Composition for inhibiting propionibacterium acnes, preparation method thereof and acne-removing cosmetic

Abstract

The invention relates to the field of cosmetics, in particular to a composition for inhibiting propionibacterium acnes, a preparation method of the composition and an acne removing cosmetic. The composition for inhibiting the propionibacterium acnes is prepared from the following components: a manglietia extract solution and a pionin solution, the mass ratio of the manglietia fordiana extract solution to the Paxonine solution is (1-4): (4-1). According to the technical scheme, the composition containing the manglietia fordiana extract solution and the Paionin solution is provided, and the composition can stably inhibit growth of propionibacterium acnes, so that the effect of removing acnes is achieved. The two components in the composition are in the ratio range, the synergistic interaction effect can be achieved, and the effect of inhibiting propionibacterium acnes is improved. The two components in the composition are green and environmentally friendly, and compared with chemical acne removing components such as salicylic acid, azelaic acid, benzoyl peroxide and tretinoin, the acne removing effect is stable, and the composition is green, natural and free of irritation and side effects.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular, to a composition for inhibiting propionibacterium acnes and a preparation method thereof, and an acne-removing cosmetic. BACKGROUND

[0002] With the acceleration of the pace of life and the increase of social pressure, acne problems have become an important factor affecting the skin health of a wide range of people.

[0003] At present, the acne-removing products on the market mostly use chemical components such as salicylic acid, azelaic acid, benzoyl peroxide and retinoids for acne removal, but these acne-removing components are unstable in effect and have great irritation and are prone to side effects. SUMMARY

[0004] The purpose of the embodiments of the present application is to provide a composition for inhibiting propionibacterium acnes and a preparation method thereof, and an acne-removing cosmetic.

[0005] In a first aspect, the present application provides a composition for inhibiting propionibacterium acnes, which is composed of the following components: a solution of manglietia bark extract and a solution of paeonia suffruticosa andrews. The mass ratio of the solution of manglietia bark extract and the solution of paeonia suffruticosa andrews is (1-4):(4-1).

[0006] The above technical solution provides a composition containing a solution of manglietia bark extract and a solution of paeonia suffruticosa andrews, which can stably inhibit the growth of propionibacterium acnes, thereby achieving the effect of acne removal. The two components in the composition can produce a synergistic effect within the above-mentioned ratio range, thereby improving the effect of inhibiting propionibacterium acnes. Moreover, the two components in the composition are green and environmentally friendly, and the acne-removing effect is stable and green, natural, non-irritating and without side effects, as compared with chemical acne-removing components such as salicylic acid, azelaic acid, benzoyl peroxide and retinoids.

[0007] In other embodiments of the present application, the mass ratio of the solution of manglietia bark extract and the solution of paeonia suffruticosa andrews is 1:1.

[0008] In other embodiments of the present application, the concentration of the solution of manglietia bark extract is 1%-3% by mass percentage.

[0009] In other embodiments of the present application, the mass ratio of the solution of manglietia bark extract and the solution of paeonia suffruticosa andrews is 1:1.

[0010] In other embodiments of the present application, the concentration of the solution of manglietia bark extract is 1%-3% by mass percentage.

[0011] In other embodiments of the present application, the concentration of the solution of paeonia suffruticosa andrews is 0.5%-1% by mass percentage.

[0012] In other embodiments of the present application, the mangrove extract solution comprises: an alcohol extract of the mangrove plant.

[0013] In other embodiments of the present application, the mangrove extract solution comprises: an alcohol extract of the mangrove plant.

[0014] In other embodiments of the present application, the mass ratio of the mangrove extract solution and the piroctone olamine solution is (1-3):(3-1).

[0015] In a second aspect, the present application provides a preparation method of a composition for inhibiting Propionibacterium acnes, comprising: extracting a mangrove plant to obtain a mangrove extract, and diluting the mangrove extract to obtain a mangrove extract solution; mixing piroctone olamine with water to obtain a piroctone olamine solution; mixing the mangrove extract solution and the piroctone olamine solution according to a mass ratio of (1-4):(4-1).

[0016] In other embodiments of the present application, the diluting of the mangrove extract to obtain the mangrove extract solution comprises diluting the mangrove extract to a concentration of 1%-3%.

[0017] In other embodiments of the present application, the mixing of the piroctone olamine with water to obtain the piroctone olamine solution comprises diluting the piroctone olamine to a concentration of 0.5%-1%.

