Pitaya canker prevention and treatment preparation and preparation method and use method thereof

Abstract

The invention discloses an emulsion in water for preventing and treating dragon fruit canker as well as a preparation method and a use method thereof. The emulsion in water consists of the following components: laurinol, chlorogenic acid, a surfactant, a thickening agent, an emulsifier, a co-emulsifier and water. The preparation process comprises oil phase emulsification and water phase mixing. When in use, the composition is diluted by 500 times and is uniformly sprayed on the surfaces of pitaya plants, and the composition is sprayed once every 7 days and is continuously sprayed twice as a cycle. The preparation forms a dual prevention and control system of film-forming permeation stress resistance and sterilization repair through the synergistic effect of stress resistance of chlorogenic acid and bacteriostasis, sterilization and permeation enhancement of laurinol, has the advantages of efficient bacteriostasis, low toxicity, environmental protection, high stability (good fluidity after freezing / cold storage), controllable cost and the like, and is suitable for green prevention and control of dragon fruit canker.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant disease and pest control in agriculture, and particularly relates to a water emulsion for preventing and treating dragon fruit canker disease, and a preparation and use method thereof. BACKGROUND

[0002] Dragon fruit is a tropical and subtropical fruit with high economic value and is widely planted in southern China. However, dragon fruit canker disease is one of the important diseases that harm the growth of dragon fruit. The disease is caused by fungi (such as Colletotrichum fungi and Phomopsis fungi), mainly invades dragon fruit plants through wounds, stomata and the like, causes round or oval lesions on the stems and fruits of the plants, and the lesions are concave and accompanied by gum flow, which seriously affects the yield and quality of dragon fruit and brings huge economic losses to the growers.

[0003] At present, the methods for preventing and treating dragon fruit canker disease on the market mainly include chemical control, biological control and agricultural control. Chemical control relies on a large amount of chemical pesticides (such as carbendazim and thiophanate-methyl), which is easy to cause fungal resistance and pesticide residue problems; the effect of biological control is unstable; and the effect of agricultural control (such as pruning and ventilation) is limited. Therefore, it is a technical problem to be solved in the field to develop a prevention and treatment agent with high efficiency, environmental friendliness and economic efficiency. SUMMARY

[0004] The purpose of the present application is to provide a water emulsion for preventing and treating dragon fruit canker disease, which is efficient in inhibiting bacteria, low in toxicity, environmentally friendly, stable and controllable in cost. Through scientific formula and process, the water emulsion can achieve precise prevention and control of the disease and reduce the dependence on chemical pesticides.

[0005] According to one aspect of the present application, a water emulsion for preventing and treating dragon fruit canker disease is provided, which is composed of the following components in terms of weight ratio: lauryl alcohol 300-500 parts, chlorogenic acid 50-200 parts, surfactant 50-200 parts, thickening agent 10-30 parts, emulsifier 5-10 parts, co-emulsifier 1-5 parts, and water 2000-3000 parts.

[0006] Further, chlorogenic acid is the main component and has the effects of enhancing plant stress resistance and promoting plant growth, and can enhance the disease resistance of plants through double mechanisms.

[0007] Further, lauryl alcohol can improve the permeability and wettability of the preparation, has the effects of inhibiting and killing bacteria, and cooperates with chlorogenic acid to inhibit fungi through the penetration enhancement effect, thereby improving the bactericidal repair ability of the preparation.

[0008] Further, tea saponin or sucrose fatty acid ester as a surfactant, helps to improve the emulsification, dispersion and wetting properties of the preparation, so that other ingredients are better mixed and attached to the plant surface, while having inhibitory and killing effects on bacteria, fungi and various pests.

[0009] Further, the thickening agent is hydroxypropyl methyl cellulose or hydroxyethyl cellulose.

[0010] Further, the emulsifier is a mixture of one or more of non-ionic surfactants Tween 20, Tween 80, high carbon fatty alcohol polyoxyethylene ether, fatty acid polyoxyethylene ester, sorbitan ester, used to form a stable oil-in-water system.

[0011] Further, the co-emulsifier is n-propanol or n-butanol, which plays a role in solubilization and adjusting the physical properties of the preparation.

