Slimming composition containing reed extract and preparation method thereof

Abstract

The invention provides a slimming composition containing a reed extract, which is prepared from the following components in parts by weight: 1 to 8 parts of the reed extract, 1 to 5 parts of a coffee seed extract, 1 to 5 parts of a zingiber zingiberensis extract, 1 to 10 parts of 1, 2-pentanediol, 0.5 to 3 parts of water-soluble azone and 0.5 to 3 parts of phosphatidylcholine. Wherein the weight ratio of the reed extract to the coffee seed extract to the zingiber zingiberensis extract is (1-8): (1-5): (1-5), and the weight ratio of the 1, 2-pentanediol to the phosphatidylcholine is (1-10): (0.5-3). The invention also provides a preparation method of the slimming composition. The slimming composition containing the reed extract, provided by the invention, has relatively good fat-reducing and slimming effects.

Description

Technical Field

[0001] This invention relates to a slimming composition, and more particularly to a slimming composition containing reed extract and its preparation method. Background Technology

[0002] As people's living standards improve, the pace of life accelerates, and the frequency of exercise decreases, the number of obese people is gradually increasing. This is because the energy intake and utilization in the body are in a long-term imbalance, resulting in surplus energy that is stored as fat and causes obesity. To address the problem of obesity, the cosmetics field has made some research achievements, but the designed functional pathways are relatively simple, resulting in poor effects or slow onset of action.

[0003] Chinese patent CN112057385A discloses an almond slimming cream, its composition, and its preparation method, comprising 18-45 parts by weight of almond oil and 1.3-3.4 parts by weight of rhubarb extract. This invention achieves slimming and skin-tightening effects in a relatively safe and gentle manner, but its mechanism of action is singular and its effects are not obvious. Chinese patent CN116869877A discloses a firming and shaping lotion containing longan seed extract and its preparation method, comprising water, carbomer, caffeine, glycerin, panthenol, tocopheryl acetate, coffee seed extract, lecithin, and longan seed extract, among other ingredients. The fat-reducing pathway of this invention is also relatively singular, mainly relying on caffeine and lecithin to promote fat metabolism and improve blood circulation, without considering cellular lipid absorption or inflammation of fat cells, thus failing to achieve significant slimming effects. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a slimming composition containing reed extract with good fat reduction and slimming effect.

[0005] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0006] A slimming composition containing reed extract comprises the following components in parts by weight: 1-8 parts reed extract, 1-5 parts small-fruit coffee seed extract, 1-5 parts red ginger extract, 1-10 parts 1,2-pentanediol, 0.5-3 parts water-soluble azone, and 0.5-3 parts phosphatidylcholine; wherein the weight ratio of reed extract, small-fruit coffee seed extract, and red ginger extract is (1-8):(1-5):(1-5), and the weight ratio of 1,2-pentanediol and phosphatidylcholine is (1-10):(0.5-3).

[0007] Furthermore, the present invention comprises the following components in parts by weight: 5 parts of reed extract, 3 parts of small-fruited coffee seed extract, 3 parts of red ginger extract, 5 parts of 1,2-pentanediol, 2 parts of water-soluble azone, and 2 parts of phosphatidylcholine.

[0008] Furthermore, the preparation steps of the reed extract of the present invention are as follows:

[0009] Reed fruits were pulverized and dried to obtain reed fruit powder. The reed fruit powder was added to an ethanol-water solution, stirred for 30 minutes, and then ultrasonically extracted at 20-40℃ for 1 hour to obtain an extract. The extract was centrifuged for 30 minutes, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Propylene glycol was added to the concentrate, stirred evenly, and impurities were filtered off to obtain the reed extract. The dry matter content of the reed extract was greater than 9%, of which saponins accounted for 10-40%, flavonoids accounted for 1-6%, and polyphenols accounted for 2-15%. Cyclic adenosine monophosphate (cAMP) is an intracellular signaling molecule that participates in regulating metabolism and biological functions. It can trigger the lipolysis cascade by inhibiting lipogenesis and stimulating lipase activity. The main function of phosphodiesterase III is to degrade cyclic adenosine monophosphate (cAMP), affecting intracellular signal transduction pathways. The reed extract prepared by this invention has the effect of inhibiting phosphodiesterase III and increasing the level of cyclic adenosine monophosphate (cAMP), which can activate lipolysis and thus consume subcutaneous fat reserves. At the same time, the flavonoids and polyphenols contained in the reed extract can expel excess water from fat cells and improve the activity of lipolysis.

