Method for increasing content of small molecular polysaccharide in polygonatum sibiricum

Abstract

The invention discloses a method for increasing the content of small molecular polysaccharides in rhizoma polygonati, and belongs to the technical field of plant polysaccharide extraction. The method comprises the following steps: blanching fresh rhizoma polygonati tubers, slicing, pre-freezing, freeze-drying and irradiating at the irradiation dose of 5-10 kGy, adding cellulase into hot water of 55-65 DEG C after irradiation, extracting, concentrating the obtained extracting solution, carrying out alcohol precipitation, and separating and precipitating to obtain the small molecular polysaccharide. The polygonatum sibiricum micromolecular polysaccharide product obtained by the method is faint yellow, the molecular weight is lower than 8000Da, and the polysaccharide accounts for more than 80%.

Description

Technical Field

[0001] This invention belongs to the field of plant polysaccharide extraction technology. Specifically, it relates to a processing and modification technology of Polygonatum sibiricum, and more particularly to a method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum through processing and modification. Background Technology

[0002] Polygonatum, a plant of the lily family, is widely distributed in tropical regions outside of southern China and is abundant in resources. Studies have found that Polygonatum contains various beneficial chemical components such as polysaccharides, total phenols, flavonoids, saponins, lignans, and amino acids. Traditional Chinese medicine believes that Polygonatum is sweet and neutral in nature, tonifying qi and kidneys, resisting cold and heat, and strengthening muscles and bones. It can be taken as a long-term tonic without the potential side effects of strong tonics or warming agents. Modern pharmacological research has also proven that Polygonatum has anti-free radical, anti-aging, immune-boosting, memory-improving, blood sugar-lowering, and blood lipid-lowering effects, making it widely used in health foods and possessing broad market prospects.

[0003] As a medicinal and edible herb, Polygonatum sibiricum generally requires processing to reduce toxicity and improve taste. There are numerous processing methods for Polygonatum sibiricum, including single steaming, wine processing, nine-steaming and nine-processing, double steaming, and fermentation, with the "nine-steaming and nine-processing" method being the most representative, typically taking more than 20 days. Existing literature indicates that small-molecule polysaccharides (less than 8000 da) in Polygonatum sibiricum are easily absorbed and have high biological activity, playing an important role in the development of functional products based on Polygonatum sibiricum. Although traditional processing techniques can increase the content of small-molecule polysaccharides in Polygonatum sibiricum, these techniques suffer from drawbacks such as long processing time and high energy consumption, impacting industrial efficiency. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a method that can increase the content of small molecule polysaccharides in Polygonatum odoratum and simplify the process, so as to solve the technical problems of numerous process steps and high energy consumption in the existing process.

[0005] To achieve the above-mentioned technical objectives, the inventors conducted extensive experiments and research and continuously improved the process. Finally, they adopted a method combining freeze-drying technology and irradiation technology, thereby solving the processing problem of Polygonatum odoratum.

[0006] Specifically, the objective of this invention is achieved as follows: a method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum, the method comprising the following steps:

[0007] (1) Place fresh Solomon's Seal tubers in water above 95℃ for 5-10 minutes, remove and dry the surface moisture, slice them to a thickness of 3-6 mm, pre-freeze at -28--32℃ for 12-24 hours, the freezing equipment for pre-freezing can be a refrigerator or freezer, and then perform vacuum freeze-drying until the moisture content is below 5%.

[0008] (2) Irradiate the freeze-dried Polygonatum tablets with an irradiation dose of 5-10 kGy. After irradiation, add cellulase to hot water at 55-65℃ and extract for 2-4 h. The resulting extract is concentrated to obtain a concentrated solution, then ethanol is added for precipitation. The precipitate is separated and freeze-dried to obtain small molecule polysaccharides.

[0009] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the fresh Polygonatum is selected from at least one of the following: Polygonatum sibiricum, Polygonatum multiflorum, and Polygonatum yunnanense, which have been planted for more than 4 years.

[0010] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the conditions for vacuum freeze-drying are: cold trap temperature less than -70°C, vacuum degree less than 5 Pa, and freeze-drying time 72–96 h.

[0011] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the irradiation source is a Co60 isotope.

