Keemun black tea-root composition with repairing and anti-aging effects as well as preparation method and application of Keemun black tea-root composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a Keemun black tea-root composition with repairing and anti-aging effects as well as a preparation method and application of the Keemun black tea-root composition. The Keemun black tea root extract, the elm root extract and the acanthopanax root extract in a specific amount ratio range are scientifically compounded, the synergistic effect among the components is remarkable, the effect limitation of a single component is effectively overcome, and the synergistic enhancement of anti-aging and repairing effects is realized. Experiments and exploration find that the composition can significantly inhibit the activity of elastase and delay the skin aging process caused by elastin degradation; meanwhile, the transdermal moisture loss can be obviously improved, and the skin barrier function and the self-repairing capability are enhanced. Therefore, the Keemun black tea-root composition disclosed by the invention is outstanding in overall effect, has the characteristics of naturalness, high efficiency and mildness, and accords with the development trend of modern skin care products.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a Qihong tea-root composition with repair and anti-aging effects and a preparation method and application thereof. BACKGROUND

[0002] At present, mainstream anti-aging products mostly rely on chemical synthetic ingredients or single plant extracts. For example, retinol and its derivatives can effectively promote collagen synthesis, but have poor light stability and can easily cause skin redness and irritation, especially for Asian sensitive skin. Chemical antioxidants such as vitamin C derivatives have low bioavailability and need to be used frequently to maintain their effects. In the application of natural extracts, the existing technology mostly focuses on plant stems, leaves or fruit parts, such as tea polyphenols mostly derived from tea leaves rather than tea roots, but ignores the important active ingredient library of plant roots, resulting in low resource utilization and single efficacy.

[0003] As the core organ for absorbing and storing nutrients, plant roots accumulate a wealth of bioactive substances in long-term evolution, and have multiple skin care advantages. Firstly, root extracts are rich in polyphenols, polysaccharides, saponins and other complex components, which can play a role through multiple targets such as antioxidant, anti-inflammatory and collagen synthesis promotion. Secondly, compared with synthetic ingredients, plant root extracts are safer and suitable for various skin types including sensitive skin. For example, peony root extract has the effects of moisturizing and antibacterial, and can gently maintain the water-oil balance of the skin and enhance the natural barrier of the skin, meeting the safety requirements of cosmetic raw materials.

[0004] Although plant root extracts have significant potential, the existing technology has not fully developed their application value. Therefore, it is of important application value to develop a repair and anti-aging composition based on the synergistic effect of multiple plant root extracts, which can not only avoid the defects of traditional chemical ingredients, but also fully exert the complex effects of natural root resources. SUMMARY

[0005] The present application aims to overcome the deficiencies of the prior art and provide a Qihong tea-root composition with repair and anti-aging effects and a preparation method thereof. Through the synergistic effect of the components, the elastase inhibition rate and the improvement rate of transepidermal water loss of the product can be significantly improved, thereby effectively enhancing the anti-aging repair effect of the product.

[0006] To achieve the above purpose, the technical solution adopted by the present application includes:

[0007] In a first aspect, the present application provides a Qihong tea-root composition with repair and anti-aging effects, which comprises Qihong tea root extract, spring elm root extract and acanthopanax root extract.

[0008] Preferably, the Qihong tea-root composition comprises the following components by weight: Qihong tea root extract 2-7 parts, Ulmus japonica root extract 0.5-2 parts, and Acanthopanax senticosus root extract 1-3 parts.

[0009] The Qihong tea root extract is the underground root system part of Qimen tea tree, usually a perennial lignified or semi-lignified structure, in the form of branched rhizome, bearing the functions of absorbing soil nutrients, fixing the plant, and storing part of the nutrients, and generally not used as a direct drinking tea raw material. It contains polysaccharides, triterpenoids and minerals, can moisturize and hydrate the skin, promote skin barrier repair, and has weak antioxidant properties.

[0010] The Acanthopanax senticosus root is the most important medicinal part of Acanthopanax, and its extract is the main raw material of Acanthopanax related products such as medicines and health products, because the active ingredient content of the root is the highest and the efficacy is the most stable. It contains active substances such as acanthopanax saponins and polysaccharides, can regulate skin cell metabolism, enhance cell vitality, assist in delaying skin aging, and promote the expression of barrier repair related proteins.

