A mask containing natural agarwood extract and a preparation method thereof

Abstract

This invention discloses a facial mask containing natural agarwood extract and its preparation method. The mask comprises the following raw materials in parts by weight: 5-10 parts agarwood extract, 1-5 parts wheat germ extract, 2-5 parts tremella extract, 1-4 parts superoxide dismutase, and 0.5-4.5 parts Bacillus fermentation product. This facial mask possesses the ability to synergistically scavenge ABTS free radicals and synergistically promote the production of type III collagen. On the one hand, it reduces oxidative damage at the source, slowing down the aging process; on the other hand, the production of type III collagen repairs and regenerates the skin, making it firmer and more elastic. The facial mask of this invention synergistically enhances the skin barrier from different aspects, achieving a better synergistic beauty effect. Furthermore, the facial mask of this invention also has good brightening, soothing, and calming effects, and is safe, effective, natural, and low-irritant.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and in particular relates to a facial mask containing natural agarwood extract and its preparation method. Background Technology

[0002] Facial masks belong to the skincare category of cosmetics and have one or more functions such as moisturizing, whitening, anti-aging, and regulating oil balance. Their mechanism of action lies in the fact that when a mask is applied to the face, it raises the skin temperature, opens facial pores, accelerates sweat gland secretion and metabolism, increases the skin's oxygen content, and helps the skin expel metabolic waste and oil. At the same time, the moisture and nutrients in the mask penetrate to the skin's surface, making the skin softer, thus making it look more radiant and elastic.

[0003] As we age, our skin inevitably undergoes the aging process. Environmental factors, such as ultraviolet radiation, dry climates, and pollution, further exacerbate skin damage and accelerate the aging process. To address this issue, various functional face masks have emerged on the market. Despite the wide variety of face masks available, their effects are often relatively singular and vary considerably. Most primarily focus on hydration, or even those offering multiple functions, but the effectiveness is inconsistent and their overall performance is weak. This makes it difficult for many products to meet consumer expectations. Summary of the Invention

[0004] To address the shortcomings of the existing technology, this invention provides a facial mask containing natural agarwood extract. This mask possesses the ability to synergistically scavenge ABTS free radicals and synergistically promote the production of type III collagen. On the one hand, it reduces oxidative damage at its source, slowing down the aging process; on the other hand, the production of type III collagen repairs and regenerates the skin, making it firmer and more elastic. This invention's mask synergistically strengthens the skin barrier from different aspects, achieving a better overall beauty effect. Furthermore, this invention's mask also has good brightening, soothing, and calming effects, and is safe, effective, natural, and low-irritant.

[0005] The purpose of this invention is to provide a facial mask containing natural agarwood extract, comprising the following raw materials in parts by weight: 5-10 parts agarwood extract, 1-5 parts wheat germ extract, 2-5 parts tremella extract, 1-4 parts superoxide dismutase, and 0.5-4.5 parts Bacillus fermentation product.

[0006] In some embodiments of the present invention, the extraction process of the agarwood extract includes the following steps:

[0007] The agarwood was crushed, and the powder was extracted with supercritical carbon dioxide. The extract was collected, filtered, and the agarwood extract was obtained.

[0008] In some embodiments of the present invention, the extraction temperature is 25~35℃, the extraction pressure is 6~8MPa, the CO2 flow rate is 10~30L / h, and the extraction time is 1~3 hours.

[0009] In some embodiments of the present invention, the extraction temperature is 31.1°C, the extraction pressure is 7.38 MPa, the CO2 flow rate is 20 L / h, and the extraction time is 2 hours.

[0010] In some embodiments of the present invention, the extraction process of the wheat germ extract includes the following steps:

[0011] Wheat germ was crushed, water was added, and the mixture was heated to extract it. The mixture was then centrifuged, and the supernatant was concentrated to obtain wheat germ extract.

[0012] In some embodiments of the present invention, the mass ratio of wheat germ to water is 1:8~15.

[0013] In some embodiments of the present invention, the mass ratio of wheat germ to water is 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, or 1:15.

[0014] In some embodiments of the present invention, the heating extraction temperature is 50~60℃ and the time is 3~5 hours.

