Method for artificially feeding cordyceps sinensis host hepialus armoricanus larvae

Abstract

The invention relates to the technical field of artificial cultivation of medicinal insects, in particular to a method for artificially feeding cordyceps sinensis host hepialus armoricanus larvae. The invention provides a method for artificially feeding cordyceps sinensis host hepialus armoricanus larvae. The method comprises the following steps: sequentially constructing a three-layer structure from bottom to top in an independent small chamber, wherein the three-layer structure comprises an underground sheltering and moisturizing layer, an artificial feed slow-release layer and an earth surface humus activity layer; feeding a single hepialus armoricanus larva in the small chamber; environment control: the temperature is 10-18 DEG C, and the humidity is 75-85%; and feeding management: dynamically feeding artificial feed for slow release and cleaning excrement. According to the method, the survival rate and health degree of the hepialus armoricanus larvae can be remarkably improved, the growth cycle is shortened, and large-scale management is facilitated.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of artificial cultivation of medicinal insects, in particular to a method for artificially feeding host bat moth larvae of Cordyceps sinensis. BACKGROUND

[0002] Cordyceps sinensis is a famous traditional Chinese medicinal material, which is formed by Cordyceps sinensis invading bat moth larvae and growing in the body of the larvae. Its unique medicinal value leads to overexploitation of the market, resulting in wild resources being close to exhaustion and the ecological environment being severely damaged. Therefore, realizing artificial large-scale cultivation of Cordyceps sinensis is a sustainable way to solve the resource shortage and protect the ecology.

[0003] The core difficulty of artificial cultivation of Cordyceps sinensis lies in large-scale and high-survival-rate artificial feeding of its specific host, bat moth larvae. The existing technology often faces the following problems in feeding bat moth larvae: low survival rate of larvae: improper control of soil microenvironment (temperature, humidity, microbial community, physicochemical properties), resulting in a large number of deaths of larvae; long growth cycle: unbalanced or poor palatability of feed, delaying development; frequent diseases: under high-density feeding conditions, bacterial and fungal diseases are prone to outbreak, and the control means are limited and easy to cause drug residues; extensive management: feed is easily contaminated, frequently replaced, environmental regulation relies on experience, and it is difficult to standardize and scale.

[0004] Some existing technologies attempt to improve the survival rate by adding probiotics, but there are still problems such as insufficient environmental control precision and serious feed contamination. Therefore, there is an urgent need for a new feeding system and method that can simulate natural habitat, achieve stable environment, clean feed and efficient management. SUMMARY

[0005] The purpose of the present application is to provide a method for artificially feeding bat moth larvae, which can significantly improve the survival rate and health of bat moth larvae, shorten the growth cycle, and facilitate large-scale management.

[0006] In order to achieve the above-mentioned purpose of the application, the present application provides the following technical solutions:

[0007] The present application provides a method for artificially feeding host bat moth larvae of Cordyceps sinensis, comprising the following steps:

[0008] S1. Constructing a three-layer structure from bottom to top in a separate chamber: underground shelter and moisture layer, artificial feed slow-release layer, and surface humus activity layer;

[0009] S2. Feeding single bat moth larvae in the chamber;

[0010] S3. Environmental control: temperature 10-18℃, humidity 75-85%;

[0011] S4. Feeding management: dynamic feeding of artificial feed slow-release and cleaning of feces.

[0012] Preferably, the underground shelter moisture layer is made of perlite, peat and super absorbent resin mixed in a weight ratio of 40-50:40-50:0.5-1.

[0013] Preferably, the laying thickness of the underground shelter moisture layer is 2.5-3.5 cm; the humidity of the underground shelter moisture layer is 50-60%.

[0014] Preferably, the artificial feed slow-release layer comprises artificial feed slow-release blocks.

[0015] Preferably, the artificial feed slow-release block is obtained by mixing artificial feed and sodium alginate in a mass-volume ratio of 1g:0.8-1.2ml and then cross-linking and solidifying.

[0016] Preferably, the initial concentration of sodium alginate is 3-7wt%.

[0017] Preferably, the artificial feed comprises the following components in parts by weight: Rubia cordifolia powder 15-25 parts, Astragalus root stem powder 10-20 parts, carrot powder 5-15 parts, wheat bran 5-15 parts, silkworm chrysalis powder 5-10 parts, yeast powder 3-8 parts, sucrose 2-5 parts, curcumin 0.1-0.5 parts, compound vitamins 0.5-1.5 parts, mineral salts 0.5-1.5 parts, nipagin ethyl 0.05-0.15 parts, and water 35-45 parts.