[0011] Ulmus japonica is a deciduous tree of Ulmaceae Ulmus, and its root extract is an active ingredient complex obtained from the roots of Ulmus japonica through modern extraction technology. It contains various triterpenoids, flavonoids, tannins, polysaccharides and other components, has strong moisturizing and barrier repair ability, can directly improve trans-epidermal water loss, and enhance the skin's water retention function.

[0012] The present application combines Qihong tea root extract, Ulmus japonica root extract and Acanthopanax senticosus root extract to develop a composition with repair and anti-aging effects. The combination of the three within the preferred range of the present application can achieve the synergistic improvement of "anti-aging (maintaining elasticity)" and "repair (strengthening the barrier)", effectively solving the limitation that single component can only cover part of the efficacy. Moreover, the raw materials are all natural plant root extracts, which are safe and suitable for various skin types, especially for early aging and barrier fragile skin, and have a wide application prospect in products.

[0013] Preferably, the Qihong tea-root composition comprises the following components by weight: Qihong tea root extract 3-7 parts, Ulmus japonica root extract 1-2 parts, and Acanthopanax senticosus root extract 1.5-3 parts.

[0014] Preferably, the Qihong tea-root composition comprises the following components by weight: Qihong tea root extract 6 parts, Ulmus japonica root extract 2 parts, and Acanthopanax senticosus root extract 2 parts.

[0015] It is found through experiments that when the three components are matched in the optimal amount defined above, their respective effects can be maximized, thereby ensuring that the prepared composition has excellent effects in repair and anti-aging, and has the best cost performance.

[0016] In a second aspect, the present application provides the use of the Qihongcha-Genus composition in cosmetics.

[0017] Preferably, the cosmetics include water, essence, cream, sunscreen, mask and makeup.

[0018] In a third aspect, the present application provides an essence with anti-aging effect, which comprises the Qihongcha-Genus composition.

[0019] Preferably, the essence further comprises a humectant, a solubilizer and a preservative.

[0020] The humectant comprises at least one of glycerin, 1,3-butanediol, vitamin E and allantoin; and / or the thickening agent comprises xanthan gum; and / or the preservative comprises phenoxyethanol.

[0021] In a fourth aspect, the present application provides a preparation method of the essence, comprising the following steps:

[0022] S1, mixing Qihongcha root extract, spring elm root extract and acanthopanax root extract with water according to a mass ratio of 1:(8-12), heating to 40-60℃, stirring to dissolve, and obtaining an extract solution;

[0023] S2, heating water to 75-85℃, adding part of the humectant and thickening agent, and stirring to obtain a mixed solution;

[0024] S3, cooling the mixed solution to 35-45℃, adding part of the humectant and the extract solution of step S1, stirring to dissolve, adding the preservative, adjusting the pH value to 5.5-6.5, cooling, and filtering to obtain the essence.

[0025] Preferably, step S1 avoids the Qihongcha root extract, spring elm root extract and acanthopanax root extract from caking, and the solubility in the cosmetic formula is not enough, thereby reducing the skin care effect.

[0026] Preferably, the stirring rate of steps S1, S2 and S3 is 350-450 rpm.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] The Qihong tea-root composition provided by this invention scientifically combines Qihong tea root extract, elm root extract, and Acanthopanax senticosus root extract in specific ratios. The synergistic effect among the components is significant, effectively overcoming the limitations of single-ingredient efficacy and achieving a synergistic enhancement of anti-aging and repair effects. Experimental studies have shown that the composition can significantly inhibit elastase activity, delaying the skin aging process caused by elastin degradation; simultaneously, it can significantly improve transepidermal water loss, enhancing skin barrier function and self-repair ability. Therefore, the Qihong tea-root composition of this invention has outstanding overall efficacy, combining natural, high-efficiency, and gentle characteristics, aligning with the development trend of modern skincare products. Attached Figure Description

[0029] Figure 1 The image shows a comparison of the effects of the serums prepared in the examples and comparative examples on inhibiting elastase. Detailed Implementation

[0030] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0031] The following examples and comparative examples show that the *Ulmus pumila* root extract was purchased from Fufeng Snowt Biotechnology Co., Ltd.; the *Acanthopanax senticosus* root extract was purchased from Hunan Langlin Biological Resources Co., Ltd.; and the *Qihong* tea root extract and *Qihong* tea leaf extract, taken from the leaves, were both purchased from Qimen Xie Yongzhong Black Tea Development Co., Ltd. The *Ulmus pumila* leaf extract, taken from the leaves, was purchased from Ningxia Xiangcao Biotechnology Co., Ltd. The *Acanthopanax senticosus* stem extract, taken from the stem, was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0032] Examples 1-5

[0033] Examples 1-5 provide a serum with repairing and anti-aging effects, the formula of which is shown in Table 1 (total weight parts: 100 parts). The preparation method of the serum includes the following steps:

[0034] S1. The root extracts of Qihong tea, spring elm root, and Acanthopanax senticosus root were sieved through a 100-mesh sieve, and then mixed with deionized water at a mass ratio of 1:10. The mixtures were heated to 50°C and stirred until completely dissolved to obtain three extract solutions.