[0012] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the amount of cellulase added is 4-6 U / g Polygonatum.

[0013] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the material-to-liquid ratio of the extraction is 1:(15-30), the extraction is performed 2-3 times, and the resulting extracts are filtered and combined.

[0014] More preferably, in the method for increasing the content of small molecule polysaccharides in Polygonatum as described above, the extract is concentrated to a mass ratio of (3-4):1 between the concentrate and the freeze-dried Polygonatum tablets.

[0015] More preferably, the method for increasing the content of small molecule polysaccharides in Polygonatum as described above involves adding 3-4 times the volume of 95% ethanol for precipitation, filtering, and then freeze-drying the precipitate to obtain small molecule polysaccharide powder.

[0016] Compared with existing technologies, the extraction method of small molecule polysaccharides from Polygonatum provided by this invention has the following advantages and advancements:

[0017] (1) The small molecule polysaccharide product of Polygonatum obtained by the method of the present invention is light yellow, with a molecular weight of less than 8000 Da and a polysaccharide content of more than 80%;

[0018] (2) The small molecule polysaccharide product of Polygonatum obtained by the method of the present invention has good water solubility;

[0019] (3) The small molecule polysaccharide product of Polygonatum obtained by the method of the present invention has obvious antioxidant effect.

[0020] (4) The polysaccharide product of Polygonatum odoratum obtained by the method of the present invention has a polysaccharide content that is about 56% higher than that obtained by nine steaming and nine processing. Attached Figure Description

[0021] Figure 1 Standard curve for determining the molecular weight of Polygonatum polysaccharides.

[0022] Figure 2 : Molecular weight distribution of polysaccharides from raw Polygonatum sibiricum; The peak area ratio in the figure represents the ratio of polysaccharide content of different molecular weights in the polysaccharide (the same below); The peak elution time of polysaccharides in raw Polygonatum sibiricum is between 9 and 13 min, and the molecular weight range is 20,000-80,000 Da compared with the standard curve.

[0023] Figure 3 Example 1: Molecular weight distribution of small molecule polysaccharide product extracted from Polygonatum sibiricum.

[0024] Figure 4 Molecular weight distribution of small molecule polysaccharide products extracted from Polygonatum sibiricum in Comparative Examples 1-7.

[0025] Figure 5 Example 1-5: Scavenging rate of DPPH free radicals by small molecule polysaccharides extracted from Polygonatum sibiricum. Detailed Implementation

[0026] The present invention is further illustrated below through embodiments. It should be understood that the preparation methods of the embodiments of the present invention are merely for illustrating the technical solutions and effects of the present invention, and are not intended to limit the scope of protection of the present invention. Simple improvements to the preparation methods of the present invention under the premise of the overall concept are all within the scope of protection claimed by the present invention.

[0027] Example 1:

[0028] 1) Processing of fresh Polygonatum raw materials: Select Polygonatum tubers that are 4 years old or older and free from mold and insect infestation, wash off the surface soil, and set aside;

[0029] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 8 minutes, then cool with cold water for later use.

[0030] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 5mm;

[0031] 4) Pre-cooling: Pre-freeze at -30℃ for 18 hours;

[0032] 5) Freeze-drying: Cold trap temperature less than -70℃, vacuum degree less than 5Pa, freeze-drying time 80h;

[0033] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co60 isotope radiation at a dose of 5 Gy.

[0034] 7) Extraction: The extraction parameters are 60℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0035] 8) Concentration: Vacuum concentration until the mass ratio of the concentrate to the freeze-dried Polygonatum tablets is 3:1;

[0036] 9) Alcohol precipitation and drying: Add 3 times the volume of 95% edible ethanol to the concentrate, precipitate, filter, freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0037] Example 2: Same as Example 1, except that...

[0038] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 10 minutes, then cool with cold water for later use.

[0039] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 8 Gy.

[0040] 7) Extraction: The extraction parameters are 55℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0041] 8) Concentration: Vacuum concentration, with the mass ratio of concentrated liquid to freeze-dried Polygonatum tablets being 4:1;

[0042] Example 3: Same as Example 1, except that...

[0043] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 5 minutes, then cool with cold water for later use.