[0018] Preferably, the surface humus activity layer is made of coconut husk, perlite, rotten oak leaf powder and earthworm manure mixed in a weight ratio of 50-60:20-30:15-20:5-10, and an observation hole is reserved.

[0019] Preferably, the laying thickness of the surface humus activity layer is 2-3 cm, and the humidity of the surface humus activity layer is 30-40%.

[0020] Preferably, the dynamic feeding method of the artificial feed slow-release block is as follows: initially laying 2.0-3.0g of artificial feed slow-release block; during the feeding process, the consumption is checked regularly, and when the consumption rate reaches 40%-50%, 0.5-1.5g of slow-release block is fed through the observation hole.

[0021] The beneficial effects of the present application are as follows:

[0022] The application provides a method for artificially breeding host bat moth larvae of Ophiocordyceps sinensis, which sets up three layers (shelter layer, feed layer and activity layer), each of which plays its own role and cooperates with each other, so that the natural ecological niche of the larvae is simulated to the maximum, and core problems such as environmental fluctuation, feed pollution and cross infection of diseases are solved, and the survival rate is expected to be increased to more than 85%.

[0023] The application adopts natural favorite plants (Rubia cordifolia and Astragalus) and functional additives (curcumin), so that the nutrition is more comprehensive; the solid slow-release block feeding mode is not easy to be mildewed, reduces waste and reduces the replacement frequency, so that the larvae gnawing behavior is closer to nature; and through the matrix compatibility (earthworm manure probiotics) and feed additives (curcumin), a multi-dimensional green disease prevention and control system is constructed, and the health of the larvae is ensured.

[0024] The application also perfectly solves the contradiction between'single breeding' and 'high-density breeding' through the design of the single-chambered conjoined culture plate, so that the breeding efficiency per unit area is greatly improved while the individual welfare is ensured. DETAILED DESCRIPTION

[0025] The application provides a method for artificially breeding host bat moth larvae of Ophiocordyceps sinensis, which comprises the following steps:

[0026] S1. Constructing three layers from bottom to top in the independent small chamber: underground shelter and moisture retention layer, artificial feed slow-release layer and surface humus activity layer;

[0027] S2. Breeding the bat moth larvae in the small chamber one by one; the breeding mode is single culture by using the conjoined culture plate, and the size of each small chamber is 5-6*5-6*8-10 cm, preferably 5.5*5.5*9 cm; which effectively prevents the adjacent larvae from interfering and biting each other, and realizes the unification of single individual breeding in physical isolation and high-density breeding in space utilization;

[0028] S3. Environmental control: the temperature is 10-18 DEG C, and the humidity is 75-85 %;

[0029] S4. Breeding management: dynamically feeding the artificial feed slow-release and cleaning the feces.

[0030] In the application, the underground shelter and moisture retention layer is made by mixing perlite, peat and superabsorbent resin at a weight ratio of 40-50:40-50:0.5-1;

[0031] After the perlite, peat and superabsorbent resin are mixed, they need to be placed in an oven for dry heat sterilization at 130 DEG C for 2 hours, and then sterile water is sprayed to adjust the humidity;

[0032] The weight ratio of the perlite, peat and super absorbent resin is preferably 43-47:43-47:0.7-0.8, and further preferably 45:45:0.75;

[0033] The underground shelter and moisture layer provides an extremely stable underground shelter for the larva molting and dormancy; the excellent water retention and acidic bacteriostatic environment of the peat, the excellent air permeability of the perlite, and the addition of the SAP which can efficiently absorb and lock the excess water and slowly release it when the environment is dry, like a "micro-reservoir", the three together build a stable and healthy underground micro-ecological environment, the humidity fluctuation range can be controlled within ±2%, providing an extremely stable underground shelter for the larva molting and dormancy, greatly promoting the smooth molting and healthy growth of the larva.

[0034] In the present application, the laying thickness of the underground shelter and moisture layer is 2.5-3.5 cm, preferably 2.8-3.2 cm, and further preferably 3.0 cm; the humidity of the underground shelter and moisture layer is 50-60%, preferably 53-57%, and further preferably 55%.