[0035] S2. Add deionized water to a mixing pot and heat to 80°C. Add glycerin and 1,3-butanediol in sequence and stir until completely dissolved. Then add xanthan gum and continue stirring until the xanthan gum is fully swollen and the solution is uniform and transparent.

[0036] S3, cooling to 40℃, adding allantoin, vitamin E and the solution of the three extracts of step S1, stirring until completely dissolved, then adding chlorphenesin, adjusting pH to 6 with citric acid, cooling, filtering, standing, to obtain the serum.

[0037] Table 1 Formulation table of the serum of Examples 1-5 (weight parts)

[0038]

[0039]

[0040] Comparative Examples 1-7

[0041] Comparative Examples 1-7 provide a serum, the formulation table of which is shown in Table 2 (total weight parts are 100 parts). The preparation method of the serum is the same as that of Example 1, with the only difference being the formulation.

[0042] Table 2 Formulation table of the serum of Comparative Examples 1-7 (weight parts)

[0043] Comparative Example 8

[0044] This comparative example provides a serum, which is different from Example 1 only in that an equal amount of Qihong tea leaf extract is used to replace Qihong tea root extract, and the amounts of the other components and the preparation method are the same as those of Example 1.

[0045] Comparative Example 9

[0046] This comparative example provides a serum, which is different from Example 1 only in that an equal amount of spring elm leaf extract is used to replace spring elm root extract, and the amounts of the other components and the preparation method are the same as those of Example 1.

[0047] Comparative Example 10

[0048] This comparative example provides a serum, which is different from Example 1 only in that an equal amount of Acanthopanax senticosus stem extract is used to replace Acanthopanax senticosus root extract, and the amounts of the other components and the preparation method are the same as those of Example 1.

[0049] Effect Example 1

[0050] This effect example takes the serum prepared in Examples 1-5 and Comparative Examples 1-10 as test samples, and tests their anti-aging effects, with the specific method being as follows:

[0051] (1) Reagents and materials

[0052] 1. 30 U / mg elastase (porcine pancreatic trypsin), N-succinyl-alanine-alanine-alanine-p-nitroaniline, Tris, concentrated hydrochloric acid and EGCG;

[0053] 2. 0.2 mol / L Tris-HCl buffer solution (buffer) with pH = 8.0 was prepared:

[0054] Mixture A: 4.8456 g of Tris particles was accurately weighed, added to 100 mL of deionized water, stirred and dissolved to obtain a 0.4 mol / L Tris solution; Mixture B: 3.34 mL of concentrated hydrochloric acid was diluted with 96.66 mL of deionized water to obtain a 0.4 mol / L HCl solution; 100 mL of Mixture A and 44.76 mL of Mixture B were mixed, the pH was adjusted to 8.0, and deionized water was added to make up to 200 mL;

[0055] 3. Elastase solution (enzyme solution): prepared with the buffer, 0.033 g of elastase was added to 10 mL of the buffer to prepare a 100 U / mL elastase solution, which was prepared immediately before use;

[0056] 4. Substrate solution: 1.8057 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline was weighed and dissolved in 10 mL of the buffer to obtain a 0.18057 mg / mL N-succinyl-alanine-alanine-alanine-p-nitroaniline solution;

[0057] 5. Positive control polypeptide solution: 0.001 g of polypeptide powder was weighed and dissolved in 1 mL of the buffer to prepare a 1.0 mg / mL polypeptide solution, which was diluted with the buffer to obtain 0.5, 0.1, 0.01, 0.005, 0.003, 0.002 and 0.001 mg / mL polypeptide solutions in turn.

