Anti-wrinkle face cream as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to anti-wrinkle face cream as well as a preparation method and application thereof. The anti-wrinkle face cream comprises an anti-wrinkle agent composition and a penetration enhancer, the anti-wrinkle agent composition is prepared from beta-glucan, ubiquinone, hydroxypropyl tetrahydropyrantriol, adenosine, a ginseng root extract, acetyl hexapeptide and a substance A; the substance A comprises soluble collagen; the penetration enhancer comprises lecithin. The penetration enhancer lecithin and the anti-wrinkle agents beta-glucan, ubiquinone, hydroxypropyl tetrahydropyrantriol, adenosine, ginseng root extract, acetyl hexapeptide and soluble collagen are compounded for use, all the components have a synergistic effect, the anti-wrinkle effect can be remarkably improved, and the anti-wrinkle effect can be improved by combining other components. The face cream is high in elastase inhibition rate, high in DPPH free radical scavenging rate and good in anti-wrinkle effect.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle cream as well as a preparation method and application thereof. BACKGROUND

[0002] Wrinkle formation is closely related to free radical damage and collagen degradation. Free radicals destroy skin cell membranes and collagen through oxidation, leading to skin relaxation and fine lines, which are commonly seen around the eyes, forehead, neck and other parts. With the acceleration of life pace and the increase of environmental pressure, the demand for anti-wrinkle products continues to grow. Consumers are pursuing the improvement of skin relaxation and fine line problems from the root, and there is an urgent need for high-efficiency, safe and long-acting anti-wrinkle products.

[0003] At present, existing anti-wrinkle products, such as anti-wrinkle creams, have single components, which makes the anti-wrinkle effect unsatisfactory, such as low elastase inhibition rate and low free radical scavenging rate, and the anti-wrinkle effect needs to be further improved.

[0004] Therefore, it is of great significance to provide a cream with high elastase inhibition rate and high free radical scavenging rate, and further with good anti-wrinkle effect. SUMMARY

[0005] The present application aims to solve one or more technical problems existing in the prior art, and at least provide a beneficial choice. Specifically, the present application provides an anti-wrinkle cream with high elastase inhibition rate, high free radical scavenging rate and good anti-wrinkle effect.

[0006] The inventive concept of the present application: the anti-wrinkle cream of the present application comprises an anti-wrinkle agent composition and a penetration enhancer; the anti-wrinkle agent composition comprises beta-glucan, ubiquinone, hydroxypropyl tetrahydropyrane triol, adenosine, ginseng root extract, acetyl hexapeptide and substance A; the substance A comprises soluble collagen; and the penetration enhancer comprises lecithin.

[0007] The present application uses lecithin as a penetration enhancer and beta-glucan, ubiquinone, hydroxypropyl tetrahydropyrane triol, adenosine, ginseng root extract, acetyl hexapeptide and soluble collagen in the anti-wrinkle agent composition, and there is a synergistic effect between the components, which can significantly improve the anti-wrinkle effect. In combination with other components, the cream of the present application has high elastase inhibition rate, high 1,1-diphenyl-2-trinitrobenzene hydrazine radical (DPPH) scavenging rate and good anti-wrinkle effect.

[0008] Therefore, the first aspect of the present application provides an anti-wrinkle cream.

[0009] Specifically, the anti-wrinkle cream comprises an anti-wrinkle agent composition and a penetration enhancer.

[0010] The anti-wrinkle agent composition comprises β-glucan, ubiquinone, hydroxypropyl tetrahydropyrane triol, adenosine, ginseng root extract, acetyl hexapeptide, substance A;

[0011] The substance A comprises soluble collagen;

[0012] The penetration enhancer comprises lecithin.

[0013] Preferably, the acetyl hexapeptide comprises acetyl hexapeptide-8.

[0014] Specifically, the face cream of the present application has high anti-wrinkle effect through the specific type of penetration enhancer and the synergistic effect of each component in the anti-wrinkle agent composition.

[0015] Lecithin: Lecithin molecules have both hydrophilic and hydrophobic properties, which makes them able to form a stable interface on the skin surface, thus promoting the penetration of active ingredients through the skin barrier and improving the absorption rate. At the same time, lecithin can act as an emulsifying agent, stabilizing the oil-water phase and forming a uniform emulsion or liposome structure. This structure helps to encapsulate and deliver active ingredients to the deep layers of the skin, enhancing their penetration effect.

[0016] Adenosine: Adenosine can stimulate fibroblasts to produce collagen, increase skin elasticity, and reduce wrinkles. Its antioxidant properties can neutralize free radicals, delay skin aging, and inhibit collagenase activity, maintaining collagen content.

[0017] β-glucan: β-glucan can inhibit the activity of interstitial collagenase (MMP-1) and gelatinase B (MMP-9), promote the synthesis of type I collagen, reduce wrinkle depth, scavenge free radicals, upregulate the Nrf2 / HO-1 pathway, enhance antioxidant enzyme activity, and protect the skin from oxidative damage.

