Integrated extraction device for free nucleic acid in biological fluid based on electromagnetic magnetic bar

Abstract

The invention relates to an electromagnetic bar-based biological fluid free nucleic acid integrated extraction device, which comprises a box body, a box door is rotatably connected to the box body, a driving assembly is arranged in the box body, a sliding block is arranged on the driving assembly, an ionization magnetic bar is fixedly arranged in the sliding block, and the ionization magnetic bar is arranged on the box door. A mixing tank, a cleaning tank I, a cleaning tank II, an elution tank and a collecting pipe are sequentially arranged on the bottom surface in the box body, a communicating pipeline is arranged between the elution tank and the collecting pipe, and the ionization magnetic bar magnetically adsorbs magnetic beads; the ionization magnetic bar moves in the box body, and manual operation is performed on the wall surface, so that the number of magnetic beads is reduced, personal errors are reduced, and operation is simple.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biomedical detection, and particularly relates to a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod. BACKGROUND

[0002] As a non-invasive biomarker, urine free nucleic acid is increasingly important in clinical diagnosis, but there are some technical problems at present. Low-concentration nucleic acid capture difficulty: the concentration of urine nucleic acid is low (ng / mL level), the combination efficiency of the traditional filter membrane method is insufficient, resulting in low recovery rate (<50%); Serious matrix interference: high salt, urea, metabolites and other interference magnetic bead-nucleic acid combination, resulting in false negative downstream detection; Serious loss of magnetic bead transfer: repeated magnetic bead transfer (mixing-> washing-> elution) in manual operation causes serious loss of magnetic beads; Complex operation and time-consuming: repeated pipetting, separation of magnetic beads, single sample extraction takes a long time; Poor batch stability: manual operation difference leads to large fluctuation of recovery rate. SUMMARY

[0003] In view of the above problems in the prior art, the main purpose of the present application is to provide a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod.

[0004] The technical scheme of the present application is as follows: a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod, comprising a box body, a box door is rotatably connected to the box body, a driving assembly is arranged inside the box body, a sliding block is arranged on the driving assembly, an ionization magnetic rod is fixedly arranged in the sliding block, a mixing tank, a washing tank one, a washing tank two, an elution tank and a collection pipe are sequentially arranged on the bottom surface of the box body, a communication pipeline is arranged between the elution tank and the collection pipe, and the ionization magnetic rod magnetically attracts magnetic beads.

[0005] As a preferred embodiment, the driving assembly comprises a servo motor, the output end of the servo motor is fixedly connected with a threaded shaft, the threaded shaft penetrates through the sliding block, a limiting rod is arranged on one side of the threaded shaft, the limiting rod is fixedly connected with the box body, the limiting rod penetrates through the sliding block, and the end of the threaded shaft away from the servo motor is rotatably connected with the box body.

[0006] As a preferred embodiment, the ionization magnetic rod is opposite to the mixing tank below, and the mixing tank, the washing tank one, the washing tank two, the elution tank and the collection pipe are fixed in a horizontal straight line.

[0007] As a preferred embodiment, the alcohol solution containing lyotropic salt is filled in the washing tank one, and the low ionic strength buffer is filled in the washing tank two.

[0008] As a preferred implementation, the connecting pipeline is provided with a magnetic bead filter membrane.

[0009] Compared with the prior art, the application has the advantages and positive effects that the ionization magnetic rod moves in the box body, the wall surface is manually operated, the number of magnetic beads is reduced, human error is reduced, and the operation is simple. BRIEF DESCRIPTION OF DRAWINGS

[0010] Figure 1 The application provides a whole structure schematic diagram of a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod; Figure 2 The application provides a sample and combined liquid mixed device state schematic diagram of a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod; Figure 3 The application provides an elution device state schematic diagram of a biological fluid free nucleic acid integrated extraction device based on an electromagnetic magnetic rod.

[0011] Legend: 1, box body; 2, box door; 3, driving assembly; 4, sliding block; 5, ionization magnetic rod; 6, mixing tank; 7, washing tank one; 8, cleaning tank two; 9, elution tank; 10, collection pipe; 11, connecting pipeline. DETAILED DESCRIPTION

[0012] In order to make the purpose, technical scheme and advantages of the embodiments of the application clearer, the technical scheme in the embodiments of the application will be described clearly and completely below in conjunction with the drawings in the embodiments of the application. Obviously, the described embodiments are some embodiments of the application, rather than all the embodiments. The components of the embodiments of the application described and shown in the drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the application provided in the drawings is not intended to limit the scope of the claimed application, but only represents selected embodiments of the application. All other embodiments obtained by those of ordinary skill in the art without creative work on the basis of the embodiments in the application belong to the scope of protection of the application.