[0058] (2) Determination steps

[0059] Each solution was added to a 96-well plate in turn according to Table 3, and incubated at room temperature for 60 min. The absorbance was detected at 410 nm using an enzyme marker, and the data was recorded and saved. The absolute difference between two independent determination results obtained under repeatable conditions should not exceed 10% of the arithmetic mean. The elastase inhibition rate (%) was calculated according to formula (1), and the results are shown in Table 4.

[0060] Elastase inhibition rate (%) = [1- (OD A - OD B ) / (OD C - OD D )] x 100% (Formula 1)

[0061] In the above formula, OD AAbsorbance value of sample group (containing enzyme and substrate); OD B Absorbance value of sample control group (containing enzyme and no substrate); OD C Absorbance value of blank group (containing enzyme and substrate and no sample); OD D Absorbance value of blank control group (containing enzyme and no substrate and no sample).

[0062] Table 3 Test liquid composition

[0063] Composition / (μL) Sample solution Enzyme solution Substrate solution Buffer Blank group / 50 100 50 Blank control group / 50 / 150 Sample group 50 50 100 / Sample control group 50 50 / 100

[0064] Table 4 Elastase inhibition rate results of different test samples

[0065]

[0066] Elastase inhibition rate is an important indicator for evaluating anti-aging effect. Excessive activity of elastase can decompose skin elastin, leading to skin relaxation and aging. The higher the inhibition rate, the stronger the anti-aging potential. The results in Table 4 show that the essence containing the Qi Hong tea-root composition prepared by the technical solution of the present application can effectively inhibit the activity of elastase, thereby exerting a good anti-aging effect. In particular, the essence prepared in Example 1 has the best anti-aging effect.

[0067] However, the elastase inhibition rate of the essence containing only one of the three active components of Qi Hong tea root extract, spring elm root extract and acanthopanax root extract in Comparative Examples 1-3 is significantly lower than that of the examples, indicating that the three root extracts cannot effectively inhibit elastase when used alone, and the anti-aging effect is weak, and the effect needs to rely on combination. In Comparative Examples 4-5, which contain only two of the above active components, the elastase inhibition rate is higher than that of a single component, but still significantly lower than that of the examples, indicating that the combination of two root extracts can produce a certain synergistic effect, but when the third component is lacking, the synergistic effect cannot be fully exerted, and the anti-aging effect is still not ideal, further proving that the presence of the three specific components together is a necessary condition for achieving high-efficiency anti-aging. In Comparative Examples 6-7, the amount ratio of the three active components exceeds the preferred range of the present application, and the elastase inhibition rate of the prepared essence is also lower than that of the examples, indicating that the ratio of the three root extracts significantly affects the synergistic effect. Only within the preferred ratio range of the present application can the elastase inhibition capacity be maximized. Once the ratio deviates, the anti-aging effect will be directly weakened. In Comparative Examples 8-10, Qi Hong tea leaf extract, spring elm leaf extract and acanthopanax stem extract are used to replace the corresponding root extracts, and the inhibition rates are also lower than those of the examples, indicating that the root part extracts of the three plants have higher activity than the non-root part extracts in inhibiting elastase. The root part contains more anti-aging active ingredients, which is an important source for the composition to exert effect. Replacing the root part extract with other part extracts of the plant will reduce the anti-aging effect.

[0068] Example 2

[0069] In this effect example, human repair effect test was conducted on samples of Examples 1-5 and Comparative Examples 1-10.

[0070] 150 volunteers were recruited and divided into 15 groups, with 10 people in each group, and the volunteers applied the serum obtained from Examples 1-5 and Comparative Examples 1-10, respectively, and the transepidermal water loss rate (TEWL) of the volunteers' skin was tested by using a skin moisture loss tester Tewameter R TM 300.

[0071] The selection criteria of the volunteers included: healthy women, aged 30-60 years old, with sensitive skin (positive lactic acid stinging reaction), dry and red skin on the face, poor skin barrier function in the test area (TEWL≥15 g / m 2 / h), able to read and understand all the contents of the informed consent form, willing to sign the informed consent form, agreeing not to use any cosmetics, drugs and health products that may affect the results during the test period, not participating in any other clinical trials in other research centers during the test period, and not participating in any other clinical studies in the past month.

