Method for improving germination rate of Leymus chinensis seeds by using plant growth regulator fluridone
By spraying the spikelets of Leymus chinensis with a fluroxypyr solution, the problem of low seed germination rate was solved, achieving more efficient and uniform seed treatment, improving germination rate and saving labor costs.
Patent Information
- Application Number
- CN202511144840.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-15
- Publication Date
- 2025-11-18
AI Technical Summary
Sheepgrass seeds have a low germination rate, and existing pesticide treatment methods are uneven, wasteful, and increase labor costs, thus affecting production efficiency.
Treat the spikes of Leymus chinensis plants with a fluroxypyr solution at a concentration of 100–1000 μmol/L during the grain-filling stage, ensuring the spikes are completely saturated. Subsequent cultivation and management remain unchanged until the seeds mature and are harvested.
Fluroxypyrone solution is evenly distributed on seeds through natural physiological processes, improving seed physiological state, increasing germination rate, simplifying treatment steps, and saving time and labor.
Smart Images
Figure CN120959245A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of sheepgrass cultivation technology, and particularly relates to a method for improving the germination rate of sheepgrass seeds using the plant growth regulator fluazinam. Background Technology
[0002] Leymus chinensis is a perennial grass belonging to the Poaceae family, widely distributed in Northeast, North, and Northwest China, as well as Mongolia and Russia. It is rich in nutrients, has abundant leaves, high crude protein content, and good palatability, making it an important component of temperate grasslands. While Leymus chinensis has significant value in forage grasses and ecological restoration, its slow growth rate and low seed germination rate hinder its widespread application and efficient utilization.
[0003] To improve the germination rate of Leymus chinensis seeds, post-harvest treatments such as soaking, coating, or spraying are commonly performed. However, these methods have limitations. First, post-harvest treatments often fail to evenly cover all seeds, leading to inconsistent effectiveness. Second, pesticide residues on the seed surface may not be fully absorbed, resulting in waste and environmental pollution. Furthermore, post-harvest treatments increase labor and time costs, impacting production efficiency. Therefore, there is an urgent need to develop a more efficient and user-friendly treatment method to address the low germination rate of Leymus chinensis seeds. Summary of the Invention
[0004] In view of this, the purpose of the present invention is to provide a method for improving the germination rate of sheepgrass seeds. This method is simple and can more effectively improve the physiological state and germination potential of the seeds.
[0005] Another object of the present invention is to provide sheepgrass seeds obtained by the method described above.
[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:
[0007] The present invention provides a method for improving the germination rate of Leymus chinensis seeds, the method comprising: treating the spike of Leymus chinensis plants with a fluazinam solution.
[0008] Preferably, the concentration of the fluazinon solution is 100–1000 μmol / L.
[0009] Preferably, the concentration of the fluazinon solution is 200–600 μmol / L.
[0010] Preferably, the treatment is performed during the grouting period.
[0011] Preferably, the treatment is performed 12 to 16 days after flowering.
[0012] Preferably, the treatment is performed 14 days after flowering.
[0013] Preferably, the treatment method includes spraying.
[0014] Preferably, after treatment with fluazinam solution, the cultivation and management conditions of Leymus chinensis remain unchanged, and the seeds are harvested after maturity.
[0015] Preferably, the variety of sheepgrass includes Jisheng No. 4 or Nongmu No. 1.
[0016] The present invention also provides sheepgrass seeds obtained by the method described above.
[0017] The beneficial effects of this invention are:
[0018] This invention provides a method for improving the germination rate of Leymus chinensis seeds. Fluroxypyr solution is applied during spike development, directly acting on the seed development process. This allows the fluroxypyr to be more effectively absorbed and utilized by the seeds, promoting a higher germination rate. Fluroxypyr enters the seed through natural physiological processes, distributing evenly throughout the spike and improving its physiological state during seed formation, thus enhancing subsequent germination rates. No additional post-harvest treatment is required, saving time and labor. Compared to treating seeds directly with chemicals after harvest, this method offers multiple advantages, including more efficient absorption, more uniform distribution, earlier improvement of seed quality, and simplified subsequent steps, effectively enhancing the physiological state and germination potential of the seeds. Attached Figure Description
[0019] Figure 1 The seed germination rate of the six Leymus chinensis samples in Experiment Example 1;
[0020] Figure 2 Seed germination status of L11 sheepgrass material in Experiment Example 1;
[0021] Figure 3 The seed germination rate of the 14 Leymus chinensis materials in Experiment Example 2 is given. Detailed Implementation
[0022] The present invention provides a method for improving the germination rate of Leymus chinensis seeds, the method comprising: treating the spike of Leymus chinensis plants with a fluazinam solution.
