Full-automatic intelligent thin-layer chromatographic analyzer

Abstract

The invention discloses a full-automatic intelligent thin-layer chromatographic analyzer, and relates to the field of chromatographic detection equipment. The analyzer comprises a base, wherein a thin-layer plate moving mechanism, a thin-layer plate storage mechanism, a sample application mechanism, a display plate mechanism and an image acquisition mechanism are arranged on the base. And the thin-layer plate moving mechanism can drive the thin-layer plate to sequentially pass through the sample application mechanism, the plate display mechanism and the image acquisition mechanism from the thin-layer plate storage mechanism. Wherein the thin-layer plate moving mechanism comprises an X-axis moving part, a supporting frame body, a first electromagnetic valve, a second electromagnetic valve and a supporting guide rod; the thin-layer plate storage mechanism comprises a plate bin; the sample application mechanism comprises a liquid storage assembly and a sample application assembly; the display board mechanism comprises a display board assembly and a liquid injection assembly; and the image acquisition mechanism comprises a camera obscura, a bearing platform, a second camera and an ultraviolet lamp. Automatic operation of the whole process of the thin-layer chromatography experiment is achieved, the detection efficiency and the accuracy and repeatability of the result are improved, and the labor intensity and health and safety risks of experimenters are reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of chromatographic detection equipment, and particularly relates to a full-automatic intelligent thin-layer chromatography analyzer. BACKGROUND

[0002] As a common experimental technology for rapid separation, qualitative or quantitative analysis of a small amount of substances, thin-layer chromatography is widely used in the fields of drug component identification, sample impurity inspection, content determination and reaction progress tracking. The traditional experimental process mainly includes the following steps: uniformly coating a suitable stationary phase on a carrier to form a thin-layer chromatography plate; using a capillary to dip a small amount of sample solution to be tested to a suitable position of the thin-layer plate; immersing one end of the thin-layer plate into a developing cylinder containing a developing agent (the sample application position is not submerged); taking out and drying when the front of the developing agent does not reach the other end of the thin-layer plate to obtain a thin-layer chromatography result graph; and finally performing qualitative and rough quantitative analysis by comparing the Rf value and observing the color depth of the color spot.

[0003] However, the thin-layer chromatography experiment of most laboratories at the present stage still relies on manual operation, and there are many problems: Sample application link: manual sample application cannot accurately control the amount of solution dipped by the capillary, too much sampling will cause tailing of the chromatogram, poor separation effect, and insufficient sampling will make the spot indistinct and difficult to identify, reducing the detection accuracy; mechanical repetitive operation easily makes the experimenters tired, leading to operation deviation and unable to guarantee the repeatability of the results; and when detecting toxic and harmful samples, it also brings health and safety risks to the experimenters.

[0004] Plate development link: the process is complicated, and the experimenters need to pay attention to the position of the front of the developing agent at all times to prevent it from exceeding the thin-layer plate and being unable to calculate the key Rf value data.

[0005] Data acquisition link: the sample application position and the front position of the developing agent need to be manually marked, and the center moving distance of the spot is measured manually after drying; even if a camera is used for acquisition, the thin-layer plate also needs to be manually placed at a specified position of a dark box, which is inconvenient to operate and is easy to introduce errors.

[0006] In addition, some existing related equipment has defects in thin-layer plate transfer, consumable replenishment, equipment integration and safety, such as inaccurate positioning due to gravity sliding transfer, affecting efficiency due to the need to stop for consumable replenishment, large equipment volume or lack of safety protection, etc. SUMMARY

[0007] The present application mainly aims at the problems of low accuracy, poor repeatability, low efficiency, high labor intensity and safety risks in the manual operation of the thin-layer chromatography experiment in the prior art, and provides a full-automatic intelligent thin-layer chromatography analyzer, which realizes the automation of the whole process of the thin-layer chromatography experiment, improves the detection efficiency and result quality, and guarantees the safety of the experimenters.

[0008] The object of the present application is mainly achieved by the following scheme: A full-automatic intelligent thin layer chromatography analyzer, comprising a base, a thin layer plate moving mechanism, a thin layer plate storage mechanism, a sample application mechanism, a plate unfolding mechanism and an image acquisition mechanism are arranged on the base, the thin layer plate moving mechanism can drive the thin layer plate to pass through the sample application mechanism, the plate unfolding mechanism and the image acquisition mechanism in sequence from the thin layer plate storage mechanism; The thin layer plate moving mechanism comprises an X-axis moving piece, a support frame body, a first electromagnetic valve, a second electromagnetic valve and a support guide rod, the support frame body is connected with the X-axis moving piece, and the X-axis moving piece can drive the support frame body to move along the X-axis direction, the first electromagnetic valve and the second electromagnetic valve are installed on the top of the support frame body along the X-axis direction, and the first electromagnetic valve and the second electromagnetic valve can both drive the thin layer plate to move along the support guide rod, and the support guide rod is arranged along the X-axis direction; The thin layer plate storage mechanism comprises a plate bin, the plate bin is installed on the upper side of the X-axis moving piece, the rear end of the thin layer plate is inserted into the clamp, and a vertical channel for placing the thin layer plate and the clamp is arranged in the plate bin, and the lowermost thin layer plate and the clamp are supported by the support guide rod; The sample application mechanism comprises a liquid storage assembly and a sample application assembly; the liquid storage assembly comprises a first Z-axis moving piece, a disc, a first Schott bottle for storing rinsing liquid, a second Schott bottle for storing waste liquid, a third Schott bottle for storing liquid to be detected and a cotton ball, the first Z-axis moving piece can drive the disc to rotate, the first Schott bottle, the second Schott bottle, the third Schott bottle and the cotton ball are all installed at the edge of the upper surface of the disc and are uniformly distributed along the axis of the disc, and a fan-shaped through hole is formed in the disc; the sample application assembly comprises a second Z-axis moving piece, a third Z-axis moving piece and a sample injector, the second Z-axis moving piece can drive the third Z-axis moving piece to move up and down along the Z-axis direction, and the third Z-axis moving piece can drive the sample application needle push rod of the sample injector to move up and down along the Z-axis direction; The plate unfolding mechanism comprises a plate unfolding assembly and a liquid injection assembly, the plate unfolding assembly comprises an unfolding cylinder and a Y-axis moving piece, the Y-axis moving piece can drive the unfolding cylinder to move back and forth along the Y-axis direction, the liquid injection assembly comprises a magnetic stirrer, an unfolding agent bottle, a peristaltic pump, a filling module and a luer joint, the luer joint is installed on the filling module, and the peristaltic pump can inject the unfolding agent in the unfolding agent bottle into the unfolding cylinder through the luer joint; The image acquisition mechanism comprises a dark box, a bearing platform, a second camera and an ultraviolet lamp, the dark box is covered on the outside of the bearing platform, and the second camera and the ultraviolet lamp are both installed on the top of the dark box.