[0018] Ubiquinone: Ubiquinone can delay skin aging by neutralizing free radicals, reducing oxidative stress, and protecting cells from damage. Its antioxidant effect can enhance skin repair ability, reduce wrinkle depth, improve skin dullness, and provide protection against epidermal damage caused by UVA radiation. By inhibiting collagenase activity, ubiquinone helps to promote collagen proliferation, enhance skin elasticity, and reduce fine lines and laxity.

[0019] Hydroxypropyl tetrahydropyrane triol: Hydroxypropyl tetrahydropyrane triol stimulates fibroblasts to produce type IV and type VII collagen, enhances dermal layer elasticity, improves fine lines and wrinkles, and enhances skin firmness. It promotes the synthesis of intercellular junction proteins such as integrins and laminins, strengthens the connection between the epidermis and the dermis, reduces water loss, and enhances skin resistance.

[0020] Ginseng Root Extract: Ginseng Root Extract delays skin aging, reduces wrinkles and fine lines (such as crow's feet) by scavenging free radicals and inhibiting melanin production. It contains active ingredients such as ginsenosides, which can neutralize free radicals, protect the skin from environmental damage, promote cell repair and regeneration, and make the skin tighter and smoother.

[0021] Acetyl Hexapeptide: Acetyl Hexapeptide effectively smooths dynamic lines (such as expression lines) and static lines by interfering with the release of neurotransmitter acetylcholine through competition for the N-terminal site of SNAP-25 protein, reducing facial muscle overcontraction. At the same time, it can also activate elastin activity, stimulate collagen synthesis, increase skin elasticity, and improve sagging, making the skin tighter and smoother.

[0022] Soluble Collagen: Soluble Collagen delays skin aging, enhances skin elasticity, and reduces wrinkles by supplementing the lost collagen in the body. Its molecular structure is highly consistent with human collagen, which can promote cell autologous collagen secretion, achieving the anti-aging effect of external and internal supplementation.

[0023] The above-mentioned specific types of lecithin, beta-glucan, ubiquinone, hydroxypropyl tetrahydro pyran triol, adenosine, ginseng root extract, acetyl hexapeptide, and soluble collagen are compounded and synergistically act to significantly improve the anti-wrinkle effect of the cream.

[0024] Preferably, the anti-wrinkle cream also includes a humectant, an emollient, a skin conditioner, an antioxidant, a thickening agent, a chelating agent, an emulsifier, and water.

[0025] Preferably, the humectant includes at least one of glycerin, sodium hyaluronate, inositol, butylene glycol, a mixture consisting of xylitol-based glucoside, anhydrous xylitol, xylitol, and water, and 1,2-hexanediol.

[0026] Further preferably, the humectant includes glycerin, sodium hyaluronate, inositol, butylene glycol, a mixture consisting of xylitol-based glucoside, anhydrous xylitol, xylitol, and water, and 1,2-hexanediol.

[0027] Preferably, the emollient includes at least one of caprylic / capric triglyceride, dimethicone, shea butter, a mixture consisting of phytosteryl oleate and phytosterols, and jojoba seed oil.

[0028] Further preferably, the emollient includes caprylic / capric triglyceride, dimethicone, shea butter, a mixture consisting of phytosteryl oleate and phytosterols, and jojoba seed oil.

[0029] Preferably, the skin conditioner includes at least one of dipotassium glycyrrhizate, a mixture consisting of water, butylene glycol, and purslane extract, and white camomile flower oil.

[0030] Further preferably, the skin conditioning agent comprises dipotassium glycyrrhizate, chamomile flower oil, a mixture consisting of water, butylene glycol, and purslane extract.

[0031] Preferably, the antioxidant comprises at least one of tocopheryl acetate, tetrahydro methyl pyrimidine carboxylic acid, and para-hydroxyacetophenone.

[0032] Further preferably, the antioxidant comprises tocopheryl acetate, tetrahydro methyl pyrimidine carboxylic acid, and para-hydroxyacetophenone.

[0033] Preferably, the thickening agent comprises at least one of a mixture consisting of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate, sorbitan isostearate, and stearyl alcohol.

[0034] Further preferably, the thickening agent comprises a mixture consisting of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate, sorbitan isostearate, and stearyl alcohol.

[0035] Preferably, the polysorbate comprises polysorbate-60.

[0036] Preferably, in the thickening agent, the weight ratio of the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, the polysorbate, and the sorbitan isostearate is 45:(0.8-1.2):(0.8-1.2); further preferably, the weight ratio of the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, the polysorbate, and the sorbitan isostearate is 45:(0.9-1.1):(0.9-1.1); and more further preferably, the weight ratio of the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, the polysorbate, and the sorbitan isostearate is 45:1:1.

[0037] Preferably, in the thickening agent, the weight ratio of the total weight of the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, the polysorbate, and the sorbitan isostearate to the weight of the stearyl alcohol is (0.1-2):(0.1-5).

[0038] Preferably, the chelating agent comprises ethylenediaminetetraacetic acid disodium salt (EDTA disodium).

[0039] Preferably, the emulsifier comprises at least one of cetearyl olivate, sorbitan olivate.

[0040] Further preferably, the emulsifier comprises a mixture of cetearyl olivate and sorbitan olivate.