[0044] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 3mm;

[0045] 4) Pre-cooling: Pre-freeze at -30℃ for 18 hours;

[0046] 5) Freeze-drying: Cold trap temperature less than -70℃, vacuum degree less than 5Pa, freeze-drying time 72h;

[0047] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 10 Gy.

[0048] 7) Extraction: The extraction parameters are 55℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0049] 8) Concentration: Vacuum concentration, with the mass ratio of concentrated liquid to freeze-dried Polygonatum tablets being 4:1;

[0050] 9) Alcohol precipitation and drying: Add 4 times the volume of 95% ethanol, precipitate, filter, and freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0051] Example 4: Same as Example 1, except that...

[0052] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 10 minutes, then cool with cold water for later use.

[0053] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 7mm;

[0054] 4) Pre-cooling: Pre-freeze at -30℃ for 18 hours;

[0055] 5) Freeze-drying: Cold trap temperature less than -70℃, vacuum degree less than 5Pa, freeze-drying time 96h;

[0056] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 7 Gy.

[0057] 7) Extraction: The extraction parameters are 65℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0058] 8) Concentration: Vacuum concentration, with the mass ratio of concentrated liquid to freeze-dried Polygonatum tablets being 4:1;

[0059] 9) Alcohol precipitation and drying: The mass ratio of concentrated liquid to material is 4:1. Add 4 times the volume of 95% ethanol to precipitate. After filtration, freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0060] Example 5: Same as Example 1, except that...

[0061] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 8 minutes, then cool with cold water for later use.

[0062] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 5mm;

[0063] 4) Pre-cooling: Pre-freeze at -30℃ for 18 hours;

[0064] 5) Freeze-drying: Cold trap temperature less than -70℃, vacuum degree less than 5Pa, freeze-drying time 80h;

[0065] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 5 Gy.

[0066] 7) Extraction: The extraction parameters are 60℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0067] 8) Concentration: Vacuum concentration, with a mass ratio of concentrate to freeze-dried Polygonatum tablets of 3.5:1;

[0068] 9) Alcohol precipitation and drying: The mass ratio of concentrated liquid to material is 3.5:1. Add 4 times the volume of 95% ethanol to precipitate. After filtration, freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0069] like Figure 2 As shown in the figure, the peak area ratio represents the ratio of polysaccharide content at different molecular weights; the elution time of polysaccharides in raw Polygonatum sibiricum is between 9 and 13 minutes, and the molecular weight range is 20,000-80,000 Da according to the standard curve. Figure 3 As shown, the small molecule polysaccharide powder extracted by the method of the present invention has a peak elution time of 18-23 min, indicating that the molecular weight is less than 8000 and the peak area is relatively uniform.

[0070] Examples of failed experiments experienced by the inventors during the experiment are as follows:

[0071] Comparative Example 1: Same as Example 1, except that:

[0072] 1) Processing of fresh Polygonatum raw materials: Select Polygonatum tubers that are 4 years old or older and free from mold and insect infestation, wash off the surface soil, and set aside;

[0073] 2) Blanching: Place the fresh Solomon's Seal obtained in step 1) into a heated water tank or steamer. Keep the water temperature at a gentle boil. After adding the Solomon's Seal, start timing when the water boils again. Blanch for 8 minutes, then cool with cold water for later use.

[0074] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 5mm;

[0075] 4) Drying: Dry at 60℃ until the moisture content is about 15%;

[0076] 5) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 5 Gy.

[0077] 6) Extraction: The extraction parameters are 60℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0078] 8) Concentration: Vacuum concentration, the mass ratio of concentrate to material is (3-4):1;

[0079] 9) Alcohol precipitation and drying: The mass ratio of concentrated solution to dried Polygonatum sibiricum tablets is (3-4):1. Add 3-4 times the volume of 95% ethanol, precipitate, filter, and freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0080] Reason for failure: The product obtained by using heat drying followed by irradiation had a low proportion of small molecular weight polysaccharides.

