Extraction method and application method of effective components of purslane capable of reducing uric acid

Abstract

The invention discloses an extraction method and application of effective components of purslane for reducing uric acid, and relates to the technical field of homology of medicine and food. Edible and medicinal substance portulaca oleracea is used as a raw material to prepare the extract, effective components rich in the portulaca oleracea are fully extracted according to the dissolvability difference of the effective components in a water phase and an alcohol phase, although the water extract is poor in stability and the alcohol extract is high in irritation, the stability is remarkably improved and the irritation is reduced through molecular interaction after the water extract and the alcohol extract are mixed; the raw materials are mixed to serve as a single prescription or are combined with other Chinese herbal medicines to serve as a compound to be applied to food products, and the food products can be used for daily eating, health preserving and conditioning of common people.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of food and medicine homology, in particular to an extraction method of effective components of Portulaca oleracea for reducing uric acid and an application method thereof. BACKGROUND

[0002] Portulaca oleracea L. is an annual succulent herbaceous plant of Portulacaceae Portulaca. Portulaca oleracea has fast reproduction, high yield, strong environmental adaptability and rich resources, and is one of important food and medicine herbs. Portulaca oleracea tastes sour and cold, and belongs to liver and large intestine channels. The whole plant is used for medicine and has the effects of clearing heat and reducing dampness, detoxifying and detumescence, anti-inflammatory, thirst-relieving and diuretic. Portulaca oleracea is rich in alkaloids, terpenes, polysaccharides, coumarins, flavonoids, organic acids and other chemical components; modern research shows that Portulaca oleracea has the effects of anti-inflammatory, antioxidant, analgesic, antibacterial, hypolipidemic, hypoglycemic, antitumor and immune enhancement, and is widely used in the fields of medicine, health care products and skin care products.

[0003] Hyperuricemia (HUA) refers to that the fasting blood uric acid level of adults is more than 420 μmol / L without male and female distinction under normal purine diet. Gout is directly related to hyperuricemia caused by purine metabolism disorder and / or reduced uric acid excretion. Chinese herbal medicine has a long history in the prevention and treatment of gout, and has the characteristics and advantages of multi-target, wide source, small side effect, high safety, etc.; it plays a therapeutic role through reducing uric acid, anti-inflammatory, antioxidant, kidney protection, etc., and has obvious curative effect and low adverse reaction rate. SUMMARY

[0004] The purpose of the present application is to provide an extraction method of effective components of Portulaca oleracea for reducing uric acid and an application method thereof. The extraction is prepared by taking food and medicine homology material Portulaca oleracea as raw material, and the effective components richly contained therein are fully extracted according to the solubility difference of the effective components in water phase and alcohol phase. Although the water extract has poor stability and the alcohol extract has large irritation, the stability is significantly improved and the irritation is reduced through molecular interaction after mixing, and the mixture can be used as a single prescription or combined with other Chinese herbal medicines as a compound prescription in food products, which can be used for daily consumption and health conditioning of ordinary people.

[0005] In order to achieve the above purpose, the present application discloses an extraction method of effective components of Portulaca oleracea for reducing uric acid, which comprises the following steps:

[0006] S1, collecting the dried above-ground part of Portulaca oleracea, selecting Portulaca oleracea raw material, removing impurities, and cleaning and drying with deionized water;

[0007] S2, crushing the above-mentioned Portulaca oleracea raw material to 50-200 meshes, and dividing the Portulaca oleracea powder into two equal parts;

[0008] S3, half of the pigweed powder is mixed evenly, and alcohol extraction is performed to obtain an alcohol extraction liquid;

[0009] S4, the other half of the pigweed powder is extracted by water extraction to obtain a water extraction liquid;

[0010] S5, the alcohol extraction liquid and the water extraction liquid are mixed at a volume ratio of 1: (1-10) to prepare a pigweed effective component extraction liquid.

[0011] Preferably, the alcohol extraction method in step S3 comprises the following steps:

[0012] S3-1, the pigweed powder is placed in a low-alcohol solution, the liquid surface is ensured to immerse the pigweed powder, and the extraction is performed in a closed container in a cool place for 30 minutes to 6 months;

[0013] S3-2, after the extraction is completed, the residue is filtered to obtain the alcohol extraction liquid;

[0014] S3-3, the used ethanol is edible grade;

[0015] S3-4, the alcohol extraction liquid is sterilized and filled.