[0023] In this invention, the concentration of the fluazindone solution is preferably 100 to 1000 μmol / L, for example, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 μmol / L.
[0024] In this invention, the preferred treatment period is the grain-filling stage; the grain-filling stage is a crucial period in the reproductive growth of Leymus chinensis, referring to the period after fertilization of the inflorescence (spike) and the beginning of seed formation and gradual accumulation of nutrients. In some embodiments, the preferred treatment period is 12–16 days after flowering, for example, 12, 13, 14, 15, or 16 days. Applying fluroxypyrone solution during spike development directly acts on the seed development process, allowing fluroxypyrone to be more effectively absorbed and utilized by the seeds, promoting an increased germination rate; fluroxypyrone enters the seeds through natural physiological processes, can be evenly distributed in the spike, and can improve the physiological state of the seed during the seed formation stage, increasing the subsequent germination rate; no additional treatment is required after harvest, saving time and labor.
[0025] In this invention, the preferred treatment method includes spraying, ensuring that the panicles are completely saturated. In some embodiments, the preferred spraying amount is 10-30 mL / plant, for example, 10, 15, 20, 25, or 30 mL / plant.
[0026] In this invention, after treatment with fluroxypyrone solution, the cultivation and management conditions of Leymus chinensis remain unchanged, and conventional cultivation and management methods are adopted. The seeds are harvested after they mature.
[0027] In this invention, the preferred varieties of sheepgrass include Jisheng No. 4 or Nongmu No. 1.
[0028] The present invention also provides sheepgrass seeds obtained by the method described above.
[0029] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0030] Unless otherwise specified, the following embodiments are all conventional methods.
[0031] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.
[0032] Example 1
[0033] A method for improving the germination rate of Leymus chinensis seeds, comprising the following steps: 14 days after the flowering of Leymus chinensis, spray the spikes of the Leymus chinensis plants with a 600 μmol / L fluazinam solution to ensure that the spikes are completely wetted.
[0034] Example 2
[0035] A method for improving the germination rate of Leymus chinensis seeds, comprising the following steps: 12 days after the flowering of Leymus chinensis, spray the spikes of the Leymus chinensis plants with a 200 μmol / L fluazinam solution to ensure that the spikes are completely wetted.
[0036] Example 3
[0037] A method for improving the germination rate of Leymus chinensis seeds, comprising the following steps: 16 days after the flowering of Leymus chinensis, spray the spikes of the Leymus chinensis plants with a 1000 μmol / L fluazinam solution to ensure that the spikes are completely wetted.
[0038] Experimental Example 1
[0039] 1. Sheepgrass materials
[0040] Six samples of sheepgrass were selected, and the information is shown in Table 1.
[0041] Table 1 Information on Leymus chinensis materials
[0042] Material Number source L1 Collected from Beijing Botanical Garden L2 Fengtun Village, Shizhai Town, Feng County, Xuzhou City, Jiangsu Province L3 Yushutun, Angangxi District, Qiqihar City, Heilongjiang Province L5 Collected from Fuyu County L6 Collected from Fengtun Village, Shizhai Town, Feng County, Xuzhou City, Jiangsu Province L7 Collected from Hulunbuir League L11 "Jisheng No. 4"
[0043] 2. Method:
[0044] Six samples of Leymus chinensis were transplanted into 24cm diameter quadrats, with one plant per quadrat. Fourteen days after flowering, the spikes of each of the six samples were sprayed with different concentrations (200, 400, 600, 800, and 1000 μmol / L) of fluazinam solution, 20 mL per quadrat, ensuring the spikes were completely saturated. Conventional cultivation management methods were followed for the remaining periods. After seed maturity, the seeds were harvested, and a control group was formed by spraying an equal volume of water. FL1-FL5 concentrations were 200, 400, 600, 800, and 1000 μmol / L, respectively, while CK served as the control group.
