Streptomyces crimped BJRC1-5 and application thereof

Abstract

The invention provides streptomyces criosus BJRC1-5 and application thereof, and belongs to the technical field of biological prevention and control. The streptomyces crimped strain BJRC1-5 is classified and named as Streptomyces, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 9, 2025, and the preservation number is CGMCC NO.35152. The streptomyces crimped belongs to the bacteria field, actinomycetes, actinomycetes, streptomycetes, streptomycetes, streptomycetes and streptomycetes. The strain disclosed by the invention has a good effect of preventing and treating anise anthracnose and has no influence on the growth of anise.

Description

Technical Field

[0001] This invention relates to the field of biological control technology, specifically to a Streptomyces curvaturei BJRC1-5 and its applications. Background Technology

[0002] star anise( Illicium verum Illicium lanceolatum (also known as star anise) is a plant belonging to the genus Illicium in the family Illicaceae. It is native to subtropical regions of China, primarily concentrated in Guangxi (accounting for over 80% of global production), Yunnan, and Guangdong provinces. It thrives in warm, humid climates (average annual temperature 18-23℃, annual rainfall 1200-2000 mm). As an important economic crop in my country, it is not only an indispensable spice in traditional cooking but also a crucial raw material in the pharmaceutical and chemical industries. Its fruit is rich in anethole oil and shikimic acid, widely used in food seasoning, traditional Chinese medicine (such as the extraction of raw materials for the antiviral drug Tamiflu), and the production of daily chemical products, possessing extremely high economic value. However, star anise cultivation is frequently threatened by anthracnose, a disease caused by *Amycosis anthracnose* (a fungus). Colletotrichum Caused by fungi (spp.), the disease typically presents with brown spots on leaves and black rot and fruit drop. It can lead to a 20%-30% reduction in yield in mild cases and a complete crop failure in severe cases, posing a huge challenge to farmers' income and the stability of the industrial chain.

[0003] Anthracnose is a core disease threatening the star anise industry. While traditional chemical control can suppress the pathogen in the short term, its long-term harm cannot be ignored: First, over-reliance on chemical pesticides leads to significant drug resistance in anthracnose fungi; second, pesticide residues easily accumulate in star anise fruits, threatening the safety of food and medicines made from star anise; third, broad-spectrum fungicides indiscriminately kill soil microorganisms and pollinating insects, disrupting the understory ecological balance and further weakening the system's disease resistance. Currently, promoting a green control system of "microbial agents + disease-resistant varieties + ecological regulation" has become the only way to break through the predicament of chemical control and ensure the high-quality development of the star anise industry. Summary of the Invention

[0004] The purpose of this invention is to propose a Streptomyces curvaturei BJRC1-5 and its application, which has a good effect on the prevention and control of anthracnose in star anise and has no effect on the growth of star anise.

[0005] The technical solution of this invention is implemented as follows: The application provides a Streptomyces cirratus strain BJRC1-5, the classification name of the Streptomyces cirratus strain BJRC1-5 is Streptomyces cirratus, the preservation unit is China General Microbiological Culture Collection Center, the address is No. 1, Beichen West Road, Haidian District, Beijing, China, the Institute of Microbiology of Chinese Academy of Sciences, the post code is 100080, the preservation date is July 9, 2025, the preservation number is CGMCC NO. 35152, and the Streptomyces cirratus belongs to the bacterial kingdom, the actinobacteria, the actinobacteria class, the Streptomyces order, the Streptomyces family and the Streptomyces genus.

[0006] As a further improvement of the application, the 16S rRNA sequence of the Streptomyces cirratus strain BJRC1-5 is shown in SEQ ID No. 1.

[0007] As a further improvement of the application, the strain has good disease inhibition effect on Illicium verum anthracnose, can inhibit the growth of pathogenic bacteria and lyse pathogenic bacterial spores, and does not affect the normal growth of Illicium verum.