[0015] In some embodiments of the present invention, the extraction process of the Tremella fuciformis extract includes the following steps:

[0016] The white fungus is crushed, added to water, soaked, heated to extract, filtered, and the filtrate is concentrated to obtain the white fungus extract.

[0017] In some embodiments of the present invention, the mass ratio of the tremella to water is 1:10~15.

[0018] In some embodiments of the present invention, the mass ratio of the tremella to water includes, but is not limited to, 1:10, 1:11, 1:12, 1:13, 1:14, and 1:15.

[0019] In some embodiments of the present invention, the soaking temperature is room temperature and the soaking time is 1 to 3 hours.

[0020] In some embodiments of the present invention, the heating extraction temperature is 50~60℃ and the time is 2~4 hours.

[0021] In some embodiments of the present invention, the facial mask containing natural agarwood extract further includes 0.001 to 0.005 parts of arginine / lysine polypeptide.

[0022] In some embodiments of the present invention, the facial mask containing natural agarwood extract further includes 1.5 to 5 parts of serum albumin.

[0023] In some embodiments of the present invention, the facial mask containing natural agarwood extract further includes 2.5 to 6 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen.

[0024] In some embodiments of the present invention, the face mask containing natural agarwood extract further includes 70-80 parts of water.

[0025] Another objective of this invention is to provide a method for preparing a facial mask containing natural agarwood extract, comprising the following steps:

[0026] S1. Mix 5-10 parts of agarwood extract, 1-5 parts of wheat germ extract, 2-5 parts of tremella extract, 1-4 parts of superoxide dismutase, 0.5-4.5 parts of Bacillus fermentation product and 70-80 parts of water, heat to 30-45℃, homogenize to obtain a mixture;

[0027] S2. Add 0.001~0.005 parts of arginine / lysine polypeptide, 1.5~5 parts of serum albumin, and 2.5~6 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir evenly, and adjust the pH to 6.5~7.5 to obtain a face mask containing natural agarwood extract.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The facial mask of the present invention has the ability to synergistically scavenge ABTS free radicals and synergistically promote the production of type III collagen. On the one hand, it reduces oxidative damage from the root and slows down the aging process. On the other hand, the production of type III collagen repairs and regenerates the skin, making the skin firmer and more elastic. The facial mask of the present invention synergistically enhances the skin barrier from different aspects to achieve a better synergistic beauty effect.

[0030] (2) The mask of the present invention also has good brightening, soothing and calming effects, and has the advantages of being safe, effective, natural and low-irritant. Detailed Implementation

[0031] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below in conjunction with the embodiments of this invention. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0032] All raw materials used in this invention are commercially available. The mixture of Euglena gracilis polysaccharide and hydrolyzed collagen is commercially known as BioStine HP, manufactured by Yu-Chih Chemical Co., Ltd. (Taiwan, China); superoxide dismutase (SOD) is also known as superoxide dismutase.

[0033] Example 1

[0034] This embodiment provides an agarwood extract, the extraction process of which specifically includes the following steps:

[0035] 10g of agarwood was pulverized to 80-100 mesh. The agarwood powder was placed in a supercritical carbon dioxide extraction vessel. The extraction equipment was turned on, and the extraction conditions were set as follows: extraction temperature 31.1℃, extraction pressure 7.38MPa, CO2 flow rate 20L / h, and extraction time 2h. After the extraction was completed, the extract was collected to obtain agarwood extract.

[0036] Example 2

[0037] This embodiment provides a wheat germ extract, the extraction process of which specifically includes the following steps:

[0038] 10g of wheat germ was pulverized to 80-100 mesh. 120g of water was added to the wheat germ powder, and the mixture was heated at 55℃ for 4 hours. After centrifugation, the supernatant was collected and concentrated to obtain the wheat germ extract.

[0039] Example 3

[0040] This embodiment provides a Tremella fuciformis extract, the extraction process of which specifically includes the following steps:

[0041] Grind 10g of white fungus into 80-100 mesh, add 130g of water to the white fungus powder, soak at room temperature for 2 hours, heat at 55℃ for 3 hours, filter, concentrate the filtrate to obtain white fungus extract.