[0012] According to another aspect of the present application, the preparation method of the above-mentioned water emulsion for preventing and treating pitaya canker is also provided, comprising the following steps:

[0013] 1. Oil phase preparation: mix lauryl alcohol, emulsifier AEO6, and co-emulsifier at 60°C, homogenize at 10000 r / min for 2 min to form a stable oil phase.

[0014] 2. Primary emulsification: slowly add the surfactant to the oil phase, keep the oil phase at 60°C, maintain the speed at 10000 rpm, and continue homogenization until the system is evenly emulsified.

[0015] 3. Water phase mixing: dissolve chlorogenic acid in 40°C water, stir evenly, then slowly drop into the emulsion system, maintain the speed at 10000 rpm, and continue homogenization until the system is evenly emulsified.

[0016] 4. Product preparation: add the thickening agent, maintain the speed at 10000 rpm, continue to rotate for 30 min, slowly stir in a water bath to cool down, and obtain the water emulsion.

[0017] According to another aspect of the present application, the use method of the above-mentioned water emulsion for preventing and treating pitaya canker is also provided: dilute the water emulsion 500 times, evenly spray on the surface of pitaya plants to form a bacteriostatic film, block the transmission route of fungi; spray once every 7 days, continuously for 2 times as a cycle, and the dosage is adjusted according to the size of the plant and the degree of disease.

[0018] The water-in-oil emulsion of this invention can achieve the technical effects of cutting off the main transmission route of dragon fruit canker, preventing the spread of canker, inhibiting the pathogenic fungi of dragon fruit canker, and promoting the recovery of diseased trees. Specifically, this is achieved through the following methods: the water-in-oil emulsion is diluted 500 times and sprayed on the fruit trees, which can form a film on the plant surface, blocking fungal spores and inhibiting the spread of pests, thus cutting off the transmission route of canker. Lauryl alcohol carries chlorogenic acid deep into the tissue through osmosis, jointly activating the plant's resistance to enhance stress resistance. Lauryl alcohol destroys the fungal cell membrane to achieve bacteriostatic and bactericidal effects. At the same time, the surfactant enhances the adhesion of the liquid and plays an insecticidal and disease-preventing role.

[0019] The beneficial effects of this invention are as follows:

[0020] 1. Highly efficient dual control: The water-in-oil emulsion of this invention uses chlorogenic acid and lauryl alcohol as core components to achieve highly efficient control through the synergistic effect of "stress resistance + antibacterial action": chlorogenic acid induces resistance proteins to enhance plant stress resistance and indirectly inhibits fungi; lauryl alcohol has both antibacterial and penetrating functions, directly killing pathogens and helping chlorogenic acid penetrate deep into tissues; the two work synergistically with surfactants to enhance the adhesion of the pesticide solution, and the spraying forms an antibacterial film to cut off the transmission routes of thrips and other pathogens.

[0021] 2. Environmentally friendly and safe: It is mainly composed of natural / low-toxic ingredients, with no chemical pesticide residues, and is safe for the environment, human body and beneficial organisms, meeting the standards of green agriculture.

[0022] 3. Strong stability: The formulation maintains good fluidity after freezing / refrigeration, returns to its original state after thawing, and shows no obvious stratification after standing for 30 days, meeting the requirements for long-term storage and transportation.

[0023] 4. Low cost: Raw materials are readily available (such as chlorogenic acid, lauryl alcohol, surfactants, etc.), the process is simple, the production cost is low, and it is suitable for large-scale production and promotion. Attached Figure Description

[0024] Figure 1 Comparison of dragon fruit before and after treatment in experimental group 1

[0025] Figure 2 Comparison of dragon fruit before and after treatment in experimental group 2

[0026] Figure 3 Comparison of dragon fruit before and after treatment in experimental group 3

[0027] Figure 4 Comparison of dragon fruit before and after treatment in the control group Detailed Implementation

[0028] The present invention will be described in detail below through specific embodiments, but the scope of protection of the present invention is not limited thereto.