[0018] In a third aspect, the present application provides an acne-removing cosmetic, comprising the composition for inhibiting Propionibacterium acnes according to any one of the first aspect, or the acne-removing cosmetic comprises the composition for inhibiting Propionibacterium acnes prepared according to any one of the second aspect. DETAILED DESCRIPTION

[0019] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments of the present application.

[0020] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of the present application.

[0021] It is found through research that acne, especially acne, is mainly caused by overgrowth of Propionibacterium acnes; therefore, by inhibiting the growth of Propionibacterium acnes, the acne-removing effect is achieved.

[0022] Based on this, the application provides a composition for inhibiting propionibacterium acnes, which is composed of: a solution of mangrove extract and a solution of piroctone olamine; The mass ratio of the solution of mangrove extract and the solution of piroctone olamine is (1-4):(4-1).

[0023] The above technical solution provides a composition containing a solution of mangrove extract and a solution of piroctone olamine (quaternary ammonium salt-73) extract. The composition can stably inhibit the growth of propionibacterium acnes, thereby achieving the effect of removing acne. The two components in the composition can produce a synergistic effect within the above-mentioned ratio range, thereby improving the effect of inhibiting propionibacterium acnes. Moreover, the two components in the composition are green and environmentally friendly. Compared with chemical acne-removing components such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoids, the composition has stable acne-removing effect and is green, natural, and non-irritating, without side effects.

[0024] Further optionally, for example, in some embodiments of the application, the mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, or a range between any two of the foregoing values.

[0025] Further optionally, in some embodiments of the application, the mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 1:1.

[0026] Further, in some embodiments of the application, the concentration of the solution of mangrove extract is 1%-3% by mass percentage.

[0027] For example, in some embodiments of the application, the concentration of the solution of mangrove extract is 1%, 1.5%, 1.8%, 2%, 2.1%, 2.5%, 2.8%, 3%, or a range between any two of the foregoing values, by mass percentage.

[0028] Further, in some embodiments of the application, the concentration of the solution of piroctone olamine is 0.5%-1% by mass percentage.

[0029] For example, in some embodiments of the application, the concentration of the solution of piroctone olamine is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or a range between any two of the foregoing values, by mass percentage.

[0030] Further, in some embodiments of the application, the solution of mangrove extract comprises an alcohol extract of mangrove plants.

[0031] Further, in some embodiments of the present application, the mangrove extract includes: an ethanol extract of the mangrove plant.

[0032] Further, in some embodiments of the present application, the mass ratio of the mangrove extract solution and the piroctone olamine solution is (1-3):(3-1).

[0033] Some embodiments of the present application provide a method for preparing a composition for inhibiting Propionibacterium acnes, comprising: The mangrove plant is extracted by alcohol to obtain an extract, and the extract is diluted to obtain a mangrove extract solution. The piroctone olamine is mixed with water to obtain a piroctone olamine solution. The mangrove extract solution and the piroctone olamine solution are mixed uniformly at a mass ratio of (1-4):(4-1).

[0034] In the above technical solution, the composition containing the mangrove extract solution and the piroctone olamine solution can be effectively prepared, which can stably inhibit the growth of Propionibacterium acnes, thereby achieving the effect of removing acne.

[0035] Further optionally, in some embodiments of the present application, the mangrove plant is extracted by alcohol to obtain an extract, comprising: The mangrove plant is extracted by alcohol to obtain an extract.

[0036] Further optionally, in some embodiments of the present application, after the mangrove plant is extracted by alcohol, the effective components are extracted through steps such as concentration and extraction.

[0037] Further optionally, in some embodiments of the present application, the piroctone olamine (quaternary ammonium salt-73) is an artificially synthesized chemical substance, which is obtained by pure chemical synthesis and can be synthesized by conventional methods in the art.

[0038] Further optionally, in some embodiments of the present application, the piroctone olamine (quaternary ammonium salt-73) can be obtained by market purchase.

[0039] Further optionally, for example, in some embodiments of the present application, the mangrove extract solution and the piroctone olamine solution are mixed uniformly at a mass ratio of 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, or a range between any two of the foregoing values.

[0040] Further, in some embodiments of the present application, the mangrove extract is diluted to a concentration of 1%-3% to obtain a mangrove extract solution.

[0041] Exemplarily, in some embodiments of the present application, the mangrove extract is diluted to a mangrove extract solution, including being diluted to a concentration of 1%, 1.2%, 1.5%, 1.8%, 2%, 2.2%, 2.5%, 2.8%, 2.9%, 3%, or a range between any two of the aforementioned values.

[0042] Further, in some embodiments of the present application, the piroctone is diluted to a concentration of 0.5%~1%.