[0041] Preferably, the weight ratio of the cetyl stearyl olivate, sorbitan olivate in the emulsifier is (2.5-3.5):2; further preferably, the weight ratio of the cetyl stearyl olivate, sorbitan olivate is (2.7-3.3):2; more further preferably, the weight ratio of the cetyl stearyl olivate, sorbitan olivate is 3:2.

[0042] Preferably, the anti-wrinkle cream consists of the anti-wrinkle agent composition 0.11-16%, the penetration enhancer 0.1-5%, the humectant 1.3-34%, the emollient 1.2-24%, the skin conditioning agent 0.010-6.5%, the antioxidant 0.18-6%, the thickening agent 0.2-7%, the chelating agent 0.001-0.3%, the emulsifier 0.1-5% and the balance of water, by weight percentage.

[0043] Further preferably, the anti-wrinkle cream consists of the anti-wrinkle agent composition 0.12202-14.95%, the penetration enhancer 0.1-5%, the humectant 1.401-34%, the emollient 1.31-24%, the skin conditioning agent 0.012-6.5%, the antioxidant 0.21-6%, the thickening agent 0.2-7%, the chelating agent 0.001-0.3%, the emulsifier 0.1-5% and the balance of water, by weight percentage.

[0044] Preferably, the anti-wrinkle agent composition comprises the β-glucan 0.001-0.5%, the ubiquinone 0.001-0.3%, the hydroxypropyl tetrahydropyrane triol 0.1-10%, the adenosine 0.01-2%, the ginseng root extract 0.01-2%, the acetyl hexapeptide 0.00001-0.05%, the substance A 0.00001-0.1%, by weight percentage.

[0045] Further preferably, the anti-wrinkle agent composition comprises the β-glucan 0.05%, the ubiquinone 0.03%, the hydroxypropyl tetrahydropyrane triol 1%, the adenosine 0.5%, the ginseng root extract 0.2%, the acetyl hexapeptide 0.0005%, the substance A 0.0001%, by weight percentage.

[0046] Specifically, the weight percentage of each component in the anti-wrinkle agent composition is based on the total weight of the cream.

[0047] Preferably, the substance A further comprises the sodium chloride and the water.

[0048] Preferably, said substance A comprises soluble collagen, sodium chloride and water in a weight ratio of 8: (0.8-1.2): (0.8-1.2); further preferably, said soluble collagen, sodium chloride and water are in a weight ratio of 8: (0.9-1.1): (0.9-1.1); still further preferably, said soluble collagen, sodium chloride and water are in a weight ratio of 8:1:1.

[0049] Preferably, said penetration enhancer further comprises water; said penetration enhancer comprising lecithin and water in a weight ratio of 99.4: (0.5-0.7); further preferably, said penetration enhancer comprising lecithin and water in a weight ratio of 99.4: (0.55-0.65); still further preferably, said penetration enhancer comprising lecithin and water in a weight ratio of 99.4:0.6.

[0050] Preferably, said moisturizing agent comprises glycerin 1-10%, sodium hyaluronate 0.001-1%, inositol 0.1-3%, butylene glycol 0.1-10%, 1,2-hexanediol 0.1-5%, a mixture consisting of xylitol-based glucoside, anhydrous xylitol, xylitol and water 0.1-2% by weight percentage.

[0051] In particular, the weight percentage of each component in said moisturizing agent is based on the total weight of the face cream.

[0052] Preferably, in the mixture consisting of xylitol-based glucoside, anhydrous xylitol, xylitol and water, the weight ratio of said xylitol-based glucoside, anhydrous xylitol, xylitol and water is 8: (5-7): (2.5-3.5): (2.5-3.5); further preferably, the weight ratio of said xylitol-based glucoside, anhydrous xylitol, xylitol and water is 8: (5.5-6.5): (2.7-3.3): (2.7-3.3); still further preferably, the weight ratio of said xylitol-based glucoside, anhydrous xylitol, xylitol and water is 8:6:3:3.

[0053] Preferably, said emollient comprises caprylic / capric triglyceride 1-8%, dimethicone 0.1-5%, shea butter 0.1-5%, jojoba seed oil 0.01-1%, a mixture consisting of phytosteryl oleate and phytosterols 0.1-5% by weight percentage.

[0054] In particular, the weight percentage of each component in said emollient is based on the total weight of the face cream.

[0055] Preferably, in the mixture consisting of phytosteryl oleate and phytosterols, the mass ratio of phytosteryl oleate, phytosterols is 97: (2.5-3.5); further preferably, the mass ratio of phytosteryl oleate, phytosterols is 97: (2.7-3.3); more further preferably, the mass ratio of phytosteryl oleate, phytosterols is 97:3.

[0056] Preferably, the skin conditioning agent comprises, by weight percent, dipotassium glycyrrhizinate 0.001-0.5%, chamomile flower oil 0.001-1%, and a mixture consisting of water, butylene glycol, and purslane extract 0.01-5%.

[0057] In particular, the weight percent of each component in the skin conditioning agent is based on the total weight of the face cream.

[0058] Preferably, in the mixture consisting of water, butylene glycol, and purslane extract, the weight ratio of water, butylene glycol, and purslane extract is 69.4: (25-35): (0.5-0.7); further preferably, the weight ratio of water, butylene glycol, and purslane extract is 69.4: (27-33): (0.55-0.65); more further preferably, the weight ratio of water, butylene glycol, and purslane extract is 69.4:30:0.6.