[0009] Preferably, the side wall of the clamp is integrally provided with a limiting sliding block, a limiting sliding groove matched with the limiting sliding block is vertically arranged on the inner wall of the plate bin, and a clamping groove is formed in the bottom of the clamp along the X-axis direction.

[0010] Preferably, there are two support guide rods, one end of each support guide rod is connected to the bottom of the plate compartment, and the other end of the support guide rod is installed on the side wall of the dark box. The rear support guide rod is located in the slot at the bottom of the clamp. The clamp can move along the rear support guide rod through the slot. The bottom of the thin plate rests on the front support guide rod, and the front support guide rod has a second notch corresponding to the position of the unfolding cylinder. The bottom of the plate compartment has an opening on the side facing the sampling mechanism for the bottom thin plate and the clamp to pass through.

[0011] Preferably, the fixture has a slot on its rear side, into which the first and second solenoid valves can be inserted and move the fixture and the thin plate along the X-axis. A second baffle is installed at the bottom of the support frame, and a second photoelectric switch that works with the second baffle is installed on the base. When the second photoelectric switch detects the second baffle, the first solenoid valve near the thin plate storage mechanism corresponds to the slot of the lowest fixture.

[0012] Preferably, a third baffle is installed at the bottom of the disk near its center, and a third photoelectric switch that works in conjunction with the third baffle is installed on the side wall of the first Z-axis moving part.

[0013] Preferably, a first micro switch is installed on the base directly below the injector, and a first notch is provided on the support guide rod for installing the first micro switch.

[0014] Preferably, a first servo motor is installed on the front side of the deployment cylinder, and a cylinder cover is fastened to the top of the deployment cylinder. The output end of the first servo motor is connected to the front side of the cylinder cover, and the first servo motor can drive the cylinder cover to rotate. A third micro switch is provided on the base in front of the Y-axis moving part.

[0015] Preferably, a second servo motor is provided on one side of the deployment cylinder on the base. The output end of the second servo motor is connected to the refueling module and can drive the refueling module to rotate. A vibration motor is installed on the refueling module. A first heating fan facing the Luer joint is also installed on the base. A second heating fan and a first camera are installed on the base above the deployment cylinder.

[0016] Preferably, a micro pump is installed on the lower surface of the base, the inlet of the micro pump is connected to the bottom of the expansion cylinder through a pipe, a waste expansion agent collection box is inserted on the front side of the base corresponding to the position of the expansion cylinder, and a liquid outlet is also provided on the front side of the expansion cylinder.

[0017] Preferably, the dark box has an outlet and an inlet on its left and right sides, respectively, and a fourth servo motor and a third servo motor are mounted on the front end of the support platform via a bracket. The output end of the fourth servo motor is connected to an outlet door that can block the outlet, and the output end of the third servo motor is connected to an inlet door that can block the inlet. A notch for supporting the clamp is provided on the rear side of the upper surface of the support platform.

[0018] In summary, compared with the prior art, the present invention has the following beneficial technical effects: (1) The present invention uses a thin-layer plate moving mechanism to drive the thin-layer plate through the spotting, spreading and image acquisition mechanisms in sequence, realizing a fully automated operation from thin-layer plate removal, spotting, spreading to result acquisition and analysis, without manual intervention, which greatly improves experimental efficiency; (2) The present invention improves the accuracy and repeatability of detection. The sampling mechanism can accurately control the sampling amount, avoiding the problems of tailing or blurry spots caused by inaccurate sampling amount when manually sampling. The display plate mechanism can accurately control the development process, ensuring the accuracy of the front position of the developing agent and ensuring the reliability of the ratio shift calculation. The image acquisition mechanism avoids the errors caused by manual marking and measurement, making the detection results more accurate. Moreover, the operation of each step is standardized, improving the repeatability of the results. (3) This invention ensures the safety of laboratory personnel. For the detection of toxic and harmful samples, the automated operation reduces the direct contact between laboratory personnel and samples, thereby reducing health and safety risks. (4) The consumables of the present invention are easy to replenish. The developing liquid, thin film and other consumables can be replenished without stopping the machine, which improves the operating efficiency of the equipment; (5) The present invention is highly integrated and safe. The device has a closed body and an isolated internal space. It can be connected to a ventilation device to discharge organic vapors. The casing covers all moving parts, eliminating the risk of accidental injury to the user. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural schematic diagram of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 2 This is a front view of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 3 This is a schematic diagram of the internal three-dimensional structure of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 4 This is an internal front view of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 5 This is an internal top view of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 6 This is an internal side view of a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 7 This is a schematic diagram of the thin-layer plate moving mechanism in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 8 This is a schematic diagram of the thin-layer plate storage mechanism in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 9 This is a schematic diagram of the fixture in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 10 This is a schematic diagram of the sample spotting mechanism in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 11 This is a schematic diagram of the display panel assembly in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 12 This is a schematic diagram of the structure of a fully automatic intelligent thin-layer chromatography analyzer according to the present invention, showing the thin-layer plate sliding into the developing cylinder; Figure 13 This is a schematic diagram of the image acquisition mechanism in a fully automated intelligent thin-layer chromatography analyzer according to the present invention; Figure 14 This is an exploded schematic diagram of the image acquisition mechanism of a fully automated intelligent thin-layer chromatography analyzer according to the present invention.

