Chemiluminescence immunoassay analyzer

Abstract

The application discloses a chemiluminescence immunoassay analyzer, which at least comprises a material preparation module for placing one or more suction head supports and reagent strips; a film pressing module for sample pretreatment, which comprises a film pressing plate and a film piercing needle arranged on the film pressing plate; and a pipetting module for liquid suction / dispensing operation, which comprises a row pipetting pump and a suction head withdrawal guide sleeve; the row pipetting pump is controlled to drive the suction head withdrawal guide sleeve to take out a filter membrane pressing rod from the suction head support and place the filter membrane pressing rod in a filter rod cabin, or to take out a suction head and perform liquid suction / dispensing operation; the film pressing plate is controlled to press the filter membrane pressing rod against the filter rod cabin, or to open a film for each cabin in the reagent strip through the film piercing needle. The application can perform zero-pollution operation on multiple groups of reagent strips, and the modular layered structure design can simultaneously complete multiple index analysis in single detection, and the equipment is small in size, simple in operation and low in cost.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of medical detection equipment, and particularly relates to a small full-automatic chemiluminescence immunoassay analyzer suitable for combined detection of invasive fungi and inflammation indexes. BACKGROUND

[0002] Chemiluminescence immunoassay (CLIA) is a highly sensitive analysis technique that combines immunological specificity and the ultra-high sensitivity of chemiluminescence. This technique has been widely used in clinical diagnosis, especially in the detection of tumor markers, hormones, infectious diseases, etc. The full-automatic chemiluminescence immunoassay analyzer automates this technique, realizing the full-process automation from sample loading, reagent addition, reaction incubation, detection to result reporting. This greatly improves the detection efficiency and accuracy, and reduces human operation errors. The technology can realize the detection of tumor markers, hormones, infectious diseases and invasive fungal infections, among which invasive fungal infection is a serious threat to the life of patients with low immune function. Traditional fungal detection methods, such as culture method, are time-consuming and have low sensitivity, which cannot meet the clinical demand for rapid and accurate diagnosis. The full-automatic chemiluminescence immunoassay technology provides a new powerful tool for the diagnosis of invasive fungal infection with its high sensitivity, high specificity, fast result, etc.

[0003] Invasive fungal infection (IFI) and inflammation often coexist, and their clinical manifestations are highly overlapping, making it very difficult to diagnose IFI in the early stage. Therefore, combining invasive fungal detection with inflammation index detection can more comprehensively evaluate the patient's condition and improve the accuracy of diagnosis. However, the detection environment for invasive fungal detection is very high, mainly because fungal spores exist widely in the air and are easy to contaminate, affecting the accuracy of the detection results. Therefore, it is crucial to establish a sterile, clean and bio-safety standard-compliant detection environment. Therefore, it has certain clinical application significance to carry out invasive fungal infection (IFI) and inflammation combined detection through the full-automatic chemiluminescence immunoassay analyzer.

[0004] However, the existing full-automatic chemiluminescence immunoassay analyzer still has significant defects:

[0005] (1) The traditional instrument adopts a steel needle sampling system, which can reduce contamination through cleaning, but cannot completely eliminate it, resulting in sample residues on the steel needle surface and affecting the subsequent detection results of other samples. Although there are instruments that use a disposable suction head system, which can avoid sample contamination, the subsequent experimental process still needs to share the liquid path for magnetic separation and cleaning steps, which still has the risk of cross-contamination, especially for high-environmental requirement projects such as invasive fungal detection.

[0006] (2) The existing instrument relies on a liquid path system for reagent dispensing and cleaning, which needs to be regularly and strictly maintained. The maintenance of the liquid path system is complex, otherwise microorganisms may breed inside the pipeline or the pipeline may be blocked, which not only increases the operation and maintenance cost, but also may lead to out-of-control detection results, and the technical requirements for the operator are high.

[0007] (3) The traditional instrument is bulky and costly, which is suitable for large sample volume in large hospitals, but for secondary hospitals or community hospitals, the large-scale equipment does not match the basic needs, the procurement and use cost is too high, and the detection throughput far exceeds the actual demand, resulting in waste of resources.

[0008] (4) IFI and inflammatory response have high overlap in clinical manifestations, and single index detection is easy to misdiagnose, and the combined detection demand is not met. The existing equipment is difficult to realize the synchronous combined detection of fungi and inflammatory indicators, resulting in low diagnosis and treatment efficiency.

[0009] Therefore, a small, liquid-free, highly reliable fully automatic chemiluminescence immunoassay analyzer is needed to solve the problems of cross contamination, complex maintenance, equipment applicability and combined detection demand, and to meet the actual needs of basic medical institutions. SUMMARY

[0010] The present application provides a chemiluminescence immunoassay analyzer, which solves the technical problems of cross contamination, complex maintenance, weak equipment applicability and poor combined detection performance of the existing fully automatic chemiluminescence immunoassay analyzer.

[0011] To solve at least one of the above technical problems, the technical solution adopted by the present application is:

[0012] A chemiluminescence immunoassay analyzer, at least comprising:

[0013] A material preparation module for placing one or more suction head supports and reagent strips;

[0014] A film pressing module for sample pretreatment, including a film pressing plate and a film piercing needle arranged on the film pressing plate;

[0015] A pipetting module for liquid suction / dispensing operation, including a row pipetting pump and a suction head withdrawal guide sleeve;

[0016] Controlling the row pipetting pump to drive the suction head withdrawal guide sleeve to take out the filter membrane pressing rod from the suction head support and place it in the filter rod cabin, or take out the suction head and perform liquid suction / dispensing operation;

[0017] Controlling the film pressing plate to press the filter membrane pressing rod into the filter rod cabin, or opening the film in each cabin of the reagent strip through the film piercing needle.

[0018] Further, the material preparation module further comprises a bracket carrying the suction head support and the reagent strip, a gantry bracket fixing the bracket, a lead screw motor I driving the gantry bracket to slide relative to the linear guide rail, and the lead screw motor I drives the bracket to move along the length direction of the bracket through a lead screw block I.

[0019] Opposite guide wheel grooves are arranged on the back side edges of the bracket, and at least two groups of guide wheel supports cooperate with the guide wheel grooves to provide fixed support when the bracket reciprocally slides.

[0020] Further, the film pressing module and the pipetting module are arranged on the upper frame, and the film pressing module is close to one side of the suction head support; the film pressing module and the pipetting module are arranged across the width direction of the upper frame; wherein,

[0021] The film pressing module further comprises a fixed side plate for fixing the film pressing plate and a film pressing motor for driving the film pressing plate, and a film pressing guide rail is arranged on the fixed side plate and cooperates with a film pressing block on the film pressing plate; the film pressing motor drives the film pressing plate to vertically move up and down along the film pressing guide rail through a film pressing lead screw.

[0022] Further, the film pressing plate is arranged directly above the filter rod cabin in the suction head support; at least one film piercing needle is arranged on the side of the film pressing plate away from the fixed side plate, and the film piercing needle is arranged along the length direction of the film pressing plate, and the position of the film piercing needle is matched with the arrangement of the reagent strip.

