Microbial self-repairing agent for recycled concrete cracks
By generating mineralized products on concrete through microbial self-healing agents, the problem of easy erosion of concrete components in marine environments is solved, and good durability and resistance to chloride ions and carbonation are achieved.
Patent Information
- Application Number
- CN202511003149.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-21
- Publication Date
- 2025-11-18
AI Technical Summary
Concrete components in marine environments are susceptible to corrosion and have poor durability. Traditional protection methods are costly, difficult to maintain, and cause environmental pollution.
Microbial self-healing agents are used, including bacterial solution, cementing solution and functional additives. By mixing the bacterial solution and cementing solution, mineral products are generated, forming a dense structure and improving resistance to chloride ion and carbon dioxide erosion.
It effectively resists chloride ion corrosion, has good anti-carbonation properties, reduces carbonation depth, and improves the durability of concrete.
Smart Images
Figure CN120965369A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of concrete self-healing technology, specifically to a microbial self-healing agent for cracks in recycled concrete. Background Technology
[0002] Concrete is widely used due to its advantages such as high compressive strength, good durability, and low cost. However, concrete is a typical brittle material, and under external forces or other factors, it inevitably develops micro-cracks or localized damage, leading to a decrease in its mechanical properties and durability. Concrete components in marine environments, in particular, are subject to various forms of corrosion, such as carbonation corrosion, salt corrosion, and biological corrosion. This causes many concrete structures to prematurely exhibit aggregate stripping of the concrete cover and internal steel reinforcement corrosion before reaching their design lifespan, posing serious safety hazards. Furthermore, marine corrosion also results in economic losses. Traditional protective methods to mitigate these losses include the use of special materials, surface coatings, and corrosion inhibitors. While these methods have some effect, they suffer from high costs, maintenance difficulties, and environmental pollution. Therefore, research is still needed on new microbial self-healing agents for concrete cracks to achieve deeper mineralization and effectively improve durability. Summary of the Invention
[0003] The purpose of this invention is to provide a microbial self-healing agent for cracks in recycled concrete to solve the problems of easy erosion and poor durability of concrete components in existing marine environments.
[0004] To solve the above-mentioned technical problems, the present invention specifically provides the following technical solution: This invention discloses a microbial self-healing agent, comprising a bacterial solution, a cementing solution, and a functional additive; the volume ratio of the bacterial solution to the cementing solution is 1:0.5-1.5.
[0005] Preferably, the bacterial solution is a Bacillus pasteurellii bacterial solution.
[0006] Preferably, the cementing solution is composed of calcium acetate, calcium lactate, urea and water, with the ratio of calcium acetate to water being 1g:5-8ml, the ratio of calcium lactate to water being 1g:40-60ml, and the ratio of urea to water being 1g:15-18ml.
[0007] Preferably, the functional additives include poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid), and the volume ratio of poly(2-hydroxyethyl)-DL-asparagine to the binder is 1g:0.8-1.2L.
[0008] More preferably, the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.3-1. The introduction and use of poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) results in a microbial self-healing agent that effectively captures calcium ions, generates mineralized products on concrete, forms a dense structure, and further enhances resistance to chloride ion and carbon dioxide erosion.
[0009] This invention discloses a method for preparing a microbial self-healing agent, comprising: The bacterial strain was inoculated onto a solid culture medium and allowed to stand for static culture to obtain the 0th generation strain; the 0th generation strain was inoculated into a liquid culture medium and cultured to obtain the working strain; the working strain was then inoculated into a liquid culture medium and cultured to obtain the bacterial solution; the bacterial solution, the cementing solution, and the functional adjuvant were mixed to obtain the microbial self-repair agent; the functional adjuvant included poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid).
[0010] Preferably, the bacterial strain is Bacillus pasteurellii strain, the bacterial solution is Bacillus pasteurellii bacterial solution, and the cementing solution is composed of calcium acetate, calcium lactate, urea and water. The ratio of calcium acetate to water is 1g:5-8ml, the ratio of calcium lactate to water is 1g:40-60ml, the ratio of urea to water is 1g:15-18ml, and the volume ratio of bacterial solution to cementing solution is 1:0.5-1.5.
[0011] Preferably, the volume ratio of poly(2-hydroxyethyl)-DL-asparagine to the cementing liquid is 1g:0.8-1.2L, and the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.3-1.
[0012] This invention also discloses the application of any of the above-mentioned microbial self-healing agents in the preparation of concrete components.