[0020] Reference numerals: 1-Base; 2-Thin-layer plate moving mechanism; 3-Thin-layer plate storage mechanism; 4-Sampling mechanism; 5-Display plate mechanism; 6-Image acquisition mechanism; 7-Thin-layer plate; 8-X-axis moving component; 9-Support frame; 10-First solenoid valve; 11-Second solenoid valve; 12-Support guide rod; 13-Plate hopper; 14-First Z-axis moving component; 15-Disc; 16-First vial; 17-Second vial; 18-Third vial; 19-Cotton ball; 20-Fan-shaped through-hole; 21-Second Z-axis moving component; 22-Third Z-axis moving component; 23-Injector; 24-Sampling needle pusher; 25-Developing cylinder; 26-Y-axis moving part; 27-Magnetic stirrer; 28-Developing solvent bottle; 29-Peristaltic pump; 30-Dispensing module; 31-Luer connector; 32-Dark box; 33-Bearing platform; 34-Second camera; 35-Ultraviolet lamp; 36-Limiting groove; 37-Limiting slider; 38-Slot; 39-Second notch; 40-Slot; 41-Second baffle; 42-Second photoelectric switch; 43-Third baffle; 44-Third photoelectric switch; 45-First micro switch; 46-First notch; 47-First servo motor; 48-Cylinder head; 49-Third micro switch; 50-Second servo motor; 51-Vibration motor; 52-First heating fan; 53-Second heating fan; 54-First camera; 55-Miniature pump; 56-Waste solvent collection box; 57-Outlet; 58-Fourth servo motor; 59-Third servo motor; 60-Outlet door; 61-Inlet door; 62-Notch; 63-Clamp; 64-Housing; 65-Alarm; 66-Transverse slide rail; 67-X-axis motor; 68-First drive belt; 69-First drive pulley; 70-First driven pulley; 71-First slider ; 72-First Z-axis motor; 73-First vertical support; 74-First vertical slide rail; 75-Second slider; 76-Second Z-axis motor; 77-First threaded rod; 78-First photoelectric switch; 79-First baffle; 80-Second vertical support; 81-Second vertical slide rail; 82-Third slider; 83-Third Z-axis motor; 84-Second threaded rod; 85-Pressure block; 86-Second micro switch; 87-Y-axis motor; 88-Longitudinal slide rail; 89-Fourth slider; 90-Second transmission belt; 91-Second driving pulley; 92-Second driven pulley. Detailed Implementation

[0021] The technical solution of the present invention will be further described in detail below through specific embodiments and in conjunction with the accompanying drawings. It should be understood that the implementation of the present invention is not limited to the following embodiments, and any modifications and / or alterations made to the present invention will fall within the protection scope of the present invention.

[0022] Example 1: like Figures 1-6As shown, this invention discloses a technical solution: a fully automatic intelligent thin-layer chromatography analyzer, including a base 1. The base 1 is equipped with a thin-layer plate moving mechanism 2, a thin-layer plate storage mechanism 3, a sample spotting mechanism 4, a display plate mechanism 5, and an image acquisition mechanism 6. The thin-layer plate moving mechanism 2 can drive the thin-layer plate 7 from the thin-layer plate storage mechanism 3 through the sample spotting mechanism 4, the display plate mechanism 5, and the image acquisition mechanism 6 in sequence. The base 1 is welded or bolted with a frame for mounting the above-mentioned mechanisms and a housing 64 that encloses all moving parts. The housing 64 is equipped with multiple ventilation devices for discharging organic vapors. The ventilation devices are axial flow fans, and an alarm 65 is also installed on the housing.

[0023] Specifically, the thin-layer plate moving mechanism 2 includes an X-axis moving component 8, a support frame 9, a first solenoid valve 10, a second solenoid valve 11, and a support guide rod 12. The support frame 9 is connected to the X-axis moving component 8, and the X-axis moving component 8 can drive the support frame 9 to move along the X-axis direction. The first solenoid valve 10 and the second solenoid valve 11 are installed on the top of the support frame 9 along the X-axis direction, and both the first solenoid valve 10 and the second solenoid valve 11 can drive the thin-layer plate 7 to move along the support guide rod 12. The support guide rod 12 is set along the X-axis direction.

[0024] like Figure 7 As shown, this embodiment provides a specific structure of the X-axis moving component 8: the X-axis moving component 8 includes a transverse slide rail 66, an X-axis motor 67, a first transmission belt 68, a first driving pulley 69, and a first driven pulley 70. The X-axis motor 67 is fixedly installed on the base 1 on one side of the image acquisition mechanism 6 by bolts. The output end of the X-axis motor 67 is fixedly sleeved with the first driving pulley 69. The first driven pulley 70 is rotatably connected to one side of the thin-layer plate storage mechanism 3 on the base 1. The first driving pulley 69 and the first driven pulley 70 are connected by the first transmission belt 68. The bottom of the support frame 9 is equipped with a first slider 71 that is adapted to the transverse slide rail 66. The first slider 71 is slidably connected to the transverse slide rail 66, and the first transmission belt 68 is detachably fixed to the bottom of the support frame 9 by bolts. The transverse slide rail 66 adopts a slide rail with a T-shaped structure at the top. The bottom of the first slider 71 is provided with a groove that is adapted to the shape of the upper part of the transverse slide rail 66.

[0025] Specifically, the thin-layer plate storage mechanism 3 includes a plate hopper 13 bolted to the upper surface of the base 1. The plate hopper 13 is mounted above one side of the X-axis moving part 8. The rear end of the thin-layer plate 7 is inserted into the clamp 63. The plate hopper 13 is provided with a vertical channel for placing the thin-layer plate 7 and the clamp 63. The bottom thin-layer plate 7 and the clamp 63 are supported by the support guide rod 12.

