Fermentation composition and solid beverage for promoting skin cells to moisturize and replenish water and preparation method of fermentation composition and solid beverage

Abstract

The invention relates to a fermented composition and a solid beverage for promoting skin cells to moisturize and replenish water and a preparation method of the fermented composition and the solid beverage. The tremella polysaccharide, the salmon nasal cartilage and the rice bran oil are mixed and fermented to prepare the fermentation composition capable of promoting skin cells to moisturize and moisturize, and the active ingredients of the fermentation composition are not fully exposed before being digested but are more fully exposed after being digested; therefore, the composition and the solid beverage prepared from the composition are more suitable for being transported to the skin part after being orally taken and digested by the stomach and intestine, the moisturizing of the whole skin is promoted, and the skin elasticity is enhanced.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of health food, more particularly, to a fermented composition for promoting skin cell moisturizing and a solid beverage and a preparation method thereof. BACKGROUND

[0002] The skin is mainly composed of epidermis, dermis and subcutaneous tissue, the epidermis is the outermost layer of the skin, mainly plays a barrier function; the main components of the dermis layer of the skin include collagen, elastin and hyaluronic acid, among them, collagen is the main component of the dermis, accounting for 90% of the cytoplasm of the dermis, mainly type I collagen and type III collagen.

[0003] With the growth of age and the influence of diet, environment, etc., the elastic maintenance material in the skin of the human body decreases, causing the skin to lose elasticity and lose water, causing many skin problems, maintaining the moisture of cells can reduce the occurrence of problem skin and delay aging. Therefore, it is extremely important to moisturize and keep the skin elastic.

[0004] At present, there are various moisturizing and moisturizing skin care products on the market, but these skin care products are mainly used for local skin care, such as being used as cosmetics on facial skin. When used on the whole body, the required amount is large, the cost is high, and the use process is cumbersome, which is not practical in daily life.

[0005] The applicant submitted a patent application with application number 202410061878X and the invention name of moisturizing and moisturizing composition, solid beverage and preparation method in 2024, which is used for moisturizing and moisturizing the skin of the whole body. However, the above product still has some problems, for example, the active ingredients are completely exposed, which affects the oral effect.

[0006] Therefore, a new moisturizing and moisturizing solid beverage is needed. SUMMARY

[0007] To solve the above problems, the present application provides a method for preparing a fermented composition for promoting skin cell moisturizing and moisturizing, comprising the step of mixing tremella polysaccharide, salmon nasal cartilage powder and rice bran oil for fermentation.

[0008] In one specific embodiment, during the fermentation process, Kluyveromyces marxianus and Lactobacillus plantarum are used for fermentation.

[0009] In one specific embodiment, the method comprises the following steps: S1: preparing a tremella polysaccharide extract solution; S2: preparing a fermentation substrate with the tremella polysaccharide extract solution, salmon nasal cartilage powder and rice bran oil, and inoculating fermentation microorganisms for fermentation to obtain a fermentation product; S3: spray-drying the fermentation product to obtain the fermentation composition.

[0010] In one embodiment, S2 comprises the following steps: S21: adding 5% glucose and 1% peptone to the tremella polysaccharide extract, and adjusting the pH to 4.5 to prepare a fermentation raw material; S22: inoculating the fermentation raw material with K. marxianus and L. plantarum (10 6 CFU / ml each) and aerobically fermenting at 30°C for 24 h to obtain a primary fermentation product; S23: adding 1% chitosan oligosaccharide, 5% rice bran oil powder, 1% hyaluronic acid sodium, and 2% salmon nasal cartilage powder to the primary fermentation product, and continuing to aerobically ferment at 30°C for 36 h to obtain the fermentation product.

[0011] In one embodiment, in S21, fresh grape skin homogenate is also added to the fermentation raw material, and the weight ratio of the fresh grape skin homogenate to the tremella polysaccharide extract is 1:4.

[0012] The present application also provides a fermentation composition for promoting skin cell moisturizing and hydration prepared by the above method.

[0013] The present application also provides a solid beverage for promoting skin moisturizing and hydration comprising the above fermentation composition.

[0014] In one embodiment, the solid beverage comprises the following components by weight: 30 parts of the fermentation composition, 15 parts of fructooligosaccharide, parts of isomaltooligosaccharide, 15 parts of isomalt, 1 part of vitamin C, 0.02 parts of nicotinamide, and 1 part of gossypium hirsutum L. powder.

[0015] The present application prepares a fermentation composition for promoting skin cell moisturizing and hydration by mixed fermentation of tremella polysaccharide, salmon nasal cartilage, and rice bran oil. The active ingredients of the fermentation composition are not sufficiently exposed before digestion, but are more fully exposed after digestion, which makes the composition and the solid beverage prepared therefrom more suitable for being transported to the skin site after oral administration and gastrointestinal digestion, promoting skin moisturizing and hydration and enhancing skin elasticity. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 Effects of the compositions of Examples and Comparative Examples on the expression of HAS1 in HaCat cells after simulated digestion and without digestion.

[0017] Figure 2 Effects of the compositions of Examples and Comparative Examples on the water loss inhibition rate of zebrafish embryos after simulated digestion and without digestion.