[0008] The pathogenic bacteria of Illicium verum anthracnose can be inhibited on the 4th day of confrontation culture, and the inhibition phenomenon is more obvious on the 7th day of confrontation culture, the inhibition rates of the BJRC1-5 on three strains of pathogenic bacteria are 32.55 ± 3.15%, 21.34 ± 3.57% and 21.53 ± 1.27% respectively, p < 0.05 reaches significant inhibition, and the inhibition zones are 5, 4 and 6 mm respectively.

[0009] After the strain is treated with 70% (v / v) concentration of fermentation liquor by the moist co-culture method for 6 hours, the spore inhibition rate reaches 60.74 ± 0.54%, and it is observed by an optical microscope that the conidium wall of the pathogenic bacteria is broken and the contents flow out, and the sterile fermentation liquor of the strain significantly inhibits the normal germination and appressorium formation of the conidium.

[0010] The strain is obtained through the following specific steps: A, sample collection: healthy Illicium verum root samples collected from Funing County, Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province.

[0011] B, isolation and culture of the strain: the endophytic actinomycetes in the healthy Illicium verum root samples are separated by the dilution coating plate method: the endophytic actinomycetes in the healthy Illicium verum root samples are separated by the dilution coating plate method: 1 mL of sterile water, 2 g of root samples, a steel bead homogenizer is added, centrifuged to take supernatant, respectively diluted by 10 -1 , 10 -2 , 10 -3 , 10 -4Coated on TWYE medium plate with 50 mL diluent per plate, incubated at 28℃ for 7-10 d. After colony formation, typical single colonies were picked with a sterile inoculation loop, and the strain was purified on PDA medium by streaking method.

[0012] C. Strain preservation: the pure colonies of the biocontrol preparation strain obtained in the experiment were inoculated on test tube slant, and incubated at 28℃ for 10 d. After the colonies were full, the preservation was carried out in a refrigerator at 4℃. The preservation medium was PDA medium.

[0013] TWYE medium: yeast extract 0.25 g, potassium dihydrogen phosphate 0.5 g, agar 20 g, and distilled water was added to 1 L, pH=7.0.

[0014] Potato glucose agar medium (PDA) ingredients are as follows in g / L: potato 200 g, glucose 20 g, agar 20 g, distilled water 1 L, and pH is natural.

[0015] The specimen collection site in step A is Funing County, Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province.

[0016] The specimen in step A is collected from the healthy root system soil of star anise in Funing County, Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province.

[0017] In the sterile operation table in step B, the operation is carried out under an alcohol lamp, and the inoculation needle, inoculation ring, and all separation steps are operated under alcohol.

[0018] The sterilization in step B is to place the inoculation needle, inoculation ring, sterile water, tweezers, and empty medium required for the test in a high-pressure steam sterilizer, and sterilize at 121℃ for 30 min.

[0019] The preferred separation culture condition in step B is to incubate at 28℃ in the dark for 7-10 d.

[0020] The preferred purification culture and preservation condition in step C is to incubate at 28℃ in the dark for 10 d, and then store at 4℃.

[0021] The application further protects the application of the above-mentioned Streptomyces anulatus strain BJRC1-5 in the biological control of star anise anthracnose.

[0022] As a further improvement of the application, the Streptomyces anulatus strain BJRC1-5 has an inhibitory effect on the pathogenic bacteria of star anise anthracnose.

[0023] As a further improvement of the application, the pathogenic bacteria of star anise anthracnose include BJYB3-20 Colletotrichum gloeosporioides ( Colletotrichum fructicola ), BJYB3-22 Colletotrichum harzianum ( Colletotrichum horii), BJYB3-22 Guignardia harae (Sawada) Yamamoto ( Colletotrichum gloeosporioides ) and BJYB3-29 Guignardia bidwellii (Berk. et Curt.) Vink (

[0024] A method for biocontrol of Illicium lanceolatum anthracnose by the curled Streptomyces strain (BJRC1-5) includes screening of biocontrol strains and determination of biocontrol effect, and specifically includes the following steps: Streptomyces cirratus A, screening of biocontrol strains: cross method is used for preliminary screening of antagonistic bacteria. The specific operation is as follows: the pathogenic fungi BJYB3-20 Guignardia fructigenum (Lev.) Vink ( Colletotrichum fructicola ), BJYB3-22 Guignardia harae (Sawada) Yamamoto ( Colletotrichum horii Colletotrichum gloeosporioides ), and BJYB3-29 Guignardia bidwellii (Berk. et Curt.) Vink (