[0026] Specifically, the sampling mechanism 4 includes a liquid storage component and a sampling component; the liquid storage component includes a first Z-axis moving part 14, a disk 15, a first vial 16 for storing washing solution, a second vial 17 for storing waste liquid, a third vial 18 for storing the liquid to be tested, and a cotton ball 19. The first Z-axis moving part 14 can drive the disk 15 to rotate. The first vial 16, the second vial 17, the third vial 18, and the cotton ball 19 are all installed at the edge of the upper surface of the disk 15 and are evenly distributed circumferentially along the axis of the disk 15. A fan-shaped through hole 20 is opened on the disk 15; the sampling component includes a second Z-axis moving part 21, a third Z-axis moving part 22, and an injector 23. The second Z-axis moving part 21 can drive the third Z-axis moving part 22 to move up and down along the Z-axis direction. The third Z-axis moving part 22 can drive the sampling needle push rod 24 of the injector 23 to move up and down along the Z-axis direction.

[0027] This embodiment provides a specific structure of the first Z-axis moving part 14: the first Z-axis moving part 14 includes a first Z-axis motor 72, which is fixedly mounted on the base 1 by bolts.

[0028] This embodiment provides a specific structure of the second Z-axis moving component 21: the second Z-axis moving component 21 includes a first vertical support 73, a first vertical slide rail 74, a second slider 75, a second Z-axis motor 76, and a first threaded rod 77. The bottom of the first vertical support 73 is fixedly installed on the base 1 by bolts. The first vertical slide rail 74 is fixedly installed on the front side of the first vertical support 73, and the second slider 75 is slidably connected to the first vertical slide rail 74. The upper and lower ends of the first threaded rod 77 are rotatably connected to the upper and lower ends of the first vertical support 73, and the first threaded rod 77 and the second slider 75 are threadedly connected. The second Z-axis motor 76 is connected to the first threaded rod 77 through belt drive or gear drive. A first photoelectric switch 78 is installed on the side wall of the first vertical slide rail 74, and a first baffle 79 that cooperates with the first photoelectric switch 78 is installed on the side wall of the second slider 75. The structure of the first vertical slide rail 74 and the second slider 75 is similar to that of the horizontal slide rail 66 and the first slider 71.

[0029] This embodiment provides a specific structure of the third Z-axis moving component 22: the third Z-axis moving component 22 includes a second vertical support 80, a second vertical slide rail 81, a third slider 82, a third Z-axis motor 83, and a second threaded rod 84. The second vertical support 80 is fixedly connected to the front side of the second slider 75, the second vertical slide rail 81 is fixedly installed on the front side of the second vertical support 80, the third slider 82 is slidably connected to the second vertical slide rail 81, the two ends of the second threaded rod 84 are rotatably connected to the upper and lower ends of the second vertical support 80, the top of the second vertical slide rail 81 is equipped with the third Z-axis motor 83 which is connected to the second threaded rod 84, the side wall of the injector 23 is fixedly installed on the lower part of the second vertical slide rail 81, the upper side of the third slider 82 is equipped with a pressure block 85, the inside of the pressure block 85 is provided with a limiting groove, the top of the sampling needle push rod 24 is locked in the limiting groove, and the upper part of the second vertical slide rail 81 is equipped with a second micro switch 86.

[0030] Specifically, the display panel mechanism 5 includes a display panel assembly and a liquid injection assembly. The display panel assembly includes a development cylinder 25 and a Y-axis moving part 26. The Y-axis moving part 26 can drive the development cylinder 25 to move back and forth along the Y-axis direction. The liquid injection assembly includes a magnetic stirrer 27, a developing agent bottle 28, a peristaltic pump 29, a dispensing module 30, and a Luer connector 31. The Luer connector 31 is installed on the dispensing module 30. The peristaltic pump 29 can inject the developing agent in the developing agent bottle 28 into the development cylinder 25 through the Luer connector 31.

[0031] This embodiment provides a specific structure for the Y-axis moving component 26: the Y-axis moving component 26 includes a Y-axis motor 87, a longitudinal slide rail 88, a fourth slider 89, a second transmission belt 90, a second driving pulley 91, and a second driven pulley 92. The fourth slider 89 is installed at the bottom of the unfolding cylinder 25 and is slidably connected to the longitudinal slide rail 88. The longitudinal slide rail 88 is longitudinally installed on the base 1. The Y-axis motor 87 is fixedly installed on the base, and the output end of the Y-axis motor 87 is fixedly sleeved with the second driving pulley 91. The second driven pulley 92 is rotatably connected to the base 1. The second driving pulley 91 and the second driven pulley 92 are connected by the second transmission belt 90, and the second transmission belt 90 is detachably fixed to the bottom of the unfolding cylinder 25 by bolts.

[0032] Specifically, the image acquisition mechanism 6 includes a dark box 32, a support platform 33, a second camera 34, and an ultraviolet lamp 35. The dark box 32 is covered on the outside of the support platform 33, and the second camera 34 and the ultraviolet lamp 35 are both installed on the top of the dark box 32.

[0033] Example 2: like Figure 8 , 9As shown, the present invention discloses another technical solution, a fully automatic intelligent thin-layer chromatography analyzer, which differs from Embodiment 1 in that, specifically, the side wall of the clamp 63 is integrally provided with a limiting slider 37, the inner wall of the plate compartment 13 is vertically provided with a limiting groove 36 that cooperates with the limiting slider 37, and the bottom of the clamp 63 is provided with a slot 38 along the X-axis direction.

[0034] Specifically, there are two support guide rods 12, one end of each support guide rod 12 is fixedly connected to the bottom of the plate compartment 13, and the other end of the support guide rod 12 is fixedly installed on the side wall of the dark box 32. The rear support guide rod 12 is located in the slot 38 at the bottom of the clamp 63. The clamp 63 can move along the rear support guide rod 12 through the slot 38. The bottom of the thin plate 7 is placed on the front support guide rod 12. The front support guide rod 12 has a second notch 39 corresponding to the position of the unfolding cylinder 25, which facilitates the forward and backward movement of the unfolding cylinder 25. The bottom of the plate compartment 13 has an opening on the side facing the sample dispensing mechanism 4 for the bottom thin plate 7 and the clamp 63 to pass through.