[0043] Exemplarily, in some embodiments of the present application, the piroctone is diluted to a concentration of 0.5%~1%, including being diluted to a concentration of 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, or a range between any two of the aforementioned values.

[0044] Some embodiments of the present application provide a pimple-removing cosmetic, including the composition for inhibiting propionibacterium acnes provided by any of the aforementioned embodiments; or the pimple-removing cosmetic including the composition for inhibiting propionibacterium acnes prepared by any of the aforementioned embodiments.

[0045] In the above technical solution, the pimple-removing cosmetic can effectively improve the pimple-removing effect of the cosmetic by adding the composition for inhibiting propionibacterium acnes; and the pimple-removing active ingredient in the pimple-removing cosmetic is green and natural, and has stable pimple-removing effect, and is green and natural without irritation and side effects, as compared with chemical pimple-removing ingredients such as salicylic acid, azelaic acid, benzoyl peroxide, and retinoids.

[0046] The features and performances of the present application are further described in detail below in combination with embodiments: Embodiment 1 A composition for inhibiting propionibacterium acnes is provided, which is composed of the following ingredients: a mangrove extract solution and a piroctone solution; The mass ratio of the mangrove extract solution and the piroctone solution is 1:1.

[0047] The composition is prepared according to the following steps: (1) Mangrove plants are extracted with alcohol to obtain a mangrove extract; the mangrove extract is then diluted with water to a concentration of 3% by mass percentage to obtain a diluted mangrove extract solution; (2) Piroctone (quaternary ammonium salt-73) is mixed with water to prepare a piroctone solution; the concentration of the piroctone solution is 1% by mass percentage; (3) The mangrove extract solution prepared in step (1) above and the piroctone solution prepared in step (2) above are mixed uniformly at a mass ratio of 1:1.

[0048] Example 2 A composition for inhibiting Propionibacterium acnes is provided, which is composed of the following ingredients: a solution of mangrove extract and a solution of piroctone olamine; The mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 2:1.

[0049] The composition is prepared according to the following steps: (1) Mangrove plants are subjected to alcohol extraction to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 3% by mass percentage to obtain a diluted solution of mangrove extract; (2) Piroctone olamine (quaternary ammonium salt-73) is mixed with water to prepare a solution of piroctone olamine; the concentration of the solution of piroctone olamine is 1% by mass percentage; (3) The solution of mangrove extract prepared in step (1) above and the solution of piroctone olamine prepared in step (2) above are mixed uniformly at a mass ratio of 2:1.

[0050] Example 3 A composition for inhibiting Propionibacterium acnes is provided, which is composed of the following ingredients: a solution of mangrove extract and a solution of piroctone olamine; The mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 1:2.

[0051] The composition is prepared according to the following steps: (1) Mangrove plants are subjected to alcohol extraction to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 3% by mass percentage to obtain a diluted solution of mangrove extract; (2) Piroctone olamine (quaternary ammonium salt-73) is mixed with water to prepare a solution of piroctone olamine; the concentration of the solution of piroctone olamine is 1% by mass percentage; (3) The solution of mangrove extract prepared in step (1) above and the solution of piroctone olamine prepared in step (2) above are mixed uniformly at a mass ratio of 1:2.

[0052] Example 4 A composition for inhibiting Propionibacterium acnes is provided, which is composed of the following ingredients: a solution of mangrove extract and a solution of piroctone olamine; The mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 1:3.

[0053] The composition is prepared according to the following steps: (1), the plant of Manglietia, using alcohol extraction, taking the extract, getting Manglietia extract; with mass percentage, then add water to dilute the Manglietia extract to the concentration of 3%; get the dilution of Manglietia extract solution; (2), prepare piroctone olamine (quaternary ammonium salt-73) and water mixture, prepared piroctone olamine solution; with mass percentage, piroctone olamine solution concentration is 1% piroctone olamine solution; (3) the above step (1) prepared Manglietia extract solution and the above step (2) prepared piroctone olamine solution, according to the mass ratio of 1:3 mixed evenly.

[0054] Example 5 Provided a kind of composition for inhibiting propionibacterium acnes, the composition consists of the following ingredients: Manglietia extract solution and piroctone olamine solution; The mass ratio of Manglietia extract solution and piroctone olamine solution is 3:1.

[0055] The composition is prepared according to the following steps: (1), the plant of Manglietia, using alcohol extraction, taking the extract, getting Manglietia extract; with mass percentage, then add water to dilute the Manglietia extract to the concentration of 3%; get the dilution of Manglietia extract solution; (2), prepare piroctone olamine (quaternary ammonium salt-73) and water mixture, prepared piroctone olamine solution; with mass percentage, piroctone olamine solution concentration is 1% piroctone olamine solution; (3) the above step (1) prepared Manglietia extract solution and the above step (2) prepared piroctone olamine solution, according to the mass ratio of 3:1 mixed evenly.

