Seed sample cutting female parent and sample corresponding type collecting and coding system

Abstract

The invention relates to the technical field of breeding, in particular to a seed sample cutting female parent and sample corresponding type collecting and coding system which comprises a motion control system, a counter and a cutting completion signal module. The motion control system is electrically connected with the first motion module and the second motion module; a first grid position for controlling the first movement module to support the sample box and a first grid position for controlling the second movement module to support the collection box are located under the initial collection position; the numerical value of the counter is set as N, and the initial value is 1; and after a driving instruction is generated according to the cutting completion signal every time, the value N of the counter is increased by 1. By arranging a synchronous motion control system and a counter, synchronous linkage driving and accurate site coding of a sample box and a female parent collecting box are completed, and the technical effect that after one-time successful cutting, a sample and a female parent automatically fall into physical grids with the same serial number is achieved; and the technical effect of establishing the traceable electronic archive is achieved by integrating the database and dynamically associating the database with the counter serial number.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of breeding technology, in particular to a seed sample cutting mother and sample corresponding type collection coding system. BACKGROUND

[0002] In the process of high-throughput gene analysis of molecular breeding, a large number of seeds need to be sectioned and sampled, and each cut sample must be accurately matched with its mother seed to ensure the accuracy and traceability of subsequent genetic data. The application number CN202011616909.1 discloses a biological breeding sectioning machine. The existing sectioning equipment has the problems of fixed structure, inconvenient adjustment of sectioning interval, poor universality, inability to fix the position of biological seeds during sectioning, poor stability, and low sectioning efficiency. To solve these problems, the following scheme is proposed. It includes a sectioning table, two connecting seats are fixedly installed on the top of the sectioning table, a moving hole is formed on one side of each connecting seat, a same moving plate is slidably installed in the two moving holes, a plurality of adjusting grooves are formed in the bottom of the moving plate, an adjusting plate is slidably installed in each adjusting groove, and a blade is fixedly installed at the bottom of each adjusting plate. The sectioning machine has a reasonable structure and is easy to operate. The sectioning equipment has flexible structure, the sectioning interval is convenient to adjust, has good universality, can meet different sectioning requirements, can stabilize the position of biological seeds during sectioning, has good stability, and has high sectioning efficiency.

[0003] The existing technology mainly focuses on solving the problems of sectioning physical operation itself, such as adjusting the sectioning interval, fixing the position of seeds to improve the sectioning efficiency and stability. However, such equipment has a significant defect: they lack the ability to automatically and highly reliably correspond the samples and the mother after sectioning.

[0004] At present, the correspondence between the sample and the mother depends on manual marking, sub-packaging and recording. This method is inefficient in large-scale sample cutting scenarios and is prone to confusion. Once the sample and the mother corresponding relationship is lost or wrong, the entire batch of experimental data will lose value, causing serious time and resource waste. Therefore, there is an urgent need in the field for a coding collection system that can be integrated into an automatic sample cutting device, which can automatically and accurately establish and record the corresponding relationship between the sample and the mother. SUMMARY

[0005] In order to overcome the defects in the prior art, the purpose of the present application is to provide a seed sample cutting mother and sample corresponding type collection coding system. On the basis of the original laser equipment, by introducing coding control logic and position synchronization mechanism, the samples cut from the same seed and the remaining mother are automatically and accurately collected into the collection box grid with unique corresponding relationship, and traceable coding records are generated, to solve the problems raised in the background art.

[0006] In order to achieve the above-mentioned purpose, the application provides a seed sample cutting mother sample and sample corresponding type collection coding system, which is applied to a laser sample cutting equipment, the equipment comprises a sample collection group for collecting samples and a body collection group for collecting mother samples, the sample collection group comprises a first movement module and a plurality of sample boxes, and the body collection group comprises a second movement module and a plurality of collection boxes; The system comprises a movement control system, a counter and a cutting completion signal module; the movement control system is electrically connected with the first movement module and the second movement module; The movement control system is configured to control the first movement module to support the sample box and control the second movement module to support the first grid position of the collection box to be located directly below the initial collection position; the value of the counter is set to N, and the initial value is 1; after the driving instruction is generated according to the cutting completion signal each time, the value N of the counter is increased by 1; wherein the current value N of the counter indicates the serial number of the current effective grid of the sample box and the collection box; The cutting completion signal module generates a driving instruction after laser cutting, synchronously controls the first movement module and the second movement module to act, and drives the collection positions of the sample box and the collection box to move to the next grid position synchronously; and then the sample generated by one cutting and the mother sample are correspondingly collected in the grid with the same serial number of the sample box and the collection box.