[0010] Furthermore, in the preparation steps of the reed extract of the present invention: the particle size of the reed fruit powder is 50 mesh; the volume ratio of ethanol to water in the ethanol aqueous solution is 2:1, and the mass ratio of reed fruit powder to ethanol aqueous solution is 1:10; the ultrasonic power during ultrasonic extraction is 500W; the centrifugation speed during centrifugation is 3000r / min; and the volume ratio of concentrate to propylene glycol is 1:5.

[0011] Furthermore, the preparation steps of the small-fruit coffee seed extract of the present invention are as follows:

[0012] Coffee seeds were pulverized and dried to obtain coffee seed powder. The coffee seed powder was added to an acetone aqueous solution and stirred for 30 minutes. Then, it was ultrasonically extracted at 20-40°C for 1 hour to obtain an extract. The extract was centrifuged for 30 minutes, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain coffee seed extract. In addition to caffeine, which has lipolytic activity, coffee seed extract also contains a large amount of cafestol and coffee bean alcohol, which have structures similar to cholesterol and can achieve similar physiological effects. Experiments have shown that free cholesterol can inhibit the synthesis of cellular lipoprotein receptors. Therefore, the coffee seed extract obtained in this invention has the effect of inhibiting the synthesis of LDL and VLDL lipid receptors on adipocyte membranes, effectively preventing lipid molecules from entering adipocytes, reducing fat storage levels, and thus reducing adipocyte volume, achieving a fat reduction effect.

[0013] Furthermore, in the preparation steps of the coffee seed extract of the present invention: the particle size of the coffee seed powder is 50 mesh; the volume ratio of acetone to water in the acetone aqueous solution is 2:1, and the mass ratio of coffee seed powder to propanol aqueous solution is 1:10; the ultrasonic power during ultrasonic extraction is 500W; the centrifugation speed during centrifugation is 3000r / min; and the volume ratio of concentrate to propylene glycol is 1:5.

[0014] Furthermore, the preparation steps of the red ginger extract of the present invention are as follows:

[0015] Red ginger root was pulverized and dried to obtain red ginger root powder. The red ginger root powder was added to a supercritical extraction vessel, and after introducing carbon dioxide fluid, extraction was carried out at 25-35°C for 2 hours to obtain an extract. The extract was added to a supercritical separation vessel and separated under reduced pressure at 35°C for 1 hour to obtain a separate. The separate was added to water, distilled for 2 hours, and the steam was condensed and recovered to obtain red ginger extract. Each 100g of red ginger extract contains 400-700ppm gingerone. There is a negative cycle between excessive adipose tissue and inflammation of adipocytes. When adipocytes grow excessively and enlarge abnormally, excessive pressure is generated, blood flow slows down, and tissue is compressed, resulting in inflammation. Inflammation of adipocytes, in turn, leads to further enlargement of adipocytes. The red ginger extract prepared in this invention can effectively reduce macrophage activity, alleviate adipocyte inflammation, protect the dermis from the effects of proteases, maintain the integrity of the extracellular matrix of adipocytes, and inhibit the expansion of adipose tissue.

[0016] Furthermore, in the preparation steps of the red ginger extract of the present invention: the particle size of the red ginger root powder is 50 mesh; the flow rate of carbon dioxide in the supercritical extraction vessel is 10 kg / h, the extraction pressure is 25 MPa; and the pressure of the supercritical separation vessel is 4 MPa.

