Glucanase and glucose oxidase compound composition and application thereof in preventing and treating plant diseases

Abstract

The invention discloses a dextranase and glucose oxidase compound composition and application thereof in preventing and treating plant diseases, and belongs to the technical field of agricultural biology. Effective components of the compound composition are glucanase and glucose oxidase, and the active unit ratio of the glucanase to the glucose oxidase is (10: 1)-(1: 2). The composition can be applied in a foliar spraying or root irrigation mode and is used for preventing and treating powdery mildew, gray mold, downy mildew and canker of plants. The composition disclosed by the invention realizes synergistic interaction through combination of direct decomposition of enzyme and induction of plants to generate systematic resistance, has a control effect obviously superior to that of a single enzyme preparation, has the advantages of safety, environmental protection, difficulty in generating drug resistance and the like, and is suitable for green and organic agricultural production.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of agricultural biotechnology, and in particular to a complex biological pesticide composition based on glucanase and glucose oxidase, and its application in the prevention and treatment of fungal and bacterial diseases of plants, in particular powdery mildew, gray mold, downy mildew and bacterial blight. BACKGROUND

[0002] Plant diseases are an important factor restricting agricultural production, among which powdery mildew, gray mold, downy mildew and bacterial blight are particularly serious. At present, prevention and treatment mainly relies on chemical pesticides, but long-term use can lead to problems such as drug resistance of pathogenic bacteria, pesticide residues and environmental pollution.

[0003] Biological control has become a research hotspot due to its safety and environmental protection. Glucanase can degrade the main component of fungal cell wall, β-1, 3-glucan, thereby inhibiting the growth of pathogenic bacteria; glucose oxidase can catalyze glucose to produce gluconic acid and hydrogen peroxide, which can directly kill pathogenic bacteria and stimulate plant systemic resistance (SAR). However, single enzyme preparations often have problems such as unstable prevention and treatment effect and narrow spectrum of action. At present, there is no public report on the specific proportion of glucanase and glucose oxidase, and there is a lack of effective application scheme. SUMMARY

[0004] The purpose of the present application is to overcome the shortcomings of the prior art, and to provide a synergistic, safe and environmentally friendly complex composition of glucanase and glucose oxidase and its application, which is used for effectively preventing and treating powdery mildew, gray mold, downy mildew and bacterial blight of plants.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows: a complex composition for preventing and treating plant diseases, the effective components of which are glucanase and glucose oxidase, wherein the activity unit ratio of the glucanase to the glucose oxidase is (10:1) to (1:2), and the preferred ratio is (5:1) to (1:1).

[0006] Further, the composition can also include an agriculturally acceptable adjuvant, and be prepared into wettable powder, water dispersible granules, water agent or suspension concentrate.

[0007] The present application also claims the application of the complex composition in preventing and treating plant diseases, including powdery mildew, gray mold, bacterial blight, downy mildew caused by fungi, and bacterial blight caused by bacteria.

[0008] Further, the application method is: after the complex composition is diluted 300-800 times with water, foliar spraying or root irrigation treatment is carried out; when used for prevention, the dilution multiple is 500-800, and the application interval is 7-15 days; when used for treatment, the dilution multiple is 300-500, and the application interval is 5-7 days.

[0009] The application has the following advantages:

[0010] Synergistic effect: glucanase directly destroys the cell structure of pathogenic bacteria, glucose oxidase produces bactericidal substances and induces plant resistance, the mechanism of action of the two is complementary, the prevention and treatment effect of the complex on various diseases is significantly higher than that of single enzyme preparation, and a synergistic effect of "1+1>2" is produced.

[0011] Broad-spectrum and high efficiency: the composition has good prevention and treatment effect on various fungal diseases (powdery mildew, gray mold and downy mildew) and bacterial diseases (bacterial wilt), and solves the problem of single target of single biological preparation.

[0012] Safety and environmental protection: the composition is derived from biological enzymes, is easy to degrade, has no residue risk, is safe to crops, livestock and the environment, and meets the production requirements of green and organic agriculture.

[0013] Not easy to produce drug resistance: the composition acts through multiple targets, is not easy to induce drug resistance of pathogenic bacteria, and has a long effective period.

[0014] BRIEF DESCRIPTION OF DRAWINGS Figure 1 The comparison chart of the prevention and treatment effect of the complex composition on strawberry gray mold is a comparison chart of the prevention and treatment effect of the complex composition on strawberry gray mold in Example 2 of the application.

[0015]

DETAILED DESCRIPTION

[0016] Example 1: Prevention and treatment test of the complex composition on cucumber powdery mildew The test was carried out in a sunlight greenhouse in Weifang City, Shandong Province, the cucumber variety was 'Jinchun No. 2', and the pesticide was applied at the initial stage of powdery mildew.