[0013] This invention discloses a method for preparing bacterial culture, comprising: The bacterial strain was inoculated onto a solid culture medium and incubated statically at 27-35℃ for 27-35 hours to obtain the 0th generation strain; the 0th generation strain was inoculated into a liquid culture medium and incubated statically at 27-35℃ for 27-35 hours to obtain the working strain; the working strain was then inoculated into a liquid culture medium and incubated statically at 27-35℃ for 27-35 hours to obtain the bacterial solution.
[0014] Preferably, the bacterial strain is Bacillus pasteurellii.
[0015] Preferably, the obtained bacterial solution is a Bacillus pasteurellii bacterial solution.
[0016] Preferably, the solid culture medium consists of peptone, meat extract, urea, agar, and water.
[0017] More preferably, the ratio of peptone to water is 1g:190-210ml.
[0018] More preferably, the ratio of meat extract to water is 1g:300-400ml.
[0019] More preferably, the ratio of urea to water is 1g:15-55ml.
[0020] More preferably, the ratio of agar to water is 1g:45-55ml.
[0021] Preferably, the liquid culture medium does not use agar compared to the solid culture medium.
[0022] This invention discloses a method for preparing a microbial self-healing agent, comprising: Microbial self-healing agent is obtained by mixing bacterial solution, cementing solution and functional additives.
[0023] Preferably, the bacterial solution is a Bacillus pasteurellium bacterial solution. Preferably, the cementing solution is composed of calcium acetate, urea and water, with the ratio of calcium acetate to water being 1g:5-8ml, the ratio of calcium lactate to water being 1g:40-60ml, and the ratio of urea to water being 1g:15-18ml.
[0024] Preferably, the volume ratio of bacterial solution to cementing solution is 1:0.5-1.5.
[0025] Preferably, the functional additives include poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid).
[0026] More preferably, the volume ratio of poly(2-hydroxyethyl)-DL-asparagine to the cementing liquid is 1g:0.8-1.2L.
[0027] More preferably, the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.3-1.
[0028] This invention discloses a method for preparing isobutylene / MA copolymer, specifically including: Maleic anhydride and ethyl acetate were mixed and heated to 55-70°C under nitrogen and 0.3-0.5 MPa. Then, an initiator solution and isobutylene were added, and the reaction was continued for 2-3 hours. After the reaction was completed, the solid was separated and washed with ethanol and dried to obtain the isobutylene / MA copolymer.
[0029] Preferably, the mass ratio of maleic anhydride to ethyl acetate is 1:1-2.5.
[0030] Preferably, the initiator solution is composed of azobisisobutyronitrile and ethyl acetate, and the mass ratio of azobisisobutyronitrile to ethyl acetate is 1:90-110.
[0031] Preferably, the mass ratio of maleic anhydride to isobutylene is 1:0.2-0.3.
[0032] More preferably, in preparing the microbial self-healing agent, in addition to using poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid), the present invention can also use isobutylene / MA copolymer. The synergistic use of isobutylene / MA copolymer has a synergistic effect, further effectively enhancing the generation of mineralized products by the microbial self-healing agent, improving the pore structure of concrete, and improving the impermeability, thereby improving the chloride ion resistance and carbonation resistance of the repaired concrete, and improving the durability of the concrete.
[0033] Preferably, the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to isobutylene / MA copolymer is 1:0.2-0.9.
[0034] Compared with the prior art, the present invention has the following advantages: This invention proposes a microbial self-healing agent for cracks in recycled concrete. First, a bacterial strain is cultured to obtain a bacterial solution. Then, the bacterial solution, binder, and functional additives are mixed to obtain the microbial self-healing agent. The bacterial strain is *Bacillus pasteurellii*, the binder consists of calcium acetate, calcium lactate, urea, and water, and the functional additives include poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid). The microbial self-healing agent prepared by this invention, after being applied to repair concrete, exhibits good durability, effectively resists chloride ion erosion, and demonstrates good resistance to carbonation with a low carbonation depth. Attached Figure Description
[0035] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.
[0036] Figure 1 The graph shows the determination of resistance to chloride ion corrosion. Figure 2 The graph shows the determination of the carbonization resistance. Detailed Implementation
[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0038] The concepts involved in this application will first be described with reference to the accompanying drawings. It should be noted that the following descriptions of various concepts are only for the purpose of making the content of this application easier to understand and do not constitute a limitation on the scope of protection of this application; furthermore, the embodiments and features in the embodiments of this application can be combined with each other unless otherwise specified. This application will now be described in detail with reference to the accompanying drawings and embodiments.