[0017] 1,2-Pentanediol has good moisturizing properties and is safe to use. It is a highly efficient solvent in cosmetics. When used in combination with phosphatidylcholine, it can better disperse and dissolve the active ingredients, effectively enhance their efficacy, improve bioavailability, and maximize their effectiveness.

[0018] Phosphatidylcholine is a phospholipid containing unsaturated fatty acids, with a purity greater than 94%, derived from natural soybeans or sunflowers. It is also a major component of living cell membranes and possesses complete biocompatibility. Its unsaturated nature allows it to penetrate deeper into the dermis and more easily form liposomes, facilitating the penetration of active substances into the skin.

[0019] Water-soluble azone is a modified oil-soluble azone obtained by compounding PPG-5-cetearearyl alcohol polyether-20 and PEG-40 hydrogenated castor oil and butylene glycol. It exhibits significant transdermal penetration enhancement effects on both lipophilic and hydrophilic active ingredients. It can lower the phase transition temperature of active substances into lipids in the stratum corneum intercellular space, increase their fluidity, reduce diffusion resistance of active substances in the stratum corneum, and thus improve absorption of active substances.

[0020] Another technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned slimming composition containing reed extract.

[0021] To solve the above technical problems, the technical solution is as follows:

[0022] A method for preparing a slimming composition containing reed extract includes the following steps:

[0023] S1. Mix 1,2-pentanediol and phosphatidylcholine, and stir at 75-80°C until homogeneous to obtain mixture A;

[0024] S2. Mix coffee seed extract and ginger extract, and stir at 40-45°C until homogeneous to obtain mixture B;

[0025] S3. Add the reed extract to the mixture A obtained in step S1, and stir at 55-60°C until the mixture is homogeneous to obtain mixture C;

[0026] S4. Mix the mixture B obtained in step S2 and the mixture C obtained in step S3, homogenize for 4-6 minutes, and then cool to 40°C to obtain mixture D;

[0027] S5. Add the mixture D obtained in step S4 into a high-pressure homogenizer, turn on the constant temperature water bath to 40°C, and perform three cycles of high-pressure homogenization and emulsification to obtain mixture E.

[0028] S6. Add the mixture E obtained in step S5 into a microfluidic homogenizer, perform microfluidic homogenization once, cool to room temperature, add water-soluble azone and stir until uniformly mixed to obtain a slimming composition containing reed extract.

[0029] Further, in step S4 of the present invention, the homogenization speed is 2500-3500 r / min; in step S5, the pressure of the high-pressure homogenizer is 500-1500 bar; and in step S6, the pressure of the microjet homogenizer is 800-1800 bar.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] This invention utilizes reed extract, coffee seed extract, and ginger extract, each acting through different pathways. First, coffee seed extract inhibits lipid receptor expression on adipocyte membranes, reducing lipid storage levels in adipocytes. Second, ginger extract alleviates inflammation in overgrown adipocytes, preventing further enlargement. Finally, reed extract, rich in flavonoids and polyphenols, activates lipolysis, eliminates excess water from adipocytes, and depletes subcutaneous fat reserves. These three components work synergistically, addressing the root causes of adipocyte hypertrophy and obesity. Furthermore, this invention combines phosphatidylcholine and 1,2-pentanediol to form liposomes, enhancing the transport of active substances. Finally, it works synergistically with water-soluble azone to temporarily disrupt the lipid structure in the stratum corneum, further enhancing the penetration of the active ingredients and maximizing their reach into the subcutaneous fat layer to achieve significant fat reduction and weight loss. Detailed Implementation

[0032] The present invention will now be described in detail with reference to specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0033] Example 1

[0034] The slimming composition containing reed extract consists of the following components in parts by weight: 1 part reed extract, 1 part small-fruit coffee seed extract, 1 part red ginger extract, 1 part 1,2-pentanediol, 0.5 parts water-soluble azone, and 0.5 parts phosphatidylcholine.