[0056] Example 6 Provided a kind of composition for inhibiting propionibacterium acnes, the composition consists of the following ingredients: Manglietia extract solution and piroctone olamine solution; The mass ratio of Manglietia extract solution and piroctone olamine solution is 1:4.

[0057] The composition is prepared according to the following steps: (1), the plant of Manglietia, using alcohol extraction, taking the extract, getting Manglietia extract; with mass percentage, then add water to dilute the Manglietia extract to the concentration of 3%; get the dilution of Manglietia extract solution; (2), prepare piroctone olamine (quaternary ammonium salt-73) and water mixture, prepared piroctone olamine solution; with mass percentage, piroctone olamine solution concentration is 1% piroctone olamine solution; (3) the above step (1) prepared Manglietia extract solution and the above step (2) prepared piroctone olamine solution, according to the mass ratio of 1:4 mixed evenly.

[0058] Example 7 A composition for inhibiting propionibacterium acnes is provided, which is composed of: a solution of mangrove extract and a solution of piroctone olamine; The mass ratio of the solution of mangrove extract and the solution of piroctone olamine is 4:1.

[0059] The composition is prepared according to the following steps: (1) Mangrove plants are extracted with alcohol to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 3% by mass percentage; and a solution of the diluted mangrove extract is obtained; (2) Piroctone olamine (quaternary ammonium salt-73) is mixed with water to prepare a solution of piroctone olamine; the concentration of the solution of piroctone olamine is 1% by mass percentage; (3) The solution of mangrove extract prepared in step (1) above is mixed with the solution of piroctone olamine prepared in step (2) above at a mass ratio of 4:1.

[0060] Comparative Example 1 A solution of mangrove extract is provided and prepared according to the following steps: Mangrove plants are extracted with alcohol to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 1% by mass percentage; and a solution of the diluted mangrove extract is obtained.

[0061] Comparative Example 2 A solution of mangrove extract is provided and prepared according to the following steps: Mangrove plants are extracted with alcohol to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 2% by mass percentage; and a solution of the diluted mangrove extract is obtained.

[0062] Comparative Example 3 A solution of mangrove extract is provided and prepared according to the following steps: Mangrove plants are extracted with alcohol to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 3% by mass percentage; and a solution of the diluted mangrove extract is obtained.

[0063] Comparative Example 4 A solution of mangrove extract is provided and prepared according to the following steps: Mangrove plants are extracted with alcohol to obtain mangrove extract; the mangrove extract is then diluted with water to a concentration of 3.5% by mass percentage; and a solution of the diluted mangrove extract is obtained.

[0064] Comparative Example 5 A piroctone olamine solution was provided, which was prepared according to the following steps: Piroctone olamine (quaternary ammonium salt - 73) was prepared by mixing with water to obtain a piroctone olamine solution; the concentration of the piroctone olamine solution was 0.5% by mass percentage.

[0065] Comparative Example 6 A piroctone olamine solution was provided, which was prepared according to the following steps: Piroctone olamine (quaternary ammonium salt - 73) was prepared by mixing with water to obtain a piroctone olamine solution; the concentration of the piroctone olamine solution was 0.8% by mass percentage.

[0066] Comparative Example 7 A piroctone olamine solution was provided, which was prepared according to the following steps: Piroctone olamine (quaternary ammonium salt - 73) was prepared by mixing with water to obtain a piroctone olamine solution; the concentration of the piroctone olamine solution was 1% by mass percentage.

[0067] Comparative Example 8 A piroctone olamine solution was provided, which was prepared according to the following steps: Piroctone olamine (quaternary ammonium salt - 73) was prepared by mixing with water to obtain a piroctone olamine solution; the concentration of the piroctone olamine solution was 1.5% by mass percentage.

[0068] The performance of each sample prepared in the examples and comparative examples in inhibiting propionibacterium acnes was tested.