[0081] Comparative Example 2: Same as Example 1, except that:

[0082] 1) Processing of fresh Polygonatum raw materials: Select Polygonatum tubers that are 4 years old or older and free from mold and insect infestation, wash off the surface soil, and set aside;

[0083] 2) Slicing: Slice the Polygonatum obtained in step 1 into slices with a thickness of 5mm;

[0084] 4) Pre-cooling: Pre-freeze at -30℃ for 18 hours;

[0085] 5) Freeze-drying: Cold trap temperature less than -70℃, vacuum degree less than 5Pa, freeze-drying time 120h;

[0086] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co-60 isotope radiation at a dose of 5 Gy.

[0087] 7) Extraction: The extraction parameters are 55-65℃, material-to-liquid ratio 1:20, time 3h, cellulase addition 5U / g Polygonatum, extraction twice, and the extracts are combined after filtration;

[0088] 8) Concentration: Vacuum concentration, with a concentrate to material mass ratio of 3:1;

[0089] 9) Alcohol precipitation and drying: The mass ratio of the concentrated solution to the freeze-dried Polygonatum tablets is 3:1. Add 3-4 times the volume of 95% ethanol to precipitate. After filtration, freeze-dry the precipitate to obtain small molecule polysaccharide powder.

[0090] Reason for failure: Direct freeze-drying without blanching resulted in a longer freeze-drying time and a lower polysaccharide extraction rate than in Examples 1-5.

[0091] Comparative Example 3: Same as Example 1, except that:

[0092] 3) Slicing: Slice the Polygonatum obtained in step 2 into slices with a thickness of 10mm;

[0093] Reason for failure: The slices were too thick, which greatly extended the freeze-drying time.

[0094] Comparative Example 4: Same as Example 1, except that:

[0095] 6) Extraction: The extraction parameters are 60℃, material-to-liquid ratio 1:20, time 3h, extraction twice, and the extracts are combined after filtration;

[0096] Reason for failure: Lack of enzymatic hydrolysis resulted in low polysaccharide extraction efficiency.

[0097] Comparative Example 5: Same as Example 1, except that:

[0098] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co60 isotope radiation at a dose of 4 Gy.

[0099] Reason for failure: Insufficient irradiation dose, resulting in insignificant polysaccharide degradation.

[0100] Comparative Example 6: Same as Example 1, except that:

[0101] 6) Irradiation: The Polygonatum sibiricum raw material obtained in step 3) is irradiated with Co60 isotope radiation at a dose of 12 Gy.

[0102] Reason for failure: The irradiation dose was too high, resulting in excessive degradation of polysaccharides and a decrease in yield.

[0103] Comparative Example 7: Same as Example 1, except that:

[0104] 7) Extraction: Without irradiation, the freeze-dried sample is directly extracted with parameters of 55-65℃, material-to-liquid ratio of 1:20, time of 3h, cellulase addition of 5U / g Polygonatum, and extraction is performed twice. The extracts are then filtered and combined.

[0105] Reason for failure: Direct extraction after freeze-drying resulted in low polysaccharide extraction rate and low yield of small molecule polysaccharides.

[0106] The reason for the above failed test cases is that the freeze-drying-irradiation-enzymatic hydrolysis and other treatment steps were not combined, or the treatment time or dosage was insufficient. As can be seen from the above examples and comparative examples, the present invention solves the problem of preparing functional small molecule polysaccharides of Polygonatum odoratum and solves the industry difficulty.

[0107] 1. Molecular weight determination

[0108] HPGPC was used to determine the molecular weight distribution of polysaccharides. Chromatographic conditions: Elite 3100 high-performance liquid chromatograph with evaporator flash detector; TSKgel G4000PWXL analytical column; detector evaporation temperature 40℃, gas flow rate 2.5 L / min; column temperature 30℃; room temperature 25℃; sample concentration 1 mg / mL; flow rate 0.5 mL / min; mobile phase ultrapure water; injection volume 20 μL. Standard curve plotting: Dextran reference standards with different fractions (3000, 5000, 8000, 10000, 20000, 40000, 80000) were prepared into 1 mg / mL reference solutions with distilled water, filtered through a 0.45 μm microporous membrane, and injected under the above chromatographic conditions. The retention times of each reference standard were recorded. After analysis, the logarithm of the weight fraction (MW) of each standard sugar was plotted as the ordinate (lqMW), and the retention time as the abscissa (tR). A standard curve was plotted, and the results are shown in Table 1.