[0035] In the present application, the artificial feed slow-release layer comprises artificial feed slow-release blocks.

[0036] The artificial feed slow-release blocks slowly release nutrients under the action of substrate humidity and can maintain their shape; avoiding the problems of powder or small block feed being easily contaminated, hardened and fermented in a humid environment. The larvae will drill and eat the slow-release blocks from the first layer downwards, which is more similar to their behavior of gnawing plant roots in nature; this design greatly reduces the frequency of feed replacement, reduces labor costs and disturbs the opening.

[0037] In the present application, the artificial feed slow-release blocks are obtained by mixing artificial feed and sodium alginate at a mass-volume ratio of 1g:0.8-1.2ml and then cross-linking and solidifying;

[0038] The mass-volume ratio of the artificial feed and sodium alginate is preferably 1g:0.9-1.1ml, and further preferably 1g:1ml;

[0039] The method for cross-linking and solidifying is that the artificial feed and sodium alginate are mixed, then injected into a mold, and cross-linked and solidified with a calcium chloride solution;

[0040] Before mixing, the artificial feed needs to be sterilized by cobalt-60 radiation (8kGy dose);

[0041] The artificial feed slow-release blocks need to be immersed in a 0.5% hypochlorous acid solution for 1min for disinfection before being put in, rinsed with sterile water and dried.

[0042] In the present application, the initial concentration of sodium alginate is 3-7 wt%, preferably 4-6 wt%, and further preferably 5 wt%.

[0043] In the present application, the artificial feed comprises the following components by weight:

[0044] Knoxia valericata powder 15-25 parts, preferably 18-22 parts, and further preferably 20 parts;

[0045] Radix Astragali rhizome powder 10-20 parts, preferably 13-17 parts, and further preferably 15 parts;

[0046] Carrot powder 5-15 parts, preferably 8-12 parts, and further preferably 10 parts;

[0047] Wheat bran 5-15 parts, preferably 8-12 parts, and further preferably 10 parts;

[0048] Silkworm chrysalis powder 5-10 parts, preferably 8-12 parts, and further preferably 10 parts;

[0049] Yeast powder 3-8 parts, preferably 5-6 parts, and further preferably 5.5 parts;

[0050] Sucrose 2-5 parts, preferably 3-4 parts, and further preferably 3.5 parts;

[0051] Curcumin 0.1-0.5 parts, preferably 0.2-0.4 parts, and further preferably 0.3 parts;

[0052] Compound vitamins 0.5-1.5 parts, preferably 0.8-1.2 parts, and further preferably 1 part;

[0053] Mineral salts 0.5-1.5 parts, preferably 0.8-1.2 parts, and further preferably 1 part;

[0054] Nipagin ethyl 0.05-0.15 parts, preferably 0.08-0.12 parts, and further preferably 0.1 parts;

[0055] Water 35-45 parts, preferably 38-42 parts, and further preferably 40 parts.

[0056] In the present application, the surface humus active layer is made by mixing coconut coir, perlite, composted oak leaf powder, and vermicompost in a weight ratio of 50-60:20-30:15-20:5-10, and an observation hole is reserved;

[0057] After mixing coconut coir, perlite, composted oak leaf powder, and vermicompost, they need to be placed in a 65°C oven for indirect pasteurization (4h each time, 12h interval, repeated twice), and then sprayed with sterile water to adjust the humidity;

[0058] The weight ratio of the coconut husk, perlite, rotten oak leaf powder and earthworm manure is preferably 53-57:23-27:17-18:7-8, and further preferably 55:25:17.5:7.5;

[0059] The diameter of the observation hole is 0.8-1.2 cm, preferably 0.9-1.1 cm, and further preferably 1 cm;

[0060] The loose structure of the surface humus active layer simulates the natural surface, induces the larvae to move and forage (feed) on the surface of the layer, and discharges excrement, and the porosity ensures good air permeability and avoids mold caused by water vapor accumulation in the lower layer; at the same time, the layer separates the larvae from the feed in the lower layer, forcing the larvae to actively forage on the surface, facilitating observation of foraging and cleaning of residual feed, and solving the pain points of feed contamination and difficulty in cleaning.

[0061] In the present application, the laying thickness of the surface humus active layer is 2-3 cm, preferably 2.3-2.7 cm, and further preferably 2.5 cm;

[0062] The humidity of the surface humus active layer is 30-40%, preferably 33-37%, and further preferably 35%.