[0069] Test Example 1 (1) Propionibacterium acnes was activated and a bacterial suspension was prepared, and the bacterial concentration was adjusted to 1.0-2.0*10 6 CFU / mL, 100 μL of the above bacterial suspension was inoculated into a prepared Clostridium difficile agar plate, and a coating rod was used for uniform coating; (2) Preparation of bacteriostatic sheets and control samples, sterile dry filter paper sheets (5 mm in diameter) were taken, 20 μL of the composition solution was added to each sheet, and then placed in a sterile plate, and after drying in a constant temperature oven at 36°C, it was ready for use. The control sample paper sheet was prepared with sterile distilled water; (3) Paste the bacteriostatic sheet, 4 pieces of each example or comparative example test sample and 1 piece of control sample were pasted on each bacterial plate, a total of 5 pieces. The sample sheet was taken with a sterile forceps and pasted on the surface of the plate, the negative control sample was pasted in the center, and the test sample was pasted around the center. The distance between the centers of each sample sheet was more than 25 mm, and the distance from the edge of the plate was more than 15 mm. Cover the plate and place it in a 36°C incubator for 18 hours to observe the results. The test was repeated 3 times; (4) Measure the diameter of the bacteriostatic ring (including the pasted sheet) and record it. When measuring the bacteriostatic ring, completely sterile growth should be selected.

[0070] The test results are shown in Tables 1-3.

[0071] Table 1

[0072] As can be seen from the test results of Table 1 above, the bacteriostatic ring diameters of each of the embodiments are greater than that of the control sample; thus, it can be illustrated that the schemes of each of the embodiments of the present application can effectively improve the bacteriostatic effect.

[0073] Further, the test results (bacteriostatic test results of mangrove extract) of Comparative Examples 1-3 are shown in Table 2: Table 2

[0074] As can be seen from the test results above, the bacteriostatic ring diameters of each of the comparative examples are less than that of each of the embodiments; thus, it can be illustrated that the schemes of each of the embodiments produce a synergistic effect of the components in each of the embodiments, relative to the single mangrove extract, and improve the bacteriostatic effect.

[0075] Further, the test results (bacteriostatic test results of mangrove extract) of Comparative Examples 1-3 are shown in Table 2: Table 3

[0076] As can be seen from the test results above, the bacteriostatic ring diameters of each of the comparative examples are less than that of each of the embodiments; thus, it can be illustrated that the schemes of each of the embodiments produce a synergistic effect of the components in each of the embodiments, relative to the single mangrove extract, and improve the bacteriostatic effect.

[0077] In summary, the components of the composition provided in the embodiments of the present application play a synergistic effect, effectively improving the effect of inhibiting propionibacterium acnes.

[0078] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A composition for inhibiting Propionibacterium acnes, characterized by comprising, consists of: a solution of mangrove extract and a solution of paeonia extract; a mass ratio of the solution of mangrove extract to the solution of paeonia extract is (1-4):(4-1). 2.The composition for inhibiting propionibacterium acnes according to claim 1, wherein, a mass ratio of the solution of mangrove extract to the solution of paeonia extract is 1:

1. 3.The composition for inhibiting propionibacterium acnes according to claim 1, wherein, a concentration of the solution of mangrove extract is 1-3% in mass percentage. 4.The composition for inhibiting propionibacterium acnes according to claim 1, wherein, a concentration of the solution of paeonia extract is 0.5-1% in mass percentage. 5.The composition for inhibiting propionibacterium acnes according to any one of claims 1-4, wherein, the solution of mangrove extract comprises an alcohol extract of mangrove plants. 6.The composition for inhibiting propionibacterium acnes according to claim 5, wherein, the solution of mangrove extract comprises an ethanol extract of mangrove plants. 7.The composition for inhibiting propionibacterium acnes according to claim 5, wherein, a mass ratio of the solution of mangrove extract to the solution of paeonia extract is (1-3):(3-1).

8. A method of preparing a composition for inhibiting P. acnes according to any one of claims 1 to 7, characterized in that, comprising: extracting mangrove plants by alcohol to obtain an extract of mangrove plants; diluting the extract of mangrove plants to obtain a solution of mangrove extract; mixing paeonia with water to obtain a solution of paeonia extract; mixing the solution of mangrove extract and the solution of paeonia extract according to a mass ratio of (1-4):(4-1).

9. A method of preparing a composition for inhibiting P. acnes according to any one of claims 1 to 7, characterized in that, comprising: diluting the extract of mangrove plants to obtain a solution of mangrove extract, including diluting the extract of mangrove plants to a concentration of 1-3%; optionally, mixing paeonia with water to obtain a solution of paeonia extract, including diluting the paeonia to a concentration of 0.5-1%.

10. An anti-acne cosmetic, characterized by, the composition for inhibiting propionibacterium acnes according to any one of claims 1-7; or the anti-acne cosmetic comprises the composition for inhibiting propionibacterium acnes prepared by the preparation method of the composition for inhibiting propionibacterium acnes according to claim 8 or 9.

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