[0035] Specifically, a slot 40 is provided on the rear side of the clamp 63. The first solenoid valve 10 and the second solenoid valve 11 can be inserted into the slot 40 of the clamp 63 and drive the clamp 63 and the thin plate 7 to move along the X-axis. A second baffle 41 is installed at the bottom of the support frame 9. A second photoelectric switch 42 that works with the second baffle 41 is installed on the base 1. When the second photoelectric switch 42 can detect the second baffle 41, the first solenoid valve 10 near the thin plate storage mechanism 3 corresponds to the slot 40 of the lowest clamp 63.

[0036] Example 3: like Figure 10 As shown, the present invention discloses another technical solution, a fully automatic intelligent thin-layer chromatography analyzer, which differs from Embodiment 1 in that a third baffle 43 is installed at the bottom of the disk 15 near its center, and a third photoelectric switch 44 that works in conjunction with the third baffle 43 is installed on the side wall of the first Z-axis moving part 14.

[0037] Specifically, a first micro switch 45 is installed on the base 1 directly below the injector 23, and a first notch 46 is provided on the support guide rod 12 for the installation of the first micro switch 45.

[0038] Example 4: like Figure 11 , 12As shown, the present invention discloses another technical solution, a fully automatic intelligent thin-layer chromatography analyzer, which differs from Embodiment 1 in that a first servo motor 47 is fixedly installed on the front side of the developing cylinder 25, and a cylinder cover 48 is fastened to the top of the developing cylinder 25. The output end of the first servo motor 47 is connected to the front side of the cylinder cover 48, and the first servo motor 47 can drive the cylinder cover 48 to rotate. A third micro switch 49 is provided on the base 1 on the front side of the Y-axis moving part 26.

[0039] Specifically, a second servo motor 50 is fixedly installed on one side of the deployment cylinder 25 on the base 1. The output end of the second servo motor 50 is connected to the filling module 30 and can drive the filling module 30 to rotate. A vibration motor 51 is installed on the filling module 30. A first heating fan 52 facing the Luer connector 31 is also installed on the base 1. A second heating fan 53 and a first camera 54 are installed on the base 1 above the deployment cylinder 25.

[0040] Specifically, a micro pump 55 is installed on the lower surface of the base 1. The inlet of the micro pump 55 is connected to the bottom of the expansion cylinder 25 through a pipe. A waste expansion agent collection box 56 is inserted on the front side of the base 1 at a position corresponding to the expansion cylinder 25. An outlet 57 is also provided on the front side of the expansion cylinder 25.

[0041] Example 5: like Figure 13 , 14 As shown, the present invention discloses another technical solution, a fully automatic intelligent thin-layer chromatography analyzer, which differs from Embodiment 1 in that the dark box 32 is provided with an outlet and an inlet on the left and right sides respectively, and the front end of the support platform 33 is fixedly installed with a fourth servo motor 58 and a third servo motor 59 by a bracket. The output end of the fourth servo motor 58 is connected to an outlet door 60 that can block the outlet, and the output end of the third servo motor 59 is connected to an inlet door 61 that can block the inlet. Furthermore, a notch 62 for supporting the clamp 63 is opened on the rear side of the upper surface of the support platform 33.

[0042] The working process of this embodiment is as follows: 1. The thin-layer plates 7 inserted in the fixture 63 are sequentially placed into the plate compartment 13. The Y-axis motor 87 rotates, driving the development cylinder 25 to move until the third micro switch 49 detects a signal and stops. The first servo motor 47 rotates, driving the cylinder cover 48 to rotate until the cylinder cover 48 is perpendicular to the cylinder opening of the development cylinder 25 and stops. The second servo motor 50 rotates, driving the filling module 30 to move to directly above the development cylinder 25 and stops. According to the experimental requirements, the system selects a suitable developing agent and injects it into the development cylinder 25. After the developing agent filling is completed, the channel vibration motor 51 for the developing agent filling starts high-frequency operation. Vibration causes the Luer connector 31 of the spreading agent channel to shake off most of the residual adsorbed spreading agent. The second servo motor 50 rotates, driving the spreading module 30 to move directly above the first heating fan 52 and stop. The first heating fan 52 starts, drying the residual spreading liquid remaining on the outer wall of the Luer connector 31 of the spreading solution channel. The Y-axis motor 87 rotates, driving the spreading cylinder 25 to move 10mm in the direction of the Y-axis motor 87 and stop. The first servo motor 47 rotates, driving the cylinder cover 48 to rotate until the cylinder cover 48 completely covers and is tightly attached to the cylinder opening of the spreading cylinder 25. At the same time, the X-axis motor 67 rotates, driving the first solenoid valve 10 to move behind the thin plate 7 inserted in the clamp 63. The first solenoid valve 10, through its electromagnetic shaft, inserts into the clamp 63, driving the thin plate 7 to move above the support surface of the first micro switch 45.