[0072] The volunteers were ensured to have not used antihistamines in the past week or immunosuppressants in the past month, not used any anti-inflammatory drugs in the test area in the past two months, not suffered from inflammatory skin diseases or suffered from inflammatory skin diseases but clinically cured, not suffered from other chronic respiratory diseases such as diabetes or asthma, not in lactation or pregnancy, not undergone bilateral mastectomy or bilateral axillary lymph node dissection, not received anti-cancer chemotherapy in the past 6 months, not allergic, not in the treatment of dermatology, not taken hydroxy acid, whitening and anti-aging drugs in the past month, and the test area was free of large areas of birthmarks, scratches, vitiligo, pigmented nevi, keloid scars and other skin manifestations that may affect the test.

[0073] During the test period, the volunteers should pay attention to the following matters: maintain the same lifestyle and dietary habits as before participating in the test, prohibit any treatment that may affect the test on the tested area, prohibit the use of other skin care products and other products that may affect the test during the test, and use daily necessities consistent with those used before the start of the test and prohibit replacement.

[0074] Before starting to use the samples, the TEWL value of the volunteers was tested (this value is recorded as W0), and 1-2 g of the sample was evenly applied to the face after cleansing in the morning and evening, and then gently massaged and absorbed. After 4 weeks of continuous use, the TEWL value was tested (this value is recorded as W4). Before testing the TEWL value, the volunteers should use clean water to clean their faces, sit still for 30 minutes in a constant temperature and humidity environment, and then start testing. The TEWL detection results are shown in Table 5.

[0075] Table 5 TEWL detection results of different serums

[0076]

[0077] The trans-epidermal water loss (TEWL) improvement rate is a core objective quantification index of skin repair effect, the higher the TEWL improvement rate, the more complete the skin barrier function recovery, the stronger the skin water locking ability, defense ability and stability, and the more significant the repair effect. The results in Table 5 show that the essence containing the technical solution prepared by the application of the Qihong tea-root composition can effectively improve the trans-epidermal water loss and achieve a better repair effect, and compared with the trans-epidermal water loss improvement rate of the essence prepared by the comparative example, it can be known that when the Qihong tea root extract, spring elm root extract and acanthopanax senticosus root extract are combined in the preferred ratio of the application, the strongest repair synergistic effect can be produced, and the trans-epidermal water loss improvement ability is far superior to that of single component, two component combination or ratio deviating from the preferred range.

[0078] Finally, it should be noted that the above examples are to illustrate the technical solutions of the application rather than limit the protection scope of the application, although the application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the application.

Claims

1. A Qihong tea-root composition with repairing and anti-aging effects, characterized in that, The Qihong tea-root composition includes Qihong tea root extract, elm root extract, and Acanthopanax senticosus root extract.

2. The Qihong tea-root composition as described in claim 1, characterized in that, The Qihong tea-root composition comprises the following components in parts by weight: 2-7 parts of Qihong tea root extract, 0.5-2 parts of elm root extract, and 1-3 parts of Acanthopanax senticosus root extract.

3. The Qihong tea-root composition as described in claim 2, characterized in that, The Qihong tea-root composition comprises the following components in parts by weight: 3-7 parts of Qihong tea root extract, 1-2 parts of spring elm root extract, and 1.5-3 parts of Acanthopanax senticosus root extract.

4. The Qihong tea-root composition as described in claim 3, characterized in that, The Qihong tea-root composition comprises the following components in parts by weight: 6 parts Qihong tea root extract, 2 parts spring elm root extract, and 2 parts Acanthopanax senticosus root extract.

5. The use of the Qihong tea-root composition as described in any one of claims 1-4 in cosmetics.

6. A serum with repairing and anti-aging effects, characterized in that, The essence comprises the Qihong tea-root composition according to any one of claims 1-4.

7. The essence as described in claim 6, characterized in that, The serum also includes moisturizers, thickeners, and preservatives.

8. The essence as described in claim 7, characterized in that, The humectant includes at least one of glycerin, 1,3-butanediol, vitamin E, and allantoin; and / or the thickener includes xanthan gum; and / or the preservative includes phenoxyethanol.

9. The method for preparing the essence as described in claim 7 or 8, characterized in that, Includes the following steps: S1. Mix the root extracts of Qihong tea, the root extract of spring elm, and the root extract of Acanthopanax senticosus with water at a mass ratio of 1:(8-12), heat to 40-60℃, and stir until dissolved to obtain the extract solution. S2. Heat the water to 75-85℃, add some humectant and thickener to obtain a mixed solution; S3. Cool the mixed solution to 35-45℃, add some moisturizer and the extract solution from step S1, stir until dissolved, then add preservative, adjust the pH to 5.5-6.5, cool, and filter to obtain the essence.

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