[0063] In the present application, the dynamic feeding method of the artificial feed slow-release block is: initially laying 2.0-3.0 g of the artificial feed slow-release block; during the feeding process, the consumption is checked regularly, and when the consumption rate reaches 40-50%, 0.5-1.5 g of the artificial feed slow-release block is fed through the observation hole;

[0064] The amount of the initially laid artificial feed slow-release block is preferably 2.3-2.7 g, and further preferably 2.5 g;

[0065] The consumption rate is preferably 43-47%, and further preferably 55%; and the supplementary feeding amount is preferably 0.8-1.2 g, and further preferably 1 g.

[0066] The technical solutions provided by the present application will be described in detail below in conjunction with the examples, but they should not be understood as limiting the scope of protection of the present application.

[0067] Example 1: A method for artificially breeding Ophiocordyceps sinensis host bat moth larvae

[0068] S1. In a separate chamber, three layers are constructed in order from bottom to top:

[0069] Underground shelter and moisture layer: mix perlite, peat and super absorbent resin in a weight ratio of 45:45:0.75, after mixing, place in an oven at 130°C for dry heat sterilization for 2h, after cooling, spray sterile water to adjust the humidity to 55%, then lay, the laying thickness is 3cm;

[0070] Artificial feed slow-release layer: mix pearl barley powder 20g, radix astragali stem powder 10g, carrot powder 15g, wheat bran 8g, silkworm chrysalis powder 7g, yeast powder 8g, sucrose 3g, curcumin 0.3g, compound vitamin 1g, mineral salt 1g, nipagin ethyl 0.1g, water 40g, after mixing, sterilize by cobalt-60 radiation (8kGy dose); then mix the artificial feed and 5wt% sodium alginate at a mass-volume ratio of 1g:1ml, inject into the mold, cross-link and solidify with calcium chloride solution to obtain artificial feed slow-release blocks, immerse the artificial feed slow-release blocks in 0.5% hypochlorous acid solution for 1min, rinse with sterile water and dry, then lay, the initial laying amount is 2.5g;

[0071] Surface humus active layer: mix coconut husk, perlite, rotten oak leaf powder and earthworm manure in a weight ratio of 55:25:15:10, then place in a 65°C oven for indirect pasteurization (4h each time, interval 12h, repeat 2 times), spray sterile water to adjust the humidity to 35%, then lay, the laying thickness is 2.5cm, and a 1cm diameter observation hole is reserved;

[0072] S2. The bat moth larvae are individually fed in the small chambers of the joint culture plate, and the size of the small chamber is 6x6x10cm;

[0073] S3. Environmental control: the temperature is 15°C, and the humidity is 80%;

[0074] S4. Feeding management: the consumption of the artificial feed slow-release blocks is regularly detected through the observation hole, when the consumption rate reaches 45%, 1g of artificial feed slow-release blocks is put into the observation hole for feeding, and the feces is regularly cleaned once a week.

[0075] Example 2: A method for artificially breeding host bat moth larvae of Cordyceps sinensis

[0076] S1. Construct a three-layer structure in the independent small chamber from bottom to top:

[0077] Underground shelter and moisture layer: mix perlite, peat and super absorbent resin in a weight ratio of 40:50:0.5, after mixing, place in an oven at 130°C for dry heat sterilization for 2h, after cooling, spray sterile water to adjust the humidity to 50%, then lay, the laying thickness is 2.5cm;

[0078] Artificial feed slow-release layer: 25 g of Polygonum viviparum powder, 10 g of Astragalus root stem powder, 5 g of carrot powder, 5 g of wheat bran, 5 g of silkworm chrysalis powder, 3 g of yeast powder, 2 g of sucrose, 0.1 g of curcumin, 0.5 g of compound vitamins, 0.5 g of mineral salts, 0.05 g of nipagin ethyl, and 35 g of water were mixed and sterilized by cobalt-60 radiation (8 kGy dose); then the artificial feed and 5 wt% sodium alginate were mixed at a mass-volume ratio of 1 g:0.8 ml, injected into a mold, cross-linked and solidified with a calcium chloride solution to obtain artificial feed slow-release blocks, which were immersed in a 0.5% hypochlorous acid solution for 1 min, rinsed with sterile water and dried, and then laid out with an initial laying amount of 2 g;