[0023] Further, the pipetting module further comprises a pipetting frame for fixing the row pipetting pump, a lifting motor for driving the row pipetting pump to move up and down, and a suction and discharge motor for controlling the suction and discharge of the row pipetting pump, wherein,

[0024] The row pipetting pump is fixed on the outer side wall of the pipetting frame;

[0025] A pipetting vertical plate and a connecting plate are arranged in a cross manner above the row pipetting pump, and the pipetting vertical plate is connected to the vertical sliding rail on the pipetting frame through a sliding block;

[0026] The lifting motor drives the lifting lead screw through a belt transmission to drive the lead screw block on the pipetting vertical plate to drive the pipetting vertical plate and the row pipetting pump to move up and down synchronously;

[0027] The suction and discharge motor is fixed on the pipetting vertical plate, and drives the lead screw block arranged on the connecting plate through a suction and discharge lead screw to work, drives the connecting plate to move away from / push the piston on the row pipetting pump to rise / down, so that the suction head sucks / pumps liquid.

[0028] Further, the two ends of the pipette stand in the width direction are provided with outwardly arranged U-shaped grooves, a screw block two is installed in the U-shaped groove, a linear slide block two matched with the linear guide rail two is arranged on the lower bottom surface of the U-shaped groove, and the screw motor two drives the pipette screw to rotate to drive the screw block two to move along the linear guide rail two together with the pipette stand and the row pipette pump one.

[0029] Further, the material preparation module is arranged on the lower frame, and a heating module for high-temperature heating of samples in a reagent strip, a magnetic separation module for separating reagents, and an optical acquisition module for optical acquisition of information are further arranged on the lower frame, wherein the heating module is arranged close to one side of the material preparation module, and the magnetic separation module is located between the heating module and the optical acquisition module.

[0030] Further, the heating module comprises a base plate, a heating motor, a heating unit, and a support plate for fixing the heating unit; a vertical guide rail is arranged on the base plate and is in sliding connection with the heating unit; the heating motor drives the support plate to lift and lower through a cam disc, so as to drive the heating unit to move vertically up and down.

[0031] Further, the optical acquisition module comprises an acquisition stepping motor, a linear guide rail three, a detection slide table, and a photon counter; the linear guide rail three is fixed horizontally on the lower frame; the acquisition stepping motor is fixed on the linear guide rail three and drives the detection slide table to slide along the linear guide rail three through a belt transmission.

[0032] Further, a shell is further arranged, a hatch corresponding to the material preparation module is arranged on the head of the shell, an air outlet is arranged on the back of the shell, an air inlet is arranged on the bottom of the shell, and an external interface is arranged on the single side wall surface of the shell; a power supply module and a main control module are further arranged on the frame in the shell.

[0033] The chemical luminescence immunoassay analyzer designed in the application adopts a liquid-free design of independent row pipette pump and disposable suction head, which fundamentally eliminates the risk of sample pollution, significantly improves the detection accuracy, and realizes miniaturization and desktop deployment through modular integrated design; meanwhile, the analyzer supports multi-index joint detection, greatly improves the detection efficiency, and has high reliability and cost performance, which is suitable for the needs of precision, simplicity and economical detection of primary medical institutions. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 is a perspective view of the analyzer in the application;

[0035] Figure 2 is a perspective view of the analyzer in the application from another angle;

[0036] Figure 3 is a perspective view of the lower frame in the present application;

[0037] Figure 4 is a side view of the lower frame in the present application;

[0038] Figure 5 is a top view of the lower frame in the present application;

[0039] Figure 6 is a perspective view of the suction head support in the present application;

[0040] Figure 7 is a top view of the suction head support in the present application;

[0041] Figure 8 is a front view of the suction head support in the present application;

[0042] Figure 9 is a perspective view of Figure 8 is an enlarged view of part A in

[0043] Figure 10 is a perspective view of the spring and the positioning bump in the suction head support;

[0044] Figure 11 is a perspective view of the suction head support cooperating with the cradle;

[0045] Figure 12 is a perspective view of the reagent strip in the present application;

[0046] Figure 13 is a perspective view of the upper frame in the present application;

[0047] Figure 14 is a perspective view of the upper frame in the present application from another angle;

[0048] Figure 15 is a perspective view of the film pressing module in the present application in a state on the lower support;

[0049] Figure 16 is a perspective view of the film pressing module in the present application;

[0050] Figure 17 is a rear perspective view of the film pressing module in the present application;

[0051] Figure 18 is a side view of the film pressing module in the present application;

[0052] Figure 19 is a perspective view of the pipetting module in the present application in an initial state;

[0053] Figure 20 is a perspective view of the pipetting module in the present application in a lowered state;

[0054] Figure 21 is a perspective view of the desorption head guide sleeve and the pipetting pump head in the present application;

[0055] Figure 22 is a side view of the pipetting module in the present application;

[0056] Figure 23 is a perspective view of the heating module in the present application;

[0057] Figure 24 is a schematic diagram of the driving structure of the heating module in the present application;

[0058] Figure 25 is a state diagram when the heating module in the present application is heating;

[0059] Figure 26 is a perspective view of the optical acquisition module in the initial state in the present application;

[0060] Figure 27 is a perspective view of the back of the optical acquisition module in the present application;

[0061] Figure 28 is a perspective view of the optical acquisition module in the detection state in the present application;

[0062] Figure 29 is a perspective view of the housing in the present application;

[0063] Figure 30 is a side view of the housing in the present application;

[0064] Figure 31 is a bottom view of the housing in the present application;

[0065] Figure 32 is a rear view of the housing in the present application.

[0066] In the drawings:

[0067] 10, housing; 11, upper frame; 12, lower frame; 13, hatch; 14, air outlet; 15, air inlet; 16, external interface; 20, material preparation module; 21, suction head support; 211, support; 212, heating cabin; 213, filter rod cabin; 214, suction head cabin; 215, suction head; 216, filter membrane pressing rod; 217, positioning lock catch; 218, elastic lock catch; 2181, elastic sheet; 2182, positioning convex point; 2183, anti-skid point; 2184, through hole; 22, reagent strip; 221, strip body; 222, sample cabin; 223, component cabin; 224, reaction cup; 225, handle; 23, bracket; 231, clamping table; 24, gantry support; 25, linear guide rail one; 26, screw block one; 27, screw motor one; 28, guide wheel support; 29, guide wheel groove; 30, membrane pressing module; 31, membrane pressing motor; 32, membrane pressing plate; 33, fixed side plate; 34, membrane pressing screw; 35, membrane pressing block; 36, membrane pressing bearing seat; 37, membrane piercing needle; 38, membrane pressing coupling; 40, pipetting module; 41, pipetting support; 42, row pipetting pump; 43, suction and discharge motor; 44, lifting motor; 45, linear guide rail two; 46, screw block two; 47, screw motor two; 48, suction head return guide sleeve; 49, pipetting vertical plate; 410, vertical guide rail one; 411, pipetting pump head; 412, linear block two; 413, connecting plate; 50, heating module; 51, base plate; 52, heating motor; 53, support plate; 54, cam disc; 55, vertical guide rail two; 56, heating unit; 60, magnetic separation module; 70, optical acquisition module; 71, acquisition stepping motor; 72, linear guide rail three; 73, detection sliding table; 731, detection stepping motor; 732, vertical guide rail three; 733, telescopic sleeve; 734, mounting table; 735, code scanner; 736, laser detection head; 74, photon counter; 75, darkroom column; 76, circuit board; 80, power module; 90, main control module. DETAILED DESCRIPTION

[0068] The present application will be described in detail below with reference to the drawings and specific embodiments.