[0007] As a further improvement of the technical solution, the movement control system further comprises a database for storing records; wherein the records comprise batch identifiers, grid serial numbers and seed category information; after the value N of the counter is increased each time, a new record associated with the current grid serial number N is created.

[0008] As a further improvement of the technical solution, the movement control system further comprises a seed waste signal module, if a seed waste signal is received, the response to the cutting completion signal module is inhibited, and no driving instruction is generated.

[0009] As a further improvement of the technical solution, the movement control system further comprises a photoelectric sensor located on the sample falling path, for detecting whether there is sample falling, wherein the driving instruction generated by the cutting completion signal module is generated when the photoelectric sensor detects the sample falling.

[0010] As a further improvement of the technical solution, the first movement module and the second movement module are both XY two-axis movement modules, wherein the driving instruction is used to control the XY two-axis movement module to move along the X axis or the Y axis by a distance of one grid.

[0011] As a further improvement to this technical solution, the sample box and the collection box are multi-cell array boxes, with the cells arranged in a matrix; wherein the driving command is used to control the sample collection group to move all cells sequentially in a row-first or column-first order for collection.

[0012] As a further improvement to this technical solution, the first motion module and the second motion module have the same structure, both consisting of a servo motor and a lead screw connected coaxially, and a slider is threaded onto the lead screw; the top surface of the slider is disc-shaped and used to place several sample boxes and several collection boxes.

[0013] As a further improvement to this technical solution, a turntable is provided above the first motion module and the second motion module. Several pneumatic grippers are installed in a ring at equal intervals on the outer side of the turntable. The turntable rotates intermittently at a fixed angle by emitting pulses from a servo motor.

[0014] As a further improvement to this technical solution, the following steps are included: S1. Initialize the first motion module and the second motion module so that the initial collection position is located at the first cell position of the sample box and the collection box, so as to be directly below the resting position of the pneumatic gripper; S2. When the photoelectric sensor detects that the sample has fallen, it responds to the cutting completion signal module and synchronously drives the first motion module and the second motion module, so that the collection position of the sample box and the collection box moves synchronously to the next grid. S3. Collect the sample and parent tissue generated from the first cut into the grids aligned with the sample box and collection box mentioned before the move.

[0015] As a further improvement to this technical solution, in step S2, the initial value of counter N is 1; after each synchronous drive, the value of N is increased by 1; wherein, in step S3, the sample and the parent are collected in the cell with serial number N, and after the drive, the collection position is aligned with the cell with serial number N+1.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This seed cutting and maternal parent corresponding collection coding system, through the setting of a synchronous motion control system and counter triggered by the cutting signal, completes the synchronous linkage drive and precise site coding of the sample box and maternal parent collection box, achieving the technical effect that after a successful cutting, the sample and maternal parent automatically fall into the same sequence number physical grid; and by integrating a database and dynamically associating it with the counter sequence number, it completes the automatic recording of batch, sequence number, variety and other information of each sample-maternal parent pair, achieving the technical effect of establishing a traceable electronic archive, providing an accurate data traceability foundation for subsequent gene analysis.

[0017] 2. This seed cutting parent and sample corresponding collection and coding system, by introducing discard signal identification and step suppression logic, completes the screening and filtering of invalid cutting events, achieving the technical effect of ensuring absolute consistency between the counter sequence number, physical grid position, and database record, greatly improving the reliability of the system and the accuracy of the data.