[0016] Preferably, in step S3-1, the low-alcohol is ethanol, and the volume fraction of the low-alcohol in the low-alcohol solution is 20% to 70%.

[0017] Preferably, the water extraction method in step S4 comprises the following steps:

[0018] S4-1, the pigweed powder is added to pure water and mixed thoroughly, and the liquid surface is ensured to immerse, and heated reflux extraction is performed for 30 minutes to 3 hours;

[0019] S4-2, after the extraction is completed, the residue is filtered to obtain the water extraction liquid;

[0020] S4-3, sterilization and filling are performed.

[0021] Preferably, in step S4-1, the extraction liquid can be evaporated by rotation, freeze-dried, etc. to be paste or powder.

[0022] The application also provides an application of the above-mentioned pigweed effective component extraction method for reducing uric acid, and the pigweed effective component extraction liquid prepared by the above-mentioned extraction method has the effect of reducing blood uric acid of hyperuricemia mice, and can be used in food products which are liquid, solid or semi-solid.

[0023] Preferably, the pigweed effective component extraction liquid is used alone or after being matched with other Chinese herbal medicines, has the effect of reducing blood uric acid of hyperuricemia mice.

[0024] Preferably, the Chinese herbal medicine matched with the extract of effective components of Portulaca oleracea L. includes any one or more of the following: Taraxacum mongolicum, honeysuckle, coix seed, licorice, dendrobium officinale, Chinese yam, and medlar, and the matching mode is co-extraction or separate extraction of Portulaca oleracea L. powder and Chinese herbal medicine powder.

[0025] Therefore, the application has the following beneficial effects by using the above-mentioned extraction method of effective components of Portulaca oleracea L. for reducing uric acid and the application method thereof:

[0026] 1. The main active components rich in Portulaca oleracea L. include polysaccharides, alkaloids, flavonoids, organic acids, etc., which are the material basis of the biological activity of Portulaca oleracea L. The extraction solvents of water extract and alcohol extract are different in polarity, and the corresponding product compositions are also different, such as the difference in the composition of polysaccharides and flavonoids. Through the organic combination of the two, the expression of effective components can be improved, the fat-soluble and water-soluble uric acid-reducing active substances can be enriched, and through the synergistic effect of multiple pathways, the product obtained by a single extraction pathway is more stable and more comprehensive in reducing uric acid.

[0027] 2. In the present application, various matching raw materials are screened based on Portulaca oleracea L., wherein Portulaca oleracea L., Taraxacum mongolicum belong to heat-clearing and detoxifying type, honeysuckle and coix seed belong to heat-clearing and dampness-removing type, licorice and Chinese yam are qi-tonifying type, dendrobium officinale and medlar are yin-tonifying type, and through the matching of various raw materials, the purpose of mutual regulation and synergistic effect is achieved.

[0028] The technical solutions of the present application will be further described in detail below with reference to the accompanying drawings and examples. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 Figure for the effect of test samples on the blood uric acid level of mice. DETAILED DESCRIPTION

[0030] Unless otherwise defined, the technical terms or scientific terms used in the present application shall have the usual meaning understood by a person with ordinary skill in the art to which the present application belongs.

[0031] In addition, it should be understood that although the present specification is described in terms of embodiments, each embodiment does not contain only one independent technical solution, and the description manner of the specification is only for the sake of clarity, and the person skilled in the art should consider the specification as a whole, and the technical solutions in each example can also be appropriately combined to form other embodiments which can be understood by the person skilled in the art. These other embodiments are also covered by the protection scope of the present application.

[0032] The present application will be further described below in combination with examples and drawings, and the various chemicals and reagents used in the examples are commercially available.

[0033] Example 1

[0034] The embodiment provides a water extraction method of Portulaca oleracea L. effective components, and comprises the following steps:

[0035] (1) collecting dry above-ground parts of Portulaca oleracea L., detecting and selecting, and drying after washing with deionized water.

[0036] (2) crushing the raw material to 50 meshes.

[0037] (3) taking 50 parts of Portulaca oleracea L. powder and adding 100 parts of purified water to mix fully, ensuring that the liquid surface is immersed, and heating to reflux extraction at 100 DEG C for 30 minutes.

[0038] (4) after the extraction is completed, filtering the residue, and collecting water extraction extract.