[0059] Preferably, the antioxidant comprises, by weight percent, tocopheryl acetate 0.1-3%, tetrahydro methyl pyrimidine carboxylic acid 0.01-2%, and para-hydroxyacetophenone 0.1-1%.

[0060] In particular, the weight percent of each component in the antioxidant is based on the total weight of the face cream.

[0061] Preferably, the anti-wrinkle face cream comprises, by weight percent, a mixture consisting of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate, sorbitan isostearate 0.1-2%, disodium EDTA 0.001-0.3%, stearyl alcohol 0.1-5%, a mixture consisting of cetearyl alcohol and sorbitan olivate 0.1-5%, humectant 1.3-34%, emollient 1.2-24%, skin conditioning agent 0.010-6.5%, antioxidant 0.18-6%, penetration enhancer 0.1-5%, anti-wrinkle agent composition 0.11-16%, and the balance of water.

[0062] Further preferably, the anti-wrinkle cream consists of, in percentage by weight, a mixture of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate, sorbitan isostearate 0.1-2%, disodium EDTA 0.001-0.3%, stearyl alcohol 0.1-5%, a mixture of cetearyl olivate and sorbitan olivate 0.1-5%, humectant 1.401-34%, emollient 1.31-24%, skin conditioning agent 0.012-6.5%, antioxidant 0.21-6%, penetration enhancer 0.1-5%, anti-wrinkle agent composition 0.12202-14.95%, and the balance of water.

[0063] Further preferably, the anti-wrinkle cream consists of, in percentage by weight, a mixture of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate, sorbitan isostearate 0.1-2%, disodium EDTA 0.001-0.3%, stearyl alcohol 0.1-5%, a mixture of cetearyl olivate and sorbitan olivate 0.1-5%, humectant 1.401-34%, emollient 1.31-24%, skin conditioning agent 0.012-6.5%, antioxidant 0.21-6%, penetration enhancer 0.1-5%, anti-wrinkle agent composition 0.12202-14.95%, and the balance of water.

[0064] The second aspect of the present application provides a preparation method of the anti-wrinkle cream according to the first aspect of the present application.

[0065] Specifically, the preparation method of the anti-wrinkle cream comprises the following steps:

[0066] Mixing the components to obtain the anti-wrinkle cream.

[0067] Preferably, the preparation method of the anti-wrinkle cream comprises the following steps:

[0068] (1) mixing water, thickening agent, chelating agent, humectant, penetration enhancer, heating, and keeping warm to obtain mixture 1;

[0069] (2) mixing the emulsifier and the emollient, heating, then mixing the mixture 1, the antioxidant, to obtain mixture 2; after cooling, mixing the anti-wrinkle agent composition and the skin conditioning agent, to obtain the cream.

[0070] Preferably, in step (1), the heating temperature is 75-90℃; further preferably, the heating temperature is 80-85℃.

[0071] Preferably, in step (1), stirring is performed during the holding; the stirring time is 25-35min; further preferably, the stirring time is 27-33min; more preferably, the stirring time is 30min.

[0072] Preferably, the stirring is constant temperature, i.e. constant temperature stirring.

[0073] Preferably, in step (1), after the holding, homogenization is performed to make the powder evenly dispersed.

[0074] Preferably, in step (2), the heating temperature is 75-90℃; further preferably, the heating temperature is 80-85℃.

[0075] Preferably, in step (2), after mixing the mixture 1 and the antioxidant, homogenization is performed; further preferably, after mixing the mixture 1 and the antioxidant, homogenization is performed, then vacuuming and defoaming, and then cooling.

[0076] Preferably, the cooling is to 55-70℃; further preferably, the cooling is to 60-65℃.

[0077] Preferably, in step (2), after the cooling, the anti-wrinkle agent composition is first added, then the cooling is continued, and then the skin conditioning agent is added; further preferably, after the cooling, the anti-wrinkle agent composition is first added, then stirring is performed, then the cooling is continued, and then the skin conditioning agent is added.

[0078] Preferably, the cooling is continued to 37-50℃; further preferably, the cooling is continued to 40-45℃.

[0079] Preferably, the mixture of the hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, the polysorbate, and the sorbitan isosuccinate in the thickening agent is mixed in step (1); the stearyl alcohol in the thickening agent is mixed with the emulsifier and the emollient in step (2).

[0080] Preferably, the method for preparing the anti-wrinkle cream comprises the following steps:

[0081] (1) add water, a mixture composed of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-60, sorbitan isostearate, chelating agent, humectant, penetration enhancer into an emulsification kettle, heat and stir, heat to 75-90 DEG C, constant temperature stirring, then homogenize until the material is uniformly dispersed;

[0082] (2) add stearyl alcohol, emulsifier, emollient into an oil kettle, heat to 75-90 DEG C, pump into the emulsification kettle, vacuum homogenize until the material is uniform, add antioxidant, continue to homogenize until the material is uniform, start to cool after vacuum defoaming;

[0083] (3) cool to 55-70 DEG C, add anti-wrinkle composition, stir until the material is uniform;

[0084] (4) continue to cool to 37-50 DEG C, add skin conditioner, stir until the material is uniform; the anti-wrinkle cream is prepared.