[0042] Example 4

[0043] This embodiment provides a facial mask containing natural agarwood extract, the preparation process of which specifically includes the following steps:

[0044] S1. Mix 10 parts of agarwood extract, 1 part of wheat germ extract, 5 parts of tremella extract, 1 part of superoxide dismutase, 4.5 parts of Bacillus fermentation product and 70 parts of water, heat to 30~45℃, homogenize to obtain a mixture;

[0045] S2. Add 0.005 parts of arginine / lysine polypeptide, 5 parts of Tremella fuciformis extract, 1.5 parts of serum albumin, and 6 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask containing natural agarwood extract.

[0046] Example 5

[0047] This embodiment provides a facial mask containing natural agarwood extract, the preparation process of which specifically includes the following steps:

[0048] S1. Mix 5 parts of agarwood extract, 5 parts of wheat germ extract, 2 parts of tremella extract, 4 parts of superoxide dismutase, 0.5 parts of Bacillus fermentation product and 80 parts of water, heat to 30~45℃, homogenize to obtain a mixture;

[0049] S2. Add 0.001 parts of arginine / lysine polypeptide, 5 parts of serum albumin, and 2.5 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask containing natural agarwood extract.

[0050] Example 6

[0051] This embodiment provides a facial mask containing natural agarwood extract, the preparation process of which specifically includes the following steps:

[0052] S1. Mix 8 parts of agarwood extract, 2 parts of wheat germ extract, 4 parts of tremella extract, 2 parts of superoxide dismutase, 2 parts of Bacillus fermentation product and 75 parts of water, heat to 30~45℃, homogenize to obtain a mixture;

[0053] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask containing natural agarwood extract.

[0054] Comparative Example 1

[0055] This comparative example provides a facial mask containing natural agarwood extract, the preparation process of which specifically includes the following steps:

[0056] S1. Mix 18 parts of agarwood extract with 75 parts of water, heat to 30~45℃, and homogenize to obtain a mixture;

[0057] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask containing natural agarwood extract.

[0058] Comparative Example 2

[0059] This comparative example provides a facial mask, the preparation process of which specifically includes the following steps:

[0060] S1. Mix 18 parts of wheat germ extract with 75 parts of water, heat to 30-45℃, and homogenize to obtain a mixture;

[0061] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask.

[0062] Comparative Example 3

[0063] This comparative example provides a facial mask, the preparation process of which specifically includes the following steps:

[0064] S1. Mix 18 parts of tremella extract with 75 parts of water, heat to 30~45℃, homogenize until uniform, and obtain a mixture;

[0065] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask.

[0066] Comparative Example 4

[0067] This comparative example provides a facial mask, the preparation process of which specifically includes the following steps:

[0068] S1. Mix 18 parts of superoxide dismutase and 75 parts of water, heat to 30-45℃, and homogenize to obtain a mixture;

[0069] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask.

[0070] Comparative Example 5

[0071] This comparative example provides a facial mask, the preparation process of which specifically includes the following steps:

[0072] S1. Mix 18 parts of Bacillus fermentation product with 75 parts of water, heat to 30-45℃, homogenize to obtain a mixture;

[0073] S2. Add 0.003 parts of arginine / lysine polypeptide, 3.5 parts of serum albumin, and 4 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir well, and adjust the pH to 6.5~7.5 to obtain a face mask.

[0074] Antioxidant capacity test:

[0075] The antioxidant capacity can be reflected by the determination of the free radical scavenging ability of 2,2-adiazonium-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS). The higher the ABTS free radical scavenging rate, the stronger the antioxidant capacity, and vice versa. Refer to GB / T39100-2020 for the determination of peptide antioxidant capacity using the DPPH and ABTS methods. ABTS can be oxidized by K2S2O8 to generate the blue-green free radical cation ABTS. . +, ABTS . The sample exhibits a blue-green color with a maximum absorption peak at 734 nm. As free radicals are scavenged, their numbers decrease, resulting in a lighter solution color and consequently a decrease in absorbance at 734 nm. This allows for the calculation of ABTS scavenging in the sample. . + ability.