[0017] The following treatments were set: A. The complex composition (glucanase: glucose oxidase = 3:1 active units) 500 times liquid of the application; B. 50% glucanase wettable powder 500 times liquid (comparative example); C. 30% glucose oxidase water agent 500 times liquid (comparative example); D. Common chemical agent kresoxim-methyl (control agent) 1500 times liquid; E. Water control (blank control). Each treatment was repeated 4 times, a conventional spraying method was used, and the front and back of the leaves were uniformly sprayed. The disease condition was investigated 14 days after application, and the disease index and control effect were calculated.

[0018] The results show that the prevention and treatment effect (85.4%) of the complex composition is significantly higher than those (66.5% and 62.6%) of the two single enzyme preparations, which proves the synergistic effect, and the effect is close to that of the chemical agent, but is safer.

[0019] Example 2: Prevention and treatment test of the complex composition on strawberry gray mold (similar structure, test design, grouping, results are described, and the effectiveness on gray mold is proved) The following treatments are set: A. The complex composition (glucanase: glucose oxidase = 3:1 active units) 500 times liquid; B. 50% glucanase wettable powder 500 times liquid (comparative example); C. 30% glucose oxidase water agent 500 times liquid (comparative example); D. Common chemical control agent (boscalid) 1000 times liquid; E. Water control (blank control). Each treatment is repeated 4 times, and the conventional spraying method is used to uniformly spray the front and back of the leaves. The disease condition is investigated 14 days after application, and the disease index and prevention and treatment effect are calculated.

[0020] The results show that the prevention and treatment effect (82.5%) of the complex composition is significantly higher than those (64.1% and 55.6%) of the two single enzyme preparations, which proves the synergistic effect, and the effect is close to that of the chemical agent, but is safer.

[0021] Example 3: Prevention and treatment test of the complex composition on citrus canker (similar structure, test design, grouping, results are described, and the effectiveness on bacterial canker is proved)

[0022] The following treatments are set: A. The complex composition (glucanase: glucose oxidase = 3:1 active units) 500 times liquid; B. 50% glucanase wettable powder 500 times liquid (comparative example); C. 30% glucose oxidase water agent 500 times liquid (comparative example); D. Common chemical control agent (thiabendazole) 1000 times liquid; E. Water control (blank control). Each treatment is repeated 4 times, and the conventional spraying method is used to uniformly spray the front and back of the leaves. The disease condition is investigated 21 days after application, and the disease index and prevention and treatment effect are calculated.

[0023] The results show that the prevention and treatment effect (72.4%) of the complex composition is significantly higher than those (65.1% and 52.2%) of the two single enzyme preparations, which proves the synergistic effect, and the effect is close to that of the chemical agent, but is safer.

[0024] Example 4: Prevention and treatment test of the complex composition on cucumber downy mildew (similar structure, test design, grouping, results are described, and the effectiveness on cucumber downy mildew is proved)

[0025] The following treatments were set: A. 500 times solution of the inventive complex composition (glucanase: glucose oxidase = 3:1 activity units); B. 500 times solution of 50% glucanase wettable powder (comparative example); C. 500 times solution of 30% glucose oxidase water agent (comparative example); D. 1000 times solution of a commonly used chemical control agent (oxymil); E. water control (blank control). Each treatment was repeated 4 times, and a conventional spraying method was used to uniformly spray the front and back of the leaves. The disease condition was investigated 10 days after the application, and the disease index and control effect were calculated.

[0026] The results show that the control effect (84.7%) of the inventive complex composition is significantly higher than that of the two single enzyme preparations (66.8% and 61.0%), proving the synergistic effect, and the effect is close to that of the chemical agent, but is more secure.

[0027] Effect summary: The four embodiment quantity contents of the present application prove that the inventive complex composition and its synergistic effect in preventing and treating plant diseases, and the effect is close to that of the chemical agent, but is more secure.

Claims

1. A compound composition for preventing and controlling plant diseases, characterized in that, Its active ingredients are glucanase and glucose oxidase.

2. The compound composition according to claim 1, characterized in that, The ratio of active units of the glucanase to glucose oxidase is (10:1) to (1:2).

3. The compound composition according to claim 2, characterized in that, The ratio of active units of the glucanase to glucose oxidase is (5:1) to (1:1).

4. The compound composition according to any one of claims 1-3, characterized in that, The composition also includes agriculturally acceptable adjuvants, and is formulated as a wettable powder, water-dispersible granule, aqueous solution, or suspension.

5. The use of the compound composition according to any one of claims 1-4 in the preparation of a drug for preventing and controlling plant diseases.

6. The application according to claim 5, characterized in that, The plant diseases mentioned include powdery mildew, gray mold, downy mildew, and canker.

7. The application according to claim 5 or 6, characterized in that, The application method is as follows: dilute the compound composition with water 300-800 times and then perform foliar spraying or root irrigation.

8. The application according to claim 7, characterized in that, When used for prevention, the dilution ratio is 500-800 times, and the application interval is 7-15 days.

9. The application according to claim 7, characterized in that, When used for treatment, the dilution ratio is 300-500 times, and the application interval is 5-7 days.

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