Preparation method of hydroxyproline-rich glycoprotein of chlamydomonas reinhardtii

The four-step extraction process solves the problems of complex and energy-intensive traditional processes, enabling the large-scale preparation of high-content Chlamydomonas reinhardtii hydroxyproline glycoprotein. This provides a safe and efficient collagen alternative for application in the food, health products, cosmetics, and pharmaceutical fields.

CN120943911APending Publication Date: 2025-11-14SHANXI TOUYUN BIOTECHNOLOGY CO LTD
View PDF 0 Cites 0 Cited by

Patent Information

Application Number
CN202511156133.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-18
Publication Date
2025-11-14

AI Technical Summary

Technical Problem

Traditional laboratory extraction processes are complex, energy-intensive, and difficult to scale up for the production of Chlamydomonas reinhardtii-rich hydroxyproline glycoproteins. Furthermore, there is a lack of effective plant-based alternatives to collagen.

Method used

A four-step extraction process was adopted, including cell suspension, centrifugation, vortexing, solubilization and ultrafiltration. Using specific solvents and materials, high-content hydroxyproline glycoprotein was obtained by Sepharose CL-4B gel column chromatography and ultrafiltration purification.

Benefits of technology

We obtained hydroxyproline-rich glycoproteins with a hydroxyproline content as high as 188 amino acid residues per 1000 amino acids, which are suitable for large-scale production and can be applied in the food, health products, cosmetics and pharmaceutical fields.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120943911A_ABST
    Figure CN120943911A_ABST
Patent Text Reader

Abstract

The invention discloses a preparation method of chlamydomonas reinhardtii hydroxyproline-rich glycoprotein, which belongs to the technical field of biological medicine, and comprises the following steps: soaking fresh and alive chlamydomonas reinhardtii cells in an alcoholic solution, re-suspending the cells in a water phase, adding grinding beads, oscillating, centrifugally collecting a white precipitation layer, dissolving the white precipitation layer in a solubilizing solvent, filtering, and drying to obtain the chlamydomonas reinhardtii hydroxyproline-rich glycoprotein. And purifying by adopting ultrafiltration or gel column chromatography to obtain the hydroxyproline-rich glycoprotein of the chlamydomonas reinhardtii. The hydroxyproline content in the prepared hydroxyproline-rich glycoprotein of the chlamydomonas reinhardtii can reach 188 amino acid residues per 1000 amino acid residues, and glycosylation modification is mostly single galactoside, single arabinoside or double arabinoside. The invention provides a microalgae-derived collagen analogue which can be applied to the fields of foods, health foods, cosmetics, medicines and the like.
Need to check novelty before this filing date? Find Prior Art