[0018] Figure 3 Effects of the solid beverages of the examples and comparative examples on the skin water content of the takers.

[0019] Figure 4 Effects of the solid beverages of the examples and comparative examples on the skin elasticity of the takers. DETAILED DESCRIPTION

[0020] The principles and features of the present application are described below in conjunction with the accompanying drawings, in which the examples are used to explain the present application and are not intended to limit the scope of the present application.

[0021] 1. Preparation example of tremella polysaccharide-salmon nasal cartilage-rice bran oil fermentation composition 1) Extraction of tremella polysaccharide Dry tremella was crushed and mixed with water at a weight ratio of 1:100, and reacted at 100°C under 2 atm for 3 hours. After cooling to room temperature, the filtrate was collected and concentrated by rotary evaporation to 1 / 5 of the original volume to obtain a tremella polysaccharide extract. 2) Primary fermentation Fresh grape skins were homogenized and mixed with the tremella polysaccharide extract at a weight ratio of 1:4. 5% glucose and 1% proteose peptone were added, and 10 mM citrate buffer was added to adjust the pH to 4.5 to prepare the fermentation raw material.

[0022] Kluyveromyces marxianus and Lactobacillus plantarum (10 6 CFU / ml each) were inoculated into the fermentation raw material, and aerobic fermentation was carried out at 30°C for 24 h to obtain a primary fermentation product.

[0023] 3) Secondary fermentation 1% chitosan, 5% rice bran oil powder, 1% sodium hyaluronate, and 2% salmon nasal cartilage powder were added to the primary fermentation product, and aerobic fermentation was continued at 30°C for 36 h to obtain a secondary fermentation product.

[0024] 4) Spray drying: The secondary fermentation product was spray dried to obtain a tremella polysaccharide-salmon nasal cartilage-rice bran oil fermentation composition.

[0025] In the above preparation process, the percentage of each component is the weight percentage of the component to the tremella polysaccharide extract.

[0026] 2. Comparative Example 1 The same amount of commercially available tremella polysaccharide extract, chitosan, rice bran oil powder, sodium hyaluronate, and salmon nasal cartilage powder as in the example were mixed to obtain Comparative Example 1.

[0027] 3. Comparative Example 2 The other steps were the same as in the example, except that fresh grape skins were not added during primary fermentation.

[0028] 4. Comparative Example 3 The other steps were the same as in the example, except that the components were mixed together and fermented at 30°C for 60 h.

[0029] 5. Fermentation composition increases HAS1 expression in fibroblast cells The fermentation composition was dissolved in water at a concentration of 1%, pepsin was added, and the mixture was treated at 37°C for 40 min to simulate digestion conditions. After digestion, the pepsin was inactivated by heating to 90°C, and then the mixture was cooled to room temperature to obtain a digestion product simulating gastric acid decomposition.

[0030] The test was performed using human immortalized keratinocytes (HaCaT). After the cells were cultured and trypsinized at a concentration of 0.25%, the cells were counted, and then seeded in a 24-well plate (60,000 cells / well, 500 μL / well). After 24 h of culture in DMEM medium containing 10% FBS, the medium was replaced with DMEM medium containing 2% FBS to perform starvation culture for 24 h. After washing with DMEM medium containing 2% FBS three times, 500 μL of the fermentation composition of the example or the composition of Comparative Examples 1 to 3, with or without digestion, was added to the culture, and incubated for 6 h. Then, the cells were collected, and the expression of hyaluronic acid synthase 1 (HAS1) was detected by RT-PCR. The expression of the sample incubated in DMEM medium containing 2% FBS for 6 h was normalized.

[0031] The results are shown in Table 1. Figure 1 As shown in Table 1, the fermentation composition of the example and the fermentation composition of Comparative Examples 2 and 3 did not significantly increase the expression of HAS1 without digestion, and were not as good as Comparative Example 1. However, all of the three fermentation compositions promoted the expression of HAS1 after digestion, and the fermentation composition of the example increased the expression of HAS1 by more than 35 times, allowing the cells to synthesize hyaluronic acid. This indicates that the fermentation composition of the example is suitable for use as a food product, rather than as a cosmetic product for external use.

[0032] 6. Preparation of solid beverage The solid beverage was prepared by mixing 30 parts of the fermentation composition, 15 parts of fructooligosaccharide, 15 parts of isomaltooligosaccharide, 15 parts of isomalt, 1 part of vitamin C, 0.02 parts of nicotinamide, and 1 part of Gossypium arboreum L. powder, and then sieving the mixture through a 20-mesh sieve.

[0033] 7. Solid beverage increases the dehydration inhibition rate of zebrafish embryos The model of inducing dehydration by increasing the osmotic pressure of fish embryo culture solution was used for testing. 24 three-day zebrafish embryos were exposed to 15 g / L NaCl and 0.71 g / L solid beverage aqueous solution as the test group, while a model control group (15 g / L NaCl) and a blank control group (fish embryo culture solution) were set up. After 3 h of exposure, the fish embryos were photographed under a microscope to test the tail area and statistically analyzed, and the average value of each group was taken.

