Root administration method for preventing and treating tobacco wildfire
By using a root application method involving soaking and drenching roots with copper hydroxide suspension during the tobacco seedling stage and after transplanting, the problem of controlling pathogens with foliar spraying in existing technologies has been solved, achieving highly efficient prevention and control of tobacco wildfire disease.
Patent Information
- Application Number
- CN202511469926.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-15
- Publication Date
- 2025-11-18
AI Technical Summary
In existing technologies, the prevention and control of tobacco wildfire disease mainly relies on foliar spraying, which makes it difficult to control the pathogens at the source, resulting in poor control effects.
A method of applying copper hydroxide suspension to the roots of tobacco seedlings by soaking them in water and then drenching them after transplanting creates a protective layer that prevents pathogens from invading the roots.
By improving the application methods, the incidence of tobacco wildfire disease has been significantly reduced, and the precision and effectiveness of prevention and control have been improved.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of plant disease control, and relates to a method for controlling tobacco wildfire disease, particularly a method for administering medication through the roots to control tobacco wildfire disease. Background Technology
[0002] The tobacco-pathogenic strain of *Pseudomonas tonsillar* ( Pseudomonas amygdali pv. tabaci [Synonyms: *Pseudomonas syringae* spp. (Tobacco-pathogenic strain)] P . syringae pv. tabaci Tobacco wildfire, caused by Wolf et Foster, is a common and serious bacterial disease in tobacco production worldwide. It is explosive and destructive, and can occur and cause damage throughout the entire growth period of tobacco, resulting in significant losses. Summary of the Invention
[0003] During their field investigations, the inventors discovered that the initial symptoms of tobacco wildfire disease appeared on the lower leaves. They also discovered for the first time that the pathogen could invade from the roots of the tobacco plant, be transported through the vascular bundles to the above-ground parts, and cause leaf disease (e.g., ...). Figure 1 , 2 However, current field control of tobacco wildfire disease primarily relies on foliar spraying, which is insufficient to control the pathogen at its source. Therefore, this invention changes the traditional application method, providing a root-applied treatment method for tobacco wildfire disease control, offering a theoretical basis and technical support for precise prevention and control of the disease.
[0004] To achieve the above objectives, the present invention adopts the following technical solution: A root application method for preventing tobacco wildfire disease includes the following two steps: 1) During the tobacco seedling stage, the roots are soaked in a copper hydroxide suspension; 2) On the 7th day after transplanting, apply copper hydroxide suspension for root irrigation.
[0005] Furthermore, the tobacco seedling stage mentioned in step 1) specifically refers to the hardening-off period two days before transplanting.
[0006] Furthermore, the copper hydroxide suspension used in step 1) is specifically a 500-fold dilution of 46wt% copper hydroxide water-dispersible granules.
[0007] Furthermore, the root soaking described in step 1) specifically involves uniformly applying a copper hydroxide suspension to the tobacco seedling trays until water seeps out from the bottom of the trays.
[0008] Furthermore, the copper hydroxide suspension used in step 2) is specifically a 1200-fold dilution of 46wt% copper hydroxide water-dispersible granules.
[0009] Further, the root irrigation in step 2) is specifically 150 mL of copper hydroxide suspension per plant.
[0010] Advantages of the present application: The inventor found in previous research that tobacco wild fire pathogen can invade from the root system of tobacco plants, conduct to the aboveground part of the plant through the vascular bundle and cause leaf disease. Based on this, the present application changes the traditional tobacco wild fire disease prevention and control method of leaf spraying to the root irrigation method, that is, root soaking with copper preparation before transplanting and root irrigation with copper preparation after transplanting, to form a protective layer on the root system and kill the pathogenic bacteria in the rhizosphere soil, thereby preventing the pathogenic bacteria from invading the root system and reducing the infection probability of the pathogenic bacteria from the source, improving the accuracy of the drug use and reducing the incidence of tobacco wild fire disease in the field. BRIEF DESCRIPTION OF DRAWINGS
[0011] Figure 1 A diagram of the situation of tobacco leaf disease caused by root irrigation of wild fire pathogen.
[0012] Figure 2 A diagram of the situation of tobacco wild fire pathogen invading the root system of flue-cured tobacco and transporting in the stem, wherein A is a fluorescently labeled pathogen, B is the aggregation of pathogenic bacteria at the root tip, C is the invasion of pathogenic bacteria into root hair, and D is the transportation of pathogenic bacteria in the stem. DETAILED DESCRIPTION
[0013] A root administration method for preventing and treating tobacco wild fire disease, which specifically comprises the following steps: 1) Two days before the transplanting of tobacco, 500 times of 46% copper hydroxide water dispersible granule is uniformly applied to the tobacco seedling tray, until water seeps out from the bottom of the seedling tray. 2) Seven days after the transplanting, 1200 times of 46% copper hydroxide water dispersible granule is used for root irrigation treatment, and the root irrigation amount is 150 mL per plant.
[0014] In order to make the content of the present application more convenient to understand, the technical solutions of the present application will be further described below in combination with specific embodiments, but the present application is not limited thereto.
[0015] The test methods used in the following examples are all conventional methods unless otherwise specified.
[0016] The reagents, drugs, materials and the like used in the following examples can be obtained from commercial channels unless otherwise specified.