[0025] ) are used as indicator bacteria, 6 mm agar blocks of the pathogenic fungi are inoculated at the center of PDA medium plate, then the single bacterial colonies obtained by isolation and culture are inoculated at a distance of 2.5 cm from the pathogenic fungi, 3 repeats are set, and after 4-7 days of constant temperature culture at 28℃, the size of the inhibition zone is observed, and the strain with the best antagonistic effect is selected. Colletotrichum fructicola B, determination of biocontrol effect: in order to screen strains with strong inhibition activity, the plate confrontation growth method is used for rescreening of the strains obtained by preliminary screening: the pathogenic fungi BJYB3-20 Guignardia fructigenum (Lev.) Vink ( Colletotrichum horii Colletotrichum gloeosporioides Colletotrichum fructicola ), BJYB3-22 Guignardia harae (Sawada) Yamamoto ( Colletotrichum horii Colletotrichum gloeosporioides ) and BJYB3-29 Guignardia bidwellii (Berk. et Curt.) Vink ( ) are used as indicator bacteria, first, the activated pathogenic fungus agar blocks are punched by a 6 mm puncher and placed on the plate, then the antagonistic bacteria are inoculated by point inoculation method, the distance between the two bacteria is 2.5 cm, the control group is only inoculated with pathogenic fungus agar blocks, 3 repeats are set, and after 4-7 days of culture in a 28℃ incubator, the growth of the pathogenic fungi is observed, the inhibition zone is measured, the inhibition rate is calculated, and the best antagonistic bacteria are finally screened. The inhibition rate calculation formula is: inhibition rate (%) = (control pathogenic fungus colony diameter - treated pathogenic fungus colony diameter) / control pathogenic fungus colony diameter x 100%C. To evaluate the effect of fermentation broth on conidia, the strain BJRC1-5 was subcultured to D600nm=0.5, and then expanded culture for 10 days at an inoculation amount of 1%(v / v). The supernatant was collected by centrifugation at 10000 r / min for 10 min, and then sterilized by 0.22 mm filter membrane for standby. The initial fermentation broth was configured into 10%, 30%, 50%, 70% and 90% (v / v) fermentation broth respectively, and then resuspended conidia were cultured at 28℃ in dark and moisture for 2, 4, 6 and 8 h. The sterile LB liquid medium was used as negative control, and each treatment was set with 3 repeats. Finally, the fermentation broth with the best inhibition effect on spore germination was screened. The spore germination inhibition rate calculation formula was: spore germination inhibition rate=(control group spore germination rate-treatment group spore germination rate) / control group spore germination rate*100% TWYE medium: yeast extract 0.25 g, potassium dihydrogen phosphate 0.5 g, agar 20 g, constant volume to 1 L with distilled water, pH=7.0.

[0026] Potato glucose agar medium (PDA) ingredients are as follows: potato 200 g, glucose 20 g, agar 20 g, distilled water 1 L, pH natural.

[0027] The present application has the following beneficial effects: 1. The strain has good effect on preventing and treating anthracnose of star anise, and has no influence on the growth of star anise.

[0028] 2. The strain can inhibit the pathogen of anthracnose of star anise on the 4th day of confrontation culture, and the inhibition phenomenon is more obvious on the 7th day of confrontation culture.

[0029] 3. The strain adopts the moist co-culture method, and the conidia wall is observed to be broken and the contents are discharged after the fermentation broth with a concentration of 70% (v / v) is treated for 6 h, and the sterile fermentation broth of the strain can significantly inhibit the normal germination of conidia and the formation of appressorium.

[0030] 4. The strain is easy to obtain, and the culture time is short, which is beneficial to large-scale production and popularization and application. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings in the following description only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0032] Figure 1Streptomyces anulatus (BJRC1-5) in PDA medium after 7 days of incubation at 28°C. Streptomyces cirratus Figure 1. Pure culture morphology of Streptomyces anulatus (BJRC1-5) in PDA medium after 7 days of incubation at 28°C. A: Medium front; B: Medium back.