[0035] The preparation steps of reed extract are as follows:

[0036] The reed fruits were crushed and dried to obtain reed fruit powder with a particle size of 50 mesh. The reed fruit powder was added to an ethanol-water solution (ethanol and water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 20℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, and the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain the reed extract.

[0037] The preparation steps for small-fruit coffee seed extract are as follows:

[0038] Coffee seeds were pulverized and dried to obtain coffee seed powder with a particle size of 50 mesh. The coffee seed powder was added to an acetone aqueous solution (acetone to water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 20℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain coffee seed extract.

[0039] The preparation steps of red ginger extract are as follows:

[0040] Red ginger root was pulverized and dried to obtain red ginger root powder with a particle size of 50 mesh. The red ginger root powder was added to a supercritical extraction vessel, and carbon dioxide fluid at a flow rate of 10 kg / h was introduced. Extraction was carried out at 25°C and 25 MPa pressure for 2 hours to obtain an extract. The extract was added to a supercritical separation vessel and separated under reduced pressure at 35°C and 4 MPa pressure for 1 hour to obtain a separate. The separate was added to water, distilled for 2 hours, and the steam was condensed and recovered to obtain red ginger extract. Another technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned slimming composition containing reed extract.

[0041] The preparation method of Example 1 includes the following steps:

[0042] S1. Mix 1,2-pentanediol and phosphatidylcholine, and stir at 75°C until homogeneous to obtain mixture A;

[0043] S2. Mix coffee seed extract and ginger extract, and stir at 40°C until homogeneous to obtain mixture B;

[0044] S3. Add the reed extract to mixture A obtained in step S1, and stir at 55°C until the mixture is homogeneous to obtain mixture C;

[0045] S4. Mix the mixture B obtained in step S2 and the mixture C obtained in step S3, homogenize at 2500 r / min for 6 min, and then cool to 40℃ to obtain mixture D;

[0046] S5. Add the mixture D obtained in step S4 into a high-pressure homogenizer with a pressure of 500 bar, turn on the constant temperature water bath to 40°C, and perform three cycles of high-pressure homogenization and emulsification to obtain mixture E.

[0047] S6. Add the mixture E obtained in step S5 to a microjets homogenizer with a pressure of 800 bar, perform microjets homogenization once, cool to room temperature, add water-soluble azone and stir until the mixture is uniform to obtain a slimming composition containing reed extract.

[0048] Example 2

[0049] The slimming composition containing reed extract consists of the following components in parts by weight: 5 parts reed extract, 3 parts small-fruit coffee seed extract, 3 parts red ginger extract, 5 parts 1,2-pentanediol, 2 parts water-soluble azone, and 2 parts phosphatidylcholine.

[0050] The preparation steps of reed extract are as follows:

[0051] The reed fruits were crushed and dried to obtain reed fruit powder with a particle size of 50 mesh. The reed fruit powder was added to an ethanol-water solution (ethanol and water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 30℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, and the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain the reed extract.

[0052] The preparation steps for small-fruit coffee seed extract are as follows:

[0053] Coffee seeds were pulverized and dried to obtain coffee seed powder with a particle size of 50 mesh. The coffee seed powder was added to an acetone aqueous solution (acetone to water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 30℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, and the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain coffee seed extract.

[0054] The preparation steps of red ginger extract are as follows:

[0055] Red ginger root was pulverized and dried to obtain red ginger root powder with a particle size of 50 mesh. The red ginger root powder was added to a supercritical extraction vessel, and carbon dioxide fluid at a flow rate of 10 kg / h was introduced. Extraction was carried out at 30°C and 25 MPa pressure for 2 hours to obtain an extract. The extract was added to a supercritical separation vessel and separated under reduced pressure at 35°C and 4 MPa pressure for 1 hour to obtain a separate. The separate was added to water, distilled for 2 hours, and the steam was condensed and recovered to obtain red ginger extract. Another technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned slimming composition containing reed extract.