[0069] The present embodiment proposes a chemiluminescence immunoassay analyzer, such as Figures 1-2As shown, the device includes a housing 10, in which a material preparation module 20 for placing a tip holder 21 and a reagent strip 22, a membrane pressing module 30 for breaking the membrane of each chamber in the reagent strip 22 and filtering the liquid in the heating chamber 212 out of the supernatant through the filter membrane pressing rod 216, a pipetting module 40 for taking and placing the tip 215 and the filter membrane pressing rod 216, sucking and discharging reagents, and transferring samples, a heating module 50 for high-temperature heating of samples to achieve sample pretreatment, a magnetic separation module 60 for adsorption operation of magnetic particles in reagents, an optical acquisition module 70 for detecting and reading the experimental results, a power supply module 80 for powering the entire instrument, and a main control module 90 for overall control of the instrument are arranged. Among them, the material preparation module 20, the heating module 50, the magnetic separation module 60 and the main control module 90 are arranged on the lower frame 12 in the housing 10; the membrane pressing module 30, the pipetting module 40, the optical acquisition module 70 and the power supply module 80 are arranged on the upper frame 11.

[0070] Among them, the material preparation module 20 is located at one end of the lower frame 12, the heating module 50 is arranged close to the material preparation module 20, the magnetic separation module 60 is arranged away from the material preparation module 20, the material preparation module 20 is arranged along the length direction of the lower frame 12, and the heating module 50 and the magnetic separation module 60 are arranged along the width direction of the lower frame 12. The membrane pressing module 30 is arranged at one end of the upper frame 11 and close to the material preparation module 20, the pipetting module 40 is arranged in the middle of the upper frame 11, and the optical acquisition module 70 is arranged at the tail side of the upper frame 11, i.e. away from the membrane pressing module 30. All modules are arranged in the housing 10, and the material preparation module 20 is located at the head of the housing 10, and the optical acquisition module 70 is located at the tail of the housing 10. The overall structure is reasonable, small and compact, and is completely suitable for desktop deployment in primary hospitals.

[0071] The control of the row pipetting pump 42 drives the tip withdrawal guide sleeve 48 to take out the filter membrane pressing rod 216 from the tip holder 21 and place it in the filter rod chamber 213 on the tip holder 21, or to take out the tip 215 from the tip holder 21 and perform liquid suction / discharging operation in the reagent strip 22. The membrane pressing plate 32 in the membrane pressing module 30 can also press the filter membrane pressing rod 216 into the filter rod chamber 213, or the membrane breaking needle 37 can break the membrane of each chamber in the reagent strip 22. The analyzer proposed in the present application is based on a liquid-free and highly integrated module design, can support multi-group five-plex detection of invasive fungi and inflammation indicators, simultaneously complete multiple index analysis in a single detection, and can realize "just place and measure", significantly shortening the detection time. At the same time, it has the advantages of simple operation, high reliability and low cost, realizes the miniaturized deployment of the desktop level, and also reduces the requirement for the professional background of the operator, especially meets the detection needs of secondary hospitals and community hospitals.

[0072] like Figure 29 As shown, a display screen is provided at one end of the housing 10, and an openable door 13 corresponding to the material preparation module 20 is provided on the same side. The test strips 22 and pipette tip holders 21 are placed on the tray 23 inside the material preparation module 20 through the door 13. Samples can be separately tested via touch screen on the display screen. The display screen is controlled by the main control module 90 and is electrically connected to the material preparation module 20, the film pressing module 30, the pipetting module 40, the heating module 50, the magnetic separation module 60, the optical acquisition module 70, and the power supply module 80.

[0073] like Figure 30 As shown, several external interfaces 16 are provided on the side wall of the housing 10, corresponding to the positions of the power module 80. Meanwhile, an air inlet 15 is provided at the bottom of the housing 10, directly below the heating module 50, as shown... Figure 31 As shown, since the heating module 50 is used to denature the sample, the sample also needs to be cooled quickly after heating. Therefore, the air inlet 15 is located next to the high-temperature heating module 50 to assist in cooling.

[0074] like Figure 32 As shown, on the rear side of the housing 10 corresponding to the display screen, an air outlet 14 is provided at its upper section to exhaust hot air from inside the housing 10. Inside the housing 10, fans are installed at positions corresponding to the air outlet 14 and the air inlet 15. One fan is fixed to the lower frame 12, and the other is fixed to the upper frame 11. This ensures that the internal temperature of the instrument is the same as room temperature, preventing the internal temperature from rising and affecting reagent experiments after prolonged operation.

[0075] like Figure 2 As shown, a power supply module 80 that supplies power to the entire analyzer is located on the upper frame 11; below the power supply module 80, i.e., on the lower frame 12, a main control module 90 for controlling the step control of all modules in the entire analyzer is also configured. The power supply module 80 is located on the frame opposite the external interface 16.

[0076] like Figures 3-4As shown, the material preparation module 20 is used to place one or more suction head supports 21 and reagent strips 22, which includes a bracket 23 for placing the suction head supports 21 and reagent strips 22, a gantry support 24 for fixing the bracket 23, a linear guide rail I 25 slidingly matched with the lower end surface of the gantry support 24, and a lead screw motor I 27 for driving the gantry support 24 and moving the bracket 23 back and forth along the length direction of the lower frame 12. The bracket 23 is suspended by the gantry support 24 on both sides in the width direction, and a sliding groove slidingly matched with the linear guide rail I 25 is arranged on the lower end surface of the gantry support 24. A lead screw block I 26 is arranged on the single side outer wall surface of the gantry support 24, and the lead screw motor I 27 is arranged on the back side of the shell 10, i.e. close to the air outlet 14 side, which is threadedly matched with the lead screw block I 26 through the lead screw. Thus, the lead screw motor I 27 drives the lead screw to rotate, drives the lead screw block I 26 to move along the length direction of the bracket 23, and further drives the gantry support 24 and the bracket 23 to slide along the linear guide rail I 25.

[0077] A pair of guide wheel grooves 29 are arranged on the back side edges of the bracket 23, which are rectangular shell grooves with the lower end opening arranged directly matched with the rolling guide wheels on the guide wheel supports 28. At least two groups of symmetrically arranged guide wheel supports 28 are matched with the guide wheel grooves 29, which can provide fixed support when the bracket 23 slides back and forth. The guide wheel supports 28 are located on the inner side of the gantry support 24, and the lower end is fixed on the bottom plate of the lower frame 12, so as not to hinder the reciprocating movement of the gantry support. The two guide wheel supports 28 on the same side are arranged at intervals, one of which is arranged directly below the pressing film module 30, so as to stably support the bracket 23 upward when the pressing film plate 32 is pressed, and the other is arranged close to the heating module 50, so as to support the bracket 23 when the heating module 50 heats the sample chamber 222 in the reagent strip 22, so that the bracket 23 is stable and does not shake. Through the cooperation of the guide wheel grooves 29 and the guide wheel supports 28, the bracket 23 can be kept stable in the rapid reciprocating movement, so as to avoid the displacement of the reagent strip 22 or the suction head support 21 due to vibration, and improve the positioning accuracy and detection reliability.