[0029] Example 1

[0030] This invention provides a water-in-oil emulsion for preventing and treating dragon fruit canker, which is composed of the following components by weight: 400 parts lauryl alcohol, 50 parts chlorogenic acid, 80 parts surfactant, 10 parts thickener, 5 parts emulsifier, 2 parts co-emulsifier, and 2800 parts water.

[0031] In this embodiment, the surfactant is tea saponin. The thickener is hydroxyethyl cellulose. The emulsifier is high-carbon fatty alcohol polyoxyethylene ether. The co-emulsifier is n-propanol.

[0032] This embodiment also provides a method for preparing the above-mentioned water-in-oil emulsion for preventing and treating dragon fruit canker, including the following steps:

[0033] 1. Oil phase preparation: 400 parts lauryl alcohol, 5 parts emulsifier, and 2 parts co-emulsifier were mixed at 60℃ and homogenized at 10000r / min for 2min to form a stable oil phase.

[0034] 2. Initial emulsification: Slowly add 80 parts of surfactant to the oil phase, keep the oil phase at 60°C, maintain a rotation speed of 10,000 rpm, and continue homogenizing until the system is uniformly emulsified.

[0035] 3. Aqueous phase mixing: Dissolve 50 parts of chlorogenic acid in 2800 parts of water at 40℃, stir well, and then slowly drip it into the emulsion system while maintaining a rotation speed of 10000 rpm, continuing to homogenize until the system is uniformly emulsified.

[0036] 4. To obtain the finished product: Add 10 parts of thickener, maintain a rotation speed of 10,000 rpm, continue rotating for 30 minutes, and then slowly stir and cool in a water bath to obtain the water emulsion.

[0037] Example 2

[0038] This invention provides a water-in-oil emulsion for preventing and treating dragon fruit canker, which is composed of the following components by weight: 350 parts lauryl alcohol, 100 parts chlorogenic acid, 100 parts surfactant, 10 parts thickener, 8 parts emulsifier, 2 parts co-emulsifier, and 2800 parts water.

[0039] In this embodiment, the surfactant is a sucrose fatty acid ester. The thickener is hydroxyethyl cellulose. The emulsifier is a mixture of Tween 20 and higher carbon fatty alcohol polyoxyethylene ether. The co-emulsifier is n-butanol.

[0040] This embodiment also provides a method for preparing the above-mentioned water-in-oil emulsion for preventing and treating dragon fruit canker, including the following steps:

[0041] 1. Oil phase preparation: 350 parts lauryl alcohol, 8 parts emulsifier, and 2 parts co-emulsifier were mixed at 60℃ and homogenized at 10000r / min for 2min to form a stable oil phase.

[0042] 2. Initial emulsification: Slowly add 100 parts of surfactant to the oil phase, keep the oil phase at 60°C, maintain a rotation speed of 10,000 rpm, and continue homogenizing until the system is uniformly emulsified.

[0043] 3. Aqueous phase mixing: Dissolve 100 parts of chlorogenic acid in 2800 parts of water at 40℃, stir evenly, and slowly drip into the emulsion system while maintaining a rotation speed of 10000 rpm and continuing to homogenize until the system is uniformly emulsified.

[0044] 4. To obtain the finished product: Add 10 parts of thickener, maintain a rotation speed of 10,000 rpm, continue rotating for 30 minutes, and then slowly stir and cool in a water bath to obtain the water emulsion.

[0045] Example 3

[0046] This invention provides a water-in-oil emulsion for preventing and treating dragon fruit canker, which is composed of the following components by weight: 300 parts lauryl alcohol, 200 parts chlorogenic acid, 150 parts surfactant, 10 parts thickener, 10 parts emulsifier, 2 parts co-emulsifier, and 2800 parts water.

[0047] In this embodiment, the surfactant is tea saponin. The thickener is hydroxyethyl cellulose. The emulsifier is a mixture of Tween 20 and high-carbon fatty alcohol polyoxyethylene ether. The co-emulsifier is n-propanol.

[0048] This embodiment also provides a method for preparing the above-mentioned water-in-oil emulsion for preventing and treating dragon fruit canker, including the following steps:

[0049] 1. Oil phase preparation: 300 parts lauryl alcohol, 10 parts emulsifier and 2 parts co-emulsifier were mixed at 60℃ and homogenized at 10000r / min for 2min to form a stable oil phase.