[0045] After harvesting, the seeds were threshed and germination experiments were conducted. Forty-five days after harvest, five seeds were taken from each treatment concentration of *Leymus chinensis* material and germinated at 28℃ for 12 hours and 16℃ for 12 hours daily. Each treatment concentration was replicated four times. Germination rate was recorded 14 days after germination. The results are shown in Table 2. Figure 1 As shown.
[0046] Table 26: Seed germination rate (%) of Leymus chinensis materials
[0047] CK FL1 FL2 FL3 FL4 FL5 L1 70.625 86.875 88.125 89.375 86.875 94.375 L5 28.75 66.25 39.375 68.75 65.625 60 L3 20.625 58.125 49.375 41.25 85 80.625 L7 44.375 78.75 76.875 66.25 85.625 91.875 L6 36.875 54.375 68.75 81.25 44.375 38.125 L2 9.375 65 63.125 66.875 68.125 73.125
[0048] Depend on Figure 1 As shown in Table 2, two materials (L5 and L6) had high germination rates at a concentration of 600 μmol / L; one material (L3) had a high germination rate at a concentration of 800 μmol / L; and three materials (L1, L7, and L2) had high germination rates at a concentration of 1000 μmol / L.
[0049] Take Leymus chinensis material L11, treat it using the same method as described above, and conduct a germination experiment. Observe and photograph it 14 days after germination. The results are as follows: Figure 2 As shown. By Figure 2The results showed that the germination rate of L11 sheepgrass seeds was significantly increased after treatment with fluazinam solution. Some seedlings had green leaves after treatment with 600 μmol / L fluazinam solution, while all seedlings turned white after treatment with 800 μmol / L and 1000 μmol / L fluazinam solution.
[0050] Experimental Example 2
[0051] 1. Sheepgrass materials
[0052] Fourteen samples of sheepgrass were selected, and the information is shown in Table 3.
[0053] Table 3 Information on Leymus chinensis materials
[0054]
[0055]
[0056] 2. Method:
[0057] Using the same method as in Experiment 1, 14 portions of Leymus chinensis were sprayed with 200 μmol / L (P1) and 600 μmol / L (P3) fluazinam solutions, respectively, with an equal volume of water sprayed as a control group (CK). Seed germination rate was recorded 18 days after germination, and the results are shown in Table 4. Figure 3 As shown.
[0058] Table 4. Germination rate (%) of 114 Leymus chinensis seeds
[0059] L8 L9 L5 L11 L12 L13 L14 L15 L16 L6 L2 L4 L19 L7 CK 40 7 30 36 35 52.5 82.5 77 62.5 20.5 6.5 8 48 18 P1 71 17 25.5 59.5 62 63.5 94.5 93 79 54 27 29.5 80 27.5 P3 62 19.5 48 67.5 70 40.5 92.5 83.5 73 56 41 34.5 84 63
[0060] It can be seen that five of the materials (L8, L13, L14, L15, and L16) had high germination rates when sprayed with a 200 μmol / L fluazinam solution, while the remaining nine materials had the highest germination rates when sprayed with a 600 μmol / L fluazinam solution.
[0061] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for improving the germination rate of sheepgrass seeds, characterized in that, The method includes treating the spikes of sheepgrass plants with a fluroxypyrone solution.
2. The method according to claim 1, characterized in that, The concentration of the fluazinam solution is 100–1000 μmol / L.
3. The method according to claim 2, characterized in that, The concentration of the fluazinon solution is 200–600 μmol / L.
4. The method according to claim 1, characterized in that, The treatment period is the grouting period.
5. The method according to claim 4, characterized in that, The treatment was performed 12–16 days after flowering.
6. The method according to claim 5, characterized in that, The treatment was performed 14 days after flowering.
7. The method according to claim 1, characterized in that, The treatment methods include spraying.
8. The method according to claim 1, characterized in that, After treatment with fluazinam solution, the cultivation and management conditions for Leymus chinensis remained unchanged, and the seeds were harvested after maturity.
9. The method according to claim 1, characterized in that, The varieties of sheepgrass include Jisheng No. 4 or Nongmu No.
1.
10. Sheepgrass seeds obtained by the method according to any one of claims 1 to 9.