[0079] Surface humus active layer: coconut coir, perlite, rotten oak leaf powder and earthworm manure were mixed at a weight ratio of 50:30:15:4, then placed in a 65°C oven for indirect pasteurization (4 h each time, 12 h interval, repeated twice), sprayed with sterile water to adjust the humidity to 300%, and then laid out with a laying thickness of 2.5 cm and an observation hole with a diameter of 0.8 cm;

[0080] S2. The bat moth larvae were individually reared in the small chambers of the joint culture plate, and the size of the small chamber was 5x5x8 cm;

[0081] S3. Environmental control: the temperature was 16°C and the humidity was 75%;

[0082] S4. Feeding management: the consumption of the artificial feed slow-release blocks was regularly detected through the observation hole, and when the consumption rate reached 40%, 1.2 g of artificial feed slow-release blocks were added through the observation hole; the feces were cleaned once a week.

[0083] Example 3: A method for artificially breeding host bat moth larvae of Ophiocordyceps sinensis

[0084] S1. A three-layer structure was constructed in the independent small chamber from bottom to top:

[0085] Underground shelter and moisture retention layer: perlite, peat and superabsorbent resin were mixed at a weight ratio of 47:47:0.7, then placed in an oven for dry heat sterilization at 130°C for 2 h, sprayed with sterile water to adjust the humidity to 57% after cooling, and then laid out with a laying thickness of 3.2 cm;

[0086] Artificial feed slow-release layer: 22 g of Polygonum viviparum powder, 17 g of Astragalus root stem powder, 12 g of carrot powder, 12 g of wheat bran, 12 g of silkworm chrysalis powder, 6 g of yeast powder, 4 g of sucrose, 0.4 g of curcumin, 1.2 g of compound vitamins, 1.2 g of mineral salts, 0.12 g of nipagin, and 42 g of water were mixed, and then sterilized by Co-60 radiation (8 kGy dose); then the artificial feed and 6 wt% sodium alginate were mixed at a mass-volume ratio of 1 g:1.1 ml, injected into a mold, crosslinked and solidified with a calcium chloride solution to obtain artificial feed slow-release blocks, which were immersed in a 0.5% hypochlorous acid solution for 1 min, rinsed with sterile water and dried, and then laid out, with an initial laying amount of 2.7 g;

[0087] Surface humus active layer: coconut coir, perlite, rotten oak leaf powder and earthworm manure were mixed at a weight ratio of 57:23:18, then placed in a 65°C oven for indirect pasteurization (4 h each time, 12 h interval, repeated twice), sprayed with sterile water to adjust the humidity to 37%, and then laid out with a laying thickness of 2.7 cm and an observation hole with a diameter of 1.2 cm was prepared;

[0088] S2. The bat larvae were individually reared in the small chambers of the connected culture plates, and the size of the small chambers was 6x6x10 cm;

[0089] S3. Environmental control: the temperature was 14°C and the humidity was 82%;

[0090] S4. Feeding management: the consumption of the artificial feed slow-release blocks was regularly detected through the observation hole, and when the consumption rate reached 50%, 0.8 g of artificial feed slow-release blocks were added through the observation hole; the feces were cleaned once a week.

[0091] Comparative Example 1

[0092] In Reference Example 1, only the artificial feed slow-release layer and the surface humus active layer were constructed, and the underground shelter and moisture retention layer was removed, and the other steps were the same as in Example 1.

[0093] Comparative Example 2

[0094] In Reference Example 1, the component ratio of the underground shelter and moisture retention layer was replaced by perlite and peat = 45:45 (without superabsorbent resin), and the other steps were the same as in Example 1.

[0095] Comparative Example 3

[0096] In Reference Example 1, the artificial feed was prepared into a powdery form according to the formula of Example 1 (not crosslinked with sodium alginate), and was initially added at 2.5 g, and was supplemented at 1 g every 3 days (because the powdery form is easy to be hardened, it needs to be replaced frequently), and the other steps were the same as in Example 1.

[0097] Comparative Example 4

[0098] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0099] Comparative Example 5

[0100] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0101] Comparative Example 6

[0102] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0103] Comparative Example 7

[0104] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0105] Comparative Example 8

[0106] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0107] Comparative Example 9

[0108] Reference Example 1, the artificial feed was replaced with an equal amount of carrot powder, and the other steps were the same as Example 1.