[0050] 2. Initial emulsification: Slowly add 150 parts of surfactant to the oil phase, keep the oil phase at 60°C, maintain a rotation speed of 10,000 rpm, and continue homogenizing until the system is uniformly emulsified.

[0051] 3. Aqueous phase mixing: Dissolve 200 parts of chlorogenic acid in 2800 parts of water at 40℃, stir evenly, and slowly drip into the emulsion system while maintaining a rotation speed of 10000 rpm and continuing to homogenize until the system is uniformly emulsified.

[0052] 4. To obtain the finished product: Add 10 parts of thickener, maintain a rotation speed of 10,000 rpm, continue rotating for 30 minutes, and then slowly stir and cool in a water bath to obtain the water emulsion.

[0053] Example 4 (How to use)

[0054] 1. This embodiment provides a method for using a water-in-oil emulsion to prevent and treat dragon fruit canker: When using the water-in-oil emulsion prepared in Embodiments 1, 2 or 3 of this invention, dilute the water-in-oil emulsion with water 500 times and spray it evenly on the dragon fruit plants; spray once every 7 days, and two consecutive times constitute one cycle. The dosage is adjusted according to the size of the plant and the severity of the disease.

[0055] Example 5 (Homogeneity Test)

[0056] Take 5 mL of each of the water emulsions prepared in Examples 1-3, place them in centrifuge tubes, centrifuge at 8000 r / min for 2 minutes, and observe the state of the system.

[0057] Results: No stratification, precipitation or oil-water separation was observed in any of the samples after centrifugation. The system remained uniform and stable, and all formed homogeneous solutions.

[0058] Conclusion: The water-in-oil emulsions prepared in Examples 1-3 of this invention have good homogeneity and meet the requirements for formulation stability.

[0059] Example 6 (Stability Test)

[0060] Take appropriate amounts of each of the water-in-oil emulsions prepared in Examples 1-3, and subject them to freezing (-20℃, 24h), refrigeration (4℃, 7 days), and standing at room temperature (30 days) treatments, respectively, and observe the changes in their state.

[0061] Results: After freezing, thawing, refrigeration, and standing, no stratification or sedimentation was observed; the fluidity was good, and the product returned to its original state.

[0062] Conclusion: The water-in-oil emulsions prepared in Examples 1-3 of this invention have strong stability and meet the requirements for storage and transportation.

[0063] Example 7 (Film-forming and Repair Capability Test)

[0064] Take the water-based emulsions prepared in Examples 1-3, dilute them 500 times and 1000 times respectively, and place 1 drop of each on a glass slide. Let them air dry to form a plant protection film. Make 3-5 cracks (about 0.5 mm wide) with a scalpel, add 1 drop of distilled water to the cracks, and observe the repair.

[0065] Results: Both 500-fold and 1000-fold diluted water-in-oil emulsions formed continuous, uniform, transparent films on the glass slides. After adding water, the cracks gradually contracted and closed within 30 seconds, and were essentially repaired within 1 minute, restoring the film surface to its original integrity.

[0066] Conclusion: The water emulsions prepared in Examples 1-3 of this invention exhibit good film-forming properties after dilution, and the film structure has excellent water-sensitive repair capabilities.

[0067] Example 8 (Prevention and Control Efficacy Test)

[0068] 1. Prevention and control trial protocol

[0069] Experimental location: Dragon fruit plantation in Li Autonomous County, Hainan Province

[0070] Timing: Peak season for peptic ulcer disease (May-June)

[0071] Test reagents:

[0072] ●Sample A: Water-based emulsion prepared in Example 1

[0073] ●Sample B: Water-based emulsion prepared in Example 2

[0074] ●Sample C: Water-based emulsion prepared in Example 3

[0075] ●Control group: Water

[0076] Test method:

[0077] ●Experimental Groups 1-3: Samples A, B, and C were diluted 500 times with water and sprayed twice, with 100 dragon fruit trees in each group;

[0078] ●Control group: Sprayed with equal amount of water twice, 100 dragon fruit trees;

[0079] Operation details:

[0080] ● Spraying method: Spray evenly on the surface of the dragon fruit plant until it drips.