[0085] The third aspect of the present application provides an anti-wrinkle cream according to the first aspect of the present application in the field of skin care products.

[0086] Compared with the prior art, the technical scheme provided by the present application has the following beneficial effects:

[0087] (1) The present application uses penetration enhancer lecithin and anti-wrinkle agent composition β-glucan, ubiquinone, hydroxypropyl tetrahydropyran triol, adenosine, ginseng root extract, acetyl hexapeptide, soluble collagen in combination, and there is a synergistic effect between the components, which can significantly improve the anti-wrinkle effect, and in combination with other components, the present application has high elastase inhibition rate and high DPPH free radical scavenging rate, the DPPH free radical scavenging rate can reach 28.37%, and the elastase inhibition rate can reach 28.49%, and has good anti-wrinkle effect.

[0088] (2) The preparation process of the present application is simple and convenient for large-scale production and application. DETAILED DESCRIPTION

[0089] In order to make those skilled in the art more clearly understand the technical scheme of the present application, the following examples are given for illustration. It should be pointed out that the following examples do not constitute a limitation on the scope of protection required by the present application.

[0090] The raw materials, reagents or devices used in the following examples are commercially available or can be obtained by known methods unless otherwise specified.

[0091] In the components of the cream of the present application examples 1-7 and comparative examples 1-9, the amount and ratio of the related mixtures are as follows:

[0092] The mixture of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-60, sorbitan isostearate, wherein the weight ratio of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-60, sorbitan isostearate is 45:1:1;

[0093] The mixture of xylitol glucoside, anhydrous xylitol, xylitol, water, wherein the weight ratio of xylitol glucoside, anhydrous xylitol, xylitol and water is 8:6:3:3;

[0094] The mixture of lecithin, water, wherein the weight ratio of lecithin, water is 99.4:0.6;

[0095] The mixture of cetyl stearyl olivate, sorbitan olivate, wherein the weight ratio of cetyl stearyl olivate, sorbitan olivate is 3:2;

[0096] The mixture of phytosteryl oleate, phytosterols, wherein the weight ratio of phytosteryl oleate, phytosterols is 97:3;

[0097] The mixture of soluble collagen, sodium chloride, water, wherein the weight ratio of soluble collagen, sodium chloride and water is 8:1:1;

[0098] The mixture of water, butylene glycol, purslane extract, wherein the mass ratio of water, butylene glycol and purslane extract is 69.4:30:0.6.

[0099] The mixture of phytosteryl oleate, phytosterols in the examples and comparative examples of the present application is purchased from Shaanxi Healites Bioengineering Co., Ltd., Healites PES.

[0100] The components and amounts of the anti-wrinkle cream in Examples 1-7 of the present application are shown in Table 1.

[0101] Table 1: Components and amounts (weight percentage) of the anti-wrinkle cream in Examples 1-7 of the present application

[0102]

[0103]

[0104] Example 1

[0105] Example 1 provides an anti-wrinkle cream, the raw material components and amounts of which are shown in Table 1.

[0106] The preparation method of the anti-wrinkle cream in Example 1 comprises the following steps:

[0107] (1) Put water, a mixture of hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, polysorbate-60 and sorbitan isostearate, disodium EDTA, glycerin, sodium hyaluronate, inositol, a mixture of xylitol-based glucoside, anhydrous xylitol, xylitol, water, butylene glycol, 1,2-hexanediol, a penetration enhancer of lecithin and water into an emulsifying kettle, start heating and stirring, heat to 82℃, constant temperature stirring for 30 minutes, then homogenize until the material is evenly dispersed;

[0108] (2) Put stearyl alcohol, a mixture of cetearyl olivate and sorbitan olivate, caprylic / capric triglyceride, dimethicone, a mixture of phytosteryl oleate and phytosterols, avocado butter, jojoba seed oil into an oil kettle, heat to 82℃, suck into the emulsifying kettle, vacuum homogenize until the material is evenly dispersed, add tocopheryl acetate, tetrahydro methyl pyrimidine carboxylic acid, p-hydroxyacetophenone, continue homogenizing until the material is evenly dispersed, start cooling after vacuum defoaming;

[0109] (3) Cool to 62℃, add β-glucan, ubiquinone, hydroxypropyl tetrahydropyrane triol, adenosine, ginseng root extract, acetyl hexapeptide-8, a mixture of soluble collagen, sodium chloride and water, stir until the material is evenly dispersed;

[0110] (4) Continue to cool to 42℃, add dipotassium glycyrrhizinate, a mixture of water, butylene glycol and purslane extract, white spring chamomile flower oil, stir until the material is evenly dispersed;

[0111] (5) After sampling and testing, filter the material, and prepare the anti-wrinkle cream.

[0112] Examples 2-7

[0113] The raw material components and amounts of the anti-wrinkle cream provided in Examples 2-7 are shown in Table 1.

[0114] The preparation method of the anti-wrinkle cream provided in Examples 2-7 is the same as that of Example 1.