[0078] As shown in FIG. 6, the bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23. The bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23. The bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23. Figure 5 As shown in FIG. 6, the bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23. The bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23. The bracket 23 is arranged on the lower layer frame 12, and the pressing film module 30 is arranged on the bracket 23.

[0079] The bracket 23 is installed on the gantry 24, which is driven by the screw motor 27 to move back and forth on the linear guide rail 25, so as to realize the reciprocating movement of the suction head bracket 21 and the reagent strip 22 between the film pressing module 30, the pipetting module 40 and the heating module 50.

[0080] As shown in Figures 6-11 The suction head bracket 21 matched with the reagent strip 22 comprises a strip-shaped bracket 211, an elastic lock catch 218 is arranged at one end of the bracket 211 for fixing and installation, and a heating cabin 212 is arranged at the other end of the bracket 211; at least one suction head cabin 214 and one filter rod cabin 213 are further arranged between the elastic lock catch 218 and the heating cabin 212. The suction head cabin 214 is arranged close to the elastic lock catch 218; the filter rod cabin 213 is arranged between the suction head cabin 214 and the heating cabin 212, and is integrally connected with the outermost suction head cabin 214 away from the elastic lock catch 218; the heating cabin 212 is arranged spaced apart from the filter rod cabin 213.

[0081] In the embodiment, the number of the suction head cabins 214 is 12, and they are arranged continuously and integrally; one suction head 215 is arranged in each suction head cabin 214, and a filter membrane pressing rod 216 is arranged in the filter rod cabin 213; all the suction heads 215 are embedded in the suction head cabins 214, and the filter membrane pressing rod 216 is embedded in the filter rod cabin 213.

[0082] As shown in Figures 8-9 The elastic lock catch 218 is a shell structure, the lower end face of which is open, the end side of the elastic lock catch 218 is vertically downward, the right angle edges of the two sides are integrally connected with the end side and the length edge of the bracket 211, and the bottom faces of the two side edges are configured as arc curved surfaces, the outer ends of the arc curved surfaces are arranged downward, the other ends close to the spring suspension ends are inclined upward, and the lower bottom faces of the two side edges are smoothly connected with the lower bottom face of the bracket 211.

[0083] The two side edges of the elastic lock catch 218 can be used as handles, which enables the operator to easily and stably take and place the entire suction head bracket 21, and improves the convenience and humanization of operation. The integral connection of the shell structure with the length edge of the bracket 211 can further increase the overall strength and rigidity of the elastic lock catch 218 and the entire suction head bracket 21, so that they are not easily deformed or damaged in repeated use and mechanical operation, and the durability and reliability are improved. In addition, the arc curved surface design of the bottom faces of the two side edges enables the elastic lock catch 218 to more smoothly contact, slide and engage with the clamping table 231 in the bracket 23 when the suction head bracket 21 is installed on the bracket 23, plays a good guiding role, reduces the installation difficulty and resistance, and ensures the installation position to be accurate and correct.

[0084] As shown in Figure 9As shown, the cross section of the clamping platform 231 matches the matching structure of the elastic lock 218, and the arc-shaped curved surface structure is conducive to forming a closer and more stable match with the clamping platform 231. When pressed by external force, this structure can better transmit force and elastically deform, so that the positioning protrusion 2182 on the elastic sheet 2181 can be more reliably buckled with the top of the clamping platform 231, thereby firmly locking the suction head support on the cradle 23, preventing it from loosening or shifting during instrument operation, such as tray movement or pipetting operation, and ensuring the stability and accuracy of the experimental process.

[0085] Specifically, the elastic sheet 2181 is arranged inside the elastic lock 218, and the fixed end of the elastic sheet 2181 is fixed to the root of the body of the elastic lock 218, i.e. on the inner bottom surface of the end of the support 211. The positioning protrusion 2182 is arranged on the lower bottom surface of the movable end of the elastic sheet 2181 and is integrally arranged with the elastic sheet 2181, and the cross section of the positioning protrusion 2182 is configured as an inverted tapered curved surface structure. The outer side arc surface of the positioning protrusion 2182 can be pushed up by the clamping platform 231 when the suction head support 21 is installed and is pushed by external force, and then the inner side arc surface thereof is in close contact with the clamping platform 231, so as to lock the elastic lock 218 on the cradle 23.

[0086] The outer side arc surface of the positioning protrusion 2182 is designed to form a slope, and when the operator presses the suction head support 21 into the cradle 23, the clamping platform 231 will first contact this arc surface, which can effectively convert the downward pressure into a component force that causes the elastic sheet 2181 to elastically deform and be lifted upward. Once the elastic sheet 2181 passes the highest point of the clamping platform 231, the inner side arc surface thereof will be in contact with the side or top of the clamping platform 231, ensuring maximum contact area and fit, and also generating strong friction and mechanical interlocking effect, so that the suction head support 21 is firmly fixed on the cradle 23. The elastic lock 218 is first lifted, then popped up, and finally locked, and a clicking sound will be produced during the entire process, so that it can be clearly perceived whether the installation is in place, and experimental failure or equipment error caused by improper installation is avoided. The structure of the elastic lock 218 realizes one-key installation and disassembly through the matching of the inverted tapered positioning protrusion 2182 and the clamping platform 231, which not only ensures the auditory and tactile feedback when installed in place, but also ensures that the locking state can still be maintained in a mechanical vibration environment, avoiding misoperation or loosening.

[0087] Preferably, the included angle θ of the positioning convex point 2182 cross section is not greater than 75° and not less than 45°, and the angle includes but is not limited to 45°, 50°, 55°, 60°, 65°, 70°, 75°. If the included angle θ is less than 45°, the side surface is too steep, the inclined surface tends to be vertical, and a great force is required to push the spring to deform during installation. Moreover, when disassembled, the inner side arc surface and the clamping table form a self-locking effect, which makes it difficult to pull out, and even the components need to be damaged to disassemble. If the included angle θ is greater than 75°, the inner side arc surface is too flat, although the installation is more labor-saving, but it will lead to insufficient locking force and friction when locking, which may not effectively resist vibration, and is prone to accidental loosening, which will weaken the resilience of the spring and reduce the reliability of the locking.

[0088] As shown in Figures 6-7 , a rectangular structure through hole 2184 is arranged on the upper end surface of the elastic lock 218, and the spring 2181 corresponds to the through hole 2184; and the width and length of the spring 2181 are smaller than the width and length of the through hole 2184. This structure not only facilitates observation of the working state of the spring 2181, but also facilitates processing, and also provides sufficient movement range for the spring 2181, so that it can be raised or retracted up and down.

[0089] At least two groups of anti-skid points 2183 are arranged on the upper end surface of the elastic lock 218, and all the anti-skid points 2183 are arranged on both sides of the through hole 2184. The anti-skid points 2183 are circular convex points, and of course they can also be designed as square or other polygonal structure convex points, as long as they can increase the friction.

