol under nitrogen protection to wet the mixture. The mixture was stirred at 180 rpm and heated to 35°C to obtain wet particles with a particle size of 0.5-1.0 mm.

[0057] Step 4: Combined drying

[0058] Microwave vacuum drying: first stage 4kW (65℃ / 8min) → second stage vacuum -0.09MPa (50℃ / 20min), moisture content ≤4.5%, granulated through a 60-mesh sieve.

[0059] Example 3:

[0060] The difference from Example 1 is that it contains the following components in weight percentage:

[0061]

[0062]

[0063] 8% multivitamins, including:

[0064] Components Dosage Notes Vitamin C 4.0％ Other B vitamins and folic acid 4.0％ The proportions are the same as in Example 1.

[0065] A method for preparing a protein-lipid complex supplement with synergistic hangover relief and liver protection functions includes the following steps:

[0066] Step a: Targeted enzymatic hydrolysis of active peptides

[0067] Mix corn peptides (8 kg), oyster peptides (15 kg), and earthworm peptides (10 kg), adjust the solid content to 20%, and pH to 6.8; add a complex protease (flavor protease: trypsin = 1:1, total addition amount 4‰); enzymatically hydrolyze at 55℃ for 1.5 hours → adjust pH to 7.8, enzymatically hydrolyze at 42℃ for 2 hours → after enzyme inactivation, ultrafilter (molecular weight cutoff 2000 Da), to obtain a peptide solution with molecular weight concentrated in 800-1500 Da.

[0068] Step b: Gradient release microcapsule preparation

[0069] The peptide solution was concentrated to 45% solids and mixed with Pu-erh tea extract (25kg), kudzu root extract (5kg), malic acid (5kg), and γ-aminobutyric acid microcapsules (3kg).

[0070] First layer of encapsulation: 25% aqueous solution of prebiotic fiber (galactooligosaccharide 1.5kg + resistant dextrin 1.5kg), spray rate 10mL / min, coating weight gain 35%;

[0071] Second layer of encapsulation: The nano-electrolyte complex (1 kg) was dispersed in a 7% sodium alginate solution, and the coating increased the weight by 20%.

[0072] Low temperature granulation:

[0073] The composite microcapsules were mixed with Hovenia dulcis extract (15 kg), glucose syrup (15 kg), and compound vitamins (8 kg), and then sprayed with 35% ethanol under nitrogen protection to wet the mixture. The mixture was stirred at 220 rpm and heated to 40°C to obtain wet particles with a particle size of 1.0-1.5 mm.

[0074] Step 4: Combined drying

[0075] Microwave vacuum drying: first stage 2.5kW (55℃ / 15min) → second stage vacuum degree -0.07MPa (40℃ / 40min), moisture ≤4.8%, granulation through 60 mesh sieve.

[0076] Preferably, the thickness of the ethyl cellulose coating of the γ-aminobutyric acid sustained-release layer is increased to 50 μm (30 μm in Example 1), and the action time is extended to 6 hours.

[0077] Experimental example:

[0078] Comparative study of synergistic effects of hangover relief and liver protection:

[0079] Test sample

[0080]

[0081] Experimental methods

[0082] Model establishment: An acute alcohol intoxication model was established by gavage administration of 50% ethanol (8g / kg body weight) to SD rats;

[0083] Intervention protocol: The test sample (100 mg / kg body weight) was administered by gavage immediately after intoxication, while the control group received an equal volume of normal saline;

[0084] Testing indicators:

[0085] Effect of alcohol detoxification: blood ethanol concentration (0 / 2 / 4h), time to soberness (behavioral score);

[0086] Liver protection effect: Serum ALT / AST (liver damage marker) and liver tissue SOD (antioxidant enzyme) levels after 6 hours;

[0087] Neuroprotection: GABA content in brain tissue, nerve excitability (delta wave power on electroencephalogram).

[0088] The experimental results are shown in the table below:

[0089] Table 1: Comparison of key indicators for hangover relief and liver protection (mean ± SD)

[0090]

[0091] * indicates a significant difference from the comparative example (p<0.05), # indicates a significant difference from Example 1 (p<0.05);

[0092] As can be seen from the above, the corn peptide in Example 2 provides abundant glutamine, which accelerates the metabolism of acetaldehyde to acetic acid; the 80nm electrolyte chelate promotes cellular ion balance and shortens wakefulness time;

[0093] The three peptides (corn / oyster / earthworm) in Example 1 synergistically enhance the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), thereby increasing the blood alcohol clearance rate;

[0094] Example 3: Sustained-release GABA significantly increased brain delta wave power and inhibited central alcohol excitability; high-dose theabrownins scavenged free radicals.

[0095] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A protein-lipid complex supplement with synergistic hangover relief and liver protection functions, characterized in that, It contains the following components by mass percentage: Pu-erh tea extract 10-25%, glucose syrup 15-30%, Hovenia dulcis extract 5-15%, Pueraria lobata extract 5-15%, corn peptide 8-20%, oyster peptide 5-15%, earthworm peptide 3-10%, malic acid 1-5%, complex vitamins 2-8%, electrolyte complex 1-5%, prebiotic fiber 3-10%, γ-aminobutyric acid 0.5-3%; The Pu-erh tea extract is theaflavins, with a purity ≥90%; The multivitamin contains B1, B2, B6, B12, folic acid, and vitamin C; The electrolyte complex is a nanoscale electrolyte complex, which is an organic chelate containing potassium, magnesium and zinc; The prebiotic fiber is a blend of galactooligosaccharides and resistant dextrin.

2. The protein-lipid complex supplement with synergistic hangover relief and liver protection function according to claim 1, characterized in that: The particle size range of the nanoscale electrolyte complex is 50-200 nm, and the organic chelate is an amino acid chelate or a citric acid chelate.

3. The protein-lipid complex supplement with synergistic hangover relief and liver protection function according to claim 1, characterized in that: The mass ratio of galactooligosaccharides to resistant dextrin in the prebiotic fiber is (1:1) to (1:3).

4. The protein-lipid complex supplement with synergistic hangover relief and liver protection function according to claim 1, characterized in that: The fast-release γ-aminobutyric acid is encapsulated in biphasic microcapsules, with the immediate-release layer coated with sodium alginate and the sustained-release layer coated with ethyl cellulose.

5. A method for preparing a protein-lipid complex supplement with synergistic hangover relief and liver protection functions, characterized in that, Includes the following steps: Step a: Mix corn peptide, oyster peptide and earthworm peptide in a certain proportion, and perform secondary directional enzymatic hydrolysis using a compound protease at a specific pH and temperature to obtain a mixture of active peptides with molecular weight concentrated in the range of 500-1500 Da. Step b: The active peptide mixture obtained in step a, Pu-erh tea extract, kudzu root extract, malic acid and γ-aminobutyric acid are used together as materials. Fluidized bed coating technology is used to form primary microcapsules with prebiotic fiber as the first layer of encapsulation wall material. Then, a nano-sized electrolyte complex dispersion is used as the second layer of coating liquid for secondary encapsulation to form composite microcapsules with electrolyte gradient release function. Step c: The composite microcapsules obtained in step b are mixed and granulated with Hovenia dulcis extract, glucose syrup, and compound vitamins at low temperature (<40℃) under inert gas protection. Step d: The granulated material is dried using microwave vacuum combined drying technology, and the moisture content is controlled to be ≤5% to obtain a protein-lipid complex supplement.

6. The preparation method of the protein-lipid complex supplement with synergistic hangover relief and liver protection function according to claim 5, characterized in that: The complex protease is a flavor protease and a trypsin.

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