[0039] Example 1: Preparation of *Pasteurella multocida* bacterial suspension: *Pasteurella multocida* strain was inoculated onto sterilized solid slant agar and incubated statically at 30°C for 24 hours to obtain generation 0. This generation 0 strain was then inoculated into sterilized liquid agar and incubated at 30°C and 150 rpm for 24 hours to obtain working culture. The working culture was then inoculated into sterilized liquid agar and incubated at 30°C and 150 rpm for 24 hours to obtain *Pasteurella multocida* bacterial suspension. The solid agar consisted of peptone, meat extract, urea, agar, and water. The ratio of peptone to water was 1 g:200 ml, the ratio of meat extract to water was 1 g:333 ml, the ratio of urea to water was 1 g:50 ml, and the ratio of agar to water was 1 g:50 ml. The liquid agar was not used in the liquid agar compared to the solid agar. *Pasteurella multocida* preservation number: ATCC-11859.
[0040] Preparation of the microbial self-healing agent: Calcium acetate, calcium lactate, and urea were dissolved in water to obtain a cementing solution. Then, the *Bacillus pasteurellii* bacterial solution and the cementing solution were mixed to obtain the microbial self-healing agent. The ratio of calcium acetate to water was 1g:6.32ml, the ratio of calcium lactate to water was 1g:50ml, the ratio of urea to water was 1g:16.65ml, and the volume ratio of *Bacillus pasteurellii* bacterial solution to the cementing solution was 1:1.
[0041] Example 2: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0042] Preparation of the microbial self-healing agent: Calcium acetate, calcium lactate, and urea were dissolved in water to obtain a cementing solution. Then, the *Bacillus pasteurellii* bacterial solution, the cementing solution, poly(2-hydroxyethyl)-DL-asparagine, and poly(2-acrylamide-2-methylpropanesulfonic acid) were mixed to obtain the microbial self-healing agent. The ratio of calcium acetate to water was 1g:6.32ml, the ratio of calcium lactate to water was 1g:50ml, the ratio of urea to water was 1g:16.65ml, the volume ratio of *Bacillus pasteurellii* bacterial solution to cementing solution was 1:1, the volume ratio of poly(2-hydroxyethyl)-DL-asparagine to cementing solution was 1g:1L, and the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) was 1:0.5.
[0043] Example 3: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0044] Preparation of microbial self-healing agent: The preparation of microbial self-healing agent in this embodiment differs from that in Example 2 in that the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.8, while other conditions and parameters are the same as in Example 2.
[0045] Example 4: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0046] Preparation of microbial self-healing agent: The preparation of microbial self-healing agent in this example differs from that in Example 2 in that the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.35, while other conditions and parameters are the same as in Example 2.
[0047] Example 5: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0048] Preparation of isobutylene / MA copolymer: Maleic anhydride and ethyl acetate were mixed and heated to 60°C under nitrogen atmosphere and 0.4 MPa. Then, an initiator solution and isobutylene were added, and the reaction was continued for 2.5 h. After the reaction was completed, the solid was separated and washed with ethanol and dried to obtain the isobutylene / MA copolymer. The mass ratio of maleic anhydride to ethyl acetate was 1:1.875. The initiator solution consisted of azobisisobutyronitrile and ethyl acetate, with a mass ratio of azobisisobutyronitrile to ethyl acetate of 1:100. The mass ratio of maleic anhydride to isobutylene was 1:0.25.
[0049] Preparation of microbial self-healing agent: Calcium acetate, calcium lactate and urea are dissolved in water to obtain a cementing solution. Then, Bacillus pasteurellosis bacterial solution, cementing solution, poly(2-hydroxyethyl)-DL-asparagine, poly(2-acrylamide-2-methylpropanesulfonic acid) and isobutylene / MA copolymer are mixed to obtain microbial self-healing agent. The ratio of calcium acetate to water is 1g:6.32ml, the ratio of calcium lactate to water is 1g:50ml, the ratio of urea to water is 1g:16.65ml, the volume ratio of Bacillus pasteurellium inoculum to cementing solution is 1:1, the volume ratio of poly(2-hydroxyethyl)-DL-asparagine to cementing solution is 1g:1L, the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.5, and the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to isobutylene / MA copolymer is 1:0.3.
[0050] Example 6: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0051] The preparation of the isobutylene / MA copolymer is the same as in Example 5.
[0052] Preparation of microbial self-healing agent: The preparation of microbial self-healing agent in this example differs from that in Example 5 in that the mass ratio of poly(2-hydroxyethyl)-DL-asparagine to isobutylene / MA copolymer is 1:0.7, and other conditions and parameters are the same as in Example 5.