[0090] As shown in Figure 9 , Figure 10 , Figure 11 , the maximum height of the elastic lock 218 is greater than the maximum height of the spring 2181, and the movable end of the spring 2181 protrudes from the position where the side edge bottom surface of the elastic lock 218 and the smooth connection section of the lower bottom surface of the support 211 are located. When the suction head support 21 is placed flat or subjected to external force in the non-installation direction, the higher elastic lock 218 will first contact the outside, thereby effectively protecting the lower spring 2181 and the precise positioning convex point 2182 thereon from accidental collision and damage, and improving the overall reliability of the suction head support. During installation, only the movable end of the spring 2181, i.e. with the positioning convex point 2182, can contact and interact with the clamping table 231 on the bracket 23; if the movable end of the spring does not protrude or is surrounded by the structure of the elastic lock 218, it cannot be engaged with the clamping table 231.

[0091] As Figure 10 shown, the positioning convex point 2182 is configured as a shell structure, that is, a hollow nest is arranged in the middle. This structure design realizes lightweight while ensuring structural strength by configuring the positioning convex point 2182 as a shell hollow nest, reduces the inertia of the moving part, and improves the response sensitivity; the hollow design can also enhance the elastic deformation ability of the spring sheet 2181, making it more flexible and smooth during locking and releasing, and further improving the operation convenience and overall reliability of assembly and disassembly.

[0092] The thickness of the fixed end root of the spring sheet 2181 is greater than that of the movable end, which can ensure that the spring sheet is firm enough while being flexible enough, perfectly balancing the structural strength and elasticity requirements, and greatly prolonging the service life of the spring sheet. The lower surface of the spring sheet 2181 near the movable end is a plane, which cooperates with the top of the clamping table 231 in the bracket 23; the contact area can be maximized to achieve stable locking.

[0093] In this embodiment, the height of all the suction head cabins 214 is set to penetrate the height of the support 211, and all the suction head cabins 214 are arranged side by side along the length of the support 211, so that the walls between the cabins together form an integral whole, enhancing the mechanical strength and rigidity of the suction head support, making it not easy to bend or break when the pipette pump plugs and unplugs the suction head, and ensuring the reliability of long-term use.

[0094] The top of the filter rod cabin 213 is lower than the top of the suction head cabin 214, but when the filter membrane pressure rod 216 is inserted into the filter rod cabin 213, it can be flush with the suction head cabin 214 with a suction head, which is not only convenient for processing, but also convenient for loading and placing. The top of the filter rod cabin 213 is higher than the height of the heating cabin 212, which can reserve sufficient operation space for the downward movement and pressure application of the filter membrane plate 32, preventing the filter membrane plate 32 from colliding with the heating cabin 212. In addition, the filter rod cabin 213 needs to accommodate the solid filter membrane pressure rod 216, so its height needs to be greater than the height of the rod body; the heating cabin 212 is mainly used to hold liquid for heating, and its height is determined by the required amount of liquid and the position of the heat source; it reflects the functional division of solid operation and liquid treatment.

[0095] The heights of the filter rod cabin 213 and the heating cabin 212 are both less than the height of the suction head cabin 214, and the lower bottom surface position of the filter rod cabin 213 is higher than that of the heating cabin 212. During the filtration operation, the filter membrane pressure rod 216 squeezes the liquid in the heating cabin 212 upward for filtration; if the bottom surface of the heating cabin 212 is lower, its liquid level height will naturally be lower than the bottom outlet of the filter rod cabin 213; the use of gravity ensures that the filtered liquid can be smoothly collected, effectively prevents backflow of the filtrate into the heating cabin, and ensures the accuracy and reliability of the test.

[0096] Preferably, the diameter of the filter rod cabin 213 is greater than the diameter of the heating cabin 212, and the diameter of the heating cabin 212 is greater than the diameter of the suction head cabin 214. The width of the support 211 in the position section where the heating cabin 212 is located is greater than the width of the support 211 in the position section where the suction head cabin 214 is located.

[0097] As shown in the drawings, at least two groups of grooves are further provided on both sides of the width of the support 211, and a positioning lock catch 217 is provided on each groove. The positioning lock catch 217 is configured as a boss structure extending horizontally and outwardly, and the lower bottom surface of the positioning lock catch 217 is flush with the lower bottom surface of the support 211. Figures 6-7

[0098] Further, the two end positioning lock catches 217 provided along the length direction of the support 211 are both arranged close to the position of the suction head cabin 214 at the outermost side of the two ends. Along the width direction of the support 211, the width of the outwardly extending positioning lock catch 217 is greater than the outer side of the support 211.

[0099] The suction head support 21 proposed in the embodiment realizes quick and accurate stable locking with the cradle 23 through the elastic lock catch 218 structure formed by the elastic sheet 2181 and the positioning boss 2182, is convenient and reliable to operate, optimizes the filter structure while ensuring the pipetting precision, improves the heating stability through the widened heating area structure, and ensures the overall stability in the movement of the cradle 23 in combination with the lateral positioning lock catch 217, thereby providing key consumable support for full-automatic and zero-pollution chemiluminescence detection.

[0100] As shown in the drawings, it is a reagent strip 22, which includes a strip body 221. A sample cabin 222, a plurality of component cabins 223, and a plurality of reaction cups 224 are provided on the strip body 221. The reaction cups 224 are arranged at intervals at one end of the strip body 221. All the component cabins 223 are interconnected and arranged in the middle of the strip body 221, and the sample cabin 222 is arranged at the other end of the strip body 221. A handle 225 is further provided at the end close to the sample cabin 222 for easy taking and placing. This is a common structure in the field, and will not be described in detail here. Figure 12 As shown in the drawings, the film pressing module 30 and the pipetting module 40 are arranged on the upper frame 11, and the film pressing module 30 is arranged close to one side of the suction head support 21. The film pressing module 30 and the pipetting module 40 are both arranged across the width direction of the upper frame 11.

[0101] Figures 13-14 As shown in the drawings, the film pressing module 30 and the pipetting module 40 are arranged on the upper frame 11, and the film pressing module 30 is arranged close to one side of the suction head support 21. The film pressing module 30 and the pipetting module 40 are both arranged across the width direction of the upper frame 11.

[0102] As shown in the drawings, the film pressing module 30 and the pipetting module 40 are arranged on the upper frame 11, and the film pressing module 30 is arranged close to one side of the suction head support 21. The film pressing module 30 and the pipetting module 40 are both arranged across the width direction of the upper frame 11. Figure 15 ​​As shown, the film pressing module 30 is mainly used for sample pretreatment, including a film pressing plate 32 and a film piercing needle 37 arranged on the film pressing plate; it also includes a fixed side plate 33 for fixing the film pressing plate 32, and a film pressing motor 31 for driving the film pressing plate 32, and a vertical film pressing guide rail is arranged on the fixed side plate 33, which cooperates with a film pressing slider 35 on the film pressing plate 32; the film pressing motor 31 drives the film pressing plate 32 to move vertically up and down along the film pressing guide rail through a film pressing lead screw 34, so as to control the film pressing plate 32 to insert the filter membrane pressing rod 216 into the heating cabin 212 to filter the liquid, or to pierce the cabin membrane on the reagent strip 22 through the film piercing needle 37 arranged on the film pressing plate 32.