[0056] The preparation method of Example 2 includes the following steps:

[0057] S1. Mix 1,2-pentanediol and phosphatidylcholine, and stir at 78°C until homogeneous to obtain mixture A;

[0058] S2. Mix coffee seed extract and ginger extract, and stir at 42°C until homogeneous to obtain mixture B;

[0059] S3. Add the reed extract to mixture A obtained in step S1, and stir at 57°C until the mixture is homogeneous to obtain mixture C;

[0060] S4. Mix the mixture B obtained in step S2 and the mixture C obtained in step S3, homogenize at 3000 r / min for 5 min, and then cool to 40℃ to obtain mixture D;

[0061] S5. Add the mixture D obtained in step S4 into a high-pressure homogenizer with a pressure of 1000 bar, turn on the constant temperature water bath to 40°C, and perform three cycles of high-pressure homogenization and emulsification to obtain mixture E.

[0062] S6. Add the mixture E obtained in step S5 to a microjets homogenizer with a pressure of 1300 bar, perform microjets homogenization once, cool to room temperature, add water-soluble azone and stir until uniformly mixed to obtain a slimming composition containing reed extract.

[0063] Example 3

[0064] The slimming composition containing reed extract consists of the following components in parts by weight: 8 parts reed extract, 5 parts small-fruit coffee seed extract, 5 parts red ginger extract, 10 parts 1,2-pentanediol, 3 parts water-soluble azone, and 3 parts phosphatidylcholine.

[0065] The preparation steps of reed extract are as follows:

[0066] The reed fruits were crushed and dried to obtain reed fruit powder with a particle size of 50 mesh. The reed fruit powder was added to an ethanol-water solution (ethanol and water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 40℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, and the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain the reed extract.

[0067] The preparation steps for small-fruit coffee seed extract are as follows:

[0068] Coffee seeds were pulverized and dried to obtain coffee seed powder with a particle size of 50 mesh. The coffee seed powder was added to an acetone aqueous solution (acetone to water volume ratio of 2:1) at a mass ratio of 1:10. After stirring for 30 min, the mixture was ultrasonically extracted at 40℃ and 500W ultrasonic power for 1 h to obtain an extract. The extract was centrifuged at 3000 r / min for 30 min, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Five times the volume of propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain coffee seed extract.

[0069] The preparation steps of red ginger extract are as follows:

[0070] Red ginger root was pulverized and dried to obtain red ginger root powder with a particle size of 50 mesh. The red ginger root powder was added to a supercritical extraction vessel, and carbon dioxide fluid at a flow rate of 10 kg / h was introduced. Extraction was carried out at 35°C and 25 MPa pressure for 2 hours to obtain an extract. The extract was added to a supercritical separation vessel and separated under reduced pressure at 35°C and 4 MPa pressure for 1 hour to obtain a separate. The separate was added to water, distilled for 2 hours, and the steam was condensed and recovered to obtain red ginger extract. Another technical problem to be solved by the present invention is to provide a method for preparing the above-mentioned slimming composition containing reed extract.

[0071] The preparation method of Example 3 includes the following steps:

[0072] S1. Mix 1,2-pentanediol and phosphatidylcholine, and stir at 80°C until homogeneous to obtain mixture A;

[0073] S2. Mix coffee seed extract and ginger extract, and stir at 45°C until homogeneous to obtain mixture B;

[0074] S3. Add the reed extract to mixture A obtained in step S1, and stir at 60°C until the mixture is homogeneous to obtain mixture C;

[0075] S4. Mix the mixture B obtained in step S2 and the mixture C obtained in step S3, homogenize at 3500 r / min for 4 min, and then cool to 40℃ to obtain mixture D;

[0076] S5. Add the mixture D obtained in step S4 into a high-pressure homogenizer with a pressure of 1500 bar, turn on the constant temperature water bath to 40°C, and perform three cycles of high-pressure homogenization and emulsification to obtain mixture E.

[0077] S6. Add the mixture E obtained in step S5 to a microjets homogenizer with a pressure of 1800 bar, perform microjets homogenization once, cool to room temperature, add water-soluble azone and stir until the mixture is uniform to obtain a slimming composition containing reed extract.