[0109] Experimental Example

[0110] Healthy, uniform 2nd instar larvae of the bat moth were selected and randomly divided into 12 groups (Example 1-3, Comparative Examples 1-9), with 30 larvae per group, and repeated 3 times. The larvae were reared according to the methods described in Examples 1-3 and Comparative Examples 1-9. The rearing period was 150 days, and the number of surviving larvae, body weight, body length, and the number of successfully molted larvae were measured every 30 days. The average final body weight of the larvae, the weight gain rate of the larvae, the survival rate, and the successful molting rate of the larvae after 150 days were measured, and the results are shown in Table 1.

[0111] The weight gain rate (%) = (final body weight - initial body weight) / initial body weight x 100%; the survival rate (%) = (final surviving number of larvae / initial number of larvae) x 100%; and the molting success rate (%) = (number of successfully molted larvae / total number of larvae) x 100%.

[0112] Table 1: Results of index detection

[0113]

[0114] As can be seen from Table 1, the survival rate of bat moth larvae can reach more than 85% by using the method of the present application to feed the bat moth larvae, wherein the survival rate can reach 92.2% by using the method of Example 1, the ecdysis success rate is 94.3%, and the weight gain rate is 48.0%. This shows that the feeding method provided by the present application can effectively promote the healthy growth of bat moth larvae, significantly improve the survival rate, growth rate and ecdysis success rate of bat moth larvae, and provide high-quality host larvae for artificial cultivation of Cordyceps sinensis.

[0115] As can be seen from the above examples, the present application provides a method for artificially feeding host bat moth larvae of Cordyceps sinensis, which can significantly improve the survival rate and health of bat moth larvae, shorten the growth cycle, and facilitate large-scale management.

[0116] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the protection scope of the present application.

Claims

1. A method for artificially rearing the larvae of the ghost moth, a host plant of Cordyceps sinensis, characterized in that... Includes the following steps: S1. Three layers are constructed from bottom to top in an independent small room: an underground shelter and moisture-retaining layer, an artificial feed slow-release layer, and a surface humus activity layer. S2. Individual bat moth larvae are reared in a small room; S3. Environmental control: Temperature 10-18℃, humidity 75-85%; S4. Feeding and management: Dynamically feed artificial feed with slow release and clean up feces.

2. The method according to claim 1, characterized in that, The underground shelter and moisture-retaining layer is made of perlite, peat and superabsorbent resin in a weight ratio of 40-50:40-50:0.5-1.

3. The method according to claim 2, characterized in that, The thickness of the underground protective moisture-retaining layer is 2.5–3.5 cm; the humidity of the underground protective moisture-retaining layer is 50–60%.

4. The method according to claim 3, characterized in that, The artificial feed slow-release layer includes artificial feed slow-release blocks.

5. The method according to claim 4, characterized in that, The artificial feed slow-release block is obtained by cross-linking and solidifying artificial feed and sodium alginate mixed at a mass-volume ratio of 1g:0.8-1.2ml.

6. The method according to claim 5, characterized in that, The initial concentration of sodium alginate is 3–7 wt%.

7. The method according to claim 6, characterized in that, The artificial feed comprises the following components in parts by weight: 15-25 parts of Polygonum bulbiferum powder, 10-20 parts of Astragalus membranaceus root and rhizome powder, 5-15 parts of carrot powder, 5-15 parts of wheat bran, 5-10 parts of silkworm pupa powder, 3-8 parts of yeast powder, 2-5 parts of sucrose, 0.1-0.5 parts of curcumin, 0.5-1.5 parts of compound vitamins, 0.5-1.5 parts of mineral salts, 0.05-0.15 parts of ethylparaben, and 35-45 parts of water.

8. The method according to claim 7, characterized in that, The surface humus activity layer is made of coconut coir, perlite, decomposed oak leaf powder and earthworm castings in a weight ratio of 50-60:20-30:15-20:5-10, and observation holes are reserved.

9. The method according to claim 8, characterized in that, The thickness of the surface humic active layer is 2-3 cm; the humidity of the surface humic active layer is 30-40%.

10. The method according to claim 9, characterized in that, The dynamic feeding method for the artificial feed slow-release blocks is as follows: initially lay out 2.0-3.0g of artificial feed slow-release blocks; during the feeding process, check the consumption regularly, and when the consumption rate reaches 40%-50%, add 0.5-1.5g of slow-release blocks through the observation hole.

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