[0103] As shown in Figure 16 , the fixed side plate 33 is vertically fixed on the upper layer plate in the upper layer frame 11, and the film pressing plate 32 is slidably connected to the film pressing guide rail on the fixed side plate 33 through the film pressing slider 35. The film pressing motor 31 is connected to the film pressing coupling 38 in the film pressing bearing seat 36 through the film pressing bearing seat 36, and is connected to the film pressing lead screw slider fixed on the film pressing plate 32 through the film pressing coupling 38. The film pressing motor 31 drives the film pressing lead screw 34 to rotate through the film pressing coupling 38, so as to drive the film pressing lead screw slider to reciprocate up and down on the film pressing lead screw 34, and then drive the film pressing plate 32 to move up and down.

[0104] Among them, the film pressing bearing seat 36 is fixed on the upper end face of the upper layer plate, and the fixed side plate 33 is fixed on the lower end face of the upper layer plate; and the whole is arranged above the filter rod cabin 213 in the suction head support 21. In order to reduce the overall weight of the side plate 33, the overall strength is guaranteed, and the fixed side plate 33 is designed as a U-shaped structure. Preferably, a plurality of grooves can be further constructed on the side of the fixed side plate 33 away from the film pressing plate 32, so as to further reduce the weight. In order to avoid the installation position of the film pressing lead screw 34, the middle of the film pressing plate 32 is hollow, which is designed as a "mouth" type structure, and in order to guarantee the consistency of the pressure of the lower bottom surface of the film pressing plate 32 against the filter membrane pressing rod 216, the lower bottom surface is a flat plane.

[0105] As shown in Figures 17-18 , at least one film piercing needle 37 is arranged on the side of the film pressing plate 32 away from the fixed side plate 33, all the film piercing needles 37 are arranged along the length direction of the film pressing plate 32, and the arrangement number and position of the film piercing needles 37 are consistent with the number and position of the reagent strips 22.

[0106] As shown in Figure 1 , Figure 13As shown, the pipetting module 40 is used for suction / drainage operation, suspended in the middle of the upper layer plate, in order to provide sufficient moving operation space for the pipetting module 40, a rectangular hollow position is arranged in the middle of the upper layer plate, which is consistent with the length and width of the upper layer plate, and the pipetting module 40 is suspended on the upper layer plate and arranged across the width direction.

[0107] As shown, the pipetting module 40 includes a row pipetting pump 42, a suction head guide sleeve 48, a pipetting frame 41 for fixing the row pipetting pump 42, a lifting motor 44 for driving the row pipetting pump 42 to move up and down, and a suction and drainage motor 43 for controlling the suction and drainage of the row pipetting pump 42. Figure 19

[0108] The two ends of the pipetting frame 41 in the width direction are provided with outwardly arranged U-shaped grooves, and a screw block 46 is installed in the U-shaped groove; on the upper end surface of the upper layer plate, a group of symmetrically arranged linear guide rails 45 and a pipetting screw are arranged along the length direction, wherein the pipetting screw is located above the linear guide rails 45, and the end away from the membrane pressing module 30 is connected with a screw motor 47. The lower bottom surface of the U-shaped groove is fixedly provided with a linear block 412 matched with the linear guide rails 45, and the screw motor 47 drives the pipetting screw to rotate to drive the screw block 46 to move along the linear guide rails 45 together with the pipetting frame 41 and the row pipetting pump 42.

[0109] A pipetting vertical plate 49 and a connecting plate 413 are arranged above the row pipetting pump 42, the pipetting vertical plate 49 is slidably connected with the vertical guide rail 410 on the pipetting frame 41 through a sliding block, and the row pipetting pump 42 is fixedly connected with the pipetting vertical plate 49. The position height of the pipetting frame 41 is unchanged, and it is always installed on the linear guide rails 45 of the upper layer plate through the screw block 46. The lower section of the pipetting vertical plate 49 is provided with a plurality of notches with different widths, which are cross-matched with the horizontal protrusions in the connecting plate 413; and the lower bottom surface of the connecting plate 413 is directly connected with the piston of the row pipetting pump 42.

[0110] The lifting motor 44 is fixed to the upper section of the other side wall surface of the pipetting frame 41 away from the row pipetting pump 42, which drives the lifting screw fixed to the side wall surface close to the row pipetting pump 42 to rotate through belt transmission, so as to drive the screw block fixed to the pipetting vertical plate 49 to drive the pipetting vertical plate 49, the connecting plate 413 and the row pipetting pump 42 to move up and down along the vertical guide rail 410.

[0111] ​The suction / discharge motor 43 is fixed to the upper end face of the pipetting vertical plate 49 via a bearing seat, and is connected to the screw slider fixed on the connecting plate 413 via a ball screw. The combined pipetting pump 42 includes 12 pump bodies, the upper end of the piston is directly connected to the lower end face of the connecting plate 413, and the lower end of the piston directly controls the pipetting pump head 411. A suction tip guide sleeve 48 is also fixed to the outside of the pipetting pump head 411.

[0112] like Figure 20 As shown, this is the state when the pipette pump head 411 picks up the pipette tip 215 / filter membrane clamp 216, at which time the pipette tip guide sleeve 48 is lifted. This occurs when the pipette pump head 411 needs to pick up the pipette tip 215 or filter membrane clamp 216. First, the entire pipetting module 40 can be controlled to move to the position of the pipette tip 215 or filter membrane clamp 216. Then, the lifting motor 44 controls the pipette pump head 411 to the preset position. Finally, the suction and discharge motor 43 drives the suction and discharge screw to drive the screw slider to drive the connecting plate 413 to push the piston on the linked pipette pump 42 to descend, directly inserting it into the top hole of the pipette tip 215 or filter membrane clamp 216. Then, the piston is controlled to move upward, that is, the pipette pump head 411 drives the pipette tip 215 or filter membrane clamp 216 to detach from its chamber, completing the removal work.

[0113] like Figure 21 As shown, this is the free state when the pipette tip 215 or filter membrane lever 216 needs to be removed. At this time, the pipette tip retraction guide sleeve 48 falls down, and the pipette pump head 411 is embedded in the pipette tip retraction guide sleeve 48. Specifically, the connecting plate 413 is driven by the suction / discharge motor 43 to drive the piston to rise. The piston drives the pipette tip 215 or filter membrane lever 216 to move upward together through the pipette pump head 411. When the position of the pipette pump head 411 is lower than the bottom surface of the pipette tip retraction guide sleeve 48, the pipette tip retraction guide sleeve 48 can prevent the pipette tip 215 or filter membrane lever 216 from moving upward, thus detaching the pipette tip 215 or filter membrane lever 216 from the pipette pump head 411.

[0114] like Figure 22 As shown in the diagram, the dashed line representing the pipette tip 215 indicates the movement path when liquid needs to be aspirated or drained from a designated compartment. The dashed line indicates the lowest position reached by the pipette tip 215. During aspiration, the entire pipetting module 40 is first moved to the corresponding position by the lead screw motor 47. Then, the lifting motor 44 controls the pipetting pump head 411 with the pipette tip 215 to move downwards until the pipette tip 215 is inserted into the compartment. Next, the suction / drainage motor 43 drives the connecting plate 413 to raise the piston, and the connecting pipetting pump 42 controls the pipette tip 215 to aspirate liquid. During draining, the lifting motor 44 first moves the pipette tip 215 upwards, then the lead screw motor 47 moves it horizontally to the designated compartment in the reagent strip 22. Finally, the suction / drainage motor 43 drives the connecting plate 413 to lower the piston, draining the liquid from the pipette tip 215 into the corresponding compartment, thus completing the draining process.