[0013] In the description of the application, it should be noted that the orientations or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the application. In addition, the terms "first", "second", "third" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0014] In the description of the present application, it is also necessary to explain that, unless otherwise explicitly specified and limited, the terms "arrangement", "installation", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0015] The present application will be further described below with reference to the accompanying drawings and specific embodiments Embodiments The biological fluid free nucleic acid integrated extraction device based on electromagnetic magnet bar comprises a box body 1, a box door 2 is rotatably connected on the box body 1, a driving assembly 3 is arranged inside the box body 1, a sliding block 4 is arranged on the driving assembly 3, an ionization magnet bar 5 is fixedly arranged in the sliding block 4, a mixing tank 6, a washing tank 7, a washing tank 8, an elution tank 9 and a collection pipe 10 are sequentially arranged on the bottom surface in the box body 1, a communication pipeline 11 is arranged between the elution tank 9 and the collection pipe 10, the ionization magnet bar 5 magnetically attracts magnetic beads, the ionization magnet bar 5 is moved by the driving assembly 3, so that the ionization magnet bar 5 sequentially enters the mixing tank 6, the washing tank 7, the washing tank 8, the elution tank 9 and the collection pipe 10, and the transfer of the magnetic beads is not required, thereby realizing the error of human operation and human error.

[0016] The driving assembly 3 comprises a servo motor, the output end of the servo motor is fixedly connected with a threaded shaft, the threaded shaft penetrates through the sliding block 4, a limiting rod is arranged on one side of the threaded shaft, the limiting rod is fixedly connected with the box body 1, the limiting rod penetrates through the sliding block 4, and the end of the threaded shaft away from the servo motor is rotatably connected with the box body 1. The servo motor is reversely rotated to drive the sliding block 4 to move, and the moving direction of the sliding block 4 is limited by the limiting rod.

[0017] The ionization magnet bar 5 is opposite to the mixing tank 6 below, the mixing tank 6, the washing tank 7, the washing tank 8, the elution tank 9 and the collection pipe 10 are fixed in a horizontal straight line, and the ionization magnet bar 5 sequentially enters the corresponding container.

[0018] An alcohol solution containing a chaotropic salt is poured into the washing tank 7, and a low ionic strength buffer is poured into the washing tank 8, the washing tank 7 removes salt and hydrophobic impurities, and the washing tank 8 removes residual organic solvents.

[0019] The communication pipeline 11 is provided with a magnetic bead filter membrane for filtering liquid, and is provided with a solenoid valve.

[0020] Working principle: As shown in the figure, 1. combination: the urine sample is injected into the mixing tank 6, and then the ionization magnetic bar 5 is powered to sink, at this time the magnetic beads combine the nucleic acid under the electric field; 2. Cleaning 1: through the ionization magnetic bar 5 to adsorb the magnetic beads, start the servo motor, drive the ionization magnetic bar 5 to move to the cleaning tank 1 7 above, the ionization magnetic bar 5 is powered to sink, the magnetic beads are suspended by closing the electric field, and the impurities are removed; 3. Cleaning 2: through the ionization magnetic bar 5 to adsorb the magnetic beads, start the servo motor, drive the ionization magnetic bar 5 to move to the cleaning tank 2 8, the ionization magnetic bar 5 is powered to sink, the magnetic beads are suspended by closing the electric field, and the organic solvent residue is removed; 4. Elution: through the ionization magnetic bar 5 to adsorb the magnetic beads, start the servo motor, drive the ionization magnetic bar 5 to move to the elution tank 9 above, the magnetic beads are located in the elution tank 9, and the ionization magnetic bar 5 is used to apply an alternating electric field to weaken the electrostatic attraction and make the nucleic acid dissociate efficiently; 5. Collection: through the ionization magnetic bar 5 to adsorb the magnetic beads, open the electromagnetic valve, the liquid passes through the magnetic bead filter membrane, and is stored in the collection tube 10.

[0021] Finally, it should be pointed out that: the above-described embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. An integrated extraction device for free nucleic acids from biological body fluids based on electromagnetic rods, comprising a housing (1), characterized in that: The box body (1) is rotatably connected to the box door (2). The box body (1) is provided with a drive assembly (3). The drive assembly (3) is provided with a slider (4). An ionizing magnetic rod (5) is fixedly provided inside the slider (4). The bottom surface inside the box body (1) is provided with a mixing tank (6), a first cleaning tank (7), a second cleaning tank (8), an elution tank (9), and a collection tube (10) in sequence. A connecting pipe (11) is provided between the elution tank (9) and the collection tube (10). The ionizing magnetic rod (5) magnetically adsorbs magnetic beads.

2. The integrated extraction device for free nucleic acids in biological body fluids based on an electromagnetic rod according to claim 1, characterized in that: The drive assembly (3) includes a servo motor, the output end of which is fixedly connected to a threaded shaft, the threaded shaft passing through a slider (4), a limiting rod on one side of the threaded shaft, the limiting rod being fixedly connected to a housing (1), the limiting rod passing through the slider (4), and the end of the threaded shaft away from the servo motor being rotatably connected to the housing (1).

3. The integrated extraction device for free nucleic acids in biological body fluids based on an electromagnetic rod according to claim 1, characterized in that: The ionizing magnetic rod (5) is directly below the mixing tank (6), and the mixing tank (6), the first cleaning tank (7), the second cleaning tank (8), the elution tank (9) and the collection tube (10) are fixed in a horizontal straight line.

4. The integrated extraction device for free nucleic acids in biological body fluids based on an electromagnetic rod according to claim 1, characterized in that: The first cleaning tank (7) is filled with an alcohol solution containing ionized salt, and the second cleaning tank (8) is filled with a low ionic strength buffer solution.

5. The integrated extraction device for free nucleic acids in biological body fluids based on an electromagnetic rod according to claim 1, characterized in that: The connecting pipe (11) is equipped with a magnetic bead filter membrane.

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