[0018] 3. The seed cutting parent plant and sample corresponding collection coding system automatically generates a cutting completion signal through photoelectric sensors, and automatically senses the successful drop of the sample. It achieves the technical effect of driving the system process with physical events, replacing manual intervention or program delay judgment, and further improves the automation level and response accuracy of the system. Attached Figure Description

[0019] The accompanying drawings described herein are for illustrative purposes only and are not intended to limit the scope of the invention in any way. Furthermore, the shapes and proportions of the components in the drawings are merely illustrative to aid in understanding the invention and do not specifically limit the shapes and proportions of the components. Those skilled in the art, guided by the teachings of this invention, will select various possible shapes and proportions to implement the invention according to specific circumstances.

[0020] Figure 1 This is a schematic diagram of the workflow of the present invention; Figure 2 This is a schematic diagram of the overall assembly structure of the present invention; Figure 3 This is a schematic diagram of the sample collection group structure of the present invention; Figure 4 This is a schematic diagram of the main body collection group structure of the present invention; The meanings of the labels in the diagram are as follows: 100. Turntable; 110. Pneumatic gripper; 200. Sample collection group; 210. Sample box; 220. First motion module; 300. Body collection group; 310. Collection box; 320. Second motion module. Detailed Implementation

[0021] The details of the present invention can be more clearly understood by referring to the accompanying drawings and the description of specific embodiments. However, the specific embodiments of the present invention described herein are for illustrative purposes only and should not be construed as limiting the invention in any way. Under the teachings of this invention, those skilled in the art will conceive of any possible variations of the invention, all of which should be considered within the scope of the invention. The terms "installation" and "connection" should be interpreted broadly, referring to direct connection as well as indirect connection through an intermediate medium.

[0022] The terms "central axis," "vertical," "horizontal," "front," "rear," "upper," "lower," "left," "right," "top," "bottom," "inner," and "outer" used herein to indicate orientation or positional relationships are based on the orientation or positional relationships shown in the accompanying drawings and are used only for the convenience of describing the invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, in the description of the invention, "a number" means two or more, unless otherwise explicitly specified.

[0023] Please see Figures 1-4 As shown, the present invention provides a seed cutting parent and sample corresponding collection coding system, which is applied to a laser cutting device. The device includes a sample collection group 200 for collecting samples and a body collection group 300 for collecting parent plants. The sample collection group 200 includes a first motion module 220 and a plurality of sample boxes 210, and the body collection group 300 includes a second motion module 320 and a plurality of collection boxes 310. Specifically, the system includes a motion control system, a counter, and a cutting completion signal module; stored in a computer program, the motion control system is electrically connected to the first motion module 220 and the second motion module 320; The motion control system is configured to: control the first motion module 220 to support the sample box 210 and control the second motion module 320 to support the collection box 310 so that the first cell position is directly below the initial collection position; the counter value is set to N, and its initial value is 1; after each time a drive command is generated according to the cutting completion signal, the counter value N is incremented by 1; wherein, the current value N of the counter indicates the sequence number of the currently valid cell of the sample box 210 and the collection box 310; After laser cutting, the cutting completion signal module generates a drive command to synchronously control the first motion module 220 and the second motion module 320 to move synchronously to the next cell position of the sample box 210 and the collection box 310; thereby realizing that the sample and the parent material produced by one cutting are collected in the cells with the same number in the sample box 210 and the collection box 310.

[0024] Furthermore, the motion control system also includes a database for storing records; these records include batch identifiers, grid numbers, and seed type information; each time the counter value N increases, a new record is created associated with the current grid number N; the motion control system records associated information, such as batch identifiers, grid number N, seed type, cutting parameters, and timestamps, in a database; Subsequently, researchers only need to record the batch identifier and grid number to find the corresponding parent and sample information from the database, or to find the paired sample and parent from the physical collection box by the grid number.

[0025] Furthermore, the motion control system also includes a seed rejection signal module. If a seed rejection signal is received, the response to the cutting completion signal module is suppressed, and no drive command is generated. During the preceding laser cutting of the seed, the vision system identifies the posture of the seed clamping and determines whether it can be cut. If the seed is judged to be unqualified and rejected at the posture recognition station or during the cutting process, the motion control system will recognize this "invalid cut" and will not trigger the stepping command of the box. This ensures that the counter N only counts successful and valid cutting events, guaranteeing absolute consistency between the physical grid and the data record.