[0115] As Figure 1 shown, on the lower layer plate in the lower layer frame 12, a heating module 50 for high-temperature heating of samples in the sample chamber 222 of the reagent strip 22, a magnetic separation module 60 for separating reagents, and an optical acquisition module 70 for optically acquiring information are installed, wherein the heating module 50 is arranged close to the material preparation module 20 side, the optical acquisition module 70 is located at the tail of the lower layer plate, and the magnetic separation module 60 is located between the heating module 50 and the optical acquisition module 70.

[0116] By using a plurality of groups of independently controlled side-by-side pipetting pumps 42 cooperating with disposable pipette tips 215 to perform sample addition and reaction, the sample addition and reaction process of each sample is completely independent, physically isolating any contact between different samples, thereby fundamentally ensuring the absolute accuracy of the detection result, especially meeting the high requirement project of zero tolerance to cross contamination of fungus detection, and fundamentally solving the problem of sample contamination carried between samples inherent in the traditional steel needle sample addition system.

[0117] As Figure 23 shown, the heating module 50 includes a base plate 51, a heating motor 52 fixed on the right side of the base plate 51, a heating unit 56 arranged on the left side of the base plate 51, and a support plate 53 for fixing the heating unit 56. The heating unit 56 is a conventional heating assembly, which is well known and will not be described in detail here. A vertical guide rail two 55 is provided on the side wall of the base plate 51 close to the heating unit 56, which is connected with the heating unit 56 through a sliding block. A cam disc 54 is provided on the left side wall of the base plate 51 and connected with the output end of the heating motor 52. A long circular hole is provided on the support plate 53, and a cam shaft is provided on the cam disc 54 and matched with the long circular hole. The heating motor 52 rotates through the cam disc 54, drives the support plate 53 to rise and fall through the cam shaft, and drives the heating unit 56 to move vertically up and down.

[0118] As Figures 24-25 shown, only one set of lifting mechanism composed of the support plate 53, the cam disc 54 and the vertical guide rail two 55 in the heating module 50, which only lifts the heating unit 56. When the cam disc 54 is rotated by 180° by the heating motor 52 (into the drawing), the heating unit 56 is lifted to the highest position, and the heating unit 56 is in the highest position. Figure 24As indicated by the rotating arrow, the support plate 53 is lifted by the camshaft, which in turn lifts the heating unit 56 to its highest position and then remains stationary. The heating cup in the raised heating unit 56 then fits onto the heating chamber 212 in the suction head bracket 21, where it begins high-temperature heating. After heating is complete, the heating motor 52 controls the cam disk 54 to rotate in the opposite direction, causing the support plate 53 to move downwards along the vertical guide rail 25 until it stops at its initial position. Using the cam disk 54 to drive the lifting and lowering of the heating unit 56 results in a simple structure, reliable transmission, and eliminates the need for complex pneumatic or hydraulic systems, reducing manufacturing costs and facilitating maintenance. It also ensures a tight fit between the heating unit 56 and the heating chamber 212, improving heating efficiency and temperature uniformity.

[0119] The magnetic separation module 60 is used to quickly and effectively separate antigen-antibody complexes and impurities that are bound to magnetic particles. Its structure is existing technology and will not be described in detail here.

[0120] like Figures 26-27 As shown, the optical acquisition module 70 includes an acquisition stepper motor 71, a linear guide rail 72 spanning the width of the lower layer plate, a detection slide 73, a photon counter 74, and a darkroom column 75 fixed on the linear guide rail 72. The linear guide rail 72 is horizontally fixed to the support frame of the lower frame 12. The acquisition stepper motor 71 is fixed to one end of the linear guide rail 72, positioned near the external interface 16. The darkroom column 75 is located on the same side of the linear guide rail 72 as the acquisition stepper motor 71, and is positioned at opposite ends of the linear guide rail 72. The photon counter 74 is mounted on the detection slide 73 and can move with the detection slide 73. A synchronous belt is provided on the back of the linear guide rail 72, and the acquisition stepper motor 71 drives the detection slide 73 and the photon counter 74 to slide left and right along the linear guide rail 72 via the belt transmission.

[0121] like Figure 28As shown, the detection slide 73 includes a mounting table 734, a detection stepping motor 731, two sets of symmetrically arranged vertical guide rails three 732, and a telescopic sleeve 733 in sliding fit with the vertical guide rails three 732. Among them, the photon counter 74 is provided with a code scanner 735 on the side close to the collection stepping motor 71, the code scanner 735 is fixedly installed on the mounting table 734, and the detection stepping motor 731 is vertically fixed on the mounting table 734, the lead screw of which passes through the mounting table 734 and is movably connected with the telescopic sleeve 733 through a lead screw block. The telescopic sleeve 733 includes side edge plates in sliding fit with the vertical guide rails three 732, and a front plate on which a laser detection head 736 is mounted, and the side edge plates are also directly fixedly connected with the lead screw block of the detection stepping motor 731. The back of the detection stepping motor 731 is also provided with a circuit board 76 for monitoring the overall work of the detection slide 73. The darkroom column 75 completely wraps the laser detection head 736 during the non-detection period, avoiding environmental light interference and physical damage, and prolonging the service life of the device; during detection, the detection stepping motor 731 precisely controls the lifting and horizontal movement to ensure that the photon counter is aligned with the reaction cup, thereby improving the detection accuracy and repeatability.

[0122] In the initial position, the detection slide 73 is located at one end of the darkroom column 75 and the laser detection head 736 is deeply buried in the darkroom column 75 to protect the laser detection head 736. When it is necessary to detect the reaction cup on the reagent strip 22, the detection stepping motor 731 first controls the telescopic sleeve 733 to move upwards, so that the laser detection head 736 first leaves the darkroom column 75; then the detection slide 73 is moved away from the darkroom column 75 by the collection stepping motor 71 until it moves above the corresponding reaction cup in the corresponding reagent strip 22. Then the detection stepping motor 731 drives the telescopic sleeve 733 to press down through the vertical guide rails three 732, and then drives the laser detection head 736 to insert into the reaction cup, the number of photons is read by the photomultiplier tube, and then the detection of the reagent is completed; the detection result is transmitted to the main control module 90 through the circuit board 76, and the result is displayed on the display screen of the shell 10 under the control of the main control module 90. The code scanner 735 is used for scanning the label on the reagent strip 22 to obtain the information to be detected.

[0123] In work, first open the hatch 13 of the shell 10, and then place 12 five-in-one test reagent strips 22 and 12 suction head supports 21 on the bracket 23 in sequence, each suction head support corresponding to a reagent strip; and the system can automatically judge the situation of placing more or less suction head supports and reagent strips. The bracket 23 and the five-in-one test reagent strips 22 and suction head supports 21 thereon are driven to move along the linear guide rail one 25 to the heating module 50, the magnetic separation module 60 or the optical collection module 70 for combined detection.