[0043] 2. Begin sample spotting: The first Z-axis motor 72 rotates, driving the disc 15 to rotate, rotating the cotton ball 19 to a position directly below the injector 23 and stopping. The second Z-axis motor 76 drives the third Z-axis moving component 22 downwards until the needle of the injector 23 inserts into the cotton ball 19. Subsequently, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downwards, emptying the residual solution inside the sampling needle. After the residual solution is emptied, the second Z-axis motor 76 rotates, driving the third Z-axis moving component 22 upwards to the origin of the second Z-axis moving component (the first baffle 79 moves to the position where the first photoelectric switch 78 detects the signal). The first Z-axis motor 72 rotates, driving the disc 15 to rotate, rotating the first vial 16 to a position directly below the injector 23 and stopping. The second Z-axis motor 76 rotates, driving the third Z-axis moving component 22 downwards until the needle of the injector 23 inserts into the cotton ball 19. The third Z-axis moving part 22 moves downward until the needle of the injector 23 is inserted below the liquid level inside the first vial 16. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 upward, drawing 8 μL of washing solution. The second Z-axis motor 76 drives the third Z-axis moving part 22 upward to its origin. The first Z-axis motor 72 rotates, driving the disc 15 to rotate, rotating the second vial 17 to a stop directly below the injector 23. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downward, emptying the washing solution inside the sampling needle. After the washing solution is empty, the second Z-axis motor 76 drives the third Z-axis moving part upward to its origin. The first Z-axis motor 76 then rotates again. 2. Rotation drives the disc 15 to rotate, rotating the first vial 16 to a position directly below the injector 23 and stopping. The second Z-axis motor 76 drives the third Z-axis moving component downwards until the injector 23 needle is inserted below the liquid level inside the first vial 16. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 upwards, drawing 8 μL of washing solution. The second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disc 15 to rotate, rotating the second vial 17 to a position directly below the injector 23 and stopping. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downwards, emptying the washing solution inside the sampling needle. The system empties out the fluid. The second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, causing the disk 15 to rotate, rotating the cotton ball 19 until it stops directly below the injector 23. The second Z-axis motor 76 drives the third Z-axis moving component downwards until the injector 23 needle inserts into the cotton ball 19. Then, the second Z-axis motor 76 rotates, driving the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, causing the disk 15 to rotate, rotating the third vial 18 until it stops directly below the injector 23. The second Z-axis motor 76 rotates, driving the third Z-axis moving component downwards until the injector 23 needle inserts below the liquid level inside the third vial 18. Finally, the third Z-axis motor 83 rotates.The pressure block 85 pushes the sampling needle pusher 24 upwards, drawing up 8 μL of the test solution. The second Z-axis motor 76 drives the third Z-axis moving component upwards to the origin of the second Z-axis moving component. The first Z-axis motor 72 rotates, driving the disk 15 to rotate, rotating the second vial 17 to stop directly below the injector 23. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle pusher 24 downwards, emptying the test solution inside the sampling needle. After the test solution is emptied, the second Z-axis motor 76 drives the third Z-axis moving component upwards to the origin of the second Z-axis moving component. The first Z-axis motor 72 rotates, driving the disk 15 to rotate, rotating the third vial 18 to stop directly below the injector 23. The second Z-axis motor 76 drives the third Z-axis moving component... The syringe 23 needle is moved downwards until it is below the liquid level inside the third vial 18. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle pusher 24 upwards, drawing up 8 μL of the test solution. The second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disc 15 to rotate, rotating the second vial 17 until it stops directly below the syringe 23. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle pusher 24 downwards, emptying the test solution inside the sampling needle. After the test solution is emptied, the second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disc 15 to rotate. The first Z-axis motor 72 rotates, causing the disc 15 to rotate, and the second Z-axis motor 76 drives the third Z-axis moving component downwards until the needle of the injector 23 is inserted into the cotton ball 19. Then, the second Z-axis motor 76 rotates, causing the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, causing the disc 15 to rotate, and the third vial 18 rotates to its origin. The second Z-axis motor 76 drives the third Z-axis moving component downwards until the needle of the injector 23 is inserted below the liquid level inside the third vial 18. Then, the third Z-axis motor 83 rotates, causing the pressure block 85 to push the sampling needle push rod 24 upwards, drawing up 8 μL of the test solution. The second Z-axis motor 76 then drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disk 15 to rotate, rotating the second vial 17 to a position directly below the injector 23 and stopping. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downwards, discharging 2μL of the test solution from inside the sampling needle. The second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disk 15 to rotate, rotating the cotton ball 19 to a position directly below the injector 23 and stopping. The second Z-axis motor 76 drives the third Z-axis moving component downwards until the needle of the injector 23 inserts into the cotton ball 19. Then, the second Z-axis motor 76 rotates, driving the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates...The rotating disc 15 rotates until the fan-shaped through-hole 20 on the disc 15 is directly below the injector 23 and stops. The second Z-axis motor 76 drives the third Z-axis moving component downward until the injector 23 needle presses against the thin-layer plate 7 and the first microswitch 45 detects the signal and stops. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downward, discharging 2μL of the test solution inside the sampling needle onto the thin-layer plate 7. Then, the second Z-axis motor 76 drives the third Z-axis moving component upward 20mm. The X-axis motor 67 rotates, driving the first solenoid valve 10, thereby moving the thin-layer plate 7 13mm in the direction of the X-axis motor. The second Z-axis motor 76 drives the third Z-axis moving component downward until the injector 23 needle presses against the thin-layer plate 7 and the first microswitch 45 detects the signal and stops. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sampling needle push rod 24 downward, discharging 2μL of the test solution. The solution to be tested inside the sample needle is transferred onto the thin-layer plate 7. Then, the second Z-axis motor rotates, driving the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disk 15 to rotate, rotating the second vial 17 to a position directly below the injector 23 and stopping. Then, the third Z-axis motor 83 rotates, driving the pressure block 85 to push the sample needle pusher 24 downwards, emptying the solution to be tested inside the sample needle. After the solution is emptied, the second Z-axis motor 76 drives the third Z-axis moving component upwards to its origin. The first Z-axis motor 72 rotates, driving the disk 15 to its zero position and stopping (the third baffle 43 rotates until the third photoelectric switch 44 detects a signal). The third Z-axis motor 83 rotates, driving the pressure block 85 to push the sample needle pusher 24 upwards until the second microswitch 86 detects a signal and stops. The first Z-axis motor 72 rotates again, stopping only after the third photoelectric switch 44 detects a signal.

[0044] 3. The first servo motor 47 rotates, causing the cylinder head 48 to rotate until the cylinder head 48 is perpendicular to the cylinder port of the unfolded cylinder 25 and stops.