[0109] Table 1 Standard Curve

[0110] Molecular weight Mw lgMw T / min 3000 3.4771 20.83 5000 3.699 19.56 8000 3.9031 18.08 10000 4.0 17.12 20000 4.301 15.03 40000 4.6021 12.16 80000 4.9031 9.52

[0111] 2. Chromatograms and molecular weights of polysaccharides obtained from different embodiments

[0112] Example Percentage of low molecular weight polysaccharides (%) Polysaccharide extraction rate (%) Example 1 85 18 Example 2 91 21 Example 3 87 19 Example 4 90 20 Example 5 86 21 Comparative Example 1 50 15 Comparative Example 2 80 17 Comparative Example 3 85 17 Comparative Example 4 87 13 Comparative Example 5 54 16 Comparative Example 6 92 14 Comparative Example 7 30 14

[0113] Products without irradiation treatment (Comparative Example 7) or with insufficient irradiation dose (Comparative Example 5) have a low proportion of low molecular weight Polygonatum polysaccharides; excessive irradiation dose results in excessively small molecular weights, thus reducing the proportion of polysaccharides that can be precipitated by alcohol. Products without freeze-drying combined with irradiation treatment (Comparative Example 1) show poor preparation results of low molecular weight polysaccharides.

[0114] 3. Antioxidant properties of polysaccharides obtained from different embodiments

[0115] (1) Weigh the polysaccharides obtained from each example, and use the polysaccharides obtained from the untreated polysaccharide sample as a control to prepare a 1 mg / ml solution for testing.

[0116] (2) Preparation of DPPH solution. Using anhydrous ethanol as solvent, weigh 0.0100 g of 2,2-biphenyl-1-picrylhydrazine into a small beaker using an electronic analytical balance. After complete dissolution, transfer to a 50 mL volumetric flask, dilute to volume, shake well, and store in the dark.

[0117] (3) DPPH free radical scavenging test. Add 1.00 mL of Polygonatum polysaccharide solution to each of the five test tubes and label them. Then add 2.00 mL of the prepared DPPH solution to each tube. If the volume is less than 5 mL, add deionized water to the 5 mL mark. Incubate in the dark for 25 min, and measure the absorbance of each treatment using a microplate reader at a wavelength of 517 nm. Calculate the DPPH scavenging rate according to the following formula:

[0118]

[0119] like Figure 5 As shown, the polysaccharide obtained from the Polygonatum sample that was not treated by the method of the present invention had a DPPH free radical scavenging rate of less than 30%, while the Polygonatum polysaccharide prepared in Examples 1-5 of the present invention had a DPPH free radical scavenging rate of more than 70%.

Claims

1. A method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum, characterized in that, The method includes the following steps: (1) Place fresh Solomon's Seal tubers in water above 95℃ for 5-10 minutes, remove and dry the surface moisture, slice them to a thickness of 3-6 mm, pre-freeze at -28--32℃ for 12-24 hours, and then freeze-dry them under vacuum until the moisture content is below 5%. (2) Irradiate the freeze-dried Polygonatum tablets with an irradiation dose of 5-10 kGy. After irradiation, add cellulase to hot water at 55-65℃ and extract for 2-4 h. The resulting extract is concentrated to obtain a concentrated solution, then ethanol is added for precipitation. The precipitate is separated and freeze-dried to obtain small molecule polysaccharides.

2. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The selected fresh yellow is made from at least one of the following: Polygonatum sibiricum, Polygonatum multiflorum, or Polygonatum yunnanense, which have been cultivated for more than 4 years.

3. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The conditions for vacuum freeze-drying are: cold trap temperature less than -70℃, vacuum degree less than 5 Pa, and freeze-drying time 72-96 h.

4. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The radiation source for the irradiation was a Co60 isotope.

5. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The amount of cellulase added is 4-6 U / g Polygonatum.

6. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The extraction process involves a material-to-liquid ratio of 1:(15-30), with extractions performed 2-3 times. The resulting extracts are then filtered and combined.

7. The method for increasing the content of small molecule polysaccharides in Polygonatum sibiricum according to claim 1, characterized in that, The extract is concentrated until the mass ratio of the concentrate to the freeze-dried Polygonatum tablets is (3-4):1.

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