[0115] Comparative Example 1

[0116] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not add a penetration enhancer of lecithin and water, and the others are the same as Example 1.

[0117] Comparative Example 2

[0118] The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 uses an equal amount of sodium hyaluronate instead of β-glucan in the anti-wrinkle agent composition, and the others are the same as Example 1.

[0119] Comparative Example 3

[0120] The difference between Comparative Example 3 and Example 1 is only that an equal amount of astaxanthin is used to replace ubiquinone in the anti-wrinkle agent composition of Comparative Example 3, and the rest is the same as Example 1.

[0121] Comparative Example 4

[0122] The difference between Comparative Example 4 and Example 1 is only that an equal amount of myo-inositol is used to replace adenosine in the anti-wrinkle agent composition of Comparative Example 4, and the rest is the same as Example 1.

[0123] Comparative Example 5

[0124] The difference between Comparative Example 5 and Example 1 is only that an equal amount of ergothioneine is used to replace hydroxypropyltetrahydro pyrantriol in the anti-wrinkle agent composition of Comparative Example 5, and the rest is the same as Example 1.

[0125] Comparative Example 6

[0126] The difference between Comparative Example 6 and Example 1 is only that an equal amount of black truffle is used to replace ginseng root extract in the anti-wrinkle agent composition of Comparative Example 6, and the rest is the same as Example 1.

[0127] Comparative Example 7

[0128] The difference between Comparative Example 7 and Example 1 is only that an equal amount of arginine / lysine polypeptide is used to replace acetyl hexapeptide-8 in the anti-wrinkle agent composition of Comparative Example 7, and the rest is the same as Example 1.

[0129] Comparative Example 8

[0130] The difference between Comparative Example 8 and Example 1 is only that an equal amount of hydrolyzed collagen is used to replace the mixture consisting of soluble collagen, sodium chloride and water in the anti-wrinkle agent composition of Comparative Example 8, and the rest is the same as Example 1.

[0131] Comparative Example 9

[0132] The difference between Comparative Example 9 and Example 1 is only that the penetration enhancer consisting of lecithin and water is replaced by an equal amount of laurocapram in Comparative Example 9, and the rest is the same as Example 1.

[0133] Performance Test

[0134] The cream prepared in Examples 1-7 and Comparative Examples 1-9 is subjected to DPPH radical scavenging and elastase inhibition tests.

[0135] 1. DPPH radical scavenging test

[0136] (1) Test principle

[0137] According to the free radical injury theory, the excess production of free radicals is the main cause of natural aging and photoaging of the skin, and reducing the production of free radicals and removing aging metabolites has become an effective method to delay skin aging at present. DPPH is a stable long-lived free radical, and its ethanol solution is deep purple with strong absorption near 517 nm. When there is a free radical scavenger, the light absorption of DPPH ethanol solution is weakened due to the pairing of single electron. The degree of fading of DPPH ethanol solution is linearly related to the number of electrons it receives, so the ability of the sample to be tested to scavenge DPPH free radicals, that is, the size of the antioxidant activity, can be evaluated.

[0138] The test method is an in vitro DPPH free radical scavenging test method, which mainly evaluates the antioxidant efficacy by evaluating the scavenging rate of DPPH free radicals of the sample to be tested, and is suitable for evaluating the efficacy of raw materials with DPPH free radical scavenging effect.

[0139] (2) Main equipment and reagents

[0140] a. Analytical balance, ultraviolet visible spectrophotometer, adjustable pipette gun, etc.;

[0141] b. 1,1-diphenyl-2-trinitrobenzene hydrazine, 0.12mg / mL DPPH ethanol solution, etc.

[0142] (3) Test method

[0143] a. Dissolve and dilute the sample to be tested with 95% ethanol to 3.125mg / mL;

[0144] b. Refer to the sample sampling requirements in Table 2, use 10mL test tubes, set up sample tubes (T), sample background (T0), DPPH tubes (C) and solvent background (C0), and each sample tube (T) of each test concentration of each sample needs to set up 3 parallel tubes, and DPPH tube (C) also needs to set up 3 parallel tubes;

[0145] c. Add 1mL of the same concentration of sample solution to each of the sample tubes (T) and the sample background (T0);

[0146] d. Add 3mL of solvent water to all test tubes (T, T0, C, C0) to make up, and mix well;

[0147] e. Add 0.12mg / mL DPPH ethanol solution 1mL to the sample tube (T) and the DPPH tube (C), and replace the sample background (T0) and the solvent background (C0) with 95% ethanol, and gently shake, and stand at room temperature for 5 minutes;

[0148] f. Move the reaction solution in each tube into a 1cm path length cuvette, and measure the absorbance at 517nm by ultraviolet visible spectrophotometer.

[0149] Table 2: Sample loading requirements

[0150] Reagent T-sample tube T0-sample blank C-DPPH tube C0-solvent blank Sample solution (mL) 1 1 - - Water (mL) 2 2 3 3 DPPH ethanol solution (mL) 1 - 1 - 95% ethanol (mL) - 1 - 1 Parallel number 3 times per sample 1 time per sample 3 times per sample 1 time per sample

[0151] g. Result calculation

[0152] DPPH radical scavenging rate (%) = (1 - (T - T0) / (C - C0)) x 100%.