[0033] Figure 2 Streptomyces anulatus (BJRC1-5) based on the confrontation method on plate Streptomyces cirratus Antagonistic effect of Streptomyces anulatus (BJRC1-5) on Guignardia bidwellii strain (BJYB3-20); A: BJYB3-20 (pure culture front and back); B: BJYB3-20 control group; C: BJRC1-5 Colletotrichum BJYB3-20 confrontation culture group. fructicola vs. Streptomyces anulatus (BJRC1-5) based on the confrontation method on plate

[0034] Figure 3 Antagonistic effect of Streptomyces anulatus (BJRC1-5) on Guignardia bidwellii strain (BJYB3-22); A: BJYB3-22 (pure culture front and back); B: BJYB3-22 control group; C: BJRC1-5 Streptomyces BJYB3-22 confrontation culture group. cirratus Colletotrichum horii Antagonistic effect of Streptomyces anulatus (BJRC1-5) on Guignardia bidwellii strain (BJYB3-29); A: BJYB3-29 (pure culture front and back); B: BJYB3-29 control group; C: BJRC1-5 vs. BJYB3-29 confrontation culture group.

[0035] Figure 4 Antagonistic effect of Streptomyces anulatus (BJRC1-5) on Guignardia bidwellii strain (BJYB3-29); A: BJYB3-29 (pure culture front and back); B: BJYB3-29 control group; C: BJRC1-5 Streptomyces BJYB3-29 confrontation culture group. cirratus Colletotrichum gloeosporioides Spore lysis effect of Streptomyces anulatus (BJRC1-5) fermentation broth on Guignardia bidwellii strain (BJYB3-29); A: Untreated spores 6 h (control group); B: Fermentation broth treated 6 h (treatment group). vs. DETAILED DESCRIPTION

[0036] Figure 5 Spore lysis effect of Streptomyces anulatus (BJRC1-5) fermentation broth on Guignardia bidwellii strain (BJYB3-29); A: Untreated spores 6 h (control group); B: Fermentation broth treated 6 h (treatment group). Streptomyces cirratus Colletotrichum Spore lysis effect of Streptomyces anulatus (BJRC1-5) fermentation broth on Guignardia bidwellii strain (BJYB3-29); A: Untreated spores 6 h (control group); B: Fermentation broth treated 6 h (treatment group). gloeosporioides DETAILED DESCRIPTION

[0037] ​The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] This invention relates to a Streptomyces cirratus strain BJRC1-5, which is classified and named Streptomyces cirratus. The strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100080, China. The deposit date is July 9, 2025, and the accession number is CGMCCNO.35152. The Streptomyces cirratus belongs to the kingdom Bacteria, phylum Actinobacteria, class Actinobacteria, order Streptomycetes, family Streptomycetes, and genus Streptomyces.

[0039] Example 1: Streptomyces curvatureus ( Streptomyces cirratus Acquisition, identification and preservation of BJRC1-5 (1) Streptomyces curvatureus ( Streptomyces cirratus Acquisition and identification of BJRC1-5.

[0040] The *Streptomyces curvaturei* strain described in this invention ( Streptomyces cirratus BJRC1-5 was isolated from the root soil of healthy star anise in Funing County, Yunnan Province. The samples were collected from Funing County, Wenshan Zhuang and Miao Autonomous Prefecture, Yunnan Province, and stored at 4℃ for later use. Endophytic actinomycetes were isolated from the root samples of healthy star anise using the dilution-spreading method: 1 ml of sterile water and 2 g of root sample were added to a steel ball mill for homogenization, centrifugation was performed, and the supernatant was diluted 10⁻⁶ times. -1 10 -2 10 -3 10 -4 Add 50 μL of diluent to each plate and spread it on TWYE agar plates. Incubate at 28°C for 7–10 days. After colony formation, pick out typical single colonies with a sterile inoculation loop and purify the strain on PDA medium using the streak method.

[0041] TWYE medium: 0.25 g yeast extract, 0.5 g potassium dihydrogen phosphate, 20 g agar, diluted to 1 L with distilled water, pH=7.0.