[0045] 4. Begin displaying the exhibition boards: The X-axis motor 67 rotates, driving the first solenoid valve 10, which in turn moves the thin-layer plate 7 towards the X-axis motor. The thin-layer plate 7 stops when it reaches the center of the unfolding cylinder 25. The first solenoid valve 10 deactivates and the solenoid shaft retracts. The Y-axis motor 87 rotates, driving the unfolding cylinder 25 to move 53mm towards the Y-axis motor 87 and then stops. The first servo motor 47 rotates, driving the cylinder cover 48 to rotate until it completely covers and is tightly attached to the opening of the unfolding cylinder 25. After 150 seconds, the first servo motor 47 rotates, driving the cylinder cover 48 to rotate until it is perpendicular to the opening of the unfolding cylinder 25 and then stops. The Y-axis motor rotates, driving the unfolding cylinder 25 to move 53mm towards the third micro switch 49 and then stops. The first camera 54 takes a picture to record the front edge of the unfolding reagent. The second heating fan 53 starts, heating and drying the unfolding reagent on the surface of the thin-layer plate 7. When the developing solvent needs to be replaced, the micro pump 55 starts working, emptying the developing solvent in the developing cylinder 25 into the waste developing solvent collection box 56. The second heating fan 53 starts to dry the remaining developing solvent in the developing cylinder 25. The Y-axis motor 87 rotates, driving the developing cylinder 25 to move until the third micro switch 49 detects a signal and stops. The first servo motor 47 rotates, driving the cylinder cover 48 to rotate until the cylinder cover 48 is perpendicular to the cylinder opening of the developing cylinder 25 and stops. The second servo motor 50 rotates, driving the filling module 30 to move to directly above the developing cylinder 25 and stops. According to the experimental requirements, the system selects the appropriate developing solvent to inject into the developing cylinder 25 (developing solvent 1 is added by the peristaltic pump 1 through pipeline line 1, and so on for developing solvent 2, developing solvent 3, developing solvent 4, and developing solvent 5). The initial solution of the newly added developing agent is discharged from the developing cylinder 25 to the waste developing agent collection box 56 by the micro pump 55. After the developing agent is added, the vibration motor 51 of the developing agent channel starts to vibrate at high frequency, causing the Luer connector 31 of the developing agent channel to shake off most of the residual adsorbed developing agent. The second servo motor 50 rotates and drives the developing module 30 to move to the position directly above the first heating fan 52 and stops. The first heating fan 52 starts and dries the residual developing liquid on the outer wall of the Luer connector 31 of the developing solution channel. The Y-axis motor rotates and drives the developing cylinder 25 to move 10mm in the direction of the Y-axis motor and stops. The first servo motor 47 rotates and drives the cylinder cover 48 to rotate until the cylinder cover 48 completely covers and is tightly attached to the cylinder opening of the developing cylinder 25.

[0046] 5. Start data collection: The third servo motor 59 rotates, causing the entrance door 61 of the dark box 32 to open. The 254nm ultraviolet lamp 35 is powered on, and the X-axis motor 67 rotates, causing the second solenoid valve 11 to move to the rear of the thin-layer plate 7 clamp 63. The second solenoid valve 11 extends its solenoid shaft and inserts it into the center of the rear of the thin-layer plate 7 clamp 63, causing the thin-layer plate 7 to move towards the X-axis motor 67. The thin-layer plate 7 moves to the center of the internal support platform 33 of the dark box 32. The second solenoid valve 11 then retracts its solenoid shaft, and the X-axis motor rotates, causing the second solenoid valve 11 to move in the opposite direction to the X-axis motor 67. The third servo motor 59 rotates, causing the dark box 32 to open. The entrance door 61 closes, the second camera 34 takes a picture of the thin plate 7 under the illumination of the 254nm ultraviolet lamp 35, and the picture is taken. The fourth servo motor 58 rotates to open the exit door 60 of the dark box 32. The X-axis motor 67 rotates to move the second solenoid valve 11 in the direction of the X-axis motor. At the same time, the second solenoid valve 11 extends through the electromagnetic shaft, pushing the clamp 63 to move the thin plate 7 in the direction of the X-axis motor 67 until the clamp 63 and the thin plate 7 slide off the exit of the dark box 32. The fourth servo motor 58 rotates to close the exit door 62 of the dark box 32. The X-axis motor 67 rotates until it stops at the zero point of the X-axis moving part (the second baffle 41 moves to the second photoelectric switch 42 to detect the signal).