[0026] Furthermore, the motion control system also includes photoelectric sensors located on the sample's falling path to detect whether a sample is falling. The drive command generated by the cutting completion signal module is produced by the photoelectric sensors when they detect that a sample is falling.

[0027] Furthermore, the sample box 210 and the collection box 310 are multi-cell array boxes, with their cells arranged in a matrix; the driving instructions are used to control the sample collection group 200 to move all cells sequentially for collection in a row-first or column-first order.

[0028] Furthermore, the first motion module 220 and the second motion module 320 have the same structure, both consisting of a servo motor and a lead screw connected coaxially, and a slider is threaded onto the lead screw; this is prior art and will not be described in detail here; the top surface of the slider is disc-shaped and is used to place several sample boxes 210 and several collection boxes 310.

[0029] Furthermore, both the first motion module 220 and the second motion module 320 are XY two-axis motion modules, wherein the drive command is used to control the XY two-axis motion module to move a distance of one grid along the X-axis or Y-axis; a turntable 100 is provided above the first motion module 220 and the second motion module 320, and several pneumatic grippers 110 are installed in a ring at equal intervals on the outer side of the turntable 100. The turntable 100 rotates intermittently at a fixed angle by emitting pulses from a servo motor. When the turntable 100 stops, the paused position of the pneumatic grippers 110 and located above the first motion module 220 and the second motion module 320 forms two collection positions for dropping seed samples and parent plants.

[0030] The seed cutting parent plant and sample correspondence collection and coding system of the present invention includes the following steps: S1. Initialize the first motion module 220 and the second motion module 320 so that the initial collection position is located at the first cell position of the sample box 210 and the collection box 310, so as to be directly below the resting position of the pneumatic gripper 110. After the system is started or the box is replaced, the motion control system controls the first motion module 220 and the second motion module 320 to reset, positioning the sample box 210 and the collection box 310 in the "1" grid position respectively; the motion control system generates a unique batch identifier and initializes a counter, N=1, which simultaneously points to the currently valid grid of the sample box 210 and the collection box 310.

[0031] S2. When the photoelectric sensor detects that a sample has fallen, it synchronously drives the first motion module 220 and the second motion module 320 in response to the cutting completion signal module, so that the collection positions of the sample box 210 and the collection box 310 move synchronously to the next grid; the initial value of the counter N is 1; after each synchronous drive, the value of N is increased by 1. When a seed is laser-cut at the cutting station, two products are produced: the cut sample and the remaining parent plant. The pneumatic gripper 110 releases, and the sample falls due to gravity, about to fall into the sample box 210. The parent plant is then transported to the next station by the turntable 100, where the pneumatic gripper 110 releases, and the parent plant falls into the collection box 310. Thus, a successful cutting action triggers a stepping command for the box.

[0032] S3. Collect the sample and parent material generated from one cutting into the cells aligned with the sample box 210 and collection box 310 before the movement; the sample and parent material are collected in the cell with serial number N, and after the drive, the collection position is aligned with the cell with serial number N+1. Whenever a parent sample is placed into the Nth cell of the collection box 310, the motion control system immediately and synchronously drives the first motion module 220 and the second motion module 320, causing the N+1th cell of the sample box 210 and the collection box 310 to move simultaneously to the collection position, which is directly below the pneumatic gripper 110 when it stops feeding. The counter N then increments by one. Therefore, the sample and parent sample produced by the Nth cut will necessarily be collected in the Nth cell of the sample box 210 and the collection box 310, respectively. The cell number N itself forms the most direct and reliable correspondence code.