[0039] (5) cooling and filtering, collecting water extraction extract, ensuring that the storage container is well sealed and lightproof, and storing in a cool place.

[0040] Example 2

[0041] The embodiment provides an alcohol extraction method of Portulaca oleracea L. effective components, and comprises the following steps:

[0042] (1) collecting dry above-ground parts of Portulaca oleracea L., detecting and selecting, and drying after washing with deionized water.

[0043] (2) crushing the raw material to 50 meshes.

[0044] (3) taking 50 parts of Portulaca oleracea L. powder and adding 100 parts of 20% ethanol solution to mix fully, ensuring that the liquid surface is immersed, and placing in a sealed container to extract in a cool place for 1 month.

[0045] (4) after the extraction is completed, filtering the residue, and collecting alcohol extraction extract.

[0046] (5) sterilizing and filling, ensuring that the storage container is well sealed and lightproof, and storing in a cool place.

[0047] Example 3

[0048] The embodiment discloses an extraction method of Portulaca oleracea L. effective components, mixing water extraction extract prepared in the example 1 with alcohol extraction extract prepared in the example 2 in a volume ratio of 8:1 to prepare Portulaca oleracea L. effective component extract.

[0049] Example 4

[0050] The embodiment discloses an extraction method of effective components of Portulaca oleracea L. The water extraction liquid prepared in the embodiment 1 and the alcohol extraction liquid prepared in the embodiment 2 are mixed in a volume ratio of 5:1 to prepare the effective component extraction liquid of Portulaca oleracea L.

[0051] Embodiment 5

[0052] The embodiment discloses an extraction method of effective components of Portulaca oleracea L. The water extraction liquid prepared in the embodiment 1 and the alcohol extraction liquid prepared in the embodiment 2 are mixed in a volume ratio of 7:3 to prepare the effective component extraction liquid of Portulaca oleracea L.

[0053] Embodiment 6

[0054] The embodiment discloses an extraction method of effective components of Portulaca oleracea L. The water extraction liquid prepared in the embodiment 1 and the alcohol extraction liquid prepared in the embodiment 2 are mixed in a volume ratio of 1.5:1 to prepare the effective component extraction liquid of Portulaca oleracea L.

[0055] Application example

[0056] The effective component extraction liquid of Portulaca oleracea L. prepared in the above embodiments 1-6 is detected on male SPF level mice, which are randomly divided into 5 groups, 10 in each group. The test group design includes a blank group, a model group, a positive drug group, a low-dose Portulaca oleracea L. group and a high-dose Portulaca oleracea L. group, specifically: (1) the blank group: the normal feeding mice; (2) the model group: the uricogenic drug (potassium oxonate and hypoxanthine) is poured into the stomach; (3) test group 1: the uricogenic drug and the low-dose Portulaca oleracea L. extract (0.5g / kg / d) are poured into the stomach; (4) test group 2: the uricogenic drug and the high-dose Portulaca oleracea L. extract (1.5g / kg / d) are poured into the stomach. After blood sampling, the serum uric acid level is detected. The results are shown in the table as follows: Figure 1 The serum uric acid (UA) level of the model group compared with the blank group confirms that the model agent (potassium oxonate + hypoxanthine) successfully establishes a hyperuricemia model. The experimental results of the embodiments 1-6 show that the low-dose group and the high-dose group can reduce the UA level of the model animals, but the uric acid reduction effect of the high-dose group is always better than that of the low-dose group, which embodies a clear dose-effect relationship. When the alcohol extract and the water extract are compounded in different proportions, the UA reduction level shows a trend of first increasing and then weakening, which indicates that there is an optimal compatibility window for the synergistic effect of the two, and the proportion mismatch will weaken the overall efficacy. Under the best proportion, a more stable and more comprehensive uric acid reduction effect is realized than the single extract.

[0057] Embodiment 7

[0058] The effective component extract of purslane in Example 5 is mixed with the water extract of honeysuckle, the water extract of dandelion, and the water extract of Chinese wolfberry at a volume ratio of 3:2:1 to prepare a food product with the function of reducing blood uric acid of hyperuricemia. The method of mixing the water extracts of the above Chinese herbal medicines is the same as the water extraction method provided in Example 1, except that the raw materials are replaced by the corresponding Chinese herbal medicines.