[0078] Comparative Example 1

[0079] The difference from Example 2 is that the components do not include reed extract.

[0080] Comparative Example 2

[0081] The difference from Example 2 is that the components do not include small-fruit coffee seed extract.

[0082] Comparative Example 3

[0083] The difference from Example 2 is that the components do not include red ginger extract.

[0084] Comparative Example 4

[0085] The difference from Example 2 is that the components do not include phosphatidylcholine and 1,2-pentanediol.

[0086] Comparative Example 5

[0087] The difference from Example 2 is that the components do not include water-soluble nitrogen ketones.

[0088] Experimental example:

[0089] Examples 1-3, Comparative Examples 1-5, and Control Examples (blank control) were added to cosmetics for testing. The basic formula of the cosmetics is shown in Table 1. All raw materials used were commercially available products purchased from relevant manufacturers or commercial departments.

[0090] Table 1

[0091]

[0092] The above cosmetics are prepared according to the following steps:

[0093] (1) Weigh each component accurately according to the proportions;

[0094] (2) Heat phase A to 85°C and stir to dissolve to obtain phase A;

[0095] (3) Heat the B phase component to 85°C and stir to dissolve it to obtain the B phase;

[0096] (4) Add phase B to phase A, stir homogenously until uniform, and cool to 40°C;

[0097] (5) Add the C phase component (i.e. the composition obtained in Examples 1-3 and Comparative Examples 1-5) and stir until homogeneous.

[0098] Test 1: Mildness Test

[0099] 1. Test basis: Cosmetic Safety Technical Specifications 2015 Edition, Human Skin Patch Test.

[0100] 2. Test method: 24-hour closed patch test.

[0101] 3. Test subjects: 32 healthy volunteers, aged 20-59, with no history of allergies.

[0102] 4. Testing Procedure: Select an area not exceeding 50mm² 2 A suitable spot treatment device with a depth of approximately 1 mm was used. 0.020-0.025 mL of each of Examples 1-3, Comparative Examples 1-5, and Control Examples were weighed into the device. The subject's hands were cleaned, and the spot treatment device containing the above product was applied to the flexor side of the subject's forearm for 24 hours. The subject was advised to keep the patch area dry for 24 hours and avoid strenuous exercise, scratching, and prolonged sun exposure. After 24 hours, the spot treatment device was removed and marked. After 30 minutes, once the indentation disappeared, the skin reaction was assessed under sufficient light. The grading criteria for adverse skin reactions are shown in Table 2, and the results of adverse skin reactions for each product are shown in Table 3.

[0103] Table 2. Grading Standards for Adverse Skin Reactions

[0104]

[0105] Table 3. Sample patch test results

[0106]

[0107] As can be seen from Table 3, the test results of the skin patch experiment were all negative, indicating that Examples 1-3 of the present invention did not have any serious adverse reactions on the skin, and the slimming composition prepared by the present invention has good safety.

[0108] Test 2: Slimming Efficacy Test

[0109] 1. Test subjects: individuals aged 24-50 years with a body fat percentage (BMI) of 25-32 and who have maintained a stable weight for 3 months; those without allergic diseases or a history of allergies to cosmetics or other topical preparations; those who can understand the testing process and are willing to participate in the trial; a total of 90 participants, divided into 9 groups of 10 each.

[0110] 2. Test area: waist and abdomen.

[0111] 3. Test conditions: The test environment temperature is 20-22℃ and the humidity is 40-60%, and real-time dynamic monitoring is carried out. Before each test, you need to sit quietly in the constant temperature and humidity room for 15 minutes.

[0112] 4. Product Usage: Apply 5-8g of the test sample to the target area (waist and abdomen) twice daily, morning and evening. Massage for 5-10 minutes until absorbed. Also apply to other areas of the body where fat reduction is desired. Use continuously for 4 weeks, conducting tests at weeks 0, 2, and 4.