[0081] ●Prevention and control cycle: Treat once every 7 days, for a total of 2 times.

[0082] 2. Comparison Table of Prevention and Control Effects

[0083]

[0084]

[0085] Summary of control effects: The water-in-oil emulsions of experimental groups 1 and 2 can effectively inhibit the development of dragon fruit canker, especially experimental group 1, which has outstanding repair ability; Although the water-in-oil emulsion of experimental group 3 is less effective, it can still delay the deterioration of the disease compared with the control group; The control group shows that if no effective measures are taken, dragon fruit canker will spread rapidly and lead to rot.

[0086] The present invention relates to a dragon fruit canker control agent and its preparation and application method. Through reasonable formulation design and scientific preparation and application procedures, it can effectively solve the problems in the prevention and control of dragon fruit canker, providing strong support for the healthy development of the dragon fruit industry. In practical applications, the formulation and application method can be appropriately adjusted according to different dragon fruit varieties, growing environments, and disease severity to achieve the best control effect. Furthermore, conventional improvements and equivalent substitutions made by those skilled in the art based on this invention should all fall within the scope of protection of this invention.

Claims

1. A water-based emulsion for preventing and treating dragon fruit canker, characterized in that, The water-based emulsion is composed of the following components: lauryl alcohol, chlorogenic acid, surfactant, emulsifier, co-emulsifier, and water.

2. The water-in-oil emulsion for preventing and treating dragon fruit canker according to claim 1, characterized in that, The composition by weight is as follows: 300-500 parts lauryl alcohol, 50-200 parts chlorogenic acid, 50-200 parts surfactant, 10-30 parts thickener, 5-10 parts emulsifier, 1-5 parts co-emulsifier, and 2000-3000 parts water.

3. The water-in-oil emulsion for preventing and treating dragon fruit canker according to claim 2, characterized in that, The surfactant is tea saponin or sucrose fatty acid ester, used to improve the emulsifying, dispersing and wetting properties of the formulation, so that other components can be better mixed and adhered to the plant surface.

4. The water-in-oil emulsion for preventing and treating dragon fruit canker according to claim 3, characterized in that, The thickener is hydroxypropyl methylcellulose or hydroxyethyl cellulose.

5. The water-in-oil emulsion for preventing and treating dragon fruit canker according to claim 4, characterized in that, The emulsifier is a nonionic surfactant, including one or more of Tween 20, Tween 80, high carbon fatty alcohol polyoxyethylene ether, fatty acid polyoxyethylene ester, and sorbitan ester.

6. The water-in-oil emulsion for preventing and treating dragon fruit canker according to claim 5, characterized in that, The emulsifier is n-propanol or n-butanol, used to adjust the solubility and physical stability of the formulation.

7. A method for preparing the water-in-oil emulsion for preventing and treating dragon fruit canker as described in claim 6, characterized in that, The preparation method includes the following steps: (1) Preparation of oil phase: Lauryl alcohol, emulsifier AEO6 and co-emulsifier are mixed at 60℃ and homogenized at 10000r / min for 2min to form a stable oil phase. (2) Initial emulsification: The surfactant is slowly added to the oil phase, the oil phase is kept at 60°C, the rotation speed is kept at 10,000 rpm, and the mixture is continuously homogenized until the system is uniformly emulsified. (3) Aqueous phase mixing: Dissolve chlorogenic acid in water at 40°C, stir evenly, and slowly drip it into the emulsion system while maintaining a rotation speed of 10,000 rpm and continuing to homogenize until the system is uniformly emulsified. (4) To obtain the finished product: Add thickener, keep the rotation speed at 10,000 rpm, continue to rotate for 30 minutes, and slowly stir and cool in a water bath to obtain the water emulsion.

8. A method of using the water-in-oil emulsion for preventing and treating dragon fruit canker as described in claim 7, characterized in that: Dilute the emulsion with water 500 times and spray it evenly on the surface of the dragon fruit plant. Spray once every 7 days, and repeat twice as one cycle.

Images