[0047] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A fully automated intelligent thin-layer chromatography analyzer, comprising a base (1), characterized in that: The base (1) is provided with a thin-layer plate moving mechanism (2), a thin-layer plate storage mechanism (3), a sample dispensing mechanism (4), a display plate mechanism (5) and an image acquisition mechanism (6). The thin-layer plate moving mechanism (2) can drive the thin-layer plate (7) from the thin-layer plate storage mechanism (3) through the sample dispensing mechanism (4), the display plate mechanism (5) and the image acquisition mechanism (6) in sequence. The thin-layer plate moving mechanism (2) includes an X-axis moving component (8), a support frame (9), a first solenoid valve (10), a second solenoid valve (11), and a support guide rod (12). The support frame (9) is connected to the X-axis moving component (8), and the X-axis moving component (8) can drive the support frame (9) to move along the X-axis direction. The first solenoid valve (10) and the second solenoid valve (11) are installed on the top of the support frame (9) along the X-axis direction, and both the first solenoid valve (10) and the second solenoid valve (11) can drive the thin-layer plate (7) to move along the support guide rod (12). The support guide rod (12) is set along the X-axis direction. The thin-layer plate storage mechanism (3) includes a plate compartment (13), which is installed above one side of the X-axis moving part (8). The rear end of the thin-layer plate (7) is inserted into the clamp (63), and the plate compartment (13) is provided with a vertical channel for placing the thin-layer plate (7) and the clamp (63). The lowest thin-layer plate (7) and the clamp (63) are supported by a support guide rod (12). The sampling mechanism (4) includes a liquid storage component and a sampling component; the liquid storage component includes a first Z-axis moving part (14), a disk (15), a first vial (16) for storing washing solution, a second vial (17) for storing waste liquid, a third vial (18) for storing the liquid to be tested, and a cotton ball (19). The first Z-axis moving part (14) can drive the disk (15) to rotate. The first vial (16), the second vial (17), the third vial (18), and the cotton ball (19) are all equipped with At the edge of the upper surface of the disk (15), and evenly distributed circumferentially along the axis of the disk (15), a fan-shaped through hole (20) is provided on the disk (15); the sample dispensing assembly includes a second Z-axis moving part (21), a third Z-axis moving part (22) and a sample injector (23). The second Z-axis moving part (21) can drive the third Z-axis moving part (22) to move up and down along the Z-axis direction. The third Z-axis moving part (22) can drive the sample dispensing needle push rod (24) of the sample injector (23) to move up and down along the Z-axis direction. The display panel mechanism (5) includes a display panel assembly and a liquid injection assembly. The display panel assembly includes a display cylinder (25) and a Y-axis moving part (26). The Y-axis moving part (26) can drive the display cylinder (25) to move back and forth along the Y-axis direction. The liquid injection assembly includes a magnetic stirrer (27), a developing agent bottle (28), a peristaltic pump (29), a filling module (30), and a Luer connector (31). The Luer connector (31) is installed on the filling module (30). The peristaltic pump (29) can inject the developing agent in the developing agent bottle (28) into the display cylinder (25) through the Luer connector (31). The image acquisition mechanism (6) includes a dark box (32), a support platform (33), a second camera (34) and an ultraviolet lamp (35). The dark box (32) is covered on the outside of the support platform (33), and the second camera (34) and the ultraviolet lamp (35) are both installed on the top of the dark box (32).

2. The fully automated intelligent thin-layer chromatography analyzer according to claim 1, characterized in that: The clamp (63) has an integrally provided limiting slider (37) on its side wall, and the inner wall of the plate compartment (13) has a vertically provided limiting groove (36) that works with the limiting slider (37), and the bottom of the clamp (63) has a slot (38) along the X-axis.

3. The fully automated intelligent thin-layer chromatography analyzer according to claim 2, characterized in that: Two support guide rods (12) are provided at the front and back. One end of each support guide rod (12) is connected to the bottom of the plate hopper (13). The other end of the support guide rod (12) is installed on the side wall of the dark box (32). The rear support guide rod (12) is located in the slot (38) at the bottom of the clamp (63). The clamp (63) can move along the rear support guide rod (12) through the slot (38). The bottom of the thin plate (7) is placed on the front support guide rod (12). The front support guide rod (12) has a second notch (39) corresponding to the position of the unfolding cylinder (25). The bottom of the plate hopper (13) facing the sampling mechanism (4) has an opening for the bottom thin plate (7) and the clamp (63) to pass through.

4. The fully automated intelligent thin-layer chromatography analyzer according to claim 3, characterized in that: The clamp (63) has a slot (40) on its rear side. The first solenoid valve (10) and the second solenoid valve (11) can be inserted into the slot (40) of the clamp (63) and drive the clamp (63) and the thin plate (7) to move along the X-axis. The bottom of the support frame (9) is equipped with a second baffle (41). The base (1) is equipped with a second photoelectric switch (42) that works with the second baffle (41). When the second photoelectric switch (42) can detect the second baffle (41), the first solenoid valve (10) near the thin plate storage mechanism (3) corresponds to the slot (40) of the lowest clamp (63).

5. The fully automated intelligent thin-layer chromatography analyzer according to claim 1, characterized in that: A third baffle (43) is installed at the bottom of the disk (15) near its center, and a third photoelectric switch (44) that works in conjunction with the third baffle (43) is installed on the side wall of the first Z-axis moving part (14).

6. The fully automated intelligent thin-layer chromatography analyzer according to claim 5, characterized in that: A first micro switch (45) is installed on the base (1) directly below the injector (23), and a first notch (46) for installing the first micro switch (45) is provided on the support guide rod (12).

7. The fully automated intelligent thin-layer chromatography analyzer according to claim 1, characterized in that: The first servo motor (47) is installed on the front side of the deployment cylinder (25), and the cylinder cover (48) is fastened to the top of the deployment cylinder (25). The output end of the first servo motor (47) is connected to the front side of the cylinder cover (48), and the first servo motor (47) can drive the cylinder cover (48) to rotate. A third micro switch (49) is provided on the base (1) on the front side of the Y-axis moving part (26).

8. The fully automated intelligent thin-layer chromatography analyzer according to claim 7, characterized in that: A second servo motor (50) is provided on one side of the deployment cylinder (25) on the base (1). The output end of the second servo motor (50) is connected to the filling module (30) and can drive the filling module (30) to rotate. A vibration motor (51) is installed on the filling module (30). A first heating fan (52) facing the Luer joint (31) is also installed on the base (1). A second heating fan (53) and a first camera (54) are installed on the base (1) above the deployment cylinder (25).

9. The fully automated intelligent thin-layer chromatography analyzer according to claim 8, characterized in that: A micro pump (55) is installed on the lower surface of the base (1). The inlet of the micro pump (55) is connected to the bottom of the expansion cylinder (25) through a pipe. A waste expansion agent collection box (56) is inserted on the front side of the base (1) at the position corresponding to the expansion cylinder (25). An outlet (57) is also provided on the front side of the expansion cylinder (25).

10. The fully automated intelligent thin-layer chromatography analyzer according to claim 1, characterized in that: The dark box (32) has an outlet and an inlet on its left and right sides, respectively. The front end of the support platform (33) is equipped with a fourth servo motor (58) and a third servo motor (59) via a bracket. The output end of the fourth servo motor (58) is connected to an outlet door (60) that can block the outlet. The output end of the third servo motor (59) is connected to an inlet door (61) that can block the inlet. A notch (62) for supporting the clamp (63) is opened on the rear side of the upper surface of the support platform (33).

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