[0053] Comparative Example 1: The preparation of Pasteurella multocida bacterial suspension is the same as in Example 1.
[0054] Preparation of microbial self-healing agent: The preparation of the microbial self-healing agent in this comparative example differs from that in Example 2 in that poly(2-acrylamide-2-methylpropanesulfonic acid) was not used, while other conditions and parameters were the same as in Example 2.
[0055] Experimental Example 1: For the preparation of concrete specimens, cement, fly ash, and granulated blast furnace slag powder were mixed to obtain a gel material. A mixture of water-reducing agent and water was added to the gel material and stirred for 90 seconds to obtain concrete. The concrete was poured into a mold, vibrated, and leveled. Then, it was covered with plastic film and left to stand at 22°C for 24 hours. The mold was removed and the specimens were cured for 28 days to obtain concrete specimens. The mass ratio of cement to fly ash was 1:0.57, the mass ratio of cement to granulated blast furnace slag powder was 1:0.61, the mass ratio of cement to water-reducing agent was 1:0.02, the mass ratio of gel material to water-reducing agent was 1:0.01, and the mass ratio of gel material to water was 1:0.38.
[0056] Determination of resistance to chloride ion corrosion: Concrete specimens were moistened by spraying water. Microbial self-healing agent was dripped onto the surface of the concrete specimen and spread evenly. After 3 hours, a binder was applied. This process was repeated every 12 hours for 7 consecutive days. Using the fixtures of the electrical flux testing instrument, core samples were drilled from standard cubic concrete specimens and cut into cylindrical specimens with a diameter of 100 mm and a height of 50 mm. After cutting, according to the electrical flux testing specification and the test procedures referring to GB / T50082-2024 "Standard for Test Methods of Long-Term Performance and Durability of Concrete", the sides of the cylinders were evenly coated with epoxy resin and vacuum-sealed for testing.
[0057] The results are as follows Figure 1 As shown, compared to Example 2, Example 1 demonstrates that the use of poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) can reduce the electrical flux, thus improving resistance to chloride ion penetration corrosion. Compared to Example 3, Example 2 demonstrates that increasing the amount of poly(2-acrylamide-2-methylpropanesulfonic acid) within a certain range can also reduce the electrical flux and improve resistance to chloride ion penetration corrosion. Compared to Example 4, Example 2 demonstrates that decreasing the amount of poly(2-acrylamide-2-methylpropanesulfonic acid) within a certain range will increase the electrical flux and decrease resistance to chloride ion penetration corrosion. Compared to Example 5, Example 2 demonstrates that the use of poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) can reduce the electrical flux, thus improving resistance to chloride ion penetration corrosion. Based on poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid), the use of isobutylene / MA copolymer can further reduce the electrical flux and improve the resistance to chloride ion penetration. Compared with Example 6, Example 5 shows that increasing the amount of isobutylene / MA copolymer within a certain range can also reduce the electrical flux and improve the resistance to chloride ion penetration. Compared with Comparative Example 1, Example 2 shows that poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) need to be used in appropriate amounts. Using poly(2-hydroxyethyl)-DL-asparagine alone has no significant effect on reducing the electrical flux and improving the resistance to chloride ion penetration.
[0058] Experimental Example 2: The preparation of concrete specimens is the same as in Experiment 1.
[0059] For the determination of carbonation resistance, concrete specimens were moistened by spraying water. Microbial self-healing agent was then dripped onto the surface of the concrete specimen and spread evenly. After 3 hours, a binder was applied, and the application was repeated every 12 hours for 7 consecutive days. The carbonation resistance test was conducted according to the "Standard for Test Methods of Long-Term Performance and Durability of Concrete" GB / T50082-2024. One side coated with the microbial self-healing slurry was retained, while the other sides were sealed with epoxy resin after removing marine deposits. The specimens were then dried in an oven at 60℃ for 48 hours, followed by carbonation for 7 days at 20℃, 70% humidity, and 20% carbon dioxide. After carbonation, the concrete specimens were cracked and sprayed with phenolphthalein, and the carbonation depth was measured. The application rate of the microbial self-healing agent on the concrete specimen was 0.1 mL / cm². 2 The amount of binder applied to the concrete specimen was 0.1 mL / cm². 2 .