[0113] 5. Test items and results:

[0114] (1) Three-point positioning method for measuring waist circumference

[0115] Using the lowest point of the volunteer's navel as a fixed point, and then selecting two points at the same level as markers, the waist circumference of the volunteer was measured before and after using the product, respectively, according to the three-point positioning method.

[0116] The criteria for judging the test results are as follows: significant effect, waist circumference reduced by 3cm or more; moderate effect, waist circumference reduced by 1-3cm; no obvious effect, waist circumference reduced by 1cm or less. The test results are shown in Table 4.

[0117] Table 4. Waist circumference test results

[0118]

[0119] (2) Weight changes

[0120] Volunteers' weight was measured before and after product use. The criteria for judging the results were: significant effect (weight loss of 4 catties or more); moderate effect (weight loss of 1-4 catties); and no significant effect (weight loss of 1 catties or less). The test results are shown in Table 5.

[0121] Table 5 Results of Weight Change Test

[0122]

[0123] (3) Self-assessment questionnaire survey

[0124] After the fourth week of testing, questionnaires were distributed to volunteers to evaluate their weight loss effect, skin firmness, and overall rating of the product before and after four weeks of use. The test results are shown in Table 6.

[0125] Table 6 Self-assessment results

[0126]

[0127] in conclusion:

[0128] The test results above show that:

[0129] (1) The slimming composition prepared by the present invention has good mildness and safety in use.

[0130] (2) All embodiments of the present invention can significantly reduce the weight, waist circumference and other indicators of volunteers. Good results can be produced after two weeks of use, and the effect is more significant after four weeks of continuous use. However, each comparative example or control example lacks different effective components, and the final test results are significantly worse than those of the embodiments of the present invention. It can be seen that the various effective components in the slimming composition prepared by the present invention are interconnected and synergistic in mechanism. Each component is indispensable to achieve good slimming effect. They first reduce the entry of lipid molecules into fat cells from the source, then eliminate the inflammation of hypertrophic fat cells and prevent them from growing further. Then, the reed extract containing a large amount of flavonoids and polyphenols is used to activate the decomposition of fat, promote the decomposition of triglycerides and remove excess water from fat cells. Finally, the triple synergistic components of phosphatidylcholine, 1,2-pentanediol and water-soluble azone are used to form liposomes for active substances and at the same time temporarily destroy the structure of lipids in the stratum corneum, thereby enhancing the transport of active substances as a whole and enabling them to reach the subcutaneous fat layer of the human body to the maximum extent to burn fat, thereby exerting the best fat reduction and slimming effect.

[0131] (3) The slimming composition prepared by the present invention has good compatibility and can be added to cosmetics of different systems to exert the effect of fat reduction and slimming.

[0132] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A slimming composition containing reed extract, characterized in that: It is composed of the following components in parts by weight: 1-8 parts of reed extract, 1-5 parts of coffee seed extract, 1-5 parts of ginger extract, 1-10 parts of 1,2-pentanediol, 0.5-3 parts of water-soluble azone, and 0.5-3 parts of phosphatidylcholine; wherein the weight ratio of reed extract, coffee seed extract, and ginger extract is (1-8):(1-5):(1-5), and the weight ratio of 1,2-pentanediol and phosphatidylcholine is (1-10):(0.5-3).

2. The slimming composition containing reed extract according to claim 1, characterized in that: It consists of the following components in parts by weight: 5 parts reed extract, 3 parts coffee seed extract, 3 parts ginger extract, 5 parts 1,2-pentanediol, 2 parts water-soluble azone, and 2 parts phosphatidylcholine.

3. The slimming composition containing reed extract according to claim 1, characterized in that: The preparation steps of the reed extract are as follows: The reed fruit was crushed and dried to obtain reed fruit powder. The reed fruit powder was added to an ethanol aqueous solution and stirred for 30 minutes. Then, it was ultrasonically extracted at 20-40℃ for 1 hour to obtain an extract. The extract was centrifuged for 30 minutes, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain the reed extract.