[0076] The specific steps are as follows:

[0077] Weigh a certain amount of ABTS . +, ABTS was prepared using anhydrous ethanol. . If the concentration is prepared to have an absorbance of 0.7 ± 0.02 at 734 nm, then this is ABTS. . +Working solution. The experiment was divided into a solvent background group (T...). a Solvent reaction pore group (T) b ), sample background group (T) c ), sample reaction group (T) d Solvent background group (T) a The solvent is anhydrous ethanol; the solvent reaction pore group (T) b ) is ABTS . +Working solution, solvent; Sample background group (T) c The sample consists of anhydrous ethanol; the sample reaction group (T) d ) is ABTS . +Working solution, samples; wherein the samples are the mask solutions (1%) of Examples 4-6 and Comparative Examples 1-5, respectively. Three replicates per group. ABTS . The working solution, the prepared mask solutions (1%) of Examples 4-6 and Comparative Examples 1-5, anhydrous ethanol, and solvent were added sequentially to a 96-well plate according to the amounts shown in Table 1. The mixture was thoroughly mixed and incubated at 30°C for 10 min. The absorbance was measured at 734 nm using a microplate reader. Vitamin C was used as a positive control. The samples were tested against ABTS. . + The scavenging rate of free radicals is calculated using the following formula:

[0078]

[0079] In formula (1):

[0080] A a— Average absorbance of the solvent background pores;

[0081] A b —Average absorbance of solvent reaction wells;

[0082] A c —Average absorbance of the sample's bottom wells;

[0083] A d —Sample bottom well absorbance.

[0084] Table 1. ABTS free radical scavenging ability sampling table.

[0085]

[0086] Table 2. Test results of ABTS free radical scavenging rate.

[0087]

[0088] As shown in Table 2, the antioxidant effect of the mask prepared by the combination of agarwood extract, wheat germ extract, tremella extract, superoxide dismutase, and Bacillus fermentation product is better than that of the mask prepared by using any one of the above components alone, indicating that there is a synergistic antioxidant effect among the above five substances.

[0089] Experiments to promote the synthesis of type III collagen:

[0090] Test samples: The masks prepared in Examples 4-6 and Comparative Examples 1-5 were prepared into sample solutions of 0.01 mg / mL.

[0091] Experimental Methods: Human fibroblasts (Merck Biotech) cultured in DMEM (Dulbecco's Modified Eagle Medium) containing 10% FBS (fetal bovine serum) were seeded into 96-well plates. After cell attachment, the medium was exchanged with DMEM containing 250 μmol / L magnesium ascorbate phosphate and 0.2 wt% FBS. 100 μL of sample solution was added to each well (100 μL of deionized water was added to the control group). After mixing and culturing for 72 h, the supernatant was collected, centrifuged, and the content of type III collagen in the supernatant was determined using a kit. The synthesis promotion rate of type III collagen was calculated. The results are shown in Table 3.

[0092] Type III collagen synthesis promotion rate (%) = (A1-A0) / A0 × 100%;

[0093] A1: Type III collagen production after adding sample solution;

[0094] A0: Type III collagen production without the addition of sample solution.

[0095] Table 3. Test results on the promotion of type III collagen synthesis.

[0096]

[0097] As shown in Table 3, the combination of agarwood extract, wheat germ extract, tremella extract, superoxide dismutase, and Bacillus fermentation product is more effective in promoting the production of type III collagen than the combination of any one of the above components alone. This indicates that there is a significant synergistic effect among the five substances on promoting the production of type III collagen.

[0098] Anti-aging performance test:

[0099] Forty healthy volunteers aged 30-50 were selected and randomly divided into eight groups of five each.

[0100] After cleansing, the mask liquids from Examples 4-6 and Comparative Examples 1-5 were applied to the faces and corners of the eyes of the volunteers, respectively. The masks were allowed to absorb for 20 minutes. The masks were used three times a week for four consecutive weeks. The average values ​​of the corresponding indicators were recorded after four weeks of testing. The results are shown in Table 4.

[0101] Table 4. Test results of the anti-aging properties of the facial mask liquids in Examples 4-6 and Comparative Examples 1-5.