[0042] Potato glucose agar (PDA) medium consists of the following components in g / L: 200 g potato, 20 g glucose, 20 g agar, 1 L distilled water, and natural pH.

[0043] The morphological and molecular biological identification results of the isolated strain BJRC1-5 are recorded below: A. Morphological characteristics: The colonies of strain BJRC1-5 are orange-yellow, nearly round to irregular in shape, with smooth protrusions on the surface. The middle of the colony is fluffy, and it becomes powdery in the later stage, and becomes dry and opaque.

[0044] B. 16S rDNA sequence analysis: The 16S rDNA sequence of the strain was determined, yielding *Streptomyces curvaturei* (…). Streptomyces cirratus The 16S rDNA sequence of BJRC1-5 is shown in SEQ ID NO.1. Using total DNA from strain CPAT-W06 as a template, the 16S rDNA fragment of this strain was amplified by PCR using the universal 16S rDNA primers for actinomycetes 8-27F (5'-AGAGTTTGATCCTGGCTCAG-3') / 1523-1504R (5'-TTAAGGTGATCCAGCCGCA-3'). The PCR reaction system was 2×Power... Taq PCR MasterMix: 12.5 µL, Template DNA: 1 µL, Forward primer (10 μmol / L): 1 µL, Reverse primer (10 μmol / L): 1 µL, ddH2O: 9.5 µL, Total volume: 25 µL. PCR reaction program: After pre-denaturation at 95℃ for 5 min, the following cycles were performed: denaturation at 95℃ for 1 min, annealing at 55℃ for 1 min, extension at 72℃ for 2 min, for 35 cycles. After the final extension at 72℃ for 10 min, the PCR instrument was stored at 4℃.

[0045] The PCR product was sent to Kunming Shuoqing Biotechnology Co., Ltd. for purification and sequencing. After sequence assembly, it was submitted to EzBioCloud (https: / / www.ezbiocloud.net / ) for comparison. The comparison results showed that strain BJRC1-5 was similar to Streptomyces curvaturei strain ( Streptomyces cirratus ) The 16S rDNA sequence similarity of (NRRL B-3250) reached 99.93%. Based on sequence alignment and morphological characteristics, strain BJRC1-5 was identified as a Streptomyces curvaturei strain. Streptomyces cirratus The sequence of strain BJRC1-5 described in this invention is shown in the sequence listing.

[0046] (2) Streptomyces curvaturei strain ( Streptomyces cirratus Preservation of BJRC1-5.

[0047] Based on the above identification results, the strain was confirmed to be *Streptomyces curvaturei* strain ( Streptomyces cirratus), and was preserved in China General Microbiological Culture Collection Center (CGMCC) on July 24, 2025, and the preservation number is CGMCC NO. 35152.

[0048] Example 2 Streptomyces Strain (BJRC1-5) Streptomyces cirratus Screening of BJRC1-5 strain (1) Screening of the strain.

[0049] The cross method was used for the preliminary screening of the antagonistic bacteria. The specific operation is as follows: the pathogens of the eight-angle anthracnose disease, i.e., Colletotrichum gloeosporioides (BJYB3-20) Colletotrichum fructicola ), C. acutatum (BJYB3-22) Colletotrichum horii ), and C. gloeosporioides (BJYB3-29) Colletotrichum gloeosporioides were used as indicator bacteria, 6 mm agar blocks of the pathogenic bacteria were inoculated at the center of PDA medium plates, and then the single bacterial colonies obtained by isolation and culture were inoculated at a distance of 2.5 cm from the pathogenic bacteria, three replicates were set, and after constant temperature culture at 28°C for 4-7 days, the size of the inhibition zone was observed, and the strain with the best antagonistic effect was selected.

[0050] (2) Observation of results.

[0051] The cross method was used for screening the strains isolated, and the results showed that there were 8 strains that had antagonistic effect on the eight-angle anthracnose disease.

[0052] Example 3 Streptomyces (BJRC1-5) Strain Streptomyces cirratus Determination of the biocontrol effect of BJRC1-5 strain.

[0053] (1) Determination of the biocontrol effect.