4. The slimming composition containing reed extract according to claim 3, characterized in that: In the preparation steps of the reed extract: the particle size of the reed fruit powder is 50 mesh; the volume ratio of ethanol to water in the ethanol-water solution is 2:1, and the mass ratio of reed fruit powder to ethanol-water solution is 1:10; the ultrasonic power during ultrasonic extraction is 500W; the centrifugation speed during centrifugation is 3000r / min; and the volume ratio of concentrate to propylene glycol is 1:

5.

5. A slimming composition containing reed extract according to claim 1, characterized in that: The preparation steps of the small-fruit coffee seed extract are as follows: Coffee seeds were crushed and dried to obtain coffee seed powder. The coffee seed powder was added to an acetone aqueous solution and stirred for 30 minutes. Then, it was ultrasonically extracted at 20-40°C for 1 hour to obtain an extract. The extract was centrifuged for 30 minutes, the supernatant was collected and filtered to obtain a filtrate. The filtrate was evaporated and concentrated to obtain a concentrate. Propylene glycol was added to the concentrate, and after stirring evenly, impurities were filtered out to obtain coffee seed extract.

6. The slimming composition containing reed extract according to claim 5, characterized in that: In the preparation steps of the small-fruit coffee seed extract: the particle size of the small-fruit coffee seed powder is 50 mesh; the volume ratio of acetone to water in the acetone aqueous solution is 2:1, and the mass ratio of small-fruit coffee seed powder to propanol aqueous solution is 1:10; the ultrasonic power during ultrasonic extraction is 500W; the centrifugation speed during centrifugation is 3000r / min; and the volume ratio of concentrate to propylene glycol is 1:

5.

7. A slimming composition containing reed extract according to claim 1, characterized in that: The preparation steps of the red ginger extract are as follows: Red ginger root was pulverized and dried to obtain red ginger root powder. The red ginger root powder was added to a supercritical extraction vessel, and carbon dioxide fluid was introduced. Extraction was carried out at 25-35℃ for 2 hours to obtain an extract. The extract was added to a supercritical separation vessel and separated under reduced pressure at 35℃ for 1 hour to obtain a separate. The separate was added to water, and distilled for 2 hours. The steam was then condensed and recovered to obtain red ginger extract.

8. A slimming composition containing reed extract according to claim 7, characterized in that: In the preparation steps of the red ginger extract: the particle size of the red ginger root powder is 50 mesh; the flow rate of carbon dioxide in the supercritical extraction vessel is 10 kg / h, the extraction pressure is 25 MPa; and the pressure in the supercritical separation vessel is 4 MPa.

9. A method for preparing a slimming composition containing reed extract according to any one of claims 1 to 8, characterized in that: Includes the following steps: S1. Mix 1,2-pentanediol and phosphatidylcholine, and stir at 75-80°C until homogeneous to obtain mixture A; S2. Mix coffee seed extract and ginger extract, and stir at 40-45°C until homogeneous to obtain mixture B; S3. Add the reed extract to the mixture A obtained in step S1, and stir at 55-60°C until the mixture is homogeneous to obtain mixture C; S4. Mix the mixture B obtained in step S2 and the mixture C obtained in step S3, homogenize for 4-6 minutes, and then cool to 40°C to obtain mixture D; S5. Add the mixture D obtained in step S4 into a high-pressure homogenizer, turn on the constant temperature water bath to 40°C, and perform three cycles of high-pressure homogenization and emulsification to obtain mixture E. S6. Add the mixture E obtained in step S5 into a microfluidic homogenizer, perform microfluidic homogenization once, cool to room temperature, add water-soluble azone and stir until uniformly mixed to obtain a slimming composition containing reed extract.

10. A method for preparing a slimming composition containing reed extract according to claim 9, characterized in that: In step S4, the homogenization speed is 2500-3500 r / min; in step S5, the pressure of the high-pressure homogenizer is 500-1500 bar; in step S6, the pressure of the microjet homogenizer is 800-1800 bar.

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