[0060] The results are as follows Figure 2 As shown, compared with Example 2, Example 1 demonstrates that the use of poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) can reduce the carbonization depth, i.e., improve the carbonization resistance. Compared with Example 3, Example 2 demonstrates that increasing the amount of poly(2-acrylamide-2-methylpropanesulfonic acid) within a certain range can also reduce the carbonization depth and improve the carbonization resistance. Compared with Example 4, Example 2 demonstrates that decreasing the amount of poly(2-acrylamide-2-methylpropanesulfonic acid) within a certain range will increase the carbonization depth and reduce the carbonization resistance. Compared with Example 5, Example 2 demonstrates that the use of poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) can reduce the carbonization depth, i.e., improve the carbonization resistance. Based on poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid), the use of isobutylene / MA copolymer can further reduce the carbonization depth and improve the carbonization resistance. Compared with Example 6, Example 5 shows that increasing the amount of isobutylene / MA copolymer within a certain range can also reduce the carbonization depth and improve the carbonization resistance. Compared with Comparative Example 1, Example 2 shows that poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid) need to be used in appropriate amounts. Using poly(2-hydroxyethyl)-DL-asparagine alone has no significant effect on reducing the carbonization depth and improving the carbonization resistance.
[0061] The embodiments and / or implementation methods described above are merely preferred embodiments and / or implementation methods for implementing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any way. Any person skilled in the art can make some modifications or alterations to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as the technology or embodiments that are substantially the same as the present invention.
[0062] This document uses specific examples to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the methods and core ideas of this application. The above descriptions are only preferred embodiments of this application. It should be noted that due to the limitations of written expression, while there are objectively infinite specific structures, those skilled in the art can make several improvements, modifications, or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the inventive concept and technical solution to other situations without modification, should all be considered within the scope of protection of this application.
Claims
1. A microbial self-repairing agent, comprising a bacterial solution, a cementing solution and a functional additive; the volume ratio of the use amount of the bacterial solution to the cementing solution is 1:0.5-1.
5. 2.The microbial self-repairing agent according to claim 1, wherein the bacterial solution is a bacterial solution of Bacillus pasteurii. 3.The microbial self-repairing agent according to claim 1, wherein the cementing solution is composed of calcium acetate, calcium lactate, urea and water; the use amount ratio of calcium acetate to water is 1g:5-8ml; the use amount ratio of calcium lactate to water is 1g:40-60ml; and the use amount ratio of urea to water is 1g:15-18ml. 4.The microbial self-repairing agent according to claim 1, wherein the functional additive comprises poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid); the volume ratio of the use amount of poly(2-hydroxyethyl)-DL-asparagine to the cementing solution is 1g:0.8-1.2L. 5.The microbial self-repairing agent according to claim 4, wherein the mass ratio of the use amount of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.3-1. 6.A preparation method of a microbial self-repairing agent, comprising: inoculating a bacterial strain to a solid culture medium to obtain a 0th generation bacterial strain by static culture; inoculating the 0th generation bacterial strain to a liquid culture medium to obtain a working bacterial solution by culture; inoculating the working bacterial solution to the liquid culture medium to obtain a bacterial solution by culture; and mixing the bacterial solution, a cementing solution, a nucleation additive and a functional additive to obtain the microbial self-repairing agent; the functional additive comprises poly(2-hydroxyethyl)-DL-asparagine and poly(2-acrylamide-2-methylpropanesulfonic acid). 7.The preparation method of the microbial self-repairing agent according to claim 6, wherein the bacterial strain is a bacterial strain of Bacillus pasteurii, the bacterial solution is a bacterial solution of Bacillus pasteurii, the cementing solution is composed of calcium acetate, urea and water; the use amount ratio of calcium acetate to water is 1g:5-8ml; the use amount ratio of urea to water is 1g:15-18ml; and the volume ratio of the use amount of the bacterial solution to the cementing solution is 1:0.5-1.
5. 8.The preparation method of the microbial self-repairing agent according to claim 6, wherein the nucleation additive comprises dimercaprol, diethylenetriamine pentaacetic acid and polydiallyldimethylammonium chloride; the use amount ratio of dimercaprol to the bacterial solution of Bacillus pasteurii is 1g:4-6L; the mass ratio of the use amount of dimercaprol to diethylenetriamine pentaacetic acid is 1:0.5-1.7; and the mass ratio of the use amount of dimercaprol to polydiallyldimethylammonium chloride is 1:0.15-1. 9.The preparation method of the microbial self-repairing agent according to claim 6, wherein the volume ratio of the use amount of poly(2-hydroxyethyl)-DL-asparagine to the cementing solution is 1g:0.8-1.2L; and the mass ratio of the use amount of poly(2-hydroxyethyl)-DL-asparagine to poly(2-acrylamide-2-methylpropanesulfonic acid) is 1:0.3-1. 10.The microbial self-repairing agent according to any one of claims 1-5, used in the preparation of a concrete component.