[0034] The formula for calculating the dehydration inhibition rate of zebrafish embryos is as follows: In the formula, S is the average value of the tail area of zebrafish embryos in the test group; C is the average value of the tail area of zebrafish embryos in the blank control group; M is the average value of the tail area of zebrafish embryos in the model control group.

[0035] The results are shown in Table 1. Figure 2 As shown in Table 1, the dehydration inhibition rate of zebrafish embryos caused by the solid beverage of the embodiment of the present application without digestion is not significantly different from that of each comparative example, but the dehydration inhibition rate caused by the solid beverage after digestion is significantly higher than that of each group, reaching more than 40%. It can be seen that although the undigested embodiment and comparative example can also improve the dehydration inhibition rate, digestion can significantly improve the rehydration effect of the solid beverage of the embodiment. It can be seen that the digestion of the human gastrointestinal tract is also very important for the solid beverage of the present application, which may be because after digestion, the relevant active ingredients can be exposed to stimulate cell secretion of HAS1, thereby improving the synthesis of hyaluronic acid and improving the rehydration effect.

[0036] 8. Human effect test of solid beverage The test subjects were 30-50-year-old women with simple skin dryness and relaxation, and were randomly divided into 12 groups according to the age of the test subjects, with 50 effective people in each group. Among them, 12 groups of people ate the basic diet + 1 package of solid beverage (one of the solid beverages of embodiments 1-6 and the solid beverages of comparative examples 1-6) every day, and the skin moisture content and elasticity of the face were tested before and after 30 days of eating using a skin tester, and the average value of each group was taken.

[0037] The skin moisture content was tested using a skin keratin layer moisture tester Cornemeter CM825 (Courage & Khazaka, Germany). The skin moisture content before use was recorded as W0, and the skin moisture content after 30 days of use was recorded as W. The calculation method of the increase in skin moisture content was as follows: Skin elasticity R2 value is measured by Cutometer (Courage & Khazaka, Germany); the higher the value (closer to 1), the better the skin elasticity; the skin elasticity R2 value before use is recorded as T0, and the skin elasticity R2 value after 30 days of use is recorded as T; the calculation method of the increase of skin elasticity is: The results are shown in Figure 3 and 4 The increase of skin moisture content and the increase of skin elasticity by using the solid beverage of the present application are significantly higher than those of other groups.

[0038] In summary, the fermented composition of the present application adds salmon nasal cartilage and rice bran oil, etc., to provide proteoglycans, ceramides, and hyaluronic acid to achieve the effect of moisturizing and hydrating, and the tremella polysaccharide can inhibit the generation of free radicals. In addition, through a special fermentation process, the fermented composition contains substances that can promote the synthesis and secretion of hyaluronic acid by skin cells, greatly improving the expression of hyaluronic acid synthase and the synthesis of hyaluronic acid, thereby achieving better moisturizing and hydrating effects.

[0039] The above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method for preparing a fermented composition that promotes skin cell moisturizing and hydration, characterized in that, This includes the step of mixing and fermenting tremella polysaccharide, salmon nasal cartilage powder, and rice bran oil.

2. The method according to claim 1, characterized in that, The fermentation process uses Kluyveromyces martensii and Lactobacillus plantarum.

3. The method according to claim 2, characterized in that, Includes the following steps: S1: Preparation of Tremella fuciformis polysaccharide extract; S2: Prepare a fermentation substrate using the above-mentioned Tremella polysaccharide extract, salmon nasal cartilage powder, and rice bran oil, and inoculate with fermentation microorganisms to carry out fermentation to obtain fermentation products; S3: Spray dry the fermentation product to obtain the fermentation composition.

4. The method according to claim 3, characterized in that, S2 includes the following steps: S21: Add 5% glucose and 1% peptone to the above-mentioned Tremella polysaccharide extract, and adjust the pH to 4.5 to prepare fermentation raw material; S22: Inoculate the fermentation feed with Kluyveromyces martensii and Lactobacillus plantarum (10 μL each). 6 (CFU / ml), aerobic fermentation at 30℃ for 24 h yielded the primary fermentation product; S23: Add 1% chitosan oligosaccharide, 5% rice bran oil powder, 1% sodium hyaluronate and 2% salmon nasal cartilage powder to the primary fermentation product, and continue aerobic fermentation at 30°C for 36 h to obtain the fermentation product.

5. The method according to claim 3, characterized in that, In S21, fresh grape skin homogenate was also added to the fermentation raw materials, and the weight ratio of the fresh grape skin homogenate to the tremella polysaccharide extract was 1:

4.

6. A fermented composition that promotes skin cell hydration and moisturization, characterized in that, It is prepared by the method according to any one of claims 1-5.

7. A solid beverage that promotes skin hydration and moisturization, characterized in that, Includes the fermentation composition of claim 6.

8. The solid beverage according to claim 7, characterized in that, The product comprises the following components by weight: 30 parts fermentation composition, 15 parts fructooligosaccharide, 1 part isomaltooligosaccharide, 15 parts isomaltitol, 1 part vitamin C, 0.02 parts nicotinamide, and 1 part Kanzan cherry blossom powder.

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