[0153] In the formula: T - sample tube absorbance value, i.e. the absorbance value of the solution after the sample reacts with DPPH;

[0154] T0 - sample background absorbance value;

[0155] C - DPPH tube absorbance value, 3 times the average value, i.e. the absorbance value of the DPPH solution without adding the sample;

[0156] C0 - solvent background absorbance value.

[0157] h. Result determination

[0158] At the same test concentration, the greater the DPPH radical scavenging rate, the stronger the ability of the sample to scavenge DPPH radicals; otherwise, the weaker.

[0159] 2. Elastase inhibition test

[0160] (1) Test principle

[0161] Elastase is mainly synthesized and secreted by fibroblasts, and is a proteolytic enzyme with very high selectivity and specificity. It has a catalytic hydrolysis effect on many amino acids such as glycine, leucine, alanine, valine, etc. containing carboxyl polypeptide bond, which can cause the decomposition of elastin in connective tissue protein and lead to skin relaxation and wrinkle formation.

[0162] The principle of the elastase inhibition experiment is that elastase reacts with the enzyme substrate, and the absorbance changes after adding the active substance. The size of the absorbance change reflects the size of the elastase inhibitor inhibition rate.

[0163] This test method is an in vitro method, which mainly evaluates the inhibition rate of the test sample on elastase activity to characterize the anti-wrinkle firming effect, and is suitable for evaluating cosmetics that achieve anti-wrinkle firming effect by inhibiting elastase.

[0164] (2) Test method

[0165] a. Add appropriate amount of buffer solution, 60 mg / mL sample solution or control solution, substrate into 96-well plate, shake to mix, incubate for 20 minutes, then add elastase solution, shake immediately, incubate for 10 minutes, measure absorbance at 410 nm by microplate reader, analyze and process data, calculate the inhibition rate of the sample on elastase;

[0166] The substrate is 1.0 mmol / L S4760 (protein substrate For-Ala-Ala-Pro-Abu-SBzl) configured by dimethyl sulfoxide (DMSO) solvent;

[0167] The buffer solution is Tris-HCl buffer solution;

[0168] The elastase solution is 3 U / mL elastase aqueous solution;

[0169] The sample solution is configured by Tris-HCl buffer solution.

[0170] b. Result calculation

[0171] Elastase inhibition rate (%) = [1-(A-B) / (C-D)] x 100%.

[0172] In the formula: A is the absorbance of the experimental group (sample group) (containing substrate, buffer solution, elastase solution, sample solution) / absorbance of quercetin solution (positive control group) / absorbance of water (negative control group);

[0173] B is the absorbance of the experimental blank group (containing substrate, containing buffer solution, not containing elastase solution, containing sample solution) / absorbance of quercetin solution (positive control group) / absorbance of water (negative control group);

[0174] C is the absorbance of the control group (containing substrate, containing buffer solution, containing elastase solution, not containing sample solution);

[0175] D is the absorbance of the blank group (containing substrate, containing buffer solution, not containing elastase solution, not containing sample solution).

[0176] The performance test results of the anti-wrinkle cream of Examples 1-7 and Comparative Examples 1-9 are shown in Table 3.

[0177] Table 3: Performance test results of the anti-wrinkle cream of Examples 1-7 and Comparative Examples 1-9

[0178] Group Radical (DPPH) scavenging rate average (%) Elastase inhibition rate (%) Example 1 28.37 28.49 Example 2 27.54 27.35 Example 3 25.87 26.54 Example 4 18.93 24.27 Example 5 21.46 19.76 Example 6 22.67 25.67 Example 7 20.23 20.17 Comparative Example 1 12.73 14.28 Comparative Example 2 15.39 15.03 Comparative Example 3 14.58 10.54 Comparative Example 4 13.07 12.39 Comparative Example 5 10.26 14.21 Comparative Example 6 16.27 15.01 Comparative Example 7 14.53 13.33 Comparative Example 8 15.89 12.96 Comparative Example 9 15.64 14.37

[0179] As can be seen from Table 3, the cream of the present application has good ability to scavenge DPPH free radicals and inhibit elastase activity, i.e. obvious anti-wrinkle effect.

[0180] Comparative Example 1 does not add the penetration enhancer composed of lecithin and water, Comparative Examples 2-8 respectively replace β-glucan, ubiquinone, adenosine, hydroxypropyl tetrahydro pyran triol, ginseng root extract, acetyl hexapeptide-8, the mixture composed of soluble collagen, sodium chloride and water with other substances, and Comparative Example 9 replaces the penetration enhancer composed of lecithin and water with an equal amount of laurocapram. The ability of the cream of Comparative Examples 1-9 to scavenge DPPH free radicals and the ability to inhibit elastase activity are both significantly worse than Example 1, i.e. the anti-wrinkle effect is worse than Example 1. This shows that the specific combination of penetration enhancer and anti-wrinkle agent composition and the specific combination of components have synergistic effects, so that the cream has better ability to scavenge DPPH free radicals and better ability to inhibit elastase activity, and good anti-wrinkle effect. The absence of any one component will significantly reduce the anti-wrinkle effect of the cream.