[0017] Example 1: Effect of root administration on prevention and treatment of tobacco wild fire disease Test agent: copper hydroxide (content 46 wt%) water dispersible granule Test flue-cured tobacco variety: Yunyan 87 Test site: Zhenqian Town, Zhenghe County, Nanping City, Fujian Province Test method: Select the field where tobacco wild fire disease occurs all year round, treatment 1: 2 days before transplanting tobacco seedlings, evenly pour tobacco seedling tray with 500 times solution of 46wt% copper hydroxide water dispersible granule until water seeps out from the bottom of the tray; treatment 2: 7 days after transplanting tobacco seedlings, root irrigation treatment with 1200 times solution of 46wt% copper hydroxide water dispersible granule, irrigation amount is 150 mL / plant; treatment 3: 2 days before transplanting tobacco seedlings, evenly pour tobacco seedling tray with 500 times solution of 46wt% copper hydroxide water dispersible granule until water seeps out from the bottom of the tray, and 7 days after transplanting tobacco seedlings, root irrigation treatment with 1200 times solution of 46wt% copper hydroxide water dispersible granule, irrigation amount is 150 mL / plant; treatment 4: evenly pour tobacco seedling tray with water 2 days before transplanting, and root irrigation treatment with water 7 days after transplanting as control, irrigation amount is 150 mL / plant. The rest of the cultivation and field management measures are carried out according to the local production habits. Disease occurrence investigation was carried out at the rosette stage, vigorous growth stage and lower leaf maturation stage of tobacco, and the control effect was calculated, the results are shown in Table 1.
[0018] Table 1 Prevention and treatment effect of copper hydroxide root administration on tobacco wild fire disease
[0019] Table 1 The test results show that root soaking or root irrigation with copper hydroxide before and after transplanting has a certain control effect on tobacco wild fire disease, and the combination of the two can achieve the maximum effect.
[0020] Example 2: Comparison of the prevention and treatment effect of root administration and foliar spraying on tobacco wild fire disease Test agent: Copper hydroxide (content 46wt%) water dispersible granule Test tobacco variety: The test tobacco variety is Yunyan 87 Test site: Zhenqian Town, Zhenghe County, Nanping City, Fujian Province Test method: select the area where tobacco wild fire disease occurs all the year round, treatment 1: 2 days before transplanting tobacco seedlings, 500 times solution of 46wt% copper hydroxide water dispersible granules is uniformly sprayed on tobacco seedling tray until water seeps out from the bottom of the tray, and 7 days after transplanting, 1200 times solution of 46wt% copper hydroxide water dispersible granules is used for root irrigation treatment, the amount of root irrigation is 150mL / plant; treatment 2: 7 days after transplanting, 500 times solution of 46wt% copper hydroxide water dispersible granules is used for foliar spraying treatment, the spraying amount is 10Kg / mu, and during tobacco rosette stage (about 40 days after transplanting), 500 times solution of 46wt% copper hydroxide water dispersible granules is used for foliar spraying treatment, spraying once every 7 days, continuous spraying for 2 times, the spraying amount of each time is 25Kg / mu; treatment 3: take water immersion, root irrigation and spraying treatment as control, the amount of root irrigation, root irrigation and spraying is equivalent to that in treatment 1 and treatment 2, carry out disease occurrence investigation during the maturation period of lower leaves, calculate the control effect, the results are shown in table 2.
[0021] Table 2 Root administration and foliar spraying on tobacco wild fire disease control effect
[0022] Table 2 test results show that, using root administration and foliar spraying method, both can reduce the disease index of wild fire disease, but the control effect of root administration treatment is significantly better than that of foliar spraying.
[0023] The above only describes the preferred embodiments of the present application, and any equivalent changes and modifications made within the scope of the present application should be included in the scope of the present application.
Claims
1. A method for administering medication through the roots to prevent and control tobacco wildfire disease, characterized in that, It includes the following two steps: 1) During the tobacco seedling stage, the roots are soaked in a copper hydroxide suspension; 2) On the 7th day after transplanting, apply copper hydroxide suspension for root irrigation.
2. The method for administering medication through the roots to prevent tobacco wildfire disease according to claim 1, characterized in that, The tobacco seedling stage mentioned in step 1) specifically refers to the hardening-off period two days before transplanting.
3. The method for administering medication through the roots to prevent tobacco wildfire disease according to claim 1, characterized in that, The copper hydroxide suspension used in step 1) is specifically a 500-fold dilution of 46% copper hydroxide water-dispersible granules.
4. The method for administering medication through the roots to prevent tobacco wildfire disease according to claim 1, characterized in that, The root soaking described in step 1) specifically involves uniformly applying a copper hydroxide suspension to the tobacco seedling trays until water seeps out from the bottom of the trays.
5. The method for administering medication through the roots to prevent tobacco wildfire disease according to claim 1, characterized in that, The copper hydroxide suspension used in step 2) is specifically a 1200-fold dilution of 46% copper hydroxide water-dispersible granules.
6. The method for administering medication through the roots to prevent tobacco wildfire disease according to claim 1, characterized in that, The root irrigation mentioned in step 2) specifically involves applying 150 mL of copper hydroxide suspension to each plant.