[0124] Before the joint detection, the sample in the reagent strip needs to be added for sample processing, that is, the treated sample is added to the corresponding reaction cup in the five joint detection reagent strip, and the corresponding reagent components are added. The heating chamber 212 in the suction head support 21 is bath heated by the high temperature heating module 50, that is, the process of combining an enzyme-labeled substance with an antibody by using a chemiluminescence immunoassay method, which usually takes 10 minutes. Based on the experimental process requirements, the magnetic separation module 60 is used to perform multiple magnetic separation and cleaning operations on the sample chamber 222 in the reagent strip; at the same time, AB liquid is also added to the sample chamber to make the liquid in the sample chamber produce excitation light; finally, the optical acquisition module is used to analyze and test the solution in the sample chamber, and finally the detection result required is obtained. Correspondingly, the detected value is directly displayed on the display screen on the surface of the shell 10.

[0125] The analyzer supports simultaneous joint detection of fungal markers and multiple inflammation indicators, and multiple related indicator results can be obtained through one detection, providing more accurate and comprehensive disease evaluation basis, effectively solving the early diagnosis difficulty problem caused by single indicator detection and clinical performance overlap, and improving the diagnosis and treatment efficiency. It has the characteristics of simple structure, convenient operation, high reliability and low maintenance requirement, and its detection flux precisely meets the daily detection needs of small and medium-sized hospitals and primary medical institutions, accurately meets the clinical joint detection needs, and reduces the manufacturing cost through structural simplification, with very high cost performance.

[0126] The chemiluminescence immunoassay analyzer designed in the application can perform zero-pollution operation on multiple reagent strips through the use of independent pipette joint pump and disposable suction head without liquid path design. Compared with the traditional liquid path system, the maintenance cost is reduced by more than 60%, and the equipment reliability is significantly improved. The modular layered structure design reduces the instrument volume to less than 0.5m³, which is suitable for desktop deployment in primary hospitals. At the same time, it supports five joint detection of invasive fungi and inflammation indicators, and can simultaneously complete multiple indicator analysis in a single detection, with a detection time reduced by more than 50%. The technical scheme realizes the miniaturization, simple operation and low maintenance cost of the equipment while ensuring the detection accuracy, and is particularly suitable for secondary hospitals and community hospitals.

[0127] The embodiments of the application are described in detail above, and the content is only the preferred embodiment of the application, which cannot be considered as limiting the scope of the application. Any equivalent changes and improvements made within the scope of the application should still belong to the patent coverage range of the application.

Claims

1. A chemiluminescence immunoassay analyzer, characterized in that, At least including: The material preparation module is used to place one or more pipette tip holders and reagent strips; the material preparation module also includes a bracket that supports the pipette tip holders and reagent strips, a gantry bracket that fixes the bracket, and a lead screw motor that drives the gantry bracket to slide relative to a linear guide rail, the lead screw motor driving the bracket to move along the length of the bracket through a lead screw slider. A membrane pressing module for sample pretreatment includes a membrane pressing plate and a membrane-piercing needle disposed on the membrane pressing plate. The membrane pressing module and the pipetting module are mounted on an upper frame, with the membrane pressing module positioned closer to the pipette tip holder. Both the membrane pressing module and the pipetting module are arranged across the width of the upper frame. The membrane pressing module further includes a fixed side plate for fixing the membrane pressing plate and a membrane pressing motor for driving the membrane pressing plate. A membrane pressing guide rail is provided on the fixed side plate, which cooperates with a membrane pressing slider on the membrane pressing plate. The membrane pressing motor drives the membrane pressing plate to move vertically up and down along the membrane pressing guide rail via a membrane pressing screw. The membrane pressing plate is positioned directly above the filter compartment in the pipette tip holder. At least one membrane-piercing needle is provided on the side of the membrane pressing plate away from the fixed side plate, and the membrane-piercing needle is arranged along the length of the membrane pressing plate, with the position of the membrane-piercing needle adapted to the arrangement of the reagent strips. A pipetting module for aspiration / dispensing operations includes a series pipetting pump and a pipette tip guide sleeve. The module also includes a pipetting frame for fixing the series pipetting pump, a lifting motor for driving the series pipetting pump to move up and down, and a suction / dispensing motor for controlling the aspiration and dispensing of the series pipetting pump. The series pipetting pump is fixed to the outer wall of the pipetting frame. Above the series pipetting pump, a cross-shaped pipetting vertical plate and a connecting plate are provided. The pipetting vertical plate is connected to a vertical slide rail on the pipetting frame via a slider. The lifting motor drives a lifting screw via a belt drive to drive a slider on the pipetting vertical plate, causing the pipetting vertical plate and the series pipetting pump to move up and down synchronously. The suction / dispensing motor is fixed to the pipetting vertical plate and drives a slider on the connecting plate via a suction / dispensing screw, causing the connecting plate to move away from / push the piston on the series pipetting pump to rise / fall, so that the pipette tip aspirates / dispenses liquid. The combined pipette pump is controlled to drive the tip retraction guide sleeve, so that the filter membrane rod can be removed from the tip holder and placed in the filter rod chamber, or the tip can be removed and liquid aspiration / discharge operations can be performed. The pressure plate is controlled to press the filter membrane rod into the filter rod chamber, or the membrane is opened in each chamber of the reagent strip by the puncture needle.

2. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The bracket has opposing guide wheel grooves on its back side, and at least two sets of guide wheel brackets cooperate with the guide wheel grooves to provide fixed support when the bracket slides back and forth.

3. The chemiluminescence immunoassay analyzer according to claim 1, characterized in that, The pipette rack has outward-facing U-shaped grooves at both ends in the width direction. A lead screw slider is installed in the U-shaped groove. A linear slider is provided on the bottom surface of the U-shaped groove to cooperate with the linear guide rail. The lead screw motor drives the pipette lead screw to rotate so that the lead screw slider, the pipette rack and the connected pipette pump move back and forth along the linear guide rail.

4. A chemiluminescence immunoassay analyzer according to any one of claims 1-3, characterized in that, The material preparation module is configured on the lower frame, which also includes a heating module for heating the sample in the reagent strip at high temperature, a magnetic separation module for separating reagents, and an optical acquisition module for optical information acquisition. The heating module is located close to the material preparation module, and the magnetic separation module is located between the heating module and the optical acquisition module.

5. A chemiluminescence immunoassay analyzer according to claim 4, characterized in that, The heating module includes a base plate, a heating motor, a heating unit, and a support plate for fixing the heating unit; a vertical guide rail is provided on the base plate, which is slidably connected to the heating unit; the heating motor drives the support plate to rise and fall through a cam plate, so as to drive the heating unit to move vertically up and down.

6. A chemiluminescence immunoassay analyzer according to claim 5, characterized in that, The optical acquisition module includes an acquisition stepper motor, a linear guide rail three, a detection slide, and a photon counter. The linear guide rail three is horizontally fixed on the lower frame, and the acquisition stepper motor is fixed on the linear guide rail three and drives the detection slide to slide along the linear guide rail three via belt transmission.

7. A chemiluminescence immunoassay analyzer according to any one of claims 1-3 and 5-6, characterized in that, It also has a shell with a door at the head corresponding to the material preparation module, an air outlet at the back, an air inlet at the bottom, and an external interface on one side wall; a power module and a main control module are also configured on the frame inside the shell.

Images