[0102]

[0103] As shown in Table 4, by comparing Examples 4-6 and Comparative Examples 1-5, it can be found that the face mask prepared by combining a specific ratio of agarwood extract, wheat germ extract, tremella extract, superoxide dismutase, and Bacillus fermentation product has a synergistic effect on wrinkle reduction.

[0104] Human skin patch test:

[0105] The irritation and safety of the face masks prepared in Examples 4-6 and Comparative Examples 1-5 were tested.

[0106] Experimental Methods: Refer to Section 2.1, Chapter 7, Occlusive Patch Test, of the 2015 edition of the *Cosmetic Safety Technical Specifications*. 0.02 mL of different facial mask products was weighed into the patch applicator, with a blank control group included. Four hundred healthy volunteers were selected and divided into 8 groups of 50 each, aged 18-24 years, with no history of allergies. After cleaning the inner forearm of the subjects, 0.02 mL of sample was added to the filter paper attached to the patch applicator. The patch applicator was applied to the inner forearm of the subjects using non-irritating adhesive tape, and then gently pressed with fingers to ensure even application to the skin for 24 hours. Subjects were instructed to keep the patch area dry for 24 hours and avoid strenuous exercise, scratching, and prolonged sun exposure. After 24 hours, the applicator was removed and marked. Skin reactions were observed at 30 min, 24 h, and 48 h after removal of the patch applicator according to the standards in Table 5, and the results were recorded.

[0107] Table 5. Grading Criteria for Skin Reactions in Occlusive Patch Tests

[0108]

[0109] Table 6. Results of Skin Patch Test

[0110]

[0111] As shown in Table 6, none of the masks prepared in Examples 4 to 6 had serious adverse reactions, and the subjects had mostly negative reactions. The results indicate that the masks prepared in Examples 4 to 6 had low irritation and high safety.

[0112] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that after reading this application specification, they can still modify or make equivalent substitutions to the specific implementation of the present invention, but these modifications or changes do not depart from the protection scope of the pending claims of the present invention.

Claims

1. A facial mask containing natural agarwood extract, characterized in that, It is made from the following raw materials in parts by weight: 5-10 parts agarwood extract, 1-5 parts wheat germ extract, 2-5 parts tremella extract, 1-4 parts superoxide dismutase, 0.5-4.5 parts Bacillus fermentation product, 0.001-0.005 parts arginine / lysine polypeptide, 1.5-5 parts serum albumin, 2.5-6 parts a mixture of Euglena gracilis polysaccharide and hydrolyzed collagen, and 70-80 parts water; wherein, The extraction process of the agarwood extract includes the following steps: crushing agarwood, extracting the agarwood powder with supercritical carbon dioxide, collecting the extract, filtering, and obtaining the agarwood extract; and the extraction temperature is 25~35℃, the extraction pressure is 6~8MPa, the CO2 flow rate is 10~30L / h, and the extraction time is 1~3 hours. The extraction process of the wheat germ extract includes the following steps: crushing wheat germ, adding water, heating and extracting, centrifuging, concentrating the supernatant to obtain wheat germ extract; and the mass ratio of wheat germ to water in the extraction process of the wheat germ extract is 1:8~15. The extraction process of the tremella extract includes the following steps: pulverizing tremella, adding water, soaking, heating and extracting, filtering, concentrating the filtrate to obtain tremella extract; and the mass ratio of tremella to water in the extraction process of the tremella extract is 1:10~15.

2. The method for preparing the facial mask containing natural agarwood extract as described in claim 1, characterized in that, Includes the following steps: S1. Mix 5-10 parts of agarwood extract, 1-5 parts of wheat germ extract, 2-5 parts of tremella extract, 1-4 parts of superoxide dismutase, 0.5-4.5 parts of Bacillus fermentation product and 70-80 parts of water, heat to 30-45℃, homogenize to obtain a mixture; S2. Add 0.001~0.005 parts of arginine / lysine polypeptide, 1.5~5 parts of serum albumin, and 2.5~6 parts of a mixture of Euglena spp. polysaccharide and hydrolyzed collagen to the mixture, stir evenly, and adjust the pH to 6.5~7.5 to obtain the facial mask containing natural agarwood extract.

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