[0181] In summary, the present application uses the penetration enhancer lecithin and the anti-wrinkle agents β-glucan, ubiquinone, hydroxypropyl tetrahydro pyran triol, adenosine, ginseng root extract, acetyl hexapeptide, soluble collagen in combination, and there is synergistic effect between the components, which can significantly improve the anti-wrinkle effect, and in combination with other components, the cream of the present application has high elastase inhibition rate and high DPPH free radical scavenging rate, and has good anti-wrinkle effect.

[0182] The above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A face cream, characterized in that, Including anti-wrinkle compositions and penetration enhancers; The anti-wrinkle composition includes β-glucan, ubiquinone, hydroxypropyl tetrahydropyranotriol, adenosine, ginseng root extract, acetyl hexapeptide, and substance A; Substance A includes soluble collagen; The penetration enhancer includes lecithin.

2. The face cream according to claim 1, characterized in that, The face cream also includes moisturizers, emollients, skin conditioning agents, antioxidants, thickeners, chelating agents, emulsifiers, and water.

3. The face cream according to claim 2, characterized in that, The moisturizer includes at least one of glycerin, sodium hyaluronate, inositol, butylene glycol, a mixture of xylitol glucoside, dehydrated xylitol, xylitol and water, and 1,2-hexanediol; And / or, the emollient includes at least one of caprylic / capric triglyceride, polydimethicone, shea butter, a mixture of phytosterol oleate and phytosterols, and jojoba seed oil; And / or, the skin conditioning agent comprises at least one of dipotassium glycyrrhizate, a mixture of water, butylene glycol and purslane extract, and white chamomile flower oil; And / or, the antioxidant includes at least one of tocopherol acetate, tetrahydromethylpyrimidine carboxylic acid, and p-hydroxyacetophenone; And / or, the thickener comprises at least one of a mixture of hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, polysorbate, sorbitan isostearate, and stearyl alcohol.

4. The face cream according to claim 2, characterized in that, By weight percentage, the face cream comprises 0.11-16% anti-wrinkle composition, 0.1-5% penetration enhancer, 1.3-34% moisturizer, 1.2-24% emollient, 0.010-6.5% skin conditioning agent, 0.18-6% antioxidant, 0.2-7% thickener, 0.001-0.3% chelating agent, 0.1-5% emulsifier, and the balance being water.

5. The face cream according to claim 4, characterized in that, The anti-wrinkle composition comprises, by weight percentage: 0.001-0.5% β-glucan, 0.001-0.3% ubiquinone, 0.1-10% hydroxypropyl tetrahydropyranotriol, 0.01-2% adenosine, 0.01-2% ginseng root extract, 0.00001-0.05% acetyl hexapeptide, and 0.00001-0.1% substance A; and / or, substance A further comprises sodium chloride and water.

6. The face cream according to claim 5, characterized in that, In substance A, the weight ratio of soluble collagen, sodium chloride, and water is 8:(0.8-1.2):(0.8-1.2). And / or, the penetration enhancer further includes water; wherein the weight ratio of lecithin to water in the penetration enhancer is 99.4:(0.5-0.7).

7. The face cream according to claim 3, characterized in that, The moisturizer comprises glycerin, sodium hyaluronate, inositol, butylene glycol, 1,2-hexanediol, a mixture of xylitol glucoside, dehydrated xylitol, xylitol, and water; and by weight percentage, the moisturizer comprises 1-10% glycerin, 0.001-1% sodium hyaluronate, 0.1-3% inositol, 0.1-10% butylene glycol, 0.1-5% 1,2-hexanediol, and 0.1-2% of a mixture of xylitol glucoside, dehydrated xylitol, xylitol, and water; And / or, the emollient comprises caprylic / capric triglyceride, dimethicone, shea butter, jojoba seed oil, and a mixture of phytosterol oleate and phytosterols; and by weight percentage, the emollient comprises 1-8% caprylic / capric triglyceride, 0.1-5% dimethicone, 0.1-5% shea butter, 0.01-1% jojoba seed oil, and 0.1-5% a mixture of phytosterol oleate and phytosterols; And / or, the skin conditioning agent comprises dipotassium glycyrrhizate, chamomile flower oil, and a mixture of water, butylene glycol, and purslane extract; and by weight percentage, the skin conditioning agent comprises 0.001-0.5% dipotassium glycyrrhizate, 0.001-1% chamomile flower oil, and 0.01-5% of the mixture of water, butylene glycol, and purslane extract.

8. The method for preparing the face cream according to any one of claims 1-7, characterized in that, The preparation method includes the following steps: The components are mixed to obtain the face cream.

9. The preparation method according to claim 8, characterized in that, The preparation method includes the following steps: (1) Mix water, thickener, chelating agent, humectant and penetration enhancer, heat and keep warm to obtain mixture 1; (2) Mix the emulsifier and emollient, heat, and then mix with the mixture 1 and antioxidant to obtain mixture 2; after cooling, mix with the anti-wrinkle composition and skin conditioning agent to obtain the face cream.

10. The application of the face cream according to any one of claims 1-7 in the field of skin care products.

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