[0059] Example 8

[0060] The effective component extract of purslane in Example 5 is mixed with the alcohol extract of honeysuckle, the alcohol extract of licorice, and the alcohol extract of Chinese wolfberry at a volume ratio of 1:1:1:1 to prepare a food product with the function of reducing blood uric acid of hyperuricemia. The method of mixing the alcohol extracts of the above Chinese herbal medicines is the same as the water extraction method provided in Example 2, except that the raw materials are replaced by the corresponding Chinese herbal medicines.

[0061] Example 9

[0062] The purslane extract, the dandelion extract, the yiyi extract, the Dendrobium officinale extract, and the yam extract are mixed at a volume ratio of 1:1:1:1:1 to prepare a food product with the function of reducing blood uric acid of hyperuricemia. In this embodiment, the purslane extract is prepared as in Example 5, and the dandelion extract, the yiyi extract, the Dendrobium officinale extract, and the yam extract are extracted by any one of the extraction methods in Example 1 or Example 2, except that the raw materials are replaced by the corresponding Chinese herbal medicines.

[0063] Therefore, the present application provides an extraction method of purslane effective components for reducing uric acid and an application method thereof. The purslane, which is a food and medicine homologous substance, is used as a raw material to prepare an extract. According to the solubility difference of the effective components in water and alcohol, the effective components are fully extracted. Although the water extract has poor stability and the alcohol extract has great irritation, the stability is significantly improved and the irritation is reduced after mixing through molecular interaction. The mixture can be used as a single prescription or a compound prescription after being mixed with other Chinese herbal medicines and applied in food products. The mixture can be used for daily consumption and health conditioning of ordinary people.

[0064] The above specific embodiments further illustrate the purpose, technical solutions, and beneficial effects of the present application. It should be understood that the above description is only a specific embodiment of the present application and does not limit the protection scope of the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A method for extracting the effective components of purslane that lower uric acid, characterized in that, Includes the following steps: After removing impurities, washing, and drying, the purslane raw material is pulverized to 50-200 mesh; its alcohol extract and water extract are extracted separately, and the alcohol extract and water extract of purslane are mixed at a volume ratio of 1:(1-10) to prepare the purslane effective component extract.

2. The method for extracting the effective components of purslane for lowering uric acid according to claim 1, characterized in that, The alcohol extract of purslane was obtained by the following steps: (1) Place the purslane powder in a low alcohol solution, ensuring that the liquid surface is submerged in the purslane powder; and place it in a sealed container in a cool place for full extraction, with an extraction time of 30 minutes to 6 months; (2) After extraction, filter the residue and collect the alcohol extract of purslane; (3) The alcohol extract is sterilized and filled.

3. The method for extracting the effective components of purslane for lowering uric acid according to claim 1, characterized in that, The water extract of purslane is obtained through the following steps: (1) Add purslane powder to purified water and mix thoroughly to ensure that the liquid surface is submerged. Heat and reflux at 95-105℃ for 30 minutes to 3 hours to extract. (2) After extraction, filter the residue and collect the water extract of purslane; (3) Sterilize and fill the product.

4. The method for extracting the effective components of purslane for lowering uric acid according to claim 2, characterized in that, The lower alcohol used in the alcohol extraction method is ethanol, and the volume fraction of the lower alcohol in the lower alcohol solution is 20%-70%.

5. The method for extracting the effective components of purslane for lowering uric acid according to claim 3, characterized in that, In the water extraction method, the extract is rotary evaporated or freeze-dried to a paste or powder form.

6. The application of a method for extracting the uric acid-lowering active ingredients from purslane as described in claims 1-5, characterized in that, The purslane extract prepared by the above extraction method is used in the preparation of food products that can reduce blood uric acid in patients with hyperuricemia; the food products are liquid, solid or semi-solid.

7. The application of the method for extracting the effective components of purslane for lowering uric acid according to claim 6, characterized in that, The purslane extract, either alone or in combination with other traditional Chinese medicines, is used as a compound in food preparation.

8. The application of the method for extracting the effective components of purslane for lowering uric acid according to claim 7, characterized in that, The Chinese herbal medicines that are used in combination with the purslane active ingredient extract are food and medicine homologous substances, including any one or more of dandelion, honeysuckle, coix seed, licorice, dendrobium officinale, yam and wolfberry. The combination method is to co-extract and combine purslane powder with Chinese herbal medicine powder or to extract and combine them separately.

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