[0054] Confrontation culture method: the pathogens of the eight-angle anthracnose disease, i.e., C. gloeosporioides (BJYB3-20) Colletotrichum fructicola ), C. acutatum (BJYB3-22) Colletotrichum horii ), and C. gloeosporioides (BJYB3-29) Colletotrichum gloeosporioides) as an indicator bacteria, first use 6 mm puncher to take activated pathogenic bacteria agar block, placed on the plate, then use point connection method to inoculate antagonistic bacteria, two bacteria 2.5 cm apart, the control group only inoculates pathogenic bacteria agar block, sets 3 repeats, 28 DEG C incubator culture 4~7 d, observes pathogenic bacteria growth, measures antibacterial band, calculates antibacterial rate, finally selects the best antagonistic effect of antagonistic bacteria. Antibacterial rate calculation formula: antibacterial rate (%)=(control pathogenic bacteria colony diameter-treatment pathogenic bacteria colony diameter) / control pathogenic bacteria colony diameter*100% To evaluate the effect of fermentation broth on conidium, the strain BJRC1-5 was subcultured to D600nm=0.5, and then expanded culture for 10 days at a 1%(v / v) inoculation amount.After centrifugation at 10000 r / min for 10 min, the supernatant was collected and sterilized with a 0.22 μm filter membrane for standby use.The initial fermentation broth was configured into 10%, 30%, 50%, 70% and 90% (v / v) fermentation broth respectively, and the conidium was resuspended and cultured at 28℃ in the dark for 2, 4, 6 and 8 h.The sterile LB liquid medium was used as a negative control, and each treatment was set in triplicate.Finally, the best fermentation broth concentration for inhibiting spore germination was screened.The spore germination inhibition rate calculation formula was: spore germination inhibition rate=(control group spore germination rate-treatment group spore germination rate) / control group spore germination rate*100% (2) Results observation.

[0055] As Figures 2-4 , the strain can observe that the Ganoderma boninense pathogen is inhibited on the 4th day of face-to-face culture, and the inhibition phenomenon is more obvious on the 7th day of face-to-face culture, and the inhibition rates of the three pathogenic bacteria are 32.55±3.15%, 21.34±3.57% and 21.53±1.27% respectively, and the antibacterial bands are 5, 4 and 6 mm respectively.

[0056] As Figure 5 , after the strain is treated with 70% (v / v) concentration fermentation broth for 6 h by using the moist co-culture method, the spore inhibition rate reaches 60.74±0.54%, and it is observed by optical microscope that the conidium wall of the pathogenic bacteria is broken and the contents flow out, and the sterile fermentation broth of the strain significantly inhibits the normal germination and appressorium formation of conidium.

[0057] The above only describes the preferred embodiments of the present application and should not be used to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A Streptomyces curvatureii strain BJRC1-5, characterized in that, The described *Streptomyces cirratus* strain BJRC1-5 is classified as *Streptomyces cirratus*, deposited at the China General Microbiological Culture Collection Center (CGMCC) on July 9, 2025, with accession number CGMCCNO.35152. *Streptomyces cirratus* belongs to the kingdom Bacteria, phylum Actinobacteria, class Actinobacteria, order Streptomycetes, family Streptomycetes, and genus *Streptomyces*.

2. The *Streptomyces curvatureii* strain BJRC1-5 according to claim 1, characterized in that, The 16S rRNA sequence of the Streptomyces curvatureis strain BJRC1-5 is shown in SEQ ID No.

1.

3. The application of the *Streptomyces curvaturei* strain BJRC1-5 as described in claim 1 or 2 in the biological control of anthracnose.

4. The application according to claim 3, characterized in that, The described Streptomyces curvature strain BJRC1-5 has an inhibitory effect on the anthrax pathogen.

5. The application according to claim 4, characterized in that, The anthracnose pathogens mentioned include BJYB3-20 fruit anthracnose fungus (… Colletotrichum fructicola ), BJYB3-22 Hari anthrax bacteria ( Colletotrichum horii ), BJYB3-29 Colloidal anthrax bacteria ( Colletotrichum gloeosporioides ).

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