[0033] It should be noted that the above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be used to limit the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A seed cutting parent and sample corresponding collection coding system, applied to a laser cutting device, the device including a sample collection group (200) for collecting samples and a body collection group (300) for collecting parent plants, the sample collection group (200) including a first motion module (220) and a plurality of sample boxes (210), the body collection group (300) including a second motion module (320) and a plurality of collection boxes (310). Its features are, The system includes a motion control system, a counter, and a cutting completion signal module; the motion control system is electrically connected to the first motion module (220) and the second motion module (320); The motion control system is configured to: control the first motion module (220) to support the sample box (210) and control the second motion module (320) to support the first cell position of the collection box (310) directly below the initial collection position; the value of the counter is set to N, and its initial value is 1; each time a drive command is generated according to the cutting completion signal, the value N of the counter is increased by 1; wherein, the current value N of the counter indicates the sequence number of the currently valid cell of the sample box (210) and the collection box (310); The cutting completion signal module generates a driving command after laser cutting, which synchronously controls the first motion module (220) and the second motion module (320) to move synchronously to the next cell position of the sample box (210) and the collection box (310); thereby realizing that the sample and the parent are collected in the same cell of the sample box (210) and the collection box (310) with the same number.

2. The seed cutting parent plant and sample correspondence collection and coding system according to claim 1, characterized in that... The motion control system also includes a database for storing records; the records include batch identifiers, grid numbers, and seed category information; each time the value N of the counter increases, a new record associated with the current grid number N is created.

3. The seed cutting parent plant and sample correspondence collection and coding system according to claim 2, characterized in that: The motion control system also includes a seed discard signal module. If a seed discard signal is received, the response to the cutting completion signal module is suppressed, and no drive command is generated.

4. The seed cutting parent plant and sample correspondence collection coding system according to claim 3, characterized in that: The motion control system also includes a photoelectric sensor located on the sample falling path to detect whether a sample is falling. The drive command generated by the cutting completion signal module is generated by the photoelectric sensor when it detects that a sample is falling.

5. The seed cutting parent plant and sample correspondence collection coding system according to claim 4, characterized in that: The first motion module (220) and the second motion module (320) are both XY two-axis motion modules, wherein the drive command is used to control the XY two-axis motion module to move a distance of one grid along the X-axis or Y-axis.

6. The seed cutting parent plant and sample correspondence collection and coding system according to claim 5, characterized in that: The sample box (210) and the collection box (310) are multi-cell array boxes, and their cells are arranged in a matrix; wherein the driving command is used to control the sample collection group (200) to move all cells in a row-first or column-first order for collection.

7. The seed cutting parent plant and sample correspondence collection coding system according to claim 6, characterized in that: The first motion module (220) and the second motion module (320) have the same structure, both consisting of a servo motor and a lead screw connected coaxially, and a slider is threaded onto the lead screw; the top surface of the slider is disc-shaped and used to place several sample boxes (210) and several collection boxes (310).

8. The seed cutting parent plant and sample correspondence collection coding system according to claim 7, characterized in that: A turntable (100) is provided above the first motion module (220) and the second motion module (320). Several pneumatic grippers (110) are installed in a ring at equal intervals on the outer side of the turntable (100). The turntable (100) rotates intermittently at a fixed angle by emitting pulses from a servo motor.

9. The seed cutting parent plant and sample correspondence collection and coding system according to claim 8, characterized in that: Includes the following steps: S1. Initialize the first motion module (220) and the second motion module (320) so that the initial collection position is located at the first cell position of the sample box (210) and the collection box (310) to be aligned directly below the resting position of the pneumatic gripper (110); S2. When the photoelectric sensor detects that the sample has fallen, it responds to the cutting completion signal module and synchronously drives the first motion module (220) and the second motion module (320) to move the collection positions of the sample box (210) and the collection box (310) to the next grid. S3. Collect the sample and parent material generated from the first cutting into the grids aligned with the sample box (210) and collection box (310) mentioned before the move.

10. The seed cutting parent plant and sample correspondence collection and coding system according to claim 9, characterized in that: In step S2, the initial value of counter N is 1; after each synchronous drive, the value of N is increased by 1; in step S3, the sample and the parent are collected in the cell with serial number N, and after the drive, the collection position is aligned with the cell with serial number N+1.

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